anti slo1  (Alomone Labs)

Journal: FEBS letters

Article Title: The ?1-subunit of Na+/K+-ATPase interacts with BKCa channels and affects their steady-state expression on the cell surface

doi: 10.1016/j.febslet.2009.08.039

Figure Lengend Snippet: NKβ1 down-regulation reduces the cell surface expression of Slo1 channels in HEK293T cells. A, Cell-surface biotinylation assay showing cell surface and total expression of Myc-Slo1 in HEK293T cell transiently cotransfected with si-NKβ1

Article Snippet: Signals from anti-Slo1 and anti-NKβ1 show extensive overlap, especially in intracellular compartments of somatic areas.

Techniques: Expressing, Cell Surface Biotinylation Assay

Journal: FEBS letters

Article Title: The ?1-subunit of Na+/K+-ATPase interacts with BKCa channels and affects their steady-state expression on the cell surface

doi: 10.1016/j.febslet.2009.08.039

Figure Lengend Snippet: NKβ1 interacts with neuronal Slo1 channels. A, B Schematic representations of Slo1 and NKβ1 respectively. The bait fragment used in the yeast two-hybrid screen is highlighted in blue. C, Co-immunoprecipitation of NKβ1 and Slo1

Article Snippet: Signals from anti-Slo1 and anti-NKβ1 show extensive overlap, especially in intracellular compartments of somatic areas.

Techniques: Two Hybrid Screening, Immunoprecipitation

Journal: FEBS letters

Article Title: The ?1-subunit of Na+/K+-ATPase interacts with BKCa channels and affects their steady-state expression on the cell surface

doi: 10.1016/j.febslet.2009.08.039

Figure Lengend Snippet: Interaction between NKβ1 and Slo1 occurs at multiple regions. A, GST pull-down assay carried out on chick CG lysates showing that a fusion protein containing the bait, GST-Slo1(G785-A985) binds to NKβ1. Additional fusion proteins from

Article Snippet: Signals from anti-Slo1 and anti-NKβ1 show extensive overlap, especially in intracellular compartments of somatic areas.

Techniques: Pull Down Assay

Journal: FEBS letters

Article Title: The ?1-subunit of Na+/K+-ATPase interacts with BKCa channels and affects their steady-state expression on the cell surface

doi: 10.1016/j.febslet.2009.08.039

Figure Lengend Snippet: Co-localization pattern of NKβ1 and Slo1. Top panel shows co-localization of NKβ1 (red) and Slo1 (green) in E9 chick CG neurons. The regions enclosed by white squares are magnified in the bottom panels to show neurites and cell bodies.

Article Snippet: Signals from anti-Slo1 and anti-NKβ1 show extensive overlap, especially in intracellular compartments of somatic areas.

Techniques:

Journal: FEBS letters

Article Title: The ?1-subunit of Na+/K+-ATPase interacts with BKCa channels and affects their steady-state expression on the cell surface

doi: 10.1016/j.febslet.2009.08.039

Figure Lengend Snippet: Inside-out patch recordings made from HEK293T cells expressing Slo1. A, Typical families of currents evoked by voltage steps (-80 mV to +80 mV) in inside-out patches from transfected HEK293T cells. The bath solution contained 20 μM Ca 2+ . No currents

Article Snippet: Signals from anti-Slo1 and anti-NKβ1 show extensive overlap, especially in intracellular compartments of somatic areas.

Techniques: Expressing, Transfection

Journal: American Journal of Physiology - Renal Physiology

Article Title: Regulation of podocyte BKCa channels by synaptopodin, Rho, and actin microfilaments

doi: 10.1152/ajprenal.00206.2010

Figure Lengend Snippet: Coexpression of Synpo increases macroscopic currents through BK Ca channels in HEK293T cells transiently expressing Slo1. A : representative traces showing families of whole cell currents evoked by depolarizing steps as indicated above the current traces.

Article Snippet: Also, Synpo was detected in immunoprecipitates prepared with Alomone anti-Slo1 or the custom-made anti-Slo1 ( ).

