5hmc antibody  (Zymo Research)


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    Name:
    Anti 5 Hydroxymethylcytosine Polyclonal Antibody
    Description:
    The rabbit Anti 5 hmC Polyclonal Antibody can robustly distinguish between hydroxymethylated DNA and methylated or unmodified DNA with limited to no cross reactivity The antibody has been validated in ELISA and immunoprecipitation based enrichment assays and is suitable for use in further applications including immunohistochemical labeling and chromatographic blotting
    Catalog Number:
    a4001-200
    Product Aliases:
    Anti-5hmC Monoclonal Antibody
    Price:
    None
    Applications:
    ELISA, IHC, Western Blot
    Size:
    200 ug
    Category:
    Life Science Antibodies
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    Structured Review

    Zymo Research 5hmc antibody
    The rabbit Anti 5 hmC Polyclonal Antibody can robustly distinguish between hydroxymethylated DNA and methylated or unmodified DNA with limited to no cross reactivity The antibody has been validated in ELISA and immunoprecipitation based enrichment assays and is suitable for use in further applications including immunohistochemical labeling and chromatographic blotting
    https://www.bioz.com/result/5hmc antibody/product/Zymo Research
    Average 94 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    5hmc antibody - by Bioz Stars, 2020-10
    94/100 stars

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    Related Articles

    Amplification:

    Article Title: Anabolic and Antiresorptive Modulation of Bone Homeostasis by the Epigenetic Modulator Sulforaphane, a Naturally Occurring Isothiocyanate *
    Article Snippet: .. For assessment of the specificity of the 5hmC antibody, either 40 ng of non-methylated, fully methylated, or fully hydroxymethylated DNA standards (Zymo Research) were run in parallel and amplified by real time PCR using the supplied PCR Primers. .. Analysis of SFN in mice was based on a protocol published previously by Kong et al. ( ).

    Methylation:

    Article Title: Anabolic and Antiresorptive Modulation of Bone Homeostasis by the Epigenetic Modulator Sulforaphane, a Naturally Occurring Isothiocyanate *
    Article Snippet: .. For assessment of the specificity of the 5hmC antibody, either 40 ng of non-methylated, fully methylated, or fully hydroxymethylated DNA standards (Zymo Research) were run in parallel and amplified by real time PCR using the supplied PCR Primers. .. Analysis of SFN in mice was based on a protocol published previously by Kong et al. ( ).

    DNA Methylation Assay:

    Article Title: Association of intronic DNA methylation and hydroxymethylation alterations in the epigenetic etiology of dilated cardiomyopathy
    Article Snippet: .. Hydroxymethylated DNA immunoprecipitation (HMeDIP)-Seq libraries were prepared by using components from the DNA Methylation IP Kit (D5101) with anti-5hmC antibody (A4001-25). .. Libraries were prepared using the Pico Methyl-Seq Library Prep Kit (D5455) by skipping the bisulfite conversion.

    Real-time Polymerase Chain Reaction:

    Article Title: Anabolic and Antiresorptive Modulation of Bone Homeostasis by the Epigenetic Modulator Sulforaphane, a Naturally Occurring Isothiocyanate *
    Article Snippet: .. For assessment of the specificity of the 5hmC antibody, either 40 ng of non-methylated, fully methylated, or fully hydroxymethylated DNA standards (Zymo Research) were run in parallel and amplified by real time PCR using the supplied PCR Primers. .. Analysis of SFN in mice was based on a protocol published previously by Kong et al. ( ).

    Immunoprecipitation:

    Article Title: Association of intronic DNA methylation and hydroxymethylation alterations in the epigenetic etiology of dilated cardiomyopathy
    Article Snippet: .. Hydroxymethylated DNA immunoprecipitation (HMeDIP)-Seq libraries were prepared by using components from the DNA Methylation IP Kit (D5101) with anti-5hmC antibody (A4001-25). .. Libraries were prepared using the Pico Methyl-Seq Library Prep Kit (D5455) by skipping the bisulfite conversion.

    Polymerase Chain Reaction:

    Article Title: Anabolic and Antiresorptive Modulation of Bone Homeostasis by the Epigenetic Modulator Sulforaphane, a Naturally Occurring Isothiocyanate *
    Article Snippet: .. For assessment of the specificity of the 5hmC antibody, either 40 ng of non-methylated, fully methylated, or fully hydroxymethylated DNA standards (Zymo Research) were run in parallel and amplified by real time PCR using the supplied PCR Primers. .. Analysis of SFN in mice was based on a protocol published previously by Kong et al. ( ).

    Methylated DNA Immunoprecipitation:

    Article Title: Breeding scheme and maternal small RNAs affect the efficiency of transgenerational inheritance of a paramutation in mice
    Article Snippet: .. For 5 hmC MeDIP, the same protocol was followed, but the 5 hmC antibody (Zymo, Cat. #A4001-25), was used in place of the 5 mC antibody, for the pull-down. ..

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  • 94
    Zymo Research 5hmc antibody
    5hmc Antibody, supplied by Zymo Research, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5hmc antibody/product/Zymo Research
    Average 94 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    5hmc antibody - by Bioz Stars, 2020-10
    94/100 stars
      Buy from Supplier

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