fgf21 antibody  (Boster Bio)


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    Boster Bio fgf21 antibody
    Effects of IL-1β on β-Klotho and <t>FGF21</t> in Huh-7 cells. Huh-7 cells were incubated with IL-1β for 6 hours. β-Klotho expression levels (A and G), protein kinase B (B), extracelluar signal-regulated kinases (C), JNK (D), IκBα (E), and FGF21 (F and G) were determined by immunoblotting. IL, interleukin; FGF, fibroblast growth factor; JNK, c-Jun N-terminal kinase.
    Fgf21 Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fgf21 antibody/product/Boster Bio
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    fgf21 antibody - by Bioz Stars, 2022-10
    90/100 stars

    Images

    1) Product Images from "Expression of Fibroblast Growth Factor 21 and β-Klotho Regulates Hepatic Fibrosis through the Nuclear Factor-κB and c-Jun N-Terminal Kinase Pathways"

    Article Title: Expression of Fibroblast Growth Factor 21 and β-Klotho Regulates Hepatic Fibrosis through the Nuclear Factor-κB and c-Jun N-Terminal Kinase Pathways

    Journal: Gut and Liver

    doi: 10.5009/gnl17443

    Effects of IL-1β on β-Klotho and FGF21 in Huh-7 cells. Huh-7 cells were incubated with IL-1β for 6 hours. β-Klotho expression levels (A and G), protein kinase B (B), extracelluar signal-regulated kinases (C), JNK (D), IκBα (E), and FGF21 (F and G) were determined by immunoblotting. IL, interleukin; FGF, fibroblast growth factor; JNK, c-Jun N-terminal kinase.
    Figure Legend Snippet: Effects of IL-1β on β-Klotho and FGF21 in Huh-7 cells. Huh-7 cells were incubated with IL-1β for 6 hours. β-Klotho expression levels (A and G), protein kinase B (B), extracelluar signal-regulated kinases (C), JNK (D), IκBα (E), and FGF21 (F and G) were determined by immunoblotting. IL, interleukin; FGF, fibroblast growth factor; JNK, c-Jun N-terminal kinase.

    Techniques Used: Incubation, Expressing

    FGF21 inhibits IL-1β-induced growth retardation of hepatocytes. Huh-7 cells were incubated with 10 ng/mL of IL-1β (A), 500 ng/mL of FGF21 (B), or IL-1β+FGF21 (C), after which the expression of β-Klotho and PCNA was determined. (D) IL-1β-treated Huh-7 cells were incubated with or without FGF21 in a dose-dependent manner. Cell viability was measured by an methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay. IL, interleukin; FGF, fibroblast growth factor; PCNA, proliferating cell nuclear antigen; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
    Figure Legend Snippet: FGF21 inhibits IL-1β-induced growth retardation of hepatocytes. Huh-7 cells were incubated with 10 ng/mL of IL-1β (A), 500 ng/mL of FGF21 (B), or IL-1β+FGF21 (C), after which the expression of β-Klotho and PCNA was determined. (D) IL-1β-treated Huh-7 cells were incubated with or without FGF21 in a dose-dependent manner. Cell viability was measured by an methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay. IL, interleukin; FGF, fibroblast growth factor; PCNA, proliferating cell nuclear antigen; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

    Techniques Used: Incubation, Expressing, MTT Assay

    Analysis of serum FGF19 and FGF21 levels in patients with viral or alcoholic hepatitis. Serum levels of FGF19 (A) and FGF21 (B) were determined by enzyme-linked immunosorbent assay. G1 (F0–F1, n=10), G2 (F2–F3, n=10), G3 (F4A–F4C, n=15). FGF, fibroblast growth factor. *p
    Figure Legend Snippet: Analysis of serum FGF19 and FGF21 levels in patients with viral or alcoholic hepatitis. Serum levels of FGF19 (A) and FGF21 (B) were determined by enzyme-linked immunosorbent assay. G1 (F0–F1, n=10), G2 (F2–F3, n=10), G3 (F4A–F4C, n=15). FGF, fibroblast growth factor. *p

