96 well deep plates  (Thermo Fisher)


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    Name:
    Deep Well Plate 96 well
    Description:
    96 well deep well plastic plates for nucleic acid purification on the KingFisher Flex system For veterinary use only Regulatory requirements vary by country products may not be available in your geographic area Biological hazard safetyWARNING BIOHAZARD Biological samples such as tissues body fluids infectious agents and blood of humans and other animals have the potential to transmit infectious diseases All work should be conducted in properly equipped facilities using the appropriate safety equipment for example physical containment devices Safety equipment also may include items for personal protection such as gloves coats gowns shoe covers boots respirators face shields safety glasses or goggles Individuals should be trained according to applicable regulatory and company institution requirements before working with potentially biohazardous materials Follow all applicable local state provincial and or national regulations The following references provide general guidelines when handling biological samples in laboratory environment U S Department of Health and Human Services Biosafety in Microbiological and Biomedical Laboratories BMBL 5th Edition HHS Publication No CDC 21 1112 Revised December 2009World Health Organization Laboratory Biosafety Manual 3rd Edition WHO CDS CSR LYO 2004 11
    Catalog Number:
    10045
    Price:
    None
    Applications:
    Animal Health|Industrial & Applied Science
    Category:
    Instruments and Equipment
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    Structured Review

    Thermo Fisher 96 well deep plates
    96 well deep well plastic plates for nucleic acid purification on the KingFisher Flex system For veterinary use only Regulatory requirements vary by country products may not be available in your geographic area Biological hazard safetyWARNING BIOHAZARD Biological samples such as tissues body fluids infectious agents and blood of humans and other animals have the potential to transmit infectious diseases All work should be conducted in properly equipped facilities using the appropriate safety equipment for example physical containment devices Safety equipment also may include items for personal protection such as gloves coats gowns shoe covers boots respirators face shields safety glasses or goggles Individuals should be trained according to applicable regulatory and company institution requirements before working with potentially biohazardous materials Follow all applicable local state provincial and or national regulations The following references provide general guidelines when handling biological samples in laboratory environment U S Department of Health and Human Services Biosafety in Microbiological and Biomedical Laboratories BMBL 5th Edition HHS Publication No CDC 21 1112 Revised December 2009World Health Organization Laboratory Biosafety Manual 3rd Edition WHO CDS CSR LYO 2004 11
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    Related Articles

    Clone Assay:

    Article Title: Selective Growth, In Vitro and In Vivo, of Individual T Cell Clones from Tumor-Infiltrating Lymphocytes Obtained from Patients with Melanoma
    Article Snippet: A total of 4 μ l of the eluted sample was used for cloning and transformation with the pcDNA3.1/V5-His TOPO TA Expression kit (Invitrogen Life Technologies). .. After overnight culture of bacterial plates at 37°C, at least 96 separate bacterial colonies were transferred into 96-well deep plates with 1 ml of Super Broth (BioSource International, Camarillo, CA) and 100 μ g/ml ampicillin for each well.

    Centrifugation:

    Article Title: Rapid Methods for High-Throughput Detection of Sulfoxides ▿
    Article Snippet: The growth was followed by centrifugation at 3,000 × g for 10 min at 25°C, using a Sigma 4K15 centrifuge (Sigma, Osterode, Germany). .. Four hundred microliters of the supernatant was transferred to a new polypropylene deep-well plate (ABgene; Thermo Fisher Scientific, Epson, United Kingdom), and 400 μl of ethyl acetate was added to each well (1:1 [vol/vol]) for the extraction of reactants.

    Amplification:

    Article Title: Selective Growth, In Vitro and In Vivo, of Individual T Cell Clones from Tumor-Infiltrating Lymphocytes Obtained from Patients with Melanoma
    Article Snippet: Amplified bands of ~700 bp were purified and eluted with 8 μ l of water using a Zymoclean Gel DNA Recovery kit (Zymo Research, Orange, CA). .. After overnight culture of bacterial plates at 37°C, at least 96 separate bacterial colonies were transferred into 96-well deep plates with 1 ml of Super Broth (BioSource International, Camarillo, CA) and 100 μ g/ml ampicillin for each well.

    Mass Spectrometry:

    Article Title:
    Article Snippet: Samples and standards were prepared in plastic tubes (Bioanalytical Systems) that were loaded into a 96-deep-well plate and then heat sealed (Thermo Scientific Easy Peel, Waltham, MA) prior to placing them onto the Acquity sample organizer for analysis. .. A Waters Acquity high-pressure liquid chromatography system (Milford, MA) equipped with a Sunshell RP-Aqua 2.1 × 100-mm, 2.6-µ m particle column was used to isolate ACh prior to detection using a Waters Quattro Premier XE triple-quadrupole mass spectrometer operating in the mass spectrometry/mass spectrometry mode.

