mgso4  (New England Biolabs)


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    Name:
    Magnesium Sulfate MgSO4 Solution
    Description:
    Magnesium Sulfate MgSO4 Solution 6 0 ml
    Catalog Number:
    B1003S
    Price:
    23
    Category:
    General Chemical Solutions
    Size:
    6 0 ml
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    Structured Review

    New England Biolabs mgso4
    Magnesium Sulfate MgSO4 Solution
    Magnesium Sulfate MgSO4 Solution 6 0 ml
    https://www.bioz.com/result/mgso4/product/New England Biolabs
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mgso4 - by Bioz Stars, 2021-06
    98/100 stars

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    Related Articles

    Amplification:

    Article Title: Point-of-care testing for COVID-19 using SHERLOCK diagnostics
    Article Snippet: .. For Step (2) - STOPCovid detection reaction: - Bst 2.0 WarmStart® DNA Polymerase (M0538L), New England BioLabs - WarmStart® RTx Reverse Transcriptase (M0380L), New England BioLabs - 10X Isothermal Amplification Buffer (B0374S ), New England BioLabs, supplied with M0538L and M0380L - 100 mM MgSO 4 ( B1003S ), New England BioLabs, supplied with M0538L and M0380L - 10 mM Deoxynucleotide (dNTP) Solution Mix (N0447L ), New England BioLabs - Taurine (86329-100G ), Millipore Sigma - AapCas12b protein purified according to Kellner et al., Nature Protocols 2019, stored as 10 μL aliquots at 2 mg/mL. .. - Guide RNA for detecting N gene can be ordered from Synthego - Reporter DNA for lateral flow read out (Lateral Flow Reporter: /56-FAM/TTTTTTT/3Bio/), can be ordered from IDT - (Optional) Proteinase K Inhibitor (539470-10MG ), Millipore Sigma.

    Article Title: Real-Time, Digital LAMP with Commercial Microfluidic Chips Reveals the Interplay of Efficiency, Speed, and Background Amplification as a Function of Reaction Temperature and Time
    Article Snippet: Primers: 1.6 μM FIP/BIP, 0.2 μM FOP/BOP, and 0.4 μM LoopF/LoopB, 1 mg/mL BSA (New England BioLabs, ref B90005), 320 U/mL Bst 3.0, Ambion RNase cocktail (ThermoFisher, Waltham, MA, USA; ref AM2286, 5 U/mL RNase A, 400 U/mL TNase T1), 2 μM SYTO 9 (ThermoFisher, ref S34854), and approximately 660 copies/μL template in Ambion nuclease-free water (ThermoFisher, ref AM9932). .. LAMP experiments using Bst 2.0 ( a,c,e,g) contained the following final concentrations, optimized as shown in Supporting Information, Figure S3 : 1× Isothermal Amplification Buffer (New England BioLabs; ref B0537S, containing 20 mM Tris-HCl 10 mM (NH4 )2 SO4 , 50 mM KCl, 2 mM MgSO4 , 0.1% Tween 20 pH 8.8 at 25 °C), additional 6 mM MgSO4 (New England BioLabs; ref B1003S), 1.4 mM Deoxynucleotide Solution Mix (New England BioLabs; ref N0447S). .. Primers: 1.6 μM FIP/BIP, 0.2 μM FOP/BOP, and 0.4 μM LoopF/LoopB, 1 mg/mL BSA (New England BioLabs; ref B90005), 320 U/mL Bst 2.0 (New England BioLabs; ref M0537S), Ambion RNase cocktail (ThermoFisher, ref AM2286, 5 U/mL RNase A, 400 U/mL TNase T1), 2 μM SYTO 9 (ThermoFisher, ref S34854), and approximately 660 copies/μL template in Ambion nuclease-free water (ThermoFisher; ref AM9932).

    Article Title: Bisulfite-free epigenomics and genomics of single cells through methylation-sensitive restriction
    Article Snippet: Ten cycles (K_01–K_07) or eight cycles (K_08–K_27 and O_01–O_20) of quasilinear amplification were performed (25 °C for 2 min, 30 °C for 50 s, 40 °C for 45 s, 50 °C for 45 s, 70 °C for 2 min, 92 °C for 30 s, and 64 °C for 20 s). .. After quasilinear amplification, samples were further PCR-amplified by addition of 16 µl PCR mix: 1.6 µl 10× ThermoPol II Mg-free Reaction Buffer (NEB), 0.4 µl dNTP Solution Mix (10 mM each nt; NEB), 2.7 µl 10 µM GAT primer, 0.34 µl 100 mM MgSO4 Solution (NEB), 0.2 µl Deep Vent (exo-) DNA Polymerase (2 units/µl; NEB), and 10.76 µl water. .. The final sample volume of 34 μl was incubated in a PCR cycler with the following program: initial denaturation at 95 °C for 1 min, 19 cycles (K_01–K_07) or 16 cycles (K_08–K_27 and O_01–O_20) of 95 °C for 20 s, 60 °C for 30 s, and 72 °C for 4 min; final extension at 72 °C for 5 min (100 °C lid temperature).

