guinea pig anti trpv1 vr1 antibody  (Alomone Labs)


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    Name:
    Guinea pig Anti TRPV1 VR1 Antibody
    Description:
    Guinea pig Anti TRPV1 VR1 Antibody is directed against an epitope of rat TRPV1 channel Guinea pig Anti TRPV1 VR1 Antibody AGP 118 raised in guinea pigs can be used in western blot analysis It has been designed to recognize TRPV1 from mouse rat and human samples The antigen used to immunize guinea pigs is the same as Anti TRPV1 VR1 Antibody ACC 030 raised in rabbit Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies immunoprecipitation etc
    Catalog Number:
    AGP-118
    Price:
    397.0
    Category:
    Primary Antibody
    Applications:
    Immunofluorescence, Immunohistochemistry, Western Blot
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    25 mcl
    Antibody Type:
    Polyclonal Primary Antibodies
    Format:
    Lyophilized Powder
    Host:
    Guinea pig
    Isotype:
    Guinea pig total IgG
    Buy from Supplier


    Structured Review

    Alomone Labs guinea pig anti trpv1 vr1 antibody
    Guinea pig Anti TRPV1 VR1 Antibody
    Guinea pig Anti TRPV1 VR1 Antibody is directed against an epitope of rat TRPV1 channel Guinea pig Anti TRPV1 VR1 Antibody AGP 118 raised in guinea pigs can be used in western blot analysis It has been designed to recognize TRPV1 from mouse rat and human samples The antigen used to immunize guinea pigs is the same as Anti TRPV1 VR1 Antibody ACC 030 raised in rabbit Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies immunoprecipitation etc
    https://www.bioz.com/result/guinea pig anti trpv1 vr1 antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    guinea pig anti trpv1 vr1 antibody - by Bioz Stars, 2021-09
    94/100 stars

    Images

    1) Product Images from "Behavioral and nociceptor states of inflammatory pain across timescales in 2D and 3D"

    Article Title: Behavioral and nociceptor states of inflammatory pain across timescales in 2D and 3D

    Journal: bioRxiv

    doi: 10.1101/2021.06.16.448689

    Hind paw innervating sensory neurons cultured from the inflamed side are more excitable than those from the non-injected side during early inflammation. (A) Schematic representation of retrograde labelling of hind paw innervating sensory neurons with Fast Blue, Inset: Fast Blue positive neuron (blue) following acute dissociation, scale = 50 µm. (B) Relative frequency distributions of dissociated sensory neuron soma diameter from hind paw innervating and total lumbar populations, Insert: Proportion Fast Blue positive cells from acutely dissociated cultures of lumbar DRG. (C) Step-wise current injections were used to determine the rheobase of hind paw innervating sensory neurons from injected (ipsi) and non-injected (contra) sides 4- or 24-hours post-induction of inflammation with carrageenan. (D) Frequency of action potential (AP) discharge following stimulation of sensory neurons with a suprathreshold (2X rheobase). Representative traces from step-wise current injections to determine rheobase of neurons from (E) contralateral and (F) ipsilateral sides 4-hours post injection of carrageenan. (G) A subset of hind paw innervating sensory neurons (blue) express the nociceptive ion channel TRPV1 (magenta), cells positive for both Fast Blue and TRPV1 are identified by yellow pointers, scale = 50 µm. (H) A higher proportion of hind paw innervating neurons expressed TRPV1 after 24-hours of carrageenan-induced inflammation. ** p
    Figure Legend Snippet: Hind paw innervating sensory neurons cultured from the inflamed side are more excitable than those from the non-injected side during early inflammation. (A) Schematic representation of retrograde labelling of hind paw innervating sensory neurons with Fast Blue, Inset: Fast Blue positive neuron (blue) following acute dissociation, scale = 50 µm. (B) Relative frequency distributions of dissociated sensory neuron soma diameter from hind paw innervating and total lumbar populations, Insert: Proportion Fast Blue positive cells from acutely dissociated cultures of lumbar DRG. (C) Step-wise current injections were used to determine the rheobase of hind paw innervating sensory neurons from injected (ipsi) and non-injected (contra) sides 4- or 24-hours post-induction of inflammation with carrageenan. (D) Frequency of action potential (AP) discharge following stimulation of sensory neurons with a suprathreshold (2X rheobase). Representative traces from step-wise current injections to determine rheobase of neurons from (E) contralateral and (F) ipsilateral sides 4-hours post injection of carrageenan. (G) A subset of hind paw innervating sensory neurons (blue) express the nociceptive ion channel TRPV1 (magenta), cells positive for both Fast Blue and TRPV1 are identified by yellow pointers, scale = 50 µm. (H) A higher proportion of hind paw innervating neurons expressed TRPV1 after 24-hours of carrageenan-induced inflammation. ** p

