bp dna ladder  (New England Biolabs)


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  • 99
    Name:
    100 bp DNA Ladder
    Description:
    100 bp DNA Ladder 500 gel lanes
    Catalog Number:
    N3231L
    Price:
    230
    Size:
    500 gel lanes
    Category:
    DNA Ladders
    Score:
    85
    Buy from Supplier
    Name:
    50 bp DNA Ladder
    Description:
    50 bp DNA Ladder 500 1000 gel lanes
    Catalog Number:
    N3236L
    Price:
    280
    Size:
    1000 gel lanes
    Category:
    DNA Ladders
    Score:
    85
    Buy from Supplier


    Structured Review

    New England Biolabs bp dna ladder
    50 bp DNA Ladder
    50 bp DNA Ladder 500 1000 gel lanes
    https://www.bioz.com/result/bp dna ladder/product/New England Biolabs
    Average 99 stars, based on 64 article reviews
    Price from $9.99 to $1999.99
    bp dna ladder - by Bioz Stars, 2019-10
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    Related Articles

    Amplification:

    Article Title: Genotyping bacterial and fungal pathogens using sequence variation in the gene for the CCA-adding enzyme
    Article Snippet: Hence, the blocking oligonucleotide can increase the selective amplification of pathogenic loop sequences in the PCR reaction. .. N, PCR negative control; M, 50 bp DNA ladder (NEB). (DOCX 4696 kb) Additional file 2: Table S1.

    Article Title: Lactobacilli with probiotic potential in the prairie vole (Microtus ochrogaster)
    Article Snippet: Paragraph title: Random amplified polymorphic DNA (RAPD) fingerprinting ... PCR amplicons were separated by gel electrophoresis using 1.5 % high resolution agarose gels in 1× Tris–Acetate-EDTA buffer with a 100 bp DNA ladder (New England Biolabs, Ipswich, MA, USA) as size marker.

    Article Title: Specific Magnetic Isolation of E6 HPV16 Modified Magnetizable Particles Coupled with PCR and Electrochemical Detection
    Article Snippet: Paragraph title: 4.3. Isolation and PCR Amplification of E6 Human Papillomavirus 16 ... The 100 bp DNA ladder (New England Biolabs, Ispwich, MA, USA) was used as a molecule size marker.

    Article Title: Molecular typing of Streptococcus suis strains isolated from diseased and healthy pigs between 1996-2016
    Article Snippet: Paragraph title: Supporting information Geographic origin of samples from which S . suis was isolated. Specificity of rec N primers. TEM images of serologically and genetically non-typable S . suis isolates. Primers for rec N amplification integrated into the two-step multiplex PCR by Okura et al. (J Clin Microbiol. 2014;52(5):1714–9.). Serotypes of German S . suis isolates based on cps typing in collection A (1996–2004) and collection B (2015–2016), respectively, grouped by disease status. Serotypes of German invasive S . suis isolates based on cps typing in collection A (1996–2004) and collection B (2015–2016), respectively, grouped by origin of isolation. Comparison of molecular and phenotypic serotyping for a representative selection of S . suis isolates. Pathotype of selected serotypes (ST) based on cps typing of invasive, pulmonary and carrier S . suis isolates from collection A (1996–2004) and collection B (2015–2016). Characterization of the capsule of serologically and genetically non-typable S . suis isolates from collection B as revealed from TEM images. ... ; lane 17 100 bp DNA Ladder (NEB). (PDF) Click here for additional data file.

    Article Title: Improvement of Rotavirus Genotyping Method by Using the Semi-Nested Multiplex-PCR With New Primer Set
    Article Snippet: A 100 bp DNA ladder (New England BioLabs, Ipswich, MA, United States) was used as DNA size marker. .. A 100 bp DNA ladder (New England BioLabs, Ipswich, MA, United States) was used as DNA size marker.

    Article Title: Extensive diversity in the allelic frequency of Plasmodium falciparum merozoite surface proteins and glutamate-rich protein in rural and urban settings of southwestern Nigeria
    Article Snippet: The polymorphic allelic families of msp -1 (K1, MAD20, and RO33) and the block 3 region of msp -2 (FC27 and 3D7) genes were amplified with a nested PCR amplification in a final reaction volume of 20 µl containing 10X PCR buffer (20 mM Tris–HCl [pH 8.4], 50 mM KCl, and 1.5 mM of MgCl2), 0.125 mM of dNTPs, 0.25 mM of each primer, and 1 U Taq DNA polymerase (New England Biolabs. .. DNA sizes were determined using 100 bp DNA ladder (New England Biolabs.

    Article Title: Prevalence and types of high-risk human papillomaviruses in head and neck cancers from Bangladesh
    Article Snippet: The PCR products of the samples from both rounds were electrophoresed in 2% agarose gel prepared with 1× TAE (Tris-acetate-EDTA) buffer (DNA Agar, Marine Bio Products Inc., Quincy, MA, USA), stained with 0.5 g/mol of ethidium bromide (Merck, KGaA, Darmstadt, Germany) and visualized under ultraviolet light using the Chemi-Doc machine (BioRad, USA). .. The size of the amplified product was determined by comparing with a reference molecular weight DNA marker, (Quick Load, 100 bp DNA Ladder, New England Biolab, MA, USA). .. Any sample that showed a positive band in the gel for both first (band size 450 bp) and second (band size 150 bp) round of PCR was taken as an HPV-positive sample and purified for sequencing.

    Article Title: Possible role of thymidine phosphorylase in gynecological tumors as an individualized treatment strategy
    Article Snippet: In addition, β-actin was amplified with another pair of primers (5′-TCATGAAGTGTGACGTTGACATCCGT-3′ and 5′-CCTAGAAGCATTTGCGGTGCACGATG-3′; Promega Corporation), and served as internal standard. .. Tris-acetate-EDTA (50X; Sigma-Aldrich) was used as the running buffer and 100 bp DNA Ladder (New England Biolabs Japan, Inc., Tokyo, Japan) was used as a marker.

    Article Title: Genome-driven evaluation and redesign of PCR tools for improving the detection of virulence-associated genes in aeromonads
    Article Snippet: The amplification conditions performed on a GeneAmp PCR System 9700 (Applied Biosystems, Foster city, US) were as follows: initial denaturation for 3 min at 95°C, followed by (1) main amplification program of the reference source specified in the for already published PCR, with length of extension adapted to the efficacy of the DNA polymerase used (1 min/kb) when necessary, and a final extension step at 72°C for 10 min or (2) 32 amplification cycles as indicated in for newly developed PCR. .. The PCR products and the 50 bp DNA ladder (New England BioLabs, Ipswich, US) were separated in 1.5% agarose gels in 0.5X TBE buffer-ethidium bromide 500 μg/mL and revealed under UV.

    Article Title: Water, water everywhere: environmental DNA can unlock population structure in elusive marine species
    Article Snippet: Products from all three PCRs were pooled and the presence of the desired amplicon size was confirmed by electrophoresis of the pooled 30 µl in a 2% agarose gel stained with SyberSafe (ThermoFisher, Waltham, MA). .. Amplicon size was determined with reference to a 100 bp DNA ladder (New England Biolabs, Ipswich, MA). .. Two negative control reactions were run in each set of PCR reactions, including template from one extraction blank and a no-template PCR sample.

    Positive Control:

    Article Title: Nitrogen regulation of protein-protein interactions and transcript levels of GlnK PII regulator and AmtB ammonium transporter homologs in Archaea
    Article Snippet: A positive control with genomic DNA and negative controls without cDNA or with cDNA obtained from RT reactions lacking retrotranscriptase were also included. .. PCR fragments were visualized in 1.8% (w/v) agarose gels stained with ethidium bromide and compared to the 100 bp DNA Ladder from New England Biolabs (Ipswich, MA).

