allprep dna rna ffpe kit  (Qiagen)


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    Name:
    AllPrep DNA RNA FFPE Kit
    Description:
    For simultaneous purification of genomic DNA and total RNA including small RNAs from formalin fixed paraffin embedded tissue sections Kit contents Qiagen AllPrep DNA RNA FFPE Kit 50 preps FFPE Tissue Sample RNA 14 to 30L DNA 30 to 100L Elution Volume Silica Technology Manual Processing For Simultaneous Purification of Genomic DNA and Total RNA from Formalin fixed Paraffin embedded Tissue Sections Ideal for PCR qPCR Real time RT PCR Microarray Includes 50 RNeasy minElute Spin Columns 50 QIAamp minElute Spin Columns Collection Tubes RNase free Reagents and Buffers Benefits Maximum output with minimal sample consumption Releases DNA RNA without compromising integrity Effectively separates RNA and DNA Comprehensive DNA and RNA analysis of the same FFPE sam
    Catalog Number:
    80234
    Price:
    660
    Category:
    AllPrep DNA RNA FFPE Kit
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    Structured Review

    Qiagen allprep dna rna ffpe kit
    AllPrep DNA RNA FFPE Kit
    For simultaneous purification of genomic DNA and total RNA including small RNAs from formalin fixed paraffin embedded tissue sections Kit contents Qiagen AllPrep DNA RNA FFPE Kit 50 preps FFPE Tissue Sample RNA 14 to 30L DNA 30 to 100L Elution Volume Silica Technology Manual Processing For Simultaneous Purification of Genomic DNA and Total RNA from Formalin fixed Paraffin embedded Tissue Sections Ideal for PCR qPCR Real time RT PCR Microarray Includes 50 RNeasy minElute Spin Columns 50 QIAamp minElute Spin Columns Collection Tubes RNase free Reagents and Buffers Benefits Maximum output with minimal sample consumption Releases DNA RNA without compromising integrity Effectively separates RNA and DNA Comprehensive DNA and RNA analysis of the same FFPE sam
    https://www.bioz.com/result/allprep dna rna ffpe kit/product/Qiagen
    Average 90 stars, based on 66 article reviews
    Price from $9.99 to $1999.99
    allprep dna rna ffpe kit - by Bioz Stars, 2020-01
    90/100 stars

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    Related Articles

    DNA Extraction:

    Article Title: Increased FGF19 copy number is frequently detected in hepatocellular carcinoma with a complete response after sorafenib treatment
    Article Snippet: Paragraph title: DNA extraction ... The DNA was purified using an Allprep DNA/RNA FFPE kit (Qiagen, Valencia, CA) according to the manufacturer's instructions.

    Article Title: A new species of Xenoturbella from the western Pacific Ocean and the evolution of Xenoturbella
    Article Snippet: Paragraph title: DNA extraction and sequencing ... Genomic DNA of the X. japonica paratype specimen was extracted using Allprep DNA/RNA FFPE Kit (QIAGEN).

    Article Title: Analytical Validation of the Next-Generation Sequencing Assay for a Nationwide Signal-Finding Clinical Trial
    Article Snippet: These archived samples had undergone different nucleic extraction methods: FNLCR used Qiagen AllPrep DNA/RNA FFPE kit (Qiagen, Valencia, CA); MDACC extracted DNA using the PicoPure DNA Extraction Kit (Arcturus, Mountain View, CA) and extracted RNA using the Agencourt AMPureXP Kit (Agencourt Biosciences, Beverly, MA); MGH extracted total nucleic acids using the Agencourt FormaPure kit; and YSM extracted DNA using the QIAamp DNA FFPE Tissue Kit and RNA using the Qiagen RNeasy FFPE kit. .. Nucleic acid samples used in other assessments and the fit-for-purpose study (and subsequently for the NCI-MATCH trial tumor specimen) were extracted by Qiagen AllPrep DNA/RNA FFPE kit.

    Article Title: 46,XY Disorders of Sex Development and Congenital Diaphragmatic Hernia: A Case with Dysmorphic Facies, Truncus Arteriosus, Bifid Thymus, Gut Malrotation, Rhizomelia, and Adactyly
    Article Snippet: Paragraph title: DNA Extraction and Quantification ... Following the manufacturer’s protocol, genomic DNA was extracted from liver, spleen, adrenal, and kidney tissues from the patient using AllPrep DNA/RNA FFPE kit (Qiagen, Valencia, CA).