    Techniques Used: Cell Culture, Injection

    Related Articles

    Incubation:

    Article Title: Behavioral and nociceptor states of inflammatory pain across timescales in 2D and 3D
    Article Snippet: .. After washing with PBS containing 0.001% (v/v) Tween-20 (Thermo Fisher Scientific), slides were incubated with antibody dilutant (1 % (w/v) BSA, 5% (v/v) donkey serum and 0.02% (v/v) Triton-X-100 in PBS) at room temperature for 1 hour before overnight incubation at 4 °C with an anti-TRPV1 antibody (1:500; guinea-pig polyclonal; Alomone, AGP-118). ..

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  • 94
    Alomone Labs trpv1 antibody
    <t>TRPV1</t> gene and protein expression using RNA-sequencing data and Western blotting. (A) TRPV1 gene expression in different databases. *P
    Trpv1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trpv1 antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trpv1 antibody - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    94
    Alomone Labs guinea pig anti trpv1 vr1 antibody
    Hind paw innervating sensory neurons cultured from the inflamed side are more excitable than those from the non-injected side during early inflammation. (A) Schematic representation of retrograde labelling of hind paw innervating sensory neurons with Fast Blue, Inset: Fast Blue positive neuron (blue) following acute dissociation, scale = 50 µm. (B) Relative frequency distributions of dissociated sensory neuron soma diameter from hind paw innervating and total lumbar populations, Insert: Proportion Fast Blue positive cells from acutely dissociated cultures of lumbar DRG. (C) Step-wise current injections were used to determine the rheobase of hind paw innervating sensory neurons from injected (ipsi) and non-injected (contra) sides 4- or 24-hours post-induction of inflammation with carrageenan. (D) Frequency of action potential (AP) discharge following stimulation of sensory neurons with a suprathreshold (2X rheobase). Representative traces from step-wise current injections to determine rheobase of neurons from (E) contralateral and (F) ipsilateral sides 4-hours post injection of carrageenan. (G) A subset of hind paw innervating sensory neurons (blue) express the nociceptive ion channel <t>TRPV1</t> (magenta), cells positive for both Fast Blue and TRPV1 are identified by yellow pointers, scale = 50 µm. (H) A higher proportion of hind paw innervating neurons expressed TRPV1 after 24-hours of carrageenan-induced inflammation. ** p
    Guinea Pig Anti Trpv1 Vr1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/guinea pig anti trpv1 vr1 antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    guinea pig anti trpv1 vr1 antibody - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    TRPV1 gene and protein expression using RNA-sequencing data and Western blotting. (A) TRPV1 gene expression in different databases. *P

    Journal: medRxiv

    Article Title: Optimized Flow Cytometric Detection of Transient Receptor Potential Vanilloid-1 (TRPV1) in Human Hematological Malignancies

    doi: 10.1101/2021.08.04.21261521

    Figure Lengend Snippet: TRPV1 gene and protein expression using RNA-sequencing data and Western blotting. (A) TRPV1 gene expression in different databases. *P

    Article Snippet: Exclusion of TRPV1 antibody was used as an additional negative control.

    Techniques: Expressing, RNA Sequencing Assay, Western Blot

    TRPV1 expression using Flow Cytometry. (A) Overview structure of TRPV1 and the 3 antibodies binding sites. Assessment of anti-TRPV1 antibodies: (B) SC-20813, (C) ACC-030, and (D) LS-C150735. Panel D demonstrates clear separation of isotype control from TRPV1 signals in THP-1, U266B1, U937 cells and healthy human lymphocytes using LS-C150735. (E) TRPV1 expression in all patients with hematological malignancies and healthy controls (n=20). TRPV1 in MM, B-NHL and other hematological malignancies group (treated and de novo ) were compared to control. Median fluorescence intensity (MFI) value for individual patients expressed using violin plots with the mean and standard deviation also shown. Patients samples were also expressed as a ratio (MFI patient: MFI control). Overall TRPV1 was significantly higher in all patients with hematological malignancies compared to healthy control (P=0.0351, unpaired t-test) with B-NHL patients having significantly higher TRPV1 compared to control (P= 0.0294, Dunnet’s test)

