buffer  (New England Biolabs)


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    Name:
    NEBuffer 3
    Description:
    NEBuffer 3 5 0 ml
    Catalog Number:
    B7003S
    Price:
    24
    Category:
    Buffers
    Size:
    5 0 ml
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    Structured Review

    New England Biolabs buffer
    NEBuffer 3
    NEBuffer 3 5 0 ml
    https://www.bioz.com/result/buffer/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    buffer - by Bioz Stars, 2021-06
    99/100 stars

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    Related Articles

    Transferring:

    Article Title: Host-regulated Hepatitis B Virus Capsid Assembly in a Mammalian Cell-free System
    Article Snippet: .. Pipette tips (Denville Scientific, catalog numbers: P1096-FR, P1121, P1122, P1126) 1.5 ml microcentrifuge tube (Denville Scientific, catalog number: C2170) Gloves and lab coat (Denville Scientific, catalog number: G4162; Medline Industries, catalog number: 83044QHW) Proteinase K, RNA grade (Thermo Fisher Scientific, Invitrogen™, catalog number: 25530049) Sodium dodecyl sulfate (SDS) (Sigma-Aldrich, catalog number: L4509-1KG) Phenol solution (Sigma-Aldrich, catalog number: P4557) Chloroform (Fisher Scientific, catalog number: BP1145-1) Sodium acetate (Sigma-Aldrich, catalog number: S2889-1KG) Ethyl alcohol (EtOH) (AmericanBio, catalog number: AB00515-00100) UltraPure™ DNase/RNase-Free Distilled Water (Thermo Fisher Scientific, Invitrogen™, catalog number: 10977015) Agarose (Thermo Fisher Scientific, Invitrogen™, catalog number: 15510027) TNT® Coupled Rabbit Reticulocyte Lysate (RRL) (Promega, catalog number: L4610) EasyTag™ L-[35 S]-Methionine (PerkinElmer, catalog number: NEG709A001MC) RNasin® Plus Ribonuclease Inhibitor (Promega, catalog number: N2611) RNaseZap™ RNase Decontamination Solution (Thermo Fisher Scientific, Invitrogen™, catalog number: AM9780) RNase A, DNase and protease-free (Thermo Fisher Scientific, Thermo Scientific™, catalog number: EN0531) NEBuffer 3 (New England Biolabs, catalog number: B7003S) Alkaline Phosphatase, Calf Intestinal (CIAP) (New England Biolabs, catalog number: M0290S) Tris Base (Fisher Scientific, catalog number: BP152-10) Ethylenediaminetetraacetic acid, EDTA (Sigma-Aldrich, catalog number: E5134) Sodium fluoride (Sigma-Aldrich, catalog number: S7920) Sodium pyrophosphate tetrabasic decahydrate (Sigma-Aldrich, catalog number: S6422) β-Glycerophosphate (Sigma-Aldrich, catalog number: G6251) Sodium orthovanadate (Sigma-Aldrich, catalog number: S6508) cOmplete™, EDTA-free Protease Inhibitor Cocktail (Roche Diagnostics, catalog number: 04693132001) TE buffer (see Recipes) 10× phosphatase inhibitors (PPI) (see Recipes) 25× protease inhibitor (see Recipes) .. Pipettes (Gilson, P1000, P200, P20, P2) Fume hood ( e.g. , Protector Xstream Laboratory Hood, Labconco) 30 °C/37 °C Oven (SciGene, Robbins Scientific, model: Model 400) Microcentrifuge (Fisher Scientific, Fisherbrand™, model: accuSpin™ Micro 17) Spectrophotometer (Thermo Fisher Scientific, Thermo Scientific™, model: NanoDrop™1000)

    Protease Inhibitor:

    Article Title: Host-regulated Hepatitis B Virus Capsid Assembly in a Mammalian Cell-free System
    Article Snippet: .. Pipette tips (Denville Scientific, catalog numbers: P1096-FR, P1121, P1122, P1126) 1.5 ml microcentrifuge tube (Denville Scientific, catalog number: C2170) Gloves and lab coat (Denville Scientific, catalog number: G4162; Medline Industries, catalog number: 83044QHW) Proteinase K, RNA grade (Thermo Fisher Scientific, Invitrogen™, catalog number: 25530049) Sodium dodecyl sulfate (SDS) (Sigma-Aldrich, catalog number: L4509-1KG) Phenol solution (Sigma-Aldrich, catalog number: P4557) Chloroform (Fisher Scientific, catalog number: BP1145-1) Sodium acetate (Sigma-Aldrich, catalog number: S2889-1KG) Ethyl alcohol (EtOH) (AmericanBio, catalog number: AB00515-00100) UltraPure™ DNase/RNase-Free Distilled Water (Thermo Fisher Scientific, Invitrogen™, catalog number: 10977015) Agarose (Thermo Fisher Scientific, Invitrogen™, catalog number: 15510027) TNT® Coupled Rabbit Reticulocyte Lysate (RRL) (Promega, catalog number: L4610) EasyTag™ L-[35 S]-Methionine (PerkinElmer, catalog number: NEG709A001MC) RNasin® Plus Ribonuclease Inhibitor (Promega, catalog number: N2611) RNaseZap™ RNase Decontamination Solution (Thermo Fisher Scientific, Invitrogen™, catalog number: AM9780) RNase A, DNase and protease-free (Thermo Fisher Scientific, Thermo Scientific™, catalog number: EN0531) NEBuffer 3 (New England Biolabs, catalog number: B7003S) Alkaline Phosphatase, Calf Intestinal (CIAP) (New England Biolabs, catalog number: M0290S) Tris Base (Fisher Scientific, catalog number: BP152-10) Ethylenediaminetetraacetic acid, EDTA (Sigma-Aldrich, catalog number: E5134) Sodium fluoride (Sigma-Aldrich, catalog number: S7920) Sodium pyrophosphate tetrabasic decahydrate (Sigma-Aldrich, catalog number: S6422) β-Glycerophosphate (Sigma-Aldrich, catalog number: G6251) Sodium orthovanadate (Sigma-Aldrich, catalog number: S6508) cOmplete™, EDTA-free Protease Inhibitor Cocktail (Roche Diagnostics, catalog number: 04693132001) TE buffer (see Recipes) 10× phosphatase inhibitors (PPI) (see Recipes) 25× protease inhibitor (see Recipes) .. Pipettes (Gilson, P1000, P200, P20, P2) Fume hood ( e.g. , Protector Xstream Laboratory Hood, Labconco) 30 °C/37 °C Oven (SciGene, Robbins Scientific, model: Model 400) Microcentrifuge (Fisher Scientific, Fisherbrand™, model: accuSpin™ Micro 17) Spectrophotometer (Thermo Fisher Scientific, Thermo Scientific™, model: NanoDrop™1000)

    other:

    Article Title: CRISPR delivery particles for developing therapeutic strategies in metabolic disease
    Article Snippet: Cas9 protein and sgRNA were mixed in NEBuffer 3 (100 mM NaCl, 50 mM Tris-HCl, 10 mM MgCl2, 1 mM DTT, pH 7.9) purchased from New England Biolabs Inc. or PBS at 37 °C for 10 minutes to form nano-size complexes.

    Amplification:

    Article Title: Digital Quantification of miRNA directly in plasma using Integrated Comprehensive Droplet Digital Detection
    Article Snippet: .. EXPAR reagents were introduced separately as part A (NEBuffer 3.1, DNA template, deoxyribonucleotides (dNTPs) and RNase inhibitor) and part B (ThermoPol buffer, DNA polymerase (Vent (exo-)), nicking endonuclease (Nt.BstNBI), EvaGreen and ROX reference dye) to avoid non-specific amplification before introduction of the target miRNA. ..

    Expressing:

    Article Title: CRISPR delivery particles for developing therapeutic strategies in metabolic disease
    Article Snippet: MEGAshortscript T7 Transcription Kit, Lipofectamine® RNAiMAX, Vybrant MTT cell proliferation assay and Platinum™ Taq DNA Polymerase High Fidelity kit were purchased from ThermoFisher Scientific (Waltham, MA, USA). .. BsaI, DraI, T7E1 and NEBuffer 3 were obtained from New England Biolabs Inc. (Ipswich, MA, USA). pUC57-sgRNA expression vector was purchased from Addgene (plasmid # 51132) (Cambridge, MA, USA). ..

