isothermal amplification buffer  (New England Biolabs)


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    Name:
    Isothermal Amplification Buffer
    Description:
    Isothermal Amplification Buffer 6 0 ml
    Catalog Number:
    b0537s
    Price:
    28
    Size:
    6 0 ml
    Category:
    Buffers
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    Structured Review

    New England Biolabs isothermal amplification buffer
    Isothermal Amplification Buffer
    Isothermal Amplification Buffer 6 0 ml
    https://www.bioz.com/result/isothermal amplification buffer/product/New England Biolabs
    Average 99 stars, based on 80 article reviews
    Price from $9.99 to $1999.99
    isothermal amplification buffer - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Modification:

    Article Title: A hydrogel beads based platform for single-cell phenotypic analysis and digital molecular detection
    Article Snippet: .. Each 20 μL of modified LAMP mix for digital single bacteria LAMP contained 1× isothermal buffer, 6 mM total MgSO4, 1.4 mM dNTP, 640 U/mL Bst 2.0 WarmStart polymerase, 1.6 μM FIB and BIP, 0.2 μM F3 and B3, 0.8 μM LF and LB, 1.5 mg/mL BSA, 1× LAMP dye ( ). ..

    Staining:

    Article Title: A smartphone-based diagnostic platform for rapid detection of Zika, chikungunya, and dengue viruses
    Article Snippet: .. The fresh reaction mixture contained 1x Isothermal Amplification Buffer (20 mM Tris-HCl, 10 mM (NH4 )2SO4 , 50 mM KCl, 2 mM MgSO4 , 0.1% Tween® 20, pH 8.8@25 °C, NEB), 700 mM of Betaine (Sigma), 0.14 mM of each nucleotide, 8 mM MgSO4 , 2 μM SYTO® 9 Stain (Thermo Fisher Scientific), 2 units of Porcine RNase Inhibitor , 0.2 μM each F3 and B3, 0.8 μM each LF and LB, 1.6 μM each FIP and BIP primers , 3.2 units of avian myeloblastosis virus (AMV) reverse transcriptase (Life Sciences Advanced Technologies Inc., St Petersburg, FL), and 3.2 units of Bst 2.0 WarmStart® DNA Polymerase (NEB). .. To make the dried reagent formulation, isothermal amplification buffer, betaine and magnesium sulfate were removed.

    Amplification:

    Article Title: Development of loop-mediated isothermal amplification (LAMP) for simple detection of Leishmania infection
    Article Snippet: .. Loop-mediated isothermal amplification The LAMP reaction mixtures (25 μl) were based on that described by Tomita et al. [ ], which contained 1× Isothermal Amplification Buffer (New England Biolabs, USA), 8 mM MgSO4 , 0.8 M Betaine (Sigma-Aldrich, USA), 1.4 mM each of dATP, dCTP, dGTP, and dTTP (SibEnzyme, Russia), 40 pmol of FIP primer, 40 pmol of BIP primer, 10 pmol of F3 primer, 10 pmol of B3 primer, and 8 U of Bst 2.0 WarmStart® DNA Polymerase (New England Biolabs, USA). .. In addition, the colorimetric indicator, 0.008 % MG (Sigma-Aldrich, USA) was added.

    Article Title: Development of a loop-mediated isothermal amplification (LAMP) assay for rapid screening of ticks and fleas for spotted fever group rickettsia
    Article Snippet: .. Bst 2.0 LAMP Each LAMP reaction totaled 25 μl consisting of 9 μl nuclease free water, 2.5 μl 10x Isothermal Amplification Buffer Pack (contains 20mM Tris-HCl, 10mM (NH4 )2 SO4 , 50mM KCl, 2mM MgSO4 , 0.1% Tween® 20) (New England Biolabs), 3.5 μl 10 mM each dNTPs (New England Biolabs), 1 μl 100 mM MgSO4 (New England Biolabs), 3.5 μl 5M Betaine (Affymetrix), and 2.5 μl 10x primer mix (2μM F3 primer, 2μM B3 primer, 16μM FIP, 16μM BIP, 8μM LoopF and 8μM LoopB), 1.0 μl Bst 2.0 WarmStart DNA Polymerase (8 units/μl) (New England Biolabs), 1.0 μl 3mM Hydroxynaphthol blue (HNB) (Sigma-Aldrich), and 1 μl of sample DNA. ..