    Journal: medRxiv

    Article Title: Optimized Flow Cytometric Detection of Transient Receptor Potential Vanilloid-1 (TRPV1) in Human Hematological Malignancies

    doi: 10.1101/2021.08.04.21261521

    Figure Lengend Snippet: TRPV1 expression using Flow Cytometry. (A) Overview structure of TRPV1 and the 3 antibodies binding sites. Assessment of anti-TRPV1 antibodies: (B) SC-20813, (C) ACC-030, and (D) LS-C150735. Panel D demonstrates clear separation of isotype control from TRPV1 signals in THP-1, U266B1, U937 cells and healthy human lymphocytes using LS-C150735. (E) TRPV1 expression in all patients with hematological malignancies and healthy controls (n=20). TRPV1 in MM, B-NHL and other hematological malignancies group (treated and de novo ) were compared to control. Median fluorescence intensity (MFI) value for individual patients expressed using violin plots with the mean and standard deviation also shown. Patients samples were also expressed as a ratio (MFI patient: MFI control). Overall TRPV1 was significantly higher in all patients with hematological malignancies compared to healthy control (P=0.0351, unpaired t-test) with B-NHL patients having significantly higher TRPV1 compared to control (P= 0.0294, Dunnet’s test)

    Article Snippet: Exclusion of TRPV1 antibody was used as an additional negative control.

    Techniques: Expressing, Flow Cytometry, Binding Assay, Fluorescence, Standard Deviation

    Hind paw innervating sensory neurons cultured from the inflamed side are more excitable than those from the non-injected side during early inflammation. (A) Schematic representation of retrograde labelling of hind paw innervating sensory neurons with Fast Blue, Inset: Fast Blue positive neuron (blue) following acute dissociation, scale = 50 µm. (B) Relative frequency distributions of dissociated sensory neuron soma diameter from hind paw innervating and total lumbar populations, Insert: Proportion Fast Blue positive cells from acutely dissociated cultures of lumbar DRG. (C) Step-wise current injections were used to determine the rheobase of hind paw innervating sensory neurons from injected (ipsi) and non-injected (contra) sides 4- or 24-hours post-induction of inflammation with carrageenan. (D) Frequency of action potential (AP) discharge following stimulation of sensory neurons with a suprathreshold (2X rheobase). Representative traces from step-wise current injections to determine rheobase of neurons from (E) contralateral and (F) ipsilateral sides 4-hours post injection of carrageenan. (G) A subset of hind paw innervating sensory neurons (blue) express the nociceptive ion channel TRPV1 (magenta), cells positive for both Fast Blue and TRPV1 are identified by yellow pointers, scale = 50 µm. (H) A higher proportion of hind paw innervating neurons expressed TRPV1 after 24-hours of carrageenan-induced inflammation. ** p

    Journal: bioRxiv

    Article Title: Behavioral and nociceptor states of inflammatory pain across timescales in 2D and 3D

    doi: 10.1101/2021.06.16.448689

    Figure Lengend Snippet: Hind paw innervating sensory neurons cultured from the inflamed side are more excitable than those from the non-injected side during early inflammation. (A) Schematic representation of retrograde labelling of hind paw innervating sensory neurons with Fast Blue, Inset: Fast Blue positive neuron (blue) following acute dissociation, scale = 50 µm. (B) Relative frequency distributions of dissociated sensory neuron soma diameter from hind paw innervating and total lumbar populations, Insert: Proportion Fast Blue positive cells from acutely dissociated cultures of lumbar DRG. (C) Step-wise current injections were used to determine the rheobase of hind paw innervating sensory neurons from injected (ipsi) and non-injected (contra) sides 4- or 24-hours post-induction of inflammation with carrageenan. (D) Frequency of action potential (AP) discharge following stimulation of sensory neurons with a suprathreshold (2X rheobase). Representative traces from step-wise current injections to determine rheobase of neurons from (E) contralateral and (F) ipsilateral sides 4-hours post injection of carrageenan. (G) A subset of hind paw innervating sensory neurons (blue) express the nociceptive ion channel TRPV1 (magenta), cells positive for both Fast Blue and TRPV1 are identified by yellow pointers, scale = 50 µm. (H) A higher proportion of hind paw innervating neurons expressed TRPV1 after 24-hours of carrageenan-induced inflammation. ** p

    Article Snippet: After washing with PBS containing 0.001% (v/v) Tween-20 (Thermo Fisher Scientific), slides were incubated with antibody dilutant (1 % (w/v) BSA, 5% (v/v) donkey serum and 0.02% (v/v) Triton-X-100 in PBS) at room temperature for 1 hour before overnight incubation at 4 °C with an anti-TRPV1 antibody (1:500; guinea-pig polyclonal; Alomone, AGP-118).

    Techniques: Cell Culture, Injection