    Techniques Used: Enzyme-linked Immunosorbent Assay

    Analysis of FGF19, FGF21, and β-Klotho expression and inflammatory markers in liver tissue. Expression levels of mRNA for FGF19 (A), FGF21 (B), β-Klotho (C), IL-1β (D), IL-6 (E), and TNF-α (F) were determined by real-time polymerase chain reaction. G1 (F0–F1, n=10), G2 (F2–F3, n=10), G3 (F4A–F4C, n=15). FGF, fibroblast growth factor; IL, interleukin; TNF, tumor necrosis factor. *p
    Figure Legend Snippet: Analysis of FGF19, FGF21, and β-Klotho expression and inflammatory markers in liver tissue. Expression levels of mRNA for FGF19 (A), FGF21 (B), β-Klotho (C), IL-1β (D), IL-6 (E), and TNF-α (F) were determined by real-time polymerase chain reaction. G1 (F0–F1, n=10), G2 (F2–F3, n=10), G3 (F4A–F4C, n=15). FGF, fibroblast growth factor; IL, interleukin; TNF, tumor necrosis factor. *p

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction

    Signaling pathways that inhibit β-Klotho and induce FGF21 by IL-1β in Huh-7 cells. Huh-7 cells were pretreated with an NF-κB inhibitor (Bay11-7082, A and E), JNK inhibitor (SP600125, B and F), protein kinase B inhibitor (LY294002, C and G), or extracelluar signal-regulated kinases inhibitor (PD98059, D and H) for 20 minutes and then treated with 10 ng/mL of IL-1β for 6 hours to detect β-Klotho and FGF21. (I) Huh-7 cells were pretreated with 1 μM NF-κB inhibitor Bay11-7082, after which β-Klotho and FGF21 levels were determined by immunoblotting. (J) Huh-7 cells were pretreated with 0.5 μM JNK inhibitor SP600125, after which β-Klotho and FGF21 levels were determined by immunoblotting. (K) β-Klotho and FGF21 levels were assessed 6 hours after pretreatment with 1 μM NF-κB inhibitor Bay11-7082 in Huh-7 cells. (L) β-Klotho and FGF21 levels were assessed 6 hours after pretreatment with 0.5 μM JNK inhibitor SP600125 in Huh-7 cells. FGF, fibroblast growth factor; IL, interleukin; NF-κB, nuclear factor-κB; JNK, c-Jun N-terminal kinase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
    Figure Legend Snippet: Signaling pathways that inhibit β-Klotho and induce FGF21 by IL-1β in Huh-7 cells. Huh-7 cells were pretreated with an NF-κB inhibitor (Bay11-7082, A and E), JNK inhibitor (SP600125, B and F), protein kinase B inhibitor (LY294002, C and G), or extracelluar signal-regulated kinases inhibitor (PD98059, D and H) for 20 minutes and then treated with 10 ng/mL of IL-1β for 6 hours to detect β-Klotho and FGF21. (I) Huh-7 cells were pretreated with 1 μM NF-κB inhibitor Bay11-7082, after which β-Klotho and FGF21 levels were determined by immunoblotting. (J) Huh-7 cells were pretreated with 0.5 μM JNK inhibitor SP600125, after which β-Klotho and FGF21 levels were determined by immunoblotting. (K) β-Klotho and FGF21 levels were assessed 6 hours after pretreatment with 1 μM NF-κB inhibitor Bay11-7082 in Huh-7 cells. (L) β-Klotho and FGF21 levels were assessed 6 hours after pretreatment with 0.5 μM JNK inhibitor SP600125 in Huh-7 cells. FGF, fibroblast growth factor; IL, interleukin; NF-κB, nuclear factor-κB; JNK, c-Jun N-terminal kinase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

    Techniques Used:

    2) Product Images from "Expression of Fibroblast Growth Factor 21 and β-Klotho Regulates Hepatic Fibrosis through the Nuclear Factor-κB and c-Jun N-Terminal Kinase Pathways"

