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    Structured Review

    Boster Bio dapi
    Notch3 regulates cardiac fibroblast (CF) function via the RhoA/ROCK/Hif1α pathway. (A) CFs were pretreated with 2-ME for 2 h before notch3 knockdown. CF proliferation was quantified using the <t>EdU</t> assay ( n = 6). Scale bars = 200 μm. (B) The Cell Counting Kit-8 (CCK8) assay was also used to determine CF proliferation (above, n = 3). Quantification of CF proliferation determined by EdU assay (below, n = 6). (C) Western blot analysis of cleaved caspase-3, total caspase3, and Bcl2 in different groups. The quantification of the protein bands is shown on the right ( n = 3). (D) CFs were pretreated with DMOG for 2 h before notch3 overexpression. Flow cytometry quantified the percentage of apoptosis in each group ( n = 3). (E) Western blot analysis of α-SMA, Col I, and Col III in CFs pretreated with 2-ME for 2 h before notch3 knockdown. The quantification of the protein bands is shown below ( n = 3). (F) Representative Western blot and quantification of Hif1α in CFs pretreated with Y-27632 for 2 h before notch3 knockdown ( n = 3). (G) Representative immunofluorescence images and quantification of Hif1α (red) in CFs pretreated with Y-27632 for 2 h before notch3 knockdown ( n = 6). Nuclei were detected with <t>DAPI</t> (blue). Scale bars = 200 μm. Values represent the mean ± SD. * P
    Dapi, supplied by Boster Bio, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dapi/product/Boster Bio
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dapi - by Bioz Stars, 2022-07
    97/100 stars

    Images

    1) Product Images from "Notch3 Modulates Cardiac Fibroblast Proliferation, Apoptosis, and Fibroblast to Myofibroblast Transition via Negative Regulation of the RhoA/ROCK/Hif1α Axis"

    Article Title: Notch3 Modulates Cardiac Fibroblast Proliferation, Apoptosis, and Fibroblast to Myofibroblast Transition via Negative Regulation of the RhoA/ROCK/Hif1α Axis

    Journal: Frontiers in Physiology

    doi: 10.3389/fphys.2020.00669

    Notch3 regulates cardiac fibroblast (CF) function via the RhoA/ROCK/Hif1α pathway. (A) CFs were pretreated with 2-ME for 2 h before notch3 knockdown. CF proliferation was quantified using the EdU assay ( n = 6). Scale bars = 200 μm. (B) The Cell Counting Kit-8 (CCK8) assay was also used to determine CF proliferation (above, n = 3). Quantification of CF proliferation determined by EdU assay (below, n = 6). (C) Western blot analysis of cleaved caspase-3, total caspase3, and Bcl2 in different groups. The quantification of the protein bands is shown on the right ( n = 3). (D) CFs were pretreated with DMOG for 2 h before notch3 overexpression. Flow cytometry quantified the percentage of apoptosis in each group ( n = 3). (E) Western blot analysis of α-SMA, Col I, and Col III in CFs pretreated with 2-ME for 2 h before notch3 knockdown. The quantification of the protein bands is shown below ( n = 3). (F) Representative Western blot and quantification of Hif1α in CFs pretreated with Y-27632 for 2 h before notch3 knockdown ( n = 3). (G) Representative immunofluorescence images and quantification of Hif1α (red) in CFs pretreated with Y-27632 for 2 h before notch3 knockdown ( n = 6). Nuclei were detected with DAPI (blue). Scale bars = 200 μm. Values represent the mean ± SD. * P
    Figure Legend Snippet: Notch3 regulates cardiac fibroblast (CF) function via the RhoA/ROCK/Hif1α pathway. (A) CFs were pretreated with 2-ME for 2 h before notch3 knockdown. CF proliferation was quantified using the EdU assay ( n = 6). Scale bars = 200 μm. (B) The Cell Counting Kit-8 (CCK8) assay was also used to determine CF proliferation (above, n = 3). Quantification of CF proliferation determined by EdU assay (below, n = 6). (C) Western blot analysis of cleaved caspase-3, total caspase3, and Bcl2 in different groups. The quantification of the protein bands is shown on the right ( n = 3). (D) CFs were pretreated with DMOG for 2 h before notch3 overexpression. Flow cytometry quantified the percentage of apoptosis in each group ( n = 3). (E) Western blot analysis of α-SMA, Col I, and Col III in CFs pretreated with 2-ME for 2 h before notch3 knockdown. The quantification of the protein bands is shown below ( n = 3). (F) Representative Western blot and quantification of Hif1α in CFs pretreated with Y-27632 for 2 h before notch3 knockdown ( n = 3). (G) Representative immunofluorescence images and quantification of Hif1α (red) in CFs pretreated with Y-27632 for 2 h before notch3 knockdown ( n = 6). Nuclei were detected with DAPI (blue). Scale bars = 200 μm. Values represent the mean ± SD. * P