    Centrifugation:

    Article Title: Molecular Diagnosis Using Residual Liquid-Based Cytology Materials for Patients with Nondiagnostic or Indeterminate Thyroid Nodules
    Article Snippet: DNA and RNA extraction from residual SurePath material Centrifugation of residual SurePath material was performed at 20,000 rpm for 2 minutes and the pellet was used. .. DNAs and RNAs were extracted from the samples using the AllPrep DNA/RNA FFPE Kit (Qiagen, Hilden, Germany).

    Amplification:

    Article Title: A new species of Xenoturbella from the western Pacific Ocean and the evolution of Xenoturbella
    Article Snippet: PCR conditions (taq-polymerases, primers and amplification parameters) are shown in Additional file : Table S4. .. Genomic DNA of the X. japonica paratype specimen was extracted using Allprep DNA/RNA FFPE Kit (QIAGEN).

    Article Title: PARP1 Is Up-Regulated in Non-small Cell Lung Cancer Tissues in the Presence of the Cyanobacterial Toxin Microcystin
    Article Snippet: For the FFPE samples, total DNA was isolated from samples using AllPrep DNA/RNA FFPE kit (Qiagen) and QIAamp DNA microbiome kit (Qiagen) following manufacturer's protocol with modifications. .. The availability of microbial DNA was verified by PCR amplification using 16S rRNA specific primers, 27F (5′-AGAGTTTGATCMTGGCTCAG-3′) and 519R (5′-GWA TTA CCG CGG CKG CTG-3′).

    Expressing:

    Article Title: New Molecular Assay for the Proliferation Signature in Mantle Cell Lymphoma Applicable to Formalin-Fixed Paraffin-Embedded Biopsies
    Article Snippet: Paragraph title: Gene Expression Profiling ... Nucleic acids were extracted from 10-μm sections of FFPE biopsies using the QIAGEN AllPrep DNA/RNA FFPE Kit (QIAGEN, Hilden, Germany) after deparaffinization according to the manufacturer’s instructions.

    Article Title: TIAM1 promotes chemoresistance and tumor invasiveness in colorectal cancer
    Article Snippet: RNA isolation and quantitative reverse-transcription PCR RNA extraction from tissue samples was performed using the miRNeasy RNA isolation kit (Qiagen, Valencia, California, USA) and AllPrep DNA/RNA FFPE kit (Qiagen) following the manufacturer’s protocol. .. The average expression levels of the target genes were normalized against β-actin using the comparative CT method.

    Synthesized:

    Article Title: Three Dimensional Culture of Human Renal Cell Carcinoma Organoids
    Article Snippet: Construct Molecular Analysis DNA and RNA were isolated from FFPE sections using the AllPrep DNA/RNA FFPE kit (Qiagen). .. Isolated RNA was treated with DNase I (Qiagen) to eliminate potential DNA contamination prior to downstream analysis. cDNA was synthesized using random primers (Ambion, Life Technologies) and the Sensiscript Reverse Transcriptase kit (Qiagen).

    Picogreen Assay:

    Article Title: Increased FGF19 copy number is frequently detected in hepatocellular carcinoma with a complete response after sorafenib treatment
    Article Snippet: The DNA was purified using an Allprep DNA/RNA FFPE kit (Qiagen, Valencia, CA) according to the manufacturer's instructions. .. The quality and quantity of the DNA were verified using the NanoDrop 2000 device (Thermo Scientific, Wilmington, DE) and the PicoGreen dsDNA assay kit (Life Technologies, Foster City, CA).

    Blocking Assay:

    Article Title: Evaluation of commercial DNA and RNA extraction methods for high-throughput sequencing of FFPE samples
    Article Snippet: The QIAamp DNA FFPE Tissue Kit, miRNeasy FFPE Kit and AllPrep DNA/RNA FFPE Kit from QIAGEN have been shown to perform well in comparison with other DNA and RNA protocols in previous studies [ , , ]. .. Equal amount of starting material and a randomized order of the consecutive FFPE block sections were used for all methods of extraction.

    Article Title: Random forest-based modelling to detect biomarkers for prostate cancer progression
    Article Snippet: .. Three tissue punches (0.6 mm × 3 mm) were taken of each tissue block, and genomic DNA was isolated using the AllPrep® DNA/RNA FFPE kit (Qiagen). .. DNA was submitted to the DKFZ Genome and Proteome core facility for Illumina 450k Methylation analyses.

