rabbit monoclonal antibodies against cd68  (Sino Biological)


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    Structured Review

    Sino Biological rabbit monoclonal antibodies against cd68
    14-3-3η is expressed in <t>CD68</t> + cells but not in CD4 + T cells from the synovium of RA patients. Synovium specimens of patients with RA ( n = 3) were analysed by IF using specific antibodies against 14-3-3η and CD68 ( a ) or CD4 ( b ). c Lung tissue specimens of patients with RA ( n = 3) were analysed using specific antibodies against 14-3-3η and CD68. Representative images of three independent experiments are shown. Scale bar, 50 μm
    Rabbit Monoclonal Antibodies Against Cd68, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal antibodies against cd68/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal antibodies against cd68 - by Bioz Stars, 2021-02
    93/100 stars

    Images

    1) Product Images from "Tumour necrosis factor alpha promotes secretion of 14-3-3η by inducing necroptosis in macrophages"

    Article Title: Tumour necrosis factor alpha promotes secretion of 14-3-3η by inducing necroptosis in macrophages

    Journal: Arthritis Research & Therapy

    doi: 10.1186/s13075-020-2110-9

    14-3-3η is expressed in CD68 + cells but not in CD4 + T cells from the synovium of RA patients. Synovium specimens of patients with RA ( n = 3) were analysed by IF using specific antibodies against 14-3-3η and CD68 ( a ) or CD4 ( b ). c Lung tissue specimens of patients with RA ( n = 3) were analysed using specific antibodies against 14-3-3η and CD68. Representative images of three independent experiments are shown. Scale bar, 50 μm
    Figure Legend Snippet: 14-3-3η is expressed in CD68 + cells but not in CD4 + T cells from the synovium of RA patients. Synovium specimens of patients with RA ( n = 3) were analysed by IF using specific antibodies against 14-3-3η and CD68 ( a ) or CD4 ( b ). c Lung tissue specimens of patients with RA ( n = 3) were analysed using specific antibodies against 14-3-3η and CD68. Representative images of three independent experiments are shown. Scale bar, 50 μm

    Techniques Used:

    PAD4 is localized in CD68 + cells and CD55 + cells in the RA synovium. Synovium ( n = 3) specimens of patients with RA were assessed by IF using primary antibodies against PAD4 and 14-3-3η ( a ), PAD4 and CD68 ( b ), 14-3-3η and CD55 ( c ), or PAD4 and CD55 ( d ) and by FITC or rhodamine-labelled secondary antibodies. Representative images from three independent experiments are shown. Scale bar, 50 μm
    Figure Legend Snippet: PAD4 is localized in CD68 + cells and CD55 + cells in the RA synovium. Synovium ( n = 3) specimens of patients with RA were assessed by IF using primary antibodies against PAD4 and 14-3-3η ( a ), PAD4 and CD68 ( b ), 14-3-3η and CD55 ( c ), or PAD4 and CD55 ( d ) and by FITC or rhodamine-labelled secondary antibodies. Representative images from three independent experiments are shown. Scale bar, 50 μm

    Techniques Used:

    Related Articles

    Immunohistochemistry:

    Article Title: Tumour necrosis factor alpha promotes secretion of 14-3-3η by inducing necroptosis in macrophages
    Article Snippet: .. Antibodies and reagentsThe following antibodies were purchased and used for immunohistochemistry (IHC), immunofluorescence (IF), immunocytochemistry (ICC), and immunoblotting (IB) experiments: mouse monoclonal antibodies against 14-3-3η (1:200; AAM-11-0003, Augurex, Vancouver, BC, Canada) and 14-3-3γ (1:200; sc-731, Santa Cruz Biotechnology, Dallas, TX) for IHC, CD4 (1:200; ab67001, Abcam, Cambridge, MA) for IF, CD55 (1:200; ab1422, Abcam) for IF, 14-3-3η (1:200; NBP1-92691SS, Novus Biologicals, Centennial, CO) and phospho-Akt (1:200; Ser473) (#9271 Cell Signaling Technology, Danvers, MA) for ICC, and β-actin (1:10,000; A1978, Sigma-Aldrich, St. Louis, MO) for IB; rabbit monoclonal antibodies against CD68 (1:200; 11192-RP02, Sino Biological Inc., Wayne, PA), PAD4 (1:200; 17373-1, ProteinTech, Rosemont, IL), and phospho-RIP3 (Ser227) (1:200; 93654S, CST) for IF and ICC, and MLKL (1:2000; phospho S358, ab187091, Abcam) for IB; rabbit polyclonal antibodies against MLKL (1:2000; ab194699, Abcam) for IB. .. Rhodamine-labelled anti-mouse IgG antibody (F31663, Thermo Scientific, Suwanee, GA), FITC anti-rabbit IgG antibody (F9887, Sigma-Aldrich, St. Louis, MO), 4′,6-diamidino-2-phenylindole (DAPI; sc-3598, SCB), and rhodamine phalloidin (R415, Thermo Fisher Scientific, Waltham, MA) were used for IF.

