magnesium sulfate mgso4 solution  (New England Biolabs)


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    Name:
    Magnesium Sulfate MgSO4 Solution
    Description:
    Magnesium Sulfate MgSO4 Solution 6 0 ml
    Catalog Number:
    b1003s
    Price:
    23
    Size:
    6 0 ml
    Category:
    General Chemical Solutions
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    New England Biolabs magnesium sulfate mgso4 solution
    Magnesium Sulfate MgSO4 Solution
    Magnesium Sulfate MgSO4 Solution 6 0 ml
    https://www.bioz.com/result/magnesium sulfate mgso4 solution/product/New England Biolabs
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    magnesium sulfate mgso4 solution - by Bioz Stars, 2020-02
    94/100 stars

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    High Performance Liquid Chromatography:

    Article Title: Single-ribonucleotide repair-mediated ligation-dependent cycling signal amplification for sensitive and specific detection of DNA methyltransferase repair-mediated ligation-dependent cycling signal amplification for sensitive and specific detection of DNA methyltransferase †Electronic supplementary
    Article Snippet: DNA adenine methyltransferase (Dam MTase), 10× Dam MTase reaction buffer (500 mM trizma hydrochloride (Tris–HCl), 50 mM 2-mercaptoethanol (β-ME), 100 mM ethylenediaminetetraacetic acid (EDTA), pH 7.5), CpG methyltransferase (M.SssI), GpC methyltransferase (M.CviPI), Dpn I, 10× CutSmart buffer (500 mM potassium acetate (KAc), 200 mM tris-acetate, 100 mM magnesium acetate (Mg(Ac)2 ), 1 mg mL–1 bovine serum albumin (BSA), pH 7.9), S -adenosylmethionine (SAM), Taq DNA ligase, 10× Taq DNA ligase reaction buffer (200 mM Tris–HCl, 250 mM KAc, 100 mM Mg(Ac)2 , 100 mM DL-dithiothreitol (DTT), 10 mM nicotinamide adenine dinucleotide (NAD), 1% triton X-100, pH 7.6), Bst DNA polymerase (large fragment), 10× ThermoPol reaction buffer (200 mM Tris–HCl, 100 mM potassium chloride (KCl), 100 mM ammonium sulfate (NH4 )2 SO4 ), 20 mM magnesium sulfate (MgSO4 ), 1% triton X-100, pH 8.8), ribonuclease HII (RNase HII), deoxyadenosine triphosphate (dATP), deoxyuridine triphosphate (dUTP), deoxyguanosine triphosphate (dGTP) and deoxycytidine triphosphate (dCTP) were purchased from New England Biolabs (Ipswich, MA, USA). .. DNA adenine methyltransferase (Dam MTase), 10× Dam MTase reaction buffer (500 mM trizma hydrochloride (Tris–HCl), 50 mM 2-mercaptoethanol (β-ME), 100 mM ethylenediaminetetraacetic acid (EDTA), pH 7.5), CpG methyltransferase (M.SssI), GpC methyltransferase (M.CviPI), Dpn I, 10× CutSmart buffer (500 mM potassium acetate (KAc), 200 mM tris-acetate, 100 mM magnesium acetate (Mg(Ac)2 ), 1 mg mL–1 bovine serum albumin (BSA), pH 7.9), S -adenosylmethionine (SAM), Taq DNA ligase, 10× Taq DNA ligase reaction buffer (200 mM Tris–HCl, 250 mM KAc, 100 mM Mg(Ac)2 , 100 mM DL-dithiothreitol (DTT), 10 mM nicotinamide adenine dinucleotide (NAD), 1% triton X-100, pH 7.6), Bst DNA polymerase (large fragment), 10× ThermoPol reaction buffer (200 mM Tris–HCl, 100 mM potassium chloride (KCl), 100 mM ammonium sulfate (NH4 )2 SO4 ), 20 mM magnesium sulfate (MgSO4 ), 1% triton X-100, pH 8.8), ribonuclease HII (RNase HII), deoxyadenosine triphosphate (dATP), deoxyuridine triphosphate (dUTP), deoxyguanosine triphosphate (dGTP) and deoxycytidine triphosphate (dCTP) were purchased from New England Biolabs (Ipswich, MA, USA).

    Article Title: Single-ribonucleotide repair-mediated ligation-dependent cycling signal amplification for sensitive and specific detection of DNA methyltransferase repair-mediated ligation-dependent cycling signal amplification for sensitive and specific detection of DNA methyltransferase †Electronic supplementary
    Article Snippet: DNA adenine methyltransferase (Dam MTase), 10× Dam MTase reaction buffer (500 mM trizma hydrochloride (Tris–HCl), 50 mM 2-mercaptoethanol (β-ME), 100 mM ethylenediaminetetraacetic acid (EDTA), pH 7.5), CpG methyltransferase (M.SssI), GpC methyltransferase (M.CviPI), Dpn I, 10× CutSmart buffer (500 mM potassium acetate (KAc), 200 mM tris-acetate, 100 mM magnesium acetate (Mg(Ac)2 ), 1 mg mL–1 bovine serum albumin (BSA), pH 7.9), S -adenosylmethionine (SAM), Taq DNA ligase, 10× Taq DNA ligase reaction buffer (200 mM Tris–HCl, 250 mM KAc, 100 mM Mg(Ac)2 , 100 mM DL-dithiothreitol (DTT), 10 mM nicotinamide adenine dinucleotide (NAD), 1% triton X-100, pH 7.6), Bst DNA polymerase (large fragment), 10× ThermoPol reaction buffer (200 mM Tris–HCl, 100 mM potassium chloride (KCl), 100 mM ammonium sulfate (NH4 )2 SO4 ), 20 mM magnesium sulfate (MgSO4 ), 1% triton X-100, pH 8.8), ribonuclease HII (RNase HII), deoxyadenosine triphosphate (dATP), deoxyuridine triphosphate (dUTP), deoxyguanosine triphosphate (dGTP) and deoxycytidine triphosphate (dCTP) were purchased from New England Biolabs (Ipswich, MA, USA). .. DNA adenine methyltransferase (Dam MTase), 10× Dam MTase reaction buffer (500 mM trizma hydrochloride (Tris–HCl), 50 mM 2-mercaptoethanol (β-ME), 100 mM ethylenediaminetetraacetic acid (EDTA), pH 7.5), CpG methyltransferase (M.SssI), GpC methyltransferase (M.CviPI), Dpn I, 10× CutSmart buffer (500 mM potassium acetate (KAc), 200 mM tris-acetate, 100 mM magnesium acetate (Mg(Ac)2 ), 1 mg mL–1 bovine serum albumin (BSA), pH 7.9), S -adenosylmethionine (SAM), Taq DNA ligase, 10× Taq DNA ligase reaction buffer (200 mM Tris–HCl, 250 mM KAc, 100 mM Mg(Ac)2 , 100 mM DL-dithiothreitol (DTT), 10 mM nicotinamide adenine dinucleotide (NAD), 1% triton X-100, pH 7.6), Bst DNA polymerase (large fragment), 10× ThermoPol reaction buffer (200 mM Tris–HCl, 100 mM potassium chloride (KCl), 100 mM ammonium sulfate (NH4 )2 SO4 ), 20 mM magnesium sulfate (MgSO4 ), 1% triton X-100, pH 8.8), ribonuclease HII (RNase HII), deoxyadenosine triphosphate (dATP), deoxyuridine triphosphate (dUTP), deoxyguanosine triphosphate (dGTP) and deoxycytidine triphosphate (dCTP) were purchased from New England Biolabs (Ipswich, MA, USA).