    Article Title: PARP1 Is Up-Regulated in Non-small Cell Lung Cancer Tissues in the Presence of the Cyanobacterial Toxin Microcystin
    Article Snippet: In addition, we derived our negative controls from biopsied paraffin block adjacent to the lung tissues and from sterilized and microbial nuclease-free filtered water used in our reagents. .. For the FFPE samples, total DNA was isolated from samples using AllPrep DNA/RNA FFPE kit (Qiagen) and QIAamp DNA microbiome kit (Qiagen) following manufacturer's protocol with modifications.

    Real-time Polymerase Chain Reaction:

    Article Title: TIAM1 promotes chemoresistance and tumor invasiveness in colorectal cancer
    Article Snippet: RNA isolation and quantitative reverse-transcription PCR RNA extraction from tissue samples was performed using the miRNeasy RNA isolation kit (Qiagen, Valencia, California, USA) and AllPrep DNA/RNA FFPE kit (Qiagen) following the manufacturer’s protocol. .. We performed quantitative real-time reverse-transcription analysis (qRT-PCR) using the SensiFAST™ SYBR® Lo-ROX Kit (Bioline, London, UK) and the Quantstudio 7 Real-Time PCR System (Life Technologies, Carlsbad, CA, USA).

    Formalin-fixed Paraffin-Embedded:

    Article Title: Concurrent leukoencephalomyelitis and polyneuritis in a Maltese terrier: resembling combined central and peripheral demyelination in humans
    Article Snippet: .. DNA and RNA were extracted from the formalin-fixed, paraffin-embedded cerebrum, cerebellum, and sciatic nerves using an AllPrep DNA/RNA FFPE kit, (Qiagen, Valencia, CA, U.S.A.). .. RNA reverse transcription was performed immediately with the QuantiTect® Reverse Transcription kit (Qiagen).

    Article Title: Three Dimensional Culture of Human Renal Cell Carcinoma Organoids
    Article Snippet: .. Construct Molecular Analysis DNA and RNA were isolated from FFPE sections using the AllPrep DNA/RNA FFPE kit (Qiagen). .. Isolated RNA was treated with DNase I (Qiagen) to eliminate potential DNA contamination prior to downstream analysis. cDNA was synthesized using random primers (Ambion, Life Technologies) and the Sensiscript Reverse Transcriptase kit (Qiagen).

    Article Title: Increased FGF19 copy number is frequently detected in hepatocellular carcinoma with a complete response after sorafenib treatment
    Article Snippet: .. The DNA was purified using an Allprep DNA/RNA FFPE kit (Qiagen, Valencia, CA) according to the manufacturer's instructions. .. The quality and quantity of the DNA were verified using the NanoDrop 2000 device (Thermo Scientific, Wilmington, DE) and the PicoGreen dsDNA assay kit (Life Technologies, Foster City, CA).

    Article Title: Evaluation of commercial DNA and RNA extraction methods for high-throughput sequencing of FFPE samples
    Article Snippet: .. The QIAamp DNA FFPE Tissue Kit, miRNeasy FFPE Kit and AllPrep DNA/RNA FFPE Kit from QIAGEN have been shown to perform well in comparison with other DNA and RNA protocols in previous studies [ , , ]. .. Equal amount of starting material and a randomized order of the consecutive FFPE block sections were used for all methods of extraction.

    Article Title: A new species of Xenoturbella from the western Pacific Ocean and the evolution of Xenoturbella
    Article Snippet: .. Genomic DNA of the X. japonica paratype specimen was extracted using Allprep DNA/RNA FFPE Kit (QIAGEN). .. The extracted genomic DNA was fragmented to a target length of 600 bp by S220 Focused-Ultrasonicator (Covaris).

    Article Title: New Molecular Assay for the Proliferation Signature in Mantle Cell Lymphoma Applicable to Formalin-Fixed Paraffin-Embedded Biopsies
    Article Snippet: .. Nucleic acids were extracted from 10-μm sections of FFPE biopsies using the QIAGEN AllPrep DNA/RNA FFPE Kit (QIAGEN, Hilden, Germany) after deparaffinization according to the manufacturer’s instructions. .. Gene expression was quantitated in 200 ng of RNA on the NanoString platform (NanoString Technologies, Seattle, WA), using the “high sensitivity” setting on the nCounter Prep Station and 490 fields of view on the nCounter analyzer (generation 2) or 1,155 fields of view when a generation 1 analyzer was used.