    Article Title: Programmable low-cost DNA-based platform for viral RNA detection
    Article Snippet: .. Isothermal amplification by NASBA First, we employed the classic NASBA protocol to prove the concept ( Fig. S15 ). .. The 25 μl one-pot reaction contained 3 μl RNA sample at various concentrations, 0.4 μM forward and reverse primers, 8 U AMV Reverse Transcriptase, 50 U T7 RNA Polymerase, 0.1 U RNase H, 40 U RNase Inhibitor (NEB, Murine), 2 mM NTP mix, 1 mM rNTP mix, 12 mM MgCl2, 40 mM Tris-HCl, 42 mM KCl, 5 mM Dithiotreitol (DTT), 15%(v/v) dimethyl sulfoxide.

    Article Title: Visual Detection of Bacterial Pathogens via PNA-Based Padlock Probe Assembly and Isothermal Amplification of DNAzymes
    Article Snippet: .. The ELRCA reaction was performed with 100pM of the circular template, 8 units of Bst2.0 DNA Polymerase (obtained from New England Biolabs), 1× Isothermal Amplification Buffer (obtained from New England Biolabs), 1 mM dNTPs, and 1 μM of each primer. .. Limit of Detection Assay A synthetic target template was used to ligate the ODN probe and evaluate the limit of detection.

    Article Title: Integrated LAMP and immunoassay platform for diarrheal disease detection
    Article Snippet: .. Reactions consist of 1 μL of target sample in 9 μL of LAMP reaction mix containing 1× Isothermal Amplification Buffer (20 mM Tris-HCl, 10 mM (NH4 )2 SO4 , 50 mM KCl, 2 mM MgSO4 , 0.1% Tween 20, pH 8.8@25°C) (NEB), 800 mM of Betaine (Sigma), 0.14 mM of each nucleotide, 6 mM MgSO4 , 0.2 μM each F3 and B3, 0.8 μM each LF and LB, 1.6 μM each FIP and BIP, 1.6 μM quencher primers ( , see Supplementary material), and 3.2 units of Bst 2.0 WarmStart DNA Polymerase (NEB). ..

    Article Title: A Loop-Mediated Isothermal Amplification (LAMP) Assay for Early Detection of Schistosoma mansoni in Stool Samples: A Diagnostic Approach in a Murine Model
    Article Snippet: .. Thus, LAMP reactions mixtures (25 µL) contained 40 pmol of each FIP and BIP primers, 5 pmol of each F3 and B3 primers, 1.4 mM of each dNTP (Bioron), 1× Isothermal Amplification Buffer −20 mM Tris-HCl (pH 8.8), 50 mM KCl, 10 mM (NH4 )2 SO4 , 2 mM MgSO4 , 0.1% Tween20- (New England Biolabs, UK), betaine (ranging 0.8, 1, 1.2, 1.4 or 1.6 M) (Sigma, USA), supplementary MgSO4 (ranging 2, 4, 6 or 8 mM) (New England Biolabs, UK) and 8 U of Bst 2.0 WarmStart DNA polymerase (New England Biolabs, UK) with 2 µL of template DNA. .. To establish the standard protocol for the two LAMP reaction mixtures assayed, different temperatures were tested using a heating block (K Dry-Bath) set at 61, 63 and 65°C for 60 min and then heated at 80°C for 5 min to terminate the reaction.

    Article Title: A smartphone-based diagnostic platform for rapid detection of Zika, chikungunya, and dengue viruses
    Article Snippet: .. The fresh reaction mixture contained 1x Isothermal Amplification Buffer (20 mM Tris-HCl, 10 mM (NH4 )2SO4 , 50 mM KCl, 2 mM MgSO4 , 0.1% Tween® 20, pH 8.8@25 °C, NEB), 700 mM of Betaine (Sigma), 0.14 mM of each nucleotide, 8 mM MgSO4 , 2 μM SYTO® 9 Stain (Thermo Fisher Scientific), 2 units of Porcine RNase Inhibitor , 0.2 μM each F3 and B3, 0.8 μM each LF and LB, 1.6 μM each FIP and BIP primers , 3.2 units of avian myeloblastosis virus (AMV) reverse transcriptase (Life Sciences Advanced Technologies Inc., St Petersburg, FL), and 3.2 units of Bst 2.0 WarmStart® DNA Polymerase (NEB). .. To make the dried reagent formulation, isothermal amplification buffer, betaine and magnesium sulfate were removed.

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  • 99
    New England Biolabs isothermal amplification buffer
    Isothermal Amplification Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isothermal amplification buffer/product/New England Biolabs
    Average 99 stars, based on 59 article reviews
    Price from $9.99 to $1999.99
    isothermal amplification buffer - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

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