dnase i buffer  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98
    Name:
    DNase I Reaction Buffer
    Description:
    DNase I Reaction Buffer 6 0 ml
    Catalog Number:
    b0303s
    Price:
    23
    Size:
    6 0 ml
    Category:
    Buffers
    Buy from Supplier


    Structured Review

    New England Biolabs dnase i buffer
    DNase I Reaction Buffer
    DNase I Reaction Buffer 6 0 ml
    https://www.bioz.com/result/dnase i buffer/product/New England Biolabs
    Average 98 stars, based on 107 article reviews
    Price from $9.99 to $1999.99
    dnase i buffer - by Bioz Stars, 2020-08
    98/100 stars

    Images

    1) Product Images from "Glucose-Dependent Activation of Bacillus anthracis Toxin Gene Expression and Virulence Requires the Carbon Catabolite Protein CcpA ▿ Toxin Gene Expression and Virulence Requires the Carbon Catabolite Protein CcpA ▿ †"

    Article Title: Glucose-Dependent Activation of Bacillus anthracis Toxin Gene Expression and Virulence Requires the Carbon Catabolite Protein CcpA ▿ Toxin Gene Expression and Virulence Requires the Carbon Catabolite Protein CcpA ▿ †

    Journal: Journal of Bacteriology

    doi: 10.1128/JB.01656-09

    DNase I footprinting analysis of CcpA binding to the atxA (A and B) and BAS3893 (C and D) promoters. Fragments labeled with γ- 32 P at the 5′ end (coding strand) (A and C) or at the 3′ end (noncoding strand) (B and D) were incubated
    Figure Legend Snippet: DNase I footprinting analysis of CcpA binding to the atxA (A and B) and BAS3893 (C and D) promoters. Fragments labeled with γ- 32 P at the 5′ end (coding strand) (A and C) or at the 3′ end (noncoding strand) (B and D) were incubated

    Techniques Used: Footprinting, Binding Assay, Labeling, Incubation

    2) Product Images from "Enterococcus faecalis Virulence Regulator FsrA Binding to Target Promoters ▿"

    Article Title: Enterococcus faecalis Virulence Regulator FsrA Binding to Target Promoters ▿

    Journal: Journal of Bacteriology

    doi: 10.1128/JB.01522-10

    DNase I footprinting analysis of the promoter-regulatory regions of fsrB (A) and gelE (B). The labeled DNA fragments were obtained as described in Materials and Methods. The fragments were incubated with different amounts of FsrA-His6 before DNase I treatment.
    Figure Legend Snippet: DNase I footprinting analysis of the promoter-regulatory regions of fsrB (A) and gelE (B). The labeled DNA fragments were obtained as described in Materials and Methods. The fragments were incubated with different amounts of FsrA-His6 before DNase I treatment.

    Techniques Used: Footprinting, Labeling, Incubation

    3) Product Images from "An in situ atlas of mitochondrial DNA in mammalian tissues reveals high content in stem/progenitor cells"

    Article Title: An in situ atlas of mitochondrial DNA in mammalian tissues reveals high content in stem/progenitor cells

    Journal: bioRxiv

    doi: 10.1101/2019.12.19.876144

    In situ assay specificity verified by DNase pretreatment. MT-CO1 sense DNA in situ hybridization assay on FFPE prostate tissues without RNase A ( A ), with RNase A ( B ), without DNase I ( C ), and with DNase I ( D ) pretreatments. Original magnification x40.
    Figure Legend Snippet: In situ assay specificity verified by DNase pretreatment. MT-CO1 sense DNA in situ hybridization assay on FFPE prostate tissues without RNase A ( A ), with RNase A ( B ), without DNase I ( C ), and with DNase I ( D ) pretreatments. Original magnification x40.

