anti hcn4 agp 004  (Alomone Labs)


Bioz Verified Symbol Alomone Labs is a verified supplier
Bioz Manufacturer Symbol Alomone Labs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Alomone Labs anti hcn4 agp 004
    Anti Hcn4 Agp 004, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti hcn4 agp 004/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti hcn4 agp 004 - by Bioz Stars, 2022-08
    94/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Alomone Labs hcn4 channel
    Representative confocal micrographs showing the immunolocalization of the P2X4 receptor (Apr-002, C-terminus, Alomone) and NCX1 (Anx-011, Alomone) protein in the sinoatrial node (SAN), right atria (RA) and right ventricle (RV). The SAN was identified based on its low Cx43 (green) and high <t>HCN4</t> (magenta) protein expression (left hand-side images). Images were taken from whole-mount heart preparations including the three analyzed regions, SAN, RA and RV. Dashed lines represent boundaries of the SAN. The pulmonary parenchyma was used as a structural support to facilitate immunostaining of myocardial sections and it is visible in the bottom right quadrant of each SAN image. White arrows indicate blood vessels including the SAN artery. Scale bar 30 µm. Images are representative of three different individuals.
    Hcn4 Channel, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcn4 channel/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hcn4 channel - by Bioz Stars, 2022-08
    92/100 stars
      Buy from Supplier

    95
    Alomone Labs hcn4 antibodies
    Double immunolabeling with antibodies against Hu (( a ), green signal and arrows) and <t>HCN4</t> (( b ), red signal and arrows) in the SAR. HCN4 was weakly expressed in the pacemaker cells (green, red, and white arrows) that appeared to be innervated by Hu-immunoreactive axons and nerve fibers (arrowheads). Areas of pacemaker tissue with abundant pacemaker cells are boxed in ( c ). Scale bars: 20 µm.
    Hcn4 Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcn4 antibodies/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hcn4 antibodies - by Bioz Stars, 2022-08
    95/100 stars
      Buy from Supplier

    94
    Alomone Labs guinea pig anti hcn4 antibody
    Co-labeling of Ca v 1.3 and <t>HCN4</t> in mouse SAN. ( A ) Immunolabeling of Ca v 1.3 (red) and HCN4 (green) in the isolated cranial SAN-RA preparation. ( B ) Left panels: close-up views of the area circled in ( A ); right panels: enlargement with cells expressing Ca v 1.3 or HCN4, from boxed region in left panel. ( C ) Immunolabeling of regions co-expressing Ca v 1.3 and HCN4. The left panel shows the region in accordance with the posterior nodal extension (PNL). The yellow rectangle identifies the region considered for close-up views shown in the left panels. Left panels, from top to bottom, indicate corresponding Ca v 1.3 and HCN4 immunostaining. Yellow arrows indicate SANC co-expressing Ca v 1.3 and HCN4.
    Guinea Pig Anti Hcn4 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/guinea pig anti hcn4 antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    guinea pig anti hcn4 antibody - by Bioz Stars, 2022-08
    94/100 stars
      Buy from Supplier

    93
    Alomone Labs guinea pig anti glur1 glua1 extracellular antibody
    Nogo-A regulates the synaptic insertion of calcium permeable-AMPARs. ( A , B ) Live-cell immunolabeling of surface AMPAR subunit (magenta) <t>GluA1</t> ( A ) or GluA2 ( B ) followed by immunofluorescence for presynaptic marker synapsin (Syn1/2; green) and their merged images (bottom) in primary hippocampal neurons treated for 10 min either with the control (left) or the Nogo-A function-blocking (right) antibody. For illustration, all images underwent deconvolution and were equally increased in brightness and contrast by the same absolute values for visibility. Scale bar 2 μm. ( C , D ) Normalized data for density ( C ) and fluorescence intensity ( D ) of GluA1 immuno-positive puncta in hippocampal neurons treated with either control antibody (black, n = 40) or Nogo-A function-blocking antibody (red, n = 39) for 10 min. ( E ) Normalized values for the density of GluA1 clusters colocalized with Syn 1/2 immuno-positive puncta. ( F , G ) Normalized GluA2 cluster density ( F ) and fluorescence intensity ( G ) in hippocampal neurons upon 10 min application with control antibody (black, n = 36) or Nogo-A function-blocking antibody (red, n = 35). ( H ) Normalized density of GluA2 immuno-positive puncta colocalized with Syn 1/2. Data are presented as mean ± SEM. * p
    Guinea Pig Anti Glur1 Glua1 Extracellular Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/guinea pig anti glur1 glua1 extracellular antibody/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    guinea pig anti glur1 glua1 extracellular antibody - by Bioz Stars, 2022-08
    93/100 stars
      Buy from Supplier

