nebuffer 3  (New England Biolabs)


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  • 99
    Name:
    NEBuffer 3
    Description:
    NEBuffer 3 5 0 ml
    Catalog Number:
    b7003s
    Price:
    24
    Size:
    5 0 ml
    Category:
    Buffers
    Buy from Supplier


    Structured Review

    New England Biolabs nebuffer 3
    NEBuffer 3
    NEBuffer 3 5 0 ml
    https://www.bioz.com/result/nebuffer 3/product/New England Biolabs
    Average 99 stars, based on 110 article reviews
    Price from $9.99 to $1999.99
    nebuffer 3 - by Bioz Stars, 2020-08
    99/100 stars

    Images

    1) Product Images from "Host-regulated Hepatitis B Virus Capsid Assembly in a Mammalian Cell-free System"

    Article Title: Host-regulated Hepatitis B Virus Capsid Assembly in a Mammalian Cell-free System

    Journal: Bio-protocol

    doi: 10.21769/BioProtoc.2813

    HBV capsid assembly in RRL under different conditions The WT HBc proteins were translated in RRL, and the translation reaction mixtures were resolved by agarose gel (1%) electrophoresis (A) or SDS-PAGE (12.5%) (B) without any further treatment (Input) (lane 1) or were treated with NEBuffer 3 alone overnight at 37 °C (Mock) (lane 2), with NEBuffer 3 plus CIAP overnight at 37 °C (CIAP) (lane 3), with NEBuffer 3 plus CIAP overnight at 37 °C followed by RNase treatment for one additional hour (CIAP + RNase) (lane 4), with RNase for 1 h followed by NEBuffer 3 plus CIAP overnight at 37 °C (RNase + CIAP) (lane 5), or with the mixture of phosphatase inhibitors overnight at 37 °C (PPI) (lane 6). All lanes contained 2 µl translation products. 35 )
    Figure Legend Snippet: HBV capsid assembly in RRL under different conditions The WT HBc proteins were translated in RRL, and the translation reaction mixtures were resolved by agarose gel (1%) electrophoresis (A) or SDS-PAGE (12.5%) (B) without any further treatment (Input) (lane 1) or were treated with NEBuffer 3 alone overnight at 37 °C (Mock) (lane 2), with NEBuffer 3 plus CIAP overnight at 37 °C (CIAP) (lane 3), with NEBuffer 3 plus CIAP overnight at 37 °C followed by RNase treatment for one additional hour (CIAP + RNase) (lane 4), with RNase for 1 h followed by NEBuffer 3 plus CIAP overnight at 37 °C (RNase + CIAP) (lane 5), or with the mixture of phosphatase inhibitors overnight at 37 °C (PPI) (lane 6). All lanes contained 2 µl translation products. 35 )

    Techniques Used: Agarose Gel Electrophoresis, Electrophoresis, SDS Page

    Related Articles

    Protease Inhibitor:

    Article Title: Host-regulated Hepatitis B Virus Capsid Assembly in a Mammalian Cell-free System
    Article Snippet: .. Pipette tips (Denville Scientific, catalog numbers: P1096-FR, P1121, P1122, P1126) 1.5 ml microcentrifuge tube (Denville Scientific, catalog number: C2170) Gloves and lab coat (Denville Scientific, catalog number: G4162; Medline Industries, catalog number: 83044QHW) Proteinase K, RNA grade (Thermo Fisher Scientific, Invitrogen™, catalog number: 25530049) Sodium dodecyl sulfate (SDS) (Sigma-Aldrich, catalog number: L4509-1KG) Phenol solution (Sigma-Aldrich, catalog number: P4557) Chloroform (Fisher Scientific, catalog number: BP1145-1) Sodium acetate (Sigma-Aldrich, catalog number: S2889-1KG) Ethyl alcohol (EtOH) (AmericanBio, catalog number: AB00515-00100) UltraPure™ DNase/RNase-Free Distilled Water (Thermo Fisher Scientific, Invitrogen™, catalog number: 10977015) Agarose (Thermo Fisher Scientific, Invitrogen™, catalog number: 15510027) TNT® Coupled Rabbit Reticulocyte Lysate (RRL) (Promega, catalog number: L4610) EasyTag™ L-[35 S]-Methionine (PerkinElmer, catalog number: NEG709A001MC) RNasin® Plus Ribonuclease Inhibitor (Promega, catalog number: N2611) RNaseZap™ RNase Decontamination Solution (Thermo Fisher Scientific, Invitrogen™, catalog number: AM9780) RNase A, DNase and protease-free (Thermo Fisher Scientific, Thermo Scientific™, catalog number: EN0531) NEBuffer 3 (New England Biolabs, catalog number: B7003S) Alkaline Phosphatase, Calf Intestinal (CIAP) (New England Biolabs, catalog number: M0290S) Tris Base (Fisher Scientific, catalog number: BP152-10) Ethylenediaminetetraacetic acid, EDTA (Sigma-Aldrich, catalog number: E5134) Sodium fluoride (Sigma-Aldrich, catalog number: S7920) Sodium pyrophosphate tetrabasic decahydrate (Sigma-Aldrich, catalog number: S6422) β-Glycerophosphate (Sigma-Aldrich, catalog number: G6251) Sodium orthovanadate (Sigma-Aldrich, catalog number: S6508) cOmplete™, EDTA-free Protease Inhibitor Cocktail (Roche Diagnostics, catalog number: 04693132001) TE buffer (see Recipes) 10× phosphatase inhibitors (PPI) (see Recipes) 25× protease inhibitor (see Recipes) .. Pipettes (Gilson, P1000, P200, P20, P2) Fume hood ( e.g. , Protector Xstream Laboratory Hood, Labconco) 30 °C/37 °C Oven (SciGene, Robbins Scientific, model: Model 400) Microcentrifuge (Fisher Scientific, Fisherbrand™, model: accuSpin™ Micro 17) Spectrophotometer (Thermo Fisher Scientific, Thermo Scientific™, model: NanoDrop™1000)