    Techniques Used: EdU Assay, Cell Counting, CCK-8 Assay, Western Blot, Over Expression, Flow Cytometry, Immunofluorescence

    2) Product Images from "Knockdown of ELMO3 Suppresses Growth, Invasion and Metastasis of Colorectal Cancer"

    Article Title: Knockdown of ELMO3 Suppresses Growth, Invasion and Metastasis of Colorectal Cancer

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms17122119

    Knockdown of ELMO3 inhibited the F-actin polymerization of HCT116 cells. The cytoskeleton and nuclei were stained with FITC-phalloidin and DAPI. The fluorescent signals of F-actin, labeled white arrows, were detected on the plasma membrane in the NC and Control groups. The representative photographs are shown at 400× magnification.
    Figure Legend Snippet: Knockdown of ELMO3 inhibited the F-actin polymerization of HCT116 cells. The cytoskeleton and nuclei were stained with FITC-phalloidin and DAPI. The fluorescent signals of F-actin, labeled white arrows, were detected on the plasma membrane in the NC and Control groups. The representative photographs are shown at 400× magnification.

    Techniques Used: Staining, Labeling

    3) Product Images from "Anti-c-Met antibody bioconjugated with hollow gold nanospheres as a novel nanomaterial for targeted radiation ablation of human cervical cancer cell"

    Article Title: Anti-c-Met antibody bioconjugated with hollow gold nanospheres as a novel nanomaterial for targeted radiation ablation of human cervical cancer cell

    Journal: Oncology Letters

    doi: 10.3892/ol.2017.6383

    Characteristics of HGNs and CaSki cells. (A) Transmission electron microscope images of HGNs. (B) The resonance peak of HGNs shifts from 733 nm to 837 nm following modification with the anti-c-Met antibody. (C) The numbers of nanospheres endocytosed by each cell was counted by inductively coupled plasma atomic emission spectroscopy. The average numbers of the nanoparticles internalized per CaSki cell was 5,378±401 for naked HGNs and 8,681±742 for anti-c-Met/HGNs, respectively (P=0.005) at 24 h. (D) Immunofluorescence demonstrated that CaSki cells overexpressed c-Met on cell membrane (green staining); DAPI (blue) stains the nucleus. HGN, hollow gold nanoparticle; anti-c-Met, MET proto-oncogene, receptor tyrosine kinase antibodies; DAPI, 4′,6-diamidino-2-phenylindole.
    Figure Legend Snippet: Characteristics of HGNs and CaSki cells. (A) Transmission electron microscope images of HGNs. (B) The resonance peak of HGNs shifts from 733 nm to 837 nm following modification with the anti-c-Met antibody. (C) The numbers of nanospheres endocytosed by each cell was counted by inductively coupled plasma atomic emission spectroscopy. The average numbers of the nanoparticles internalized per CaSki cell was 5,378±401 for naked HGNs and 8,681±742 for anti-c-Met/HGNs, respectively (P=0.005) at 24 h. (D) Immunofluorescence demonstrated that CaSki cells overexpressed c-Met on cell membrane (green staining); DAPI (blue) stains the nucleus. HGN, hollow gold nanoparticle; anti-c-Met, MET proto-oncogene, receptor tyrosine kinase antibodies; DAPI, 4′,6-diamidino-2-phenylindole.