    Purification:

    Article Title: Point-of-care testing for COVID-19 using SHERLOCK diagnostics
    Article Snippet: .. For Step (2) - STOPCovid detection reaction: - Bst 2.0 WarmStart® DNA Polymerase (M0538L), New England BioLabs - WarmStart® RTx Reverse Transcriptase (M0380L), New England BioLabs - 10X Isothermal Amplification Buffer (B0374S ), New England BioLabs, supplied with M0538L and M0380L - 100 mM MgSO 4 ( B1003S ), New England BioLabs, supplied with M0538L and M0380L - 10 mM Deoxynucleotide (dNTP) Solution Mix (N0447L ), New England BioLabs - Taurine (86329-100G ), Millipore Sigma - AapCas12b protein purified according to Kellner et al., Nature Protocols 2019, stored as 10 μL aliquots at 2 mg/mL. .. - Guide RNA for detecting N gene can be ordered from Synthego - Reporter DNA for lateral flow read out (Lateral Flow Reporter: /56-FAM/TTTTTTT/3Bio/), can be ordered from IDT - (Optional) Proteinase K Inhibitor (539470-10MG ), Millipore Sigma.

    Fluorescence:

    Article Title: Contamination-free visual detection of CaMV35S promoter amplicon using CRISPR/Cas12a coupled with a designed reaction vessel: Rapid, specific and sensitive.
    Article Snippet: An ultrafast and convenient method for visually detecting CaMV35S promoter amplicon (amplified products) was established by using CRISPR/Cas12a system coupled with a designed reaction vessel. .. An ultrafast and convenient method for visually detecting CaMV35S promoter amplicon (amplified products) was established by using CRISPR/Cas12a system coupled with a designed reaction vessel. .. An ultrafast and convenient method for visually detecting CaMV35S promoter amplicon (amplified products) was established by using CRISPR/Cas12a system coupled with a designed reaction vessel.

    Concentration Assay:

    Article Title: Contamination-free visual detection of CaMV35S promoter amplicon using CRISPR/Cas12a coupled with a designed reaction vessel: Rapid, specific and sensitive.
    Article Snippet: An ultrafast and convenient method for visually detecting CaMV35S promoter amplicon (amplified products) was established by using CRISPR/Cas12a system coupled with a designed reaction vessel. .. An ultrafast and convenient method for visually detecting CaMV35S promoter amplicon (amplified products) was established by using CRISPR/Cas12a system coupled with a designed reaction vessel. .. An ultrafast and convenient method for visually detecting CaMV35S promoter amplicon (amplified products) was established by using CRISPR/Cas12a system coupled with a designed reaction vessel.

    CRISPR:

    Article Title: Contamination-free visual detection of CaMV35S promoter amplicon using CRISPR/Cas12a coupled with a designed reaction vessel: Rapid, specific and sensitive.
    Article Snippet: An ultrafast and convenient method for visually detecting CaMV35S promoter amplicon (amplified products) was established by using CRISPR/Cas12a system coupled with a designed reaction vessel. .. An ultrafast and convenient method for visually detecting CaMV35S promoter amplicon (amplified products) was established by using CRISPR/Cas12a system coupled with a designed reaction vessel. .. An ultrafast and convenient method for visually detecting CaMV35S promoter amplicon (amplified products) was established by using CRISPR/Cas12a system coupled with a designed reaction vessel.

    other:

    Article Title: Controllable Autocatalytic Cleavage-Mediated Fluorescence Recovery for Homogeneous Sensing of Alkyladenine DNA Glycosylase from Human Cancer Cells
    Article Snippet: Chemicals and materials Human alkyladenine DNA glycosylase (hAAG), 10× ThermoPol reaction buffer (200 mM trizma hydrochloride (Tris-HCl), 100 mM ammonium sulfate ((NH4 )2 SO4 ), 100 mM potassium chloride (KCl), 20 mM magnesium sulfate (MgSO4 ), 1% Triton X-100, pH 8.8), human apurinic/apyrimidinic endonuclease (APE1), 10× NEBuffer 4 (500 mM potassium acetate (KAc), 200 mM tris-acetate (Tris-Ac), 100 mM magnesium acetate (Mg(Ac)2 ), 10 mM DL-Dithiothreitol (DTT), pH 7.9), T7 exonuclease (T7 exo), and uracil-DNA glycosylase (UDG) were purchased from New England BioLabs (Beverly, MA, USA).