    Techniques Used: In Situ, DNA In Situ Hybridization, Formalin-fixed Paraffin-Embedded

    4) Product Images from "Dimerization and DNA-dependent aggregation of the Escherichia coli nucleoid protein and chaperone CbpA"

    Article Title: Dimerization and DNA-dependent aggregation of the Escherichia coli nucleoid protein and chaperone CbpA

    Journal: Molecular Microbiology

    doi: 10.1111/j.1365-2958.2010.07292.x

    CbpA protects plasmid DNA from degradation by nucleases. A. The panel shows naked plasmid and complexes with CbpA run on a 1% agarose gel. Plasmid (30 ng) was pre-incubated with 0, 0.5, 1.0, or 2.0 µM CbpA. Note that only every other lane has been loaded on the gel. B. The panel shows plasmid run on a 1% agarose gel. Plasmids (77 ng) were treated with different combinations of DNase I, BSA (1, 2 or 3 µM) and CbpA (1, 2 or 3 µM).
    Figure Legend Snippet: CbpA protects plasmid DNA from degradation by nucleases. A. The panel shows naked plasmid and complexes with CbpA run on a 1% agarose gel. Plasmid (30 ng) was pre-incubated with 0, 0.5, 1.0, or 2.0 µM CbpA. Note that only every other lane has been loaded on the gel. B. The panel shows plasmid run on a 1% agarose gel. Plasmids (77 ng) were treated with different combinations of DNase I, BSA (1, 2 or 3 µM) and CbpA (1, 2 or 3 µM).

    Techniques Used: Plasmid Preparation, Agarose Gel Electrophoresis, Incubation

    5) Product Images from "A Thermonuclease of Neisseria gonorrhoeae Enhances Bacterial Escape From Killing by Neutrophil Extracellular Traps"

    Article Title: A Thermonuclease of Neisseria gonorrhoeae Enhances Bacterial Escape From Killing by Neutrophil Extracellular Traps

    Journal: The Journal of Infectious Diseases

    doi: 10.1093/infdis/jiv031

    Nuc cleaves human neutrophil and neutrophil extracellular trap DNA. A , Human neutrophil DNA was incubated with increasing amounts of Nuc or DNAse I and separated on ethidium bromide–stained agarose gels. B , Phorbol 12-myristate 13-acetate–stimulated
    Figure Legend Snippet: Nuc cleaves human neutrophil and neutrophil extracellular trap DNA. A , Human neutrophil DNA was incubated with increasing amounts of Nuc or DNAse I and separated on ethidium bromide–stained agarose gels. B , Phorbol 12-myristate 13-acetate–stimulated

    Techniques Used: Incubation, Staining

    Related Articles

    Synthesized:

    Article Title: Viral miRNA adaptor differentially recruits miRNAs to target mRNAs through alternative base-pairing
    Article Snippet: .. RNA was suspended in 85 µl of water, 10 µl of 10X DNAse reaction buffer (New England Biolabs), and 10 units (5 µl) of RNAse-free DNAse I (New England Biolabs) and incubated at 37°C for 1 hr. cDNA was synthesized in 40 µl reactions from 1.8 µg of DNase I-treated total RNA using the High-Capacity cDNA Reverse Transcription Kit with MultiScribe Reverse Transcriptase and random primers (Applied Biosystems). .. Subsequently, real-time PCR was performed in 8 µl reactions using primers (see ) at 0.5 µM, cDNA diluted at a ratio of 1:5 (except MGA for which the cDNA was used undiluted, or for β-actin for which the cDNA was diluted 1:5,000, or for ribosomal 18 s for which the cDNA was diluted 1:50,000) and KAPA SYBR green (KAPA Biosystems) in a Roche 480 Light Cycler (95°C for 5 min, one cycle; 95°C for 10 s, 60°C for 10 s and 72°C for 10 s, 55 cycles followed by a melting curve of the reaction product from 65°C to 97°C with a ramp rate of 0.11 °C s−1 ). qPCR primers were designed using Primer3Plus ( https://primer3plus.com/cgi-bin/dev/primer3plus.cgi ) and tested using cDNA prepared in 20 µl reactions from 900 ng of DNase I-treated total RNA and diluted at ratios1:1–1:5000.

    Labeling:

    Article Title: Enterococcus faecalis Virulence Regulator FsrA Binding to Target Promoters ▿
    Article Snippet: .. The labeled probes (20,000 cpm) were incubated in the presence of FsrA-His6 or its storage buffer at the concentrations indicated in the legend to Fig. 3 in a l5-μl reaction mixture containing 1× DNase I buffer (New England BioLabs). .. Reaction mixtures were incubated for 3 min at room temperature before the addition of DNase I and further incubation for 1 min.

