dna ligase buffer  (New England Biolabs)


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    Name:
    Taq DNA Ligase Reaction Buffer
    Description:
    Taq DNA Ligase Reaction Buffer 6 0 ml
    Catalog Number:
    b0208s
    Price:
    24
    Size:
    6 0 ml
    Category:
    Buffers
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    Structured Review

    New England Biolabs dna ligase buffer
    Taq DNA Ligase Reaction Buffer
    Taq DNA Ligase Reaction Buffer 6 0 ml
    https://www.bioz.com/result/dna ligase buffer/product/New England Biolabs
    Average 94 stars, based on 138 article reviews
    Price from $9.99 to $1999.99
    dna ligase buffer - by Bioz Stars, 2020-09
    94/100 stars

    Images

    1) Product Images from "A Complete Mitochondrial Genome Sequence from a Mesolithic Wild Aurochs (Bos primigenius)"

    Article Title: A Complete Mitochondrial Genome Sequence from a Mesolithic Wild Aurochs (Bos primigenius)

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0009255

    The identity and distribution of DNA nucleotide mismatches in individual Illumina GA reads compared to the CPC98 consensus mtDNA genome. (A) The number and proportion of each nucleotide called in the Illumina GA reads (vertical column) compared to the consensus mtDNA sequence (horizontal column) is presented. (B) Mean percentage of discordant nucleotides for each position across all individual Illumina GA sequence reads.
    Figure Legend Snippet: The identity and distribution of DNA nucleotide mismatches in individual Illumina GA reads compared to the CPC98 consensus mtDNA genome. (A) The number and proportion of each nucleotide called in the Illumina GA reads (vertical column) compared to the consensus mtDNA sequence (horizontal column) is presented. (B) Mean percentage of discordant nucleotides for each position across all individual Illumina GA sequence reads.

    Techniques Used: Sequencing

    2) Product Images from "Genomic Characterization of Sixteen Yersinia enterocolitica-Infecting Podoviruses of Pig Origin"

    Article Title: Genomic Characterization of Sixteen Yersinia enterocolitica-Infecting Podoviruses of Pig Origin

    Journal: Viruses

    doi: 10.3390/v10040174

    The genomic map of fPS-7. The predicted genes are arranged in the direction of transcription shown by different colored arrows. Genes involved in nucleotide metabolism, DNA replication, recombination or repair are shown in green. Genes involved in morphogenesis and virion structures are depicted in brown. Genes involved in DNA packaging and lysis, are shown in blue and red, respectively. Genes coding for hypothetical proteins or conserved phage proteins of unknown function are shown in light grey. Homing endonucleases are shown in yellow. Direct terminal repeats (DTRs) are shown in black. On top of the genome, the host RNA polymerase (RNAP)-dependent promoters are shown with red double-arrows labelled with −35 and −10, and the phage RNAP-dependent promoters with black arrows labelled from P1 to P12. Terminators are shown along the genome as purple triangles and labelled from T1 to T4. The genetic map was created using the Geneious software.
    Figure Legend Snippet: The genomic map of fPS-7. The predicted genes are arranged in the direction of transcription shown by different colored arrows. Genes involved in nucleotide metabolism, DNA replication, recombination or repair are shown in green. Genes involved in morphogenesis and virion structures are depicted in brown. Genes involved in DNA packaging and lysis, are shown in blue and red, respectively. Genes coding for hypothetical proteins or conserved phage proteins of unknown function are shown in light grey. Homing endonucleases are shown in yellow. Direct terminal repeats (DTRs) are shown in black. On top of the genome, the host RNA polymerase (RNAP)-dependent promoters are shown with red double-arrows labelled with −35 and −10, and the phage RNAP-dependent promoters with black arrows labelled from P1 to P12. Terminators are shown along the genome as purple triangles and labelled from T1 to T4. The genetic map was created using the Geneious software.