    DNA Extraction:

    Article Title: Real-Time, Digital LAMP with Commercial Microfluidic Chips Reveals the Interplay of Efficiency, Speed, and Background Amplification as a Function of Reaction Temperature and Time
    Article Snippet: LAMP experiments using Bst 2.0 ( a,c,e,g) contained the following final concentrations, optimized as shown in Supporting Information, Figure S3 : 1× Isothermal Amplification Buffer (New England BioLabs; ref B0537S, containing 20 mM Tris-HCl 10 mM (NH4 )2 SO4 , 50 mM KCl, 2 mM MgSO4 , 0.1% Tween 20 pH 8.8 at 25 °C), additional 6 mM MgSO4 (New England BioLabs; ref B1003S), 1.4 mM Deoxynucleotide Solution Mix (New England BioLabs; ref N0447S). .. Template E. coli DNA was extracted from exponential-phase cultures grown in BBL Brain–Heart Infusion media (BD, Franklin Lakes, NJ, USA; ref 221813) using QuickExtract DNA Extraction Solution (Lucigen, Middleton, WI, USA; ref QE09050) as described previously.

    Article Title: Real-Time, Digital LAMP with Commercial Microfluidic Chips Reveals the Interplay of Efficiency, Speed, and Background Amplification as a Function of Reaction Temperature and Time
    Article Snippet: Figure LAMP experiments using Bst 2.0 ( : 1× Isothermal Amplification Buffer (New England BioLabs; ref B0537S, containing 20 mM Tris-HCl 10 mM (NH4 )2 SO4 , 50 mM KCl, 2 mM MgSO4 , 0.1% Tween 20 pH 8.8 at 25 °C), additional 6 mM MgSO4 (New England BioLabs; ref B1003S), 1.4 mM Deoxynucleotide Solution Mix (New England BioLabs; ref N0447S). .. Template E. coli DNA was extracted from exponential-phase cultures grown in BBL Brain–Heart Infusion media (BD, Franklin Lakes, NJ, USA; ref 221813) using QuickExtract DNA Extraction Solution (Lucigen, Middleton, WI, USA; ref QE09050) as described previously.

    Amplification:

    Article Title: Surveillance for Western Equine Encephalitis, St. Louis Encephalitis, and West Nile Viruses Using Reverse Transcription Loop-Mediated Isothermal Amplification
    Article Snippet: .. The reaction mixture had a final composition (after adding water or template) of 1X Isothermal Amplification Buffer (New England Biolabs, NEB #B0537S) supplemented with an additional 6 mM MgSO4 (NEB #B1003S, final 8 mM MgSO4 ), 0.8 M Betaine (Sigma #B-0300), 1.4 mM each dNTP (NEB #N0447L), 0.32 units/μL Bst 2.0 WarmStart DNA polymerase (NEB #M0538M), 0.2 units/μL AMV reverse transcriptase (NEB #M0277T, or Life Science Advanced Technologies #AMVRTT-5), and 2 μM (or in some instances 4 μM) SYTO 82 detection dye (Life Technologies #S11363) [ ]. ..

    Article Title: Real-Time, Digital LAMP with Commercial Microfluidic Chips Reveals the Interplay of Efficiency, Speed, and Background Amplification as a Function of Reaction Temperature and Time
    Article Snippet: .. LAMP experiments using Bst 2.0 ( a,c,e,g) contained the following final concentrations, optimized as shown in Supporting Information, Figure S3 : 1× Isothermal Amplification Buffer (New England BioLabs; ref B0537S, containing 20 mM Tris-HCl 10 mM (NH4 )2 SO4 , 50 mM KCl, 2 mM MgSO4 , 0.1% Tween 20 pH 8.8 at 25 °C), additional 6 mM MgSO4 (New England BioLabs; ref B1003S), 1.4 mM Deoxynucleotide Solution Mix (New England BioLabs; ref N0447S). .. Primers: 1.6 μM FIP/BIP, 0.2 μM FOP/BOP, and 0.4 μM LoopF/LoopB, 1 mg/mL BSA (New England BioLabs; ref B90005), 320 U/mL Bst 2.0 (New England BioLabs; ref M0537S), Ambion RNase cocktail (ThermoFisher, ref AM2286, 5 U/mL RNase A, 400 U/mL TNase T1), 2 μM SYTO 9 (ThermoFisher, ref S34854), and approximately 660 copies/μL template in Ambion nuclease-free water (ThermoFisher; ref AM9932).

