96 well microdialysis plate  (Thermo Fisher)


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    Name:
    Pierce 96 well Microdialysis Plate
    Description:
    Thermo Scientific Pierce 96 well 10K MWCO Microdialysis Plates are automation compatible microplate dialysis devices for rapidly processing 1 to 96 samples with volumes 10 to 100 µL This kit comes with 12 cartridges of eight microdialysis devices in a 96 deep well plate Features of Microdialysis Plates • High yield greater than 90 protein recovery after dialysis • Rapid dialysis completed in 2 to 4 hours • Easy to use sample loading and retrieval can be done with a standard pipette • Flexible detachable 8 device strips scalable from 1 to 96 samples • Automation compatible plate format conforms to the SBS Microplate Standard Each sample processed with the Pierce 96 well Microdialysis Dialysis Plate is dialyzed inside a single removable dialysis device created by two regenerated cellulose membranes separated by 2mm The short diffusion distance and large surface area facilitates rapid dialysis with the removal of salts and small molecules often completed in 2 to 4 hours Sample recovery is performed using standard laboratory micropipettes Individual microdialysis devices can also be used with 2 mL microcentrifuge tubes for processing single samples The available plate seals are made of high quality polypropylene and help protect samples from contamination and evaporation especially during long term storage Applications • Protein and DNA desalting buffer exchange • Removal of dyes after protein labeling • Enzyme activity binding assays • Drug binding studies also see Rapid Equilbrium Dialysis RED Devices Each 96 well Microdialysis Plate comes pre loaded with twelve 8 dialysis device strips Each dialysis device strip can be removed from the plate and individual microdialysis devices easily separated allowing only the number of needed devices to be used and eliminating waste The sample chamber inside each microdialysis device is formed by two low binding regenerated cellulose membranes that are rated to retain proteins and other macromolecules 10 000 Daltons while allowing removal of buffer salts and contaminants 1000 Daltons Surface tension and the small distance between the membranes facilitate highly efficient sample recovery using standard laboratory pipettes Using the Microdialysis Plate sample dialysis can be efficiently performed in a standard 96 deep well plate with a minimal amount of buffer while still providing 95 removal of small molecules The assembled device is compatible with standard 96 well laboratory equipment and automated liquid handling systems making it an ideal option for high throughput applications Single microdialysis devices can be used independently in 2 mL microcentrifuge tube for processing single samples Related Products Pierce 96 well Microdialysis Plate 3 5K MWCO 96 well Deep well Plate 2 2 mL for Pierce 96 well Microdialysis Plate Seal for Pierce 96 well Microdialysis Plates
    Catalog Number:
    88260
    Price:
    None
    Applications:
    Dialysis, Desalting, and Concentration|Protein Biology|Protein Purification & Isolation
    Category:
    Instruments and Equipment
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    Structured Review

    Thermo Fisher 96 well microdialysis plate
    Thermo Scientific Pierce 96 well 10K MWCO Microdialysis Plates are automation compatible microplate dialysis devices for rapidly processing 1 to 96 samples with volumes 10 to 100 µL This kit comes with 12 cartridges of eight microdialysis devices in a 96 deep well plate Features of Microdialysis Plates • High yield greater than 90 protein recovery after dialysis • Rapid dialysis completed in 2 to 4 hours • Easy to use sample loading and retrieval can be done with a standard pipette • Flexible detachable 8 device strips scalable from 1 to 96 samples • Automation compatible plate format conforms to the SBS Microplate Standard Each sample processed with the Pierce 96 well Microdialysis Dialysis Plate is dialyzed inside a single removable dialysis device created by two regenerated cellulose membranes separated by 2mm The short diffusion distance and large surface area facilitates rapid dialysis with the removal of salts and small molecules often completed in 2 to 4 hours Sample recovery is performed using standard laboratory micropipettes Individual microdialysis devices can also be used with 2 mL microcentrifuge tubes for processing single samples The available plate seals are made of high quality polypropylene and help protect samples from contamination and evaporation especially during long term storage Applications • Protein and DNA desalting buffer exchange • Removal of dyes after protein labeling • Enzyme activity binding assays • Drug binding studies also see Rapid Equilbrium Dialysis RED Devices Each 96 well Microdialysis Plate comes pre loaded with twelve 8 dialysis device strips Each dialysis device strip can be removed from the plate and individual microdialysis devices easily separated allowing only the number of needed devices to be used and eliminating waste The sample chamber inside each microdialysis device is formed by two low binding regenerated cellulose membranes that are rated to retain proteins and other macromolecules 10 000 Daltons while allowing removal of buffer salts and contaminants 1000 Daltons Surface tension and the small distance between the membranes facilitate highly efficient sample recovery using standard laboratory pipettes Using the Microdialysis Plate sample dialysis can be efficiently performed in a standard 96 deep well plate with a minimal amount of buffer while still providing 95 removal of small molecules The assembled device is compatible with standard 96 well laboratory equipment and automated liquid handling systems making it an ideal option for high throughput applications Single microdialysis devices can be used independently in 2 mL microcentrifuge tube for processing single samples Related Products Pierce 96 well Microdialysis Plate 3 5K MWCO 96 well Deep well Plate 2 2 mL for Pierce 96 well Microdialysis Plate Seal for Pierce 96 well Microdialysis Plates
    https://www.bioz.com/result/96 well microdialysis plate/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    96 well microdialysis plate - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Diagnostic Assay:

    Article Title: The biochemistry and structural biology of cyanobactin biosynthetic enzymes
    Article Snippet: In the case of azoline-containing peptides, a diagnostic 255-nm shoulder in the UV spectrum aids in purification. .. For smaller-scale reactions, a 96-well microdialysis plate (Thermofisher, Pierce) can be used with similar conditions.

    Centrifugation:

    Article Title: SlZRT2 Encodes a ZIP Family Zn Transporter With Dual Localization in the Ectomycorrhizal Fungus Suillus luteus
    Article Snippet: Yeast cells were resuspended in 45 ml of ice-cold water and harvested by centrifugation. .. Crude extracts were dialyzed as described by with the PierceTM 96-well Microdialysis Plate (10K MWCO) (Thermo Fisher, Waltham, MA, United States).

    Synthesized:

    Article Title: Using DNA Devices to Track Anticancer Drug Activity
    Article Snippet: The membranes used in the chip to separate “nuclear” from “cytoplasmic” cofactors were the microdialysis strips associated with the Pierce 96-well microdialysis plate, 3.5K molecular weight cutoff (MWCO) from Thermo Scientific. .. The formamidopyrimidine DNA glycosylase was purchased from New England Biolabs and exchanged to the device buffer using a Pierce mini-dialysis kit (3.5k MWCO, Thermo Scientific). ß-Lap (3,4-dihydro-2,2-dimethyl-2 H -naphtho[1,2- b ]pyran-5,6-dione) was synthesized, confirmed by NMR, dissolved in DMSO at 50 μmol/L, and concentrations verified by spectrophotometry ( ).

    Nuclear Magnetic Resonance:

    Article Title: Using DNA Devices to Track Anticancer Drug Activity
    Article Snippet: The membranes used in the chip to separate “nuclear” from “cytoplasmic” cofactors were the microdialysis strips associated with the Pierce 96-well microdialysis plate, 3.5K molecular weight cutoff (MWCO) from Thermo Scientific. .. The formamidopyrimidine DNA glycosylase was purchased from New England Biolabs and exchanged to the device buffer using a Pierce mini-dialysis kit (3.5k MWCO, Thermo Scientific). ß-Lap (3,4-dihydro-2,2-dimethyl-2 H -naphtho[1,2- b ]pyran-5,6-dione) was synthesized, confirmed by NMR, dissolved in DMSO at 50 μmol/L, and concentrations verified by spectrophotometry ( ).

    Activity Assay:

    Article Title: The biochemistry and structural biology of cyanobactin biosynthetic enzymes
    Article Snippet: To maintain the activity of protease A, several different methods can be used to remove DTT: HPLC method. .. For smaller-scale reactions, a 96-well microdialysis plate (Thermofisher, Pierce) can be used with similar conditions.

