isothermal amplification buffer ii  (New England Biolabs)


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    Name:
    Isothermal Amplification Buffer II Pack
    Description:
    Isothermal Amplification Buffer II Pack 6 0 ml
    Catalog Number:
    b0374s
    Price:
    27
    Size:
    6 0 ml
    Category:
    Buffers
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    New England Biolabs isothermal amplification buffer ii
    Isothermal Amplification Buffer II Pack
    Isothermal Amplification Buffer II Pack 6 0 ml
    https://www.bioz.com/result/isothermal amplification buffer ii/product/New England Biolabs
    Average 95 stars, based on 62 article reviews
    Price from $9.99 to $1999.99
    isothermal amplification buffer ii - by Bioz Stars, 2020-10
    95/100 stars

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    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: Visual and modular detection of pathogen nucleic acids with enzyme–DNA molecular complexes
    Article Snippet: .. This mixture was incubated with 8 units of Bst 2.0 DNA polymerase (New England Biolabs) in 1× isothermal amplification buffer II, 6 mM MgSO4 , 2.5 mM dNTPs, at 70 °C for 1 h. The amplification product was purified via PCR cleanup spin columns (New England Biolabs) and quantified through gel electrophoresis. ..

    Amplification:

    Article Title: Real-Time, Digital LAMP with Commercial Microfluidic Chips Reveals the Interplay of Efficiency, Speed, and Background Amplification as a Function of Reaction Temperature and Time
    Article Snippet: .. LAMP experiments using Bst 3.0 ( ; b,d,e,f,h–j; ) contained the following final concentrations, optimized previously: 1× Isothermal Amplification Buffer II (New England BioLabs (NEB), Ipswich, MA, USA; ref B0374S, containing 20 mM Tris-HCl 10 mM (NH4 )2 SO4 , 150 mM KCl, 2 mM MgSO4 , 0.1% Tween 20 pH 8.8 at 25 °C), 4 mM additional MgSO4 (beyond 2 mM from buffer), 1.4 mM Deoxynucleotide Solution Mix. .. Primers: 1.6 μM FIP/BIP, 0.2 μM FOP/BOP, and 0.4 μM LoopF/LoopB, 1 mg/mL BSA (New England BioLabs, ref B90005), 320 U/mL Bst 3.0, Ambion RNase cocktail (ThermoFisher, Waltham, MA, USA; ref AM2286, 5 U/mL RNase A, 400 U/mL TNase T1), 2 μM SYTO 9 (ThermoFisher, ref S34854), and approximately 660 copies/μL template in Ambion nuclease-free water (ThermoFisher, ref AM9932).

    Article Title: Real-Time, Digital LAMP with Commercial Microfluidic Chips Reveals the Interplay of Efficiency, Speed, and Background Amplification as a Function of Reaction Temperature and Time
    Article Snippet: .. Figure LAMP experiments using Bst 2.0 ( : 1× Isothermal Amplification Buffer (New England BioLabs; ref B0537S, containing 20 mM Tris-HCl 10 mM (NH4 )2 SO4 , 50 mM KCl, 2 mM MgSO4 , 0.1% Tween 20 pH 8.8 at 25 °C), additional 6 mM MgSO4 (New England BioLabs; ref B1003S), 1.4 mM Deoxynucleotide Solution Mix (New England BioLabs; ref N0447S). .. Primers: 1.6 μM FIP/BIP, 0.2 μM FOP/BOP, and 0.4 μM LoopF/LoopB, 1 mg/mL BSA (New England BioLabs; ref B90005), 320 U/mL Bst 2.0 (New England BioLabs; ref M0537S), Ambion RNase cocktail (ThermoFisher, ref AM2286, 5 U/mL RNase A, 400 U/mL TNase T1), 2 μM SYTO 9 (ThermoFisher, ref S34854), and approximately 660 copies/μL template in Ambion nuclease-free water (ThermoFisher; ref AM9932).