    Article Title: Genome-driven evaluation and redesign of PCR tools for improving the detection of virulence-associated genes in aeromonads
    Article Snippet: The PCR products and the 50 bp DNA ladder (New England BioLabs, Ipswich, US) were separated in 1.5% agarose gels in 0.5X TBE buffer-ethidium bromide 500 μg/mL and revealed under UV. .. Specificity of the assays was confirmed using PCR-grade water and DNA from A . fluvialis LMG 24681T which harbors in its genome none of the genes of interest.

    Synthesized:

    Article Title: Nitrogen regulation of protein-protein interactions and transcript levels of GlnK PII regulator and AmtB ammonium transporter homologs in Archaea
    Article Snippet: Regular PCR (Taq polymerase; Bioline, London, UK) was used to amplify the synthesized DNA with specific primers for amtB1 and amtB2 (primers listed in ), which were designed in nonhomologous sequence regions from both genes ( ). .. PCR fragments were visualized in 1.8% (w/v) agarose gels stained with ethidium bromide and compared to the 100 bp DNA Ladder from New England Biolabs (Ipswich, MA).

    Blocking Assay:

    Article Title: Genotyping bacterial and fungal pathogens using sequence variation in the gene for the CCA-adding enzyme
    Article Snippet: Hence, the blocking oligonucleotide can increase the selective amplification of pathogenic loop sequences in the PCR reaction. .. N, PCR negative control; M, 50 bp DNA ladder (NEB). (DOCX 4696 kb) Additional file 2: Table S1.

    Article Title: Extensive diversity in the allelic frequency of Plasmodium falciparum merozoite surface proteins and glutamate-rich protein in rural and urban settings of southwestern Nigeria
    Article Snippet: The polymorphic allelic families of msp -1 (K1, MAD20, and RO33) and the block 3 region of msp -2 (FC27 and 3D7) genes were amplified with a nested PCR amplification in a final reaction volume of 20 µl containing 10X PCR buffer (20 mM Tris–HCl [pH 8.4], 50 mM KCl, and 1.5 mM of MgCl2), 0.125 mM of dNTPs, 0.25 mM of each primer, and 1 U Taq DNA polymerase (New England Biolabs. .. DNA sizes were determined using 100 bp DNA ladder (New England Biolabs.

    SYBR Green Assay:

    Article Title: Extensive diversity in the allelic frequency of Plasmodium falciparum merozoite surface proteins and glutamate-rich protein in rural and urban settings of southwestern Nigeria
    Article Snippet: The resultant PCR products were stained with SYBR® Green 1 nucleic acid gel stain (Cambrex Biosciences, East Rutherford, NJ, USA) and resolved by gel electrophoresis in 1.5% agarose gel. .. DNA sizes were determined using 100 bp DNA ladder (New England Biolabs.

    Modification:

    Article Title: Water, water everywhere: environmental DNA can unlock population structure in elusive marine species
    Article Snippet: Replicate 10 µl reactions were performed for all eDNA and control samples, using optimized conditions (as above) and modified primers containing Illumina sequencing primer and adapter sequences. .. Amplicon size was determined with reference to a 100 bp DNA ladder (New England Biolabs, Ipswich, MA).

    Sequencing:

    Article Title: Genotyping bacterial and fungal pathogens using sequence variation in the gene for the CCA-adding enzyme
    Article Snippet: Increasing ratios of blocking oligonucleotide versus forward primer inhibit amplification of the human sequence, while the bacterial sequence amplification remains unaffected. .. N, PCR negative control; M, 50 bp DNA ladder (NEB). (DOCX 4696 kb) Additional file 2: Table S1.

    Article Title: Nitrogen regulation of protein-protein interactions and transcript levels of GlnK PII regulator and AmtB ammonium transporter homologs in Archaea
    Article Snippet: Regular PCR (Taq polymerase; Bioline, London, UK) was used to amplify the synthesized DNA with specific primers for amtB1 and amtB2 (primers listed in ), which were designed in nonhomologous sequence regions from both genes ( ). .. PCR fragments were visualized in 1.8% (w/v) agarose gels stained with ethidium bromide and compared to the 100 bp DNA Ladder from New England Biolabs (Ipswich, MA).

    Article Title: Water, water everywhere: environmental DNA can unlock population structure in elusive marine species
    Article Snippet: Paragraph title: Next-generation eDNA amplicon sequencing ... Amplicon size was determined with reference to a 100 bp DNA ladder (New England Biolabs, Ipswich, MA).

    Molecular Weight:

    Article Title: Characterization of a DNA Adenine Methyltransferase Gene of Borrelia hermsii and Its Dispensability for Murine Infection and Persistence
    Article Snippet: DpnI and MboI cleave sequences that are adenine methylated and adenine non-methylated, respectively. .. Sizes of selected molecular weight standards (mws) of 100 bp DNA ladder (New England Biolabs) are shown on the left in base pairs. (TIF) Click here for additional data file. .. S2 Fig DNA from Bh Δdam #2 is either undigested, or digested with DpnI, MboI, or Sau3AI.

    Article Title: Prevalence and types of high-risk human papillomaviruses in head and neck cancers from Bangladesh
    Article Snippet: The PCR products of the samples from both rounds were electrophoresed in 2% agarose gel prepared with 1× TAE (Tris-acetate-EDTA) buffer (DNA Agar, Marine Bio Products Inc., Quincy, MA, USA), stained with 0.5 g/mol of ethidium bromide (Merck, KGaA, Darmstadt, Germany) and visualized under ultraviolet light using the Chemi-Doc machine (BioRad, USA). .. The size of the amplified product was determined by comparing with a reference molecular weight DNA marker, (Quick Load, 100 bp DNA Ladder, New England Biolab, MA, USA). .. Any sample that showed a positive band in the gel for both first (band size 450 bp) and second (band size 150 bp) round of PCR was taken as an HPV-positive sample and purified for sequencing.

    Northern Blot:

    Article Title: Nitrogen regulation of protein-protein interactions and transcript levels of GlnK PII regulator and AmtB ammonium transporter homologs in Archaea
    Article Snippet: PCR fragments were visualized in 1.8% (w/v) agarose gels stained with ethidium bromide and compared to the 100 bp DNA Ladder from New England Biolabs (Ipswich, MA). .. PCR fragments were visualized in 1.8% (w/v) agarose gels stained with ethidium bromide and compared to the 100 bp DNA Ladder from New England Biolabs (Ipswich, MA).

    Infection:

    Article Title: Characterization of a DNA Adenine Methyltransferase Gene of Borrelia hermsii and Its Dispensability for Murine Infection and Persistence
    Article Snippet: Paragraph title: Supporting Information Bh Δdam remains Dam deficient on day 10 post inoculation. Verification of Dam disruption in Bh Δdam #2 by restriction enzyme digestion. In vitro growth curves of wild type B . hermsii and Bh Δdam #2. Partial nucleotide alignment of the Bh Δdam variable membrane protein (vmp ) prior to infection compared to the sequences of vmp from Bh Δdam recovered from 3 mice on day 10 post infection. Mean spirochete density as determined by qPCR for wild type B . hermsii and Bh Δdam at days 3, 7, and 10 post inoculation. Infection of SCID mice with wild type B . hermsii and Bh Δdam at decreasing infectious doses of approximately 1 x 105 to 10 spirochetes. Infection by a second, independently generated clone of Bh Δdam (Bh Δdam #2) in B6 and SCID mice as determined by blood culture. ... Sizes of selected molecular weight standards (mws) of 100 bp DNA ladder (New England Biolabs) are shown on the left in base pairs. (TIF) Click here for additional data file.