    Article Title: Random forest-based modelling to detect biomarkers for prostate cancer progression
    Article Snippet: .. Three tissue punches (0.6 mm × 3 mm) were taken of each tissue block, and genomic DNA was isolated using the AllPrep® DNA/RNA FFPE kit (Qiagen). .. DNA was submitted to the DKFZ Genome and Proteome core facility for Illumina 450k Methylation analyses.

    Article Title: Molecular Diagnosis Using Residual Liquid-Based Cytology Materials for Patients with Nondiagnostic or Indeterminate Thyroid Nodules
    Article Snippet: .. DNAs and RNAs were extracted from the samples using the AllPrep DNA/RNA FFPE Kit (Qiagen, Hilden, Germany). ..

    Article Title: Enzyme-free digital counting of endogenous circular RNA molecules in B-cell malignancies
    Article Snippet: .. For RNA isolation of FFPE cell lines, the Allprep DNA/RNA FFPE kit (Qiagen) was used following a xylene deparaffinization. .. RNA from fresh frozen patient samples was isolated with the RNeasy mini kit (Qiagen).

    Article Title: PARP1 Is Up-Regulated in Non-small Cell Lung Cancer Tissues in the Presence of the Cyanobacterial Toxin Microcystin
    Article Snippet: .. For the FFPE samples, total DNA was isolated from samples using AllPrep DNA/RNA FFPE kit (Qiagen) and QIAamp DNA microbiome kit (Qiagen) following manufacturer's protocol with modifications. .. For the fresh frozen samples, DNA, RNA and protein were extracted using Qiagen's AllPrep DNA/RNA/Protein Mini Kit (Orloff et al., ) (Qiagen, Valencia, CA, USA) according to manufacturer's protocol.

    Article Title: Ectopic expression of PLC‐β2 in non‐invasive breast tumor cells plays a protective role against malignant progression and is correlated with the deregulation of miR‐146a, et al. Ectopic expression of PLC‐β2 in non‐invasive breast tumor cells plays a protective role against malignant progression and is correlated with the deregulation of miR‐146a
    Article Snippet: .. Dissected areas were processed for RNA extraction using AllPrep DNA/RNA FFPE Kit (QIAGEN), following the manufacturer's instructions. .. The short RNA was profiled using an RNAseq approach on SOLiD (Thermo Fisher Scientific, Paisley, UK), following a previously described procedure.

    Article Title: Association between the expression of microRNAs and the response of patients with locally advanced rectal cancer to preoperative chemoradiotherapy
    Article Snippet: .. RNA extraction Total RNA was isolated from the FFPE tissue using the miRNeasy FFPE kit for the test study and the AllPrep DNA/RNA FFPE kit (both Qiagen GmbH, Hilden, Germany) for the validation study according to the manufacturer's protocol. ..

    Article Title: Analytical Validation of the Next-Generation Sequencing Assay for a Nationwide Signal-Finding Clinical Trial
    Article Snippet: .. Nucleic acid samples used in other assessments and the fit-for-purpose study (and subsequently for the NCI-MATCH trial tumor specimen) were extracted by Qiagen AllPrep DNA/RNA FFPE kit. .. All nucleic acid samples were quantitated by Qubit fluorometer (Thermo Fisher Scientific) before use in the NGS assay.

    Article Title: TIAM1 promotes chemoresistance and tumor invasiveness in colorectal cancer
    Article Snippet: .. RNA isolation and quantitative reverse-transcription PCR RNA extraction from tissue samples was performed using the miRNeasy RNA isolation kit (Qiagen, Valencia, California, USA) and AllPrep DNA/RNA FFPE kit (Qiagen) following the manufacturer’s protocol. .. The synthesis of complementary DNA (cDNA) was performed from 500 ng of total RNA using the High Capacity cDNA Reverse-Transcription Kit (Invitrogen, Carlsbad, CA, USA).

    Article Title: 46,XY Disorders of Sex Development and Congenital Diaphragmatic Hernia: A Case with Dysmorphic Facies, Truncus Arteriosus, Bifid Thymus, Gut Malrotation, Rhizomelia, and Adactyly
    Article Snippet: .. Following the manufacturer’s protocol, genomic DNA was extracted from liver, spleen, adrenal, and kidney tissues from the patient using AllPrep DNA/RNA FFPE kit (Qiagen, Valencia, CA). .. The DNA concentrations were quantified using the Qubit fluorometer (Life Technologies, Carlsbad, CA).