    Restriction Assay:

    Article Title: Transcription Blockage by Bulky End-Termini at Single-Strand Breaks in the DNA Template: Differential Effects of 5′ and 3′ Adducts
    Article Snippet: .. The restriction assay was carried out in a total volume of 50 μL containing 0.83 pmol of radiolabeled annealed insert, 1X NEBuffer 3 (NEB) and 1X BSA (NEB) with or without 20 U of SalI. .. EDTA was added to a concentration of 20 mM and the restriction enzyme was inactivated by heating at 65°C for 20min.

    Transferring:

    Article Title: Host-regulated Hepatitis B Virus Capsid Assembly in a Mammalian Cell-free System
    Article Snippet: .. Pipette tips (Denville Scientific, catalog numbers: P1096-FR, P1121, P1122, P1126) 1.5 ml microcentrifuge tube (Denville Scientific, catalog number: C2170) Gloves and lab coat (Denville Scientific, catalog number: G4162; Medline Industries, catalog number: 83044QHW) Proteinase K, RNA grade (Thermo Fisher Scientific, Invitrogen™, catalog number: 25530049) Sodium dodecyl sulfate (SDS) (Sigma-Aldrich, catalog number: L4509-1KG) Phenol solution (Sigma-Aldrich, catalog number: P4557) Chloroform (Fisher Scientific, catalog number: BP1145-1) Sodium acetate (Sigma-Aldrich, catalog number: S2889-1KG) Ethyl alcohol (EtOH) (AmericanBio, catalog number: AB00515-00100) UltraPure™ DNase/RNase-Free Distilled Water (Thermo Fisher Scientific, Invitrogen™, catalog number: 10977015) Agarose (Thermo Fisher Scientific, Invitrogen™, catalog number: 15510027) TNT® Coupled Rabbit Reticulocyte Lysate (RRL) (Promega, catalog number: L4610) EasyTag™ L-[35 S]-Methionine (PerkinElmer, catalog number: NEG709A001MC) RNasin® Plus Ribonuclease Inhibitor (Promega, catalog number: N2611) RNaseZap™ RNase Decontamination Solution (Thermo Fisher Scientific, Invitrogen™, catalog number: AM9780) RNase A, DNase and protease-free (Thermo Fisher Scientific, Thermo Scientific™, catalog number: EN0531) NEBuffer 3 (New England Biolabs, catalog number: B7003S) Alkaline Phosphatase, Calf Intestinal (CIAP) (New England Biolabs, catalog number: M0290S) Tris Base (Fisher Scientific, catalog number: BP152-10) Ethylenediaminetetraacetic acid, EDTA (Sigma-Aldrich, catalog number: E5134) Sodium fluoride (Sigma-Aldrich, catalog number: S7920) Sodium pyrophosphate tetrabasic decahydrate (Sigma-Aldrich, catalog number: S6422) β-Glycerophosphate (Sigma-Aldrich, catalog number: G6251) Sodium orthovanadate (Sigma-Aldrich, catalog number: S6508) cOmplete™, EDTA-free Protease Inhibitor Cocktail (Roche Diagnostics, catalog number: 04693132001) TE buffer (see Recipes) 10× phosphatase inhibitors (PPI) (see Recipes) 25× protease inhibitor (see Recipes) .. Pipettes (Gilson, P1000, P200, P20, P2) Fume hood ( e.g. , Protector Xstream Laboratory Hood, Labconco) 30 °C/37 °C Oven (SciGene, Robbins Scientific, model: Model 400) Microcentrifuge (Fisher Scientific, Fisherbrand™, model: accuSpin™ Micro 17) Spectrophotometer (Thermo Fisher Scientific, Thermo Scientific™, model: NanoDrop™1000)

