Phusion Gc Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phusion gc buffer/product/New England Biolabs
Average 97 stars, based on 1 article reviews
Price from $9.99 to $1999.99
1) Product Images from "Restriction enzyme-free mutagenesis via the light regulation of DNA polymerization"
Article Title: Restriction enzyme-free mutagenesis via the light regulation of DNA polymerization
Journal: Nucleic Acids Research
Figure Legend Snippet: Effects of a caged thymidine nucleobase on the PCR. ( A ) PCR product using Phusion DNA Polymerase. Polymerization is halted in the presence of a caging group, which is confirmed using the truncated primer P3 that affords the same length product. ( B ) PCR product using Taq DNA polymerase. Polymerization is halted in the presence of a caging group, which is confirmed using the truncated primer P3 that affords the same length product.
Techniques Used: Polymerase Chain Reaction
2) Product Images from "High-throughput mutagenesis using a two-fragment PCR approach"
Article Title: High-throughput mutagenesis using a two-fragment PCR approach
Journal: Scientific Reports
Figure Legend Snippet: Agarose gel electrophoresis analysis of PCR fragments multiplied by Phusion High-Fidelity PCR Master Mix with HF Buffer and Phusion High-Fidelity PCR Master Mix with GC Buffer. Analysed PCRs are labelled as the mutated residue and the letter indicating whether the fragment is multiplied by mutation-specific forward (F) or reverse (R) primer. Expected PCR fragments are between 3000 and 4000 bp long. While using the PCR master mix with GC buffer gave expected fragments in all shown cases, using the Phusion High-Fidelity polymerase with HF buffer failed to multiply four fragments.
Techniques Used: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Mutagenesis