isothermal amplification buffer  (New England Biolabs)


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    Name:
    Isothermal Amplification Buffer
    Description:
    Isothermal Amplification Buffer 6 0 ml
    Catalog Number:
    b0537s
    Price:
    28
    Size:
    6 0 ml
    Category:
    Buffers
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    New England Biolabs isothermal amplification buffer
    Isothermal Amplification Buffer
    Isothermal Amplification Buffer 6 0 ml
    https://www.bioz.com/result/isothermal amplification buffer/product/New England Biolabs
    Average 99 stars, based on 78 article reviews
    Price from $9.99 to $1999.99
    isothermal amplification buffer - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    SYBR Green Assay:

    Article Title: Direct and Rapid Detection and Quantification of Oenococcus oeni Cells in Wine by Cells-LAMP and Cells-qLAMP
    Article Snippet: .. Each reaction contained 1.4 mM of dNTPs, 0.2 μM of each outer primer, 1.6 μM of each inner primer, 0.8 μM of each loop primer, 8 mM of MgCl2 (Invitrogen), 1× Isothermal Amplification Buffer, 0.4 U/μL of Bst polymerase 2.0 (New England BioLabs), 11.5 μL of the mechanically lysed cells, and 0.4 μM of SYTO-9 (Invitrogen) or 1 μL of 10× SYBR Green I (Invitrogen). .. The qLAMP amplifications were performed in triplicate in a C100TM Thermal Cycler, CFX96TM Real-Time System (BioRad), which operated at a constant temperature of 62°C for 1 h. Fluorescence signals were collected every minute, followed by a melting curve analysis obtained by slow heating from 60°C to 95°C at 0.5°C every 5 s, with continuous fluorescence collection.

    Staining:

    Article Title: A smartphone-based diagnostic platform for rapid detection of Zika, chikungunya, and dengue viruses
    Article Snippet: .. The fresh reaction mixture contained 1x Isothermal Amplification Buffer (20 mM Tris-HCl, 10 mM (NH4 )2SO4 , 50 mM KCl, 2 mM MgSO4 , 0.1% Tween® 20, pH 8.8@25 °C, NEB), 700 mM of Betaine (Sigma), 0.14 mM of each nucleotide, 8 mM MgSO4 , 2 μM SYTO® 9 Stain (Thermo Fisher Scientific), 2 units of Porcine RNase Inhibitor , 0.2 μM each F3 and B3, 0.8 μM each LF and LB, 1.6 μM each FIP and BIP primers , 3.2 units of avian myeloblastosis virus (AMV) reverse transcriptase (Life Sciences Advanced Technologies Inc., St Petersburg, FL), and 3.2 units of Bst 2.0 WarmStart® DNA Polymerase (NEB). .. To make the dried reagent formulation, isothermal amplification buffer, betaine and magnesium sulfate were removed.

    Amplification:

    Article Title: Development of loop-mediated isothermal amplification (LAMP) for simple detection of Leishmania infection
    Article Snippet: .. Loop-mediated isothermal amplification The LAMP reaction mixtures (25 μl) were based on that described by Tomita et al. [ ], which contained 1× Isothermal Amplification Buffer (New England Biolabs, USA), 8 mM MgSO4 , 0.8 M Betaine (Sigma-Aldrich, USA), 1.4 mM each of dATP, dCTP, dGTP, and dTTP (SibEnzyme, Russia), 40 pmol of FIP primer, 40 pmol of BIP primer, 10 pmol of F3 primer, 10 pmol of B3 primer, and 8 U of Bst 2.0 WarmStart® DNA Polymerase (New England Biolabs, USA). .. In addition, the colorimetric indicator, 0.008 % MG (Sigma-Aldrich, USA) was added.

    Article Title: Development of a loop-mediated isothermal amplification (LAMP) assay for rapid screening of ticks and fleas for spotted fever group rickettsia
    Article Snippet: .. Bst 2.0 LAMP Each LAMP reaction totaled 25 μl consisting of 9 μl nuclease free water, 2.5 μl 10x Isothermal Amplification Buffer Pack (contains 20mM Tris-HCl, 10mM (NH4 )2 SO4 , 50mM KCl, 2mM MgSO4 , 0.1% Tween® 20) (New England Biolabs), 3.5 μl 10 mM each dNTPs (New England Biolabs), 1 μl 100 mM MgSO4 (New England Biolabs), 3.5 μl 5M Betaine (Affymetrix), and 2.5 μl 10x primer mix (2μM F3 primer, 2μM B3 primer, 16μM FIP, 16μM BIP, 8μM LoopF and 8μM LoopB), 1.0 μl Bst 2.0 WarmStart DNA Polymerase (8 units/μl) (New England Biolabs), 1.0 μl 3mM Hydroxynaphthol blue (HNB) (Sigma-Aldrich), and 1 μl of sample DNA. ..