Techniques: Expressing

Journal: American Journal of Physiology - Renal Physiology

Article Title: Regulation of podocyte BKCa channels by synaptopodin, Rho, and actin microfilaments

doi: 10.1152/ajprenal.00206.2010

Figure Lengend Snippet: Colocalization and interaction of Slo1 and Synpo in mouse glomeruli. A : from a paraffin section of mouse kidney cortex, confocal image of double-label immunofluorescence showing Slo1 (red), Synpo (green), and a merged image, as indicated. Note colocalization

Article Snippet: Also, Synpo was detected in immunoprecipitates prepared with Alomone anti-Slo1 or the custom-made anti-Slo1 ( ).

Techniques: Paraffin Section, Immunofluorescence

Journal: American Journal of Physiology - Renal Physiology

Article Title: Regulation of podocyte BKCa channels by synaptopodin, Rho, and actin microfilaments

doi: 10.1152/ajprenal.00206.2010

Figure Lengend Snippet: Synpo knockdown reduces cell surface expression of podocyte BK Ca channels. A : representative cell surface biotinylation assay showing marked reduction in surface Slo1 in Synpo KD cells. Con, control. B : densitometric quantification of 3 replications of

Article Snippet: Also, Synpo was detected in immunoprecipitates prepared with Alomone anti-Slo1 or the custom-made anti-Slo1 ( ).

Techniques: Expressing, Cell Surface Biotinylation Assay

Journal: American Journal of Physiology - Renal Physiology

Article Title: Regulation of podocyte BKCa channels by synaptopodin, Rho, and actin microfilaments

doi: 10.1152/ajprenal.00206.2010

Figure Lengend Snippet: Interaction between pore-forming subunit of large-conductance Ca 2+ -activated K + (BK Ca ) channels (Slo1) and synaptopodin (Synpo). A : representative example of reciprocal coimmunoprecipitation of Slo1 and Synpo transiently expressed in HEK293T cells. Immunoprecipitation

Article Snippet: Also, Synpo was detected in immunoprecipitates prepared with Alomone anti-Slo1 or the custom-made anti-Slo1 ( ).

Techniques: Immunoprecipitation

Journal: American Journal of Physiology - Renal Physiology

Article Title: Regulation of podocyte BKCa channels by synaptopodin, Rho, and actin microfilaments

doi: 10.1152/ajprenal.00206.2010

Figure Lengend Snippet: Interactions between Slo1 and Synpo mapped with glutathione S -transferase (GST) pull-down assays. A : Synpo was pulled out of podocyte lysates (arrow) with a GST fusion protein comprised of the most distal portion of the large cytosolic COOH terminal of

Article Snippet: Also, Synpo was detected in immunoprecipitates prepared with Alomone anti-Slo1 or the custom-made anti-Slo1 ( ).

Techniques:

Journal: American Journal of Physiology - Renal Physiology

Article Title: Regulation of podocyte BKCa channels by synaptopodin, Rho, and actin microfilaments

doi: 10.1152/ajprenal.00206.2010

Figure Lengend Snippet: Coexpression of Synpo increases steady-state surface expression of Slo1 in HEK293T cells. A : representative cell surface biotinylation assay in HEK293T cells transiently expressing Myc-Slo1 and/or Flag-Synpo as indicated. Stimulatory effect of Synpo was

Article Snippet: Also, Synpo was detected in immunoprecipitates prepared with Alomone anti-Slo1 or the custom-made anti-Slo1 ( ).

Techniques: Expressing, Cell Surface Biotinylation Assay

Journal: American Journal of Physiology - Renal Physiology

Article Title: Regulation of podocyte BKCa channels by synaptopodin, Rho, and actin microfilaments

doi: 10.1152/ajprenal.00206.2010

Figure Lengend Snippet: Synpo and Slo1 distribution in control and Synpo KD podocyte cell lines. A : in control cells, there is close colocalization between Slo1 and Synpo in intracellular compartments and around the periphery of the cells, especially in regions of membrane ruffling.

Article Snippet: Also, Synpo was detected in immunoprecipitates prepared with Alomone anti-Slo1 or the custom-made anti-Slo1 ( ).

Techniques: