p2x4 antibody  (Alomone Labs)


Bioz Verified Symbol Alomone Labs is a verified supplier
Bioz Manufacturer Symbol Alomone Labs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Name:
    Anti P2X4 Receptor Antibody
    Description:
    Anti P2X4 Receptor Antibody APR 002 is a highly specific antibody directed against an epitope of the rat protein The antibody can be used in western blot immunocytochemistry immunohistochemistry immunoprecipitation and indirect flow cytometry applications It has been designed to recognize P2X4 purinergic receptor from rat mouse and human samples
    Catalog Number:
    APR-002
    Price:
    397.0
    Category:
    Primary Antibody
    Applications:
    Immunocytochemistry, Immunofluorescence, Indirect Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    25 mcl
    Antibody Type:
    Polyclonal Primary Antibodies
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
    Buy from Supplier


    Structured Review

    Alomone Labs p2x4 antibody
    Anti P2X4 Receptor Antibody
    Anti P2X4 Receptor Antibody APR 002 is a highly specific antibody directed against an epitope of the rat protein The antibody can be used in western blot immunocytochemistry immunohistochemistry immunoprecipitation and indirect flow cytometry applications It has been designed to recognize P2X4 purinergic receptor from rat mouse and human samples
    https://www.bioz.com/result/p2x4 antibody/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p2x4 antibody - by Bioz Stars, 2021-09
    95/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Cystitis-Related Bladder Pain Involves ATP-Dependent HMGB1 Release from Macrophages and Its Downstream H2S/Cav3.2 Signaling in Mice
    Article Snippet: .. After incubation for 48 h, protein levels of P2X4 or P2X7 were determined by Western blotting, using an anti-P2X4 rabbit polyclonal antibody (Alomone Labs) (1:1000 dilution) and an anti-P2X7 rabbit polyclonal antibody (alomone labs) (1:3000 dilution) as the primary antibodies, and an HRP-conjugated anti-rabbit IgG antibody (Chemicon International) (1:5000 dilution) as the secondary antibody. ..

    Article Title: Inhibition of P2X4R attenuates white matter injury in mice after intracerebral hemorrhage by regulating microglial phenotypes
    Article Snippet: .. The membranes were blocked with 5% milk at room temperature for 1 h and incubated overnight with the following primary antibodies: P2X4R (1:1000; APR-002; Alomone), MBP (1:1000; CST#83683S; Cell Signaling Technology), amyloid precursor protein (APP) (1:1000; 32136; Abcam), BDNF (1:1000; 28205-1-AP; Proteintech), p-TrkB (1:1000; ABN1381; Millipore), TrkB (1:100; sc-7268; Santa Cruz), P2X7R (1:1000; APR-004; Alomone), and β-actin (1:5000, 8226; Abcam). ..

    Article Title: Astragalin Alleviates Neuropathic Pain by Suppressing P2X4-Mediated Signaling in the Dorsal Root Ganglia of Rats
    Article Snippet: .. Sections were incubated overnight at 4°C with mouse anti-GFAP (1:200 dilution; 38014, Signalway Antibody, Nanjing, China) and rabbit anti-P2X4 (1:200; APR-002, Alomone Labs, Jerusalem, Israel) primary antibodies and washed three times for 10 min each in PBS ( ). ..

    Article Title: P2X4 Purinergic Receptors as a Therapeutic Target in Aggressive Prostate Cancer
    Article Snippet: .. Slides were steamed for 25 minutes in antigen retrieval solution (Cat. No., H-3300, Vector Laboratories, INC. Burlingame, CA) and incubated with rabbit polyclonal anti-human P2X4 (Cat. No. HPA039494, Sigma-Aldrich, St. Louis, MO), rabbit polyclonal anti-mouse P2X4 (Cat. No. APR-002, Alomone Labs, Jerusalem, Israel), rabbit monoclonal anti-androgen receptor antibody (Cat. No. 5153, Cell Signaling, Danvers, MA), rabbit monoclonal anti-CD11b (Cat. No. 6410, Bio SB, Santa Barbara, CA.), or rabbit monoclonal anti-CD3 (Cat. No. RM-9107-S0, ThermoFisher Scientific, Waltham, MA) for 45 minutes at RT. ..

