Journal: Virology Journal

Article Title: Efficacy of phage vB_Ps_ZCPS13 in controlling Pan-drug-resistant Pseudomonas aeruginosa from urinary tract infections (UTIs) and eradicating biofilms from urinary catheters

doi: 10.1186/s12985-025-02848-x

Figure Lengend Snippet: Antibiotic sensitivity profile for 20 UTI P. aeruginosa isolates over 10 antibiotics (sensitivity is indicated in green, intermediate in yellow, and resistance in pink) with their MAR index values

Article Snippet: The reference strain Pseudomonas aeruginosa NCTC 12,903 / ATCC 27,853 was used as a quality control in the susceptibility test.

Techniques:

Journal: Virology Journal

Article Title: Efficacy of phage vB_Ps_ZCPS13 in controlling Pan-drug-resistant Pseudomonas aeruginosa from urinary tract infections (UTIs) and eradicating biofilms from urinary catheters

doi: 10.1186/s12985-025-02848-x

Figure Lengend Snippet: Heatmap of host range and EOP for different P. aeruginosa isolates tested against phage vB_Ps_ZCPS13. ( a ) The host range results represent light green for lysed bacteria and red for resistant bacteria. ( b ) The EOP values are represented as green (≥ 1), light red (≥ 0.001 and < 1), and dark red (< 0.001)

Article Snippet: The reference strain Pseudomonas aeruginosa NCTC 12,903 / ATCC 27,853 was used as a quality control in the susceptibility test.

Techniques: Bacteria

Journal: Virology Journal

Article Title: Efficacy of phage vB_Ps_ZCPS13 in controlling Pan-drug-resistant Pseudomonas aeruginosa from urinary tract infections (UTIs) and eradicating biofilms from urinary catheters

doi: 10.1186/s12985-025-02848-x

Figure Lengend Snippet: Representative SEM images of silicone urinary catheter surfaces treated with phage vB_Ps_ZCPS13. (a1 and b1) Untreated control for biofilm inhibition and biofilm clearance, respectively, (a2 and b2) phage-treated after 48 h for biofilm inhibition and biofilm clearance, respectively at magnification of 5000x. The relative number of CFU of P. aeruginosa biofilms formed in treated and untreated catheters for biofilm inhibition (c1) and biofilm clearance (c2). Statistically significant difference marked by asterisks, where *** indicates P < 0.0001

Article Snippet: The reference strain Pseudomonas aeruginosa NCTC 12,903 / ATCC 27,853 was used as a quality control in the susceptibility test.

Techniques: Control, Inhibition

Journal: International Journal of Molecular Sciences

Article Title: Development and Validation of the MAST ISOPLEX ® VTEC Kit for Simultaneous Detection of Shiga Toxin/Verotoxin 1 and 2 ( stx1/vt1 and stx2/vt2 ) with Inhibition Control (IC) in a Rapid Loop-Mediated Isothermal Amplification (LAMP) Multiplex Assay

doi: 10.3390/ijms251810067

Figure Lengend Snippet: Assessment of the MAST ISOPLEX ® VTEC Kit Specificity.

Article Snippet: Pseudomonas aeruginosa , ATCC , 12,903 , ND , ND , 12.29 , Valid. Negative for verotoxigenic E. coli.

Techniques: Lamp Assay, Control

Journal: Heliyon

Article Title: Establishment of novel anti-TIM-3 antibodies interfering with its binding to ligands

doi: 10.1016/j.heliyon.2024.e28126

Figure Lengend Snippet: Alignment of the V H and V L amino acid sequences in TIM-3 antibodies. The framework regions (FRs) and complementarity-determining regions (CDRs) within the V H and V L regions were highlighted accordingly. The absence of no amino acid at a certain location is indicated by the symbol "-".

Article Snippet: Microplate wells were coated with recombinant proteins overnight at 4 °C, including human TIM-3-His (hTIM-3; Sino Biological, Beijing, China; 10390-H08H), CD137-His (hCD137; Sino Biological, 10041-H08H), OX40-His (hOX40; Sino Biological, 10481-H08H), PD-L1-His (hPD-L1; Sino Biological, 10084-H08H), mouse TIM-3-His (mTIM-3; Sino Biological, 51152-M08H), or cynomolgus TIM-3-hFc (cynTIM-3; Sino Biological, 90312-C02H) at a concentration of 1 μg/mL.