    Generated:

    Article Title: Characterization of a DNA Adenine Methyltransferase Gene of Borrelia hermsii and Its Dispensability for Murine Infection and Persistence
    Article Snippet: Paragraph title: Supporting Information Bh Δdam remains Dam deficient on day 10 post inoculation. Verification of Dam disruption in Bh Δdam #2 by restriction enzyme digestion. In vitro growth curves of wild type B . hermsii and Bh Δdam #2. Partial nucleotide alignment of the Bh Δdam variable membrane protein (vmp ) prior to infection compared to the sequences of vmp from Bh Δdam recovered from 3 mice on day 10 post infection. Mean spirochete density as determined by qPCR for wild type B . hermsii and Bh Δdam at days 3, 7, and 10 post inoculation. Infection of SCID mice with wild type B . hermsii and Bh Δdam at decreasing infectious doses of approximately 1 x 105 to 10 spirochetes. Infection by a second, independently generated clone of Bh Δdam (Bh Δdam #2) in B6 and SCID mice as determined by blood culture. ... Sizes of selected molecular weight standards (mws) of 100 bp DNA ladder (New England Biolabs) are shown on the left in base pairs. (TIF) Click here for additional data file.

    Article Title: Molecular typing of Streptococcus suis strains isolated from diseased and healthy pigs between 1996-2016
    Article Snippet: S1 Fig A: collection A (1996–2004); B: collection B (2015–2016) Legend: number of isolates Note: high numbers of isolates correspond to areas with dense pig population The map was generated with our data using the software package “Das Postleitzahlen-Diagramm 4.0” by Klaus Wessiepe ( http://www.Klaus-Wessiepe.de ) licensed for „Institut für Mikrobiologie, Tierrztliche Hochschule Hannover“, 2007. (PDF) Click here for additional data file. .. ; lane 17 100 bp DNA Ladder (NEB). (PDF) Click here for additional data file.

    Article Title: Water, water everywhere: environmental DNA can unlock population structure in elusive marine species
    Article Snippet: Positive controls were generated using genomic DNA from four Sanger sequenced, vouchered harbour porpoise samples to test for PCR or sequencing error. .. Amplicon size was determined with reference to a 100 bp DNA ladder (New England Biolabs, Ipswich, MA).

    other:

    Article Title: Intravesicle Isothermal DNA Replication
    Article Snippet: The IsoAmp tHDA kit and 50 bp DNA ladder were from New England BioLabs.

    Size-exclusion Chromatography:

    Article Title: Possible role of thymidine phosphorylase in gynecological tumors as an individualized treatment strategy
    Article Snippet: PCR was initiated with denaturation at 95°C for 10 min, followed by 30 cycles of 95°C for 20 sec, 59°C for 30 sec and 72°C for 60 sec, with a final elongation at 72°C for 10 min. PCR products were analyzed by 2% agarose gel electrophoresis (Wako Pure Chemical Industries, Ltd.), and the gels were stained with ethidium bromide (Invitrogen; Thermo Fisher Scientific, Inc.). .. Tris-acetate-EDTA (50X; Sigma-Aldrich) was used as the running buffer and 100 bp DNA Ladder (New England Biolabs Japan, Inc., Tokyo, Japan) was used as a marker.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Nitrogen regulation of protein-protein interactions and transcript levels of GlnK PII regulator and AmtB ammonium transporter homologs in Archaea
    Article Snippet: For RT-PCR analyses (reverse transcription PCR), RNA samples were digested twice for 30 min at 37°C with the RNase-Free DNase Set from QIAGEN to avoid DNA contamination. .. PCR fragments were visualized in 1.8% (w/v) agarose gels stained with ethidium bromide and compared to the 100 bp DNA Ladder from New England Biolabs (Ipswich, MA).

    Article Title: Intravenous Delivery of Oncolytic Reovirus to Brain Tumor Patients Immunologically Primes for Subsequent Checkpoint Blockade
    Article Snippet: Paragraph title: Peripheral reovirus carriage RT-PCR ... Samples were run on 2 % agarose gels alongside a 100 bp DNA ladder (New England Biolabs).

    Article Title: Possible role of thymidine phosphorylase in gynecological tumors as an individualized treatment strategy
    Article Snippet: Paragraph title: Reverse transcription-polymerase chain reaction (RT-PCR) analysis ... Tris-acetate-EDTA (50X; Sigma-Aldrich) was used as the running buffer and 100 bp DNA Ladder (New England Biolabs Japan, Inc., Tokyo, Japan) was used as a marker.

    Binding Assay:

    Article Title: Genotyping bacterial and fungal pathogens using sequence variation in the gene for the CCA-adding enzyme
    Article Snippet: This interferes with binding of the PCR forward primer (green), and, consequently, amplification of the human sequence. .. N, PCR negative control; M, 50 bp DNA ladder (NEB). (DOCX 4696 kb) Additional file 2: Table S1.

    Magnetic Cell Separation:

    Article Title: Intravenous Delivery of Oncolytic Reovirus to Brain Tumor Patients Immunologically Primes for Subsequent Checkpoint Blockade
    Article Snippet: PBMC subsets were isolated by MACS Microbead selection (Miltenyi Biotec) according to manufacturer’s instructions. .. Samples were run on 2 % agarose gels alongside a 100 bp DNA ladder (New England Biolabs).

    Nucleic Acid Electrophoresis:

    Article Title: Lactobacilli with probiotic potential in the prairie vole (Microtus ochrogaster)
    Article Snippet: The probiotic strains L. johnsonii ATCC 33200 and L. rhamnosus GG were used as references. .. PCR amplicons were separated by gel electrophoresis using 1.5 % high resolution agarose gels in 1× Tris–Acetate-EDTA buffer with a 100 bp DNA ladder (New England Biolabs, Ipswich, MA, USA) as size marker. .. Gels were stained with SYBR Safe DNA gel stain (Life Technologies) and scanned with a Typhoon 9410 Variable Mode Imager (GE Healthcare Biosciences, Pittsburgh, PA, USA).

    Article Title: Extensive diversity in the allelic frequency of Plasmodium falciparum merozoite surface proteins and glutamate-rich protein in rural and urban settings of southwestern Nigeria
    Article Snippet: The resultant PCR products were stained with SYBR® Green 1 nucleic acid gel stain (Cambrex Biosciences, East Rutherford, NJ, USA) and resolved by gel electrophoresis in 1.5% agarose gel. .. DNA sizes were determined using 100 bp DNA ladder (New England Biolabs.

    Article Title: Water, water everywhere: environmental DNA can unlock population structure in elusive marine species
    Article Snippet: Amplicon size was determined with reference to a 100 bp DNA ladder (New England Biolabs, Ipswich, MA). .. Bands from successful PCR of the eDNA samples and positive controls were gel excised using clean techniques to prevent cross-contamination between amplicons and purified using the Qiagen MinElute Gel Extraction spin-column protocol with a 30 µl elution volume (Qiagen, Germantown, MD).

    Fluorescence:

    Article Title: ELISA-like Analysis of Cisplatinated DNA Using Magnetic Separation
    Article Snippet: A 100 bp DNA ladder (New England BioLabs, Ipswich, MA, USA) within the size range from 0.1 to 1.5 kb was used to monitor the size of the analysed fragment. .. A 100 bp DNA ladder (New England BioLabs, Ipswich, MA, USA) within the size range from 0.1 to 1.5 kb was used to monitor the size of the analysed fragment.