    Article Title: Visual Detection of Bacterial Pathogens via PNA-Based Padlock Probe Assembly and Isothermal Amplification of DNAzymes
    Article Snippet: .. The ELRCA reaction was performed with 100pM of the circular template, 8 units of Bst2.0 DNA Polymerase (obtained from New England Biolabs), 1× Isothermal Amplification Buffer (obtained from New England Biolabs), 1 mM dNTPs, and 1 μM of each primer. .. Limit of Detection Assay A synthetic target template was used to ligate the ODN probe and evaluate the limit of detection.

    Article Title: Integrated LAMP and immunoassay platform for diarrheal disease detection
    Article Snippet: .. Reactions consist of 1 μL of target sample in 9 μL of LAMP reaction mix containing 1× Isothermal Amplification Buffer (20 mM Tris-HCl, 10 mM (NH4 )2 SO4 , 50 mM KCl, 2 mM MgSO4 , 0.1% Tween 20, pH 8.8@25°C) (NEB), 800 mM of Betaine (Sigma), 0.14 mM of each nucleotide, 6 mM MgSO4 , 0.2 μM each F3 and B3, 0.8 μM each LF and LB, 1.6 μM each FIP and BIP, 1.6 μM quencher primers ( , see Supplementary material), and 3.2 units of Bst 2.0 WarmStart DNA Polymerase (NEB). ..

    Article Title: A field survey using LAMP assay for detection of Schistosoma mansoni in a low-transmission area of schistosomiasis in Umbuzeiro, Brazil: Assessment in human and snail samples
    Article Snippet: .. Briefly, the reaction was carried out in 25 μL reaction mixture containing 40 pmol each of FIP and BIP primers, 5 pmol of each F3 and B3 primers, 1.4 mM of each dNTP (Intron), 1x Isothermal Amplification Buffer -20 mM Tris-HCl (pH 8.8), 50 mM KCl, 10 mM (NH4 )2 SO4 , 2 mM MgSO4 , 0.1% Tween20- (New England Biolabs, UK), 1 M betaine (Sigma, USA), supplementary 6 mM of MgSO4 (New England Biolabs, UK) and 8 U of Bst 2.0 WarmStart DNA polymerase (New England Biolabs, UK) with 2 μL of template DNA. .. Reaction tubes were placed in a heating block at a constant temperature of 63°C for 60 min and then heated at 80°C for 5 min to stop the reaction.

    Article Title: A Loop-Mediated Isothermal Amplification (LAMP) Assay for Early Detection of Schistosoma mansoni in Stool Samples: A Diagnostic Approach in a Murine Model
    Article Snippet: .. Thus, LAMP reactions mixtures (25 µL) contained 40 pmol of each FIP and BIP primers, 5 pmol of each F3 and B3 primers, 1.4 mM of each dNTP (Bioron), 1× Isothermal Amplification Buffer −20 mM Tris-HCl (pH 8.8), 50 mM KCl, 10 mM (NH4 )2 SO4 , 2 mM MgSO4 , 0.1% Tween20- (New England Biolabs, UK), betaine (ranging 0.8, 1, 1.2, 1.4 or 1.6 M) (Sigma, USA), supplementary MgSO4 (ranging 2, 4, 6 or 8 mM) (New England Biolabs, UK) and 8 U of Bst 2.0 WarmStart DNA polymerase (New England Biolabs, UK) with 2 µL of template DNA. .. To establish the standard protocol for the two LAMP reaction mixtures assayed, different temperatures were tested using a heating block (K Dry-Bath) set at 61, 63 and 65°C for 60 min and then heated at 80°C for 5 min to terminate the reaction.

    Article Title: A smartphone-based diagnostic platform for rapid detection of Zika, chikungunya, and dengue viruses
    Article Snippet: .. The fresh reaction mixture contained 1x Isothermal Amplification Buffer (20 mM Tris-HCl, 10 mM (NH4 )2SO4 , 50 mM KCl, 2 mM MgSO4 , 0.1% Tween® 20, pH 8.8@25 °C, NEB), 700 mM of Betaine (Sigma), 0.14 mM of each nucleotide, 8 mM MgSO4 , 2 μM SYTO® 9 Stain (Thermo Fisher Scientific), 2 units of Porcine RNase Inhibitor , 0.2 μM each F3 and B3, 0.8 μM each LF and LB, 1.6 μM each FIP and BIP primers , 3.2 units of avian myeloblastosis virus (AMV) reverse transcriptase (Life Sciences Advanced Technologies Inc., St Petersburg, FL), and 3.2 units of Bst 2.0 WarmStart® DNA Polymerase (NEB). .. To make the dried reagent formulation, isothermal amplification buffer, betaine and magnesium sulfate were removed.

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  • 99
    New England Biolabs isothermal amplification buffer
    Isothermal Amplification Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isothermal amplification buffer/product/New England Biolabs
    Average 99 stars, based on 59 article reviews
    Price from $9.99 to $1999.99
    isothermal amplification buffer - by Bioz Stars, 2020-07
    99/100 stars
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