    Quantitative RT-PCR:

    Article Title: TIAM1 promotes chemoresistance and tumor invasiveness in colorectal cancer
    Article Snippet: RNA isolation and quantitative reverse-transcription PCR RNA extraction from tissue samples was performed using the miRNeasy RNA isolation kit (Qiagen, Valencia, California, USA) and AllPrep DNA/RNA FFPE kit (Qiagen) following the manufacturer’s protocol. .. We performed quantitative real-time reverse-transcription analysis (qRT-PCR) using the SensiFAST™ SYBR® Lo-ROX Kit (Bioline, London, UK) and the Quantstudio 7 Real-Time PCR System (Life Technologies, Carlsbad, CA, USA).

    Derivative Assay:

    Article Title: PARP1 Is Up-Regulated in Non-small Cell Lung Cancer Tissues in the Presence of the Cyanobacterial Toxin Microcystin
    Article Snippet: In addition, we derived our negative controls from biopsied paraffin block adjacent to the lung tissues and from sterilized and microbial nuclease-free filtered water used in our reagents. .. For the FFPE samples, total DNA was isolated from samples using AllPrep DNA/RNA FFPE kit (Qiagen) and QIAamp DNA microbiome kit (Qiagen) following manufacturer's protocol with modifications.

    Immunohistochemistry:

    Article Title: Concurrent leukoencephalomyelitis and polyneuritis in a Maltese terrier: resembling combined central and peripheral demyelination in humans
    Article Snippet: After exposure to 2% diaminobenzidine for 3 min, the slides were counterstained with hematoxylin for 30 sec. IHC stainings for CD11d (diluted 1:50; clone CA18.3C6; P.F. .. DNA and RNA were extracted from the formalin-fixed, paraffin-embedded cerebrum, cerebellum, and sciatic nerves using an AllPrep DNA/RNA FFPE kit, (Qiagen, Valencia, CA, U.S.A.).

    Transferring:

    Article Title: PARP1 Is Up-Regulated in Non-small Cell Lung Cancer Tissues in the Presence of the Cyanobacterial Toxin Microcystin
    Article Snippet: Nucleic acid free reagents and aerosol resistant pipette tips were used. .. For the FFPE samples, total DNA was isolated from samples using AllPrep DNA/RNA FFPE kit (Qiagen) and QIAamp DNA microbiome kit (Qiagen) following manufacturer's protocol with modifications.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Concurrent leukoencephalomyelitis and polyneuritis in a Maltese terrier: resembling combined central and peripheral demyelination in humans
    Article Snippet: DNA and RNA were extracted from the formalin-fixed, paraffin-embedded cerebrum, cerebellum, and sciatic nerves using an AllPrep DNA/RNA FFPE kit, (Qiagen, Valencia, CA, U.S.A.). .. Polymerase chain reaction (PCR) and reverse transcription PCR (RT-PCR) using primer sets targeting dengue virus [ ], Japanese encephalitis virus [ ], Zika virus [ ], canine distemper virus [ ], rabies virus [ ], pseudorabies virus [ ], T. gondii [ ], N. caninum [ ], Leishmania spp.

    Sequencing:

    Article Title: A new species of Xenoturbella from the western Pacific Ocean and the evolution of Xenoturbella
    Article Snippet: Paragraph title: DNA extraction and sequencing ... Genomic DNA of the X. japonica paratype specimen was extracted using Allprep DNA/RNA FFPE Kit (QIAGEN).

    Multiplexing:

    Article Title: A new species of Xenoturbella from the western Pacific Ocean and the evolution of Xenoturbella
    Article Snippet: Barcode sequences were added to 5′ end of the specific primers, and the PCR products were sequenced by Macrogen Japan using PacBio RS II Multiplexing Targeted Sequencing. .. Genomic DNA of the X. japonica paratype specimen was extracted using Allprep DNA/RNA FFPE Kit (QIAGEN).

    Methylation:

    Article Title: Random forest-based modelling to detect biomarkers for prostate cancer progression
    Article Snippet: Three tissue punches (0.6 mm × 3 mm) were taken of each tissue block, and genomic DNA was isolated using the AllPrep® DNA/RNA FFPE kit (Qiagen). .. DNA was submitted to the DKFZ Genome and Proteome core facility for Illumina 450k Methylation analyses.

