nebuffer ii  (New England Biolabs)


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  • 99
    Name:
    NEBuffer 1
    Description:
    NEBuffer 1 5 0 ml
    Catalog Number:
    b7001s
    Price:
    24
    Size:
    5 0 ml
    Category:
    Buffers
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    Structured Review

    New England Biolabs nebuffer ii
    NEBuffer 1
    NEBuffer 1 5 0 ml
    https://www.bioz.com/result/nebuffer ii/product/New England Biolabs
    Average 99 stars, based on 4814 article reviews
    Price from $9.99 to $1999.99
    nebuffer ii - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Transduction:

    Article Title: Dissecting super-enhancer hierarchy based on chromatin interactions
    Article Snippet: .. The annealed oligos were cloned into pLV-hU6-sgRNA-hUbC-dCas9-KRAB-T2a-Puro vector (Addgene ID: 71236) using a Golden Gate Assembly strategy including: 100 ng of circular pLV plasmid, 0.2 μM annealed oligos, 2.1 buffer (1×) (New England Biolabs), 20 U of BsmBI restriction enzyme, 0.2 mM ATP, 0.1 mg/ml BSA, and 750 U of T4 DNA ligase (New England Biolabs) with the cycling parameters of 20 cycles of 37 °C for 5 min, 20 °C for 5 min; followed by 80 °C incubation for 20 min. Then K562 cells were transduced with lentivirus to stably express dCa s9-KRAB and sgRNA. .. Briefly, sequence-specific sgRNAs for site-specific interference of genomic targets were designed following described guidelines, and sequences were selected to minimize off-target effect based on publicly available filtering tools ( http://crispr.mit.edu/ ).

    Clone Assay:

    Article Title: Dissecting super-enhancer hierarchy based on chromatin interactions
    Article Snippet: .. The annealed oligos were cloned into pLV-hU6-sgRNA-hUbC-dCas9-KRAB-T2a-Puro vector (Addgene ID: 71236) using a Golden Gate Assembly strategy including: 100 ng of circular pLV plasmid, 0.2 μM annealed oligos, 2.1 buffer (1×) (New England Biolabs), 20 U of BsmBI restriction enzyme, 0.2 mM ATP, 0.1 mg/ml BSA, and 750 U of T4 DNA ligase (New England Biolabs) with the cycling parameters of 20 cycles of 37 °C for 5 min, 20 °C for 5 min; followed by 80 °C incubation for 20 min. Then K562 cells were transduced with lentivirus to stably express dCa s9-KRAB and sgRNA. .. Briefly, sequence-specific sgRNAs for site-specific interference of genomic targets were designed following described guidelines, and sequences were selected to minimize off-target effect based on publicly available filtering tools ( http://crispr.mit.edu/ ).

    Stable Transfection:

    Article Title: Dissecting super-enhancer hierarchy based on chromatin interactions
    Article Snippet: .. The annealed oligos were cloned into pLV-hU6-sgRNA-hUbC-dCas9-KRAB-T2a-Puro vector (Addgene ID: 71236) using a Golden Gate Assembly strategy including: 100 ng of circular pLV plasmid, 0.2 μM annealed oligos, 2.1 buffer (1×) (New England Biolabs), 20 U of BsmBI restriction enzyme, 0.2 mM ATP, 0.1 mg/ml BSA, and 750 U of T4 DNA ligase (New England Biolabs) with the cycling parameters of 20 cycles of 37 °C for 5 min, 20 °C for 5 min; followed by 80 °C incubation for 20 min. Then K562 cells were transduced with lentivirus to stably express dCa s9-KRAB and sgRNA. .. Briefly, sequence-specific sgRNAs for site-specific interference of genomic targets were designed following described guidelines, and sequences were selected to minimize off-target effect based on publicly available filtering tools ( http://crispr.mit.edu/ ).

    Magnetic Beads:

    Article Title: DNA methylation alterations exhibit intra-individual stability and inter-individual heterogeneity in prostate cancer metastases
    Article Snippet: .. After adaptor ligation, the TI fraction was brought to a total volume of 100 uL with water and set aside on ice; the EM fraction was subject to enrichment for methylated DNA fragments using MBD2-MBD polypeptides immobilized on magnetic beads as previously described ( , ) except that the final DNA was eluted in 45 uL of EB1 buffer (0.2X NEBuffer 1 (New England Biolabs, Ipswich, MA), 0.2X BSA (NEB), 0.25X T4 DNA ligase Buffer (NEB) in water) for the DNA previously digested by NspI and 35 uL of EB2 buffer (0.2X NEBuffer3, 0.2X BSA (NEB), 0.25X T4 DNA ligase buffer (NEB) in water) for the DNA previously digested by StyI. ..