    Article Title: Expression of Fibroblast Growth Factor 21 and β-Klotho Regulates Hepatic Fibrosis through the Nuclear Factor-κB and c-Jun N-Terminal Kinase Pathways

    Journal: Gut and Liver

    doi: 10.5009/gnl17443

    Effects of IL-1β on β-Klotho and FGF21 in Huh-7 cells. Huh-7 cells were incubated with IL-1β for 6 hours. β-Klotho expression levels (A and G), protein kinase B (B), extracelluar signal-regulated kinases (C), JNK (D), IκBα (E), and FGF21 (F and G) were determined by immunoblotting. IL, interleukin; FGF, fibroblast growth factor; JNK, c-Jun N-terminal kinase.
    Figure Legend Snippet: Effects of IL-1β on β-Klotho and FGF21 in Huh-7 cells. Huh-7 cells were incubated with IL-1β for 6 hours. β-Klotho expression levels (A and G), protein kinase B (B), extracelluar signal-regulated kinases (C), JNK (D), IκBα (E), and FGF21 (F and G) were determined by immunoblotting. IL, interleukin; FGF, fibroblast growth factor; JNK, c-Jun N-terminal kinase.

    Techniques Used: Incubation, Expressing

    FGF21 inhibits IL-1β-induced growth retardation of hepatocytes. Huh-7 cells were incubated with 10 ng/mL of IL-1β (A), 500 ng/mL of FGF21 (B), or IL-1β+FGF21 (C), after which the expression of β-Klotho and PCNA was determined. (D) IL-1β-treated Huh-7 cells were incubated with or without FGF21 in a dose-dependent manner. Cell viability was measured by an methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay. IL, interleukin; FGF, fibroblast growth factor; PCNA, proliferating cell nuclear antigen; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
    Figure Legend Snippet: FGF21 inhibits IL-1β-induced growth retardation of hepatocytes. Huh-7 cells were incubated with 10 ng/mL of IL-1β (A), 500 ng/mL of FGF21 (B), or IL-1β+FGF21 (C), after which the expression of β-Klotho and PCNA was determined. (D) IL-1β-treated Huh-7 cells were incubated with or without FGF21 in a dose-dependent manner. Cell viability was measured by an methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay. IL, interleukin; FGF, fibroblast growth factor; PCNA, proliferating cell nuclear antigen; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

    Techniques Used: Incubation, Expressing, MTT Assay

    Analysis of serum FGF19 and FGF21 levels in patients with viral or alcoholic hepatitis. Serum levels of FGF19 (A) and FGF21 (B) were determined by enzyme-linked immunosorbent assay. G1 (F0–F1, n=10), G2 (F2–F3, n=10), G3 (F4A–F4C, n=15). FGF, fibroblast growth factor. *p
    Figure Legend Snippet: Analysis of serum FGF19 and FGF21 levels in patients with viral or alcoholic hepatitis. Serum levels of FGF19 (A) and FGF21 (B) were determined by enzyme-linked immunosorbent assay. G1 (F0–F1, n=10), G2 (F2–F3, n=10), G3 (F4A–F4C, n=15). FGF, fibroblast growth factor. *p

    Techniques Used: Enzyme-linked Immunosorbent Assay

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    Boster Bio fgf21 antibody
    Effects of IL-1β on β-Klotho and <t>FGF21</t> in Huh-7 cells. Huh-7 cells were incubated with IL-1β for 6 hours. β-Klotho expression levels (A and G), protein kinase B (B), extracelluar signal-regulated kinases (C), JNK (D), IκBα (E), and FGF21 (F and G) were determined by immunoblotting. IL, interleukin; FGF, fibroblast growth factor; JNK, c-Jun N-terminal kinase.
    Fgf21 Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fgf21 antibody/product/Boster Bio
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fgf21 antibody - by Bioz Stars, 2022-10
    90/100 stars
      Buy from Supplier

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    Effects of IL-1β on β-Klotho and FGF21 in Huh-7 cells. Huh-7 cells were incubated with IL-1β for 6 hours. β-Klotho expression levels (A and G), protein kinase B (B), extracelluar signal-regulated kinases (C), JNK (D), IκBα (E), and FGF21 (F and G) were determined by immunoblotting. IL, interleukin; FGF, fibroblast growth factor; JNK, c-Jun N-terminal kinase.