    Article Title: Real-Time, Digital LAMP with Commercial Microfluidic Chips Reveals the Interplay of Efficiency, Speed, and Background Amplification as a Function of Reaction Temperature and Time
    Article Snippet: .. Figure LAMP experiments using Bst 2.0 ( : 1× Isothermal Amplification Buffer (New England BioLabs; ref B0537S, containing 20 mM Tris-HCl 10 mM (NH4 )2 SO4 , 50 mM KCl, 2 mM MgSO4 , 0.1% Tween 20 pH 8.8 at 25 °C), additional 6 mM MgSO4 (New England BioLabs; ref B1003S), 1.4 mM Deoxynucleotide Solution Mix (New England BioLabs; ref N0447S). .. Primers: 1.6 μM FIP/BIP, 0.2 μM FOP/BOP, and 0.4 μM LoopF/LoopB, 1 mg/mL BSA (New England BioLabs; ref B90005), 320 U/mL Bst 2.0 (New England BioLabs; ref M0537S), Ambion RNase cocktail (ThermoFisher, ref AM2286, 5 U/mL RNase A, 400 U/mL TNase T1), 2 μM SYTO 9 (ThermoFisher, ref S34854), and approximately 660 copies/μL template in Ambion nuclease-free water (ThermoFisher; ref AM9932).

    Article Title: Thirty-minute screening of antibiotic resistance genes in bacterial isolates with minimal sample preparation in static self-dispensing 64 and 384 assay cards
    Article Snippet: Paragraph title: LAMP amplification ... Briefly, reaction mixtures contained 1× Bst DNA polymerase buffer (M0275L, New England Biolabs, Ipswich, MA, USA), 1.4 mM of each dNTP (10297018, Life Technologies, Grand Island, NY, USA), 800 mM betaine (B0300, Sigma Aldrich, St. Louis, MO, USA), 6 mM MgSO4 (B1003S, New England Biolabs, Ipswich, MA, USA), 1× primer mix (1.6 μM FIP and BIP, 800 nM LF and LB and 200 nM F3 and B3 primers), 1 mg/mL bovine serum albumin (B9000S, New England Biolabs, Ipswich, MA, USA), 20 μM SYTO-81 (S11362, Molecular Probes, Eugene, OR, USA [product not available anymore, can be replaced with SYTO-82, S11363, Life Technologies, Grand Island, NY, USA]), 0.2 % Pluronic F-68 (24040032, Life Technologies, Grand Island, NY, USA), and 0.64 U/μL Bst DNA polymerase (large fragment) (M0275L, New England Biolabs, Ipswich, MA, USA).

    Fluorescence:

    Article Title: Surveillance for Western Equine Encephalitis, St. Louis Encephalitis, and West Nile Viruses Using Reverse Transcription Loop-Mediated Isothermal Amplification
    Article Snippet: The reaction mixture had a final composition (after adding water or template) of 1X Isothermal Amplification Buffer (New England Biolabs, NEB #B0537S) supplemented with an additional 6 mM MgSO4 (NEB #B1003S, final 8 mM MgSO4 ), 0.8 M Betaine (Sigma #B-0300), 1.4 mM each dNTP (NEB #N0447L), 0.32 units/μL Bst 2.0 WarmStart DNA polymerase (NEB #M0538M), 0.2 units/μL AMV reverse transcriptase (NEB #M0277T, or Life Science Advanced Technologies #AMVRTT-5), and 2 μM (or in some instances 4 μM) SYTO 82 detection dye (Life Technologies #S11363) [ ]. .. RT-LAMP with real-time fluorescence monitoring was carried out in a BioRad CFX96, using detection channel 2 (HEX) for monitoring SYTO 82 dye.

    Synthesized:

    Article Title: Single-ribonucleotide repair-mediated ligation-dependent cycling signal amplification for sensitive and specific detection of DNA methyltransferase repair-mediated ligation-dependent cycling signal amplification for sensitive and specific detection of DNA methyltransferase †Electronic supplementary
    Article Snippet: DNA adenine methyltransferase (Dam MTase), 10× Dam MTase reaction buffer (500 mM trizma hydrochloride (Tris–HCl), 50 mM 2-mercaptoethanol (β-ME), 100 mM ethylenediaminetetraacetic acid (EDTA), pH 7.5), CpG methyltransferase (M.SssI), GpC methyltransferase (M.CviPI), Dpn I, 10× CutSmart buffer (500 mM potassium acetate (KAc), 200 mM tris-acetate, 100 mM magnesium acetate (Mg(Ac)2 ), 1 mg mL–1 bovine serum albumin (BSA), pH 7.9), S -adenosylmethionine (SAM), Taq DNA ligase, 10× Taq DNA ligase reaction buffer (200 mM Tris–HCl, 250 mM KAc, 100 mM Mg(Ac)2 , 100 mM DL-dithiothreitol (DTT), 10 mM nicotinamide adenine dinucleotide (NAD), 1% triton X-100, pH 7.6), Bst DNA polymerase (large fragment), 10× ThermoPol reaction buffer (200 mM Tris–HCl, 100 mM potassium chloride (KCl), 100 mM ammonium sulfate (NH4 )2 SO4 ), 20 mM magnesium sulfate (MgSO4 ), 1% triton X-100, pH 8.8), ribonuclease HII (RNase HII), deoxyadenosine triphosphate (dATP), deoxyuridine triphosphate (dUTP), deoxyguanosine triphosphate (dGTP) and deoxycytidine triphosphate (dCTP) were purchased from New England Biolabs (Ipswich, MA, USA). .. DNA adenine methyltransferase (Dam MTase), 10× Dam MTase reaction buffer (500 mM trizma hydrochloride (Tris–HCl), 50 mM 2-mercaptoethanol (β-ME), 100 mM ethylenediaminetetraacetic acid (EDTA), pH 7.5), CpG methyltransferase (M.SssI), GpC methyltransferase (M.CviPI), Dpn I, 10× CutSmart buffer (500 mM potassium acetate (KAc), 200 mM tris-acetate, 100 mM magnesium acetate (Mg(Ac)2 ), 1 mg mL–1 bovine serum albumin (BSA), pH 7.9), S -adenosylmethionine (SAM), Taq DNA ligase, 10× Taq DNA ligase reaction buffer (200 mM Tris–HCl, 250 mM KAc, 100 mM Mg(Ac)2 , 100 mM DL-dithiothreitol (DTT), 10 mM nicotinamide adenine dinucleotide (NAD), 1% triton X-100, pH 7.6), Bst DNA polymerase (large fragment), 10× ThermoPol reaction buffer (200 mM Tris–HCl, 100 mM potassium chloride (KCl), 100 mM ammonium sulfate (NH4 )2 SO4 ), 20 mM magnesium sulfate (MgSO4 ), 1% triton X-100, pH 8.8), ribonuclease HII (RNase HII), deoxyadenosine triphosphate (dATP), deoxyuridine triphosphate (dUTP), deoxyguanosine triphosphate (dGTP) and deoxycytidine triphosphate (dCTP) were purchased from New England Biolabs (Ipswich, MA, USA).