    Isolation:

    Article Title: Three Dimensional Culture of Human Renal Cell Carcinoma Organoids
    Article Snippet: .. Construct Molecular Analysis DNA and RNA were isolated from FFPE sections using the AllPrep DNA/RNA FFPE kit (Qiagen). .. Isolated RNA was treated with DNase I (Qiagen) to eliminate potential DNA contamination prior to downstream analysis. cDNA was synthesized using random primers (Ambion, Life Technologies) and the Sensiscript Reverse Transcriptase kit (Qiagen).

    Article Title: Random forest-based modelling to detect biomarkers for prostate cancer progression
    Article Snippet: .. Three tissue punches (0.6 mm × 3 mm) were taken of each tissue block, and genomic DNA was isolated using the AllPrep® DNA/RNA FFPE kit (Qiagen). .. DNA was submitted to the DKFZ Genome and Proteome core facility for Illumina 450k Methylation analyses.

    Article Title: Enzyme-free digital counting of endogenous circular RNA molecules in B-cell malignancies
    Article Snippet: .. For RNA isolation of FFPE cell lines, the Allprep DNA/RNA FFPE kit (Qiagen) was used following a xylene deparaffinization. .. RNA from fresh frozen patient samples was isolated with the RNeasy mini kit (Qiagen).

    Article Title: PARP1 Is Up-Regulated in Non-small Cell Lung Cancer Tissues in the Presence of the Cyanobacterial Toxin Microcystin
    Article Snippet: .. For the FFPE samples, total DNA was isolated from samples using AllPrep DNA/RNA FFPE kit (Qiagen) and QIAamp DNA microbiome kit (Qiagen) following manufacturer's protocol with modifications. .. For the fresh frozen samples, DNA, RNA and protein were extracted using Qiagen's AllPrep DNA/RNA/Protein Mini Kit (Orloff et al., ) (Qiagen, Valencia, CA, USA) according to manufacturer's protocol.

    Article Title: Association between the expression of microRNAs and the response of patients with locally advanced rectal cancer to preoperative chemoradiotherapy
    Article Snippet: .. RNA extraction Total RNA was isolated from the FFPE tissue using the miRNeasy FFPE kit for the test study and the AllPrep DNA/RNA FFPE kit (both Qiagen GmbH, Hilden, Germany) for the validation study according to the manufacturer's protocol. ..

    Article Title: TIAM1 promotes chemoresistance and tumor invasiveness in colorectal cancer
    Article Snippet: .. RNA isolation and quantitative reverse-transcription PCR RNA extraction from tissue samples was performed using the miRNeasy RNA isolation kit (Qiagen, Valencia, California, USA) and AllPrep DNA/RNA FFPE kit (Qiagen) following the manufacturer’s protocol. .. The synthesis of complementary DNA (cDNA) was performed from 500 ng of total RNA using the High Capacity cDNA Reverse-Transcription Kit (Invitrogen, Carlsbad, CA, USA).

    Size-exclusion Chromatography:

    Article Title: Concurrent leukoencephalomyelitis and polyneuritis in a Maltese terrier: resembling combined central and peripheral demyelination in humans
    Article Snippet: After exposure to 2% diaminobenzidine for 3 min, the slides were counterstained with hematoxylin for 30 sec. IHC stainings for CD11d (diluted 1:50; clone CA18.3C6; P.F. .. DNA and RNA were extracted from the formalin-fixed, paraffin-embedded cerebrum, cerebellum, and sciatic nerves using an AllPrep DNA/RNA FFPE kit, (Qiagen, Valencia, CA, U.S.A.).

    Purification:

    Article Title: Increased FGF19 copy number is frequently detected in hepatocellular carcinoma with a complete response after sorafenib treatment
    Article Snippet: .. The DNA was purified using an Allprep DNA/RNA FFPE kit (Qiagen, Valencia, CA) according to the manufacturer's instructions. .. The quality and quantity of the DNA were verified using the NanoDrop 2000 device (Thermo Scientific, Wilmington, DE) and the PicoGreen dsDNA assay kit (Life Technologies, Foster City, CA).

    Polymerase Chain Reaction:

    Article Title: Concurrent leukoencephalomyelitis and polyneuritis in a Maltese terrier: resembling combined central and peripheral demyelination in humans
    Article Snippet: DNA and RNA were extracted from the formalin-fixed, paraffin-embedded cerebrum, cerebellum, and sciatic nerves using an AllPrep DNA/RNA FFPE kit, (Qiagen, Valencia, CA, U.S.A.). .. Polymerase chain reaction (PCR) and reverse transcription PCR (RT-PCR) using primer sets targeting dengue virus [ ], Japanese encephalitis virus [ ], Zika virus [ ], canine distemper virus [ ], rabies virus [ ], pseudorabies virus [ ], T. gondii [ ], N. caninum [ ], Leishmania spp.