    Techniques Used: Transmission Assay, Microscopy, Modification, Spectroscopy, Immunofluorescence, Staining

    4) Product Images from "Effects of miR-101-3p on goat granulosa cells in vitro and ovarian development in vivo via STC1"

    Article Title: Effects of miR-101-3p on goat granulosa cells in vitro and ovarian development in vivo via STC1

    Journal: Journal of Animal Science and Biotechnology

    doi: 10.1186/s40104-020-00506-6

    Effects of miR-101-3p and STC1 on ovarian development in mice. Twelve mice are divided into four groups and treated with NC (100 μL saline), miR-101-3p agonists (miR-101-3p-ag; 10 nmol per time, with 100 μL saline), miR-101-3p antagonist (miR-101-3p-antag; 20 nmol per time, with 100 μL saline) or siRNA-STC1 (si-STC1; 20 nmol per time, with 100 μL saline). a FISH is performed to identify the distribution and expression levels of miR-101-3p in mouse ovaries. The nucleus stained by DAPI is blue under ultraviolet excitation, and the positive expression is green fluorescence of the corresponding fluorescein-labelled FAM. The red arrows represent a small amount of fluorescence of miR-101-3p. b Immunohistochemistry exhibits the expressions of STC1 in dissected mouse ovaries. SABC-positive cells are dyed in brown, while cell nuclei are dyed with DAPI in blue. The area of positive cells (Area), mean optical density (Mean Density), and integrated optical density (IOD) are effective indicators for semi-quantitative analysis of immunohistochemistry results. c The mouse ovarian morphology is observed using HE staining. The sections are stained with hematoxylin in blue purple, and stained with eosin in red. d After HE staining, the follicles are counted at each stage. Follicle counting principles are as follows: primordial follicles: oocytes surrounded by a layer of flat granulosa cells or mixed cells of flat and cubic granulosa cells (total cell number
    Figure Legend Snippet: Effects of miR-101-3p and STC1 on ovarian development in mice. Twelve mice are divided into four groups and treated with NC (100 μL saline), miR-101-3p agonists (miR-101-3p-ag; 10 nmol per time, with 100 μL saline), miR-101-3p antagonist (miR-101-3p-antag; 20 nmol per time, with 100 μL saline) or siRNA-STC1 (si-STC1; 20 nmol per time, with 100 μL saline). a FISH is performed to identify the distribution and expression levels of miR-101-3p in mouse ovaries. The nucleus stained by DAPI is blue under ultraviolet excitation, and the positive expression is green fluorescence of the corresponding fluorescein-labelled FAM. The red arrows represent a small amount of fluorescence of miR-101-3p. b Immunohistochemistry exhibits the expressions of STC1 in dissected mouse ovaries. SABC-positive cells are dyed in brown, while cell nuclei are dyed with DAPI in blue. The area of positive cells (Area), mean optical density (Mean Density), and integrated optical density (IOD) are effective indicators for semi-quantitative analysis of immunohistochemistry results. c The mouse ovarian morphology is observed using HE staining. The sections are stained with hematoxylin in blue purple, and stained with eosin in red. d After HE staining, the follicles are counted at each stage. Follicle counting principles are as follows: primordial follicles: oocytes surrounded by a layer of flat granulosa cells or mixed cells of flat and cubic granulosa cells (total cell number

    Techniques Used: Mouse Assay, Fluorescence In Situ Hybridization, Expressing, Staining, Fluorescence, Immunohistochemistry