    Cytometry:

    Article Title: The Impact of Different Sources of Fluctuations on Mutual Information in Biochemical Networks
    Article Snippet: Paragraph title: Growth conditions and flow cytometry ... Using a deep-well 96-well plate (Thermo), estradiol concentrations decreased from columns one to eleven in logarithmically spaced points according to the following equation: 100(2/3)c −1 nM where c denotes the column number.

    Electrophoresis:

    Article Title: Selective Growth, In Vitro and In Vivo, of Individual T Cell Clones from Tumor-Infiltrating Lymphocytes Obtained from Patients with Melanoma
    Article Snippet: A total of 20 μ l of PCR product for each sample was separated by electrophoresis using 2% agarose E-Gel (Invitrogen Life Technologies, Carlsbad, CA). .. After overnight culture of bacterial plates at 37°C, at least 96 separate bacterial colonies were transferred into 96-well deep plates with 1 ml of Super Broth (BioSource International, Camarillo, CA) and 100 μ g/ml ampicillin for each well.

    Ex Vivo:

    Article Title:
    Article Snippet: Brains were quickly dissected from the skull, flash frozen on powdered dry ice, and stored at −20°C until used in ex vivo receptor occupancy experiments. .. Samples and standards were prepared in plastic tubes (Bioanalytical Systems) that were loaded into a 96-deep-well plate and then heat sealed (Thermo Scientific Easy Peel, Waltham, MA) prior to placing them onto the Acquity sample organizer for analysis.

    Incubation:

    Article Title: Diazepam is not a direct allosteric modulator of α1‐adrenoceptors, but modulates receptor signaling by inhibiting phosphodiesterase‐4, et al. Diazepam is not a direct allosteric modulator of α1‐adrenoceptors, but modulates receptor signaling by inhibiting phosphodiesterase‐4
    Article Snippet: Briefly, 2 nmol of purified receptor, with C‐terminal mCherry‐Avi tag fusion, were incubated with 100 μL Dynabeads® MyOne Streptavidin T1 paramagnetic beads (Life Technologies, Carlsbad, CA, USA) in 10 mL binding buffer (1× PBS, 0.05% n ‐dodecyl β‐D‐maltopyranoside [DDM], 10 mmol/L EDTA), at 4°C for 1.5 hours. .. The receptor‐coated beads were washed in binding buffer, and then aliquoted into a KingFisher 96‐DeepWell™plate (Thermo Fisher Scientific, Waltham, MA, USA) at approximately 20 pmol of receptor per well.

    Article Title: Native Plasmid-Encoded Mercury Resistance Genes Are Functional and Demonstrate Natural Transformation in Environmental Bacterial Isolates
    Article Snippet: About 250 μl of each culture was added to individual wells of a 96-well deep-well plate (Costar, Thermo Fisher Scientific). .. The plate was incubated at 30°C without shaking to enable natural transformation.

    Article Title: Rational modification of vanillin derivatives to stereospecifically destabilize sickle hemoglobin polymer formation
    Article Snippet: .. In a 96-well 1.2 ml polypropylene deep-well plate (Thermo Scientific), 600 µl of whole blood from normal healthy donors (adjusted to 30% final hematocrit) was supplemented with each compound to a final concentration of 2 m M and incubated at 37°C for 24 h with shaking (100 rev min−1 ). ..

    Article Title: Rapid Methods for High-Throughput Detection of Sulfoxides ▿
    Article Snippet: Four hundred microliters of the supernatant was transferred to a new polypropylene deep-well plate (ABgene; Thermo Fisher Scientific, Epson, United Kingdom), and 400 μl of ethyl acetate was added to each well (1:1 [vol/vol]) for the extraction of reactants. .. TFAA (10 μl from a 0.5 M stock in acetone) and sodium iodide (NaI) (100 μl from a 0.5 M stock in acetone) were added, followed by vigorous pipetting and a 2-min incubation at room temperature.

    Article Title: The Lineage Determining Factor GRHL2 Collaborates with FOXA1 to Establish a Targetable Pathway in Endocrine Therapy-Resistant Breast Cancer
    Article Snippet: .. Sheared, diluted chromatin was incubated with respective antibodies overnight in a deep 96-well plate at 4○ C and then captured on protein A magnetic beads (Invitrogen, DynaI). .. After 45 min of incubation with the beads the immunoprecipitates were washed on a 96-well microplate in 150 uL volumes of the following solutions.

    Expressing:

    Article Title: Selective Growth, In Vitro and In Vivo, of Individual T Cell Clones from Tumor-Infiltrating Lymphocytes Obtained from Patients with Melanoma
    Article Snippet: Paragraph title: 5′ RACE analysis of TRBV gene expression ... After overnight culture of bacterial plates at 37°C, at least 96 separate bacterial colonies were transferred into 96-well deep plates with 1 ml of Super Broth (BioSource International, Camarillo, CA) and 100 μ g/ml ampicillin for each well.

    Article Title: Diazepam is not a direct allosteric modulator of α1‐adrenoceptors, but modulates receptor signaling by inhibiting phosphodiesterase‐4, et al. Diazepam is not a direct allosteric modulator of α1‐adrenoceptors, but modulates receptor signaling by inhibiting phosphodiesterase‐4
    Article Snippet: Paragraph title: Protein expression, purification, and binding assays ... The receptor‐coated beads were washed in binding buffer, and then aliquoted into a KingFisher 96‐DeepWell™plate (Thermo Fisher Scientific, Waltham, MA, USA) at approximately 20 pmol of receptor per well.

    Modification:

    Article Title: Rational modification of vanillin derivatives to stereospecifically destabilize sickle hemoglobin polymer formation
    Article Snippet: We investigated the compounds in vitro for their time- and concentration-dependent effect on Hb-adduct formation (Hb modification). .. In a 96-well 1.2 ml polypropylene deep-well plate (Thermo Scientific), 600 µl of whole blood from normal healthy donors (adjusted to 30% final hematocrit) was supplemented with each compound to a final concentration of 2 m M and incubated at 37°C for 24 h with shaking (100 rev min−1 ).

    Article Title: BEAMing and Droplet Digital PCR Analysis of Mutant IDH1 mRNA in Glioma Patient Serum and Cerebrospinal Fluid Extracellular Vesicles
    Article Snippet: We set up 150 μl PCR reactions containing 10 μl 20 pmol/l template DNA, 2X TITANIUM Taq DNA Polymerase (Clontech, Mountain View, CA), 1X TITANIUM Taq buffer (Clontech), 1 mmol/l dNTP mix, 8.3 mmol/l MgCl2 (Invitrogen), 0.05 μmol/l universal forward primer (5′-tcccgcgaaattaatacgac-3′), 8 μmol/l nested reverse primer (5′-AATCAGTTGCTCTGTATTGATCC-3′), and 6 × 107 magnetic streptavidin beads (MyOne, Invitrogen) coated with a modified universal forward primer (5′-dual biotion-T-Spacer18-tcccgcgaaattaatacgac-3′). .. The aqueous reaction mixture was then combined with 600 μl of an oil/emulsifier mixture of 7% w/v ABIL WE09 (Degussa, Essen, GE), 20% v/v M3516 mineral oil (Sigma-Aldrich, St Louis, MO) and 73% v/v Tegosoft DEC (Degussa), and one 5 mm steel bead (Qiagen) in one well of a 96-deep-well plate (Abgene, Lafayette, CO).

    Transformation Assay:

    Article Title: Selective Growth, In Vitro and In Vivo, of Individual T Cell Clones from Tumor-Infiltrating Lymphocytes Obtained from Patients with Melanoma
    Article Snippet: A total of 4 μ l of the eluted sample was used for cloning and transformation with the pcDNA3.1/V5-His TOPO TA Expression kit (Invitrogen Life Technologies). .. After overnight culture of bacterial plates at 37°C, at least 96 separate bacterial colonies were transferred into 96-well deep plates with 1 ml of Super Broth (BioSource International, Camarillo, CA) and 100 μ g/ml ampicillin for each well.

    High Performance Liquid Chromatography:

    Article Title:
    Article Snippet: Samples and standards were prepared in plastic tubes (Bioanalytical Systems) that were loaded into a 96-deep-well plate and then heat sealed (Thermo Scientific Easy Peel, Waltham, MA) prior to placing them onto the Acquity sample organizer for analysis. .. A Waters Acquity high-pressure liquid chromatography system (Milford, MA) equipped with a Sunshell RP-Aqua 2.1 × 100-mm, 2.6-µ m particle column was used to isolate ACh prior to detection using a Waters Quattro Premier XE triple-quadrupole mass spectrometer operating in the mass spectrometry/mass spectrometry mode.

    Flow Cytometry:

    Article Title: The Impact of Different Sources of Fluctuations on Mutual Information in Biochemical Networks
    Article Snippet: Paragraph title: Growth conditions and flow cytometry ... Using a deep-well 96-well plate (Thermo), estradiol concentrations decreased from columns one to eleven in logarithmically spaced points according to the following equation: 100(2/3)c −1 nM where c denotes the column number.