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  • 99
    New England Biolabs nebuffer 3
    HBV capsid assembly in RRL under different conditions The WT HBc proteins were translated in RRL, and the translation reaction mixtures were resolved by agarose gel (1%) electrophoresis (A) or SDS-PAGE (12.5%) (B) without any further treatment (Input) (lane 1) or were treated with <t>NEBuffer</t> 3 alone overnight at 37 °C (Mock) (lane 2), with NEBuffer 3 plus CIAP overnight at 37 °C (CIAP) (lane 3), with NEBuffer 3 plus CIAP overnight at 37 °C followed by RNase treatment for one additional hour (CIAP + RNase) (lane 4), with RNase for 1 h followed by NEBuffer 3 plus CIAP overnight at 37 °C (RNase + CIAP) (lane 5), or with the mixture of phosphatase inhibitors overnight at 37 °C (PPI) (lane 6). All lanes contained 2 µl translation products. 35 )
    Nebuffer 3, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebuffer 3/product/New England Biolabs
    Average 99 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    nebuffer 3 - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    HBV capsid assembly in RRL under different conditions The WT HBc proteins were translated in RRL, and the translation reaction mixtures were resolved by agarose gel (1%) electrophoresis (A) or SDS-PAGE (12.5%) (B) without any further treatment (Input) (lane 1) or were treated with NEBuffer 3 alone overnight at 37 °C (Mock) (lane 2), with NEBuffer 3 plus CIAP overnight at 37 °C (CIAP) (lane 3), with NEBuffer 3 plus CIAP overnight at 37 °C followed by RNase treatment for one additional hour (CIAP + RNase) (lane 4), with RNase for 1 h followed by NEBuffer 3 plus CIAP overnight at 37 °C (RNase + CIAP) (lane 5), or with the mixture of phosphatase inhibitors overnight at 37 °C (PPI) (lane 6). All lanes contained 2 µl translation products. 35 )

    Journal: Bio-protocol

    Article Title: Host-regulated Hepatitis B Virus Capsid Assembly in a Mammalian Cell-free System

    doi: 10.21769/BioProtoc.2813

    Figure Lengend Snippet: HBV capsid assembly in RRL under different conditions The WT HBc proteins were translated in RRL, and the translation reaction mixtures were resolved by agarose gel (1%) electrophoresis (A) or SDS-PAGE (12.5%) (B) without any further treatment (Input) (lane 1) or were treated with NEBuffer 3 alone overnight at 37 °C (Mock) (lane 2), with NEBuffer 3 plus CIAP overnight at 37 °C (CIAP) (lane 3), with NEBuffer 3 plus CIAP overnight at 37 °C followed by RNase treatment for one additional hour (CIAP + RNase) (lane 4), with RNase for 1 h followed by NEBuffer 3 plus CIAP overnight at 37 °C (RNase + CIAP) (lane 5), or with the mixture of phosphatase inhibitors overnight at 37 °C (PPI) (lane 6). All lanes contained 2 µl translation products. 35 )

    Article Snippet: Pipette tips (Denville Scientific, catalog numbers: P1096-FR, P1121, P1122, P1126) 1.5 ml microcentrifuge tube (Denville Scientific, catalog number: C2170) Gloves and lab coat (Denville Scientific, catalog number: G4162; Medline Industries, catalog number: 83044QHW) Proteinase K, RNA grade (Thermo Fisher Scientific, Invitrogen™, catalog number: 25530049) Sodium dodecyl sulfate (SDS) (Sigma-Aldrich, catalog number: L4509-1KG) Phenol solution (Sigma-Aldrich, catalog number: P4557) Chloroform (Fisher Scientific, catalog number: BP1145-1) Sodium acetate (Sigma-Aldrich, catalog number: S2889-1KG) Ethyl alcohol (EtOH) (AmericanBio, catalog number: AB00515-00100) UltraPure™ DNase/RNase-Free Distilled Water (Thermo Fisher Scientific, Invitrogen™, catalog number: 10977015) Agarose (Thermo Fisher Scientific, Invitrogen™, catalog number: 15510027) TNT® Coupled Rabbit Reticulocyte Lysate (RRL) (Promega, catalog number: L4610) EasyTag™ L-[35 S]-Methionine (PerkinElmer, catalog number: NEG709A001MC) RNasin® Plus Ribonuclease Inhibitor (Promega, catalog number: N2611) RNaseZap™ RNase Decontamination Solution (Thermo Fisher Scientific, Invitrogen™, catalog number: AM9780) RNase A, DNase and protease-free (Thermo Fisher Scientific, Thermo Scientific™, catalog number: EN0531) NEBuffer 3 (New England Biolabs, catalog number: B7003S) Alkaline Phosphatase, Calf Intestinal (CIAP) (New England Biolabs, catalog number: M0290S) Tris Base (Fisher Scientific, catalog number: BP152-10) Ethylenediaminetetraacetic acid, EDTA (Sigma-Aldrich, catalog number: E5134) Sodium fluoride (Sigma-Aldrich, catalog number: S7920) Sodium pyrophosphate tetrabasic decahydrate (Sigma-Aldrich, catalog number: S6422) β-Glycerophosphate (Sigma-Aldrich, catalog number: G6251) Sodium orthovanadate (Sigma-Aldrich, catalog number: S6508) cOmplete™, EDTA-free Protease Inhibitor Cocktail (Roche Diagnostics, catalog number: 04693132001) TE buffer (see Recipes) 10× phosphatase inhibitors (PPI) (see Recipes) 25× protease inhibitor (see Recipes)

    Techniques: Agarose Gel Electrophoresis, Electrophoresis, SDS Page