hcn4 channel  (Alomone Labs)


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    Name:
    Guinea pig Anti HCN4 Antibody
    Description:
    Guinea pig Anti HCN4 Antibody AGP 004 raised in guinea pigs is a highly specific antibody directed against an epitope of the human protein The antibody can be used in western blot and immunohistochemistry applications It has been designed to recognize HCN4 from human rat and mouse samples The antigen used to immunize guinea pigs is the same as Anti HCN4 Antibody APC 052 raised in rabbit Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies immunoprecipitation etc
    Catalog Number:
    AGP-004
    Price:
    397.0
    Category:
    Primary Antibody
    Applications:
    Immunofluorescence, Immunohistochemistry, Western Blot
    Purity:
    The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and then the IgG fraction was purified on immobilized antigen.
    Immunogen:
    GST fusion protein
    Size:
    25 mcl
    Antibody Type:
    Polyclonal Primary Antibodies
    Format:
    Lyophilized Powder
    Host:
    Guinea pig
    Isotype:
    Guinea pig total IgG
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    Structured Review

    Alomone Labs hcn4 channel
    Guinea pig Anti HCN4 Antibody
    Guinea pig Anti HCN4 Antibody AGP 004 raised in guinea pigs is a highly specific antibody directed against an epitope of the human protein The antibody can be used in western blot and immunohistochemistry applications It has been designed to recognize HCN4 from human rat and mouse samples The antigen used to immunize guinea pigs is the same as Anti HCN4 Antibody APC 052 raised in rabbit Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies immunoprecipitation etc
    https://www.bioz.com/result/hcn4 channel/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hcn4 channel - by Bioz Stars, 2021-09
    92/100 stars

    Images

    1) Product Images from "The Ionotropic P2X4 Receptor has Unique Properties in the Heart by Mediating the Negative Chronotropic Effect of ATP While Increasing the Ventricular Inotropy"

    Article Title: The Ionotropic P2X4 Receptor has Unique Properties in the Heart by Mediating the Negative Chronotropic Effect of ATP While Increasing the Ventricular Inotropy

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2019.01103

    Representative confocal micrographs showing the immunolocalization of the P2X4 receptor (Apr-002, C-terminus, Alomone) and NCX1 (Anx-011, Alomone) protein in the sinoatrial node (SAN), right atria (RA) and right ventricle (RV). The SAN was identified based on its low Cx43 (green) and high HCN4 (magenta) protein expression (left hand-side images). Images were taken from whole-mount heart preparations including the three analyzed regions, SAN, RA and RV. Dashed lines represent boundaries of the SAN. The pulmonary parenchyma was used as a structural support to facilitate immunostaining of myocardial sections and it is visible in the bottom right quadrant of each SAN image. White arrows indicate blood vessels including the SAN artery. Scale bar 30 µm. Images are representative of three different individuals.
    Figure Legend Snippet: Representative confocal micrographs showing the immunolocalization of the P2X4 receptor (Apr-002, C-terminus, Alomone) and NCX1 (Anx-011, Alomone) protein in the sinoatrial node (SAN), right atria (RA) and right ventricle (RV). The SAN was identified based on its low Cx43 (green) and high HCN4 (magenta) protein expression (left hand-side images). Images were taken from whole-mount heart preparations including the three analyzed regions, SAN, RA and RV. Dashed lines represent boundaries of the SAN. The pulmonary parenchyma was used as a structural support to facilitate immunostaining of myocardial sections and it is visible in the bottom right quadrant of each SAN image. White arrows indicate blood vessels including the SAN artery. Scale bar 30 µm. Images are representative of three different individuals.

    Techniques Used: Expressing, Immunostaining

    Related Articles

    Staining:

    Article Title: Using Gjd3-CreEGFP mice to examine atrioventricular node morphology and composition
    Article Snippet: .. Antibodies The following primary antibodies were used [species, target, dilution, company and/or product number]: rabbit anti-Cx40, 1:250 (Alpha Diagnostic International); rabbit polyclonal anti-Cx30.2 (LifeTechnologies, #40-7400), 4 µg/ml for IF staining and 1 µg/ml for WB analysis; mouse mAb α-Tubulin (DM1A) (Cell-Signaling Technology, #3873), 1:1000 for WB analysis; guinea-pig polyclonal anti-Hcn4 (Alomone Labs, #AGP-004), 1:200 for IF; rabbit polyclonal anti-Hcn4, 1:200 for WB analysis, (Alomone Labs, #APC-052); rabbit polyclonal anti-Tbx3 (abcam, #ab99302), 5 µg/ml for IF staining; Pecam1/CD31 Rat anti-Mouse, Unlabeled, Clone: MEC 13.3 (BD Biosciences, #BDB553370), 1:50; rabbit polyclonal anti-Nppa (Millipore, #AB5490), 1:100; mouse monoclonal anti-sarcomeric actinin (Sigma, #A7811), 1:100; rabbit polyclonal anti- Myl2 antibody, 1:100 (ProteinTech); and chicken polyclonal anti-GFP antibody, 1:100 (Thermo Fisher Scientific, #A10262). ..