    Article Title: Single-ribonucleotide repair-mediated ligation-dependent cycling signal amplification for sensitive and specific detection of DNA methyltransferase repair-mediated ligation-dependent cycling signal amplification for sensitive and specific detection of DNA methyltransferase †Electronic supplementary
    Article Snippet: DNA adenine methyltransferase (Dam MTase), 10× Dam MTase reaction buffer (500 mM trizma hydrochloride (Tris–HCl), 50 mM 2-mercaptoethanol (β-ME), 100 mM ethylenediaminetetraacetic acid (EDTA), pH 7.5), CpG methyltransferase (M.SssI), GpC methyltransferase (M.CviPI), Dpn I, 10× CutSmart buffer (500 mM potassium acetate (KAc), 200 mM tris-acetate, 100 mM magnesium acetate (Mg(Ac)2 ), 1 mg mL–1 bovine serum albumin (BSA), pH 7.9), S -adenosylmethionine (SAM), Taq DNA ligase, 10× Taq DNA ligase reaction buffer (200 mM Tris–HCl, 250 mM KAc, 100 mM Mg(Ac)2 , 100 mM DL-dithiothreitol (DTT), 10 mM nicotinamide adenine dinucleotide (NAD), 1% triton X-100, pH 7.6), Bst DNA polymerase (large fragment), 10× ThermoPol reaction buffer (200 mM Tris–HCl, 100 mM potassium chloride (KCl), 100 mM ammonium sulfate (NH4 )2 SO4 ), 20 mM magnesium sulfate (MgSO4 ), 1% triton X-100, pH 8.8), ribonuclease HII (RNase HII), deoxyadenosine triphosphate (dATP), deoxyuridine triphosphate (dUTP), deoxyguanosine triphosphate (dGTP) and deoxycytidine triphosphate (dCTP) were purchased from New England Biolabs (Ipswich, MA, USA). .. DNA adenine methyltransferase (Dam MTase), 10× Dam MTase reaction buffer (500 mM trizma hydrochloride (Tris–HCl), 50 mM 2-mercaptoethanol (β-ME), 100 mM ethylenediaminetetraacetic acid (EDTA), pH 7.5), CpG methyltransferase (M.SssI), GpC methyltransferase (M.CviPI), Dpn I, 10× CutSmart buffer (500 mM potassium acetate (KAc), 200 mM tris-acetate, 100 mM magnesium acetate (Mg(Ac)2 ), 1 mg mL–1 bovine serum albumin (BSA), pH 7.9), S -adenosylmethionine (SAM), Taq DNA ligase, 10× Taq DNA ligase reaction buffer (200 mM Tris–HCl, 250 mM KAc, 100 mM Mg(Ac)2 , 100 mM DL-dithiothreitol (DTT), 10 mM nicotinamide adenine dinucleotide (NAD), 1% triton X-100, pH 7.6), Bst DNA polymerase (large fragment), 10× ThermoPol reaction buffer (200 mM Tris–HCl, 100 mM potassium chloride (KCl), 100 mM ammonium sulfate (NH4 )2 SO4 ), 20 mM magnesium sulfate (MgSO4 ), 1% triton X-100, pH 8.8), ribonuclease HII (RNase HII), deoxyadenosine triphosphate (dATP), deoxyuridine triphosphate (dUTP), deoxyguanosine triphosphate (dGTP) and deoxycytidine triphosphate (dCTP) were purchased from New England Biolabs (Ipswich, MA, USA).

    Gel Permeation Chromatography:

    Article Title: Single-ribonucleotide repair-mediated ligation-dependent cycling signal amplification for sensitive and specific detection of DNA methyltransferase repair-mediated ligation-dependent cycling signal amplification for sensitive and specific detection of DNA methyltransferase †Electronic supplementary
    Article Snippet: .. DNA adenine methyltransferase (Dam MTase), 10× Dam MTase reaction buffer (500 mM trizma hydrochloride (Tris–HCl), 50 mM 2-mercaptoethanol (β-ME), 100 mM ethylenediaminetetraacetic acid (EDTA), pH 7.5), CpG methyltransferase (M.SssI), GpC methyltransferase (M.CviPI), Dpn I, 10× CutSmart buffer (500 mM potassium acetate (KAc), 200 mM tris-acetate, 100 mM magnesium acetate (Mg(Ac)2 ), 1 mg mL–1 bovine serum albumin (BSA), pH 7.9), S -adenosylmethionine (SAM), Taq DNA ligase, 10× Taq DNA ligase reaction buffer (200 mM Tris–HCl, 250 mM KAc, 100 mM Mg(Ac)2 , 100 mM DL-dithiothreitol (DTT), 10 mM nicotinamide adenine dinucleotide (NAD), 1% triton X-100, pH 7.6), Bst DNA polymerase (large fragment), 10× ThermoPol reaction buffer (200 mM Tris–HCl, 100 mM potassium chloride (KCl), 100 mM ammonium sulfate (NH4 )2 SO4 ), 20 mM magnesium sulfate (MgSO4 ), 1% triton X-100, pH 8.8), ribonuclease HII (RNase HII), deoxyadenosine triphosphate (dATP), deoxyuridine triphosphate (dUTP), deoxyguanosine triphosphate (dGTP) and deoxycytidine triphosphate (dCTP) were purchased from New England Biolabs (Ipswich, MA, USA). .. SYBR Gold was purchased from Life Technologies (Carlsbad, CA, USA).

