hcn1 (Alomone Labs)
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Structured Review
Alomone Labs
hcn1
Hcn1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hcn1/product/Alomone Labs
Average 90 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Hcn1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hcn1/product/Alomone Labs
Average 90 stars, based on 1 article reviews
Price from $9.99 to $1999.99
hcn1 - by Bioz Stars,
2022-06
90/100 stars
Images
1) Product Images from "Differential Distribution and Function of Hyperpolarization-Activated Channels in Sensory Neurons and Mechanosensitive Fibers"
Article Title: Differential Distribution and Function of Hyperpolarization-Activated Channels in Sensory Neurons and Mechanosensitive Fibers
Journal: The Journal of Neuroscience
doi: 10.1523/JNEUROSCI.5156-03.2004
Figure Legend Snippet: HCN immunoreactivity in club endings of myelinated fibers accompanied by fine unmyelinated fibers. Left, HCN1 (FITC) colocalized (yellow) with peripherin (Rhodamine Red-X) in a club ending but not with fine unmyelinated fiber (arrowhead). Center, HCN2 (Rhodamine Red-X) appeared in both the fine unmyelinated fibers and in a club ending of a myelinated fiber (yellow). Right, Both club endings and fine fibers (arrowhead) labeled with FITC-NF mixture also expressed HCN4 (Rhodamine Red-X). HCN2 and HCN4 (Rhodamine Red-X) are also found in cells surrounding the fibers.
Techniques Used: Labeling
Figure Legend Snippet: HCN1, HCN2, and HCN4 immunoreactivity in nodose neurons. A–C , HCN immunoreactivity identified in 6–10 μm sections of nodose ganglion. HCN1 immunoreactivity was localized to a small subpopulation of neurons and, in most of these cells, was heavily localized at the plasma membrane ( A ). HCN2 ( B ) and HCN4 ( C ) immunoreactivity was present in all neurons in the ganglion. D–F , Single confocal sections through cultured nodose neurons selected for expression of HCN1 ( D ), HCN2 ( E ), and HCN4 ( F ). Heavy labeling at the membrane is again shown for HCN1 ( D ). Patches of HCN2 and HCN4 immunoreactivity were located at the cell perimeter; examples are indicated by the arrows ( E, F ). The light microscopic differential interference contrast image is also shown for each neuron. The calibration bar in C also applies to A and B , whereas calibration in F applies to E . The antibodies were preabsorbed with the immunizing peptide as shown in the figure. A control for nonspecific staining omitted the primary Ab (data not shown).
Techniques Used: Cell Culture, Expressing, Labeling, Staining
Figure Legend Snippet: Colocalization of HCN1 and IB4 or VR1. A , No dose ganglion section immunolabeled with rabbit HCN1 Ab (left) and IB4 lectin (right). HCN1 neurons do not contain IB4. Three of the HCN1-labeled neurons are identified by an asterisk. B , Nodose section labeled with anti-HCN1 Ab (left) and anti-VR1 Ab (center) is shown overlaid on the right. The neurons with strong labeling for HCN1 did not coexpress VR1, but weaker HCN1 staining is seen on two VR1(*)-immunoreactive neurons. The arrow indicates an example of HCN1 axonal labeling.
Techniques Used: Immunolabeling, Labeling, Staining
Figure Legend Snippet: HCN1, HCN2, HCN3, and HCN4 mRNA is expressed in nodose ganglia. PCR products resulting from the amplification of first-strand cDNA prepared with (+) or without (–) RT from nodose ganglia or brain poly A+ RNA with HCN1-, HCN2-, HCN3-, and HCN4-specific oligonucleotides were separated by electrophoresis and transferred to nylon membranes (Ambion). After Southern hybridization with 32 P-labeled specific internal oligomers, the autoradiogram showed a positive signal for all four channels from nodose and rat brain in the (+) RT lanes and no signals in the control (–) RT. The oligonucleotide probes amplify cDNA of 641 bp for HCN1, 638 bp for HCN2, 509 bp for HCN3, and 635 bp for HCN4.
Techniques Used: Polymerase Chain Reaction, Amplification, Electrophoresis, Hybridization, Labeling
Figure Legend Snippet: HCN immunoreactivity in aortic baroreceptor terminals of myelinated fibers. Top, A collapsed Z-series stack of 0.4 μm confocal sections through a bush baroreceptor terminal shows localization of HCN1 (left) and PGP9.5 (right). PGP9.5 is a ubiquitin hydrolase expressed in neuronal–neuroendocrine cells. Middle, Bush ending is colabeled with HCN2 on the left and the neurofilament mixture on the right. Bottom, HCN4 immunoreactivity on the left is localized to the bush ending identified using the neurofilament mixture (right).
Techniques Used:
