guinea pig anti hcn1  (Alomone Labs)


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    Alomone Labs guinea pig anti hcn1
    Analyses of expression of the Cav2.3 protein in neuronal and nonneuronal cells or in cell membrane of the cells in dorsal root ganglia in vivo. Representative images to demonstrate expression of Cav2.3 in mouse DRG and colabeling with PGP9.5-positive neuronal cells (A), GFAP-positive satellite cells (B) and with <t>HCN1</t> (hyperpolarization-activated cyclic nucleotide-gated) channel in the cell membrane (C). Observed colocalization is highlighted with white arrows. Quantification of coexpression of each neuronal subtype with the Cav2.3 expressing neurons is shown in panel D. Scale bars represent 50 µm in all panels. Tissue samples from 3 independent mice were analyzed.
    Guinea Pig Anti Hcn1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/guinea pig anti hcn1/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    guinea pig anti hcn1 - by Bioz Stars, 2022-01
    90/100 stars

    Images

    1) Product Images from "miR-34c-5p functions as pronociceptive microRNA in cancer pain by targeting Cav2.3 containing calcium channels"

    Article Title: miR-34c-5p functions as pronociceptive microRNA in cancer pain by targeting Cav2.3 containing calcium channels

    Journal: Pain

    doi: 10.1097/j.pain.0000000000000971

    Analyses of expression of the Cav2.3 protein in neuronal and nonneuronal cells or in cell membrane of the cells in dorsal root ganglia in vivo. Representative images to demonstrate expression of Cav2.3 in mouse DRG and colabeling with PGP9.5-positive neuronal cells (A), GFAP-positive satellite cells (B) and with HCN1 (hyperpolarization-activated cyclic nucleotide-gated) channel in the cell membrane (C). Observed colocalization is highlighted with white arrows. Quantification of coexpression of each neuronal subtype with the Cav2.3 expressing neurons is shown in panel D. Scale bars represent 50 µm in all panels. Tissue samples from 3 independent mice were analyzed.
    Figure Legend Snippet: Analyses of expression of the Cav2.3 protein in neuronal and nonneuronal cells or in cell membrane of the cells in dorsal root ganglia in vivo. Representative images to demonstrate expression of Cav2.3 in mouse DRG and colabeling with PGP9.5-positive neuronal cells (A), GFAP-positive satellite cells (B) and with HCN1 (hyperpolarization-activated cyclic nucleotide-gated) channel in the cell membrane (C). Observed colocalization is highlighted with white arrows. Quantification of coexpression of each neuronal subtype with the Cav2.3 expressing neurons is shown in panel D. Scale bars represent 50 µm in all panels. Tissue samples from 3 independent mice were analyzed.

    Techniques Used: Expressing, In Vivo, Mouse Assay

    2) Product Images from "Deletion of the HCN channel auxiliary subunit TRIP8b impairs hippocampal Ih localization and function and promotes antidepressant behavior in mice"

    Article Title: Deletion of the HCN channel auxiliary subunit TRIP8b impairs hippocampal Ih localization and function and promotes antidepressant behavior in mice

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    doi: 10.1523/JNEUROSCI.0936-11.2011

    TRIP8b deletion reduces HCN1 and HCN2 protein levels without affecting HCN1/HCN2 heteromerization, upregulation of the unfolded protein response protein BiP (Grp78), or sequestration of HCN1 protein in Golgi apparatus
    Figure Legend Snippet: TRIP8b deletion reduces HCN1 and HCN2 protein levels without affecting HCN1/HCN2 heteromerization, upregulation of the unfolded protein response protein BiP (Grp78), or sequestration of HCN1 protein in Golgi apparatus

    Techniques Used:

    HCN1 protein density is significantly reduced on the plasma membrane of TRIP8b −/− dendrites
    Figure Legend Snippet: HCN1 protein density is significantly reduced on the plasma membrane of TRIP8b −/− dendrites

    Techniques Used:

    HCN1 is targeted to lysosomes in neurons lacking TRIP8b
    Figure Legend Snippet: HCN1 is targeted to lysosomes in neurons lacking TRIP8b

    Techniques Used:

    HCN1 is localized to multivesicular bodies in distal dendrites of TRIP8b −/− mice
    Figure Legend Snippet: HCN1 is localized to multivesicular bodies in distal dendrites of TRIP8b −/− mice

    Techniques Used: Mouse Assay

    TRIP8b −/− , HCN2 ap/ap and HCN1 −/− mice exhibit reduced depression-like behavior, and TRIP8b −/− mice exhibit impaired motor learning and normal hippocampal-dependent learning and memory
    Figure Legend Snippet: TRIP8b −/− , HCN2 ap/ap and HCN1 −/− mice exhibit reduced depression-like behavior, and TRIP8b −/− mice exhibit impaired motor learning and normal hippocampal-dependent learning and memory

    Techniques Used: Mouse Assay

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    Alomone Labs guinea pig anti hcn1
    Analyses of expression of the Cav2.3 protein in neuronal and nonneuronal cells or in cell membrane of the cells in dorsal root ganglia in vivo. Representative images to demonstrate expression of Cav2.3 in mouse DRG and colabeling with PGP9.5-positive neuronal cells (A), GFAP-positive satellite cells (B) and with <t>HCN1</t> (hyperpolarization-activated cyclic nucleotide-gated) channel in the cell membrane (C). Observed colocalization is highlighted with white arrows. Quantification of coexpression of each neuronal subtype with the Cav2.3 expressing neurons is shown in panel D. Scale bars represent 50 µm in all panels. Tissue samples from 3 independent mice were analyzed.
    Guinea Pig Anti Hcn1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/guinea pig anti hcn1/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    guinea pig anti hcn1 - by Bioz Stars, 2022-01
    90/100 stars
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    Analyses of expression of the Cav2.3 protein in neuronal and nonneuronal cells or in cell membrane of the cells in dorsal root ganglia in vivo. Representative images to demonstrate expression of Cav2.3 in mouse DRG and colabeling with PGP9.5-positive neuronal cells (A), GFAP-positive satellite cells (B) and with HCN1 (hyperpolarization-activated cyclic nucleotide-gated) channel in the cell membrane (C). Observed colocalization is highlighted with white arrows. Quantification of coexpression of each neuronal subtype with the Cav2.3 expressing neurons is shown in panel D. Scale bars represent 50 µm in all panels. Tissue samples from 3 independent mice were analyzed.

    Journal: Pain

    Article Title: miR-34c-5p functions as pronociceptive microRNA in cancer pain by targeting Cav2.3 containing calcium channels

    doi: 10.1097/j.pain.0000000000000971

    Figure Lengend Snippet: Analyses of expression of the Cav2.3 protein in neuronal and nonneuronal cells or in cell membrane of the cells in dorsal root ganglia in vivo. Representative images to demonstrate expression of Cav2.3 in mouse DRG and colabeling with PGP9.5-positive neuronal cells (A), GFAP-positive satellite cells (B) and with HCN1 (hyperpolarization-activated cyclic nucleotide-gated) channel in the cell membrane (C). Observed colocalization is highlighted with white arrows. Quantification of coexpression of each neuronal subtype with the Cav2.3 expressing neurons is shown in panel D. Scale bars represent 50 µm in all panels. Tissue samples from 3 independent mice were analyzed.

    Article Snippet: Primary antibodies used for IF are Guinea pig anti-PGP9.5 (1:100 dilution, 14104, Neuromics, Edina, MN), Guinea pig anti-HCN1 (1:100, Alomone Labs, AGP203) rabbit anti-Cav2.3 antibody (1:80, Alomone Labs, ACC-006), Biotinylated-Isolectin B4 (1:100; B-1205, Vector, Burlingame, CA), Guinea pig Substance P (1:150; Neuromics GP14103), Anti-GFAP (1:500; NeuroMab clone N206A/8, UC Davis, Davis, CA) and Chicken anti-NF200 (1:500; Neuromics CH23015).

    Techniques: Expressing, In Vivo, Mouse Assay

    TRIP8b deletion reduces HCN1 and HCN2 protein levels without affecting HCN1/HCN2 heteromerization, upregulation of the unfolded protein response protein BiP (Grp78), or sequestration of HCN1 protein in Golgi apparatus

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Deletion of the HCN channel auxiliary subunit TRIP8b impairs hippocampal Ih localization and function and promotes antidepressant behavior in mice

    doi: 10.1523/JNEUROSCI.0936-11.2011

    Figure Lengend Snippet: TRIP8b deletion reduces HCN1 and HCN2 protein levels without affecting HCN1/HCN2 heteromerization, upregulation of the unfolded protein response protein BiP (Grp78), or sequestration of HCN1 protein in Golgi apparatus

    Article Snippet: The following primary antibodies were used in these studies: guinea pig (gp) anti-HCN1 , rabbit (rb) anti-HCN1 (against amino acids 778–910 (same as gp anti-HCN1)), gp anti-HCN2 , rb anti-HCN2 (Alomone Labs), mouse (ms) anti-α-tubulin (clone DM1A, Santa Cruz Biotechnology), rb and gp anti-GFP , rb and gp anti-TRIP8b N-terminus ( ; ), rb anti-TRIP8b C-terminus (see above methods and ), gp anti-TRIP8b exon 1a-5, exon 2, and exon 4 (( ) and ), ms anti-MAP2 (clone HM-2, Sigma), ms anti-KDEL (clone 10C3, Stressgen), ms anti-GM130 (clone 35, BD Biosciences), rb anti-Lamp1 (Sigma).

