gel loading dye orange 6x  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Name:
    Gel Loading Dye Orange 6X
    Description:
    Gel Loading Dye Orange 6X 4 0 ml
    Catalog Number:
    b7022s
    Price:
    49
    Size:
    4 0 ml
    Category:
    Electrophoresis Tracking Dyes
    Buy from Supplier


    Structured Review

    New England Biolabs gel loading dye orange 6x
    Gel Loading Dye Orange 6X
    Gel Loading Dye Orange 6X 4 0 ml
    https://www.bioz.com/result/gel loading dye orange 6x/product/New England Biolabs
    Average 94 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    gel loading dye orange 6x - by Bioz Stars, 2020-09
    94/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: CRISPR/Cas9-Mediated Mutagenesis of Human Pluripotent Stem Cells in Defined Xeno-free E8 Medium
    Article Snippet: .. T7 Endonuclease I (New England Biolabs, cat. no. M0302S) DNeasy Blood and Tissue Kit (Qiagen, cat. no. 69506) NEBuffer 2 10X (New England Biolabs, cat. no. B7002S) 1X TAE (40mM Tris acetate, 1mM EDTA) UltraPure Agarose (Invitrogen, cat. no. 16500) Ethidium Bromide, 1% Solution (Fisher Scientific, cat. no. BP1302-10) Orange Gel Loading Dye 6X (New England Biolabs, cat. no. B7022S) 10X Lysis Buffer (0.45% NP-40, 0.45% Tween 20 in 10mM Tris pH8.0), store at 4°C NP-40; IGEPAL CA-630 (Sigma-Aldrich, cat. no. I8896) Tween 20 (Fisher Scientific, cat. no. BP337) Tris Base (Fisher Scientific, cat. no. BP154-1) Proteinase K, recombinant, PCR grade (Roche, cat. no. 3115879001) Adhesive PCR plate seals (ThermoFisher Scientific, cat. no. AB-0558) Zero Blunt TOPO PCR Cloning Kit (Life Technologies, cat. no. 450245) .. 3.1 Generation of iCas9 hPSCs 3.2 hPSC culture and passaging in E8 conditions 3.2.1 Vitronectin preparation 3.2.2 hPSC maintenance and passaging in E8 with EDTA dissociation 3.3 Design and production of sgRNAs 3.4 SgRNA transfection of hPSCs in E8 to create hPSC mutant lines 3.5 T7 Endonuclease I (T7E1) Assay to determine frequency of indel mutagenesis.

    Binding Assay:

    Article Title: Chromatin Immunoprecipitation with Fixed Animal Tissues and Preparation for High-Throughput Sequencing
    Article Snippet: .. Bead binding buffer < R > Cell lysis buffer for ChIP-Seq < R > ChIP-Seq dilution buffer < R > ChIP-Seq elution buffer < R > ChIP-Seq nuclear lysis buffer (see Step 24) < R > ChIP-Seq wash buffer < R > DNA loading dye (e.g., Orange Loading Dye [NEB B7022S]) Standard DNA loading dye recipes call for bromophenol blue, which migrates at the same rate as the desired chromatin fragments. ..

    Agarose Gel Electrophoresis:

    Article Title: Systematic evaluation and optimization of the experimental steps in RNA G-quadruplex structure sequencing
    Article Snippet: .. PCR samples were added with 6X orange loading dye (NEB, B7022S) and then resolved on a 1.8% TAE agarose gel at 120 V, 55 min with 4 μl Quick-load Purple LMW marker (NEB, N0557S) included. .. The 150–400 nt region of each PCR product was cut and purified by using the Zymo DNA agarose gel extraction kit (Zymo, D4008) following manufacturer’s protocol.

    Nucleic Acid Electrophoresis:

    Article Title: Protamine loops DNA in multiple steps.
    Article Snippet: .. To purify the DNA, we performed gel electrophoresis using orange loading dye (B7022S, New England Biolabs) and standard protocols for short DNA molecules (35), then purified the DNA via gel extraction (QIAquick Gel Extraction Kit, Qiagen). ..

    Purification:

    Article Title: Protamine loops DNA in multiple steps.
    Article Snippet: .. To purify the DNA, we performed gel electrophoresis using orange loading dye (B7022S, New England Biolabs) and standard protocols for short DNA molecules (35), then purified the DNA via gel extraction (QIAquick Gel Extraction Kit, Qiagen). ..

    Electrophoresis:

    Article Title: DNA Fragmentation Simulation Method (FSM) and Fragment Size Matching Improve aCGH Performance of FFPE Tissues
    Article Snippet: .. Sample aliquots were combined with 4 µl Orange (6×) Gel Loading Dye (New England Biolabs, cat. no. B7022S, Ipswich, MA) and loaded on 1.5% agarose 1X TBE gels prior to electrophoresis at 100–120 V. Gels were stained with GelRed Nucleic Acid Stain (Phenix Research Products, cat. no. RGB-4103, Candler, NC). .. Utilizing open-source ImageJ analysis software (U. S. National Institutes of Health, Bethesda, MD), the mode fragment size of each aliquot was approximated by referencing the maximum intensity of each smear with the bands of a 100 bp DNA Ladder (New England Biolabs, cat. no. N3231S).

    Marker:

    Article Title: Systematic evaluation and optimization of the experimental steps in RNA G-quadruplex structure sequencing
    Article Snippet: .. PCR samples were added with 6X orange loading dye (NEB, B7022S) and then resolved on a 1.8% TAE agarose gel at 120 V, 55 min with 4 μl Quick-load Purple LMW marker (NEB, N0557S) included. .. The 150–400 nt region of each PCR product was cut and purified by using the Zymo DNA agarose gel extraction kit (Zymo, D4008) following manufacturer’s protocol.

    Generated:

    Article Title: GRID-seq for comprehensive analysis of global RNA-chromatin interactions.
    Article Snippet: .. Chromatin in higher eukaryotic nuclei is extensively bound by various RNA species. ..

    Lysis:

    Article Title: Chromatin Immunoprecipitation with Fixed Animal Tissues and Preparation for High-Throughput Sequencing
    Article Snippet: .. Bead binding buffer < R > Cell lysis buffer for ChIP-Seq < R > ChIP-Seq dilution buffer < R > ChIP-Seq elution buffer < R > ChIP-Seq nuclear lysis buffer (see Step 24) < R > ChIP-Seq wash buffer < R > DNA loading dye (e.g., Orange Loading Dye [NEB B7022S]) Standard DNA loading dye recipes call for bromophenol blue, which migrates at the same rate as the desired chromatin fragments. ..

    Article Title: CRISPR/Cas9-Mediated Mutagenesis of Human Pluripotent Stem Cells in Defined Xeno-free E8 Medium
    Article Snippet: .. T7 Endonuclease I (New England Biolabs, cat. no. M0302S) DNeasy Blood and Tissue Kit (Qiagen, cat. no. 69506) NEBuffer 2 10X (New England Biolabs, cat. no. B7002S) 1X TAE (40mM Tris acetate, 1mM EDTA) UltraPure Agarose (Invitrogen, cat. no. 16500) Ethidium Bromide, 1% Solution (Fisher Scientific, cat. no. BP1302-10) Orange Gel Loading Dye 6X (New England Biolabs, cat. no. B7022S) 10X Lysis Buffer (0.45% NP-40, 0.45% Tween 20 in 10mM Tris pH8.0), store at 4°C NP-40; IGEPAL CA-630 (Sigma-Aldrich, cat. no. I8896) Tween 20 (Fisher Scientific, cat. no. BP337) Tris Base (Fisher Scientific, cat. no. BP154-1) Proteinase K, recombinant, PCR grade (Roche, cat. no. 3115879001) Adhesive PCR plate seals (ThermoFisher Scientific, cat. no. AB-0558) Zero Blunt TOPO PCR Cloning Kit (Life Technologies, cat. no. 450245) .. 3.1 Generation of iCas9 hPSCs 3.2 hPSC culture and passaging in E8 conditions 3.2.1 Vitronectin preparation 3.2.2 hPSC maintenance and passaging in E8 with EDTA dissociation 3.3 Design and production of sgRNAs 3.4 SgRNA transfection of hPSCs in E8 to create hPSC mutant lines 3.5 T7 Endonuclease I (T7E1) Assay to determine frequency of indel mutagenesis.

    Polymerase Chain Reaction:

    Article Title: Systematic evaluation and optimization of the experimental steps in RNA G-quadruplex structure sequencing
    Article Snippet: .. PCR samples were added with 6X orange loading dye (NEB, B7022S) and then resolved on a 1.8% TAE agarose gel at 120 V, 55 min with 4 μl Quick-load Purple LMW marker (NEB, N0557S) included. .. The 150–400 nt region of each PCR product was cut and purified by using the Zymo DNA agarose gel extraction kit (Zymo, D4008) following manufacturer’s protocol.

    Article Title: CRISPR/Cas9-Mediated Mutagenesis of Human Pluripotent Stem Cells in Defined Xeno-free E8 Medium
    Article Snippet: .. T7 Endonuclease I (New England Biolabs, cat. no. M0302S) DNeasy Blood and Tissue Kit (Qiagen, cat. no. 69506) NEBuffer 2 10X (New England Biolabs, cat. no. B7002S) 1X TAE (40mM Tris acetate, 1mM EDTA) UltraPure Agarose (Invitrogen, cat. no. 16500) Ethidium Bromide, 1% Solution (Fisher Scientific, cat. no. BP1302-10) Orange Gel Loading Dye 6X (New England Biolabs, cat. no. B7022S) 10X Lysis Buffer (0.45% NP-40, 0.45% Tween 20 in 10mM Tris pH8.0), store at 4°C NP-40; IGEPAL CA-630 (Sigma-Aldrich, cat. no. I8896) Tween 20 (Fisher Scientific, cat. no. BP337) Tris Base (Fisher Scientific, cat. no. BP154-1) Proteinase K, recombinant, PCR grade (Roche, cat. no. 3115879001) Adhesive PCR plate seals (ThermoFisher Scientific, cat. no. AB-0558) Zero Blunt TOPO PCR Cloning Kit (Life Technologies, cat. no. 450245) .. 3.1 Generation of iCas9 hPSCs 3.2 hPSC culture and passaging in E8 conditions 3.2.1 Vitronectin preparation 3.2.2 hPSC maintenance and passaging in E8 with EDTA dissociation 3.3 Design and production of sgRNAs 3.4 SgRNA transfection of hPSCs in E8 to create hPSC mutant lines 3.5 T7 Endonuclease I (T7E1) Assay to determine frequency of indel mutagenesis.

    Gel Extraction:

    Article Title: Protamine loops DNA in multiple steps.
    Article Snippet: .. To purify the DNA, we performed gel electrophoresis using orange loading dye (B7022S, New England Biolabs) and standard protocols for short DNA molecules (35), then purified the DNA via gel extraction (QIAquick Gel Extraction Kit, Qiagen). ..

    Recombinant:

    Article Title: CRISPR/Cas9-Mediated Mutagenesis of Human Pluripotent Stem Cells in Defined Xeno-free E8 Medium
    Article Snippet: .. T7 Endonuclease I (New England Biolabs, cat. no. M0302S) DNeasy Blood and Tissue Kit (Qiagen, cat. no. 69506) NEBuffer 2 10X (New England Biolabs, cat. no. B7002S) 1X TAE (40mM Tris acetate, 1mM EDTA) UltraPure Agarose (Invitrogen, cat. no. 16500) Ethidium Bromide, 1% Solution (Fisher Scientific, cat. no. BP1302-10) Orange Gel Loading Dye 6X (New England Biolabs, cat. no. B7022S) 10X Lysis Buffer (0.45% NP-40, 0.45% Tween 20 in 10mM Tris pH8.0), store at 4°C NP-40; IGEPAL CA-630 (Sigma-Aldrich, cat. no. I8896) Tween 20 (Fisher Scientific, cat. no. BP337) Tris Base (Fisher Scientific, cat. no. BP154-1) Proteinase K, recombinant, PCR grade (Roche, cat. no. 3115879001) Adhesive PCR plate seals (ThermoFisher Scientific, cat. no. AB-0558) Zero Blunt TOPO PCR Cloning Kit (Life Technologies, cat. no. 450245) .. 3.1 Generation of iCas9 hPSCs 3.2 hPSC culture and passaging in E8 conditions 3.2.1 Vitronectin preparation 3.2.2 hPSC maintenance and passaging in E8 with EDTA dissociation 3.3 Design and production of sgRNAs 3.4 SgRNA transfection of hPSCs in E8 to create hPSC mutant lines 3.5 T7 Endonuclease I (T7E1) Assay to determine frequency of indel mutagenesis.

    ChIP-sequencing:

    Article Title: Chromatin Immunoprecipitation with Fixed Animal Tissues and Preparation for High-Throughput Sequencing
    Article Snippet: .. Bead binding buffer < R > Cell lysis buffer for ChIP-Seq < R > ChIP-Seq dilution buffer < R > ChIP-Seq elution buffer < R > ChIP-Seq nuclear lysis buffer (see Step 24) < R > ChIP-Seq wash buffer < R > DNA loading dye (e.g., Orange Loading Dye [NEB B7022S]) Standard DNA loading dye recipes call for bromophenol blue, which migrates at the same rate as the desired chromatin fragments. ..

    Staining:

    Article Title: DNA Fragmentation Simulation Method (FSM) and Fragment Size Matching Improve aCGH Performance of FFPE Tissues
    Article Snippet: .. Sample aliquots were combined with 4 µl Orange (6×) Gel Loading Dye (New England Biolabs, cat. no. B7022S, Ipswich, MA) and loaded on 1.5% agarose 1X TBE gels prior to electrophoresis at 100–120 V. Gels were stained with GelRed Nucleic Acid Stain (Phenix Research Products, cat. no. RGB-4103, Candler, NC). .. Utilizing open-source ImageJ analysis software (U. S. National Institutes of Health, Bethesda, MD), the mode fragment size of each aliquot was approximated by referencing the maximum intensity of each smear with the bands of a 100 bp DNA Ladder (New England Biolabs, cat. no. N3231S).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    New England Biolabs gel loading dye orange 6x
    Gel Loading Dye Orange 6x, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gel loading dye orange 6x/product/New England Biolabs
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    gel loading dye orange 6x - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    Image Search Results