    Article Title: Single-ribonucleotide repair-mediated ligation-dependent cycling signal amplification for sensitive and specific detection of DNA methyltransferase repair-mediated ligation-dependent cycling signal amplification for sensitive and specific detection of DNA methyltransferase †Electronic supplementary
    Article Snippet: .. DNA adenine methyltransferase (Dam MTase), 10× Dam MTase reaction buffer (500 mM trizma hydrochloride (Tris–HCl), 50 mM 2-mercaptoethanol (β-ME), 100 mM ethylenediaminetetraacetic acid (EDTA), pH 7.5), CpG methyltransferase (M.SssI), GpC methyltransferase (M.CviPI), Dpn I, 10× CutSmart buffer (500 mM potassium acetate (KAc), 200 mM tris-acetate, 100 mM magnesium acetate (Mg(Ac)2 ), 1 mg mL–1 bovine serum albumin (BSA), pH 7.9), S -adenosylmethionine (SAM), Taq DNA ligase, 10× Taq DNA ligase reaction buffer (200 mM Tris–HCl, 250 mM KAc, 100 mM Mg(Ac)2 , 100 mM DL-dithiothreitol (DTT), 10 mM nicotinamide adenine dinucleotide (NAD), 1% triton X-100, pH 7.6), Bst DNA polymerase (large fragment), 10× ThermoPol reaction buffer (200 mM Tris–HCl, 100 mM potassium chloride (KCl), 100 mM ammonium sulfate (NH4 )2 SO4 ), 20 mM magnesium sulfate (MgSO4 ), 1% triton X-100, pH 8.8), ribonuclease HII (RNase HII), deoxyadenosine triphosphate (dATP), deoxyuridine triphosphate (dUTP), deoxyguanosine triphosphate (dGTP) and deoxycytidine triphosphate (dCTP) were purchased from New England Biolabs (Ipswich, MA, USA). .. SYBR Gold was purchased from Life Technologies (Carlsbad, CA, USA).

    AST Assay:

    Article Title: Real-Time, Digital LAMP with Commercial Microfluidic Chips Reveals the Interplay of Efficiency, Speed, and Background Amplification as a Function of Reaction Temperature and Time
    Article Snippet: LAMP Reagents Our amplification target was the Escherichia coli 23S ribosomal gene, which we used previously as a target to perform rapid AST on clinical samples. .. LAMP experiments using Bst 2.0 ( a,c,e,g) contained the following final concentrations, optimized as shown in Supporting Information, Figure S3 : 1× Isothermal Amplification Buffer (New England BioLabs; ref B0537S, containing 20 mM Tris-HCl 10 mM (NH4 )2 SO4 , 50 mM KCl, 2 mM MgSO4 , 0.1% Tween 20 pH 8.8 at 25 °C), additional 6 mM MgSO4 (New England BioLabs; ref B1003S), 1.4 mM Deoxynucleotide Solution Mix (New England BioLabs; ref N0447S).

    Article Title: Real-Time, Digital LAMP with Commercial Microfluidic Chips Reveals the Interplay of Efficiency, Speed, and Background Amplification as a Function of Reaction Temperature and Time
    Article Snippet: Our amplification target was the Escherichia coli 23S ribosomal gene, which we used previously as a target to perform rapid AST on clinical samples. .. Figure LAMP experiments using Bst 2.0 ( : 1× Isothermal Amplification Buffer (New England BioLabs; ref B0537S, containing 20 mM Tris-HCl 10 mM (NH4 )2 SO4 , 50 mM KCl, 2 mM MgSO4 , 0.1% Tween 20 pH 8.8 at 25 °C), additional 6 mM MgSO4 (New England BioLabs; ref B1003S), 1.4 mM Deoxynucleotide Solution Mix (New England BioLabs; ref N0447S).

    Purification:

    Article Title: Single-ribonucleotide repair-mediated ligation-dependent cycling signal amplification for sensitive and specific detection of DNA methyltransferase repair-mediated ligation-dependent cycling signal amplification for sensitive and specific detection of DNA methyltransferase †Electronic supplementary
    Article Snippet: DNA adenine methyltransferase (Dam MTase), 10× Dam MTase reaction buffer (500 mM trizma hydrochloride (Tris–HCl), 50 mM 2-mercaptoethanol (β-ME), 100 mM ethylenediaminetetraacetic acid (EDTA), pH 7.5), CpG methyltransferase (M.SssI), GpC methyltransferase (M.CviPI), Dpn I, 10× CutSmart buffer (500 mM potassium acetate (KAc), 200 mM tris-acetate, 100 mM magnesium acetate (Mg(Ac)2 ), 1 mg mL–1 bovine serum albumin (BSA), pH 7.9), S -adenosylmethionine (SAM), Taq DNA ligase, 10× Taq DNA ligase reaction buffer (200 mM Tris–HCl, 250 mM KAc, 100 mM Mg(Ac)2 , 100 mM DL-dithiothreitol (DTT), 10 mM nicotinamide adenine dinucleotide (NAD), 1% triton X-100, pH 7.6), Bst DNA polymerase (large fragment), 10× ThermoPol reaction buffer (200 mM Tris–HCl, 100 mM potassium chloride (KCl), 100 mM ammonium sulfate (NH4 )2 SO4 ), 20 mM magnesium sulfate (MgSO4 ), 1% triton X-100, pH 8.8), ribonuclease HII (RNase HII), deoxyadenosine triphosphate (dATP), deoxyuridine triphosphate (dUTP), deoxyguanosine triphosphate (dGTP) and deoxycytidine triphosphate (dCTP) were purchased from New England Biolabs (Ipswich, MA, USA). .. DNA adenine methyltransferase (Dam MTase), 10× Dam MTase reaction buffer (500 mM trizma hydrochloride (Tris–HCl), 50 mM 2-mercaptoethanol (β-ME), 100 mM ethylenediaminetetraacetic acid (EDTA), pH 7.5), CpG methyltransferase (M.SssI), GpC methyltransferase (M.CviPI), Dpn I, 10× CutSmart buffer (500 mM potassium acetate (KAc), 200 mM tris-acetate, 100 mM magnesium acetate (Mg(Ac)2 ), 1 mg mL–1 bovine serum albumin (BSA), pH 7.9), S -adenosylmethionine (SAM), Taq DNA ligase, 10× Taq DNA ligase reaction buffer (200 mM Tris–HCl, 250 mM KAc, 100 mM Mg(Ac)2 , 100 mM DL-dithiothreitol (DTT), 10 mM nicotinamide adenine dinucleotide (NAD), 1% triton X-100, pH 7.6), Bst DNA polymerase (large fragment), 10× ThermoPol reaction buffer (200 mM Tris–HCl, 100 mM potassium chloride (KCl), 100 mM ammonium sulfate (NH4 )2 SO4 ), 20 mM magnesium sulfate (MgSO4 ), 1% triton X-100, pH 8.8), ribonuclease HII (RNase HII), deoxyadenosine triphosphate (dATP), deoxyuridine triphosphate (dUTP), deoxyguanosine triphosphate (dGTP) and deoxycytidine triphosphate (dCTP) were purchased from New England Biolabs (Ipswich, MA, USA).

    Article Title: Single-ribonucleotide repair-mediated ligation-dependent cycling signal amplification for sensitive and specific detection of DNA methyltransferase repair-mediated ligation-dependent cycling signal amplification for sensitive and specific detection of DNA methyltransferase †Electronic supplementary
    Article Snippet: DNA adenine methyltransferase (Dam MTase), 10× Dam MTase reaction buffer (500 mM trizma hydrochloride (Tris–HCl), 50 mM 2-mercaptoethanol (β-ME), 100 mM ethylenediaminetetraacetic acid (EDTA), pH 7.5), CpG methyltransferase (M.SssI), GpC methyltransferase (M.CviPI), Dpn I, 10× CutSmart buffer (500 mM potassium acetate (KAc), 200 mM tris-acetate, 100 mM magnesium acetate (Mg(Ac)2 ), 1 mg mL–1 bovine serum albumin (BSA), pH 7.9), S -adenosylmethionine (SAM), Taq DNA ligase, 10× Taq DNA ligase reaction buffer (200 mM Tris–HCl, 250 mM KAc, 100 mM Mg(Ac)2 , 100 mM DL-dithiothreitol (DTT), 10 mM nicotinamide adenine dinucleotide (NAD), 1% triton X-100, pH 7.6), Bst DNA polymerase (large fragment), 10× ThermoPol reaction buffer (200 mM Tris–HCl, 100 mM potassium chloride (KCl), 100 mM ammonium sulfate (NH4 )2 SO4 ), 20 mM magnesium sulfate (MgSO4 ), 1% triton X-100, pH 8.8), ribonuclease HII (RNase HII), deoxyadenosine triphosphate (dATP), deoxyuridine triphosphate (dUTP), deoxyguanosine triphosphate (dGTP) and deoxycytidine triphosphate (dCTP) were purchased from New England Biolabs (Ipswich, MA, USA). .. DNA adenine methyltransferase (Dam MTase), 10× Dam MTase reaction buffer (500 mM trizma hydrochloride (Tris–HCl), 50 mM 2-mercaptoethanol (β-ME), 100 mM ethylenediaminetetraacetic acid (EDTA), pH 7.5), CpG methyltransferase (M.SssI), GpC methyltransferase (M.CviPI), Dpn I, 10× CutSmart buffer (500 mM potassium acetate (KAc), 200 mM tris-acetate, 100 mM magnesium acetate (Mg(Ac)2 ), 1 mg mL–1 bovine serum albumin (BSA), pH 7.9), S -adenosylmethionine (SAM), Taq DNA ligase, 10× Taq DNA ligase reaction buffer (200 mM Tris–HCl, 250 mM KAc, 100 mM Mg(Ac)2 , 100 mM DL-dithiothreitol (DTT), 10 mM nicotinamide adenine dinucleotide (NAD), 1% triton X-100, pH 7.6), Bst DNA polymerase (large fragment), 10× ThermoPol reaction buffer (200 mM Tris–HCl, 100 mM potassium chloride (KCl), 100 mM ammonium sulfate (NH4 )2 SO4 ), 20 mM magnesium sulfate (MgSO4 ), 1% triton X-100, pH 8.8), ribonuclease HII (RNase HII), deoxyadenosine triphosphate (dATP), deoxyuridine triphosphate (dUTP), deoxyguanosine triphosphate (dGTP) and deoxycytidine triphosphate (dCTP) were purchased from New England Biolabs (Ipswich, MA, USA).