    Article Title: Thiadiazolidinones: A New Class of Alanine Racemase Inhibitors with Antimicrobial Activity against Methicillin- Resistant S. aureus
    Article Snippet: In the second method, the enzyme-inhibitor mixture was placed in 96-well microdialysis plate with a molecular weight cutoff of 10000 (Pierce, Thermo Scientific, Rockford, IL), and dialyzed (10 mM Tricine, pH 8. .. Aliquots were withdrawn after 4 and 16 hours and Alr activity was determined.

    Article Title: SlZRT2 Encodes a ZIP Family Zn Transporter With Dual Localization in the Ectomycorrhizal Fungus Suillus luteus
    Article Snippet: Paragraph title: Trehalase Activity in Transformed Yeasts ... Crude extracts were dialyzed as described by with the PierceTM 96-well Microdialysis Plate (10K MWCO) (Thermo Fisher, Waltham, MA, United States).

    Expressing:

    Article Title: Development of a full-length human protein production pipeline
    Article Snippet: .. For each reaction, 4.4 μL of 1 μg/μL expression plasmid was mixed with the clarified IVT mixture, and 100 μL of the IVT reaction was transferred into a dialysis chamber of a 96-well microdialysis plate (Thermo-Pierce). .. The dialysis chambers were equilibrated with 20 min soaking in 1.8 mL, 30°C, 1X dialysis buffer (Thermo-Pierce) prior to adding the IVT reaction.

    Transformation Assay:

    Article Title: SlZRT2 Encodes a ZIP Family Zn Transporter With Dual Localization in the Ectomycorrhizal Fungus Suillus luteus
    Article Snippet: Paragraph title: Trehalase Activity in Transformed Yeasts ... Crude extracts were dialyzed as described by with the PierceTM 96-well Microdialysis Plate (10K MWCO) (Thermo Fisher, Waltham, MA, United States).

    High Performance Liquid Chromatography:

    Article Title: The biochemistry and structural biology of cyanobactin biosynthetic enzymes
    Article Snippet: To maintain the activity of protease A, several different methods can be used to remove DTT: HPLC method. .. For smaller-scale reactions, a 96-well microdialysis plate (Thermofisher, Pierce) can be used with similar conditions.

    Conjugation Assay:

    Article Title: Engineered, highly reactive substrates of microbial transglutaminase enable protein labeling within various secondary structure elements
    Article Snippet: Paragraph title: Fluorescent conjugation assays ... Excess, unreacted fluorophore was removed by dialysis using a Pierce™ 96‐well microdialysis plate with a 3.5 kDa MWCO (ThermoFisher).

    Flow Cytometry:

    Article Title: The biochemistry and structural biology of cyanobactin biosynthetic enzymes
    Article Snippet: For smaller-scale reactions, a 96-well microdialysis plate (Thermofisher, Pierce) can be used with similar conditions. .. After removing the storage buffer, the reaction mixture is passed through the column, and the flow-through is collected.

    Chromatography:

    Article Title: The biochemistry and structural biology of cyanobactin biosynthetic enzymes
    Article Snippet: For smaller-scale reactions, a 96-well microdialysis plate (Thermofisher, Pierce) can be used with similar conditions. .. Although the HPLC method is useful and provides a pure compound, recovery suffers due to the intrinsic limitations of chromatography.

    Polymerase Chain Reaction:

    Article Title: Development of a full-length human protein production pipeline
    Article Snippet: The reaction mixture was centrifuged at 10,000 g for 2 min, and 105.6 μL of supernatant was aliquoted per reaction into a 96-well polymerase chain reaction (PCR) plate. .. For each reaction, 4.4 μL of 1 μg/μL expression plasmid was mixed with the clarified IVT mixture, and 100 μL of the IVT reaction was transferred into a dialysis chamber of a 96-well microdialysis plate (Thermo-Pierce).

    Binding Assay:

    Article Title: Kinetic characterization of Vibrio cholerae ApbE: Substrate specificity and regulatory mechanisms
    Article Snippet: .. FAD binding assays To investigate the flavin binding capability ApbE, equilibrium binding experiments were performed using Pierce® 96-well Microdialysis Plate (Thermo scientific). .. Purified proteins were washed twice with reconstitution buffer.