Techniques:

Journal: Heliyon

Article Title: Establishment of novel anti-TIM-3 antibodies interfering with its binding to ligands

doi: 10.1016/j.heliyon.2024.e28126

Figure Lengend Snippet: Analysis of the specificities of TIM-3 antibodies. (A) The purified TIM-3-specific mouse IgG antibodies (Abs) were subjected to SDS-PAGE analysis under non-denaturing (left panel) and denaturing (right panel) conditions. The separate images were cropped from the same gel. The entire image of the gel is shown in The recognition characteristics of TIM-3 Abs for native and denatured TIM-3 protein in western blot analysis. The combined images were cropped from different images. The entire image of western blots is shown in The specificity of the anti-TIM-3 mAbs was assess by ELISAs. The columns represent mean ± standard deviation (SD) of triplicates. Statistical significance was indicated as ****p < 0.0001, ***p < 0.001, and *p < 0.05 compared with Blank. Consistent results were observed in three independent experiments. (D, E) Reactivities of anti-TIM-3 mAbs towards endogenous cell surface TIM-3 protein on the RPMI8226 multiple myeloma cell line were assessed using flow cytometry. The RPMI8226 cells without addition of TIM-3 mAb were used as control (Con). Representative dot plots (D) and histograms (E) depicting the TIM-3 expression by flow cytometry were generated. M: molecular weight marker; 1 = MsT001; 2 = MsT065; 3 = MsT229; 4 = MsT286; 5 = non-denatured TIM-3 protein; 6 = denatured TIM-3 protein; hTIM-3 = human TIM-3; cynTIM-3 = cynomolgus TIM-3; mTIM-3 = mouse TIM-3; hCD137 = human CD137; hPD-L1 = human PD-L1; hOX40 = human OX40.

Article Snippet: Microplate wells were coated with recombinant proteins overnight at 4 °C, including human TIM-3-His (hTIM-3; Sino Biological, Beijing, China; 10390-H08H), CD137-His (hCD137; Sino Biological, 10041-H08H), OX40-His (hOX40; Sino Biological, 10481-H08H), PD-L1-His (hPD-L1; Sino Biological, 10084-H08H), mouse TIM-3-His (mTIM-3; Sino Biological, 51152-M08H), or cynomolgus TIM-3-hFc (cynTIM-3; Sino Biological, 90312-C02H) at a concentration of 1 μg/mL.

Techniques: Purification, SDS Page, Western Blot, Standard Deviation, Flow Cytometry, Expressing, Generated, Molecular Weight, Marker

Journal: Heliyon

Article Title: Establishment of novel anti-TIM-3 antibodies interfering with its binding to ligands

doi: 10.1016/j.heliyon.2024.e28126

Figure Lengend Snippet: Binding characteristics of TIM-3-specific antibodies. (A) Identification of distinct epitopes of TIM-3 through sandwich ELISAs. (B) The verification of various epitopes on TIM-3 using the ForteBio Octet system. A sensorgram shows TIM-3-Fc on the anti-human IgG Fc capture sensors, which has been linked to an anti-TIM-3 antibody to achieve saturation, and then bind to all TIM-3 antibodies. Statistical significance was indicated as ****p < 0.0001 and *p < 0.05 compared with Blank.

Article Snippet: Microplate wells were coated with recombinant proteins overnight at 4 °C, including human TIM-3-His (hTIM-3; Sino Biological, Beijing, China; 10390-H08H), CD137-His (hCD137; Sino Biological, 10041-H08H), OX40-His (hOX40; Sino Biological, 10481-H08H), PD-L1-His (hPD-L1; Sino Biological, 10084-H08H), mouse TIM-3-His (mTIM-3; Sino Biological, 51152-M08H), or cynomolgus TIM-3-hFc (cynTIM-3; Sino Biological, 90312-C02H) at a concentration of 1 μg/mL.

Techniques: Binding Assay

Journal: Heliyon

Article Title: Establishment of novel anti-TIM-3 antibodies interfering with its binding to ligands

doi: 10.1016/j.heliyon.2024.e28126

Figure Lengend Snippet: Sensitivities of TIM-3-specific antibodies. (A) Sensitivities of unlabeled (left) and biotin-labeled (right) TIM-3 monoclonal antibodies were determined by indirect ELISAs. (B) The sensitivities of anti-TIM-3 antibody pairs in sandwich ELISA assays.

Article Snippet: Microplate wells were coated with recombinant proteins overnight at 4 °C, including human TIM-3-His (hTIM-3; Sino Biological, Beijing, China; 10390-H08H), CD137-His (hCD137; Sino Biological, 10041-H08H), OX40-His (hOX40; Sino Biological, 10481-H08H), PD-L1-His (hPD-L1; Sino Biological, 10084-H08H), mouse TIM-3-His (mTIM-3; Sino Biological, 51152-M08H), or cynomolgus TIM-3-hFc (cynTIM-3; Sino Biological, 90312-C02H) at a concentration of 1 μg/mL.

Techniques: Labeling, Sandwich ELISA

Journal: Heliyon

Article Title: Establishment of novel anti-TIM-3 antibodies interfering with its binding to ligands

doi: 10.1016/j.heliyon.2024.e28126

Figure Lengend Snippet: TIM-3 antibodies' affinities for human TIM-3 as assessed by the ForteBio Octet system. The original sensorgram shows TIM-3-Fc on the anti-human IgG Fc capture sensors, which bound to various concentrations of TIM-3 antibodies. The binding affinity parameter K D was determined by fitting the sensorgram. R 2 is the coefficient of determination to estimate the goodness of the curve fit as reported by ForteBio Data Analysis Software 9.0.