    Article Title: Generalized stochastic profiling of transcriptional regulatory heterogeneities in tissues, tumors, and cultured cells
    Article Snippet: Terminal transferase (Roche #03333574001, 400 U μl−1 ) 10× ThermoPol buffer (New England Biolabs #B9005S) 100 mM MgSO4 (New England Biolabs #B1003S) BSA (Roche #10711454001) Roche Taq polymerase (Roche #04728858001, 5 U μl−1 ) AL1 primer (200 nmol synthesis from IDT or MWG): ATTGGATCCAGGCCGCTCTGGACAAAATATGAATTCTTTTTTTTTTTTTTTTTTTTT TTT ▲ CRITICAL Long desalted primers will have varying purities depending on the manufacturer. .. 100× SYBR Green I (Invitrogen #S7563 diluted 100-fold in DMSO to 100×) 50 mM aminoallyl-dUTP (Ambion #1103015) High-Fidelity polymerase (Roche #11732650001, 3.5 U μl−1 ) 10× High-Fidelity PCR buffer without Mg2+ (included with Roche #11759175001) PureLink PCR Purification Kit (Invitrogen #46-6056) NaOAc (Calbiochem #567418) 20 mg/ml glycogen (Invitrogen #10814-010) NaHCO3 (Acros #21712500) Alexa Fluor 555 reactive dye decapack (Invitrogen # ) GEX hybridization buffer (included with Illumina #BD-103-0204 or equivalent)

    SYBR Green Assay:

    Article Title: Generalized stochastic profiling of transcriptional regulatory heterogeneities in tissues, tumors, and cultured cells
    Article Snippet: Terminal transferase (Roche #03333574001, 400 U μl−1 ) 10× ThermoPol buffer (New England Biolabs #B9005S) 100 mM MgSO4 (New England Biolabs #B1003S) BSA (Roche #10711454001) Roche Taq polymerase (Roche #04728858001, 5 U μl−1 ) AL1 primer (200 nmol synthesis from IDT or MWG): ATTGGATCCAGGCCGCTCTGGACAAAATATGAATTCTTTTTTTTTTTTTTTTTTTTT TTT ▲ CRITICAL Long desalted primers will have varying purities depending on the manufacturer. .. 100× SYBR Green I (Invitrogen #S7563 diluted 100-fold in DMSO to 100×) 50 mM aminoallyl-dUTP (Ambion #1103015) High-Fidelity polymerase (Roche #11732650001, 3.5 U μl−1 ) 10× High-Fidelity PCR buffer without Mg2+ (included with Roche #11759175001) PureLink PCR Purification Kit (Invitrogen #46-6056) NaOAc (Calbiochem #567418) 20 mg/ml glycogen (Invitrogen #10814-010) NaHCO3 (Acros #21712500) Alexa Fluor 555 reactive dye decapack (Invitrogen # ) GEX hybridization buffer (included with Illumina #BD-103-0204 or equivalent)

    Incubation:

    Article Title: Surveillance for Western Equine Encephalitis, St. Louis Encephalitis, and West Nile Viruses Using Reverse Transcription Loop-Mediated Isothermal Amplification
    Article Snippet: The reaction mixture had a final composition (after adding water or template) of 1X Isothermal Amplification Buffer (New England Biolabs, NEB #B0537S) supplemented with an additional 6 mM MgSO4 (NEB #B1003S, final 8 mM MgSO4 ), 0.8 M Betaine (Sigma #B-0300), 1.4 mM each dNTP (NEB #N0447L), 0.32 units/μL Bst 2.0 WarmStart DNA polymerase (NEB #M0538M), 0.2 units/μL AMV reverse transcriptase (NEB #M0277T, or Life Science Advanced Technologies #AMVRTT-5), and 2 μM (or in some instances 4 μM) SYTO 82 detection dye (Life Technologies #S11363) [ ]. .. Reactions were incubated at a constant temperature of 63°C for 50–70 minutes, with plate read steps at intervals of 1 minute (in the BioRad CFX96, this is accomplished with a 48-second single-temperature cycle followed by a plate read which takes approximately 12 seconds in all-channel mode).

    other:

    Article Title: Single Cell Transcriptome Amplification with MALBAC
    Article Snippet: Reagents list M2 medium (Sigma-Aldrich, cat. no. M7167-100ML) Fetal calf serum (Fisher Scientific, cat. no. R92157) Phosphate buffered saline (PBS), 1x (Thermo Scientific, cat. no. SH30256.01) Dispase (BD Biosciences, cat. no. 354235) Leibovitz’s L-15 medium (ATCC, cat. no. 30–2008) Fetal bovine serum (ATCC, cat. no. 30–2020) Penicillin-Streptomycin, 100x (Mediatech, Inc., cat. no. 30-001-CI) Trypsin-EDTA, 0.25% (Mediatech, Inc., cat. no. 25-053-CI) Nuclease-free water (Ambion, cat. no. AM9937) Dithiothreitol (DTT), 1M (Life Technologies, cat. no. P2325) Superscript III Reverse Transcriptase (Life Technologies, cat. no. 18080–044) First-strand buffer, 5x (Life Technologies, cat. no. 18080–044) dNTP Mix, 10mM each (New England Bioloabs, Inc., cat. no. N0447L) IGEPAL CA-630 (Sigma-Aldrich, cat. no. I8896-50ML) RNase inhibitor (40U/μL) (Life Technologies, cat. no. AM2682) SUPERase-In (20U/μL) (Life Technologies, cat. no. cat. no. AM2694) T4 gene 32 protein (New England Bioloabs, Inc., cat. no. M0300L) ThermoPol reaction buffer, 10x (New England Bioloabs, Inc., cat. no. B9004S) Magnesium Sulfate (MgSO4 ) Solution (100 mM) (New England Bioloabs, Inc., cat. no. B1003S) Deep-ventR (exo-) DNA Polymerase (2,000 U/mL) (New England Bioloabs, Inc., cat. no. M0259L) DNA clean & concentrator-5 (Zymo Research, cat. no. D4013)

    Article Title: Controllable Autocatalytic Cleavage-Mediated Fluorescence Recovery for Homogeneous Sensing of Alkyladenine DNA Glycosylase from Human Cancer Cells
    Article Snippet: Chemicals and materials Human alkyladenine DNA glycosylase (hAAG), 10× ThermoPol reaction buffer (200 mM trizma hydrochloride (Tris-HCl), 100 mM ammonium sulfate ((NH4 )2 SO4 ), 100 mM potassium chloride (KCl), 20 mM magnesium sulfate (MgSO4 ), 1% Triton X-100, pH 8.8), human apurinic/apyrimidinic endonuclease (APE1), 10× NEBuffer 4 (500 mM potassium acetate (KAc), 200 mM tris-acetate (Tris-Ac), 100 mM magnesium acetate (Mg(Ac)2 ), 10 mM DL-Dithiothreitol (DTT), pH 7.9), T7 exonuclease (T7 exo), and uracil-DNA glycosylase (UDG) were purchased from New England BioLabs (Beverly, MA, USA).