    Methylation:

    Article Title: Characterization of a DNA Adenine Methyltransferase Gene of Borrelia hermsii and Its Dispensability for Murine Infection and Persistence
    Article Snippet: DpnI and MboI cleave sequences that are adenine methylated and adenine non-methylated, respectively. .. Sizes of selected molecular weight standards (mws) of 100 bp DNA ladder (New England Biolabs) are shown on the left in base pairs. (TIF) Click here for additional data file.

    Isolation:

    Article Title: Intravenous Delivery of Oncolytic Reovirus to Brain Tumor Patients Immunologically Primes for Subsequent Checkpoint Blockade
    Article Snippet: PBMC subsets were isolated by MACS Microbead selection (Miltenyi Biotec) according to manufacturer’s instructions. .. Samples were run on 2 % agarose gels alongside a 100 bp DNA ladder (New England Biolabs).

    Article Title: Specific Magnetic Isolation of E6 HPV16 Modified Magnetizable Particles Coupled with PCR and Electrochemical Detection
    Article Snippet: Paragraph title: 4.3. Isolation and PCR Amplification of E6 Human Papillomavirus 16 ... The 100 bp DNA ladder (New England Biolabs, Ispwich, MA, USA) was used as a molecule size marker.

    Article Title: Molecular typing of Streptococcus suis strains isolated from diseased and healthy pigs between 1996-2016
    Article Snippet: Paragraph title: Supporting information Geographic origin of samples from which S . suis was isolated. Specificity of rec N primers. TEM images of serologically and genetically non-typable S . suis isolates. Primers for rec N amplification integrated into the two-step multiplex PCR by Okura et al. (J Clin Microbiol. 2014;52(5):1714–9.). Serotypes of German S . suis isolates based on cps typing in collection A (1996–2004) and collection B (2015–2016), respectively, grouped by disease status. Serotypes of German invasive S . suis isolates based on cps typing in collection A (1996–2004) and collection B (2015–2016), respectively, grouped by origin of isolation. Comparison of molecular and phenotypic serotyping for a representative selection of S . suis isolates. Pathotype of selected serotypes (ST) based on cps typing of invasive, pulmonary and carrier S . suis isolates from collection A (1996–2004) and collection B (2015–2016). Characterization of the capsule of serologically and genetically non-typable S . suis isolates from collection B as revealed from TEM images. ... ; lane 17 100 bp DNA Ladder (NEB). (PDF) Click here for additional data file.

    Multiplex PCR:

    Article Title: Improvement of Rotavirus Genotyping Method by Using the Semi-Nested Multiplex-PCR With New Primer Set
    Article Snippet: Paragraph title: Genotyping by Semi-Nested Multiplex-PCR ... A 100 bp DNA ladder (New England BioLabs, Ipswich, MA, United States) was used as DNA size marker.

    AST Assay:

    Article Title: Genome-driven evaluation and redesign of PCR tools for improving the detection of virulence-associated genes in aeromonads
    Article Snippet: Both selected and new primers were used to amplify DNA from aerolysin/enterotoxin cytotoxic (aerA /act ), heat-stable cytotonic enterotoxin (ast ), heat-labile cytotonic enterotoxin (alt ), lipase (lip ), serine protease (ser ), ADP-ribosylating toxins (aexT and aexU ), shiga toxins 1 (stx1 ) and 2 (stx2a ), T3SS needle proteins (ascFG ), T3SS inner membrane channel protein (ascV ) and lateral flagellin A (lafA ) genes. .. The PCR products and the 50 bp DNA ladder (New England BioLabs, Ipswich, US) were separated in 1.5% agarose gels in 0.5X TBE buffer-ethidium bromide 500 μg/mL and revealed under UV.

    Negative Control:

    Article Title: Genotyping bacterial and fungal pathogens using sequence variation in the gene for the CCA-adding enzyme
    Article Snippet: Hence, the blocking oligonucleotide can increase the selective amplification of pathogenic loop sequences in the PCR reaction. .. N, PCR negative control; M, 50 bp DNA ladder (NEB). (DOCX 4696 kb) Additional file 2: Table S1. .. Strains used for analysis of intraspecific similarities in the gene region encoding the loop sequence.

    Mouse Assay:

    Article Title: Characterization of a DNA Adenine Methyltransferase Gene of Borrelia hermsii and Its Dispensability for Murine Infection and Persistence
    Article Snippet: Paragraph title: Supporting Information Bh Δdam remains Dam deficient on day 10 post inoculation. Verification of Dam disruption in Bh Δdam #2 by restriction enzyme digestion. In vitro growth curves of wild type B . hermsii and Bh Δdam #2. Partial nucleotide alignment of the Bh Δdam variable membrane protein (vmp ) prior to infection compared to the sequences of vmp from Bh Δdam recovered from 3 mice on day 10 post infection. Mean spirochete density as determined by qPCR for wild type B . hermsii and Bh Δdam at days 3, 7, and 10 post inoculation. Infection of SCID mice with wild type B . hermsii and Bh Δdam at decreasing infectious doses of approximately 1 x 105 to 10 spirochetes. Infection by a second, independently generated clone of Bh Δdam (Bh Δdam #2) in B6 and SCID mice as determined by blood culture. ... Sizes of selected molecular weight standards (mws) of 100 bp DNA ladder (New England Biolabs) are shown on the left in base pairs. (TIF) Click here for additional data file.

    Polymerase Chain Reaction:

    Article Title: Genotyping bacterial and fungal pathogens using sequence variation in the gene for the CCA-adding enzyme
    Article Snippet: Hence, the blocking oligonucleotide can increase the selective amplification of pathogenic loop sequences in the PCR reaction. .. N, PCR negative control; M, 50 bp DNA ladder (NEB). (DOCX 4696 kb) Additional file 2: Table S1. .. Strains used for analysis of intraspecific similarities in the gene region encoding the loop sequence.

    Article Title: Nitrogen regulation of protein-protein interactions and transcript levels of GlnK PII regulator and AmtB ammonium transporter homologs in Archaea
    Article Snippet: A positive control with genomic DNA and negative controls without cDNA or with cDNA obtained from RT reactions lacking retrotranscriptase were also included. .. PCR fragments were visualized in 1.8% (w/v) agarose gels stained with ethidium bromide and compared to the 100 bp DNA Ladder from New England Biolabs (Ipswich, MA). .. Cells were harvested by centrifugation at 5500g for 15 min at 4°C (Avanti J-20 XP Centrifuge; Beckman Coulter, Brea, CA) and disrupted passing them twice at 2000 psi through a cooled French press (SLM AMINCO, Urbana, IL).

    Article Title: Lactobacilli with probiotic potential in the prairie vole (Microtus ochrogaster)
    Article Snippet: The probiotic strains L. johnsonii ATCC 33200 and L. rhamnosus GG were used as references. .. PCR amplicons were separated by gel electrophoresis using 1.5 % high resolution agarose gels in 1× Tris–Acetate-EDTA buffer with a 100 bp DNA ladder (New England Biolabs, Ipswich, MA, USA) as size marker. .. Gels were stained with SYBR Safe DNA gel stain (Life Technologies) and scanned with a Typhoon 9410 Variable Mode Imager (GE Healthcare Biosciences, Pittsburgh, PA, USA).

    Article Title: Intravenous Delivery of Oncolytic Reovirus to Brain Tumor Patients Immunologically Primes for Subsequent Checkpoint Blockade
    Article Snippet: Samples were run on 2 % agarose gels alongside a 100 bp DNA ladder (New England Biolabs). .. Samples were run on 2 % agarose gels alongside a 100 bp DNA ladder (New England Biolabs).