    Image Search Results


    Representative confocal micrographs showing the immunolocalization of the P2X4 receptor (Apr-002, C-terminus, Alomone) and NCX1 (Anx-011, Alomone) protein in the sinoatrial node (SAN), right atria (RA) and right ventricle (RV). The SAN was identified based on its low Cx43 (green) and high HCN4 (magenta) protein expression (left hand-side images). Images were taken from whole-mount heart preparations including the three analyzed regions, SAN, RA and RV. Dashed lines represent boundaries of the SAN. The pulmonary parenchyma was used as a structural support to facilitate immunostaining of myocardial sections and it is visible in the bottom right quadrant of each SAN image. White arrows indicate blood vessels including the SAN artery. Scale bar 30 µm. Images are representative of three different individuals.

    Journal: Frontiers in Pharmacology

    Article Title: The Ionotropic P2X4 Receptor has Unique Properties in the Heart by Mediating the Negative Chronotropic Effect of ATP While Increasing the Ventricular Inotropy

    doi: 10.3389/fphar.2019.01103

    Figure Lengend Snippet: Representative confocal micrographs showing the immunolocalization of the P2X4 receptor (Apr-002, C-terminus, Alomone) and NCX1 (Anx-011, Alomone) protein in the sinoatrial node (SAN), right atria (RA) and right ventricle (RV). The SAN was identified based on its low Cx43 (green) and high HCN4 (magenta) protein expression (left hand-side images). Images were taken from whole-mount heart preparations including the three analyzed regions, SAN, RA and RV. Dashed lines represent boundaries of the SAN. The pulmonary parenchyma was used as a structural support to facilitate immunostaining of myocardial sections and it is visible in the bottom right quadrant of each SAN image. White arrows indicate blood vessels including the SAN artery. Scale bar 30 µm. Images are representative of three different individuals.

    Article Snippet: Representative confocal micrographs showing the immunolocalization of the P2X4 receptor (Apr-024, extracellular loop, Alomone) and of the HCN4 channel (Agp-004, Alomone) in the rat sinoatrial node (SAN); images obtained in right atria (RA) and right ventricle (RV) are also shown for comparison.

    Techniques: Expressing, Immunostaining

    Double immunolabeling with antibodies against Hu (( a ), green signal and arrows) and HCN4 (( b ), red signal and arrows) in the SAR. HCN4 was weakly expressed in the pacemaker cells (green, red, and white arrows) that appeared to be innervated by Hu-immunoreactive axons and nerve fibers (arrowheads). Areas of pacemaker tissue with abundant pacemaker cells are boxed in ( c ). Scale bars: 20 µm.

    Journal: International Journal of Molecular Sciences

    Article Title: Structural Identification of the Pacemaker Cells and Expression of Hyperpolarization-Activated Cyclic Nucleotide-Gated (HCN) Channels in the Heart of the Wild Atlantic Cod, Gadus morhua (Linnaeus, 1758)

    doi: 10.3390/ijms22147539

    Figure Lengend Snippet: Double immunolabeling with antibodies against Hu (( a ), green signal and arrows) and HCN4 (( b ), red signal and arrows) in the SAR. HCN4 was weakly expressed in the pacemaker cells (green, red, and white arrows) that appeared to be innervated by Hu-immunoreactive axons and nerve fibers (arrowheads). Areas of pacemaker tissue with abundant pacemaker cells are boxed in ( c ). Scale bars: 20 µm.

    Article Snippet: HCN4 antibodies as reliable markers of the pacemaker cells as well as the controls of HCN4 and Islet-1 antibodies in the heart of zebrafish were reported by [ , ].

    Techniques: Immunolabeling

    Relative expression levels of hcn1 , hcn2a , hcn2b and hcn4 in sinus, atrium, ventricle and bulbus arteriosus in Atlantic cod. Transcripts were quantified by qPCR, normalized using the geometric average of ubi and eef1 expression and shown as relative values compared to hcn1 transcript levels in each sample. Data are expressed in arbitrary units (A.U.) as mean ± S.E. ( n = 5). Different superscript letters ( a, b ) indicate significant differences in transcript levels between hcn paralogues in each heart region. Differences in hcn transcript levels within each heart area were determined by one-way ANOVA with a Holm–Sidak post hoc test ( p