    Techniques Used: Lysis, Software

    3) Product Images from "Genomic Characterization of Sixteen Yersinia enterocolitica-Infecting Podoviruses of Pig Origin"

    Article Title: Genomic Characterization of Sixteen Yersinia enterocolitica-Infecting Podoviruses of Pig Origin

    Journal: Viruses

    doi: 10.3390/v10040174

    The genomic map of fPS-7. The predicted genes are arranged in the direction of transcription shown by different colored arrows. Genes involved in nucleotide metabolism, DNA replication, recombination or repair are shown in green. Genes involved in morphogenesis and virion structures are depicted in brown. Genes involved in DNA packaging and lysis, are shown in blue and red, respectively. Genes coding for hypothetical proteins or conserved phage proteins of unknown function are shown in light grey. Homing endonucleases are shown in yellow. Direct terminal repeats (DTRs) are shown in black. On top of the genome, the host RNA polymerase (RNAP)-dependent promoters are shown with red double-arrows labelled with −35 and −10, and the phage RNAP-dependent promoters with black arrows labelled from P1 to P12. Terminators are shown along the genome as purple triangles and labelled from T1 to T4. The genetic map was created using the Geneious software.
    Figure Legend Snippet: The genomic map of fPS-7. The predicted genes are arranged in the direction of transcription shown by different colored arrows. Genes involved in nucleotide metabolism, DNA replication, recombination or repair are shown in green. Genes involved in morphogenesis and virion structures are depicted in brown. Genes involved in DNA packaging and lysis, are shown in blue and red, respectively. Genes coding for hypothetical proteins or conserved phage proteins of unknown function are shown in light grey. Homing endonucleases are shown in yellow. Direct terminal repeats (DTRs) are shown in black. On top of the genome, the host RNA polymerase (RNAP)-dependent promoters are shown with red double-arrows labelled with −35 and −10, and the phage RNAP-dependent promoters with black arrows labelled from P1 to P12. Terminators are shown along the genome as purple triangles and labelled from T1 to T4. The genetic map was created using the Geneious software.

    Techniques Used: Lysis, Software

    Related Articles

    Incubation:

    Article Title: A Complete Mitochondrial Genome Sequence from a Mesolithic Wild Aurochs (Bos primigenius)
    Article Snippet: .. Illumina Genome Analyzer adaptor ligation Ligation reactions (in 50 µl volumes) involved incubation of 19 µl of phosphorylated blunt-ended aurochs DNA extracts, with a 3′-dATP overhang, with 1× DNA ligase buffer (NEB), 1 µl of the proprietary Illumina GA single-read genomic adaptors (Illumina, catalogue no. FC-102-1003) and 10 units T4 DNA ligase (Invitrogen). .. Extracts were incubated at room temperature for 15 minutes, purified using QIAquick MinElute Kit (Qiagen) and eluted in 19 µl of elution buffer according to manufacturer's instructions.

    Article Title: Processing of eukaryotic Okazaki fragments by redundant nucleases can be uncoupled from ongoing DNA replication in vivo
    Article Snippet: .. For in vitro ligation experiments, samples were incubated in 1× DNA ligase buffer (NEB). .. 2 μl of T4 DNA ligase (NEB) was added and samples were incubated for 90 min at room temperature before phenol extraction and end-labeling.

    Article Title: Assembly, purification and crystallization of an active HIV-1 reverse transcriptase initiation complex
    Article Snippet: .. DNA oligonucleotides (Table ; HHMI-Keck Biotechnology Center, Yale University) encoding a T7 RNA polymerase promoter, human tRNALys3 , and two HH ribozymes , were denatured for 2 min at 95°C, then incubated for 1 h at room temperature in a 50 µl reaction containing 4 µM each DNA oligonucleotide, 40 U/µl T4 DNA ligase (New England Biolabs) and 1× DNA ligase buffer (NEB; 50 mM Tris–HCl, pH 7.5, 10 mM MgCl2 , 10 mM DTT, 1 mM ATP, 25 µg/ml BSA). .. The DNA was digested with Eco RI and Bam HI (New England Biolabs) and ligated into the high-copy plasmid pUC19 that had been digested with the same enzymes.