    Article Title: Three Dimensional Culture of Human Renal Cell Carcinoma Organoids
    Article Snippet: Construct Molecular Analysis DNA and RNA were isolated from FFPE sections using the AllPrep DNA/RNA FFPE kit (Qiagen). .. PCR for selected markers was carried out with appropriate SYBR or Taqman assay protocols as described above.

    Article Title: A new species of Xenoturbella from the western Pacific Ocean and the evolution of Xenoturbella
    Article Snippet: PCR conditions (taq-polymerases, primers and amplification parameters) are shown in Additional file : Table S4. .. Genomic DNA of the X. japonica paratype specimen was extracted using Allprep DNA/RNA FFPE Kit (QIAGEN).

    Article Title: PARP1 Is Up-Regulated in Non-small Cell Lung Cancer Tissues in the Presence of the Cyanobacterial Toxin Microcystin
    Article Snippet: For the FFPE samples, total DNA was isolated from samples using AllPrep DNA/RNA FFPE kit (Qiagen) and QIAamp DNA microbiome kit (Qiagen) following manufacturer's protocol with modifications. .. The availability of microbial DNA was verified by PCR amplification using 16S rRNA specific primers, 27F (5′-AGAGTTTGATCMTGGCTCAG-3′) and 519R (5′-GWA TTA CCG CGG CKG CTG-3′).

    Article Title: TIAM1 promotes chemoresistance and tumor invasiveness in colorectal cancer
    Article Snippet: .. RNA isolation and quantitative reverse-transcription PCR RNA extraction from tissue samples was performed using the miRNeasy RNA isolation kit (Qiagen, Valencia, California, USA) and AllPrep DNA/RNA FFPE kit (Qiagen) following the manufacturer’s protocol. .. The synthesis of complementary DNA (cDNA) was performed from 500 ng of total RNA using the High Capacity cDNA Reverse-Transcription Kit (Invitrogen, Carlsbad, CA, USA).

    Construct:

    Article Title: Three Dimensional Culture of Human Renal Cell Carcinoma Organoids
    Article Snippet: .. Construct Molecular Analysis DNA and RNA were isolated from FFPE sections using the AllPrep DNA/RNA FFPE kit (Qiagen). .. Isolated RNA was treated with DNase I (Qiagen) to eliminate potential DNA contamination prior to downstream analysis. cDNA was synthesized using random primers (Ambion, Life Technologies) and the Sensiscript Reverse Transcriptase kit (Qiagen).

    TaqMan Assay:

    Article Title: Three Dimensional Culture of Human Renal Cell Carcinoma Organoids
    Article Snippet: Construct Molecular Analysis DNA and RNA were isolated from FFPE sections using the AllPrep DNA/RNA FFPE kit (Qiagen). .. PCR for selected markers was carried out with appropriate SYBR or Taqman assay protocols as described above.

    RNA Extraction:

    Article Title: Molecular Diagnosis Using Residual Liquid-Based Cytology Materials for Patients with Nondiagnostic or Indeterminate Thyroid Nodules
    Article Snippet: Paragraph title: DNA and RNA extraction from residual SurePath material ... DNAs and RNAs were extracted from the samples using the AllPrep DNA/RNA FFPE Kit (Qiagen, Hilden, Germany).

    Article Title: Ectopic expression of PLC‐β2 in non‐invasive breast tumor cells plays a protective role against malignant progression and is correlated with the deregulation of miR‐146a, et al. Ectopic expression of PLC‐β2 in non‐invasive breast tumor cells plays a protective role against malignant progression and is correlated with the deregulation of miR‐146a
    Article Snippet: .. Dissected areas were processed for RNA extraction using AllPrep DNA/RNA FFPE Kit (QIAGEN), following the manufacturer's instructions. .. The short RNA was profiled using an RNAseq approach on SOLiD (Thermo Fisher Scientific, Paisley, UK), following a previously described procedure.

    Article Title: Association between the expression of microRNAs and the response of patients with locally advanced rectal cancer to preoperative chemoradiotherapy
    Article Snippet: .. RNA extraction Total RNA was isolated from the FFPE tissue using the miRNeasy FFPE kit for the test study and the AllPrep DNA/RNA FFPE kit (both Qiagen GmbH, Hilden, Germany) for the validation study according to the manufacturer's protocol. ..

