anti kv7 2  (Alomone Labs)


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    Structured Review

    Alomone Labs anti kv7 2
    Anti Kv7 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti kv7 2/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti kv7 2 - by Bioz Stars, 2021-09
    86/100 stars

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    Incubation:

    Article Title: Neuronal Pentraxin 1 Negatively Regulates Excitatory Synapse Density and Synaptic Plasticity
    Article Snippet: .. Incubation with primary antibodies was performed overnight at 4°C with the following antibodies, diluted in 3% BSA/PBS or 1% NGS/PBS (NP1, VGLUT1, and Kv7.2): mouse monoclonal anti-PSD95 (1:50; Abcam), anti-gephyrin (1:100; Synaptic Systems), anti-NP1 (1:50, BD Transduction), rabbit polyclonal anti-synaptophysin (1:100; Synaptic Systems), anti-Kv7.2 (1:100; Alomone Labs), and guinea pig anti-VGLUT1 (1:200; Millipore Bioscience Research Reagents). ..

    Article Title: Altered Ion Channels in an Animal Model of Charcot-Marie-Tooth Disease Type IA
    Article Snippet: .. Frozen sections and teased fibers were permeabilized by immersion in -20°C acetone for 10 min, blocked at room temperature for 1 h with 5% fish skin gelatin containing 0.1% Triton X-100 in PBS, and incubated overnight at 4°C with various combinations of primary antibodies: rabbit antisera against KCNQ2 [1:200 ( )], Kv3.1b (1:100; Alomone Labs, Jerusalem, Israel), Kv1.2 (1:100; Alomone Labs), Nav1.6 [1:100 ( )], Nav1.8 [1:100 ( )], Nav1.8 [1:500 ( )], ankyrin-G [1:100 ( ; )], contactin [1:100 ( )], ezrin-binding protein 50 kDa (EBP-50) (ab3452; 1:100; Abcam, Cambridge, MA), syndecan-3 [1:300 ( )], or Caspr [1:500 ( )]; and mouse monoclonal antibodies against panNav channels (K58/35; 1:250; Sigma, St. Louis, MO), Nav1.2 [1:100 ( ; )], Nav1.2 (1:50; Upstate Biotechnology, Lake Placid, NY), Caspr [1:50 ( )], annexin II light chain (1:50; BD Transduction Laboratories, San Jose, CA), or myelin-associated glycoprotein (MAG) (clone 513, 1:100; Boehringer Mannheim, Indianapolis, IN); rat monoclonal antibody against E-cadherin (1:50; Sigma); chicken antibody against βIV-spectrin [1:500 ( )]; and a soluble construct of receptor protein tyrosine phosphatase β conjugated to the human Fc fragment (RPTPβ-Fc) [1:3 ( )]. ..

    Article Title: Low expression of Kv7/M channels facilitates intrinsic and network bursting in the developing rat hippocampus
    Article Snippet: .. Membranes were blocked with 5% milk, 0.1% Tween-20 in TBS for 1 h followed by overnight incubation with polyclonal antibodies against the Kv7.2 channel (Alomone labs, Jerusalem, Israel) at 4°C, followed by incubation with appropriate secondary antibodies. ..

    Next-Generation Sequencing:

    Article Title: Neuronal Pentraxin 1 Negatively Regulates Excitatory Synapse Density and Synaptic Plasticity
    Article Snippet: .. Incubation with primary antibodies was performed overnight at 4°C with the following antibodies, diluted in 3% BSA/PBS or 1% NGS/PBS (NP1, VGLUT1, and Kv7.2): mouse monoclonal anti-PSD95 (1:50; Abcam), anti-gephyrin (1:100; Synaptic Systems), anti-NP1 (1:50, BD Transduction), rabbit polyclonal anti-synaptophysin (1:100; Synaptic Systems), anti-Kv7.2 (1:100; Alomone Labs), and guinea pig anti-VGLUT1 (1:200; Millipore Bioscience Research Reagents). ..

