lysis buffer  (New England Biolabs)


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    Name:
    Luciferase Cell Lysis Buffer
    Description:
    Luciferase Cell Lysis Buffer 25 0 ml
    Catalog Number:
    b3321s
    Price:
    58
    Size:
    25 0 ml
    Category:
    Cellular Biology
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    New England Biolabs lysis buffer
    Luciferase Cell Lysis Buffer
    Luciferase Cell Lysis Buffer 25 0 ml
    https://www.bioz.com/result/lysis buffer/product/New England Biolabs
    Average 95 stars, based on 510 article reviews
    Price from $9.99 to $1999.99
    lysis buffer - by Bioz Stars, 2020-08
    95/100 stars

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    Related Articles

    Lysis:

    Article Title: Codon usage bias controls mRNA and protein abundance in trypanosomatids
    Article Snippet: .. Lysates for gLUC assays were prepared by adding 20 µl of 1 × luciferase cell lysis buffer (New England Biolabs) to 2 × 106 pelleted cells and these were directly used to perform BioLux Gaussia luciferase assays (New England Biolabs) according to the manufacturers’ instructions and using a TopCount plate-reader with white-walled plates. .. Lysates for gLUC assays were prepared by adding 20 µl of 1 × luciferase cell lysis buffer (New England Biolabs) to 2 × 106 pelleted cells and these were directly used to perform BioLux Gaussia luciferase assays (New England Biolabs) according to the manufacturers’ instructions and using a TopCount plate-reader with white-walled plates.

    Article Title: Endoplasmic reticulum stress eIF2α–ATF4 pathway-mediated cyclooxygenase-2 induction regulates cadmium-induced autophagy in kidney
    Article Snippet: .. Fluc activity was similarly analyzed: 20 μ l luciferase cell lysis buffer (NEB, Ipswich, MA, USA) was added to cellular extract, and then 100 μ l of the Fluc assay reagent (Lulong, Xiamen, China) was added to the cell lysate and read immediately. .. SEAP assay SEAP was detected by using the Phospha-Light EXP assay kit (Life) according to the manufacturer's manuals.

    Article Title: Human Rhinovirus 16 Causes Golgi Apparatus Fragmentation without Blocking Protein Secretion
    Article Snippet: .. To determine the Gluc activity in the cells, the wells were washed with PBS and the cells lysed in 250 μl of luciferase cell lysis buffer (B3321; New England BioLabs). .. Gluc secreted in the media and remaining in the cells was assayed using the BioLux Gaussia luciferase flex assay kit (E3308S; New England BioLabs) according to the manufacturer's instructions.

    Article Title: Multicellular Tumor Spheroids as a Model for Assessing Delivery of Oligonucleotides in Three Dimensions
    Article Snippet: .. Cells were washed with phosphate-buffered saline before being digested with luciferase lysis buffer (New England Biolabs, Ipswich, MA) at a dilution of 1:4. .. After centrifugation, 50 µl of luciferin substrate (Promega, Madison, WI) was added to 10 µl of lysate, and luciferase activity was measured in a plate reader (FLUOstar Omega; BMG Labtech, Cary, NC) over a 5-second window.

    Article Title: Analysis by a highly sensitive split luciferase assay of the regions involved in APP dimerization and its impact on processing
    Article Snippet: .. The luciferase cell lysis buffer was from New England Biolabs (Ipswich, MA). .. The following primary antibodies were used: anti-amyloid β antibody, clone W0-2 (EMD Millipore, Billerica, MA), anti-amyloid precursor protein, C-terminal antibody (Sigma-Aldrich, St Louis, MO), anti-GLuc antibody (New England Biolabs, Ipswich, MA).

    Article Title: Multiple Antibody Lineages in One Donor Target the Glycan-V3 Supersite of the HIV-1 Envelope Glycoprotein and Display a Preference for Quaternary Binding
    Article Snippet: .. After 14 days of culture, 40 μl of the culture supernatant was removed for microneutralization screening, and B cells were frozen in lysis buffer containing 0.25 μl of an RNase inhibitor (New England BioLabs), 0.3 μl of 1 M Tris (pH 8), and 19.45 μl diethyl pyrocarbonate (DEPC)-treated H2 O. .. The high-throughput NVITAL automated microneutralization qualitative assay was developed from the well-characterized TZM-bl HIV serum assay utilizing pseudotyped virus expressing the envelope antigen and the luciferase reporter gene ( ).

    Article Title: Legionella remodels the plasma membrane–derived vacuole by utilizing exocyst components as tethers
    Article Snippet: .. To assay tethering, 100 µl lysis buffer (100 mM NaCl, 1 mM MgCl2 , 20 mM Hepes-KOH, pH 7.2, 1% Triton X-100, and protease inhibitors) was added to the cells to liberate Luciferase-KDEL from vesicles, and activity was measured using a Luciferase assay kit (New England Biolabs). .. Infection, immunoprecipitation, and immunofluorescence analysis Infection of HEK293-FcγRII cells with Legionella and generation of cell lysates for immunoprecipitation studies were conducted as described previously ( ).

    Luciferase:

    Article Title: Codon usage bias controls mRNA and protein abundance in trypanosomatids
    Article Snippet: .. Lysates for gLUC assays were prepared by adding 20 µl of 1 × luciferase cell lysis buffer (New England Biolabs) to 2 × 106 pelleted cells and these were directly used to perform BioLux Gaussia luciferase assays (New England Biolabs) according to the manufacturers’ instructions and using a TopCount plate-reader with white-walled plates. .. Lysates for gLUC assays were prepared by adding 20 µl of 1 × luciferase cell lysis buffer (New England Biolabs) to 2 × 106 pelleted cells and these were directly used to perform BioLux Gaussia luciferase assays (New England Biolabs) according to the manufacturers’ instructions and using a TopCount plate-reader with white-walled plates.