    Article Title: Specific Magnetic Isolation of E6 HPV16 Modified Magnetizable Particles Coupled with PCR and Electrochemical Detection
    Article Snippet: Paragraph title: 4.3. Isolation and PCR Amplification of E6 Human Papillomavirus 16 ... The 100 bp DNA ladder (New England Biolabs, Ispwich, MA, USA) was used as a molecule size marker.

    Article Title: Molecular typing of Streptococcus suis strains isolated from diseased and healthy pigs between 1996-2016
    Article Snippet: Paragraph title: Supporting information Geographic origin of samples from which S . suis was isolated. Specificity of rec N primers. TEM images of serologically and genetically non-typable S . suis isolates. Primers for rec N amplification integrated into the two-step multiplex PCR by Okura et al. (J Clin Microbiol. 2014;52(5):1714–9.). Serotypes of German S . suis isolates based on cps typing in collection A (1996–2004) and collection B (2015–2016), respectively, grouped by disease status. Serotypes of German invasive S . suis isolates based on cps typing in collection A (1996–2004) and collection B (2015–2016), respectively, grouped by origin of isolation. Comparison of molecular and phenotypic serotyping for a representative selection of S . suis isolates. Pathotype of selected serotypes (ST) based on cps typing of invasive, pulmonary and carrier S . suis isolates from collection A (1996–2004) and collection B (2015–2016). Characterization of the capsule of serologically and genetically non-typable S . suis isolates from collection B as revealed from TEM images. ... ; lane 17 100 bp DNA Ladder (NEB). (PDF) Click here for additional data file.

    Article Title: Improvement of Rotavirus Genotyping Method by Using the Semi-Nested Multiplex-PCR With New Primer Set
    Article Snippet: Second PCR was performed using Premix Ex TaqTM Hot Start Version (Takara) with second primers (5 pmol each). .. A 100 bp DNA ladder (New England BioLabs, Ipswich, MA, United States) was used as DNA size marker.

    Article Title: Extensive diversity in the allelic frequency of Plasmodium falciparum merozoite surface proteins and glutamate-rich protein in rural and urban settings of southwestern Nigeria
    Article Snippet: The resultant PCR products were stained with SYBR® Green 1 nucleic acid gel stain (Cambrex Biosciences, East Rutherford, NJ, USA) and resolved by gel electrophoresis in 1.5% agarose gel. .. DNA sizes were determined using 100 bp DNA ladder (New England Biolabs.

    Article Title: Reprogramming of Adult Peripheral Blood Cells into Human Induced Pluripotent Stem Cells as a Safe and Accessible Source of Endothelial Cells
    Article Snippet: The PCR products of Ig/TCR genes were ultraviolet-visualized on 2% ethidium bromide-stained agarose gel. .. The presence of the expected size product was checked based on a 100 bp DNA Ladder (New England Biolabs, Ipswich, MA).

    Article Title: Prevalence and types of high-risk human papillomaviruses in head and neck cancers from Bangladesh
    Article Snippet: Paragraph title: Detection of HPV DNA by PCR ... The size of the amplified product was determined by comparing with a reference molecular weight DNA marker, (Quick Load, 100 bp DNA Ladder, New England Biolab, MA, USA).

    Article Title: Genetic variation and DNA fingerprinting of durian types in Malaysia using simple sequence repeat (SSR) markers
    Article Snippet: Paragraph title: Selection of SSR primers and detection of PCR products ... The DNA fragment sizes were estimated by comparison of sample banding patterns with a 50 bp DNA ladder (New England Biolabs Inc., Ipswich, MA, USA) loaded in the same gel.

    Article Title: Genome-driven evaluation and redesign of PCR tools for improving the detection of virulence-associated genes in aeromonads
    Article Snippet: The amplification conditions performed on a GeneAmp PCR System 9700 (Applied Biosystems, Foster city, US) were as follows: initial denaturation for 3 min at 95°C, followed by (1) main amplification program of the reference source specified in the for already published PCR, with length of extension adapted to the efficacy of the DNA polymerase used (1 min/kb) when necessary, and a final extension step at 72°C for 10 min or (2) 32 amplification cycles as indicated in for newly developed PCR. .. The PCR products and the 50 bp DNA ladder (New England BioLabs, Ipswich, US) were separated in 1.5% agarose gels in 0.5X TBE buffer-ethidium bromide 500 μg/mL and revealed under UV. .. Positive and negative controls were added in each PCR to assess the validity and specificity of amplification reaction.

    Article Title: Water, water everywhere: environmental DNA can unlock population structure in elusive marine species
    Article Snippet: Each sample was amplified in three independent 10 µl reactions to ensure sufficient PCR product for next-generation sequencing. .. Amplicon size was determined with reference to a 100 bp DNA ladder (New England Biolabs, Ipswich, MA).

    Polyacrylamide Gel Electrophoresis:

    Article Title: Methods for targeted mutagenesis in zebrafish using TALENs
    Article Snippet: – 100 bp DNA ladder (New England Biolabs). .. – 100 bp DNA ladder (New England Biolabs).

    Staining:

    Article Title: Nitrogen regulation of protein-protein interactions and transcript levels of GlnK PII regulator and AmtB ammonium transporter homologs in Archaea
    Article Snippet: A positive control with genomic DNA and negative controls without cDNA or with cDNA obtained from RT reactions lacking retrotranscriptase were also included. .. PCR fragments were visualized in 1.8% (w/v) agarose gels stained with ethidium bromide and compared to the 100 bp DNA Ladder from New England Biolabs (Ipswich, MA). .. Cells were harvested by centrifugation at 5500g for 15 min at 4°C (Avanti J-20 XP Centrifuge; Beckman Coulter, Brea, CA) and disrupted passing them twice at 2000 psi through a cooled French press (SLM AMINCO, Urbana, IL).

    Article Title: Extensive diversity in the allelic frequency of Plasmodium falciparum merozoite surface proteins and glutamate-rich protein in rural and urban settings of southwestern Nigeria
    Article Snippet: The resultant PCR products were stained with SYBR® Green 1 nucleic acid gel stain (Cambrex Biosciences, East Rutherford, NJ, USA) and resolved by gel electrophoresis in 1.5% agarose gel. .. DNA sizes were determined using 100 bp DNA ladder (New England Biolabs.

    Article Title: Prevalence and types of high-risk human papillomaviruses in head and neck cancers from Bangladesh
    Article Snippet: The PCR products of the samples from both rounds were electrophoresed in 2% agarose gel prepared with 1× TAE (Tris-acetate-EDTA) buffer (DNA Agar, Marine Bio Products Inc., Quincy, MA, USA), stained with 0.5 g/mol of ethidium bromide (Merck, KGaA, Darmstadt, Germany) and visualized under ultraviolet light using the Chemi-Doc machine (BioRad, USA). .. The size of the amplified product was determined by comparing with a reference molecular weight DNA marker, (Quick Load, 100 bp DNA Ladder, New England Biolab, MA, USA).

    Article Title: Genetic variation and DNA fingerprinting of durian types in Malaysia using simple sequence repeat (SSR) markers
    Article Snippet: PCR amplicons were analyzed through electrophoresis on 8% (w/v) polyacrylamide gels, stained with ethidium bromide and viewed under UV illumination. .. The DNA fragment sizes were estimated by comparison of sample banding patterns with a 50 bp DNA ladder (New England Biolabs Inc., Ipswich, MA, USA) loaded in the same gel.