    Immunocytochemistry:

    Article Title: Tumour necrosis factor alpha promotes secretion of 14-3-3η by inducing necroptosis in macrophages
    Article Snippet: .. Antibodies and reagentsThe following antibodies were purchased and used for immunohistochemistry (IHC), immunofluorescence (IF), immunocytochemistry (ICC), and immunoblotting (IB) experiments: mouse monoclonal antibodies against 14-3-3η (1:200; AAM-11-0003, Augurex, Vancouver, BC, Canada) and 14-3-3γ (1:200; sc-731, Santa Cruz Biotechnology, Dallas, TX) for IHC, CD4 (1:200; ab67001, Abcam, Cambridge, MA) for IF, CD55 (1:200; ab1422, Abcam) for IF, 14-3-3η (1:200; NBP1-92691SS, Novus Biologicals, Centennial, CO) and phospho-Akt (1:200; Ser473) (#9271 Cell Signaling Technology, Danvers, MA) for ICC, and β-actin (1:10,000; A1978, Sigma-Aldrich, St. Louis, MO) for IB; rabbit monoclonal antibodies against CD68 (1:200; 11192-RP02, Sino Biological Inc., Wayne, PA), PAD4 (1:200; 17373-1, ProteinTech, Rosemont, IL), and phospho-RIP3 (Ser227) (1:200; 93654S, CST) for IF and ICC, and MLKL (1:2000; phospho S358, ab187091, Abcam) for IB; rabbit polyclonal antibodies against MLKL (1:2000; ab194699, Abcam) for IB. .. Rhodamine-labelled anti-mouse IgG antibody (F31663, Thermo Scientific, Suwanee, GA), FITC anti-rabbit IgG antibody (F9887, Sigma-Aldrich, St. Louis, MO), 4′,6-diamidino-2-phenylindole (DAPI; sc-3598, SCB), and rhodamine phalloidin (R415, Thermo Fisher Scientific, Waltham, MA) were used for IF.

    Immunofluorescence:

    Article Title: Tumour necrosis factor alpha promotes secretion of 14-3-3η by inducing necroptosis in macrophages
    Article Snippet: .. Antibodies and reagentsThe following antibodies were purchased and used for immunohistochemistry (IHC), immunofluorescence (IF), immunocytochemistry (ICC), and immunoblotting (IB) experiments: mouse monoclonal antibodies against 14-3-3η (1:200; AAM-11-0003, Augurex, Vancouver, BC, Canada) and 14-3-3γ (1:200; sc-731, Santa Cruz Biotechnology, Dallas, TX) for IHC, CD4 (1:200; ab67001, Abcam, Cambridge, MA) for IF, CD55 (1:200; ab1422, Abcam) for IF, 14-3-3η (1:200; NBP1-92691SS, Novus Biologicals, Centennial, CO) and phospho-Akt (1:200; Ser473) (#9271 Cell Signaling Technology, Danvers, MA) for ICC, and β-actin (1:10,000; A1978, Sigma-Aldrich, St. Louis, MO) for IB; rabbit monoclonal antibodies against CD68 (1:200; 11192-RP02, Sino Biological Inc., Wayne, PA), PAD4 (1:200; 17373-1, ProteinTech, Rosemont, IL), and phospho-RIP3 (Ser227) (1:200; 93654S, CST) for IF and ICC, and MLKL (1:2000; phospho S358, ab187091, Abcam) for IB; rabbit polyclonal antibodies against MLKL (1:2000; ab194699, Abcam) for IB. .. Rhodamine-labelled anti-mouse IgG antibody (F31663, Thermo Scientific, Suwanee, GA), FITC anti-rabbit IgG antibody (F9887, Sigma-Aldrich, St. Louis, MO), 4′,6-diamidino-2-phenylindole (DAPI; sc-3598, SCB), and rhodamine phalloidin (R415, Thermo Fisher Scientific, Waltham, MA) were used for IF.