    Article Title: Glucose-Dependent Activation of Bacillus anthracis Toxin Gene Expression and Virulence Requires the Carbon Catabolite Protein CcpA ▿ Toxin Gene Expression and Virulence Requires the Carbon Catabolite Protein CcpA ▿ †
    Article Snippet: .. The labeled probes (20,000 cpm) were incubated in the presence of HPr (5 μM) and CcpA at the concentrations indicated in Fig. 6 and 7 and their legends in a 15-μl reaction mixture containing 1× DNase I buffer (New England Biolabs). .. The reaction mixtures were incubated for 4 min at room temperature before the addition of DNase I and further incubation for 1 min. Stop solution (6 μl; 0.1 M sodium EDTA, 0.5% SDS, 0.4 mg/ml sheared salmon sperm DNA) was added, and DNA precipitation from the reaction mixtures was accomplished with 500 μl of ethanol in an ethanol-dry ice bath.

    Purification:

    Article Title: Dimerization and DNA-dependent aggregation of the Escherichia coli nucleoid protein and chaperone CbpA
    Article Snippet: .. DNase I protection assays Purified plasmid DNA (77 ng) was pre-incubated with CbpA or BSA, at 37°C, in DNase I reaction buffer (NEB) in a final volume of 10 µl. ..

    Concentration Assay:

    Article Title: Packaging of Dinoroseobacter shibae DNA into Gene Transfer Agent Particles Is Not Random
    Article Snippet: .. In this case, the pellets were resuspended in TE buffer with gentle shaking at room temperature for 2 h. Free nucleic acids were removed by treatment with DNase I (0.01 U μl−1 ) and RNase A (0.08 U μl−1 ) at 37 °C in 1× DNase buffer (New England Biolabs) for 1 h. The sample was heated at 75 °C for 15 min after addition of EDTA to a final concentration of 5 mM. .. Nucleic acids were purified by phenol: chloroform: isoamyl alcohol (25:24:1) extraction and ethanol precipitation.

    Incubation:

    Article Title: Enterococcus faecalis Virulence Regulator FsrA Binding to Target Promoters ▿
    Article Snippet: .. The labeled probes (20,000 cpm) were incubated in the presence of FsrA-His6 or its storage buffer at the concentrations indicated in the legend to Fig. 3 in a l5-μl reaction mixture containing 1× DNase I buffer (New England BioLabs). .. Reaction mixtures were incubated for 3 min at room temperature before the addition of DNase I and further incubation for 1 min.

    Article Title: A Thermonuclease of Neisseria gonorrhoeae Enhances Bacterial Escape From Killing by Neutrophil Extracellular Traps
    Article Snippet: .. Neutrophil genomic DNA (3 μg) was incubated for 30 minutes at 37°C in molecular biology–grade water (Thermo Scientific) and DNAse I reaction buffer alone, with 0.5 U of DNAse I (recombinant bovine pancreatic DNAse I ; New England Biolabs), or with recombinant Nuc that was boiled for 10 minutes or left unboiled. .. Nuc was expressed in Escherichia coli and purified as described by Steichen et al [ ].

    Article Title: Glucose-Dependent Activation of Bacillus anthracis Toxin Gene Expression and Virulence Requires the Carbon Catabolite Protein CcpA ▿ Toxin Gene Expression and Virulence Requires the Carbon Catabolite Protein CcpA ▿ †
    Article Snippet: .. The labeled probes (20,000 cpm) were incubated in the presence of HPr (5 μM) and CcpA at the concentrations indicated in Fig. 6 and 7 and their legends in a 15-μl reaction mixture containing 1× DNase I buffer (New England Biolabs). .. The reaction mixtures were incubated for 4 min at room temperature before the addition of DNase I and further incubation for 1 min. Stop solution (6 μl; 0.1 M sodium EDTA, 0.5% SDS, 0.4 mg/ml sheared salmon sperm DNA) was added, and DNA precipitation from the reaction mixtures was accomplished with 500 μl of ethanol in an ethanol-dry ice bath.