    Article Title: Development of Reverse Transcription Loop-Mediated Isothermal Amplification Assays Targeting SARS-CoV-2
    Article Snippet: .. LAMP and RT-LAMP Reaction LAMP reaction was performed with reaction mixture containing following components: 1.6 μmol/L FIP/BIP primers, 0.2 μmol/L F3/B3 primers, 0.4 μmol/L loop forward/loop backward primers, 1× Isothermal Amplification Buffer II [NEB; 20 mmol/L Tris-HCl, pH 8.8, 10 mmol/L (NH4 )2 SO4 , 150 mmol/L KCl, 2 mmol/L MgSO4 , and 0.1% Tween 20], 6 mmol/L MgSO4 (NEB; final, 8 mmol/L Mg2+ ), 1.4 mmol/L each dNTP (Enzynomics), 0.4 μmol/L SYTO-9 (Invitrogen), and 6 U Bst 3.0 DNA polymerase (NEB) in total 15-μL reaction volume. .. For RT-LAMP, 10 U of SuperScript IV Reverse Transcriptase (Invitrogen) was added.

    Article Title: Real-Time, Digital LAMP with Commercial Microfluidic Chips Reveals the Interplay of Efficiency, Speed, and Background Amplification as a Function of Reaction Temperature and Time
    Article Snippet: .. LAMP experiments using Bst 3.0 ( ; b,d,e,f,h–j; ) contained the following final concentrations, optimized previously: 1× Isothermal Amplification Buffer II (New England BioLabs (NEB), Ipswich, MA, USA; ref B0374S, containing 20 mM Tris-HCl 10 mM (NH4 )2 SO4 , 150 mM KCl, 2 mM MgSO4 , 0.1% Tween 20 pH 8.8 at 25 °C), 4 mM additional MgSO4 (beyond 2 mM from buffer), 1.4 mM Deoxynucleotide Solution Mix. .. Primers: 1.6 μM FIP/BIP, 0.2 μM FOP/BOP, and 0.4 μM LoopF/LoopB, 1 mg/mL BSA (New England BioLabs, ref B90005), 320 U/mL Bst 3.0, Ambion RNase cocktail (ThermoFisher, Waltham, MA, USA; ref AM2286, 5 U/mL RNase A, 400 U/mL TNase T1), 2 μM SYTO 9 (ThermoFisher, ref S34854), and approximately 660 copies/μL template in Ambion nuclease-free water (ThermoFisher, ref AM9932).

    Article Title: Visual and modular detection of pathogen nucleic acids with enzyme–DNA molecular complexes
    Article Snippet: .. This mixture was incubated with 8 units of Bst 2.0 DNA polymerase (New England Biolabs) in 1× isothermal amplification buffer II, 6 mM MgSO4 , 2.5 mM dNTPs, at 70 °C for 1 h. The amplification product was purified via PCR cleanup spin columns (New England Biolabs) and quantified through gel electrophoresis. ..

    Article Title: Optical Temperature Control Unit and Convolutional Neural Network for Colorimetric Detection of Loop-Mediated Isothermal Amplification on a Lab-On-A-Disc Platform
    Article Snippet: .. LAMP Reaction The LAMP reaction mixture was set based on following reagents: 10X Isothermal Amplification Buffer II (B0374S, New England Biolabs, USA, 1X Buffer components: 20 mM Tris-HCl, 10 mM (NH4 )2 SO4 , 150 mM KCl, 2 mM MgSO4 , and 0.1% Tween® 20. pH 8.8 at 25 °C), 6 mM MgSO4 (8 mM total), dNTP Mix for 1.4 mM each (11 969 064 001, Roche, Basel, Switzerland), 1.6 μM of FIP/BIP primers, 0.2 µM of F3/B3 primers, 0.8 μM of LoopF/LoopB primers, 8U of Bst 3.0 DNA Polymerase (M0374S, New England Biolabs, Ipswich, USA), and 120 µM of HNB (H007-10, Dojindo, Kamimashiki, Japan) in a total volume of 25 µL following the manual instructions. ..

