anti 5 methylcytosine polyclonal antibody  (Zymo Research)


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    Name:
    Anti 5 Hydroxymethylcytosine Polyclonal Antibody
    Description:
    The rabbit Anti 5 hmC Polyclonal Antibody can robustly distinguish between hydroxymethylated DNA and methylated or unmodified DNA with limited to no cross reactivity The antibody has been validated in ELISA and immunoprecipitation based enrichment assays and is suitable for use in further applications including immunohistochemical labeling and chromatographic blotting
    Catalog Number:
    a4001-200
    Product Aliases:
    Anti-5hmC Monoclonal Antibody
    Price:
    None
    Applications:
    ELISA, IHC, Western Blot
    Size:
    200 ug
    Category:
    Life Science Antibodies
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    Structured Review

    Zymo Research anti 5 methylcytosine polyclonal antibody
    The rabbit Anti 5 hmC Polyclonal Antibody can robustly distinguish between hydroxymethylated DNA and methylated or unmodified DNA with limited to no cross reactivity The antibody has been validated in ELISA and immunoprecipitation based enrichment assays and is suitable for use in further applications including immunohistochemical labeling and chromatographic blotting
    https://www.bioz.com/result/anti 5 methylcytosine polyclonal antibody/product/Zymo Research
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    anti 5 methylcytosine polyclonal antibody - by Bioz Stars, 2020-02
    92/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Anabolic and Antiresorptive Modulation of Bone Homeostasis by the Epigenetic Modulator Sulforaphane, a Naturally Occurring Isothiocyanate *
    Article Snippet: .. For assessment of the specificity of the 5hmC antibody, either 40 ng of non-methylated, fully methylated, or fully hydroxymethylated DNA standards (Zymo Research) were run in parallel and amplified by real time PCR using the supplied PCR Primers. .. Analysis of SFN in mice was based on a protocol published previously by Kong et al. ( ).

    Amplification:

    Article Title: Anabolic and Antiresorptive Modulation of Bone Homeostasis by the Epigenetic Modulator Sulforaphane, a Naturally Occurring Isothiocyanate *
    Article Snippet: .. For assessment of the specificity of the 5hmC antibody, either 40 ng of non-methylated, fully methylated, or fully hydroxymethylated DNA standards (Zymo Research) were run in parallel and amplified by real time PCR using the supplied PCR Primers. .. Analysis of SFN in mice was based on a protocol published previously by Kong et al. ( ).

    Methylation:

    Article Title: Anabolic and Antiresorptive Modulation of Bone Homeostasis by the Epigenetic Modulator Sulforaphane, a Naturally Occurring Isothiocyanate *
    Article Snippet: .. For assessment of the specificity of the 5hmC antibody, either 40 ng of non-methylated, fully methylated, or fully hydroxymethylated DNA standards (Zymo Research) were run in parallel and amplified by real time PCR using the supplied PCR Primers. .. Analysis of SFN in mice was based on a protocol published previously by Kong et al. ( ).

    Magnetic Beads:

    Article Title: Anabolic and Antiresorptive Modulation of Bone Homeostasis by the Epigenetic Modulator Sulforaphane, a Naturally Occurring Isothiocyanate *
    Article Snippet: DNA was eluted from the magnetic beads with 75 μl of 2 m NaCl solution. .. For assessment of the specificity of the 5hmC antibody, either 40 ng of non-methylated, fully methylated, or fully hydroxymethylated DNA standards (Zymo Research) were run in parallel and amplified by real time PCR using the supplied PCR Primers.

    Cell Culture:

    Article Title: Specific functions of TET1 and TET2 in regulating mesenchymal cell lineage determination
    Article Snippet: Briefly, 96 well plates were coated with either anti-5-hydroxymethylcytosine polyclonal antibody or anti-5-methylcytosine polyclonal antibody (Zymo Research) (1 ng/μl) in coating buffer. .. BMSC were cultured for 2 weeks under normal, osteogenic or adipogenic inductive conditions.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Specific functions of TET1 and TET2 in regulating mesenchymal cell lineage determination
    Article Snippet: Global 5hmc and 5mc analysis The levels of 5mc and 5hmc were assayed by ELISA using either the Quest 5-hmc DNA ELISA Kit (Cat# D5425, Zymo Research) or 5-mc DNA ELISA Kit (cat# D5325, Zymo Research), respectively. .. Briefly, 96 well plates were coated with either anti-5-hydroxymethylcytosine polyclonal antibody or anti-5-methylcytosine polyclonal antibody (Zymo Research) (1 ng/μl) in coating buffer.

    Immunoprecipitation:

    Article Title: Anabolic and Antiresorptive Modulation of Bone Homeostasis by the Epigenetic Modulator Sulforaphane, a Naturally Occurring Isothiocyanate *
    Article Snippet: After DNA purification, hydroxymethylated DNA fragments were extracted by immunoprecipitation as described previously ( ). .. For assessment of the specificity of the 5hmC antibody, either 40 ng of non-methylated, fully methylated, or fully hydroxymethylated DNA standards (Zymo Research) were run in parallel and amplified by real time PCR using the supplied PCR Primers.

    Incubation:

    Article Title: Specific functions of TET1 and TET2 in regulating mesenchymal cell lineage determination
    Article Snippet: Briefly, 96 well plates were coated with either anti-5-hydroxymethylcytosine polyclonal antibody or anti-5-methylcytosine polyclonal antibody (Zymo Research) (1 ng/μl) in coating buffer. .. 100 ng of denatured DNA and standard DNA with known 5hmc or 5mc concentrations was added to the wells and incubated for 1 h. After washing, the cells were incubated with anti-DNA HRP conjugated antibody for 30 min. Wells were washed and HRP developer was added.

    DNA Purification:

    Article Title: Anabolic and Antiresorptive Modulation of Bone Homeostasis by the Epigenetic Modulator Sulforaphane, a Naturally Occurring Isothiocyanate *
    Article Snippet: After DNA purification, hydroxymethylated DNA fragments were extracted by immunoprecipitation as described previously ( ). .. For assessment of the specificity of the 5hmC antibody, either 40 ng of non-methylated, fully methylated, or fully hydroxymethylated DNA standards (Zymo Research) were run in parallel and amplified by real time PCR using the supplied PCR Primers.

    Polymerase Chain Reaction:

    Article Title: Anabolic and Antiresorptive Modulation of Bone Homeostasis by the Epigenetic Modulator Sulforaphane, a Naturally Occurring Isothiocyanate *
    Article Snippet: .. For assessment of the specificity of the 5hmC antibody, either 40 ng of non-methylated, fully methylated, or fully hydroxymethylated DNA standards (Zymo Research) were run in parallel and amplified by real time PCR using the supplied PCR Primers. .. Analysis of SFN in mice was based on a protocol published previously by Kong et al. ( ).

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  • 92
    Zymo Research anti 5 methylcytosine polyclonal antibody
    Anti 5 Methylcytosine Polyclonal Antibody, supplied by Zymo Research, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti 5 methylcytosine polyclonal antibody/product/Zymo Research
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    anti 5 methylcytosine polyclonal antibody - by Bioz Stars, 2020-02
    92/100 stars
      Buy from Supplier

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