    Molecular Weight:

    Article Title: Using DNA Devices to Track Anticancer Drug Activity
    Article Snippet: .. The membranes used in the chip to separate “nuclear” from “cytoplasmic” cofactors were the microdialysis strips associated with the Pierce 96-well microdialysis plate, 3.5K molecular weight cutoff (MWCO) from Thermo Scientific. .. The formamidopyrimidine DNA glycosylase was purchased from New England Biolabs and exchanged to the device buffer using a Pierce mini-dialysis kit (3.5k MWCO, Thermo Scientific). ß-Lap (3,4-dihydro-2,2-dimethyl-2 H -naphtho[1,2- b ]pyran-5,6-dione) was synthesized, confirmed by NMR, dissolved in DMSO at 50 μmol/L, and concentrations verified by spectrophotometry ( ).

    Article Title: Thiadiazolidinones: A New Class of Alanine Racemase Inhibitors with Antimicrobial Activity against Methicillin- Resistant S. aureus
    Article Snippet: .. In the second method, the enzyme-inhibitor mixture was placed in 96-well microdialysis plate with a molecular weight cutoff of 10000 (Pierce, Thermo Scientific, Rockford, IL), and dialyzed (10 mM Tricine, pH 8. ..

    Article Title: Following Anticancer Drug Activity in Cell Lysates with DNA Devices
    Article Snippet: .. The membranes used to separate nuclear lysates from cytoplasmic lysates were the microdialysis strips associated with the Pierce 96-well microdialysis plate, 3.5K molecular weight cutoff (MWCO) from Thermo Scientific. .. Our general approach for following β-lap activity in cell lysates is shown in .

    Transfection:

    Article Title: Assembly and Folding Properties of Cytosolic IgG Intrabodies
    Article Snippet: .. Aliquots of transfectant lysates were treated with 100 mM DTT for 30 min, then passed through a desalting column (ThermoFisher; cat# 89882) equilibrated with PBS, followed by micro-dialysis against PBS using a micro-dialyzer (ThermoFisher; cat# 88260) at 4 °C for 24 hours. ..

    Purification:

    Article Title: Kinetic characterization of Vibrio cholerae ApbE: Substrate specificity and regulatory mechanisms
    Article Snippet: FAD binding assays To investigate the flavin binding capability ApbE, equilibrium binding experiments were performed using Pierce® 96-well Microdialysis Plate (Thermo scientific). .. Purified proteins were washed twice with reconstitution buffer.

    Article Title: The biochemistry and structural biology of cyanobactin biosynthetic enzymes
    Article Snippet: In the case of azoline-containing peptides, a diagnostic 255-nm shoulder in the UV spectrum aids in purification. .. For smaller-scale reactions, a 96-well microdialysis plate (Thermofisher, Pierce) can be used with similar conditions.

    Article Title: Engineered, highly reactive substrates of microbial transglutaminase enable protein labeling within various secondary structure elements
    Article Snippet: Purified GB1 variants were quantified by measuring the A280 , using a molar extinction coefficient of 9970 M−1 cm−1 as calculated using ExPASy's ProtParam module. .. Excess, unreacted fluorophore was removed by dialysis using a Pierce™ 96‐well microdialysis plate with a 3.5 kDa MWCO (ThermoFisher).

    Chromatin Immunoprecipitation:

    Article Title: Using DNA Devices to Track Anticancer Drug Activity
    Article Snippet: .. The membranes used in the chip to separate “nuclear” from “cytoplasmic” cofactors were the microdialysis strips associated with the Pierce 96-well microdialysis plate, 3.5K molecular weight cutoff (MWCO) from Thermo Scientific. .. The formamidopyrimidine DNA glycosylase was purchased from New England Biolabs and exchanged to the device buffer using a Pierce mini-dialysis kit (3.5k MWCO, Thermo Scientific). ß-Lap (3,4-dihydro-2,2-dimethyl-2 H -naphtho[1,2- b ]pyran-5,6-dione) was synthesized, confirmed by NMR, dissolved in DMSO at 50 μmol/L, and concentrations verified by spectrophotometry ( ).

    Plasmid Preparation:

    Article Title: Development of a full-length human protein production pipeline
    Article Snippet: .. For each reaction, 4.4 μL of 1 μg/μL expression plasmid was mixed with the clarified IVT mixture, and 100 μL of the IVT reaction was transferred into a dialysis chamber of a 96-well microdialysis plate (Thermo-Pierce). .. The dialysis chambers were equilibrated with 20 min soaking in 1.8 mL, 30°C, 1X dialysis buffer (Thermo-Pierce) prior to adding the IVT reaction.