    Journal: Gut and Liver

    Article Title: Expression of Fibroblast Growth Factor 21 and β-Klotho Regulates Hepatic Fibrosis through the Nuclear Factor-κB and c-Jun N-Terminal Kinase Pathways

    doi: 10.5009/gnl17443

    Figure Lengend Snippet: Effects of IL-1β on β-Klotho and FGF21 in Huh-7 cells. Huh-7 cells were incubated with IL-1β for 6 hours. β-Klotho expression levels (A and G), protein kinase B (B), extracelluar signal-regulated kinases (C), JNK (D), IκBα (E), and FGF21 (F and G) were determined by immunoblotting. IL, interleukin; FGF, fibroblast growth factor; JNK, c-Jun N-terminal kinase.

    Article Snippet: The lysates were subjected to Western blotting analysis under reducing conditions using previously validated human β-Klotho antibodies (R & D Systems), anti-phospho-Erk1/2, total Erk1/2, phospho-AKT, total AKT, phospho-JNK, and total JNK antibodies (Cell Signaling Technology, Danvers, MA, USA), phospho-IκBα, total IκBα, proliferating cell nuclear antigen (PCNA), and GAPDH antibodies (Santa Cruz Biotechnology, Santa Cruz, CA, USA), and FGF21 antibody (Boster Biological Technology, Pleasanton, CA, USA).

    Techniques: Incubation, Expressing

    FGF21 inhibits IL-1β-induced growth retardation of hepatocytes. Huh-7 cells were incubated with 10 ng/mL of IL-1β (A), 500 ng/mL of FGF21 (B), or IL-1β+FGF21 (C), after which the expression of β-Klotho and PCNA was determined. (D) IL-1β-treated Huh-7 cells were incubated with or without FGF21 in a dose-dependent manner. Cell viability was measured by an methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay. IL, interleukin; FGF, fibroblast growth factor; PCNA, proliferating cell nuclear antigen; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

    Journal: Gut and Liver

    Article Title: Expression of Fibroblast Growth Factor 21 and β-Klotho Regulates Hepatic Fibrosis through the Nuclear Factor-κB and c-Jun N-Terminal Kinase Pathways

    doi: 10.5009/gnl17443

    Figure Lengend Snippet: FGF21 inhibits IL-1β-induced growth retardation of hepatocytes. Huh-7 cells were incubated with 10 ng/mL of IL-1β (A), 500 ng/mL of FGF21 (B), or IL-1β+FGF21 (C), after which the expression of β-Klotho and PCNA was determined. (D) IL-1β-treated Huh-7 cells were incubated with or without FGF21 in a dose-dependent manner. Cell viability was measured by an methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay. IL, interleukin; FGF, fibroblast growth factor; PCNA, proliferating cell nuclear antigen; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

    Article Snippet: The lysates were subjected to Western blotting analysis under reducing conditions using previously validated human β-Klotho antibodies (R & D Systems), anti-phospho-Erk1/2, total Erk1/2, phospho-AKT, total AKT, phospho-JNK, and total JNK antibodies (Cell Signaling Technology, Danvers, MA, USA), phospho-IκBα, total IκBα, proliferating cell nuclear antigen (PCNA), and GAPDH antibodies (Santa Cruz Biotechnology, Santa Cruz, CA, USA), and FGF21 antibody (Boster Biological Technology, Pleasanton, CA, USA).