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    Boster Bio dapi 4 6 diamidino 2 phenylindole dihydrochloride for nucleic acid staining
    Identification of LFs and proliferation assays. (A) Immunocytochemical staining of LFs from control rats. Primary LFs were stained with vimentin. LFs exhibited stellate morphology, with an elongated spindle-like structure. Scale bar, 100 µm. (B) Immunofluorescence staining of LFs. Primary LFs from control rats were labeled with vimentin-specific antibodies (green) and cell nuclei were labeled with <t>4′,6-diamidino-2-phenylindole</t> (blue). Scale bar, 50 µm. (C) Cell-cycle distribution. Cell populations in G0/G1, S and G2/M phases were determined by calculating the mean of five independent experiments. The proportion of cells in the G0/G1 phase decreased, associated with an increase in the S phase in LFs from neonatal rats in the hyperoxia group at postnatal day 7 and 14 compared with the room air control group (n=5). (D) Effect of hyperoxia on cell proliferation. CCK-8 assays were used to measure proliferation. Hyperoxia promoted cell proliferation at postnatal day 7 and 14 (n=6). (E) Col-I secreted protein levels in LFs determined by ELISA. An increase was observed in the hyperoxia group compared with the room air control group (n=5). *P
    Dapi 4 6 Diamidino 2 Phenylindole Dihydrochloride For Nucleic Acid Staining, supplied by Boster Bio, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Identification of LFs and proliferation assays. (A) Immunocytochemical staining of LFs from control rats. Primary LFs were stained with vimentin. LFs exhibited stellate morphology, with an elongated spindle-like structure. Scale bar, 100 µm. (B) Immunofluorescence staining of LFs. Primary LFs from control rats were labeled with vimentin-specific antibodies (green) and cell nuclei were labeled with 4′,6-diamidino-2-phenylindole (blue). Scale bar, 50 µm. (C) Cell-cycle distribution. Cell populations in G0/G1, S and G2/M phases were determined by calculating the mean of five independent experiments. The proportion of cells in the G0/G1 phase decreased, associated with an increase in the S phase in LFs from neonatal rats in the hyperoxia group at postnatal day 7 and 14 compared with the room air control group (n=5). (D) Effect of hyperoxia on cell proliferation. CCK-8 assays were used to measure proliferation. Hyperoxia promoted cell proliferation at postnatal day 7 and 14 (n=6). (E) Col-I secreted protein levels in LFs determined by ELISA. An increase was observed in the hyperoxia group compared with the room air control group (n=5). *P

    Journal: Experimental and Therapeutic Medicine

    Article Title: Association of the proliferation of lung fibroblasts with the ERK1/2 signaling pathway in neonatal rats with hyperoxia-induced lung fibrosis

    doi: 10.3892/etm.2018.6999

    Figure Lengend Snippet: Identification of LFs and proliferation assays. (A) Immunocytochemical staining of LFs from control rats. Primary LFs were stained with vimentin. LFs exhibited stellate morphology, with an elongated spindle-like structure. Scale bar, 100 µm. (B) Immunofluorescence staining of LFs. Primary LFs from control rats were labeled with vimentin-specific antibodies (green) and cell nuclei were labeled with 4′,6-diamidino-2-phenylindole (blue). Scale bar, 50 µm. (C) Cell-cycle distribution. Cell populations in G0/G1, S and G2/M phases were determined by calculating the mean of five independent experiments. The proportion of cells in the G0/G1 phase decreased, associated with an increase in the S phase in LFs from neonatal rats in the hyperoxia group at postnatal day 7 and 14 compared with the room air control group (n=5). (D) Effect of hyperoxia on cell proliferation. CCK-8 assays were used to measure proliferation. Hyperoxia promoted cell proliferation at postnatal day 7 and 14 (n=6). (E) Col-I secreted protein levels in LFs determined by ELISA. An increase was observed in the hyperoxia group compared with the room air control group (n=5). *P

    Article Snippet: LFs were stained using 50 µl DAPI solution (1:100; cat. no. AR1176; Boster Biological Technology, Pleasanton, CA, USA) for 10 min at 37°C and a fluorescence microscope (magnification, ×400) was used to analyze the results.