    Chromatography:

    Article Title:
    Article Snippet: Samples and standards were prepared in plastic tubes (Bioanalytical Systems) that were loaded into a 96-deep-well plate and then heat sealed (Thermo Scientific Easy Peel, Waltham, MA) prior to placing them onto the Acquity sample organizer for analysis. .. A full loop injection using a 5 µ L loop with a 4× overfill, requiring a total sample volume of 20 µ L, was used to deliver samples onto the liquid chromatography/mass spectrometry/mass spectrometry system.

    Immunoprecipitation:

    Article Title: Early inflammatory response in epithelial ovarian tumor cyst fluids
    Article Snippet: Paragraph title: Immunoprecipitation–mass spectrometry ... From each sample, 400-μ L cyst fluid was added to 300 μ L 150 mmol/L TrisHCl, 0.15 mol/L NaCl, and 0.1% Tween 20 containing protease inhibitor cocktail (Roche, Branford, CT) with 50 μ L 50% v/v immobilized Antibody Mix I agarose beads (anti-human IL-8, anti-human IL-1β , anti-human regulated on activation normal T cell expressed and secreted, anti-human MCP-1, anti-human MIP1α , anti-human GROα , anti-human SDF1) in an perfluorodecyltrichlorosilane (FDTS)-modified fritted 96-deepwell filter plate (Nunc, Thermo Fisher Scientific Inc., Waltham, MA).

    Protease Inhibitor:

    Article Title: Early inflammatory response in epithelial ovarian tumor cyst fluids
    Article Snippet: .. From each sample, 400-μ L cyst fluid was added to 300 μ L 150 mmol/L TrisHCl, 0.15 mol/L NaCl, and 0.1% Tween 20 containing protease inhibitor cocktail (Roche, Branford, CT) with 50 μ L 50% v/v immobilized Antibody Mix I agarose beads (anti-human IL-8, anti-human IL-1β , anti-human regulated on activation normal T cell expressed and secreted, anti-human MCP-1, anti-human MIP1α , anti-human GROα , anti-human SDF1) in an perfluorodecyltrichlorosilane (FDTS)-modified fritted 96-deepwell filter plate (Nunc, Thermo Fisher Scientific Inc., Waltham, MA). ..

    Article Title: The Lineage Determining Factor GRHL2 Collaborates with FOXA1 to Establish a Targetable Pathway in Endocrine Therapy-Resistant Breast Cancer
    Article Snippet: Cell pellets were thawed on ice, and then resuspended in Lysis Buffer containing 1% SDS, 1 mM EDTA, 10 mM Tris-HCl, pH 8.0, protease inhibitor (Roche, Complete protease inhibitor tablets 11697498001). .. Sheared, diluted chromatin was incubated with respective antibodies overnight in a deep 96-well plate at 4○ C and then captured on protein A magnetic beads (Invitrogen, DynaI).

    Transferring:

    Article Title: Rational modification of vanillin derivatives to stereospecifically destabilize sickle hemoglobin polymer formation
    Article Snippet: In a 96-well 1.2 ml polypropylene deep-well plate (Thermo Scientific), 600 µl of whole blood from normal healthy donors (adjusted to 30% final hematocrit) was supplemented with each compound to a final concentration of 2 m M and incubated at 37°C for 24 h with shaking (100 rev min−1 ). .. At defined (0.5, 1, 2, 4 and 12 h) time intervals, 75 µl aliquot samples of blood were simultaneously removed from individual wells using a multichannel pipette and immediately added to respective tubes containing 75 µl sodium cyanoborohydride and borohydride mixture [1:1( v : v ) 50 m M stock] to terminate the Schiff-base reaction, fix the Schiff-base adducts and reduce the free reactive aldehyde.

    other:

    Article Title: High-throughput polyribosome fractionation
    Article Snippet: 96-well Deep Well plates (Nalge Nunc International, Cat. No. 270141) were used for sucrose gradients.

    Sequencing:

    Article Title: Selective Growth, In Vitro and In Vivo, of Individual T Cell Clones from Tumor-Infiltrating Lymphocytes Obtained from Patients with Melanoma
    Article Snippet: 3′ TCRBCN primer anneals the C region of the TCR β -chain, and its sequence is 5′-CGA GGT AAA GCC ACA GTC T-3′. .. After overnight culture of bacterial plates at 37°C, at least 96 separate bacterial colonies were transferred into 96-well deep plates with 1 ml of Super Broth (BioSource International, Camarillo, CA) and 100 μ g/ml ampicillin for each well.