    Article Title: Single Cell Transcriptome Amplification with MALBAC
    Article Snippet: Reagents list M2 medium (Sigma-Aldrich, cat. no. M7167-100ML) Fetal calf serum (Fisher Scientific, cat. no. R92157) Phosphate buffered saline (PBS), 1x (Thermo Scientific, cat. no. SH30256.01) Dispase (BD Biosciences, cat. no. 354235) Leibovitz’s L-15 medium (ATCC, cat. no. 30–2008) Fetal bovine serum (ATCC, cat. no. 30–2020) Penicillin-Streptomycin, 100x (Mediatech, Inc., cat. no. 30-001-CI) Trypsin-EDTA, 0.25% (Mediatech, Inc., cat. no. 25-053-CI) Nuclease-free water (Ambion, cat. no. AM9937) Dithiothreitol (DTT), 1M (Life Technologies, cat. no. P2325) Superscript III Reverse Transcriptase (Life Technologies, cat. no. 18080–044) First-strand buffer, 5x (Life Technologies, cat. no. 18080–044) dNTP Mix, 10mM each (New England Bioloabs, Inc., cat. no. N0447L) IGEPAL CA-630 (Sigma-Aldrich, cat. no. I8896-50ML) RNase inhibitor (40U/μL) (Life Technologies, cat. no. AM2682) SUPERase-In (20U/μL) (Life Technologies, cat. no. cat. no. AM2694) T4 gene 32 protein (New England Bioloabs, Inc., cat. no. M0300L) ThermoPol reaction buffer, 10x (New England Bioloabs, Inc., cat. no. B9004S) Magnesium Sulfate (MgSO4 ) Solution (100 mM) (New England Bioloabs, Inc., cat. no. B1003S) Deep-ventR (exo-) DNA Polymerase (2,000 U/mL) (New England Bioloabs, Inc., cat. no. M0259L) DNA clean & concentrator-5 (Zymo Research, cat. no. D4013)

    Gel Permeation Chromatography:

    Article Title: Single-ribonucleotide repair-mediated ligation-dependent cycling signal amplification for sensitive and specific detection of DNA methyltransferase repair-mediated ligation-dependent cycling signal amplification for sensitive and specific detection of DNA methyltransferase †Electronic supplementary
    Article Snippet: All oligonucleotides ( ) were synthesized and purified by HPLC at Shanghai Sangon Biological Engineering Technology & Services Co. Ltd. (Shanghai, China). .. DNA adenine methyltransferase (Dam MTase), 10× Dam MTase reaction buffer (500 mM trizma hydrochloride (Tris–HCl), 50 mM 2-mercaptoethanol (β-ME), 100 mM ethylenediaminetetraacetic acid (EDTA), pH 7.5), CpG methyltransferase (M.SssI), GpC methyltransferase (M.CviPI), Dpn I, 10× CutSmart buffer (500 mM potassium acetate (KAc), 200 mM tris-acetate, 100 mM magnesium acetate (Mg(Ac)2 ), 1 mg mL–1 bovine serum albumin (BSA), pH 7.9), S -adenosylmethionine (SAM), Taq DNA ligase, 10× Taq DNA ligase reaction buffer (200 mM Tris–HCl, 250 mM KAc, 100 mM Mg(Ac)2 , 100 mM DL-dithiothreitol (DTT), 10 mM nicotinamide adenine dinucleotide (NAD), 1% triton X-100, pH 7.6), Bst DNA polymerase (large fragment), 10× ThermoPol reaction buffer (200 mM Tris–HCl, 100 mM potassium chloride (KCl), 100 mM ammonium sulfate (NH4 )2 SO4 ), 20 mM magnesium sulfate (MgSO4 ), 1% triton X-100, pH 8.8), ribonuclease HII (RNase HII), deoxyadenosine triphosphate (dATP), deoxyuridine triphosphate (dUTP), deoxyguanosine triphosphate (dGTP) and deoxycytidine triphosphate (dCTP) were purchased from New England Biolabs (Ipswich, MA, USA). .. SYBR Gold was purchased from Life Technologies (Carlsbad, CA, USA).

    Polymerase Chain Reaction:

    Article Title: Bisulfite-free epigenomics and genomics of single cells through methylation-sensitive restriction
    Article Snippet: Ten cycles (K_01–K_07) or eight cycles (K_08–K_27 and O_01–O_20) of quasilinear amplification were performed (25 °C for 2 min, 30 °C for 50 s, 40 °C for 45 s, 50 °C for 45 s, 70 °C for 2 min, 92 °C for 30 s, and 64 °C for 20 s). .. After quasilinear amplification, samples were further PCR-amplified by addition of 16 µl PCR mix: 1.6 µl 10× ThermoPol II Mg-free Reaction Buffer (NEB), 0.4 µl dNTP Solution Mix (10 mM each nt; NEB), 2.7 µl 10 µM GAT primer, 0.34 µl 100 mM MgSO4 Solution (NEB), 0.2 µl Deep Vent (exo-) DNA Polymerase (2 units/µl; NEB), and 10.76 µl water. .. The final sample volume of 34 μl was incubated in a PCR cycler with the following program: initial denaturation at 95 °C for 1 min, 19 cycles (K_01–K_07) or 16 cycles (K_08–K_27 and O_01–O_20) of 95 °C for 20 s, 60 °C for 30 s, and 72 °C for 4 min; final extension at 72 °C for 5 min (100 °C lid temperature).

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  • 98
    New England Biolabs magnesium sulfate mgso4 solution
    Magnesium Sulfate Mgso4 Solution, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/magnesium sulfate mgso4 solution/product/New England Biolabs
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    magnesium sulfate mgso4 solution - by Bioz Stars, 2021-06
    98/100 stars
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