    Techniques: Staining, Immunofluorescence, Labeling, CCK-8 Assay, Enzyme-linked Immunosorbent Assay

    Notch3 regulates cardiac fibroblast (CF) function via the RhoA/ROCK/Hif1α pathway. (A) CFs were pretreated with 2-ME for 2 h before notch3 knockdown. CF proliferation was quantified using the EdU assay ( n = 6). Scale bars = 200 μm. (B) The Cell Counting Kit-8 (CCK8) assay was also used to determine CF proliferation (above, n = 3). Quantification of CF proliferation determined by EdU assay (below, n = 6). (C) Western blot analysis of cleaved caspase-3, total caspase3, and Bcl2 in different groups. The quantification of the protein bands is shown on the right ( n = 3). (D) CFs were pretreated with DMOG for 2 h before notch3 overexpression. Flow cytometry quantified the percentage of apoptosis in each group ( n = 3). (E) Western blot analysis of α-SMA, Col I, and Col III in CFs pretreated with 2-ME for 2 h before notch3 knockdown. The quantification of the protein bands is shown below ( n = 3). (F) Representative Western blot and quantification of Hif1α in CFs pretreated with Y-27632 for 2 h before notch3 knockdown ( n = 3). (G) Representative immunofluorescence images and quantification of Hif1α (red) in CFs pretreated with Y-27632 for 2 h before notch3 knockdown ( n = 6). Nuclei were detected with DAPI (blue). Scale bars = 200 μm. Values represent the mean ± SD. * P

    Journal: Frontiers in Physiology

    Article Title: Notch3 Modulates Cardiac Fibroblast Proliferation, Apoptosis, and Fibroblast to Myofibroblast Transition via Negative Regulation of the RhoA/ROCK/Hif1α Axis

    doi: 10.3389/fphys.2020.00669

    Figure Lengend Snippet: Notch3 regulates cardiac fibroblast (CF) function via the RhoA/ROCK/Hif1α pathway. (A) CFs were pretreated with 2-ME for 2 h before notch3 knockdown. CF proliferation was quantified using the EdU assay ( n = 6). Scale bars = 200 μm. (B) The Cell Counting Kit-8 (CCK8) assay was also used to determine CF proliferation (above, n = 3). Quantification of CF proliferation determined by EdU assay (below, n = 6). (C) Western blot analysis of cleaved caspase-3, total caspase3, and Bcl2 in different groups. The quantification of the protein bands is shown on the right ( n = 3). (D) CFs were pretreated with DMOG for 2 h before notch3 overexpression. Flow cytometry quantified the percentage of apoptosis in each group ( n = 3). (E) Western blot analysis of α-SMA, Col I, and Col III in CFs pretreated with 2-ME for 2 h before notch3 knockdown. The quantification of the protein bands is shown below ( n = 3). (F) Representative Western blot and quantification of Hif1α in CFs pretreated with Y-27632 for 2 h before notch3 knockdown ( n = 3). (G) Representative immunofluorescence images and quantification of Hif1α (red) in CFs pretreated with Y-27632 for 2 h before notch3 knockdown ( n = 6). Nuclei were detected with DAPI (blue). Scale bars = 200 μm. Values represent the mean ± SD. * P

    Article Snippet: The CFs were seeded in 24-well plate, transfected with si notch3 or ov-N3ICD plasmid for 48 h, and incubated with 10 μm EdU for 24 h. We then fixed the cells with 4% paraformaldehyde, permeabilized them with 0.5% Triton-X 100 in phosphate-buffered saline (PBS), stained with EdU, and then counterstained with DAPI (Boster, China).

