nebuffer1  (New England Biolabs)


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    New England Biolabs nebuffer1
    Nebuffer1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 1 article reviews
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    New England Biolabs nebuffer 1
    Characterization of TkoII restriction endonuclease activities. (A) Predicted TkoII recognition sites in plasmid pBR322 are at positions 1093, 2944 and 4355. (B) TkoII was incubated with pBR322 in 1X <t>NEBuffer</t> 1 supplemented with SAM and 90mer trans-DNA for 30 min at 65°C. Reactions were halted with Proteinase K and loading dye and separated on a 1% agarose gel. Lane 1 is a 1 kb DNA ladder. Lane 2 is pBR322 alone and lanes 3–7 are decreasing amounts of TkoII (10–0.62 nM). (C) TkoII cleaves pUC19 at a single site. (D) pUC19/TkoII cut fragments were analyzed by run off sequencing to determine TkoII cut sites on the top and bottom strands: TTCAAG(N) 10 /(N) 8 .
    Nebuffer 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebuffer 1/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nebuffer 1 - by Bioz Stars, 2022-05
    95/100 stars
      Buy from Supplier

    99
    New England Biolabs nebuffer i scei pack
    Characterization of TkoII restriction endonuclease activities. (A) Predicted TkoII recognition sites in plasmid pBR322 are at positions 1093, 2944 and 4355. (B) TkoII was incubated with pBR322 in 1X <t>NEBuffer</t> 1 supplemented with SAM and 90mer trans-DNA for 30 min at 65°C. Reactions were halted with Proteinase K and loading dye and separated on a 1% agarose gel. Lane 1 is a 1 kb DNA ladder. Lane 2 is pBR322 alone and lanes 3–7 are decreasing amounts of TkoII (10–0.62 nM). (C) TkoII cleaves pUC19 at a single site. (D) pUC19/TkoII cut fragments were analyzed by run off sequencing to determine TkoII cut sites on the top and bottom strands: TTCAAG(N) 10 /(N) 8 .
    Nebuffer I Scei Pack, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebuffer i scei pack/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nebuffer i scei pack - by Bioz Stars, 2022-05
    99/100 stars
      Buy from Supplier

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    Characterization of TkoII restriction endonuclease activities. (A) Predicted TkoII recognition sites in plasmid pBR322 are at positions 1093, 2944 and 4355. (B) TkoII was incubated with pBR322 in 1X NEBuffer 1 supplemented with SAM and 90mer trans-DNA for 30 min at 65°C. Reactions were halted with Proteinase K and loading dye and separated on a 1% agarose gel. Lane 1 is a 1 kb DNA ladder. Lane 2 is pBR322 alone and lanes 3–7 are decreasing amounts of TkoII (10–0.62 nM). (C) TkoII cleaves pUC19 at a single site. (D) pUC19/TkoII cut fragments were analyzed by run off sequencing to determine TkoII cut sites on the top and bottom strands: TTCAAG(N) 10 /(N) 8 .

    Journal: Frontiers in Microbiology

    Article Title: The Hyperthermophilic Restriction-Modification Systems of Thermococcus kodakarensis Protect Genome Integrity

    doi: 10.3389/fmicb.2021.657356

    Figure Lengend Snippet: Characterization of TkoII restriction endonuclease activities. (A) Predicted TkoII recognition sites in plasmid pBR322 are at positions 1093, 2944 and 4355. (B) TkoII was incubated with pBR322 in 1X NEBuffer 1 supplemented with SAM and 90mer trans-DNA for 30 min at 65°C. Reactions were halted with Proteinase K and loading dye and separated on a 1% agarose gel. Lane 1 is a 1 kb DNA ladder. Lane 2 is pBR322 alone and lanes 3–7 are decreasing amounts of TkoII (10–0.62 nM). (C) TkoII cleaves pUC19 at a single site. (D) pUC19/TkoII cut fragments were analyzed by run off sequencing to determine TkoII cut sites on the top and bottom strands: TTCAAG(N) 10 /(N) 8 .

    Article Snippet: TkoI and TkoII REase activity was assayed by mixing TkoI (400 nM) or TkoII (10 nM) with plasmid pBR322 (1 μg) or pUC19 (3 μg), SAM (80 μM), 90mer trans-DNA (0.2 μM) in 1× NEBuffer 3 (100 mM NaCl, 50 mM Tris–HCl, pH 7.9, 10 mM MgCl2 ) for TkoI or NEBuffer 1 (10 mM Bis-Tris–Propane-HCl, pH 7, 10 mM MgCl2, 1 mM DTT) for TkoII in a 50 μL reaction and incubated at 65°C for 30 min. For pBR322 reactions, four two-fold serial dilutions were performed.

    Techniques: Plasmid Preparation, Incubation, Agarose Gel Electrophoresis, Sequencing