gel loading dye purple 6x no sds  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
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  • 95
    Name:
    Gel Loading Dye Purple 6X no SDS
    Description:
    Gel Loading Dye Purple 6X no SDS 4 0 ml
    Catalog Number:
    b7025s
    Price:
    44
    Size:
    4 0 ml
    Category:
    Electrophoresis Tracking Dyes
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    Structured Review

    New England Biolabs gel loading dye purple 6x no sds
    Gel Loading Dye Purple 6X no SDS
    Gel Loading Dye Purple 6X no SDS 4 0 ml
    https://www.bioz.com/result/gel loading dye purple 6x no sds/product/New England Biolabs
    Average 95 stars, based on 80 article reviews
    Price from $9.99 to $1999.99
    gel loading dye purple 6x no sds - by Bioz Stars, 2020-10
    95/100 stars

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    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: The DNA-binding protein HTa from Thermoplasma acidophilum is an archaeal histone analog
    Article Snippet: .. 4 µL of 6x loading buffer (without SDS, NEB #B7025S) were added just before gel electrophoresis. ..

    Agarose Gel Electrophoresis:

    Article Title: DNA origami demonstrate the unique stimulatory power of single pMHCs as T-cell antigens
    Article Snippet: .. Agarose gel electrophoresis DNA origami were mixed with DNA loading buffer (B7025S, New England Biolabs) and subjected to agarose gel electrophoresis (1% agarose (A9539, Sigma Aldrich), 1x Tris Acetate-EDTA (TAE) (15558042, ThermoFisher Scientific), 10 mM MgCl2) to validate correct folding. .. Agarose gels were run at 24°C for 75 min at 100V, stained with 1xSYBR™ gold nucleic acid stain (S11494, ThermoFisher Scientific)) and visualized with a Gel Doc™ XR+ (Bio Rad).

    Purification:

    Article Title: Evolution of a Designed Protein Assembly Encapsulating its Own RNA Genome
    Article Snippet: .. For synthetic nucleocapsid samples, 20 μL purified synthetic nucleocapsids were treated with 10 μg/mL RNase A (20 °C for 10 minutes), mixed with 4 μL 6x loading dye (NEB B7025S, no SDS), and electrophoresed at 100 volts for 45 minutes. .. Gels were then stained with SYBR gold (Thermo-fischer S11494) for RNA followed by Gelcode (Thermo-fischer 24590) for protein.

    SDS-Gel:

    Article Title: Unprecedentedly efficient CUG initiation of an overlapping reading frame in POLG mRNA yields novel protein POLGARF
    Article Snippet: .. After incubation, the beads were washed twice with 1 ml of PBS supplemented with 1 mM DTT, and bound proteins were eluted by boiling with SDS gel loading buffer. .. The samples were resolved on SDS page and immunoblotted with either anti-FLAG or with custom made anti-POLGARF antibodies.

    Incubation:

    Article Title: Unprecedentedly efficient CUG initiation of an overlapping reading frame in POLG mRNA yields novel protein POLGARF
    Article Snippet: .. After incubation, the beads were washed twice with 1 ml of PBS supplemented with 1 mM DTT, and bound proteins were eluted by boiling with SDS gel loading buffer. .. The samples were resolved on SDS page and immunoblotted with either anti-FLAG or with custom made anti-POLGARF antibodies.

    Polymerase Chain Reaction:

    Article Title: Genome-wide mapping of nucleotide excision repair with XR-seq
    Article Snippet: .. Terminal transferase (TdT) (NEB, cat. no. M0315S) Kapa HiFi PCR HotStart ReadyMix, (Kapa Biosystems, cat. no. KK 2602) Gel-loading dye (purple, with SDS; NEB, cat. no. B7024S) Gel-loading dye (purple, no SDS; NEB, cat. no. B7025S) Acrylamide (Thermo Fisher, cat. no. 15512–023) CAUTION Acrylamide is an irritant and is toxic and carcinogenic. .. Handle it in a fume hood and wear protective clothing, gloves and safety goggles.

    Article Title: A novel isoform of ACE2 is expressed in human nasal and bronchial respiratory epithelia and is upregulated in response to RNA respiratory virus infection
    Article Snippet: .. Gels were run for 75 minutes at 90 V. PCR products were loaded with 6X purple gel loading dye (B7025, NEB) and electrophoresed alongside a low molecular weight ladder (range 25 to 766 base pairs, N3233, NEB) or HyperLadder 1 kb (range 200 bp to 10 kb, BIO-33053, Bioline) to determine product sizes. .. Cilia extraction Cilia were extracted from differentiated ALI cultures on 24-Transwell inserts (Costar) following a protocol modified from .

    Molecular Weight:

    Article Title: A novel isoform of ACE2 is expressed in human nasal and bronchial respiratory epithelia and is upregulated in response to RNA respiratory virus infection
    Article Snippet: .. Gels were run for 75 minutes at 90 V. PCR products were loaded with 6X purple gel loading dye (B7025, NEB) and electrophoresed alongside a low molecular weight ladder (range 25 to 766 base pairs, N3233, NEB) or HyperLadder 1 kb (range 200 bp to 10 kb, BIO-33053, Bioline) to determine product sizes. .. Cilia extraction Cilia were extracted from differentiated ALI cultures on 24-Transwell inserts (Costar) following a protocol modified from .

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  • 99
    New England Biolabs gel loading dye purple 6x no sds
    Gel Loading Dye Purple 6x No Sds, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gel loading dye purple 6x no sds/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gel loading dye purple 6x no sds - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

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