    Western Blot:

    Article Title: Using Gjd3-CreEGFP mice to examine atrioventricular node morphology and composition
    Article Snippet: .. Antibodies The following primary antibodies were used [species, target, dilution, company and/or product number]: rabbit anti-Cx40, 1:250 (Alpha Diagnostic International); rabbit polyclonal anti-Cx30.2 (LifeTechnologies, #40-7400), 4 µg/ml for IF staining and 1 µg/ml for WB analysis; mouse mAb α-Tubulin (DM1A) (Cell-Signaling Technology, #3873), 1:1000 for WB analysis; guinea-pig polyclonal anti-Hcn4 (Alomone Labs, #AGP-004), 1:200 for IF; rabbit polyclonal anti-Hcn4, 1:200 for WB analysis, (Alomone Labs, #APC-052); rabbit polyclonal anti-Tbx3 (abcam, #ab99302), 5 µg/ml for IF staining; Pecam1/CD31 Rat anti-Mouse, Unlabeled, Clone: MEC 13.3 (BD Biosciences, #BDB553370), 1:50; rabbit polyclonal anti-Nppa (Millipore, #AB5490), 1:100; mouse monoclonal anti-sarcomeric actinin (Sigma, #A7811), 1:100; rabbit polyclonal anti- Myl2 antibody, 1:100 (ProteinTech); and chicken polyclonal anti-GFP antibody, 1:100 (Thermo Fisher Scientific, #A10262). ..

    Incubation:

    Article Title: Hyperpolarization-Activated Cyclic Nucleotide-Gated Ion (HCN) Channels Regulate PC12 Cell Differentiation Toward Sympathetic Neuron
    Article Snippet: .. After washing six times with PBS (5 min for each wash), cells were permeated by incubation in 0.5% Triton X-100 in PBS for 5 min, rinsed six times with PBS, blocked with 10% sheep serum in PBS for 45 min, washed once with 1% sheep serum in PBS, and incubated in a refrigerator (4°C) overnight in the presence or absence of the primary antibodies diluted with 1% sheep serum in PBS: 1:50 dilution for rabbit anti-HCN1 (APC-056), anti-HCN2 (APC-030), anti-HCN3 (APC-057), anti-HCN4 (AGP-004) (Alomone Labs, Jerusalem, Israel), anti-GAP43 (sc-135915) and anti-TH (sc-136100) (Santa Cruz Biotechnology, Inc., United States). ..

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  • 92
    Alomone Labs hcn4 channel
    Representative confocal micrographs showing the immunolocalization of the P2X4 receptor (Apr-002, C-terminus, Alomone) and NCX1 (Anx-011, Alomone) protein in the sinoatrial node (SAN), right atria (RA) and right ventricle (RV). The SAN was identified based on its low Cx43 (green) and high <t>HCN4</t> (magenta) protein expression (left hand-side images). Images were taken from whole-mount heart preparations including the three analyzed regions, SAN, RA and RV. Dashed lines represent boundaries of the SAN. The pulmonary parenchyma was used as a structural support to facilitate immunostaining of myocardial sections and it is visible in the bottom right quadrant of each SAN image. White arrows indicate blood vessels including the SAN artery. Scale bar 30 µm. Images are representative of three different individuals.
    Hcn4 Channel, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcn4 channel/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hcn4 channel - by Bioz Stars, 2021-09
    92/100 stars
      Buy from Supplier

    95
    Alomone Labs hcn4 antibodies
    Double immunolabeling with antibodies against Hu (( a ), green signal and arrows) and <t>HCN4</t> (( b ), red signal and arrows) in the SAR. HCN4 was weakly expressed in the pacemaker cells (green, red, and white arrows) that appeared to be innervated by Hu-immunoreactive axons and nerve fibers (arrowheads). Areas of pacemaker tissue with abundant pacemaker cells are boxed in ( c ). Scale bars: 20 µm.
    Hcn4 Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcn4 antibodies/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hcn4 antibodies - by Bioz Stars, 2021-09
    95/100 stars
      Buy from Supplier

    Image Search Results


    Representative confocal micrographs showing the immunolocalization of the P2X4 receptor (Apr-002, C-terminus, Alomone) and NCX1 (Anx-011, Alomone) protein in the sinoatrial node (SAN), right atria (RA) and right ventricle (RV). The SAN was identified based on its low Cx43 (green) and high HCN4 (magenta) protein expression (left hand-side images). Images were taken from whole-mount heart preparations including the three analyzed regions, SAN, RA and RV. Dashed lines represent boundaries of the SAN. The pulmonary parenchyma was used as a structural support to facilitate immunostaining of myocardial sections and it is visible in the bottom right quadrant of each SAN image. White arrows indicate blood vessels including the SAN artery. Scale bar 30 µm. Images are representative of three different individuals.