    Techniques: Incubation, Expressing, MTT Assay

    Analysis of serum FGF19 and FGF21 levels in patients with viral or alcoholic hepatitis. Serum levels of FGF19 (A) and FGF21 (B) were determined by enzyme-linked immunosorbent assay. G1 (F0–F1, n=10), G2 (F2–F3, n=10), G3 (F4A–F4C, n=15). FGF, fibroblast growth factor. *p

    Journal: Gut and Liver

    Article Title: Expression of Fibroblast Growth Factor 21 and β-Klotho Regulates Hepatic Fibrosis through the Nuclear Factor-κB and c-Jun N-Terminal Kinase Pathways

    doi: 10.5009/gnl17443

    Figure Lengend Snippet: Analysis of serum FGF19 and FGF21 levels in patients with viral or alcoholic hepatitis. Serum levels of FGF19 (A) and FGF21 (B) were determined by enzyme-linked immunosorbent assay. G1 (F0–F1, n=10), G2 (F2–F3, n=10), G3 (F4A–F4C, n=15). FGF, fibroblast growth factor. *p

    Article Snippet: The lysates were subjected to Western blotting analysis under reducing conditions using previously validated human β-Klotho antibodies (R & D Systems), anti-phospho-Erk1/2, total Erk1/2, phospho-AKT, total AKT, phospho-JNK, and total JNK antibodies (Cell Signaling Technology, Danvers, MA, USA), phospho-IκBα, total IκBα, proliferating cell nuclear antigen (PCNA), and GAPDH antibodies (Santa Cruz Biotechnology, Santa Cruz, CA, USA), and FGF21 antibody (Boster Biological Technology, Pleasanton, CA, USA).

    Techniques: Enzyme-linked Immunosorbent Assay

    Analysis of FGF19, FGF21, and β-Klotho expression and inflammatory markers in liver tissue. Expression levels of mRNA for FGF19 (A), FGF21 (B), β-Klotho (C), IL-1β (D), IL-6 (E), and TNF-α (F) were determined by real-time polymerase chain reaction. G1 (F0–F1, n=10), G2 (F2–F3, n=10), G3 (F4A–F4C, n=15). FGF, fibroblast growth factor; IL, interleukin; TNF, tumor necrosis factor. *p

    Journal: Gut and Liver

    Article Title: Expression of Fibroblast Growth Factor 21 and β-Klotho Regulates Hepatic Fibrosis through the Nuclear Factor-κB and c-Jun N-Terminal Kinase Pathways

    doi: 10.5009/gnl17443

    Figure Lengend Snippet: Analysis of FGF19, FGF21, and β-Klotho expression and inflammatory markers in liver tissue. Expression levels of mRNA for FGF19 (A), FGF21 (B), β-Klotho (C), IL-1β (D), IL-6 (E), and TNF-α (F) were determined by real-time polymerase chain reaction. G1 (F0–F1, n=10), G2 (F2–F3, n=10), G3 (F4A–F4C, n=15). FGF, fibroblast growth factor; IL, interleukin; TNF, tumor necrosis factor. *p

    Article Snippet: The lysates were subjected to Western blotting analysis under reducing conditions using previously validated human β-Klotho antibodies (R & D Systems), anti-phospho-Erk1/2, total Erk1/2, phospho-AKT, total AKT, phospho-JNK, and total JNK antibodies (Cell Signaling Technology, Danvers, MA, USA), phospho-IκBα, total IκBα, proliferating cell nuclear antigen (PCNA), and GAPDH antibodies (Santa Cruz Biotechnology, Santa Cruz, CA, USA), and FGF21 antibody (Boster Biological Technology, Pleasanton, CA, USA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    Signaling pathways that inhibit β-Klotho and induce FGF21 by IL-1β in Huh-7 cells. Huh-7 cells were pretreated with an NF-κB inhibitor (Bay11-7082, A and E), JNK inhibitor (SP600125, B and F), protein kinase B inhibitor (LY294002, C and G), or extracelluar signal-regulated kinases inhibitor (PD98059, D and H) for 20 minutes and then treated with 10 ng/mL of IL-1β for 6 hours to detect β-Klotho and FGF21. (I) Huh-7 cells were pretreated with 1 μM NF-κB inhibitor Bay11-7082, after which β-Klotho and FGF21 levels were determined by immunoblotting. (J) Huh-7 cells were pretreated with 0.5 μM JNK inhibitor SP600125, after which β-Klotho and FGF21 levels were determined by immunoblotting. (K) β-Klotho and FGF21 levels were assessed 6 hours after pretreatment with 1 μM NF-κB inhibitor Bay11-7082 in Huh-7 cells. (L) β-Klotho and FGF21 levels were assessed 6 hours after pretreatment with 0.5 μM JNK inhibitor SP600125 in Huh-7 cells. FGF, fibroblast growth factor; IL, interleukin; NF-κB, nuclear factor-κB; JNK, c-Jun N-terminal kinase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

