9000 mwco protein concentrator  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 9000 mwco protein concentrator
    9000 Mwco Protein Concentrator, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/9000 mwco protein concentrator/product/Thermo Fisher
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    9000 mwco protein concentrator - by Bioz Stars, 2020-04
    87/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Dissecting Protein Function: An Efficient Protocol for Identifying Separation-of-Function Mutations That Encode Structurally Stable Proteins
    Article Snippet: Soluble Est3 mutant proteins were purified from the clarified cell lysate, which was subjected to Ni2+ -affinity chromatography by gravity flow (GE Healthcare). .. Est3 eluted at > 95% purity, as assessed on Coomassie-stained gels , and was concentrated and buffer-exchanged to 50 mM potassium phosphate buffer (pH 7.4) using a 9000 MWCO protein concentrator (Pierce).

    Affinity Chromatography:

    Article Title: Dissecting Protein Function: An Efficient Protocol for Identifying Separation-of-Function Mutations That Encode Structurally Stable Proteins
    Article Snippet: The contaminating ∼14-kDa His10 -SUMO tag was separated from the ∼20.6-kDa Est3 protein by a second round of Ni2+ -affinity chromatography in buffer B containing 20 mM imidazole. .. Est3 eluted at > 95% purity, as assessed on Coomassie-stained gels , and was concentrated and buffer-exchanged to 50 mM potassium phosphate buffer (pH 7.4) using a 9000 MWCO protein concentrator (Pierce).

    Mutagenesis:

    Article Title: Dissecting Protein Function: An Efficient Protocol for Identifying Separation-of-Function Mutations That Encode Structurally Stable Proteins
    Article Snippet: Paragraph title: Expression and purification of wild-type and mutant Est3 proteins ... Est3 eluted at > 95% purity, as assessed on Coomassie-stained gels , and was concentrated and buffer-exchanged to 50 mM potassium phosphate buffer (pH 7.4) using a 9000 MWCO protein concentrator (Pierce).

    Buffer Exchange:

    Article Title: Dissecting Protein Function: An Efficient Protocol for Identifying Separation-of-Function Mutations That Encode Structurally Stable Proteins
    Article Snippet: The His10 -SUMO-Est3 proteins were subsequently eluted with buffer A containing 300 mM imidazole and concentrated in a 10,000 MWCO concentrator (Millipore) to 2 ml, without buffer exchange, and incubated overnight at 4° with the Ulp1 SUMO isopeptidase to cleave the His10 -SUMO tag ( ). .. Est3 eluted at > 95% purity, as assessed on Coomassie-stained gels , and was concentrated and buffer-exchanged to 50 mM potassium phosphate buffer (pH 7.4) using a 9000 MWCO protein concentrator (Pierce).

    Size-exclusion Chromatography:

    Article Title: Dissecting Protein Function: An Efficient Protocol for Identifying Separation-of-Function Mutations That Encode Structurally Stable Proteins
    Article Snippet: Cleaved proteins were purified via size-exclusion chromatography (Superdex75; GE Healthcare) on an AKTA FPLC system in buffer B (100 mM KH2 PO4 /K3 PO4 , pH 7.5, 100 mM Na2 SO4 , 5% glycerol, and 3 mM β-ME). .. Est3 eluted at > 95% purity, as assessed on Coomassie-stained gels , and was concentrated and buffer-exchanged to 50 mM potassium phosphate buffer (pH 7.4) using a 9000 MWCO protein concentrator (Pierce).

    Solubility:

    Article Title: Dissecting Protein Function: An Efficient Protocol for Identifying Separation-of-Function Mutations That Encode Structurally Stable Proteins
    Article Snippet: Aliquots of the pellet and supernatant fractions were resolved on a 13% SDS-PAGE gel, and solubility was assessed by Western blot analysis against the His tag to compare the His10 -SUMO-Est3 bands from the two fractions ( ). .. Est3 eluted at > 95% purity, as assessed on Coomassie-stained gels , and was concentrated and buffer-exchanged to 50 mM potassium phosphate buffer (pH 7.4) using a 9000 MWCO protein concentrator (Pierce).