    Article Title: Endoplasmic reticulum stress eIF2α–ATF4 pathway-mediated cyclooxygenase-2 induction regulates cadmium-induced autophagy in kidney
    Article Snippet: .. Fluc activity was similarly analyzed: 20 μ l luciferase cell lysis buffer (NEB, Ipswich, MA, USA) was added to cellular extract, and then 100 μ l of the Fluc assay reagent (Lulong, Xiamen, China) was added to the cell lysate and read immediately. .. SEAP assay SEAP was detected by using the Phospha-Light EXP assay kit (Life) according to the manufacturer's manuals.

    Article Title: Human Rhinovirus 16 Causes Golgi Apparatus Fragmentation without Blocking Protein Secretion
    Article Snippet: .. To determine the Gluc activity in the cells, the wells were washed with PBS and the cells lysed in 250 μl of luciferase cell lysis buffer (B3321; New England BioLabs). .. Gluc secreted in the media and remaining in the cells was assayed using the BioLux Gaussia luciferase flex assay kit (E3308S; New England BioLabs) according to the manufacturer's instructions.

    Article Title: Multicellular Tumor Spheroids as a Model for Assessing Delivery of Oligonucleotides in Three Dimensions
    Article Snippet: .. Cells were washed with phosphate-buffered saline before being digested with luciferase lysis buffer (New England Biolabs, Ipswich, MA) at a dilution of 1:4. .. After centrifugation, 50 µl of luciferin substrate (Promega, Madison, WI) was added to 10 µl of lysate, and luciferase activity was measured in a plate reader (FLUOstar Omega; BMG Labtech, Cary, NC) over a 5-second window.

    Article Title: Analysis by a highly sensitive split luciferase assay of the regions involved in APP dimerization and its impact on processing
    Article Snippet: .. The luciferase cell lysis buffer was from New England Biolabs (Ipswich, MA). .. The following primary antibodies were used: anti-amyloid β antibody, clone W0-2 (EMD Millipore, Billerica, MA), anti-amyloid precursor protein, C-terminal antibody (Sigma-Aldrich, St Louis, MO), anti-GLuc antibody (New England Biolabs, Ipswich, MA).

    Article Title: Legionella remodels the plasma membrane–derived vacuole by utilizing exocyst components as tethers
    Article Snippet: .. To assay tethering, 100 µl lysis buffer (100 mM NaCl, 1 mM MgCl2 , 20 mM Hepes-KOH, pH 7.2, 1% Triton X-100, and protease inhibitors) was added to the cells to liberate Luciferase-KDEL from vesicles, and activity was measured using a Luciferase assay kit (New England Biolabs). .. Infection, immunoprecipitation, and immunofluorescence analysis Infection of HEK293-FcγRII cells with Legionella and generation of cell lysates for immunoprecipitation studies were conducted as described previously ( ).

    Activity Assay:

    Article Title: Endoplasmic reticulum stress eIF2α–ATF4 pathway-mediated cyclooxygenase-2 induction regulates cadmium-induced autophagy in kidney
    Article Snippet: .. Fluc activity was similarly analyzed: 20 μ l luciferase cell lysis buffer (NEB, Ipswich, MA, USA) was added to cellular extract, and then 100 μ l of the Fluc assay reagent (Lulong, Xiamen, China) was added to the cell lysate and read immediately. .. SEAP assay SEAP was detected by using the Phospha-Light EXP assay kit (Life) according to the manufacturer's manuals.

    Article Title: Human Rhinovirus 16 Causes Golgi Apparatus Fragmentation without Blocking Protein Secretion
    Article Snippet: .. To determine the Gluc activity in the cells, the wells were washed with PBS and the cells lysed in 250 μl of luciferase cell lysis buffer (B3321; New England BioLabs). .. Gluc secreted in the media and remaining in the cells was assayed using the BioLux Gaussia luciferase flex assay kit (E3308S; New England BioLabs) according to the manufacturer's instructions.

    Article Title: Legionella remodels the plasma membrane–derived vacuole by utilizing exocyst components as tethers
    Article Snippet: .. To assay tethering, 100 µl lysis buffer (100 mM NaCl, 1 mM MgCl2 , 20 mM Hepes-KOH, pH 7.2, 1% Triton X-100, and protease inhibitors) was added to the cells to liberate Luciferase-KDEL from vesicles, and activity was measured using a Luciferase assay kit (New England Biolabs). .. Infection, immunoprecipitation, and immunofluorescence analysis Infection of HEK293-FcγRII cells with Legionella and generation of cell lysates for immunoprecipitation studies were conducted as described previously ( ).

    Expressing:

    Article Title: DNA mismatch repair controls the host innate response and cell fate after influenza virus infection
    Article Snippet: .. To measure NanoLuc expression, cells were lysed with Luciferase Cell Lysis Buffer (NEB) and then evaluated using the Nano-Glo Luciferase Assay Reagent Kit (Promega). .. For phospho-H2AX staining, H441 cells were plated on collagen-coated cover slides and infected with WT PR8 (MOI=5) for indicated lengths of time before fixation with 4% PFA.

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  • 95
    New England Biolabs lysis buffer
    Lysis Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 342 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lysis buffer/product/New England Biolabs
    Average 95 stars, based on 342 article reviews
    Price from $9.99 to $1999.99
    lysis buffer - by Bioz Stars, 2020-08
    95/100 stars
      Buy from Supplier

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