allprep dna rna mini kit 80204  (Qiagen)


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    AllPrep DNA RNA Mini Kit
    Description:
    For simultaneous purification of DNA and RNA from cells and tissues Kit contents Qiagen AllPrep DNA RNA Mini Kit 50 preps 30mg Sample 100L Elution Volume Silica Technology Spin Column Format Manual Processing Genomic DNA Total RNA Purification 35 min Time Run Ideal for PCR Real time PCR Microarray Blotting For Simultaneous Purification of DNA and RNA from Cells and Tissues Includes AllPrep DNA Spin Columns RNeasy Mini Spin Columns Collection Tubes RNase free Water and Buffers Benefits High quality DNA and RNA from the same sample Maximal yields of DNA and RNA from precious samples Rapid purification with short streamlined protocol Ready to use DNA and RNA for any downstream analysis
    Catalog Number:
    80204
    Price:
    541
    Category:
    AllPrep DNA RNA Mini Kit
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    Structured Review

    Qiagen allprep dna rna mini kit 80204
    AllPrep DNA RNA Mini Kit
    For simultaneous purification of DNA and RNA from cells and tissues Kit contents Qiagen AllPrep DNA RNA Mini Kit 50 preps 30mg Sample 100L Elution Volume Silica Technology Spin Column Format Manual Processing Genomic DNA Total RNA Purification 35 min Time Run Ideal for PCR Real time PCR Microarray Blotting For Simultaneous Purification of DNA and RNA from Cells and Tissues Includes AllPrep DNA Spin Columns RNeasy Mini Spin Columns Collection Tubes RNase free Water and Buffers Benefits High quality DNA and RNA from the same sample Maximal yields of DNA and RNA from precious samples Rapid purification with short streamlined protocol Ready to use DNA and RNA for any downstream analysis
    https://www.bioz.com/result/allprep dna rna mini kit 80204/product/Qiagen
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    allprep dna rna mini kit 80204 - by Bioz Stars, 2020-01
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    Related Articles

    Methylation Sequencing:

    Article Title: Epigenetic programming underpins B cell dysfunction in human SLE
    Article Snippet: Paragraph title: Reduced representation bisulfite sequencing (RRBS) ... DNA was isolated from the same fraction as RNA using the AllPrep DNA/RNA Mini Kit (Qiagen) according to the manufacturer’s instructions.

    Centrifugation:

    Article Title: Genome-Wide Characterization of Pancreatic Adenocarcinoma Patients Using Next Generation Sequencing
    Article Snippet: Genomic DNA isolation Tissue was disrupted and homogenized in Buffer RLT plus (Qiagen AllPrep DNA/RNA Mini Kit), using the Bullet Blender™, Next Advance, and transferred to a microcentrifuge tube containing Buffer RLT plus and 1.6 mm stainless steel beads (patient 1), or 0.9 mm–2.0 mm RNase free stainless steel beads (patients 2 and 3). .. Blood leukocytes (buffy coat) were isolated from whole blood by centrifugation at room temperature and resuspended in Buffer RLT plus.

    Amplification:

    Article Title: Longitudinal study of the bacterial and fungal microbiota in the human sinuses reveals seasonal and annual changes in diversity
    Article Snippet: .. DNA collection and target gene amplification Samples were thawed on ice, and DNA was extracted from pairs of swabs using the Qiagen® AllPrep DNA/RNA Mini Kit (Bio-Strategy Ltd, Auckland, New Zealand) as previously described . .. Elution Buffer EB (55 µL) was added to the spin column filter and incubated for 5 min before DNA was eluted by centrifuging for 1 min at 11,200 x g. The eluate was centrifuged through the spin column filter a second time to increase DNA concentration.

    Synthesized:

    Article Title: Dissociable Deficits of Executive Function Caused by Gestational Adversity are Linked to Specific Transcriptional Changes in the Prefrontal Cortex
    Article Snippet: The PFC (consisting of anterior cingulate, prelimbic, and infralimbic) was dissected from a 2-mm coronal slice from bregma +2.3 to +0.3, and DNA and RNA was extracted using Qiagen AllPrep DNA/RNA Mini kit. .. Hundred μg/μl cDNA was synthesized using Applied Biosystems High Capacity Reverse Transcriptase kit.