    Plasmid Preparation:

    Article Title: CRISPR delivery particles for developing therapeutic strategies in metabolic disease
    Article Snippet: MEGAshortscript T7 Transcription Kit, Lipofectamine® RNAiMAX, Vybrant MTT cell proliferation assay and Platinum™ Taq DNA Polymerase High Fidelity kit were purchased from ThermoFisher Scientific (Waltham, MA, USA). .. BsaI, DraI, T7E1 and NEBuffer 3 were obtained from New England Biolabs Inc. (Ipswich, MA, USA). pUC57-sgRNA expression vector was purchased from Addgene (plasmid # 51132) (Cambridge, MA, USA). ..

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  • 99
    New England Biolabs nebuffer 3
    HBV capsid assembly in RRL under different conditions The WT HBc proteins were translated in RRL, and the translation reaction mixtures were resolved by agarose gel (1%) electrophoresis (A) or SDS-PAGE (12.5%) (B) without any further treatment (Input) (lane 1) or were treated with <t>NEBuffer</t> 3 alone overnight at 37 °C (Mock) (lane 2), with NEBuffer 3 plus CIAP overnight at 37 °C (CIAP) (lane 3), with NEBuffer 3 plus CIAP overnight at 37 °C followed by RNase treatment for one additional hour (CIAP + RNase) (lane 4), with RNase for 1 h followed by NEBuffer 3 plus CIAP overnight at 37 °C (RNase + CIAP) (lane 5), or with the mixture of phosphatase inhibitors overnight at 37 °C (PPI) (lane 6). All lanes contained 2 µl translation products. 35 )
    Nebuffer 3, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebuffer 3/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nebuffer 3 - by Bioz Stars, 2021-06
    99/100 stars
      Buy from Supplier

    Image Search Results


    HBV capsid assembly in RRL under different conditions The WT HBc proteins were translated in RRL, and the translation reaction mixtures were resolved by agarose gel (1%) electrophoresis (A) or SDS-PAGE (12.5%) (B) without any further treatment (Input) (lane 1) or were treated with NEBuffer 3 alone overnight at 37 °C (Mock) (lane 2), with NEBuffer 3 plus CIAP overnight at 37 °C (CIAP) (lane 3), with NEBuffer 3 plus CIAP overnight at 37 °C followed by RNase treatment for one additional hour (CIAP + RNase) (lane 4), with RNase for 1 h followed by NEBuffer 3 plus CIAP overnight at 37 °C (RNase + CIAP) (lane 5), or with the mixture of phosphatase inhibitors overnight at 37 °C (PPI) (lane 6). All lanes contained 2 µl translation products. 35 )

    Journal: Bio-protocol

    Article Title: Host-regulated Hepatitis B Virus Capsid Assembly in a Mammalian Cell-free System

    doi: 10.21769/BioProtoc.2813

    Figure Lengend Snippet: HBV capsid assembly in RRL under different conditions The WT HBc proteins were translated in RRL, and the translation reaction mixtures were resolved by agarose gel (1%) electrophoresis (A) or SDS-PAGE (12.5%) (B) without any further treatment (Input) (lane 1) or were treated with NEBuffer 3 alone overnight at 37 °C (Mock) (lane 2), with NEBuffer 3 plus CIAP overnight at 37 °C (CIAP) (lane 3), with NEBuffer 3 plus CIAP overnight at 37 °C followed by RNase treatment for one additional hour (CIAP + RNase) (lane 4), with RNase for 1 h followed by NEBuffer 3 plus CIAP overnight at 37 °C (RNase + CIAP) (lane 5), or with the mixture of phosphatase inhibitors overnight at 37 °C (PPI) (lane 6). All lanes contained 2 µl translation products. 35 )