    Methylation:

    Article Title: DNA methylation alterations exhibit intra-individual stability and inter-individual heterogeneity in prostate cancer metastases
    Article Snippet: .. After adaptor ligation, the TI fraction was brought to a total volume of 100 uL with water and set aside on ice; the EM fraction was subject to enrichment for methylated DNA fragments using MBD2-MBD polypeptides immobilized on magnetic beads as previously described ( , ) except that the final DNA was eluted in 45 uL of EB1 buffer (0.2X NEBuffer 1 (New England Biolabs, Ipswich, MA), 0.2X BSA (NEB), 0.25X T4 DNA ligase Buffer (NEB) in water) for the DNA previously digested by NspI and 35 uL of EB2 buffer (0.2X NEBuffer3, 0.2X BSA (NEB), 0.25X T4 DNA ligase buffer (NEB) in water) for the DNA previously digested by StyI. ..

    Ligation:

    Article Title: DNA methylation alterations exhibit intra-individual stability and inter-individual heterogeneity in prostate cancer metastases
    Article Snippet: .. After adaptor ligation, the TI fraction was brought to a total volume of 100 uL with water and set aside on ice; the EM fraction was subject to enrichment for methylated DNA fragments using MBD2-MBD polypeptides immobilized on magnetic beads as previously described ( , ) except that the final DNA was eluted in 45 uL of EB1 buffer (0.2X NEBuffer 1 (New England Biolabs, Ipswich, MA), 0.2X BSA (NEB), 0.25X T4 DNA ligase Buffer (NEB) in water) for the DNA previously digested by NspI and 35 uL of EB2 buffer (0.2X NEBuffer3, 0.2X BSA (NEB), 0.25X T4 DNA ligase buffer (NEB) in water) for the DNA previously digested by StyI. ..

    Purification:

    Article Title: Rapid Short-Read Sequencing and Aneuploidy Detection Using MinION Nanopore Technology
    Article Snippet: .. The reaction contained 2.5 µl of NEBuffer II, 1 µl of Klenow fragment (3′→5′ exo-), 16.5 µl of end-repaired purified DNA, and 5 µl of dATP (1 mM). .. Reactions were incubated in a Bio-Rad C1000 Thermal Cycler at 37° for 45 min, purified using 1.8-fold AMPure XP beads, and then eluted in 12 µl of 1/5 Buffer EB.

    Incubation:

    Article Title: Dissecting super-enhancer hierarchy based on chromatin interactions
    Article Snippet: .. The annealed oligos were cloned into pLV-hU6-sgRNA-hUbC-dCas9-KRAB-T2a-Puro vector (Addgene ID: 71236) using a Golden Gate Assembly strategy including: 100 ng of circular pLV plasmid, 0.2 μM annealed oligos, 2.1 buffer (1×) (New England Biolabs), 20 U of BsmBI restriction enzyme, 0.2 mM ATP, 0.1 mg/ml BSA, and 750 U of T4 DNA ligase (New England Biolabs) with the cycling parameters of 20 cycles of 37 °C for 5 min, 20 °C for 5 min; followed by 80 °C incubation for 20 min. Then K562 cells were transduced with lentivirus to stably express dCa s9-KRAB and sgRNA. .. Briefly, sequence-specific sgRNAs for site-specific interference of genomic targets were designed following described guidelines, and sequences were selected to minimize off-target effect based on publicly available filtering tools ( http://crispr.mit.edu/ ).

    Article Title: HIT-scISOseq: High-throughput and High-accuracy Single-cell Full-length Isoform Sequencing for Corneal Epithelium
    Article Snippet: .. To remove residual adapters and unligated DNA fragments, we added 1 μlExonuclease I (NEB), 1 μl Exonuclease III (NEB), and NEBuffer 1 (NEB) to the library and incubated at 37 °C for 1 hour. .. The products were purified using AgencourtAMPure XP Beads and quantified using the Agilent DNA 12000 Kit (Agilent).

    Plasmid Preparation:

    Article Title: Dissecting super-enhancer hierarchy based on chromatin interactions
    Article Snippet: .. The annealed oligos were cloned into pLV-hU6-sgRNA-hUbC-dCas9-KRAB-T2a-Puro vector (Addgene ID: 71236) using a Golden Gate Assembly strategy including: 100 ng of circular pLV plasmid, 0.2 μM annealed oligos, 2.1 buffer (1×) (New England Biolabs), 20 U of BsmBI restriction enzyme, 0.2 mM ATP, 0.1 mg/ml BSA, and 750 U of T4 DNA ligase (New England Biolabs) with the cycling parameters of 20 cycles of 37 °C for 5 min, 20 °C for 5 min; followed by 80 °C incubation for 20 min. Then K562 cells were transduced with lentivirus to stably express dCa s9-KRAB and sgRNA. .. Briefly, sequence-specific sgRNAs for site-specific interference of genomic targets were designed following described guidelines, and sequences were selected to minimize off-target effect based on publicly available filtering tools ( http://crispr.mit.edu/ ).

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  • 99
    New England Biolabs nebuffer ii
    Nebuffer Ii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebuffer ii/product/New England Biolabs
    Average 99 stars, based on 56 article reviews
    Price from $9.99 to $1999.99
    nebuffer ii - by Bioz Stars, 2020-09
    99/100 stars
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