    Article Title: Viral miRNA adaptor differentially recruits miRNAs to target mRNAs through alternative base-pairing
    Article Snippet: .. RNA was suspended in 85 µl of water, 10 µl of 10X DNAse reaction buffer (New England Biolabs), and 10 units (5 µl) of RNAse-free DNAse I (New England Biolabs) and incubated at 37°C for 1 hr. cDNA was synthesized in 40 µl reactions from 1.8 µg of DNase I-treated total RNA using the High-Capacity cDNA Reverse Transcription Kit with MultiScribe Reverse Transcriptase and random primers (Applied Biosystems). .. Subsequently, real-time PCR was performed in 8 µl reactions using primers (see ) at 0.5 µM, cDNA diluted at a ratio of 1:5 (except MGA for which the cDNA was used undiluted, or for β-actin for which the cDNA was diluted 1:5,000, or for ribosomal 18 s for which the cDNA was diluted 1:50,000) and KAPA SYBR green (KAPA Biosystems) in a Roche 480 Light Cycler (95°C for 5 min, one cycle; 95°C for 10 s, 60°C for 10 s and 72°C for 10 s, 55 cycles followed by a melting curve of the reaction product from 65°C to 97°C with a ramp rate of 0.11 °C s−1 ). qPCR primers were designed using Primer3Plus ( https://primer3plus.com/cgi-bin/dev/primer3plus.cgi ) and tested using cDNA prepared in 20 µl reactions from 900 ng of DNase I-treated total RNA and diluted at ratios1:1–1:5000.

    Recombinant:

    Article Title: A Thermonuclease of Neisseria gonorrhoeae Enhances Bacterial Escape From Killing by Neutrophil Extracellular Traps
    Article Snippet: .. Neutrophil genomic DNA (3 μg) was incubated for 30 minutes at 37°C in molecular biology–grade water (Thermo Scientific) and DNAse I reaction buffer alone, with 0.5 U of DNAse I (recombinant bovine pancreatic DNAse I ; New England Biolabs), or with recombinant Nuc that was boiled for 10 minutes or left unboiled. .. Nuc was expressed in Escherichia coli and purified as described by Steichen et al [ ].

    Plasmid Preparation:

    Article Title: Dimerization and DNA-dependent aggregation of the Escherichia coli nucleoid protein and chaperone CbpA
    Article Snippet: .. DNase I protection assays Purified plasmid DNA (77 ng) was pre-incubated with CbpA or BSA, at 37°C, in DNase I reaction buffer (NEB) in a final volume of 10 µl. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98
    New England Biolabs dnase i buffer
    <t>DNase</t> I footprinting analysis of CcpA binding to the atxA (A and B) and BAS3893 (C and D) promoters. Fragments labeled with γ- 32 P at the 5′ end (coding strand) (A and C) or at the 3′ end (noncoding strand) (B and D) were incubated
    Dnase I Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase i buffer/product/New England Biolabs
    Average 98 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    dnase i buffer - by Bioz Stars, 2020-08
    98/100 stars
      Buy from Supplier

    Image Search Results


    DNase I footprinting analysis of CcpA binding to the atxA (A and B) and BAS3893 (C and D) promoters. Fragments labeled with γ- 32 P at the 5′ end (coding strand) (A and C) or at the 3′ end (noncoding strand) (B and D) were incubated

    Journal: Journal of Bacteriology

    Article Title: Glucose-Dependent Activation of Bacillus anthracis Toxin Gene Expression and Virulence Requires the Carbon Catabolite Protein CcpA ▿ Toxin Gene Expression and Virulence Requires the Carbon Catabolite Protein CcpA ▿ †

    doi: 10.1128/JB.01656-09

    Figure Lengend Snippet: DNase I footprinting analysis of CcpA binding to the atxA (A and B) and BAS3893 (C and D) promoters. Fragments labeled with γ- 32 P at the 5′ end (coding strand) (A and C) or at the 3′ end (noncoding strand) (B and D) were incubated

    Article Snippet: The labeled probes (20,000 cpm) were incubated in the presence of HPr (5 μM) and CcpA at the concentrations indicated in Fig. 6 and 7 and their legends in a 15-μl reaction mixture containing 1× DNase I buffer (New England Biolabs).

