second strand synthesis reaction buffer  (New England Biolabs)


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    Name:
    NEBNext Second Strand Synthesis dNTP free Reaction Buffer
    Description:
    NEBNext Second Strand Synthesis dNTP free Reaction Buffer 0 4 ml
    Catalog Number:
    b6117s
    Price:
    30
    Size:
    0 4 ml
    Category:
    Buffers
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    Structured Review

    New England Biolabs second strand synthesis reaction buffer
    NEBNext Second Strand Synthesis dNTP free Reaction Buffer
    NEBNext Second Strand Synthesis dNTP free Reaction Buffer 0 4 ml
    https://www.bioz.com/result/second strand synthesis reaction buffer/product/New England Biolabs
    Average 99 stars, based on 58 article reviews
    Price from $9.99 to $1999.99
    second strand synthesis reaction buffer - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: Biophysical characterization of DNA origami nanostructures reveals inaccessibility to intercalation binding sites
    Article Snippet: .. The individual staples and their complements used in gel electrophoresis experiments were annealed by heating to 95°C and cooling to room temperature at a rate of 1°C per minute to form a 10 µM solution in synthesis buffer. .. 1.3 Purifying Origami Excess staples were removed from the DNA origami using high resolution Sephacryl S-300 media (GE Healthcare) packed into filtration columns [ , ]; Full details of the preparation of the filtration media and packing of the spin columns are given in the supplementary methods.

    Incubation:

    Article Title: LaminA/C regulates epigenetic and chromatin architecture changes upon aging of hematopoietic stem cells
    Article Snippet: .. Second strand synthesis was performed using a custom reaction (20 μl first strand cDNA, 10 μl × 10 thermopol buffer, 1 ml × 100 BSA, 3 ml 10 mM dNTPs, 0.5 μl RnaseH, 1 μl Taq polymerase, 0.1 μl Pfu polymerase, 64.4 ml H2 O) which was incubated in a thermocycler under the following conditions: 37 °C 5 min, 65 °C 1 min, 72 °C 30 min) followed by SPRI purification (beads/DNA ratio 1.8/1). .. Resulting double-stranded DNA was then digested with BpmI for 2 h at 37 °C, purified again with SPRI beads (beads/DNA ratio 1.5/1) and eluted in 13 μl ddH2 ).

    other:

    Article Title: Prokaryotic Single-Cell RNA Sequencing by In Situ Combinatorial Indexing
    Article Snippet: Second strand synthesis was carried out as described and the double-stranded cDNA was used as inp ut for tagmentation.

    Generated:

    Article Title: LINE-1 derepression in senescent cells triggers interferon and inflammaging
    Article Snippet: .. The DNA second strand was then generated by adding a mixture of random primers (Thermo Fisher), second strand synthesis reaction buffer, dNTPs and DNA Pol I (New England Biolabs). .. The reaction was incubated for 4 hrs. at 16˚C and subsequently purified by phenol-chloroform extraction.

    Article Title: Comparative Transcriptome Profile of the Cytoplasmic Male Sterile and Fertile Floral Buds of Radish (Raphanus sativus L.)
    Article Snippet: .. The second-strand cDNA was generated using the Second Strand Synthesis Reaction Buffer and the Second Strand Synthesis Enzyme Mix from NEBNext® Ultra™ RNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA). .. The second-strand cDNA was generated using the Second Strand Synthesis Reaction Buffer and the Second Strand Synthesis Enzyme Mix from NEBNext® Ultra™ RNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA).

    Purification:

    Article Title: LaminA/C regulates epigenetic and chromatin architecture changes upon aging of hematopoietic stem cells
    Article Snippet: .. Second strand synthesis was performed using a custom reaction (20 μl first strand cDNA, 10 μl × 10 thermopol buffer, 1 ml × 100 BSA, 3 ml 10 mM dNTPs, 0.5 μl RnaseH, 1 μl Taq polymerase, 0.1 μl Pfu polymerase, 64.4 ml H2 O) which was incubated in a thermocycler under the following conditions: 37 °C 5 min, 65 °C 1 min, 72 °C 30 min) followed by SPRI purification (beads/DNA ratio 1.8/1). .. Resulting double-stranded DNA was then digested with BpmI for 2 h at 37 °C, purified again with SPRI beads (beads/DNA ratio 1.5/1) and eluted in 13 μl ddH2 ).

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  • 99
    New England Biolabs second strand synthesis reaction buffer
    Second Strand Synthesis Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/second strand synthesis reaction buffer/product/New England Biolabs
    Average 99 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    second strand synthesis reaction buffer - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

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