    In Vitro:

    Article Title: Processing of eukaryotic Okazaki fragments by redundant nucleases can be uncoupled from ongoing DNA replication in vivo
    Article Snippet: .. For in vitro ligation experiments, samples were incubated in 1× DNA ligase buffer (NEB). .. 2 μl of T4 DNA ligase (NEB) was added and samples were incubated for 90 min at room temperature before phenol extraction and end-labeling.

    Ligation:

    Article Title: A Complete Mitochondrial Genome Sequence from a Mesolithic Wild Aurochs (Bos primigenius)
    Article Snippet: .. Illumina Genome Analyzer adaptor ligation Ligation reactions (in 50 µl volumes) involved incubation of 19 µl of phosphorylated blunt-ended aurochs DNA extracts, with a 3′-dATP overhang, with 1× DNA ligase buffer (NEB), 1 µl of the proprietary Illumina GA single-read genomic adaptors (Illumina, catalogue no. FC-102-1003) and 10 units T4 DNA ligase (Invitrogen). .. Extracts were incubated at room temperature for 15 minutes, purified using QIAquick MinElute Kit (Qiagen) and eluted in 19 µl of elution buffer according to manufacturer's instructions.

    Article Title: Genomic Characterization of Sixteen Yersinia enterocolitica-Infecting Podoviruses of Pig Origin
    Article Snippet: .. The kinase was then inactivated at 75 °C for 15 min. Ligation of the phosphorylated DNA to a 500 bp fragment of the fliC gene of Y. enterocolitica O:3 was performed in a 30 µL reaction mixture containing ~400 ng of the phosphorylated DNA, ~40 ng of fliC gene fragment, 2 µL of 50% ( w / v ) PEG 4000 (Thermo Scientific), 3 µL of 10 × T4 µL DNA Ligase buffer and 1 µL T4 DNA ligase (New England BioLabs). .. The ligation mixture was then used as a template in PCR that was carried out in a total volume of 50 µL containing 5 µL of 10× buffer for DyNAzyme DNA polymerase (Thermo Scientific), 200 µM dNTPs (Bioline, London, UK), 1 µL of 1:10 diluted fliC -ligated DNA mixture and 2U of DyNAzyme II DNA polymerase (Thermo Scientific, Vilnius, Lithuania).

    Article Title: Processing of eukaryotic Okazaki fragments by redundant nucleases can be uncoupled from ongoing DNA replication in vivo
    Article Snippet: .. For in vitro ligation experiments, samples were incubated in 1× DNA ligase buffer (NEB). .. 2 μl of T4 DNA ligase (NEB) was added and samples were incubated for 90 min at room temperature before phenol extraction and end-labeling.

    Article Title: Genomic Characterization of Sixteen Yersinia enterocolitica-Infecting Podoviruses of Pig Origin
    Article Snippet: .. The kinase was then inactivated at 75 °C for 15 min. Ligation of the phosphorylated DNA to a 500 bp fragment of the fliC gene of Y. enterocolitica O:3 was performed in a 30 µL reaction mixture containing ~400 ng of the phosphorylated DNA, ~40 ng of fliC gene fragment, 2 µL of 50% (w /v ) PEG 4000 (Thermo Scientific), 3 µL of 10 × T4 µL DNA Ligase buffer and 1 µL T4 DNA ligase (New England BioLabs). .. The ligation mixture was then used as a template in PCR that was carried out in a total volume of 50 µL containing 5 µL of 10× buffer for DyNAzyme DNA polymerase (Thermo Scientific), 200 µM dNTPs (Bioline, London, UK), 1 µL of 1:10 diluted fliC -ligated DNA mixture and 2U of DyNAzyme II DNA polymerase (Thermo Scientific, Vilnius, Lithuania).