    Journal: International Journal of Molecular Sciences

    Article Title: Structural Identification of the Pacemaker Cells and Expression of Hyperpolarization-Activated Cyclic Nucleotide-Gated (HCN) Channels in the Heart of the Wild Atlantic Cod, Gadus morhua (Linnaeus, 1758)

    doi: 10.3390/ijms22147539

    Figure Lengend Snippet: Relative expression levels of hcn1 , hcn2a , hcn2b and hcn4 in sinus, atrium, ventricle and bulbus arteriosus in Atlantic cod. Transcripts were quantified by qPCR, normalized using the geometric average of ubi and eef1 expression and shown as relative values compared to hcn1 transcript levels in each sample. Data are expressed in arbitrary units (A.U.) as mean ± S.E. ( n = 5). Different superscript letters ( a, b ) indicate significant differences in transcript levels between hcn paralogues in each heart region. Differences in hcn transcript levels within each heart area were determined by one-way ANOVA with a Holm–Sidak post hoc test ( p

    Article Snippet: HCN4 antibodies as reliable markers of the pacemaker cells as well as the controls of HCN4 and Islet-1 antibodies in the heart of zebrafish were reported by [ , ].

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    Co-labeling of Ca v 1.3 and HCN4 in mouse SAN. ( A ) Immunolabeling of Ca v 1.3 (red) and HCN4 (green) in the isolated cranial SAN-RA preparation. ( B ) Left panels: close-up views of the area circled in ( A ); right panels: enlargement with cells expressing Ca v 1.3 or HCN4, from boxed region in left panel. ( C ) Immunolabeling of regions co-expressing Ca v 1.3 and HCN4. The left panel shows the region in accordance with the posterior nodal extension (PNL). The yellow rectangle identifies the region considered for close-up views shown in the left panels. Left panels, from top to bottom, indicate corresponding Ca v 1.3 and HCN4 immunostaining. Yellow arrows indicate SANC co-expressing Ca v 1.3 and HCN4.

    Journal: Cells

    Article Title: L-Type Cav1.3 Calcium Channels Are Required for Beta-Adrenergic Triggered Automaticity in Dormant Mouse Sinoatrial Pacemaker Cells

    doi: 10.3390/cells11071114

    Figure Lengend Snippet: Co-labeling of Ca v 1.3 and HCN4 in mouse SAN. ( A ) Immunolabeling of Ca v 1.3 (red) and HCN4 (green) in the isolated cranial SAN-RA preparation. ( B ) Left panels: close-up views of the area circled in ( A ); right panels: enlargement with cells expressing Ca v 1.3 or HCN4, from boxed region in left panel. ( C ) Immunolabeling of regions co-expressing Ca v 1.3 and HCN4. The left panel shows the region in accordance with the posterior nodal extension (PNL). The yellow rectangle identifies the region considered for close-up views shown in the left panels. Left panels, from top to bottom, indicate corresponding Ca v 1.3 and HCN4 immunostaining. Yellow arrows indicate SANC co-expressing Ca v 1.3 and HCN4.

    Article Snippet: SAN was incubated with anti-Cav1.3 (rabbit, 1:200, obtained from [ ] and anti-HCN4 (guinea pig, 1:200, Alomone labs, Cat. AGP-004) primary antibodies at 4 °C overnight.

    Techniques: Labeling, Immunolabeling, Isolation, Expressing, Immunostaining

    Expression of Ca v 1.3 and HCN4 in mouse SAN. ( A ) Immunolabeling of Ca v 1.3 (green) and HCN4 (red) in n = 64 isolated SANC with distribution of SANC based on Ca v 1.3 or HCN4 fluorescence intensity. Low fluorescence intensity is related to a lower expression of the channels while high density is related to a higher expression. ( B ) Bright-field image of a SAN-RA (N = 3) preparation used for immunolabeling of Ca v 1.3. ( C ) Immunolabeling of Ca v 1.3 (red) of the preparation in ( B ). ( D ) Immunolabeling of Ca v 1.3 in the enlarged region corresponding to the white circle in ( C ). ( E ) Close-up view of Ca v 1.3-expressing cells (red), together with nuclear labeling of DNA (blue), in the field corresponding to the central square in panel ( D ). The yellow arrow indicates Ca v 1.3 immunoreactive cells with punctate staining. Abbreviations: RA, right atrium; SVC, superior vena cava ; IVC, inferior vena cava ; IAS, inter-atrial septum; CS, coronary sinus (cut-open view); CT, crista terminalis . The white dashed line roughly indicates the CT boundary.

