Journal: Cells
Article Title: L-Type Cav1.3 Calcium Channels Are Required for Beta-Adrenergic Triggered Automaticity in Dormant Mouse Sinoatrial Pacemaker Cells
doi: 10.3390/cells11071114
Figure Lengend Snippet: Expression of Ca v 1.3 and HCN4 in mouse SAN. ( A ) Immunolabeling of Ca v 1.3 (green) and HCN4 (red) in n = 64 isolated SANC with distribution of SANC based on Ca v 1.3 or HCN4 fluorescence intensity. Low fluorescence intensity is related to a lower expression of the channels while high density is related to a higher expression. ( B ) Bright-field image of a SAN-RA (N = 3) preparation used for immunolabeling of Ca v 1.3. ( C ) Immunolabeling of Ca v 1.3 (red) of the preparation in ( B ). ( D ) Immunolabeling of Ca v 1.3 in the enlarged region corresponding to the white circle in ( C ). ( E ) Close-up view of Ca v 1.3-expressing cells (red), together with nuclear labeling of DNA (blue), in the field corresponding to the central square in panel ( D ). The yellow arrow indicates Ca v 1.3 immunoreactive cells with punctate staining. Abbreviations: RA, right atrium; SVC, superior vena cava ; IVC, inferior vena cava ; IAS, inter-atrial septum; CS, coronary sinus (cut-open view); CT, crista terminalis . The white dashed line roughly indicates the CT boundary.
Article Snippet: SAN was incubated with anti-Cav1.3 (rabbit, 1:200, obtained from [ ] and anti-HCN4 (guinea pig, 1:200, Alomone labs, Cat. AGP-004) primary antibodies at 4 °C overnight.
Techniques: Expressing, Immunolabeling, Isolation, Fluorescence, Labeling, Staining