    Stripping Membranes:

    Article Title: Surveillance for Western Equine Encephalitis, St. Louis Encephalitis, and West Nile Viruses Using Reverse Transcription Loop-Mediated Isothermal Amplification
    Article Snippet: RT-LAMP RT-LAMP was performed in 10 μL reaction volumes in thin-walled PCR strip tubes or 96-well plates. .. The reaction mixture had a final composition (after adding water or template) of 1X Isothermal Amplification Buffer (New England Biolabs, NEB #B0537S) supplemented with an additional 6 mM MgSO4 (NEB #B1003S, final 8 mM MgSO4 ), 0.8 M Betaine (Sigma #B-0300), 1.4 mM each dNTP (NEB #N0447L), 0.32 units/μL Bst 2.0 WarmStart DNA polymerase (NEB #M0538M), 0.2 units/μL AMV reverse transcriptase (NEB #M0277T, or Life Science Advanced Technologies #AMVRTT-5), and 2 μM (or in some instances 4 μM) SYTO 82 detection dye (Life Technologies #S11363) [ ].

    Polymerase Chain Reaction:

    Article Title: Surveillance for Western Equine Encephalitis, St. Louis Encephalitis, and West Nile Viruses Using Reverse Transcription Loop-Mediated Isothermal Amplification
    Article Snippet: RT-LAMP RT-LAMP was performed in 10 μL reaction volumes in thin-walled PCR strip tubes or 96-well plates. .. The reaction mixture had a final composition (after adding water or template) of 1X Isothermal Amplification Buffer (New England Biolabs, NEB #B0537S) supplemented with an additional 6 mM MgSO4 (NEB #B1003S, final 8 mM MgSO4 ), 0.8 M Betaine (Sigma #B-0300), 1.4 mM each dNTP (NEB #N0447L), 0.32 units/μL Bst 2.0 WarmStart DNA polymerase (NEB #M0538M), 0.2 units/μL AMV reverse transcriptase (NEB #M0277T, or Life Science Advanced Technologies #AMVRTT-5), and 2 μM (or in some instances 4 μM) SYTO 82 detection dye (Life Technologies #S11363) [ ].

    Article Title: Generalized stochastic profiling of transcriptional regulatory heterogeneities in tissues, tumors, and cultured cells
    Article Snippet: Terminal transferase (Roche #03333574001, 400 U μl−1 ) 10× ThermoPol buffer (New England Biolabs #B9005S) 100 mM MgSO4 (New England Biolabs #B1003S) BSA (Roche #10711454001) Roche Taq polymerase (Roche #04728858001, 5 U μl−1 ) AL1 primer (200 nmol synthesis from IDT or MWG): ATTGGATCCAGGCCGCTCTGGACAAAATATGAATTCTTTTTTTTTTTTTTTTTTTTT TTT ▲ CRITICAL Long desalted primers will have varying purities depending on the manufacturer. .. 100× SYBR Green I (Invitrogen #S7563 diluted 100-fold in DMSO to 100×) 50 mM aminoallyl-dUTP (Ambion #1103015) High-Fidelity polymerase (Roche #11732650001, 3.5 U μl−1 ) 10× High-Fidelity PCR buffer without Mg2+ (included with Roche #11759175001) PureLink PCR Purification Kit (Invitrogen #46-6056) NaOAc (Calbiochem #567418) 20 mg/ml glycogen (Invitrogen #10814-010) NaHCO3 (Acros #21712500) Alexa Fluor 555 reactive dye decapack (Invitrogen # ) GEX hybridization buffer (included with Illumina #BD-103-0204 or equivalent)

    Hybridization:

    Article Title: Generalized stochastic profiling of transcriptional regulatory heterogeneities in tissues, tumors, and cultured cells
    Article Snippet: Terminal transferase (Roche #03333574001, 400 U μl−1 ) 10× ThermoPol buffer (New England Biolabs #B9005S) 100 mM MgSO4 (New England Biolabs #B1003S) BSA (Roche #10711454001) Roche Taq polymerase (Roche #04728858001, 5 U μl−1 ) AL1 primer (200 nmol synthesis from IDT or MWG): ATTGGATCCAGGCCGCTCTGGACAAAATATGAATTCTTTTTTTTTTTTTTTTTTTTT TTT ▲ CRITICAL Long desalted primers will have varying purities depending on the manufacturer. .. 100× SYBR Green I (Invitrogen #S7563 diluted 100-fold in DMSO to 100×) 50 mM aminoallyl-dUTP (Ambion #1103015) High-Fidelity polymerase (Roche #11732650001, 3.5 U μl−1 ) 10× High-Fidelity PCR buffer without Mg2+ (included with Roche #11759175001) PureLink PCR Purification Kit (Invitrogen #46-6056) NaOAc (Calbiochem #567418) 20 mg/ml glycogen (Invitrogen #10814-010) NaHCO3 (Acros #21712500) Alexa Fluor 555 reactive dye decapack (Invitrogen # ) GEX hybridization buffer (included with Illumina #BD-103-0204 or equivalent)

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    New England Biolabs magnesium sulfate mgso4 solution
    Magnesium Sulfate Mgso4 Solution, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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