Article Snippet: Microplate wells were coated with recombinant proteins overnight at 4 °C, including human TIM-3-His (hTIM-3; Sino Biological, Beijing, China; 10390-H08H), CD137-His (hCD137; Sino Biological, 10041-H08H), OX40-His (hOX40; Sino Biological, 10481-H08H), PD-L1-His (hPD-L1; Sino Biological, 10084-H08H), mouse TIM-3-His (mTIM-3; Sino Biological, 51152-M08H), or cynomolgus TIM-3-hFc (cynTIM-3; Sino Biological, 90312-C02H) at a concentration of 1 μg/mL.

Techniques: Binding Assay, Software

Journal: Heliyon

Article Title: Establishment of novel anti-TIM-3 antibodies interfering with its binding to ligands

doi: 10.1016/j.heliyon.2024.e28126

Figure Lengend Snippet: The sensitivities and affinities of TIM-3 antibodies to cynomolgus TIM-3 protein. (A) The sensitivities of the unlabeled and biotin-labeled TIM-3 antibodies were assessed by ELISAs. (B) The affinities of the TIM-3 antibodies for cynTIM-3 were assessed using the ForteBio Octet system.

Article Snippet: Microplate wells were coated with recombinant proteins overnight at 4 °C, including human TIM-3-His (hTIM-3; Sino Biological, Beijing, China; 10390-H08H), CD137-His (hCD137; Sino Biological, 10041-H08H), OX40-His (hOX40; Sino Biological, 10481-H08H), PD-L1-His (hPD-L1; Sino Biological, 10084-H08H), mouse TIM-3-His (mTIM-3; Sino Biological, 51152-M08H), or cynomolgus TIM-3-hFc (cynTIM-3; Sino Biological, 90312-C02H) at a concentration of 1 μg/mL.

Techniques: Labeling

Journal: Heliyon

Article Title: Establishment of novel anti-TIM-3 antibodies interfering with its binding to ligands

doi: 10.1016/j.heliyon.2024.e28126

Figure Lengend Snippet: The binding characteristics of anti-TIM-3 antibodies to ligand/TIM-3 complex. The binding of mouse anti-human TIM-3 antibodies to human Gal-9/TIM-3 (B), CEACAM-1/TIM-3 (C) or HMGB-1/TIM-3 complex (D) was detected using anti-mouse antibodies in ELISAs. Prior to being treated with mouse anti-TIM-3 mAbs, TIM-3-His were firstly attached to immobilized Gal-9, CEACAM-1, or HMGB-1 in ELISA plates. Reaction complexes were identified using anti-Ms-(H + L)-HRP. NC = negative control without TIM-3 protein; Blank = blank control without an anti-TIM-3 antibody. Horizontal lines indicate the cut-off, which were determined based on 2.1 times the mean absorbance of the negative control or blank. Statistical significance was indicated as ****p < 0.0001, ***p < 0.001, **p < 0.01, and *p < 0.05 compared with NC or Blank.

Article Snippet: Microplate wells were coated with recombinant proteins overnight at 4 °C, including human TIM-3-His (hTIM-3; Sino Biological, Beijing, China; 10390-H08H), CD137-His (hCD137; Sino Biological, 10041-H08H), OX40-His (hOX40; Sino Biological, 10481-H08H), PD-L1-His (hPD-L1; Sino Biological, 10084-H08H), mouse TIM-3-His (mTIM-3; Sino Biological, 51152-M08H), or cynomolgus TIM-3-hFc (cynTIM-3; Sino Biological, 90312-C02H) at a concentration of 1 μg/mL.

Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Negative Control

Journal: Heliyon

Article Title: Establishment of novel anti-TIM-3 antibodies interfering with its binding to ligands

doi: 10.1016/j.heliyon.2024.e28126

Figure Lengend Snippet: Anti-TIM-3 antibodies inhibited Gal-9 from binding to TIM-3. Binding of hTIM-3-His to plate-bound Gal-9 was evaluated via ELISA in the presence of anti-TIM-3 antibodies. The reaction complex was detected using anti-His-HRP or anti-Ms-(H + L)-HRP. Horizontal lines indicate the cut-off, which were determined based on 2.1 times the mean absorbance of the negative control without TIM-3 protein. Statistical significance was assessed using multiple unpaired t-tests (**p < 0.01 compared with negative control). NS indicates no statistically significance.

Article Snippet: Microplate wells were coated with recombinant proteins overnight at 4 °C, including human TIM-3-His (hTIM-3; Sino Biological, Beijing, China; 10390-H08H), CD137-His (hCD137; Sino Biological, 10041-H08H), OX40-His (hOX40; Sino Biological, 10481-H08H), PD-L1-His (hPD-L1; Sino Biological, 10084-H08H), mouse TIM-3-His (mTIM-3; Sino Biological, 51152-M08H), or cynomolgus TIM-3-hFc (cynTIM-3; Sino Biological, 90312-C02H) at a concentration of 1 μg/mL.

Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Negative Control