    Article Title: Water, water everywhere: environmental DNA can unlock population structure in elusive marine species
    Article Snippet: Products from all three PCRs were pooled and the presence of the desired amplicon size was confirmed by electrophoresis of the pooled 30 µl in a 2% agarose gel stained with SyberSafe (ThermoFisher, Waltham, MA). .. Amplicon size was determined with reference to a 100 bp DNA ladder (New England Biolabs, Ipswich, MA).

    Nested PCR:

    Article Title: Extensive diversity in the allelic frequency of Plasmodium falciparum merozoite surface proteins and glutamate-rich protein in rural and urban settings of southwestern Nigeria
    Article Snippet: The polymorphic allelic families of msp -1 (K1, MAD20, and RO33) and the block 3 region of msp -2 (FC27 and 3D7) genes were amplified with a nested PCR amplification in a final reaction volume of 20 µl containing 10X PCR buffer (20 mM Tris–HCl [pH 8.4], 50 mM KCl, and 1.5 mM of MgCl2), 0.125 mM of dNTPs, 0.25 mM of each primer, and 1 U Taq DNA polymerase (New England Biolabs. .. DNA sizes were determined using 100 bp DNA ladder (New England Biolabs.

    Activated Clotting Time Assay:

    Article Title: Genome-driven evaluation and redesign of PCR tools for improving the detection of virulence-associated genes in aeromonads
    Article Snippet: Both selected and new primers were used to amplify DNA from aerolysin/enterotoxin cytotoxic (aerA /act ), heat-stable cytotonic enterotoxin (ast ), heat-labile cytotonic enterotoxin (alt ), lipase (lip ), serine protease (ser ), ADP-ribosylating toxins (aexT and aexU ), shiga toxins 1 (stx1 ) and 2 (stx2a ), T3SS needle proteins (ascFG ), T3SS inner membrane channel protein (ascV ) and lateral flagellin A (lafA ) genes. .. The PCR products and the 50 bp DNA ladder (New England BioLabs, Ipswich, US) were separated in 1.5% agarose gels in 0.5X TBE buffer-ethidium bromide 500 μg/mL and revealed under UV.

    Purification:

    Article Title: Specific Magnetic Isolation of E6 HPV16 Modified Magnetizable Particles Coupled with PCR and Electrochemical Detection
    Article Snippet: The DNA amplification was carried out for 40 cycles of the denaturation at 94 °C for 30 s, the annealing at 56 °C for 30 s and the primer extension at 72 °C for 30 s. The amplified E6-HPV16 oncogene was purified using MiniElute PCR Purification Kit (Qiagen, Germantown, MD, USA). .. The 100 bp DNA ladder (New England Biolabs, Ispwich, MA, USA) was used as a molecule size marker.

    Article Title: Water, water everywhere: environmental DNA can unlock population structure in elusive marine species
    Article Snippet: Amplicon size was determined with reference to a 100 bp DNA ladder (New England Biolabs, Ipswich, MA). .. Two negative control reactions were run in each set of PCR reactions, including template from one extraction blank and a no-template PCR sample.

    Plasmid Preparation:

    Article Title: Specific Magnetic Isolation of E6 HPV16 Modified Magnetizable Particles Coupled with PCR and Electrochemical Detection
    Article Snippet: The PCR mixture (Taq PCR kit, New England Biolabs, Ispwich, MA, USA), contained the PCR buffer (10 mM Tris-HCl pH 8.3, 50 mM KCl with 1.5 mM MgCl2 included), 0.2 mM of dNTPs and 0.4 µM of each primers with E6-HPV16-pUC57 synthetic plasmid using as a template. .. The 100 bp DNA ladder (New England Biolabs, Ispwich, MA, USA) was used as a molecule size marker.

    Software:

    Article Title: Lactobacilli with probiotic potential in the prairie vole (Microtus ochrogaster)
    Article Snippet: PCR amplicons were separated by gel electrophoresis using 1.5 % high resolution agarose gels in 1× Tris–Acetate-EDTA buffer with a 100 bp DNA ladder (New England Biolabs, Ipswich, MA, USA) as size marker. .. Gels were stained with SYBR Safe DNA gel stain (Life Technologies) and scanned with a Typhoon 9410 Variable Mode Imager (GE Healthcare Biosciences, Pittsburgh, PA, USA).

    Article Title: Molecular typing of Streptococcus suis strains isolated from diseased and healthy pigs between 1996-2016
    Article Snippet: S1 Fig A: collection A (1996–2004); B: collection B (2015–2016) Legend: number of isolates Note: high numbers of isolates correspond to areas with dense pig population The map was generated with our data using the software package “Das Postleitzahlen-Diagramm 4.0” by Klaus Wessiepe ( http://www.Klaus-Wessiepe.de ) licensed for „Institut für Mikrobiologie, Tierrztliche Hochschule Hannover“, 2007. (PDF) Click here for additional data file. .. ; lane 17 100 bp DNA Ladder (NEB). (PDF) Click here for additional data file.

    Article Title: ELISA-like Analysis of Cisplatinated DNA Using Magnetic Separation
    Article Snippet: A 100 bp DNA ladder (New England BioLabs, Ipswich, MA, USA) within the size range from 0.1 to 1.5 kb was used to monitor the size of the analysed fragment. .. A 100 bp DNA ladder (New England BioLabs, Ipswich, MA, USA) within the size range from 0.1 to 1.5 kb was used to monitor the size of the analysed fragment.

    Real-time Polymerase Chain Reaction:

    Article Title: Characterization of a DNA Adenine Methyltransferase Gene of Borrelia hermsii and Its Dispensability for Murine Infection and Persistence
    Article Snippet: Paragraph title: Supporting Information Bh Δdam remains Dam deficient on day 10 post inoculation. Verification of Dam disruption in Bh Δdam #2 by restriction enzyme digestion. In vitro growth curves of wild type B . hermsii and Bh Δdam #2. Partial nucleotide alignment of the Bh Δdam variable membrane protein (vmp ) prior to infection compared to the sequences of vmp from Bh Δdam recovered from 3 mice on day 10 post infection. Mean spirochete density as determined by qPCR for wild type B . hermsii and Bh Δdam at days 3, 7, and 10 post inoculation. Infection of SCID mice with wild type B . hermsii and Bh Δdam at decreasing infectious doses of approximately 1 x 105 to 10 spirochetes. Infection by a second, independently generated clone of Bh Δdam (Bh Δdam #2) in B6 and SCID mice as determined by blood culture. ... Sizes of selected molecular weight standards (mws) of 100 bp DNA ladder (New England Biolabs) are shown on the left in base pairs. (TIF) Click here for additional data file.

    Multiplex Assay:

    Article Title: Molecular typing of Streptococcus suis strains isolated from diseased and healthy pigs between 1996-2016
    Article Snippet: Paragraph title: Supporting information Geographic origin of samples from which S . suis was isolated. Specificity of rec N primers. TEM images of serologically and genetically non-typable S . suis isolates. Primers for rec N amplification integrated into the two-step multiplex PCR by Okura et al. (J Clin Microbiol. 2014;52(5):1714–9.). Serotypes of German S . suis isolates based on cps typing in collection A (1996–2004) and collection B (2015–2016), respectively, grouped by disease status. Serotypes of German invasive S . suis isolates based on cps typing in collection A (1996–2004) and collection B (2015–2016), respectively, grouped by origin of isolation. Comparison of molecular and phenotypic serotyping for a representative selection of S . suis isolates. Pathotype of selected serotypes (ST) based on cps typing of invasive, pulmonary and carrier S . suis isolates from collection A (1996–2004) and collection B (2015–2016). Characterization of the capsule of serologically and genetically non-typable S . suis isolates from collection B as revealed from TEM images. ... ; lane 17 100 bp DNA Ladder (NEB). (PDF) Click here for additional data file.