    Cell Isolation:

    Article Title: Fimepinostat, a novel dual inhibitor of HDAC and PI3K, effectively reverses HIV-1 latency ex vivo without T cell activation
    Article Snippet: CD4+ T cells were enriched from thawed PBMCs from donors living with HIV-1 using a CD4+ T cell isolation kit and magnetic-activated-cell-sorting (MACS) columns using negative selection (catalogue no. 130-096-533, Miltenyi Biotec, Germany) according to the manufacturer's instructions. .. After 16 hours of incubation at 37°C with 5% CO2 , cells were harvested, lysed in RLT+ buffer and stored at −80°C until RNA was extracted using AllPrep DNA/RNA Mini Kit (Qiagen, Germany) according to the manufacturer's instructions.

    Quantitative RT-PCR:

    Article Title: Transcriptome analysis reveals significant differences between primary plasma cell leukemia and multiple myeloma even when sharing a similar genetic background
    Article Snippet: Nucleic acid extraction, quantitative real-time PCR and TP53 mutation analysis RNA and DNA were extracted using AllPrep DNA/RNA Mini Kit (Qiagen). .. Gene expression was evaluated with TaqMan (Applied Biosystems) qRT-PCR assays, and isoform and exon expression were evaluated using SYBR Green (Bio-Rad) qRT-PCR mRNA assays.

    SYBR Green Assay:

    Article Title: Transcriptome analysis reveals significant differences between primary plasma cell leukemia and multiple myeloma even when sharing a similar genetic background
    Article Snippet: Nucleic acid extraction, quantitative real-time PCR and TP53 mutation analysis RNA and DNA were extracted using AllPrep DNA/RNA Mini Kit (Qiagen). .. Gene expression was evaluated with TaqMan (Applied Biosystems) qRT-PCR assays, and isoform and exon expression were evaluated using SYBR Green (Bio-Rad) qRT-PCR mRNA assays.

    Incubation:

    Article Title: Fimepinostat, a novel dual inhibitor of HDAC and PI3K, effectively reverses HIV-1 latency ex vivo without T cell activation
    Article Snippet: .. After 16 hours of incubation at 37°C with 5% CO2 , cells were harvested, lysed in RLT+ buffer and stored at −80°C until RNA was extracted using AllPrep DNA/RNA Mini Kit (Qiagen, Germany) according to the manufacturer's instructions. .. Cell-associated unspliced HIV-1 (CA usHIV-1) RNA was measured using the QX100 Droplet Digital PCR System (BioRad Inc, USA) as described previously .

    Article Title: Longitudinal study of the bacterial and fungal microbiota in the human sinuses reveals seasonal and annual changes in diversity
    Article Snippet: DNA collection and target gene amplification Samples were thawed on ice, and DNA was extracted from pairs of swabs using the Qiagen® AllPrep DNA/RNA Mini Kit (Bio-Strategy Ltd, Auckland, New Zealand) as previously described . .. Elution Buffer EB (55 µL) was added to the spin column filter and incubated for 5 min before DNA was eluted by centrifuging for 1 min at 11,200 x g. The eluate was centrifuged through the spin column filter a second time to increase DNA concentration.

    Formalin-fixed Paraffin-Embedded:

    Article Title: Cell‐of‐origin determined by both gene expression profiling and immunohistochemistry is the strongest predictor of survival in patients with diffuse large B‐cell lymphoma, et al. Cell‐of‐origin determined by both gene expression profiling and immunohistochemistry is the strongest predictor of survival in patients with diffuse large B‐cell lymphoma
    Article Snippet: .. 2.2 RNA extraction Extraction of RNA from FFPE tissue was done according to the AllPrep DNA/RNA Mini Kit for FFPE protocol (Qiagen, Hilden, Germany). .. That protocol allows for the simultaneous purification of genomic DNA and total RNA from the same biological sample.

    Article Title: Region-specific glucocorticoid receptor promoter methylation has both positive and negative prognostic value in patients with estrogen receptor-positive breast cancer
    Article Snippet: The isolation of DNA from MCF-7 and T47-D FFPE cell pellets was performed using the same methods as the MA.12 samples, outlined above. .. Briefly, tumor samples were completely homogenized, and DNA extraction was carried out using the AllPrep DNA/RNA Mini Kit (Qiagen).

