anti hcn4 agp 004  (Alomone Labs)

Journal: International Journal of Molecular Sciences

Article Title: Structural Identification of the Pacemaker Cells and Expression of Hyperpolarization-Activated Cyclic Nucleotide-Gated (HCN) Channels in the Heart of the Wild Atlantic Cod, Gadus morhua (Linnaeus, 1758)

doi: 10.3390/ijms22147539

Figure Lengend Snippet: Double immunolabeling with antibodies against Hu (( a ), green signal and arrows) and HCN4 (( b ), red signal and arrows) in the SAR. HCN4 was weakly expressed in the pacemaker cells (green, red, and white arrows) that appeared to be innervated by Hu-immunoreactive axons and nerve fibers (arrowheads). Areas of pacemaker tissue with abundant pacemaker cells are boxed in ( c ). Scale bars: 20 µm.

Article Snippet: HCN4 antibodies as reliable markers of the pacemaker cells as well as the controls of HCN4 and Islet-1 antibodies in the heart of zebrafish were reported by [ , ].

Techniques: Immunolabeling

Journal: International Journal of Molecular Sciences

Article Title: Structural Identification of the Pacemaker Cells and Expression of Hyperpolarization-Activated Cyclic Nucleotide-Gated (HCN) Channels in the Heart of the Wild Atlantic Cod, Gadus morhua (Linnaeus, 1758)

doi: 10.3390/ijms22147539

Figure Lengend Snippet: Relative expression levels of hcn1 , hcn2a , hcn2b and hcn4 in sinus, atrium, ventricle and bulbus arteriosus in Atlantic cod. Transcripts were quantified by qPCR, normalized using the geometric average of ubi and eef1 expression and shown as relative values compared to hcn1 transcript levels in each sample. Data are expressed in arbitrary units (A.U.) as mean ± S.E. ( n = 5). Different superscript letters ( a, b ) indicate significant differences in transcript levels between hcn paralogues in each heart region. Differences in hcn transcript levels within each heart area were determined by one-way ANOVA with a Holm–Sidak post hoc test ( p

Article Snippet: HCN4 antibodies as reliable markers of the pacemaker cells as well as the controls of HCN4 and Islet-1 antibodies in the heart of zebrafish were reported by [ , ].

Techniques: Expressing, Real-time Polymerase Chain Reaction

Journal: Cells

Article Title: L-Type Cav1.3 Calcium Channels Are Required for Beta-Adrenergic Triggered Automaticity in Dormant Mouse Sinoatrial Pacemaker Cells

doi: 10.3390/cells11071114

Figure Lengend Snippet: Co-labeling of Ca v 1.3 and HCN4 in mouse SAN. ( A ) Immunolabeling of Ca v 1.3 (red) and HCN4 (green) in the isolated cranial SAN-RA preparation. ( B ) Left panels: close-up views of the area circled in ( A ); right panels: enlargement with cells expressing Ca v 1.3 or HCN4, from boxed region in left panel. ( C ) Immunolabeling of regions co-expressing Ca v 1.3 and HCN4. The left panel shows the region in accordance with the posterior nodal extension (PNL). The yellow rectangle identifies the region considered for close-up views shown in the left panels. Left panels, from top to bottom, indicate corresponding Ca v 1.3 and HCN4 immunostaining. Yellow arrows indicate SANC co-expressing Ca v 1.3 and HCN4.

Article Snippet: SAN was incubated with anti-Cav1.3 (rabbit, 1:200, obtained from [ ] and anti-HCN4 (guinea pig, 1:200, Alomone labs, Cat. AGP-004) primary antibodies at 4 °C overnight.

Techniques: Labeling, Immunolabeling, Isolation, Expressing, Immunostaining

Journal: Cells

Article Title: L-Type Cav1.3 Calcium Channels Are Required for Beta-Adrenergic Triggered Automaticity in Dormant Mouse Sinoatrial Pacemaker Cells

doi: 10.3390/cells11071114

Figure Lengend Snippet: Expression of Ca v 1.3 and HCN4 in mouse SAN. ( A ) Immunolabeling of Ca v 1.3 (green) and HCN4 (red) in n = 64 isolated SANC with distribution of SANC based on Ca v 1.3 or HCN4 fluorescence intensity. Low fluorescence intensity is related to a lower expression of the channels while high density is related to a higher expression. ( B ) Bright-field image of a SAN-RA (N = 3) preparation used for immunolabeling of Ca v 1.3. ( C ) Immunolabeling of Ca v 1.3 (red) of the preparation in ( B ). ( D ) Immunolabeling of Ca v 1.3 in the enlarged region corresponding to the white circle in ( C ). ( E ) Close-up view of Ca v 1.3-expressing cells (red), together with nuclear labeling of DNA (blue), in the field corresponding to the central square in panel ( D ). The yellow arrow indicates Ca v 1.3 immunoreactive cells with punctate staining. Abbreviations: RA, right atrium; SVC, superior vena cava ; IVC, inferior vena cava ; IAS, inter-atrial septum; CS, coronary sinus (cut-open view); CT, crista terminalis . The white dashed line roughly indicates the CT boundary.

Article Snippet: SAN was incubated with anti-Cav1.3 (rabbit, 1:200, obtained from [ ] and anti-HCN4 (guinea pig, 1:200, Alomone labs, Cat. AGP-004) primary antibodies at 4 °C overnight.

Techniques: Expressing, Immunolabeling, Isolation, Fluorescence, Labeling, Staining