    Techniques:

    HCN1 protein density is significantly reduced on the plasma membrane of TRIP8b −/− dendrites

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Deletion of the HCN channel auxiliary subunit TRIP8b impairs hippocampal Ih localization and function and promotes antidepressant behavior in mice

    doi: 10.1523/JNEUROSCI.0936-11.2011

    Figure Lengend Snippet: HCN1 protein density is significantly reduced on the plasma membrane of TRIP8b −/− dendrites

    Article Snippet: The following primary antibodies were used in these studies: guinea pig (gp) anti-HCN1 , rabbit (rb) anti-HCN1 (against amino acids 778–910 (same as gp anti-HCN1)), gp anti-HCN2 , rb anti-HCN2 (Alomone Labs), mouse (ms) anti-α-tubulin (clone DM1A, Santa Cruz Biotechnology), rb and gp anti-GFP , rb and gp anti-TRIP8b N-terminus ( ; ), rb anti-TRIP8b C-terminus (see above methods and ), gp anti-TRIP8b exon 1a-5, exon 2, and exon 4 (( ) and ), ms anti-MAP2 (clone HM-2, Sigma), ms anti-KDEL (clone 10C3, Stressgen), ms anti-GM130 (clone 35, BD Biosciences), rb anti-Lamp1 (Sigma).

    Techniques:

    HCN1 is targeted to lysosomes in neurons lacking TRIP8b

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Deletion of the HCN channel auxiliary subunit TRIP8b impairs hippocampal Ih localization and function and promotes antidepressant behavior in mice

    doi: 10.1523/JNEUROSCI.0936-11.2011

    Figure Lengend Snippet: HCN1 is targeted to lysosomes in neurons lacking TRIP8b

    Article Snippet: The following primary antibodies were used in these studies: guinea pig (gp) anti-HCN1 , rabbit (rb) anti-HCN1 (against amino acids 778–910 (same as gp anti-HCN1)), gp anti-HCN2 , rb anti-HCN2 (Alomone Labs), mouse (ms) anti-α-tubulin (clone DM1A, Santa Cruz Biotechnology), rb and gp anti-GFP , rb and gp anti-TRIP8b N-terminus ( ; ), rb anti-TRIP8b C-terminus (see above methods and ), gp anti-TRIP8b exon 1a-5, exon 2, and exon 4 (( ) and ), ms anti-MAP2 (clone HM-2, Sigma), ms anti-KDEL (clone 10C3, Stressgen), ms anti-GM130 (clone 35, BD Biosciences), rb anti-Lamp1 (Sigma).

    Techniques:

    HCN1 is localized to multivesicular bodies in distal dendrites of TRIP8b −/− mice

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Deletion of the HCN channel auxiliary subunit TRIP8b impairs hippocampal Ih localization and function and promotes antidepressant behavior in mice

    doi: 10.1523/JNEUROSCI.0936-11.2011

    Figure Lengend Snippet: HCN1 is localized to multivesicular bodies in distal dendrites of TRIP8b −/− mice

    Article Snippet: The following primary antibodies were used in these studies: guinea pig (gp) anti-HCN1 , rabbit (rb) anti-HCN1 (against amino acids 778–910 (same as gp anti-HCN1)), gp anti-HCN2 , rb anti-HCN2 (Alomone Labs), mouse (ms) anti-α-tubulin (clone DM1A, Santa Cruz Biotechnology), rb and gp anti-GFP , rb and gp anti-TRIP8b N-terminus ( ; ), rb anti-TRIP8b C-terminus (see above methods and ), gp anti-TRIP8b exon 1a-5, exon 2, and exon 4 (( ) and ), ms anti-MAP2 (clone HM-2, Sigma), ms anti-KDEL (clone 10C3, Stressgen), ms anti-GM130 (clone 35, BD Biosciences), rb anti-Lamp1 (Sigma).

    Techniques: Mouse Assay

    TRIP8b −/− , HCN2 ap/ap and HCN1 −/− mice exhibit reduced depression-like behavior, and TRIP8b −/− mice exhibit impaired motor learning and normal hippocampal-dependent learning and memory

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Deletion of the HCN channel auxiliary subunit TRIP8b impairs hippocampal Ih localization and function and promotes antidepressant behavior in mice

    doi: 10.1523/JNEUROSCI.0936-11.2011

    Figure Lengend Snippet: TRIP8b −/− , HCN2 ap/ap and HCN1 −/− mice exhibit reduced depression-like behavior, and TRIP8b −/− mice exhibit impaired motor learning and normal hippocampal-dependent learning and memory

    Article Snippet: The following primary antibodies were used in these studies: guinea pig (gp) anti-HCN1 , rabbit (rb) anti-HCN1 (against amino acids 778–910 (same as gp anti-HCN1)), gp anti-HCN2 , rb anti-HCN2 (Alomone Labs), mouse (ms) anti-α-tubulin (clone DM1A, Santa Cruz Biotechnology), rb and gp anti-GFP , rb and gp anti-TRIP8b N-terminus ( ; ), rb anti-TRIP8b C-terminus (see above methods and ), gp anti-TRIP8b exon 1a-5, exon 2, and exon 4 (( ) and ), ms anti-MAP2 (clone HM-2, Sigma), ms anti-KDEL (clone 10C3, Stressgen), ms anti-GM130 (clone 35, BD Biosciences), rb anti-Lamp1 (Sigma).

    Techniques: Mouse Assay