    Expressing:

    Article Title: Transcriptome analysis reveals significant differences between primary plasma cell leukemia and multiple myeloma even when sharing a similar genetic background
    Article Snippet: Nucleic acid extraction, quantitative real-time PCR and TP53 mutation analysis RNA and DNA were extracted using AllPrep DNA/RNA Mini Kit (Qiagen). .. Gene expression was evaluated with TaqMan (Applied Biosystems) qRT-PCR assays, and isoform and exon expression were evaluated using SYBR Green (Bio-Rad) qRT-PCR mRNA assays.

    Article Title: Dissociable Deficits of Executive Function Caused by Gestational Adversity are Linked to Specific Transcriptional Changes in the Prefrontal Cortex
    Article Snippet: Paragraph title: Gene expression in Prefrontal Cortex ... The PFC (consisting of anterior cingulate, prelimbic, and infralimbic) was dissected from a 2-mm coronal slice from bregma +2.3 to +0.3, and DNA and RNA was extracted using Qiagen AllPrep DNA/RNA Mini kit.

    Article Title: Transcription Driven Somatic DNA Methylation within the Imprinted Gnas Cluster
    Article Snippet: Total RNA was extracted from newborn brain with the Allprep DNA/RNA Mini Kit (Qiagen) and from Brown Adipose Tissue (BAT) with an RNAeasy lipid kit (Qiagen), followed by reverse transcription with the High Capacity cDNA Reverse Transcription kit (Applied Biosystems). .. Each reaction contained 1x pre-assembled Taqman gene expression assay (a transcript specific FAM dye labelled TaqMan MGB probe and an unlabelled primer set), 1x TaqMan Fast Universal PCR Master Mix (Applied Biosystems) and 50 ng of cDNA, and was performed in triplicate on a 7500 Fast Real-Time PCR machine.

    Flow Cytometry:

    Article Title: Cell‐of‐origin determined by both gene expression profiling and immunohistochemistry is the strongest predictor of survival in patients with diffuse large B‐cell lymphoma, et al. Cell‐of‐origin determined by both gene expression profiling and immunohistochemistry is the strongest predictor of survival in patients with diffuse large B‐cell lymphoma
    Article Snippet: 2.2 RNA extraction Extraction of RNA from FFPE tissue was done according to the AllPrep DNA/RNA Mini Kit for FFPE protocol (Qiagen, Hilden, Germany). .. Purification of RNA was done with the AllPrep column flow‐through, using an RNeasy Mini spin column.

    Digital PCR:

    Article Title: Fimepinostat, a novel dual inhibitor of HDAC and PI3K, effectively reverses HIV-1 latency ex vivo without T cell activation
    Article Snippet: After 16 hours of incubation at 37°C with 5% CO2 , cells were harvested, lysed in RLT+ buffer and stored at −80°C until RNA was extracted using AllPrep DNA/RNA Mini Kit (Qiagen, Germany) according to the manufacturer's instructions. .. Cell-associated unspliced HIV-1 (CA usHIV-1) RNA was measured using the QX100 Droplet Digital PCR System (BioRad Inc, USA) as described previously .

    Article Title: HIV Impairs Lung Epithelial Integrity and Enters the Epithelium to Promote Chronic Lung Inflammation
    Article Snippet: .. Cell-associated RNA and DNA were extracted from these frozen cells using AllPrep DNA/RNA Mini Kit (Qiagen, Valencia, CA) and quantified by droplet digital PCR [ ]. .. Media p24 antigen levels were quantified using Alliance HIV ELISA Kit (PerkinElmer).

    Polymerase Chain Reaction:

    Article Title: Longitudinal study of the bacterial and fungal microbiota in the human sinuses reveals seasonal and annual changes in diversity
    Article Snippet: DNA collection and target gene amplification Samples were thawed on ice, and DNA was extracted from pairs of swabs using the Qiagen® AllPrep DNA/RNA Mini Kit (Bio-Strategy Ltd, Auckland, New Zealand) as previously described . .. Triplicate negative extractions and PCR amplification of PCR-grade water were performed to test the DNA extraction kits for contamination via PCR.

