dsdna fragmentase buffer  (New England Biolabs)


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  • 99
    Name:
    NEBNext dsDNA Fragmentase Reaction Buffer
    Description:
    NEBNext dsDNA Fragmentase Reaction Buffer 6 0 ml
    Catalog Number:
    B0348S
    Price:
    21
    Size:
    6 0 ml
    Category:
    DNA Analysis
    Score:
    85
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    Structured Review

    New England Biolabs dsdna fragmentase buffer
    NEBNext dsDNA Fragmentase Reaction Buffer
    NEBNext dsDNA Fragmentase Reaction Buffer 6 0 ml
    https://www.bioz.com/result/dsdna fragmentase buffer/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dsdna fragmentase buffer - by Bioz Stars, 2019-10
    99/100 stars

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    Related Articles

    Multiplexing:

    Article Title: Global unleashing of transcription elongation waves in response to genotoxic stress restricts somatic mutation rate
    Article Snippet: Library preparation was performed essentially according to standard Illumina protocols and as previously described with minor modifications. .. DNA-modifying enzymes were purchased from NEB. dsDNA was end repaired, 3′-end adenylated and ligated to barcoded (for multiplexing) Truseq (synthesised by IDT) or NEBNext adapters according to manufacturer’s guidelines. .. Samples were PCR amplified by insuring reduced amplification bias (optimal cycle number was determined by qPCR using the Illumina P5 and P7 primers) and size-selected (200–500 bp) through double selection by Ampure XP Beads as previously described .

    Electrophoresis:

    Article Title: Detection of novel mutations that cause autosomal dominant retinitis pigmentosa in candidate genes by long-range PCR amplification and next-generation sequencing
    Article Snippet: Nevertheless, before the library was prepared and because of the differences in size and GC content of the 20 LR-PCR fragments, we assayed each fragment in a kinetic reaction with NEBNext Fragmentase enzyme (BioLabs Izasa, Barcelona, Spain). .. Nevertheless, before the library was prepared and because of the differences in size and GC content of the 20 LR-PCR fragments, we assayed each fragment in a kinetic reaction with NEBNext Fragmentase enzyme (BioLabs Izasa, Barcelona, Spain).

    Negative Control:

    Article Title: MAGERI: Computational pipeline for molecular-barcoded targeted resequencing
    Article Snippet: Positive control sample was obtained by using a Tru-Q 7 1% Tier reference mutation panel (Horizon Dx, USA; Cat. ID HD734) and by mixing Tru-Q 7 1% Tier reference with negative control sample at 1: 9 ratio. .. Tru-Q 7 mutation panel and negative control DNA was fragmented with dsDNA Fragmentase (NEB, cat. # M0348) and dsDNA Fragmentase buffer (NEB, cat. # B0348) according to manufacturer's protocol. .. DNA concentration was determined by Qubit fluorometry and real-time qPCR with both methods giving comparable results.

    Gas Chromatography:

    Article Title: Detection of novel mutations that cause autosomal dominant retinitis pigmentosa in candidate genes by long-range PCR amplification and next-generation sequencing
    Article Snippet: Moreover, no additional instruments or installations are necessary. .. Nevertheless, before the library was prepared and because of the differences in size and GC content of the 20 LR-PCR fragments, we assayed each fragment in a kinetic reaction with NEBNext Fragmentase enzyme (BioLabs Izasa, Barcelona, Spain). .. Briefly, the Fragmentase reaction was performed in 50 μl at 0.03 μg/μl of DNA and incubated with 3 μl of enzyme.

    Agarose Gel Electrophoresis:

    Article Title: Detection of novel mutations that cause autosomal dominant retinitis pigmentosa in candidate genes by long-range PCR amplification and next-generation sequencing
    Article Snippet: Nevertheless, before the library was prepared and because of the differences in size and GC content of the 20 LR-PCR fragments, we assayed each fragment in a kinetic reaction with NEBNext Fragmentase enzyme (BioLabs Izasa, Barcelona, Spain). .. Nevertheless, before the library was prepared and because of the differences in size and GC content of the 20 LR-PCR fragments, we assayed each fragment in a kinetic reaction with NEBNext Fragmentase enzyme (BioLabs Izasa, Barcelona, Spain).

    Positive Control:

    Article Title: MAGERI: Computational pipeline for molecular-barcoded targeted resequencing
    Article Snippet: Positive control sample was obtained by using a Tru-Q 7 1% Tier reference mutation panel (Horizon Dx, USA; Cat. ID HD734) and by mixing Tru-Q 7 1% Tier reference with negative control sample at 1: 9 ratio. .. Tru-Q 7 mutation panel and negative control DNA was fragmented with dsDNA Fragmentase (NEB, cat. # M0348) and dsDNA Fragmentase buffer (NEB, cat. # B0348) according to manufacturer's protocol.