    Journal: Gut and Liver

    Article Title: Expression of Fibroblast Growth Factor 21 and β-Klotho Regulates Hepatic Fibrosis through the Nuclear Factor-κB and c-Jun N-Terminal Kinase Pathways

    doi: 10.5009/gnl17443

    Figure Lengend Snippet: Signaling pathways that inhibit β-Klotho and induce FGF21 by IL-1β in Huh-7 cells. Huh-7 cells were pretreated with an NF-κB inhibitor (Bay11-7082, A and E), JNK inhibitor (SP600125, B and F), protein kinase B inhibitor (LY294002, C and G), or extracelluar signal-regulated kinases inhibitor (PD98059, D and H) for 20 minutes and then treated with 10 ng/mL of IL-1β for 6 hours to detect β-Klotho and FGF21. (I) Huh-7 cells were pretreated with 1 μM NF-κB inhibitor Bay11-7082, after which β-Klotho and FGF21 levels were determined by immunoblotting. (J) Huh-7 cells were pretreated with 0.5 μM JNK inhibitor SP600125, after which β-Klotho and FGF21 levels were determined by immunoblotting. (K) β-Klotho and FGF21 levels were assessed 6 hours after pretreatment with 1 μM NF-κB inhibitor Bay11-7082 in Huh-7 cells. (L) β-Klotho and FGF21 levels were assessed 6 hours after pretreatment with 0.5 μM JNK inhibitor SP600125 in Huh-7 cells. FGF, fibroblast growth factor; IL, interleukin; NF-κB, nuclear factor-κB; JNK, c-Jun N-terminal kinase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

    Article Snippet: The lysates were subjected to Western blotting analysis under reducing conditions using previously validated human β-Klotho antibodies (R & D Systems), anti-phospho-Erk1/2, total Erk1/2, phospho-AKT, total AKT, phospho-JNK, and total JNK antibodies (Cell Signaling Technology, Danvers, MA, USA), phospho-IκBα, total IκBα, proliferating cell nuclear antigen (PCNA), and GAPDH antibodies (Santa Cruz Biotechnology, Santa Cruz, CA, USA), and FGF21 antibody (Boster Biological Technology, Pleasanton, CA, USA).

    Techniques:

    Effects of IL-1β on β-Klotho and FGF21 in Huh-7 cells. Huh-7 cells were incubated with IL-1β for 6 hours. β-Klotho expression levels (A and G), protein kinase B (B), extracelluar signal-regulated kinases (C), JNK (D), IκBα (E), and FGF21 (F and G) were determined by immunoblotting. IL, interleukin; FGF, fibroblast growth factor; JNK, c-Jun N-terminal kinase.

    Journal: Gut and Liver

    Article Title: Expression of Fibroblast Growth Factor 21 and β-Klotho Regulates Hepatic Fibrosis through the Nuclear Factor-κB and c-Jun N-Terminal Kinase Pathways

    doi: 10.5009/gnl17443

    Figure Lengend Snippet: Effects of IL-1β on β-Klotho and FGF21 in Huh-7 cells. Huh-7 cells were incubated with IL-1β for 6 hours. β-Klotho expression levels (A and G), protein kinase B (B), extracelluar signal-regulated kinases (C), JNK (D), IκBα (E), and FGF21 (F and G) were determined by immunoblotting. IL, interleukin; FGF, fibroblast growth factor; JNK, c-Jun N-terminal kinase.

    Article Snippet: The lysates were subjected to Western blotting analysis under reducing conditions using previously validated human β-Klotho antibodies (R & D Systems), anti-phospho-Erk1/2, total Erk1/2, phospho-AKT, total AKT, phospho-JNK, and total JNK antibodies (Cell Signaling Technology, Danvers, MA, USA), phospho-IκBα, total IκBα, proliferating cell nuclear antigen (PCNA), and GAPDH antibodies (Santa Cruz Biotechnology, Santa Cruz, CA, USA), and FGF21 antibody (Boster Biological Technology, Pleasanton, CA, USA).