    Article Title: Improving Cell-Free Protein Synthesis through Genome Engineering of Escherichia coli Lacking Release Factor 1
    Article Snippet: Cell-free reactions (120 μL) were carried out in a microdialysis device (3.5 K MWCO) in a Pierce 96-well Microdialysis Plate (Thermo Fisher Scientific). .. [ ] The microdialysis device interfaces with 1500 μL of dialysis buffer that contains CFPS reagents as described in the “CFPS reaction” section without T7 RNA polymerase, plasmid, cell extract, o-tRNAopt , or pAcFRS.

    Recombinant:

    Article Title: Using DNA Devices to Track Anticancer Drug Activity
    Article Snippet: The membranes used in the chip to separate “nuclear” from “cytoplasmic” cofactors were the microdialysis strips associated with the Pierce 96-well microdialysis plate, 3.5K molecular weight cutoff (MWCO) from Thermo Scientific. .. Recombinant human NAD(P)H:quinone oxidoreductase 1 (NQO1) was purchased from Abcam (ab59663) and used as received.

    Incubation:

    Article Title: Engineered, highly reactive substrates of microbial transglutaminase enable protein labeling within various secondary structure elements
    Article Snippet: The final volume of each reaction was 150 μL and all were incubated at 37°C for 24 h. Aliquots of 50 μL were taken after 2 h, 6 h, and 24 h of reaction time, and quenched with the addition of 2 μL formic acid. .. Excess, unreacted fluorophore was removed by dialysis using a Pierce™ 96‐well microdialysis plate with a 3.5 kDa MWCO (ThermoFisher).

    Article Title: Thiadiazolidinones: A New Class of Alanine Racemase Inhibitors with Antimicrobial Activity against Methicillin- Resistant S. aureus
    Article Snippet: Aliquots were removed after 30 minutes, 6 hours and 16 hours of incubation and Alr assays were performed to determine the enzyme IC50 s as described above. .. In the second method, the enzyme-inhibitor mixture was placed in 96-well microdialysis plate with a molecular weight cutoff of 10000 (Pierce, Thermo Scientific, Rockford, IL), and dialyzed (10 mM Tricine, pH 8.

    Spectrophotometry:

    Article Title: Using DNA Devices to Track Anticancer Drug Activity
    Article Snippet: The membranes used in the chip to separate “nuclear” from “cytoplasmic” cofactors were the microdialysis strips associated with the Pierce 96-well microdialysis plate, 3.5K molecular weight cutoff (MWCO) from Thermo Scientific. .. The formamidopyrimidine DNA glycosylase was purchased from New England Biolabs and exchanged to the device buffer using a Pierce mini-dialysis kit (3.5k MWCO, Thermo Scientific). ß-Lap (3,4-dihydro-2,2-dimethyl-2 H -naphtho[1,2- b ]pyran-5,6-dione) was synthesized, confirmed by NMR, dissolved in DMSO at 50 μmol/L, and concentrations verified by spectrophotometry ( ).

    Concentration Assay:

    Article Title: Assembly and Folding Properties of Cytosolic IgG Intrabodies
    Article Snippet: Aliquots of transfectant lysates were treated with 100 mM DTT for 30 min, then passed through a desalting column (ThermoFisher; cat# 89882) equilibrated with PBS, followed by micro-dialysis against PBS using a micro-dialyzer (ThermoFisher; cat# 88260) at 4 °C for 24 hours. .. To validate the complete removal of DTT in the above column and dialysis steps, 100 mM DTT in PBS was passed through the desalting column then subjected to dialysis against PBS, and the concentration of DTT was determined using a free thiol detection kit (Abcam; cat# ab112158).

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  • 99
    Thermo Fisher pierce 96 well microdialysis plate
    Pierce 96 Well Microdialysis Plate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pierce 96 well microdialysis plate/product/Thermo Fisher
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    pierce 96 well microdialysis plate - by Bioz Stars, 2020-04
    99/100 stars
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