    Sonication:

    Article Title: The Lineage Determining Factor GRHL2 Collaborates with FOXA1 to Establish a Targetable Pathway in Endocrine Therapy-Resistant Breast Cancer
    Article Snippet: Cell lysates were sonicated using the Covaris E210 (for FOXA1 H3K4Me2, and H3K27Ac ChIP-seq samples) or E220 (for GRHL2 ChIP-seq samples) instrument according to the manufacturers’ instructions. .. Sheared, diluted chromatin was incubated with respective antibodies overnight in a deep 96-well plate at 4○ C and then captured on protein A magnetic beads (Invitrogen, DynaI).

    Injection:

    Article Title:
    Article Snippet: Samples and standards were prepared in plastic tubes (Bioanalytical Systems) that were loaded into a 96-deep-well plate and then heat sealed (Thermo Scientific Easy Peel, Waltham, MA) prior to placing them onto the Acquity sample organizer for analysis. .. A full loop injection using a 5 µ L loop with a 4× overfill, requiring a total sample volume of 20 µ L, was used to deliver samples onto the liquid chromatography/mass spectrometry/mass spectrometry system.

    Binding Assay:

    Article Title: Diazepam is not a direct allosteric modulator of α1‐adrenoceptors, but modulates receptor signaling by inhibiting phosphodiesterase‐4, et al. Diazepam is not a direct allosteric modulator of α1‐adrenoceptors, but modulates receptor signaling by inhibiting phosphodiesterase‐4
    Article Snippet: .. The receptor‐coated beads were washed in binding buffer, and then aliquoted into a KingFisher 96‐DeepWell™plate (Thermo Fisher Scientific, Waltham, MA, USA) at approximately 20 pmol of receptor per well. .. A Kingfisher 96 magnetic particle processor was then used to transfer the beads into plates containing QAPB and various competitors, which was incubated for 2 hours at 22°C with gentle mixing.

    ChIP-sequencing:

    Article Title: The Lineage Determining Factor GRHL2 Collaborates with FOXA1 to Establish a Targetable Pathway in Endocrine Therapy-Resistant Breast Cancer
    Article Snippet: Paragraph title: ChIP-Seq ... Sheared, diluted chromatin was incubated with respective antibodies overnight in a deep 96-well plate at 4○ C and then captured on protein A magnetic beads (Invitrogen, DynaI).

    In Vivo:

    Article Title:
    Article Snippet: Paragraph title: In Vivo Microdialysis Experiments ... Samples and standards were prepared in plastic tubes (Bioanalytical Systems) that were loaded into a 96-deep-well plate and then heat sealed (Thermo Scientific Easy Peel, Waltham, MA) prior to placing them onto the Acquity sample organizer for analysis.

    Fluorescence:

    Article Title: Diazepam is not a direct allosteric modulator of α1‐adrenoceptors, but modulates receptor signaling by inhibiting phosphodiesterase‐4, et al. Diazepam is not a direct allosteric modulator of α1‐adrenoceptors, but modulates receptor signaling by inhibiting phosphodiesterase‐4
    Article Snippet: The receptor‐coated beads were washed in binding buffer, and then aliquoted into a KingFisher 96‐DeepWell™plate (Thermo Fisher Scientific, Waltham, MA, USA) at approximately 20 pmol of receptor per well. .. QAPB fluorescence was measured using 485/12 nm excitation and 520/10 nm emission filters, while mCherry fluorescence was measured using 544 nm excitation and 590/10 nm emission filters in an Omega POLARstar plate reader (BMG Labtech, Ortenberg, Germany).

    Article Title: The Impact of Different Sources of Fluctuations on Mutual Information in Biochemical Networks
    Article Snippet: Using a deep-well 96-well plate (Thermo), estradiol concentrations decreased from columns one to eleven in logarithmically spaced points according to the following equation: 100(2/3)c −1 nM where c denotes the column number. .. Fluorescence measurements were performed on a LSRII analyzer (BD Biosciences).

    Article Title: Rapid Methods for High-Throughput Detection of Sulfoxides ▿
    Article Snippet: Bacterial growth was performed as described for the fluorescence test. .. Four hundred microliters of the supernatant was transferred to a new polypropylene deep-well plate (ABgene; Thermo Fisher Scientific, Epson, United Kingdom), and 400 μl of ethyl acetate was added to each well (1:1 [vol/vol]) for the extraction of reactants.

