buffer 1  (New England Biolabs)


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    • 93
    Name:
    NEBuffer 1 1
    Description:
    NEBuffer 1 1 5 0 ml
    Catalog Number:
    b7201s
    Price:
    24
    Size:
    5 0 ml
    Category:
    Buffers
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    Structured Review

    New England Biolabs buffer 1
    NEBuffer 1 1
    NEBuffer 1 1 5 0 ml
    https://www.bioz.com/result/buffer 1/product/New England Biolabs
    Average 93 stars, based on 42 article reviews
    Price from $9.99 to $1999.99
    buffer 1 - by Bioz Stars, 2021-02
    93/100 stars

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    Centrifugation:

    Article Title: Analysis of the Interaction between Globular Head Modules of Human C1q and Its Candidate Receptor gC1qR
    Article Snippet: .. After centrifugation (13,000 rpm for 15 min), the supernatant was collected and diluted fivefold in buffer I (20 mM Tris–HCl, pH 8.0, 100 mM NaCl, 0.2% Tween 20, 1 mM EDTA, and 5% v/v glycerol) and passed through an amylose resin 15 ml bed column (New England Biolabs). ..

    Article Title: Complement Protein C1q Interacts with DC-SIGN via Its Globular Domain and Thus May Interfere with HIV-1 Transmission
    Article Snippet: .. After centrifugation (13,000 × g for 15 min), the supernatant was diluted fivefold in buffer I (20 mM Tris–HCl, pH 8.0, 100 mM NaCl, 0.2% Tween 20, 1 mM EDTA, and 5% glycerol), passed through an amylose resin column (15 mL; New England Biolabs), and then washed with three bed volumes of buffer I followed by buffer II (50 mL of buffer I without Tween 20). .. The protein was then eluted in 1 mL fractions with 10 mM maltose in 100 mL of buffer II and frozen at −20°C after determining protein concentration and purity via Nanodrop and 10% w/v SDS-PAGE, respectively.

    Agarose Gel Electrophoresis:

    Article Title: A Simple Procedure for Creating Scalable Phenotypic Screening Assays in Human Neurons
    Article Snippet: .. Individual plasmid DNA were incubated with appropriate restriction enzymes (New England Biolabs, Ipswich, MA) and NE Buffer (cat. no. B7201, NEB) for 1 h. Purple loading dye (cat. no. B7024S, NEB) was added to each sample which were loaded carefully on MiniReady Agarose Gel prepared in 1XTBE (cat. no. 101-3004, Biorad) alongside with Quick load purple 1 kb DNA ladder as a reference standard (cat. no. N0552G, NEB). .. The results demonstrated appropriate digest products for each plasmid as well as the maxi-prep RFLP and can be found in the supplemental data (Figure ).

    Isolation:

    Article Title: The Helicobacter pylori HpyAXII restriction-modification system limits exogenous DNA uptake by targeting GTAC sites but shows asymmetric conservation of the DNA methyltransferase and restriction endonuclease components
    Article Snippet: .. Biochemical R.HpyAXII endonuclease activity assay One microliter of freshly prepared soluble fraction obtained from E. coli BL21(DE3) expressing R.HpyAXII was incubated for 3 h at 37°C with 500 ng E. coli ER2925 genomic DNA or pET15b plasmid DNA isolated from E. coli DH10B in presence of NEB buffer 1 (New England Biolabs). ..

    Purification:

    Article Title: ‘Shotgun DNA synthesis’ for the high-throughput construction of large DNA molecules
    Article Snippet: .. For each pool, restriction enzyme digestion was carried out by incubating 2 µl EarI (or EcoP15I), 5 µl NEB buffer 1, 0.5 µl 100× BSA and 10 µl water with 30 µl of purified (and pooled) shotgun synthesis fragments at 37°C for 3 h. For EcoP15I (NEB, Ipswich, MA, USA) restriction enzyme digestion, we used NEB buffer 3 and added 10× ATP. ..

    Incubation:

    Article Title: A Simple Procedure for Creating Scalable Phenotypic Screening Assays in Human Neurons
    Article Snippet: .. Individual plasmid DNA were incubated with appropriate restriction enzymes (New England Biolabs, Ipswich, MA) and NE Buffer (cat. no. B7201, NEB) for 1 h. Purple loading dye (cat. no. B7024S, NEB) was added to each sample which were loaded carefully on MiniReady Agarose Gel prepared in 1XTBE (cat. no. 101-3004, Biorad) alongside with Quick load purple 1 kb DNA ladder as a reference standard (cat. no. N0552G, NEB). .. The results demonstrated appropriate digest products for each plasmid as well as the maxi-prep RFLP and can be found in the supplemental data (Figure ).