    Fluorescence In Situ Hybridization:

    Article Title: Altered Ion Channels in an Animal Model of Charcot-Marie-Tooth Disease Type IA
    Article Snippet: .. Frozen sections and teased fibers were permeabilized by immersion in -20°C acetone for 10 min, blocked at room temperature for 1 h with 5% fish skin gelatin containing 0.1% Triton X-100 in PBS, and incubated overnight at 4°C with various combinations of primary antibodies: rabbit antisera against KCNQ2 [1:200 ( )], Kv3.1b (1:100; Alomone Labs, Jerusalem, Israel), Kv1.2 (1:100; Alomone Labs), Nav1.6 [1:100 ( )], Nav1.8 [1:100 ( )], Nav1.8 [1:500 ( )], ankyrin-G [1:100 ( ; )], contactin [1:100 ( )], ezrin-binding protein 50 kDa (EBP-50) (ab3452; 1:100; Abcam, Cambridge, MA), syndecan-3 [1:300 ( )], or Caspr [1:500 ( )]; and mouse monoclonal antibodies against panNav channels (K58/35; 1:250; Sigma, St. Louis, MO), Nav1.2 [1:100 ( ; )], Nav1.2 (1:50; Upstate Biotechnology, Lake Placid, NY), Caspr [1:50 ( )], annexin II light chain (1:50; BD Transduction Laboratories, San Jose, CA), or myelin-associated glycoprotein (MAG) (clone 513, 1:100; Boehringer Mannheim, Indianapolis, IN); rat monoclonal antibody against E-cadherin (1:50; Sigma); chicken antibody against βIV-spectrin [1:500 ( )]; and a soluble construct of receptor protein tyrosine phosphatase β conjugated to the human Fc fragment (RPTPβ-Fc) [1:3 ( )]. ..

    Construct:

    Article Title: Altered Ion Channels in an Animal Model of Charcot-Marie-Tooth Disease Type IA
    Article Snippet: .. Frozen sections and teased fibers were permeabilized by immersion in -20°C acetone for 10 min, blocked at room temperature for 1 h with 5% fish skin gelatin containing 0.1% Triton X-100 in PBS, and incubated overnight at 4°C with various combinations of primary antibodies: rabbit antisera against KCNQ2 [1:200 ( )], Kv3.1b (1:100; Alomone Labs, Jerusalem, Israel), Kv1.2 (1:100; Alomone Labs), Nav1.6 [1:100 ( )], Nav1.8 [1:100 ( )], Nav1.8 [1:500 ( )], ankyrin-G [1:100 ( ; )], contactin [1:100 ( )], ezrin-binding protein 50 kDa (EBP-50) (ab3452; 1:100; Abcam, Cambridge, MA), syndecan-3 [1:300 ( )], or Caspr [1:500 ( )]; and mouse monoclonal antibodies against panNav channels (K58/35; 1:250; Sigma, St. Louis, MO), Nav1.2 [1:100 ( ; )], Nav1.2 (1:50; Upstate Biotechnology, Lake Placid, NY), Caspr [1:50 ( )], annexin II light chain (1:50; BD Transduction Laboratories, San Jose, CA), or myelin-associated glycoprotein (MAG) (clone 513, 1:100; Boehringer Mannheim, Indianapolis, IN); rat monoclonal antibody against E-cadherin (1:50; Sigma); chicken antibody against βIV-spectrin [1:500 ( )]; and a soluble construct of receptor protein tyrosine phosphatase β conjugated to the human Fc fragment (RPTPβ-Fc) [1:3 ( )]. ..

    Immunocytochemistry:

    Article Title: Protein kinase A stimulates Kv7.1 surface expression by regulating Nedd4-2-dependent endocytic trafficking
    Article Snippet: .. The antibodies used in this study were as follows: goat polyclonal anti-Kv7.1 (immunocytochemistry, 1:100, C-20; Santa Cruz Biotechnology, Heidelberg, Germany), rabbit anti-Kv7.1 (Western blots, 1:1,000, APC-022; Alomone Labs), rabbit anti-Nedd4-2 (Western blot, 1:1,000, no. 4023S; Cell Signaling Technology), rabbit anti-Nedd4-2 (S448) (Western blot, 1:1,000, no. 8063S; Cell Signaling Technology), mouse anti-pCREB (S133) (1:100, 10E9; Merck Millipore), mouse anti-mono- and polyubiquitinylated conjugates (1:250, FK2; Enzo Life Sciences), rabbit anti-calnexin (1:3,000; Enzo Life Sciences), mouse anti-actin (1:15,000, Merck Millipore), and mouse anti-LAMP2 (1:100, AC17; Acris antibodies, Herford, Germany). ..

    Western Blot:

    Article Title: Protein kinase A stimulates Kv7.1 surface expression by regulating Nedd4-2-dependent endocytic trafficking
    Article Snippet: .. The antibodies used in this study were as follows: goat polyclonal anti-Kv7.1 (immunocytochemistry, 1:100, C-20; Santa Cruz Biotechnology, Heidelberg, Germany), rabbit anti-Kv7.1 (Western blots, 1:1,000, APC-022; Alomone Labs), rabbit anti-Nedd4-2 (Western blot, 1:1,000, no. 4023S; Cell Signaling Technology), rabbit anti-Nedd4-2 (S448) (Western blot, 1:1,000, no. 8063S; Cell Signaling Technology), mouse anti-pCREB (S133) (1:100, 10E9; Merck Millipore), mouse anti-mono- and polyubiquitinylated conjugates (1:250, FK2; Enzo Life Sciences), rabbit anti-calnexin (1:3,000; Enzo Life Sciences), mouse anti-actin (1:15,000, Merck Millipore), and mouse anti-LAMP2 (1:100, AC17; Acris antibodies, Herford, Germany). ..

    SDS Page:

    Article Title: Neuronal Pentraxin 1 Negatively Regulates Excitatory Synapse Density and Synaptic Plasticity
    Article Snippet: .. The bound biotinylated proteins were eluted by incubating for 1 h at room temperature with SDS-PAGE buffer (62.5 m m Tris-HCl, pH 6.8, 1% SDS, 10% glycerol, and 50 m m DTT) followed by centrifugation for 2 min at 1000 × g. Biotinylated proteins were separated in 8% SDS-PAGE and transferred to PVDF membranes (Millipore), which were probed with one of the following antibodies: rabbit anti-Kv7.2 antibody (1:2000; Alomone Labs), rabbit anti-GluA1 antibody (1:1000; Millipore), mouse monoclonal anti-GluA2 antibody (1:1000; Neuromab), and mouse monoclonal anti-GluN1 (1:2000; Becton Dickinson) as described above. ..

    Centrifugation:

    Article Title: Neuronal Pentraxin 1 Negatively Regulates Excitatory Synapse Density and Synaptic Plasticity
    Article Snippet: .. The bound biotinylated proteins were eluted by incubating for 1 h at room temperature with SDS-PAGE buffer (62.5 m m Tris-HCl, pH 6.8, 1% SDS, 10% glycerol, and 50 m m DTT) followed by centrifugation for 2 min at 1000 × g. Biotinylated proteins were separated in 8% SDS-PAGE and transferred to PVDF membranes (Millipore), which were probed with one of the following antibodies: rabbit anti-Kv7.2 antibody (1:2000; Alomone Labs), rabbit anti-GluA1 antibody (1:1000; Millipore), mouse monoclonal anti-GluA2 antibody (1:1000; Neuromab), and mouse monoclonal anti-GluN1 (1:2000; Becton Dickinson) as described above. ..

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  • 86
    Alomone Labs anti kv7 2
    Anti Kv7 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti kv7 2/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti kv7 2 - by Bioz Stars, 2021-09
    86/100 stars
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