    Magnetic Beads:

    Article Title: The Lineage Determining Factor GRHL2 Collaborates with FOXA1 to Establish a Targetable Pathway in Endocrine Therapy-Resistant Breast Cancer
    Article Snippet: .. Sheared, diluted chromatin was incubated with respective antibodies overnight in a deep 96-well plate at 4○ C and then captured on protein A magnetic beads (Invitrogen, DynaI). .. After 45 min of incubation with the beads the immunoprecipitates were washed on a 96-well microplate in 150 uL volumes of the following solutions.

    Isolation:

    Article Title: Native Plasmid-Encoded Mercury Resistance Genes Are Functional and Demonstrate Natural Transformation in Environmental Bacterial Isolates
    Article Snippet: The groundwater source, isolation conditions, GenBank accession numbers, taxonomic assignments, and 16S rRNA sequences of the 58 strains are provided in . .. The arrayed isolates were recovered in a 96-well deep-well plate (Costar, Thermo Fisher Scientific, Waltham, MA, USA) by addition of 1 ml of appropriate growth (either LB or R2A) medium.

    Purification:

    Article Title: Selective Growth, In Vitro and In Vivo, of Individual T Cell Clones from Tumor-Infiltrating Lymphocytes Obtained from Patients with Melanoma
    Article Snippet: Amplified bands of ~700 bp were purified and eluted with 8 μ l of water using a Zymoclean Gel DNA Recovery kit (Zymo Research, Orange, CA). .. After overnight culture of bacterial plates at 37°C, at least 96 separate bacterial colonies were transferred into 96-well deep plates with 1 ml of Super Broth (BioSource International, Camarillo, CA) and 100 μ g/ml ampicillin for each well.

    Article Title: Diazepam is not a direct allosteric modulator of α1‐adrenoceptors, but modulates receptor signaling by inhibiting phosphodiesterase‐4, et al. Diazepam is not a direct allosteric modulator of α1‐adrenoceptors, but modulates receptor signaling by inhibiting phosphodiesterase‐4
    Article Snippet: Paragraph title: Protein expression, purification, and binding assays ... The receptor‐coated beads were washed in binding buffer, and then aliquoted into a KingFisher 96‐DeepWell™plate (Thermo Fisher Scientific, Waltham, MA, USA) at approximately 20 pmol of receptor per well.

    Article Title: BEAMing and Droplet Digital PCR Analysis of Mutant IDH1 mRNA in Glioma Patient Serum and Cerebrospinal Fluid Extracellular Vesicles
    Article Snippet: The PCR products for each sample were then pooled and purified using a QIAquick PCR purification column (Qiagen) and the concentration determined using a 2100 Bioanalyzer with a DNA 1000 LabChip kit (Agilent). .. The aqueous reaction mixture was then combined with 600 μl of an oil/emulsifier mixture of 7% w/v ABIL WE09 (Degussa, Essen, GE), 20% v/v M3516 mineral oil (Sigma-Aldrich, St Louis, MO) and 73% v/v Tegosoft DEC (Degussa), and one 5 mm steel bead (Qiagen) in one well of a 96-deep-well plate (Abgene, Lafayette, CO).

    Polymerase Chain Reaction:

    Article Title: Selective Growth, In Vitro and In Vivo, of Individual T Cell Clones from Tumor-Infiltrating Lymphocytes Obtained from Patients with Melanoma
    Article Snippet: A total of 20 μ l of PCR product for each sample was separated by electrophoresis using 2% agarose E-Gel (Invitrogen Life Technologies, Carlsbad, CA). .. After overnight culture of bacterial plates at 37°C, at least 96 separate bacterial colonies were transferred into 96-well deep plates with 1 ml of Super Broth (BioSource International, Camarillo, CA) and 100 μ g/ml ampicillin for each well.

    Article Title: BEAMing and Droplet Digital PCR Analysis of Mutant IDH1 mRNA in Glioma Patient Serum and Cerebrospinal Fluid Extracellular Vesicles
    Article Snippet: We set up 150 μl PCR reactions containing 10 μl 20 pmol/l template DNA, 2X TITANIUM Taq DNA Polymerase (Clontech, Mountain View, CA), 1X TITANIUM Taq buffer (Clontech), 1 mmol/l dNTP mix, 8.3 mmol/l MgCl2 (Invitrogen), 0.05 μmol/l universal forward primer (5′-tcccgcgaaattaatacgac-3′), 8 μmol/l nested reverse primer (5′-AATCAGTTGCTCTGTATTGATCC-3′), and 6 × 107 magnetic streptavidin beads (MyOne, Invitrogen) coated with a modified universal forward primer (5′-dual biotion-T-Spacer18-tcccgcgaaattaatacgac-3′). .. The aqueous reaction mixture was then combined with 600 μl of an oil/emulsifier mixture of 7% w/v ABIL WE09 (Degussa, Essen, GE), 20% v/v M3516 mineral oil (Sigma-Aldrich, St Louis, MO) and 73% v/v Tegosoft DEC (Degussa), and one 5 mm steel bead (Qiagen) in one well of a 96-deep-well plate (Abgene, Lafayette, CO).