    Article Snippet: Pipette tips (Denville Scientific, catalog numbers: P1096-FR, P1121, P1122, P1126) 1.5 ml microcentrifuge tube (Denville Scientific, catalog number: C2170) Gloves and lab coat (Denville Scientific, catalog number: G4162; Medline Industries, catalog number: 83044QHW) Proteinase K, RNA grade (Thermo Fisher Scientific, Invitrogen™, catalog number: 25530049) Sodium dodecyl sulfate (SDS) (Sigma-Aldrich, catalog number: L4509-1KG) Phenol solution (Sigma-Aldrich, catalog number: P4557) Chloroform (Fisher Scientific, catalog number: BP1145-1) Sodium acetate (Sigma-Aldrich, catalog number: S2889-1KG) Ethyl alcohol (EtOH) (AmericanBio, catalog number: AB00515-00100) UltraPure™ DNase/RNase-Free Distilled Water (Thermo Fisher Scientific, Invitrogen™, catalog number: 10977015) Agarose (Thermo Fisher Scientific, Invitrogen™, catalog number: 15510027) TNT® Coupled Rabbit Reticulocyte Lysate (RRL) (Promega, catalog number: L4610) EasyTag™ L-[35 S]-Methionine (PerkinElmer, catalog number: NEG709A001MC) RNasin® Plus Ribonuclease Inhibitor (Promega, catalog number: N2611) RNaseZap™ RNase Decontamination Solution (Thermo Fisher Scientific, Invitrogen™, catalog number: AM9780) RNase A, DNase and protease-free (Thermo Fisher Scientific, Thermo Scientific™, catalog number: EN0531) NEBuffer 3 (New England Biolabs, catalog number: B7003S) Alkaline Phosphatase, Calf Intestinal (CIAP) (New England Biolabs, catalog number: M0290S) Tris Base (Fisher Scientific, catalog number: BP152-10) Ethylenediaminetetraacetic acid, EDTA (Sigma-Aldrich, catalog number: E5134) Sodium fluoride (Sigma-Aldrich, catalog number: S7920) Sodium pyrophosphate tetrabasic decahydrate (Sigma-Aldrich, catalog number: S6422) β-Glycerophosphate (Sigma-Aldrich, catalog number: G6251) Sodium orthovanadate (Sigma-Aldrich, catalog number: S6508) cOmplete™, EDTA-free Protease Inhibitor Cocktail (Roche Diagnostics, catalog number: 04693132001) TE buffer (see Recipes) 10× phosphatase inhibitors (PPI) (see Recipes) 25× protease inhibitor (see Recipes)

    Techniques: Agarose Gel Electrophoresis, Electrophoresis, SDS Page

    Efficient gene deletion achieved following the treatment with CriPs targeting Gfp in primary GFP pre-adipocytes isolated from GFP mice. (A, C) Flow cytometry data of primary GFP pre-adipocytes treated with CriPs formulated in (A) NEBuffer 3 or (C) PBS. Cas9-sgRNA: 100 nM. (B, D) Different concentrations of EP with 100 nM of Cas9-sgRNA formulated in (B) NEBuffer 3 or (D) PBS. (E) Percent Indels measurements by T7E1 assay in genomic DNA isolated from primary GFP pre-adipocytes. Uncut: 401 bp, Cut: 288 bp + 113 bp.

    Journal: bioRxiv

    Article Title: CRISPR delivery particles for developing therapeutic strategies in metabolic disease

    doi: 10.1101/352799

    Figure Lengend Snippet: Efficient gene deletion achieved following the treatment with CriPs targeting Gfp in primary GFP pre-adipocytes isolated from GFP mice. (A, C) Flow cytometry data of primary GFP pre-adipocytes treated with CriPs formulated in (A) NEBuffer 3 or (C) PBS. Cas9-sgRNA: 100 nM. (B, D) Different concentrations of EP with 100 nM of Cas9-sgRNA formulated in (B) NEBuffer 3 or (D) PBS. (E) Percent Indels measurements by T7E1 assay in genomic DNA isolated from primary GFP pre-adipocytes. Uncut: 401 bp, Cut: 288 bp + 113 bp.

    Article Snippet: To optimize the EP dose that causes the maximum gene deletion efficiency of Gfp by CriPs formulated in NEBuffer 3 in the primary GFP pre-adipocytes, the relationship between EP dose and response was determined.

    Techniques: Isolation, Mouse Assay, Flow Cytometry