    Article Title: The Helicobacter pylori HpyAXII restriction-modification system limits exogenous DNA uptake by targeting GTAC sites but shows asymmetric conservation of the DNA methyltransferase and restriction endonuclease components
    Article Snippet: .. Biochemical R.HpyAXII endonuclease activity assay One microliter of freshly prepared soluble fraction obtained from E. coli BL21(DE3) expressing R.HpyAXII was incubated for 3 h at 37°C with 500 ng E. coli ER2925 genomic DNA or pET15b plasmid DNA isolated from E. coli DH10B in presence of NEB buffer 1 (New England Biolabs). ..

    Article Title: A novel use of random priming-based single-strand library preparation for whole genome sequencing of formalin-fixed paraffin-embedded tissue samples
    Article Snippet: .. WGS library preparation—DDAT protocol To remove damaged bases, 2 ng of good or poor quality FFPE DNA and 10 ng of very poor quality DNA were combined with 5 U of SMUG1, 1 U Fpg, 1× NEB buffer 1 and 0.1 μg/ml bovine serum albumin (NEB) in 10 μl and incubated for 1 h at 37°C. (This enzyme digestion step was excluded in the pilot experiment.) ..

    Formalin-fixed Paraffin-Embedded:

    Article Title: A novel use of random priming-based single-strand library preparation for whole genome sequencing of formalin-fixed paraffin-embedded tissue samples
    Article Snippet: .. WGS library preparation—DDAT protocol To remove damaged bases, 2 ng of good or poor quality FFPE DNA and 10 ng of very poor quality DNA were combined with 5 U of SMUG1, 1 U Fpg, 1× NEB buffer 1 and 0.1 μg/ml bovine serum albumin (NEB) in 10 μl and incubated for 1 h at 37°C. (This enzyme digestion step was excluded in the pilot experiment.) ..

    Activity Assay:

    Article Title: The Helicobacter pylori HpyAXII restriction-modification system limits exogenous DNA uptake by targeting GTAC sites but shows asymmetric conservation of the DNA methyltransferase and restriction endonuclease components
    Article Snippet: .. Biochemical R.HpyAXII endonuclease activity assay One microliter of freshly prepared soluble fraction obtained from E. coli BL21(DE3) expressing R.HpyAXII was incubated for 3 h at 37°C with 500 ng E. coli ER2925 genomic DNA or pET15b plasmid DNA isolated from E. coli DH10B in presence of NEB buffer 1 (New England Biolabs). ..

    Expressing:

    Article Title: The Helicobacter pylori HpyAXII restriction-modification system limits exogenous DNA uptake by targeting GTAC sites but shows asymmetric conservation of the DNA methyltransferase and restriction endonuclease components
    Article Snippet: .. Biochemical R.HpyAXII endonuclease activity assay One microliter of freshly prepared soluble fraction obtained from E. coli BL21(DE3) expressing R.HpyAXII was incubated for 3 h at 37°C with 500 ng E. coli ER2925 genomic DNA or pET15b plasmid DNA isolated from E. coli DH10B in presence of NEB buffer 1 (New England Biolabs). ..

    Plasmid Preparation:

    Article Title: A Simple Procedure for Creating Scalable Phenotypic Screening Assays in Human Neurons
    Article Snippet: .. Individual plasmid DNA were incubated with appropriate restriction enzymes (New England Biolabs, Ipswich, MA) and NE Buffer (cat. no. B7201, NEB) for 1 h. Purple loading dye (cat. no. B7024S, NEB) was added to each sample which were loaded carefully on MiniReady Agarose Gel prepared in 1XTBE (cat. no. 101-3004, Biorad) alongside with Quick load purple 1 kb DNA ladder as a reference standard (cat. no. N0552G, NEB). .. The results demonstrated appropriate digest products for each plasmid as well as the maxi-prep RFLP and can be found in the supplemental data (Figure ).

    Article Title: The Helicobacter pylori HpyAXII restriction-modification system limits exogenous DNA uptake by targeting GTAC sites but shows asymmetric conservation of the DNA methyltransferase and restriction endonuclease components
    Article Snippet: .. Biochemical R.HpyAXII endonuclease activity assay One microliter of freshly prepared soluble fraction obtained from E. coli BL21(DE3) expressing R.HpyAXII was incubated for 3 h at 37°C with 500 ng E. coli ER2925 genomic DNA or pET15b plasmid DNA isolated from E. coli DH10B in presence of NEB buffer 1 (New England Biolabs). ..

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    • buffer 1  (New England Biolabs)
    93
    New England Biolabs buffer 1
    Buffer 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/buffer 1/product/New England Biolabs
    Average 93 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    buffer 1 - by Bioz Stars, 2021-02
    93/100 stars
    		  Buy from Supplier

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