    Concentration Assay:

    Article Title: Rational modification of vanillin derivatives to stereospecifically destabilize sickle hemoglobin polymer formation
    Article Snippet: .. In a 96-well 1.2 ml polypropylene deep-well plate (Thermo Scientific), 600 µl of whole blood from normal healthy donors (adjusted to 30% final hematocrit) was supplemented with each compound to a final concentration of 2 m M and incubated at 37°C for 24 h with shaking (100 rev min−1 ). ..

    Article Title: BEAMing and Droplet Digital PCR Analysis of Mutant IDH1 mRNA in Glioma Patient Serum and Cerebrospinal Fluid Extracellular Vesicles
    Article Snippet: The PCR products for each sample were then pooled and purified using a QIAquick PCR purification column (Qiagen) and the concentration determined using a 2100 Bioanalyzer with a DNA 1000 LabChip kit (Agilent). .. The aqueous reaction mixture was then combined with 600 μl of an oil/emulsifier mixture of 7% w/v ABIL WE09 (Degussa, Essen, GE), 20% v/v M3516 mineral oil (Sigma-Aldrich, St Louis, MO) and 73% v/v Tegosoft DEC (Degussa), and one 5 mm steel bead (Qiagen) in one well of a 96-deep-well plate (Abgene, Lafayette, CO).

    Article Title: Rapid Methods for High-Throughput Detection of Sulfoxides ▿
    Article Snippet: The substrate, methyl phenyl sulfide, was added to 500 μl of bacterial suspension to a final concentration of 1 mM (from a 50 mM stock solution in ethanol), and the biotransformation was carried out for 5 h with shaking at 750 rpm, using a Vibramax 100 shaker (Heidolph, Nurenberg, Germany), at 25°C. .. Four hundred microliters of the supernatant was transferred to a new polypropylene deep-well plate (ABgene; Thermo Fisher Scientific, Epson, United Kingdom), and 400 μl of ethyl acetate was added to each well (1:1 [vol/vol]) for the extraction of reactants.

    Chromatin Immunoprecipitation:

    Article Title: The Lineage Determining Factor GRHL2 Collaborates with FOXA1 to Establish a Targetable Pathway in Endocrine Therapy-Resistant Breast Cancer
    Article Snippet: Sheared, diluted chromatin was incubated with respective antibodies overnight in a deep 96-well plate at 4○ C and then captured on protein A magnetic beads (Invitrogen, DynaI). .. For FoxA1, H3K4me2, and H3K27Ac ChIP, beads were washed a total of 6 times: 4 times in RIPA buffer containing 500 mM LiCl (50 mM HEPES, 1 mM EDTA, 0.7% Na Deoxycholate, 1% NP-40, 500 mM LiCl), and twice with TE buffer (20 mM Tris pH 8.0, 2mM EDTA).

    Plasmid Preparation:

    Article Title: Selective Growth, In Vitro and In Vivo, of Individual T Cell Clones from Tumor-Infiltrating Lymphocytes Obtained from Patients with Melanoma
    Article Snippet: After overnight culture of bacterial plates at 37°C, at least 96 separate bacterial colonies were transferred into 96-well deep plates with 1 ml of Super Broth (BioSource International, Camarillo, CA) and 100 μ g/ml ampicillin for each well. .. After overnight culture at 37°C under 300 rpm shaking, plasmid DNA samples were prepared from 96-well deep plates using a QIAprep 96 Turbo Miniprep kit (Qiagen).

    In Vitro:

    Article Title: Rational modification of vanillin derivatives to stereospecifically destabilize sickle hemoglobin polymer formation
    Article Snippet: Paragraph title: 2.4. In vitro time-dependent Hb-adduct formation   ... In a 96-well 1.2 ml polypropylene deep-well plate (Thermo Scientific), 600 µl of whole blood from normal healthy donors (adjusted to 30% final hematocrit) was supplemented with each compound to a final concentration of 2 m M and incubated at 37°C for 24 h with shaking (100 rev min−1 ).

