isothermal amplification buffer ii pack  (New England Biolabs)


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    New England Biolabs isothermal amplification buffer ii pack
    Isothermal Amplification Buffer Ii Pack, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isothermal amplification buffer ii pack/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    isothermal amplification buffer ii pack - by Bioz Stars, 2022-05
    94/100 stars

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    New England Biolabs pcr amplification
    Strong adamts9 expression in preovulatory follicular cells and developing embryos. ( A ) Expression of adamts9 determined by <t>RT-PCR.</t> Top panel is adamts9 transcript analyzed by RT-PCR (35 cycles), bottom panel is expression of a house keep gene, eukaryotic translation elongation factor 1 alpha 1a ( <t>eef1a1a</t> ). M. NEB 1 kb plus DNA ladder; 1. Ovary (fully grown immature follicles); 2. Preovulatory stage IVb follicles; 3. Ovulated stage V oocytes; 4. One cell stage embryos; 5. Four cell stage embryos; 6. Oblong stage embryos (~ 3.5 hpf, hours post fertilization); 7. Germ-ring stage embryos (~ 5.5 hpf); 8. Eight somite stage embryos (~ 11.5 hpf); 9. 24 hpf (hours post fertilization) embryos; 10. Two dpf (days post fertilization ) embryos; 11. Three dpf embryos; 12. Six dpf embryos; 13. Six wpf (weeks post fertilization) gonad. Please see Supplemental Figs. 3 and 4 for full agarose gel images. ( B ) Strong adamts9 expression in preovulatory follicles, low or no expression in immature follicles or ovulated oocytes determined by real-time quantitative PCR (qPCR). Stage I and III immature follicles, IVb preovulatory follicular cell enclosed oocytes and stage V ovulated oocytes were collected from mature AB wildtype females between 7–8:30 am (lights on 8:30am-10:30 pm). Results were presented as mean ± SEM (n = 5). Different letters above the error bars indicate that those groups are significantly different from each other at p
    Pcr Amplification, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr amplification/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcr amplification - by Bioz Stars, 2022-05
    86/100 stars
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    86
    New England Biolabs isothermal amplification
    Strong adamts9 expression in preovulatory follicular cells and developing embryos. ( A ) Expression of adamts9 determined by <t>RT-PCR.</t> Top panel is adamts9 transcript analyzed by RT-PCR (35 cycles), bottom panel is expression of a house keep gene, eukaryotic translation elongation factor 1 alpha 1a ( <t>eef1a1a</t> ). M. NEB 1 kb plus DNA ladder; 1. Ovary (fully grown immature follicles); 2. Preovulatory stage IVb follicles; 3. Ovulated stage V oocytes; 4. One cell stage embryos; 5. Four cell stage embryos; 6. Oblong stage embryos (~ 3.5 hpf, hours post fertilization); 7. Germ-ring stage embryos (~ 5.5 hpf); 8. Eight somite stage embryos (~ 11.5 hpf); 9. 24 hpf (hours post fertilization) embryos; 10. Two dpf (days post fertilization ) embryos; 11. Three dpf embryos; 12. Six dpf embryos; 13. Six wpf (weeks post fertilization) gonad. Please see Supplemental Figs. 3 and 4 for full agarose gel images. ( B ) Strong adamts9 expression in preovulatory follicles, low or no expression in immature follicles or ovulated oocytes determined by real-time quantitative PCR (qPCR). Stage I and III immature follicles, IVb preovulatory follicular cell enclosed oocytes and stage V ovulated oocytes were collected from mature AB wildtype females between 7–8:30 am (lights on 8:30am-10:30 pm). Results were presented as mean ± SEM (n = 5). Different letters above the error bars indicate that those groups are significantly different from each other at p
    Isothermal Amplification, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isothermal amplification/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    isothermal amplification - by Bioz Stars, 2022-05
    86/100 stars
      Buy from Supplier

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    Strong adamts9 expression in preovulatory follicular cells and developing embryos. ( A ) Expression of adamts9 determined by RT-PCR. Top panel is adamts9 transcript analyzed by RT-PCR (35 cycles), bottom panel is expression of a house keep gene, eukaryotic translation elongation factor 1 alpha 1a ( eef1a1a ). M. NEB 1 kb plus DNA ladder; 1. Ovary (fully grown immature follicles); 2. Preovulatory stage IVb follicles; 3. Ovulated stage V oocytes; 4. One cell stage embryos; 5. Four cell stage embryos; 6. Oblong stage embryos (~ 3.5 hpf, hours post fertilization); 7. Germ-ring stage embryos (~ 5.5 hpf); 8. Eight somite stage embryos (~ 11.5 hpf); 9. 24 hpf (hours post fertilization) embryos; 10. Two dpf (days post fertilization ) embryos; 11. Three dpf embryos; 12. Six dpf embryos; 13. Six wpf (weeks post fertilization) gonad. Please see Supplemental Figs. 3 and 4 for full agarose gel images. ( B ) Strong adamts9 expression in preovulatory follicles, low or no expression in immature follicles or ovulated oocytes determined by real-time quantitative PCR (qPCR). Stage I and III immature follicles, IVb preovulatory follicular cell enclosed oocytes and stage V ovulated oocytes were collected from mature AB wildtype females between 7–8:30 am (lights on 8:30am-10:30 pm). Results were presented as mean ± SEM (n = 5). Different letters above the error bars indicate that those groups are significantly different from each other at p

    Journal: Scientific Reports

    Article Title: Delay in primordial germ cell migration in adamts9 knockout zebrafish

    doi: 10.1038/s41598-021-88024-x

    Figure Lengend Snippet: Strong adamts9 expression in preovulatory follicular cells and developing embryos. ( A ) Expression of adamts9 determined by RT-PCR. Top panel is adamts9 transcript analyzed by RT-PCR (35 cycles), bottom panel is expression of a house keep gene, eukaryotic translation elongation factor 1 alpha 1a ( eef1a1a ). M. NEB 1 kb plus DNA ladder; 1. Ovary (fully grown immature follicles); 2. Preovulatory stage IVb follicles; 3. Ovulated stage V oocytes; 4. One cell stage embryos; 5. Four cell stage embryos; 6. Oblong stage embryos (~ 3.5 hpf, hours post fertilization); 7. Germ-ring stage embryos (~ 5.5 hpf); 8. Eight somite stage embryos (~ 11.5 hpf); 9. 24 hpf (hours post fertilization) embryos; 10. Two dpf (days post fertilization ) embryos; 11. Three dpf embryos; 12. Six dpf embryos; 13. Six wpf (weeks post fertilization) gonad. Please see Supplemental Figs. 3 and 4 for full agarose gel images. ( B ) Strong adamts9 expression in preovulatory follicles, low or no expression in immature follicles or ovulated oocytes determined by real-time quantitative PCR (qPCR). Stage I and III immature follicles, IVb preovulatory follicular cell enclosed oocytes and stage V ovulated oocytes were collected from mature AB wildtype females between 7–8:30 am (lights on 8:30am-10:30 pm). Results were presented as mean ± SEM (n = 5). Different letters above the error bars indicate that those groups are significantly different from each other at p

    Article Snippet: A set of PCR primers targeting 242 bp of coding region of eef1a1a (forward: 5′-AGTGTTGCCTTCGTCCCAAT-3′; Reverse:5′-CACACGACCCACAGGTACAG-3′) was used for PCR amplification.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Agarose Gel Electrophoresis, Real-time Polymerase Chain Reaction