    Journal: Cells

    Article Title: L-Type Cav1.3 Calcium Channels Are Required for Beta-Adrenergic Triggered Automaticity in Dormant Mouse Sinoatrial Pacemaker Cells

    doi: 10.3390/cells11071114

    Figure Lengend Snippet: Expression of Ca v 1.3 and HCN4 in mouse SAN. ( A ) Immunolabeling of Ca v 1.3 (green) and HCN4 (red) in n = 64 isolated SANC with distribution of SANC based on Ca v 1.3 or HCN4 fluorescence intensity. Low fluorescence intensity is related to a lower expression of the channels while high density is related to a higher expression. ( B ) Bright-field image of a SAN-RA (N = 3) preparation used for immunolabeling of Ca v 1.3. ( C ) Immunolabeling of Ca v 1.3 (red) of the preparation in ( B ). ( D ) Immunolabeling of Ca v 1.3 in the enlarged region corresponding to the white circle in ( C ). ( E ) Close-up view of Ca v 1.3-expressing cells (red), together with nuclear labeling of DNA (blue), in the field corresponding to the central square in panel ( D ). The yellow arrow indicates Ca v 1.3 immunoreactive cells with punctate staining. Abbreviations: RA, right atrium; SVC, superior vena cava ; IVC, inferior vena cava ; IAS, inter-atrial septum; CS, coronary sinus (cut-open view); CT, crista terminalis . The white dashed line roughly indicates the CT boundary.

    Article Snippet: SAN was incubated with anti-Cav1.3 (rabbit, 1:200, obtained from [ ] and anti-HCN4 (guinea pig, 1:200, Alomone labs, Cat. AGP-004) primary antibodies at 4 °C overnight.

    Techniques: Expressing, Immunolabeling, Isolation, Fluorescence, Labeling, Staining

    Nogo-A regulates the synaptic insertion of calcium permeable-AMPARs. ( A , B ) Live-cell immunolabeling of surface AMPAR subunit (magenta) GluA1 ( A ) or GluA2 ( B ) followed by immunofluorescence for presynaptic marker synapsin (Syn1/2; green) and their merged images (bottom) in primary hippocampal neurons treated for 10 min either with the control (left) or the Nogo-A function-blocking (right) antibody. For illustration, all images underwent deconvolution and were equally increased in brightness and contrast by the same absolute values for visibility. Scale bar 2 μm. ( C , D ) Normalized data for density ( C ) and fluorescence intensity ( D ) of GluA1 immuno-positive puncta in hippocampal neurons treated with either control antibody (black, n = 40) or Nogo-A function-blocking antibody (red, n = 39) for 10 min. ( E ) Normalized values for the density of GluA1 clusters colocalized with Syn 1/2 immuno-positive puncta. ( F , G ) Normalized GluA2 cluster density ( F ) and fluorescence intensity ( G ) in hippocampal neurons upon 10 min application with control antibody (black, n = 36) or Nogo-A function-blocking antibody (red, n = 35). ( H ) Normalized density of GluA2 immuno-positive puncta colocalized with Syn 1/2. Data are presented as mean ± SEM. * p

    Journal: Cells

    Article Title: Nogo-A Modulates the Synaptic Excitation of Hippocampal Neurons in a Ca2+-Dependent Manner

    doi: 10.3390/cells10092299

    Figure Lengend Snippet: Nogo-A regulates the synaptic insertion of calcium permeable-AMPARs. ( A , B ) Live-cell immunolabeling of surface AMPAR subunit (magenta) GluA1 ( A ) or GluA2 ( B ) followed by immunofluorescence for presynaptic marker synapsin (Syn1/2; green) and their merged images (bottom) in primary hippocampal neurons treated for 10 min either with the control (left) or the Nogo-A function-blocking (right) antibody. For illustration, all images underwent deconvolution and were equally increased in brightness and contrast by the same absolute values for visibility. Scale bar 2 μm. ( C , D ) Normalized data for density ( C ) and fluorescence intensity ( D ) of GluA1 immuno-positive puncta in hippocampal neurons treated with either control antibody (black, n = 40) or Nogo-A function-blocking antibody (red, n = 39) for 10 min. ( E ) Normalized values for the density of GluA1 clusters colocalized with Syn 1/2 immuno-positive puncta. ( F , G ) Normalized GluA2 cluster density ( F ) and fluorescence intensity ( G ) in hippocampal neurons upon 10 min application with control antibody (black, n = 36) or Nogo-A function-blocking antibody (red, n = 35). ( H ) Normalized density of GluA2 immuno-positive puncta colocalized with Syn 1/2. Data are presented as mean ± SEM. * p

    Article Snippet: In the case of the AMPA receptors, the anti-AMPAR 1 GluA1 (Alomone Labs, Jerusalem, Israel, Cat# AGP-009, 1:50) and anti-AMPAR 2 GluA2 (Alomone Labs, Cat# AGC-005, 1:50) were co-applied with the Nogo-A or control antibodies for 10 min. After completion of the treatment, the coverslips were rinsed with pre-warmed NB- medium and fixed with 4% paraformaldehyde (PFA) in phosphate buffer (PB containing in mM 50 NaH2 PO4 *2H2 O, 85 Na2 HPO4 *2H2 O) for 10 min at room temperature (RT).

    Techniques: Immunolabeling, Immunofluorescence, Marker, Blocking Assay, Fluorescence