Journal: International Journal of Molecular Sciences
Article Title: Structural Identification of the Pacemaker Cells and Expression of Hyperpolarization-Activated Cyclic Nucleotide-Gated (HCN) Channels in the Heart of the Wild Atlantic Cod, Gadus morhua (Linnaeus, 1758)
Figure Lengend Snippet: Relative expression levels of hcn1 , hcn2a , hcn2b and hcn4 in sinus, atrium, ventricle and bulbus arteriosus in Atlantic cod. Transcripts were quantified by qPCR, normalized using the geometric average of ubi and eef1 expression and shown as relative values compared to hcn1 transcript levels in each sample. Data are expressed in arbitrary units (A.U.) as mean ± S.E. ( n = 5). Different superscript letters ( a, b ) indicate significant differences in transcript levels between hcn paralogues in each heart region. Differences in hcn transcript levels within each heart area were determined by one-way ANOVA with a Holm–Sidak post hoc test ( p
Article Snippet: HCN4 antibodies as reliable markers of the pacemaker cells as well as the controls of HCN4 and Islet-1 antibodies in the heart of zebrafish were reported by [ , ].
Techniques: Expressing, Real-time Polymerase Chain Reaction