    Purification:

    Article Title: Dissecting Protein Function: An Efficient Protocol for Identifying Separation-of-Function Mutations That Encode Structurally Stable Proteins
    Article Snippet: Paragraph title: Expression and purification of wild-type and mutant Est3 proteins ... Est3 eluted at > 95% purity, as assessed on Coomassie-stained gels , and was concentrated and buffer-exchanged to 50 mM potassium phosphate buffer (pH 7.4) using a 9000 MWCO protein concentrator (Pierce).

    Incubation:

    Article Title: Dissecting Protein Function: An Efficient Protocol for Identifying Separation-of-Function Mutations That Encode Structurally Stable Proteins
    Article Snippet: The His10 -SUMO-Est3 proteins were subsequently eluted with buffer A containing 300 mM imidazole and concentrated in a 10,000 MWCO concentrator (Millipore) to 2 ml, without buffer exchange, and incubated overnight at 4° with the Ulp1 SUMO isopeptidase to cleave the His10 -SUMO tag ( ). .. Est3 eluted at > 95% purity, as assessed on Coomassie-stained gels , and was concentrated and buffer-exchanged to 50 mM potassium phosphate buffer (pH 7.4) using a 9000 MWCO protein concentrator (Pierce).

    Fast Protein Liquid Chromatography:

    Article Title: Dissecting Protein Function: An Efficient Protocol for Identifying Separation-of-Function Mutations That Encode Structurally Stable Proteins
    Article Snippet: Cleaved proteins were purified via size-exclusion chromatography (Superdex75; GE Healthcare) on an AKTA FPLC system in buffer B (100 mM KH2 PO4 /K3 PO4 , pH 7.5, 100 mM Na2 SO4 , 5% glycerol, and 3 mM β-ME). .. Est3 eluted at > 95% purity, as assessed on Coomassie-stained gels , and was concentrated and buffer-exchanged to 50 mM potassium phosphate buffer (pH 7.4) using a 9000 MWCO protein concentrator (Pierce).

    Expressing:

    Article Title: Dissecting Protein Function: An Efficient Protocol for Identifying Separation-of-Function Mutations That Encode Structurally Stable Proteins
    Article Snippet: Paragraph title: Expression and purification of wild-type and mutant Est3 proteins ... Est3 eluted at > 95% purity, as assessed on Coomassie-stained gels , and was concentrated and buffer-exchanged to 50 mM potassium phosphate buffer (pH 7.4) using a 9000 MWCO protein concentrator (Pierce).

    Western Blot:

    Article Title: Dissecting Protein Function: An Efficient Protocol for Identifying Separation-of-Function Mutations That Encode Structurally Stable Proteins
    Article Snippet: Aliquots of the pellet and supernatant fractions were resolved on a 13% SDS-PAGE gel, and solubility was assessed by Western blot analysis against the His tag to compare the His10 -SUMO-Est3 bands from the two fractions ( ). .. Est3 eluted at > 95% purity, as assessed on Coomassie-stained gels , and was concentrated and buffer-exchanged to 50 mM potassium phosphate buffer (pH 7.4) using a 9000 MWCO protein concentrator (Pierce).

    SDS Page:

    Article Title: Dissecting Protein Function: An Efficient Protocol for Identifying Separation-of-Function Mutations That Encode Structurally Stable Proteins
    Article Snippet: Aliquots of the pellet and supernatant fractions were resolved on a 13% SDS-PAGE gel, and solubility was assessed by Western blot analysis against the His tag to compare the His10 -SUMO-Est3 bands from the two fractions ( ). .. Est3 eluted at > 95% purity, as assessed on Coomassie-stained gels , and was concentrated and buffer-exchanged to 50 mM potassium phosphate buffer (pH 7.4) using a 9000 MWCO protein concentrator (Pierce).