    Techniques: Footprinting, Binding Assay, Labeling, Incubation

    DNase I footprinting analysis of the promoter-regulatory regions of fsrB (A) and gelE (B). The labeled DNA fragments were obtained as described in Materials and Methods. The fragments were incubated with different amounts of FsrA-His6 before DNase I treatment.

    Journal: Journal of Bacteriology

    Article Title: Enterococcus faecalis Virulence Regulator FsrA Binding to Target Promoters ▿

    doi: 10.1128/JB.01522-10

    Figure Lengend Snippet: DNase I footprinting analysis of the promoter-regulatory regions of fsrB (A) and gelE (B). The labeled DNA fragments were obtained as described in Materials and Methods. The fragments were incubated with different amounts of FsrA-His6 before DNase I treatment.

    Article Snippet: The labeled probes (20,000 cpm) were incubated in the presence of FsrA-His6 or its storage buffer at the concentrations indicated in the legend to Fig. 3 in a l5-μl reaction mixture containing 1× DNase I buffer (New England BioLabs).

    Techniques: Footprinting, Labeling, Incubation

    In situ assay specificity verified by DNase pretreatment. MT-CO1 sense DNA in situ hybridization assay on FFPE prostate tissues without RNase A ( A ), with RNase A ( B ), without DNase I ( C ), and with DNase I ( D ) pretreatments. Original magnification x40.

    Journal: bioRxiv

    Article Title: An in situ atlas of mitochondrial DNA in mammalian tissues reveals high content in stem/progenitor cells

    doi: 10.1101/2019.12.19.876144

    Figure Lengend Snippet: In situ assay specificity verified by DNase pretreatment. MT-CO1 sense DNA in situ hybridization assay on FFPE prostate tissues without RNase A ( A ), with RNase A ( B ), without DNase I ( C ), and with DNase I ( D ) pretreatments. Original magnification x40.

    Article Snippet: For DNase pretreatment, after steaming in Pretreatment II, the slides were treated with 100 μL DNase reaction buffer containing 10 μL DNase I Reaction Buffer (10X), 1 μL (2 units) DNAse I (M0303S, New England BioLabs, Ipswich, MA), and 89 μL nuclease-free H2 O.

    Techniques: In Situ, DNA In Situ Hybridization, Formalin-fixed Paraffin-Embedded

    CbpA protects plasmid DNA from degradation by nucleases. A. The panel shows naked plasmid and complexes with CbpA run on a 1% agarose gel. Plasmid (30 ng) was pre-incubated with 0, 0.5, 1.0, or 2.0 µM CbpA. Note that only every other lane has been loaded on the gel. B. The panel shows plasmid run on a 1% agarose gel. Plasmids (77 ng) were treated with different combinations of DNase I, BSA (1, 2 or 3 µM) and CbpA (1, 2 or 3 µM).

    Journal: Molecular Microbiology

    Article Title: Dimerization and DNA-dependent aggregation of the Escherichia coli nucleoid protein and chaperone CbpA

    doi: 10.1111/j.1365-2958.2010.07292.x

    Figure Lengend Snippet: CbpA protects plasmid DNA from degradation by nucleases. A. The panel shows naked plasmid and complexes with CbpA run on a 1% agarose gel. Plasmid (30 ng) was pre-incubated with 0, 0.5, 1.0, or 2.0 µM CbpA. Note that only every other lane has been loaded on the gel. B. The panel shows plasmid run on a 1% agarose gel. Plasmids (77 ng) were treated with different combinations of DNase I, BSA (1, 2 or 3 µM) and CbpA (1, 2 or 3 µM).

    Article Snippet: DNase I protection assays Purified plasmid DNA (77 ng) was pre-incubated with CbpA or BSA, at 37°C, in DNase I reaction buffer (NEB) in a final volume of 10 µl.

    Techniques: Plasmid Preparation, Agarose Gel Electrophoresis, Incubation