    Journal: Frontiers in Pharmacology

    Article Title: The Ionotropic P2X4 Receptor has Unique Properties in the Heart by Mediating the Negative Chronotropic Effect of ATP While Increasing the Ventricular Inotropy

    doi: 10.3389/fphar.2019.01103

    Figure Lengend Snippet: Representative confocal micrographs showing the immunolocalization of the P2X4 receptor (Apr-002, C-terminus, Alomone) and NCX1 (Anx-011, Alomone) protein in the sinoatrial node (SAN), right atria (RA) and right ventricle (RV). The SAN was identified based on its low Cx43 (green) and high HCN4 (magenta) protein expression (left hand-side images). Images were taken from whole-mount heart preparations including the three analyzed regions, SAN, RA and RV. Dashed lines represent boundaries of the SAN. The pulmonary parenchyma was used as a structural support to facilitate immunostaining of myocardial sections and it is visible in the bottom right quadrant of each SAN image. White arrows indicate blood vessels including the SAN artery. Scale bar 30 µm. Images are representative of three different individuals.

    Article Snippet: Representative confocal micrographs showing the immunolocalization of the P2X4 receptor (Apr-024, extracellular loop, Alomone) and of the HCN4 channel (Agp-004, Alomone) in the rat sinoatrial node (SAN); images obtained in right atria (RA) and right ventricle (RV) are also shown for comparison.

    Techniques: Expressing, Immunostaining

    Double immunolabeling with antibodies against Hu (( a ), green signal and arrows) and HCN4 (( b ), red signal and arrows) in the SAR. HCN4 was weakly expressed in the pacemaker cells (green, red, and white arrows) that appeared to be innervated by Hu-immunoreactive axons and nerve fibers (arrowheads). Areas of pacemaker tissue with abundant pacemaker cells are boxed in ( c ). Scale bars: 20 µm.

    Journal: International Journal of Molecular Sciences

    Article Title: Structural Identification of the Pacemaker Cells and Expression of Hyperpolarization-Activated Cyclic Nucleotide-Gated (HCN) Channels in the Heart of the Wild Atlantic Cod, Gadus morhua (Linnaeus, 1758)

    doi: 10.3390/ijms22147539

    Figure Lengend Snippet: Double immunolabeling with antibodies against Hu (( a ), green signal and arrows) and HCN4 (( b ), red signal and arrows) in the SAR. HCN4 was weakly expressed in the pacemaker cells (green, red, and white arrows) that appeared to be innervated by Hu-immunoreactive axons and nerve fibers (arrowheads). Areas of pacemaker tissue with abundant pacemaker cells are boxed in ( c ). Scale bars: 20 µm.

    Article Snippet: HCN4 antibodies as reliable markers of the pacemaker cells as well as the controls of HCN4 and Islet-1 antibodies in the heart of zebrafish were reported by [ , ].

    Techniques: Immunolabeling

    Relative expression levels of hcn1 , hcn2a , hcn2b and hcn4 in sinus, atrium, ventricle and bulbus arteriosus in Atlantic cod. Transcripts were quantified by qPCR, normalized using the geometric average of ubi and eef1 expression and shown as relative values compared to hcn1 transcript levels in each sample. Data are expressed in arbitrary units (A.U.) as mean ± S.E. ( n = 5). Different superscript letters ( a, b ) indicate significant differences in transcript levels between hcn paralogues in each heart region. Differences in hcn transcript levels within each heart area were determined by one-way ANOVA with a Holm–Sidak post hoc test ( p

    Journal: International Journal of Molecular Sciences

    Article Title: Structural Identification of the Pacemaker Cells and Expression of Hyperpolarization-Activated Cyclic Nucleotide-Gated (HCN) Channels in the Heart of the Wild Atlantic Cod, Gadus morhua (Linnaeus, 1758)

    doi: 10.3390/ijms22147539

    Figure Lengend Snippet: Relative expression levels of hcn1 , hcn2a , hcn2b and hcn4 in sinus, atrium, ventricle and bulbus arteriosus in Atlantic cod. Transcripts were quantified by qPCR, normalized using the geometric average of ubi and eef1 expression and shown as relative values compared to hcn1 transcript levels in each sample. Data are expressed in arbitrary units (A.U.) as mean ± S.E. ( n = 5). Different superscript letters ( a, b ) indicate significant differences in transcript levels between hcn paralogues in each heart region. Differences in hcn transcript levels within each heart area were determined by one-way ANOVA with a Holm–Sidak post hoc test ( p

    Article Snippet: HCN4 antibodies as reliable markers of the pacemaker cells as well as the controls of HCN4 and Islet-1 antibodies in the heart of zebrafish were reported by [ , ].

    Techniques: Expressing, Real-time Polymerase Chain Reaction