nuclease free water  (New England Biolabs)


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  • 99
    Name:
    Nuclease free Water
    Description:
    Nuclease free Water 100 ml
    Catalog Number:
    b1500l
    Price:
    62
    Size:
    100 ml
    Category:
    Ribonuclease Protection Assays
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    Structured Review

    New England Biolabs nuclease free water
    Nuclease free Water
    Nuclease free Water 100 ml
    https://www.bioz.com/result/nuclease free water/product/New England Biolabs
    Average 99 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    nuclease free water - by Bioz Stars, 2020-10
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Early MinION™ nanopore single-molecule sequencing technology enables the characterization of hepatitis B virus genetic complexity in clinical samples
    Article Snippet: .. Briefly, the protocol for preparing a nanopore sequencing with SQK-MAP004 is as follows: 500 ng to 1μg of PCR products or cloned HBV DNA were diluted to 80μL using nuclease-free water and end-repaired using the NEBNext End Repair Module (New England Biolabs, Evry, France). .. The end-repaired products were then purified using 1.0 x volume of AMPure XP beads (Beckman-Coulter) and eluted in 25μL of nuclease-free.

    Purification:

    Article Title: Assessing the utility of the Oxford Nanopore MinION for snake venom gland cDNA sequencing
    Article Snippet: .. Second strand synthesis was performed with the NEBNext mRNA Second Strand Synthesis Module (New England BioLabs E6111; New England BioLabs, Ipswich, MA, USA), using 45 µl of nuclease-free water, 10 µl of NEBNext Second Strand Synthesis Reaction Buffer and 5 µl of NEBNext Second Strand Synthesis Enzyme Mix, with incubation at 16 °C for 1 h. Double-stranded cDNA (ds cDNA) was purified using a 1.8× volume of Agencourt AMPure XP beads (Beckman Coulter A63880), with a 5 min binding step (with gentle shaking), two washes in 200 µl 70% ethanol and elution in 51 µl nuclease-free water. .. End-repair and dA-tailing End-repair was performed using the NEBNext End Repair Module (New England BioLabs E6050; New England BioLabs, Ipswich, MA, USA) with 6 µl of 10× end-repair buffer and 3 µl of end-repair enzyme mix added to each of the 51 µl ds cDNA samples, followed by incubation at room temperature for 25 min and clean-up using a 1.8× volume of Agencourt beads (as above), with elution in 25 µl nuclease-free water.

    Article Title: HLA genotyping by next-generation sequencing of complementary DNA
    Article Snippet: .. Fragmentation of biotinylated PCR products For fragmentation of the biotinylated PCR products, a 20 μl solution containing 2.4 μl of 84.2 ng/μl purified biotinylated PCR products (200 ng), 2 μl of 10× Fragmentase Reaction Buffer v2 (NEB), 13.6 μl of Nuclease-Free water, and 2 μl dsDNA fragmentase (NEB) was incubated at 37 °C for 5 min, and 7.5 μl of 0.5 M EDTA was added to inactivate the enzyme. .. Fragmented PCR products were purified using AMPure XP (recovery volume: 20 μl).

    Incubation:

    Article Title: Assessing the utility of the Oxford Nanopore MinION for snake venom gland cDNA sequencing
    Article Snippet: .. Second strand synthesis was performed with the NEBNext mRNA Second Strand Synthesis Module (New England BioLabs E6111; New England BioLabs, Ipswich, MA, USA), using 45 µl of nuclease-free water, 10 µl of NEBNext Second Strand Synthesis Reaction Buffer and 5 µl of NEBNext Second Strand Synthesis Enzyme Mix, with incubation at 16 °C for 1 h. Double-stranded cDNA (ds cDNA) was purified using a 1.8× volume of Agencourt AMPure XP beads (Beckman Coulter A63880), with a 5 min binding step (with gentle shaking), two washes in 200 µl 70% ethanol and elution in 51 µl nuclease-free water. .. End-repair and dA-tailing End-repair was performed using the NEBNext End Repair Module (New England BioLabs E6050; New England BioLabs, Ipswich, MA, USA) with 6 µl of 10× end-repair buffer and 3 µl of end-repair enzyme mix added to each of the 51 µl ds cDNA samples, followed by incubation at room temperature for 25 min and clean-up using a 1.8× volume of Agencourt beads (as above), with elution in 25 µl nuclease-free water.

    Article Title: HLA genotyping by next-generation sequencing of complementary DNA
    Article Snippet: .. Fragmentation of biotinylated PCR products For fragmentation of the biotinylated PCR products, a 20 μl solution containing 2.4 μl of 84.2 ng/μl purified biotinylated PCR products (200 ng), 2 μl of 10× Fragmentase Reaction Buffer v2 (NEB), 13.6 μl of Nuclease-Free water, and 2 μl dsDNA fragmentase (NEB) was incubated at 37 °C for 5 min, and 7.5 μl of 0.5 M EDTA was added to inactivate the enzyme. .. Fragmented PCR products were purified using AMPure XP (recovery volume: 20 μl).

    other:

    Article Title: Long-adapter single-strand oligonucleotide probes for the massively multiplexed cloning of kilobase genome regions
    Article Snippet: Linear DNA Digestion Solution (volume of 48 μl) was composed of 24μl of nuclease free water, 6 μl of Exonuclease I (20 units/μl), 6 μl of Exonuclease III (100 units/μl), 6 μl of Lambda Exonuclease (5 units/μl) and 6 μl Exonuclease VII (10 units/μl) (all from NEB).

    Polymerase Chain Reaction:

    Article Title: Combination of microbiome analysis and serodiagnostics to assess the risk of pathogen transmission by ticks to humans and animals in central Germany
    Article Snippet: .. Each PCR reaction was performed in a total of 50 μl of reaction solution comrpising 14 μl of nuclease-free water, 10 ng of input DNA, 1 μl of barcode and 25 μl of LongAmp Taq 2X Mastermix (New England Biolabs, Frankfurt, Germany). .. The following PCR protocol was performed: 95 °C for 1 min; 25 cycles at 95 °C for 20 s, 55 °C for 30 s and 65 °C for 2 min, and a final extension step at 72 °C for 5 min. PCR products were purified using 30 μl of AMPure XP DNA beads (Beckman Coulter).

    Article Title: HLA genotyping by next-generation sequencing of complementary DNA
    Article Snippet: .. Fragmentation of biotinylated PCR products For fragmentation of the biotinylated PCR products, a 20 μl solution containing 2.4 μl of 84.2 ng/μl purified biotinylated PCR products (200 ng), 2 μl of 10× Fragmentase Reaction Buffer v2 (NEB), 13.6 μl of Nuclease-Free water, and 2 μl dsDNA fragmentase (NEB) was incubated at 37 °C for 5 min, and 7.5 μl of 0.5 M EDTA was added to inactivate the enzyme. .. Fragmented PCR products were purified using AMPure XP (recovery volume: 20 μl).

    Article Title: Early MinION™ nanopore single-molecule sequencing technology enables the characterization of hepatitis B virus genetic complexity in clinical samples
    Article Snippet: .. Briefly, the protocol for preparing a nanopore sequencing with SQK-MAP004 is as follows: 500 ng to 1μg of PCR products or cloned HBV DNA were diluted to 80μL using nuclease-free water and end-repaired using the NEBNext End Repair Module (New England Biolabs, Evry, France). .. The end-repaired products were then purified using 1.0 x volume of AMPure XP beads (Beckman-Coulter) and eluted in 25μL of nuclease-free.

    Article Title: RNA Framework: an all-in-one toolkit for the analysis of RNA structures and post-transcriptional modifications
    Article Snippet: .. Template RNA was degraded by adding 1 μl of 5 M NaOH and incubating at 95°C for 3 min. After reaction cleanup, cDNA was eluted in 20 μl nuclease-free water, and barcodes were introduced by 15 cycles of PCR in the presence of 25 pmol of each primer, and 25 μl NEBNext® High-Fidelity 2× PCR Master Mix. .. DMS was diluted 1:6 in 100% ethanol to a final concentration of 1.76 M. Diluted DMS was added to bacteria to a final concentration of ∼105 mM.

    Binding Assay:

    Article Title: Assessing the utility of the Oxford Nanopore MinION for snake venom gland cDNA sequencing
    Article Snippet: .. Second strand synthesis was performed with the NEBNext mRNA Second Strand Synthesis Module (New England BioLabs E6111; New England BioLabs, Ipswich, MA, USA), using 45 µl of nuclease-free water, 10 µl of NEBNext Second Strand Synthesis Reaction Buffer and 5 µl of NEBNext Second Strand Synthesis Enzyme Mix, with incubation at 16 °C for 1 h. Double-stranded cDNA (ds cDNA) was purified using a 1.8× volume of Agencourt AMPure XP beads (Beckman Coulter A63880), with a 5 min binding step (with gentle shaking), two washes in 200 µl 70% ethanol and elution in 51 µl nuclease-free water. .. End-repair and dA-tailing End-repair was performed using the NEBNext End Repair Module (New England BioLabs E6050; New England BioLabs, Ipswich, MA, USA) with 6 µl of 10× end-repair buffer and 3 µl of end-repair enzyme mix added to each of the 51 µl ds cDNA samples, followed by incubation at room temperature for 25 min and clean-up using a 1.8× volume of Agencourt beads (as above), with elution in 25 µl nuclease-free water.

    Nanopore Sequencing:

    Article Title: Early MinION™ nanopore single-molecule sequencing technology enables the characterization of hepatitis B virus genetic complexity in clinical samples
    Article Snippet: .. Briefly, the protocol for preparing a nanopore sequencing with SQK-MAP004 is as follows: 500 ng to 1μg of PCR products or cloned HBV DNA were diluted to 80μL using nuclease-free water and end-repaired using the NEBNext End Repair Module (New England Biolabs, Evry, France). .. The end-repaired products were then purified using 1.0 x volume of AMPure XP beads (Beckman-Coulter) and eluted in 25μL of nuclease-free.

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  • About
  • News
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  • Team
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  • Bioz Stars
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  • 99
    New England Biolabs nuclease free water
    Nuclease Free Water, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nuclease free water/product/New England Biolabs
    Average 99 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    nuclease free water - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

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