    Mutagenesis:

    Article Title: MAGERI: Computational pipeline for molecular-barcoded targeted resequencing
    Article Snippet: Positive control sample was obtained by using a Tru-Q 7 1% Tier reference mutation panel (Horizon Dx, USA; Cat. ID HD734) and by mixing Tru-Q 7 1% Tier reference with negative control sample at 1: 9 ratio. .. Tru-Q 7 mutation panel and negative control DNA was fragmented with dsDNA Fragmentase (NEB, cat. # M0348) and dsDNA Fragmentase buffer (NEB, cat. # B0348) according to manufacturer's protocol. .. DNA concentration was determined by Qubit fluorometry and real-time qPCR with both methods giving comparable results.

    Next-Generation Sequencing:

    Article Title: Detection of novel mutations that cause autosomal dominant retinitis pigmentosa in candidate genes by long-range PCR amplification and next-generation sequencing
    Article Snippet: Paragraph title: NEBNext dsDNA fragmentase and NGS library preparation ... Nevertheless, before the library was prepared and because of the differences in size and GC content of the 20 LR-PCR fragments, we assayed each fragment in a kinetic reaction with NEBNext Fragmentase enzyme (BioLabs Izasa, Barcelona, Spain).

    Article Title: Global unleashing of transcription elongation waves in response to genotoxic stress restricts somatic mutation rate
    Article Snippet: DNA-modifying enzymes were purchased from NEB. dsDNA was end repaired, 3′-end adenylated and ligated to barcoded (for multiplexing) Truseq (synthesised by IDT) or NEBNext adapters according to manufacturer’s guidelines. .. Samples were PCR amplified by insuring reduced amplification bias (optimal cycle number was determined by qPCR using the Illumina P5 and P7 primers) and size-selected (200–500 bp) through double selection by Ampure XP Beads as previously described .

    Construct:

    Article Title: MAGERI: Computational pipeline for molecular-barcoded targeted resequencing
    Article Snippet: For determination of analytical sensitivity and selectivity of the method, negative and positive control DNA samples were constructed. .. Tru-Q 7 mutation panel and negative control DNA was fragmented with dsDNA Fragmentase (NEB, cat. # M0348) and dsDNA Fragmentase buffer (NEB, cat. # B0348) according to manufacturer's protocol.

    Real-time Polymerase Chain Reaction:

    Article Title: MAGERI: Computational pipeline for molecular-barcoded targeted resequencing
    Article Snippet: Tru-Q 7 mutation panel and negative control DNA was fragmented with dsDNA Fragmentase (NEB, cat. # M0348) and dsDNA Fragmentase buffer (NEB, cat. # B0348) according to manufacturer's protocol. .. DNA concentration was determined by Qubit fluorometry and real-time qPCR with both methods giving comparable results.

    Sequencing:

    Article Title: Detection of novel mutations that cause autosomal dominant retinitis pigmentosa in candidate genes by long-range PCR amplification and next-generation sequencing
    Article Snippet: This enzymatic method, outlined in , was used to prepare the DNA libraries for sequencing. .. Nevertheless, before the library was prepared and because of the differences in size and GC content of the 20 LR-PCR fragments, we assayed each fragment in a kinetic reaction with NEBNext Fragmentase enzyme (BioLabs Izasa, Barcelona, Spain).

    Article Title: Global unleashing of transcription elongation waves in response to genotoxic stress restricts somatic mutation rate
    Article Snippet: Paragraph title: Illumina sequencing and library generation ... DNA-modifying enzymes were purchased from NEB. dsDNA was end repaired, 3′-end adenylated and ligated to barcoded (for multiplexing) Truseq (synthesised by IDT) or NEBNext adapters according to manufacturer’s guidelines.

    Blocking Assay:

    Article Title: MAGERI: Computational pipeline for molecular-barcoded targeted resequencing
    Article Snippet: Tru-Q 7 mutation panel and negative control DNA was fragmented with dsDNA Fragmentase (NEB, cat. # M0348) and dsDNA Fragmentase buffer (NEB, cat. # B0348) according to manufacturer's protocol. .. Control samples were further tested for mutations in the hotspots of KRAS exon 2, NRAS exon 3, BRAF exon 15 and EGFR exons 18–21 using Insider Mutation Detection Kits (Evrogen Lab Ltd, Moscow, Russian Federation) and TaqMan Mutation Detection Reagents (Thermo Fisher, Waltham, Massachusetts, United States).

    Polymerase Chain Reaction:

    Article Title: MAGERI: Computational pipeline for molecular-barcoded targeted resequencing
    Article Snippet: Tru-Q 7 mutation panel and negative control DNA was fragmented with dsDNA Fragmentase (NEB, cat. # M0348) and dsDNA Fragmentase buffer (NEB, cat. # B0348) according to manufacturer's protocol. .. Control samples were further tested for mutations in the hotspots of KRAS exon 2, NRAS exon 3, BRAF exon 15 and EGFR exons 18–21 using Insider Mutation Detection Kits (Evrogen Lab Ltd, Moscow, Russian Federation) and TaqMan Mutation Detection Reagents (Thermo Fisher, Waltham, Massachusetts, United States).

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  • 99
    New England Biolabs dsdna fragmentase buffer
    Dsdna Fragmentase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dsdna fragmentase buffer/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dsdna fragmentase buffer - by Bioz Stars, 2019-10
    99/100 stars
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