    Activation Assay:

    Article Title: Early inflammatory response in epithelial ovarian tumor cyst fluids
    Article Snippet: .. From each sample, 400-μ L cyst fluid was added to 300 μ L 150 mmol/L TrisHCl, 0.15 mol/L NaCl, and 0.1% Tween 20 containing protease inhibitor cocktail (Roche, Branford, CT) with 50 μ L 50% v/v immobilized Antibody Mix I agarose beads (anti-human IL-8, anti-human IL-1β , anti-human regulated on activation normal T cell expressed and secreted, anti-human MCP-1, anti-human MIP1α , anti-human GROα , anti-human SDF1) in an perfluorodecyltrichlorosilane (FDTS)-modified fritted 96-deepwell filter plate (Nunc, Thermo Fisher Scientific Inc., Waltham, MA). ..

    Fractionation:

    Article Title: Early inflammatory response in epithelial ovarian tumor cyst fluids
    Article Snippet: We performed high-throughput sample fractionation by tandem antibody libraries bead, in combination with surface-enhanced laser desorption/ionization time of flight (SELDI). .. From each sample, 400-μ L cyst fluid was added to 300 μ L 150 mmol/L TrisHCl, 0.15 mol/L NaCl, and 0.1% Tween 20 containing protease inhibitor cocktail (Roche, Branford, CT) with 50 μ L 50% v/v immobilized Antibody Mix I agarose beads (anti-human IL-8, anti-human IL-1β , anti-human regulated on activation normal T cell expressed and secreted, anti-human MCP-1, anti-human MIP1α , anti-human GROα , anti-human SDF1) in an perfluorodecyltrichlorosilane (FDTS)-modified fritted 96-deepwell filter plate (Nunc, Thermo Fisher Scientific Inc., Waltham, MA).

    High Throughput Screening Assay:

    Article Title: Native Plasmid-Encoded Mercury Resistance Genes Are Functional and Demonstrate Natural Transformation in Environmental Bacterial Isolates
    Article Snippet: Paragraph title: HGT via high-throughput screen. ... The arrayed isolates were recovered in a 96-well deep-well plate (Costar, Thermo Fisher Scientific, Waltham, MA, USA) by addition of 1 ml of appropriate growth (either LB or R2A) medium.

    Article Title: Early inflammatory response in epithelial ovarian tumor cyst fluids
    Article Snippet: We performed high-throughput sample fractionation by tandem antibody libraries bead, in combination with surface-enhanced laser desorption/ionization time of flight (SELDI). .. From each sample, 400-μ L cyst fluid was added to 300 μ L 150 mmol/L TrisHCl, 0.15 mol/L NaCl, and 0.1% Tween 20 containing protease inhibitor cocktail (Roche, Branford, CT) with 50 μ L 50% v/v immobilized Antibody Mix I agarose beads (anti-human IL-8, anti-human IL-1β , anti-human regulated on activation normal T cell expressed and secreted, anti-human MCP-1, anti-human MIP1α , anti-human GROα , anti-human SDF1) in an perfluorodecyltrichlorosilane (FDTS)-modified fritted 96-deepwell filter plate (Nunc, Thermo Fisher Scientific Inc., Waltham, MA).

    Lysis:

    Article Title: The Lineage Determining Factor GRHL2 Collaborates with FOXA1 to Establish a Targetable Pathway in Endocrine Therapy-Resistant Breast Cancer
    Article Snippet: Cell pellets were thawed on ice, and then resuspended in Lysis Buffer containing 1% SDS, 1 mM EDTA, 10 mM Tris-HCl, pH 8.0, protease inhibitor (Roche, Complete protease inhibitor tablets 11697498001). .. Sheared, diluted chromatin was incubated with respective antibodies overnight in a deep 96-well plate at 4○ C and then captured on protein A magnetic beads (Invitrogen, DynaI).

    Variant Assay:

    Article Title: Diazepam is not a direct allosteric modulator of α1‐adrenoceptors, but modulates receptor signaling by inhibiting phosphodiesterase‐4, et al. Diazepam is not a direct allosteric modulator of α1‐adrenoceptors, but modulates receptor signaling by inhibiting phosphodiesterase‐4
    Article Snippet: 2.2 Protein expression, purification, and binding assays α1A ‐AR variant A4 and α1B ‐AR variant #15 were expressed in Escherichia coli and purified as described previously. .. The receptor‐coated beads were washed in binding buffer, and then aliquoted into a KingFisher 96‐DeepWell™plate (Thermo Fisher Scientific, Waltham, MA, USA) at approximately 20 pmol of receptor per well.

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    Thermo Fisher 96 deep well plate
    96 Deep Well Plate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher nunc deepwell plates
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    Thermo Fisher 96 well plate individual v bottom
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