    Article Title: Reprogramming of Adult Peripheral Blood Cells into Human Induced Pluripotent Stem Cells as a Safe and Accessible Source of Endothelial Cells
    Article Snippet: Sixty-three primers were used in seven multiplex PCR tubes to detect (1) complete VH-JH rearrangement of immunoglobulin heavy chain (IGH) gene (three tubes), (2) complete Vβ-Jβ rearrangement of TRB gene (two tubes), and (3) TRG gene rearrangements (two tubes). .. The presence of the expected size product was checked based on a 100 bp DNA Ladder (New England Biolabs, Ipswich, MA).

    Selection:

    Article Title: Intravenous Delivery of Oncolytic Reovirus to Brain Tumor Patients Immunologically Primes for Subsequent Checkpoint Blockade
    Article Snippet: PBMC subsets were isolated by MACS Microbead selection (Miltenyi Biotec) according to manufacturer’s instructions. .. Samples were run on 2 % agarose gels alongside a 100 bp DNA ladder (New England Biolabs).

    Article Title: Molecular typing of Streptococcus suis strains isolated from diseased and healthy pigs between 1996-2016
    Article Snippet: Paragraph title: Supporting information Geographic origin of samples from which S . suis was isolated. Specificity of rec N primers. TEM images of serologically and genetically non-typable S . suis isolates. Primers for rec N amplification integrated into the two-step multiplex PCR by Okura et al. (J Clin Microbiol. 2014;52(5):1714–9.). Serotypes of German S . suis isolates based on cps typing in collection A (1996–2004) and collection B (2015–2016), respectively, grouped by disease status. Serotypes of German invasive S . suis isolates based on cps typing in collection A (1996–2004) and collection B (2015–2016), respectively, grouped by origin of isolation. Comparison of molecular and phenotypic serotyping for a representative selection of S . suis isolates. Pathotype of selected serotypes (ST) based on cps typing of invasive, pulmonary and carrier S . suis isolates from collection A (1996–2004) and collection B (2015–2016). Characterization of the capsule of serologically and genetically non-typable S . suis isolates from collection B as revealed from TEM images. ... ; lane 17 100 bp DNA Ladder (NEB). (PDF) Click here for additional data file.

    Article Title: Genetic variation and DNA fingerprinting of durian types in Malaysia using simple sequence repeat (SSR) markers
    Article Snippet: Paragraph title: Selection of SSR primers and detection of PCR products ... The DNA fragment sizes were estimated by comparison of sample banding patterns with a 50 bp DNA ladder (New England Biolabs Inc., Ipswich, MA, USA) loaded in the same gel.

    Agarose Gel Electrophoresis:

    Article Title: Specific Magnetic Isolation of E6 HPV16 Modified Magnetizable Particles Coupled with PCR and Electrochemical Detection
    Article Snippet: The amplified product of 477 base pairs was analyzed by agarose gel electrophoresis and the conditions were as follows: 1% agarose gel (Agarose MP, Roche Diagnostics, Indianapolis, IN, USA) in TAE buffer, at 60 V for 160 min (Bio-Rad, Hercules, CA, USA). .. The 100 bp DNA ladder (New England Biolabs, Ispwich, MA, USA) was used as a molecule size marker.

    Article Title: Extensive diversity in the allelic frequency of Plasmodium falciparum merozoite surface proteins and glutamate-rich protein in rural and urban settings of southwestern Nigeria
    Article Snippet: The resultant PCR products were stained with SYBR® Green 1 nucleic acid gel stain (Cambrex Biosciences, East Rutherford, NJ, USA) and resolved by gel electrophoresis in 1.5% agarose gel. .. DNA sizes were determined using 100 bp DNA ladder (New England Biolabs.

    Article Title: Reprogramming of Adult Peripheral Blood Cells into Human Induced Pluripotent Stem Cells as a Safe and Accessible Source of Endothelial Cells
    Article Snippet: The PCR products of Ig/TCR genes were ultraviolet-visualized on 2% ethidium bromide-stained agarose gel. .. The presence of the expected size product was checked based on a 100 bp DNA Ladder (New England Biolabs, Ipswich, MA).

    Article Title: Prevalence and types of high-risk human papillomaviruses in head and neck cancers from Bangladesh
    Article Snippet: The PCR products of the samples from both rounds were electrophoresed in 2% agarose gel prepared with 1× TAE (Tris-acetate-EDTA) buffer (DNA Agar, Marine Bio Products Inc., Quincy, MA, USA), stained with 0.5 g/mol of ethidium bromide (Merck, KGaA, Darmstadt, Germany) and visualized under ultraviolet light using the Chemi-Doc machine (BioRad, USA). .. The size of the amplified product was determined by comparing with a reference molecular weight DNA marker, (Quick Load, 100 bp DNA Ladder, New England Biolab, MA, USA).

    Article Title: ELISA-like Analysis of Cisplatinated DNA Using Magnetic Separation
    Article Snippet: Paragraph title: 2.8 Agarose gel electrophoresis ... A 100 bp DNA ladder (New England BioLabs, Ipswich, MA, USA) within the size range from 0.1 to 1.5 kb was used to monitor the size of the analysed fragment.

    Article Title: Water, water everywhere: environmental DNA can unlock population structure in elusive marine species
    Article Snippet: Products from all three PCRs were pooled and the presence of the desired amplicon size was confirmed by electrophoresis of the pooled 30 µl in a 2% agarose gel stained with SyberSafe (ThermoFisher, Waltham, MA). .. Amplicon size was determined with reference to a 100 bp DNA ladder (New England Biolabs, Ipswich, MA).

    In Vitro:

    Article Title: Characterization of a DNA Adenine Methyltransferase Gene of Borrelia hermsii and Its Dispensability for Murine Infection and Persistence
    Article Snippet: Paragraph title: Supporting Information Bh Δdam remains Dam deficient on day 10 post inoculation. Verification of Dam disruption in Bh Δdam #2 by restriction enzyme digestion. In vitro growth curves of wild type B . hermsii and Bh Δdam #2. Partial nucleotide alignment of the Bh Δdam variable membrane protein (vmp ) prior to infection compared to the sequences of vmp from Bh Δdam recovered from 3 mice on day 10 post infection. Mean spirochete density as determined by qPCR for wild type B . hermsii and Bh Δdam at days 3, 7, and 10 post inoculation. Infection of SCID mice with wild type B . hermsii and Bh Δdam at decreasing infectious doses of approximately 1 x 105 to 10 spirochetes. Infection by a second, independently generated clone of Bh Δdam (Bh Δdam #2) in B6 and SCID mice as determined by blood culture. ... Sizes of selected molecular weight standards (mws) of 100 bp DNA ladder (New England Biolabs) are shown on the left in base pairs. (TIF) Click here for additional data file.

    Electrophoresis:

    Article Title: Improvement of Rotavirus Genotyping Method by Using the Semi-Nested Multiplex-PCR With New Primer Set
    Article Snippet: The amplicons were analyzed by electrophoresis on 1.5% agarose gels with ethidium bromide. .. A 100 bp DNA ladder (New England BioLabs, Ipswich, MA, United States) was used as DNA size marker.

    Article Title: Genetic variation and DNA fingerprinting of durian types in Malaysia using simple sequence repeat (SSR) markers
    Article Snippet: PCR amplicons were analyzed through electrophoresis on 8% (w/v) polyacrylamide gels, stained with ethidium bromide and viewed under UV illumination. .. The DNA fragment sizes were estimated by comparison of sample banding patterns with a 50 bp DNA ladder (New England Biolabs Inc., Ipswich, MA, USA) loaded in the same gel.