    Article Title: Inhibition of P2X4R attenuates white matter injury in mice after intracerebral hemorrhage by regulating microglial phenotypes
    Article Snippet: .. After washing with PBS and incubation with 10% donkey serum containing 0.3% Triton X-100 for 1 h at room temperature, the sections were incubated with primary antibodies overnight at 4 °C, including P2X4R (1:200, Alomone, APR-002), Iba-1 (1:500, Abcam, 5076), NeuN (1:1000, Abcam, 177487), GFAP (1:500, Millipore, MAB360), MBP (1:500, Cell Signaling Technology, CST#83683S), Neurofilament-200 (NF-200) (1:1000, Sigma, N4142), CD16/32 (1:100, Abcam, 24235), and Arg-1(1:500, Proteintech, 16001-1-AP). ..

    Article Title: Astragalin Alleviates Neuropathic Pain by Suppressing P2X4-Mediated Signaling in the Dorsal Root Ganglia of Rats
    Article Snippet: .. Non-specific antigens were blocked by incubating the membrane for 2 h at room temperature in 5% skim milk; this was followed by overnight incubation at 4°C with the following primary antibodies: rabbit anti-P2X4 (1:500; Alomone Labs), rabbit anti-ERK1/2 (1:1,000), and anti-phosphorylated (p)ERK1/2 (1:2,000) (both from Cell Signaling Technology, Danvers, MA, United States); and rabbit anti-tumor necrosis factor receptor (TNF-R)1 (1:800) and mouse anti-GFAP (1:800) (both from Abcam, Cambridge, MA, United States). ..

    Western Blot:

    Article Title: Cystitis-Related Bladder Pain Involves ATP-Dependent HMGB1 Release from Macrophages and Its Downstream H2S/Cav3.2 Signaling in Mice
    Article Snippet: .. After incubation for 48 h, protein levels of P2X4 or P2X7 were determined by Western blotting, using an anti-P2X4 rabbit polyclonal antibody (Alomone Labs) (1:1000 dilution) and an anti-P2X7 rabbit polyclonal antibody (alomone labs) (1:3000 dilution) as the primary antibodies, and an HRP-conjugated anti-rabbit IgG antibody (Chemicon International) (1:5000 dilution) as the secondary antibody. ..

    Binding Assay:

    Article Title: P2X4 Purinergic Receptors as a Therapeutic Target in Aggressive Prostate Cancer
    Article Snippet: .. Blots were probed with antibody specific for human P2X4 (Cat. No. HPA039494, Sigma-Aldrich, St. Louis, MO), mouse P2X4 (Cat. No. APR-002, Alomone Labs, Jerusalem, Israel), E-cadherin (Cat. No. 3195, Cell Signaling, Danvers, MA.), Vimentin (Cat. No. 5741, Cell Signaling, Danvers, MA), and cleaved caspase-3 (Cat. No. 9664, Cell Signaling, Danvers, MA). β-Actin (Cat. No. A5441, Sigma-Aldrich, St. Louis, MO) or TATA binding protein (Cat. No. 51841, Abcam, Cambridge, MA) were used to ensure equal loading of proteins in each lane. ..

    other:

    Article Title: Biophysical basis for Kv1.3 regulation of membrane potential changes induced by P2X4‐mediated calcium entry in microglia, et al. Biophysical basis for Kv1.3 regulation of membrane potential changes induced by P2X4‐mediated calcium entry in microglia
    Article Snippet: Validation of polyclonal rabbit anti‐P2X4 antibody.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Alomone Labs p2x4 antibody
    P2x4 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p2x4 antibody/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p2x4 antibody - by Bioz Stars, 2021-09
    95/100 stars
      Buy from Supplier

    Image Search Results