    Techniques: EdU Assay, Cell Counting, CCK-8 Assay, Western Blot, Over Expression, Flow Cytometry, Immunofluorescence

    Identification of LFs and proliferation assays. (A) Immunocytochemical staining of LFs from control rats. Primary LFs were stained with vimentin. LFs exhibited stellate morphology, with an elongated spindle-like structure. Scale bar, 100 µm. (B) Immunofluorescence staining of LFs. Primary LFs from control rats were labeled with vimentin-specific antibodies (green) and cell nuclei were labeled with 4′,6-diamidino-2-phenylindole (blue). Scale bar, 50 µm. (C) Cell-cycle distribution. Cell populations in G0/G1, S and G2/M phases were determined by calculating the mean of five independent experiments. The proportion of cells in the G0/G1 phase decreased, associated with an increase in the S phase in LFs from neonatal rats in the hyperoxia group at postnatal day 7 and 14 compared with the room air control group (n=5). (D) Effect of hyperoxia on cell proliferation. CCK-8 assays were used to measure proliferation. Hyperoxia promoted cell proliferation at postnatal day 7 and 14 (n=6). (E) Col-I secreted protein levels in LFs determined by ELISA. An increase was observed in the hyperoxia group compared with the room air control group (n=5). *P

    Journal: Experimental and Therapeutic Medicine

    Article Title: Association of the proliferation of lung fibroblasts with the ERK1/2 signaling pathway in neonatal rats with hyperoxia-induced lung fibrosis

    doi: 10.3892/etm.2018.6999

    Figure Lengend Snippet: Identification of LFs and proliferation assays. (A) Immunocytochemical staining of LFs from control rats. Primary LFs were stained with vimentin. LFs exhibited stellate morphology, with an elongated spindle-like structure. Scale bar, 100 µm. (B) Immunofluorescence staining of LFs. Primary LFs from control rats were labeled with vimentin-specific antibodies (green) and cell nuclei were labeled with 4′,6-diamidino-2-phenylindole (blue). Scale bar, 50 µm. (C) Cell-cycle distribution. Cell populations in G0/G1, S and G2/M phases were determined by calculating the mean of five independent experiments. The proportion of cells in the G0/G1 phase decreased, associated with an increase in the S phase in LFs from neonatal rats in the hyperoxia group at postnatal day 7 and 14 compared with the room air control group (n=5). (D) Effect of hyperoxia on cell proliferation. CCK-8 assays were used to measure proliferation. Hyperoxia promoted cell proliferation at postnatal day 7 and 14 (n=6). (E) Col-I secreted protein levels in LFs determined by ELISA. An increase was observed in the hyperoxia group compared with the room air control group (n=5). *P

    Article Snippet: LFs were stained using 50 µl DAPI solution (1:100; cat. no. AR1176; Boster Biological Technology, Pleasanton, CA, USA) for 10 min at 37°C and a fluorescence microscope (magnification, ×400) was used to analyze the results.

    Techniques: Staining, Immunofluorescence, Labeling, CCK-8 Assay, Enzyme-linked Immunosorbent Assay

    Knockdown of ELMO3 inhibited the F-actin polymerization of HCT116 cells. The cytoskeleton and nuclei were stained with FITC-phalloidin and DAPI. The fluorescent signals of F-actin, labeled white arrows, were detected on the plasma membrane in the NC and Control groups. The representative photographs are shown at 400× magnification.

    Journal: International Journal of Molecular Sciences

    Article Title: Knockdown of ELMO3 Suppresses Growth, Invasion and Metastasis of Colorectal Cancer

    doi: 10.3390/ijms17122119

    Figure Lengend Snippet: Knockdown of ELMO3 inhibited the F-actin polymerization of HCT116 cells. The cytoskeleton and nuclei were stained with FITC-phalloidin and DAPI. The fluorescent signals of F-actin, labeled white arrows, were detected on the plasma membrane in the NC and Control groups. The representative photographs are shown at 400× magnification.

    Article Snippet: The cells were then stained with 1 mM FITC-phalloidin (Sigma) for 40 min in the dark at room temperature to visualize the actin cytoskeleton, and counterstained with 50 mM DAPI (Boster, Wuhan, China) for 2 min in the dark at room temperature to visualize cell nuclei.

    Techniques: Staining, Labeling