    Article Title: Integrated sample inactivation, amplification, and Cas13-based detection of SARS-CoV-2
    Article Snippet: .. Briefly, the assay was performed in two steps: (1) isothermal amplification via recombinase polymerase amplification (RPA) and (2) Lwa Cas13a-based detection using a single-stranded RNA (ssRNA) fluorescent reporter. .. For RPA, the TwistAmp Basic Kit (TwistDx) was used as previously described (i.e. , with RPA forward and reverse primer concentrations of 400 nM and a magnesium acetate concentration of 14 mM) ( ) with the following modifications: RevertAid reverse transcriptase (Thermo Fisher Scientific) and murine RNase inhibitor (NEB) were added at final concentrations of 4 U/µl each, and synthetic RNAs or viral seedstocks were added at known input concentrations making up 10% of the total reaction volume.

    Purification:

    Article Title: Visual and modular detection of pathogen nucleic acids with enzyme–DNA molecular complexes
    Article Snippet: .. This mixture was incubated with 8 units of Bst 2.0 DNA polymerase (New England Biolabs) in 1× isothermal amplification buffer II, 6 mM MgSO4 , 2.5 mM dNTPs, at 70 °C for 1 h. The amplification product was purified via PCR cleanup spin columns (New England Biolabs) and quantified through gel electrophoresis. ..

    SYBR Green Assay:

    Article Title: The Influence of Reaction Conditions on DNA Multimerization During Isothermal Amplification with Bst exo- DNA Polymerase.
    Article Snippet: .. Methods for isothermal amplification of nucleic acids are gained more attention in the last two decades. .. Methods for isothermal amplification of nucleic acids are gained more attention in the last two decades.

    Incubation:

    Article Title: Visual and modular detection of pathogen nucleic acids with enzyme–DNA molecular complexes
    Article Snippet: .. This mixture was incubated with 8 units of Bst 2.0 DNA polymerase (New England Biolabs) in 1× isothermal amplification buffer II, 6 mM MgSO4 , 2.5 mM dNTPs, at 70 °C for 1 h. The amplification product was purified via PCR cleanup spin columns (New England Biolabs) and quantified through gel electrophoresis. ..

    Polymerase Chain Reaction:

    Article Title: Visual and modular detection of pathogen nucleic acids with enzyme–DNA molecular complexes
    Article Snippet: .. This mixture was incubated with 8 units of Bst 2.0 DNA polymerase (New England Biolabs) in 1× isothermal amplification buffer II, 6 mM MgSO4 , 2.5 mM dNTPs, at 70 °C for 1 h. The amplification product was purified via PCR cleanup spin columns (New England Biolabs) and quantified through gel electrophoresis. ..

    Recombinase Polymerase Amplification:

    Article Title: Integrated sample inactivation, amplification, and Cas13-based detection of SARS-CoV-2
    Article Snippet: .. Briefly, the assay was performed in two steps: (1) isothermal amplification via recombinase polymerase amplification (RPA) and (2) Lwa Cas13a-based detection using a single-stranded RNA (ssRNA) fluorescent reporter. .. For RPA, the TwistAmp Basic Kit (TwistDx) was used as previously described (i.e. , with RPA forward and reverse primer concentrations of 400 nM and a magnesium acetate concentration of 14 mM) ( ) with the following modifications: RevertAid reverse transcriptase (Thermo Fisher Scientific) and murine RNase inhibitor (NEB) were added at final concentrations of 4 U/µl each, and synthetic RNAs or viral seedstocks were added at known input concentrations making up 10% of the total reaction volume.

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  • 96
    New England Biolabs isothermal amplification buffer ii
    Isothermal Amplification Buffer Ii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isothermal amplification buffer ii/product/New England Biolabs
    Average 96 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    isothermal amplification buffer ii - by Bioz Stars, 2020-10
    96/100 stars
      Buy from Supplier

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