    Article Title: Multiplex ligation reaction based on probe melting curve analysis: a pragmatic approach for the identification of 30 common Salmonella serovars
    Article Snippet: .. Each 10 μL reaction mix contained 1.5 μL ligation probe mix (1–4 fmol of each specific ligation oligonucleotides), 1U Taq DNA ligase and 1 μL DNA ligase buffer (New England Biolabs, Bejing, China), 1.5 μL diethylpyrocarbonate (DEPC) water and 5 μL genomic DNA. .. Reaction conditions included an initial DNA denaturation step at 98 °C for 5 min containing only genomic DNA and ligation probe mix, a 75 °C hold to add the remainder of reaction mixture (3.5 μL), incubation step at 60 °C for 80 min, 95 °C for 5 min, and finally cooled to 4 °C.

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  • 94
    New England Biolabs dna ligase buffer
    The identity and distribution of <t>DNA</t> nucleotide mismatches in individual <t>Illumina</t> GA reads compared to the CPC98 consensus mtDNA genome. (A) The number and proportion of each nucleotide called in the Illumina GA reads (vertical column) compared to the consensus mtDNA sequence (horizontal column) is presented. (B) Mean percentage of discordant nucleotides for each position across all individual Illumina GA sequence reads.
    Dna Ligase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna ligase buffer/product/New England Biolabs
    Average 94 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    dna ligase buffer - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    The identity and distribution of DNA nucleotide mismatches in individual Illumina GA reads compared to the CPC98 consensus mtDNA genome. (A) The number and proportion of each nucleotide called in the Illumina GA reads (vertical column) compared to the consensus mtDNA sequence (horizontal column) is presented. (B) Mean percentage of discordant nucleotides for each position across all individual Illumina GA sequence reads.

    Journal: PLoS ONE

    Article Title: A Complete Mitochondrial Genome Sequence from a Mesolithic Wild Aurochs (Bos primigenius)

    doi: 10.1371/journal.pone.0009255

    Figure Lengend Snippet: The identity and distribution of DNA nucleotide mismatches in individual Illumina GA reads compared to the CPC98 consensus mtDNA genome. (A) The number and proportion of each nucleotide called in the Illumina GA reads (vertical column) compared to the consensus mtDNA sequence (horizontal column) is presented. (B) Mean percentage of discordant nucleotides for each position across all individual Illumina GA sequence reads.

    Article Snippet: Illumina Genome Analyzer adaptor ligation Ligation reactions (in 50 µl volumes) involved incubation of 19 µl of phosphorylated blunt-ended aurochs DNA extracts, with a 3′-dATP overhang, with 1× DNA ligase buffer (NEB), 1 µl of the proprietary Illumina GA single-read genomic adaptors (Illumina, catalogue no. FC-102-1003) and 10 units T4 DNA ligase (Invitrogen).

    Techniques: Sequencing

    The genomic map of fPS-7. The predicted genes are arranged in the direction of transcription shown by different colored arrows. Genes involved in nucleotide metabolism, DNA replication, recombination or repair are shown in green. Genes involved in morphogenesis and virion structures are depicted in brown. Genes involved in DNA packaging and lysis, are shown in blue and red, respectively. Genes coding for hypothetical proteins or conserved phage proteins of unknown function are shown in light grey. Homing endonucleases are shown in yellow. Direct terminal repeats (DTRs) are shown in black. On top of the genome, the host RNA polymerase (RNAP)-dependent promoters are shown with red double-arrows labelled with −35 and −10, and the phage RNAP-dependent promoters with black arrows labelled from P1 to P12. Terminators are shown along the genome as purple triangles and labelled from T1 to T4. The genetic map was created using the Geneious software.