    Techniques: Incubation, Expressing

    FGF21 inhibits IL-1β-induced growth retardation of hepatocytes. Huh-7 cells were incubated with 10 ng/mL of IL-1β (A), 500 ng/mL of FGF21 (B), or IL-1β+FGF21 (C), after which the expression of β-Klotho and PCNA was determined. (D) IL-1β-treated Huh-7 cells were incubated with or without FGF21 in a dose-dependent manner. Cell viability was measured by an methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay. IL, interleukin; FGF, fibroblast growth factor; PCNA, proliferating cell nuclear antigen; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

    Journal: Gut and Liver

    Article Title: Expression of Fibroblast Growth Factor 21 and β-Klotho Regulates Hepatic Fibrosis through the Nuclear Factor-κB and c-Jun N-Terminal Kinase Pathways

    doi: 10.5009/gnl17443

    Figure Lengend Snippet: FGF21 inhibits IL-1β-induced growth retardation of hepatocytes. Huh-7 cells were incubated with 10 ng/mL of IL-1β (A), 500 ng/mL of FGF21 (B), or IL-1β+FGF21 (C), after which the expression of β-Klotho and PCNA was determined. (D) IL-1β-treated Huh-7 cells were incubated with or without FGF21 in a dose-dependent manner. Cell viability was measured by an methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay. IL, interleukin; FGF, fibroblast growth factor; PCNA, proliferating cell nuclear antigen; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

    Article Snippet: The lysates were subjected to Western blotting analysis under reducing conditions using previously validated human β-Klotho antibodies (R & D Systems), anti-phospho-Erk1/2, total Erk1/2, phospho-AKT, total AKT, phospho-JNK, and total JNK antibodies (Cell Signaling Technology, Danvers, MA, USA), phospho-IκBα, total IκBα, proliferating cell nuclear antigen (PCNA), and GAPDH antibodies (Santa Cruz Biotechnology, Santa Cruz, CA, USA), and FGF21 antibody (Boster Biological Technology, Pleasanton, CA, USA).

    Techniques: Incubation, Expressing, MTT Assay

    Analysis of serum FGF19 and FGF21 levels in patients with viral or alcoholic hepatitis. Serum levels of FGF19 (A) and FGF21 (B) were determined by enzyme-linked immunosorbent assay. G1 (F0–F1, n=10), G2 (F2–F3, n=10), G3 (F4A–F4C, n=15). FGF, fibroblast growth factor. *p

    Journal: Gut and Liver

    Article Title: Expression of Fibroblast Growth Factor 21 and β-Klotho Regulates Hepatic Fibrosis through the Nuclear Factor-κB and c-Jun N-Terminal Kinase Pathways

    doi: 10.5009/gnl17443

    Figure Lengend Snippet: Analysis of serum FGF19 and FGF21 levels in patients with viral or alcoholic hepatitis. Serum levels of FGF19 (A) and FGF21 (B) were determined by enzyme-linked immunosorbent assay. G1 (F0–F1, n=10), G2 (F2–F3, n=10), G3 (F4A–F4C, n=15). FGF, fibroblast growth factor. *p

    Article Snippet: The lysates were subjected to Western blotting analysis under reducing conditions using previously validated human β-Klotho antibodies (R & D Systems), anti-phospho-Erk1/2, total Erk1/2, phospho-AKT, total AKT, phospho-JNK, and total JNK antibodies (Cell Signaling Technology, Danvers, MA, USA), phospho-IκBα, total IκBα, proliferating cell nuclear antigen (PCNA), and GAPDH antibodies (Santa Cruz Biotechnology, Santa Cruz, CA, USA), and FGF21 antibody (Boster Biological Technology, Pleasanton, CA, USA).

    Techniques: Enzyme-linked Immunosorbent Assay