    Article Title: Analytical Validation of the Next-Generation Sequencing Assay for a Nationwide Signal-Finding Clinical Trial
    Article Snippet: Paragraph title: DNA and RNA Extraction and Preparation ... Nucleic acid samples used in other assessments and the fit-for-purpose study (and subsequently for the NCI-MATCH trial tumor specimen) were extracted by Qiagen AllPrep DNA/RNA FFPE kit.

    Article Title: TIAM1 promotes chemoresistance and tumor invasiveness in colorectal cancer
    Article Snippet: .. RNA isolation and quantitative reverse-transcription PCR RNA extraction from tissue samples was performed using the miRNeasy RNA isolation kit (Qiagen, Valencia, California, USA) and AllPrep DNA/RNA FFPE kit (Qiagen) following the manufacturer’s protocol. .. The synthesis of complementary DNA (cDNA) was performed from 500 ng of total RNA using the High Capacity cDNA Reverse-Transcription Kit (Invitrogen, Carlsbad, CA, USA).

    Selection:

    Article Title: Random forest-based modelling to detect biomarkers for prostate cancer progression
    Article Snippet: Sample selection was based on the following criteria: good prognosis indicated by the presence of organ-confined disease (pT2) and lack of biochemical prostate-specific antigen (PSA)-based recurrence (BCR) for at least 5 years. .. Three tissue punches (0.6 mm × 3 mm) were taken of each tissue block, and genomic DNA was isolated using the AllPrep® DNA/RNA FFPE kit (Qiagen).

    Next-Generation Sequencing:

    Article Title: Analytical Validation of the Next-Generation Sequencing Assay for a Nationwide Signal-Finding Clinical Trial
    Article Snippet: Nucleic acid samples used in other assessments and the fit-for-purpose study (and subsequently for the NCI-MATCH trial tumor specimen) were extracted by Qiagen AllPrep DNA/RNA FFPE kit. .. All nucleic acid samples were quantitated by Qubit fluorometer (Thermo Fisher Scientific) before use in the NGS assay.

    Spectrophotometry:

    Article Title: PARP1 Is Up-Regulated in Non-small Cell Lung Cancer Tissues in the Presence of the Cyanobacterial Toxin Microcystin
    Article Snippet: For the FFPE samples, total DNA was isolated from samples using AllPrep DNA/RNA FFPE kit (Qiagen) and QIAamp DNA microbiome kit (Qiagen) following manufacturer's protocol with modifications. .. The extracted DNA was quantified using the NanoDrop Lite spectrophotometer (Thermo Scientific), which ranged from 30 to 98 ng/μl.

    DNA Methylation Assay:

    Article Title: Random forest-based modelling to detect biomarkers for prostate cancer progression
    Article Snippet: Three tissue punches (0.6 mm × 3 mm) were taken of each tissue block, and genomic DNA was isolated using the AllPrep® DNA/RNA FFPE kit (Qiagen). .. After removing samples and DNA methylation profiles with low quality, the study included 35 patients with good and 35 patients with bad prognosis (Additional file : Table S1).

    CTG Assay:

    Article Title: PARP1 Is Up-Regulated in Non-small Cell Lung Cancer Tissues in the Presence of the Cyanobacterial Toxin Microcystin
    Article Snippet: For the FFPE samples, total DNA was isolated from samples using AllPrep DNA/RNA FFPE kit (Qiagen) and QIAamp DNA microbiome kit (Qiagen) following manufacturer's protocol with modifications. .. The availability of microbial DNA was verified by PCR amplification using 16S rRNA specific primers, 27F (5′-AGAGTTTGATCMTGGCTCAG-3′) and 519R (5′-GWA TTA CCG CGG CKG CTG-3′).

    Staining:

    Article Title: Increased FGF19 copy number is frequently detected in hepatocellular carcinoma with a complete response after sorafenib treatment
    Article Snippet: DNA extraction All FFPE specimens underwent a histological review, and only those containing sufficient tumor cells (at least 70% tumor cells) as revealed using hematoxylin-eosin staining were subjected to DNA extraction. .. The DNA was purified using an Allprep DNA/RNA FFPE kit (Qiagen, Valencia, CA) according to the manufacturer's instructions.

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    Qiagen allprep dna rna mirna universal kit
    Allprep Dna Rna Mirna Universal Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/allprep dna rna mirna universal kit/product/Qiagen
    Average 90 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    allprep dna rna mirna universal kit - by Bioz Stars, 2020-01
    90/100 stars
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