    Journal: Viruses

    Article Title: Genomic Characterization of Sixteen Yersinia enterocolitica-Infecting Podoviruses of Pig Origin

    doi: 10.3390/v10040174

    Figure Lengend Snippet: The genomic map of fPS-7. The predicted genes are arranged in the direction of transcription shown by different colored arrows. Genes involved in nucleotide metabolism, DNA replication, recombination or repair are shown in green. Genes involved in morphogenesis and virion structures are depicted in brown. Genes involved in DNA packaging and lysis, are shown in blue and red, respectively. Genes coding for hypothetical proteins or conserved phage proteins of unknown function are shown in light grey. Homing endonucleases are shown in yellow. Direct terminal repeats (DTRs) are shown in black. On top of the genome, the host RNA polymerase (RNAP)-dependent promoters are shown with red double-arrows labelled with −35 and −10, and the phage RNAP-dependent promoters with black arrows labelled from P1 to P12. Terminators are shown along the genome as purple triangles and labelled from T1 to T4. The genetic map was created using the Geneious software.

    Article Snippet: The kinase was then inactivated at 75 °C for 15 min. Ligation of the phosphorylated DNA to a 500 bp fragment of the fliC gene of Y. enterocolitica O:3 was performed in a 30 µL reaction mixture containing ~400 ng of the phosphorylated DNA, ~40 ng of fliC gene fragment, 2 µL of 50% (w /v ) PEG 4000 (Thermo Scientific), 3 µL of 10 × T4 µL DNA Ligase buffer and 1 µL T4 DNA ligase (New England BioLabs).

    Techniques: Lysis, Software

    The genomic map of fPS-7. The predicted genes are arranged in the direction of transcription shown by different colored arrows. Genes involved in nucleotide metabolism, DNA replication, recombination or repair are shown in green. Genes involved in morphogenesis and virion structures are depicted in brown. Genes involved in DNA packaging and lysis, are shown in blue and red, respectively. Genes coding for hypothetical proteins or conserved phage proteins of unknown function are shown in light grey. Homing endonucleases are shown in yellow. Direct terminal repeats (DTRs) are shown in black. On top of the genome, the host RNA polymerase (RNAP)-dependent promoters are shown with red double-arrows labelled with −35 and −10, and the phage RNAP-dependent promoters with black arrows labelled from P1 to P12. Terminators are shown along the genome as purple triangles and labelled from T1 to T4. The genetic map was created using the Geneious software.

    Journal: Viruses

    Article Title: Genomic Characterization of Sixteen Yersinia enterocolitica-Infecting Podoviruses of Pig Origin

    doi: 10.3390/v10040174

    Figure Lengend Snippet: The genomic map of fPS-7. The predicted genes are arranged in the direction of transcription shown by different colored arrows. Genes involved in nucleotide metabolism, DNA replication, recombination or repair are shown in green. Genes involved in morphogenesis and virion structures are depicted in brown. Genes involved in DNA packaging and lysis, are shown in blue and red, respectively. Genes coding for hypothetical proteins or conserved phage proteins of unknown function are shown in light grey. Homing endonucleases are shown in yellow. Direct terminal repeats (DTRs) are shown in black. On top of the genome, the host RNA polymerase (RNAP)-dependent promoters are shown with red double-arrows labelled with −35 and −10, and the phage RNAP-dependent promoters with black arrows labelled from P1 to P12. Terminators are shown along the genome as purple triangles and labelled from T1 to T4. The genetic map was created using the Geneious software.

    Article Snippet: The kinase was then inactivated at 75 °C for 15 min. Ligation of the phosphorylated DNA to a 500 bp fragment of the fliC gene of Y. enterocolitica O:3 was performed in a 30 µL reaction mixture containing ~400 ng of the phosphorylated DNA, ~40 ng of fliC gene fragment, 2 µL of 50% ( w / v ) PEG 4000 (Thermo Scientific), 3 µL of 10 × T4 µL DNA Ligase buffer and 1 µL T4 DNA ligase (New England BioLabs).

    Techniques: Lysis, Software