    Article Title: Water, water everywhere: environmental DNA can unlock population structure in elusive marine species
    Article Snippet: Products from all three PCRs were pooled and the presence of the desired amplicon size was confirmed by electrophoresis of the pooled 30 µl in a 2% agarose gel stained with SyberSafe (ThermoFisher, Waltham, MA). .. Amplicon size was determined with reference to a 100 bp DNA ladder (New England Biolabs, Ipswich, MA).

    Next-Generation Sequencing:

    Article Title: Water, water everywhere: environmental DNA can unlock population structure in elusive marine species
    Article Snippet: Each sample was amplified in three independent 10 µl reactions to ensure sufficient PCR product for next-generation sequencing. .. Amplicon size was determined with reference to a 100 bp DNA ladder (New England Biolabs, Ipswich, MA).

    Concentration Assay:

    Article Title: Methods for targeted mutagenesis in zebrafish using TALENs
    Article Snippet: – A spectrometer for measuring DNA concentration. .. – 100 bp DNA ladder (New England Biolabs).

    Article Title: Water, water everywhere: environmental DNA can unlock population structure in elusive marine species
    Article Snippet: DNA from each control sample was quantified using a Qubit™ 3.0 (ThermoFisher, Waltham, MA) and the Qubit DS Broad Range Assay (ThermoFisher, Waltham, MA) according to the manufacturer's instructions, and samples were normalized to a concentration of 5 ng µl−1 . .. Amplicon size was determined with reference to a 100 bp DNA ladder (New England Biolabs, Ipswich, MA).

    Transmission Electron Microscopy:

    Article Title: Molecular typing of Streptococcus suis strains isolated from diseased and healthy pigs between 1996-2016
    Article Snippet: Paragraph title: Supporting information Geographic origin of samples from which S . suis was isolated. Specificity of rec N primers. TEM images of serologically and genetically non-typable S . suis isolates. Primers for rec N amplification integrated into the two-step multiplex PCR by Okura et al. (J Clin Microbiol. 2014;52(5):1714–9.). Serotypes of German S . suis isolates based on cps typing in collection A (1996–2004) and collection B (2015–2016), respectively, grouped by disease status. Serotypes of German invasive S . suis isolates based on cps typing in collection A (1996–2004) and collection B (2015–2016), respectively, grouped by origin of isolation. Comparison of molecular and phenotypic serotyping for a representative selection of S . suis isolates. Pathotype of selected serotypes (ST) based on cps typing of invasive, pulmonary and carrier S . suis isolates from collection A (1996–2004) and collection B (2015–2016). Characterization of the capsule of serologically and genetically non-typable S . suis isolates from collection B as revealed from TEM images. ... ; lane 17 100 bp DNA Ladder (NEB). (PDF) Click here for additional data file.

    Marker:

    Article Title: Lactobacilli with probiotic potential in the prairie vole (Microtus ochrogaster)
    Article Snippet: The probiotic strains L. johnsonii ATCC 33200 and L. rhamnosus GG were used as references. .. PCR amplicons were separated by gel electrophoresis using 1.5 % high resolution agarose gels in 1× Tris–Acetate-EDTA buffer with a 100 bp DNA ladder (New England Biolabs, Ipswich, MA, USA) as size marker. .. Gels were stained with SYBR Safe DNA gel stain (Life Technologies) and scanned with a Typhoon 9410 Variable Mode Imager (GE Healthcare Biosciences, Pittsburgh, PA, USA).

    Article Title: Specific Magnetic Isolation of E6 HPV16 Modified Magnetizable Particles Coupled with PCR and Electrochemical Detection
    Article Snippet: The amplified product of 477 base pairs was analyzed by agarose gel electrophoresis and the conditions were as follows: 1% agarose gel (Agarose MP, Roche Diagnostics, Indianapolis, IN, USA) in TAE buffer, at 60 V for 160 min (Bio-Rad, Hercules, CA, USA). .. The 100 bp DNA ladder (New England Biolabs, Ispwich, MA, USA) was used as a molecule size marker. .. The ethidium bromide-labeled bands were visualized via UV transilluminator at 312 nm (Vilber-Lourmat, Marne-la-Valle’e Cedex, France).

    Article Title: Improvement of Rotavirus Genotyping Method by Using the Semi-Nested Multiplex-PCR With New Primer Set
    Article Snippet: The amplicons were analyzed by electrophoresis on 1.5% agarose gels with ethidium bromide. .. A 100 bp DNA ladder (New England BioLabs, Ipswich, MA, United States) was used as DNA size marker. .. VP7 nucleotide sequences of representative strains were retrieved from GenBank and aligned using CLUSTAL W, which was included in the MEGA software package, version 7.0.18 and the MAFFT multiple sequence alignment software program, version 7.0 ( ).

    Article Title: Prevalence and types of high-risk human papillomaviruses in head and neck cancers from Bangladesh
    Article Snippet: The PCR products of the samples from both rounds were electrophoresed in 2% agarose gel prepared with 1× TAE (Tris-acetate-EDTA) buffer (DNA Agar, Marine Bio Products Inc., Quincy, MA, USA), stained with 0.5 g/mol of ethidium bromide (Merck, KGaA, Darmstadt, Germany) and visualized under ultraviolet light using the Chemi-Doc machine (BioRad, USA). .. The size of the amplified product was determined by comparing with a reference molecular weight DNA marker, (Quick Load, 100 bp DNA Ladder, New England Biolab, MA, USA). .. Any sample that showed a positive band in the gel for both first (band size 450 bp) and second (band size 150 bp) round of PCR was taken as an HPV-positive sample and purified for sequencing.

    Article Title: Possible role of thymidine phosphorylase in gynecological tumors as an individualized treatment strategy
    Article Snippet: PCR was initiated with denaturation at 95°C for 10 min, followed by 30 cycles of 95°C for 20 sec, 59°C for 30 sec and 72°C for 60 sec, with a final elongation at 72°C for 10 min. PCR products were analyzed by 2% agarose gel electrophoresis (Wako Pure Chemical Industries, Ltd.), and the gels were stained with ethidium bromide (Invitrogen; Thermo Fisher Scientific, Inc.). .. Tris-acetate-EDTA (50X; Sigma-Aldrich) was used as the running buffer and 100 bp DNA Ladder (New England Biolabs Japan, Inc., Tokyo, Japan) was used as a marker. .. Mupid (Advance Co., Ltd., Tokyo, Japan) was used for electrophoresis.

    Article Title: Genetic variation and DNA fingerprinting of durian types in Malaysia using simple sequence repeat (SSR) markers
    Article Snippet: Resultant PCR amplicons for each marker were Sanger-sequenced on an ABI 3730 sequencer, through services provided by First Base Laboratories Sdn Bhd. (Selangor, Malaysia), in order to verify that the amplicons were the targeted regions that contained SSR sequences. .. The DNA fragment sizes were estimated by comparison of sample banding patterns with a 50 bp DNA ladder (New England Biolabs Inc., Ipswich, MA, USA) loaded in the same gel.

    Gel Extraction:

    Article Title: Water, water everywhere: environmental DNA can unlock population structure in elusive marine species
    Article Snippet: Amplicon size was determined with reference to a 100 bp DNA ladder (New England Biolabs, Ipswich, MA). .. Two negative control reactions were run in each set of PCR reactions, including template from one extraction blank and a no-template PCR sample.

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    New England Biolabs bp dna ladder
    Bp Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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