    Article Title: Transcription Driven Somatic DNA Methylation within the Imprinted Gnas Cluster
    Article Snippet: Total RNA was extracted from newborn brain with the Allprep DNA/RNA Mini Kit (Qiagen) and from Brown Adipose Tissue (BAT) with an RNAeasy lipid kit (Qiagen), followed by reverse transcription with the High Capacity cDNA Reverse Transcription kit (Applied Biosystems). .. Each reaction contained 1x pre-assembled Taqman gene expression assay (a transcript specific FAM dye labelled TaqMan MGB probe and an unlabelled primer set), 1x TaqMan Fast Universal PCR Master Mix (Applied Biosystems) and 50 ng of cDNA, and was performed in triplicate on a 7500 Fast Real-Time PCR machine.

    Article Title: Activation of p53 by Nutlin-3a Induces Apoptosis and Cellular Senescence in Human Glioblastoma Multiforme
    Article Snippet: .. Methylation-specific PCR Genomic DNA was isolated from frozen tumor using the AllPrep DNA/RNA Mini Kit (Quiagen). .. DNA methylation status of CpG islands at the enzyme O6 -methylguanine methyltransferase (MGMT) promoter was determined by methylation-specific PCR (MSP), as previously described , with some modifications.

    Magnetic Cell Separation:

    Article Title: Fimepinostat, a novel dual inhibitor of HDAC and PI3K, effectively reverses HIV-1 latency ex vivo without T cell activation
    Article Snippet: CD4+ T cells were enriched from thawed PBMCs from donors living with HIV-1 using a CD4+ T cell isolation kit and magnetic-activated-cell-sorting (MACS) columns using negative selection (catalogue no. 130-096-533, Miltenyi Biotec, Germany) according to the manufacturer's instructions. .. After 16 hours of incubation at 37°C with 5% CO2 , cells were harvested, lysed in RLT+ buffer and stored at −80°C until RNA was extracted using AllPrep DNA/RNA Mini Kit (Qiagen, Germany) according to the manufacturer's instructions.

    DNA Extraction:

    Article Title: Genome-Wide Characterization of Pancreatic Adenocarcinoma Patients Using Next Generation Sequencing
    Article Snippet: .. Genomic DNA isolation Tissue was disrupted and homogenized in Buffer RLT plus (Qiagen AllPrep DNA/RNA Mini Kit), using the Bullet Blender™, Next Advance, and transferred to a microcentrifuge tube containing Buffer RLT plus and 1.6 mm stainless steel beads (patient 1), or 0.9 mm–2.0 mm RNase free stainless steel beads (patients 2 and 3). .. Blood leukocytes (buffy coat) were isolated from whole blood by centrifugation at room temperature and resuspended in Buffer RLT plus.

    Article Title: Longitudinal study of the bacterial and fungal microbiota in the human sinuses reveals seasonal and annual changes in diversity
    Article Snippet: DNA collection and target gene amplification Samples were thawed on ice, and DNA was extracted from pairs of swabs using the Qiagen® AllPrep DNA/RNA Mini Kit (Bio-Strategy Ltd, Auckland, New Zealand) as previously described . .. Triplicate negative extractions and PCR amplification of PCR-grade water were performed to test the DNA extraction kits for contamination via PCR.

    Article Title: Region-specific glucocorticoid receptor promoter methylation has both positive and negative prognostic value in patients with estrogen receptor-positive breast cancer
    Article Snippet: .. Briefly, tumor samples were completely homogenized, and DNA extraction was carried out using the AllPrep DNA/RNA Mini Kit (Qiagen). .. All DNA samples in this study were bisulfite converted using the EpiTect Fast DNA Bisulfite Kit (Qiagen) following the manufacturer’s protocol.

    Article Title: Mutational landscape in genetically engineered, carcinogen-induced, and radiation-induced mouse sarcoma
    Article Snippet: .. DNA extraction was performed using DNeasy Blood and Tissue Kit or AllPrep DNA/RNA Mini Kit (Qiagen). ..

    Methylation:

    Article Title: Comprehensive analysis of genome-wide DNA methylation across human polycystic ovary syndrome ovary granulosa cell
    Article Snippet: .. Granulosa cell DNA/RNA extraction and global methylation/hydroxymethylation quantification The GCs DNA and RNA were extracted using the AllPrep DNA/RNA Mini Kit (Qiagen, Hilden, Germany). ..

    Article Title: Epigenetic programming underpins B cell dysfunction in human SLE
    Article Snippet: DNA was isolated from the same fraction as RNA using the AllPrep DNA/RNA Mini Kit (Qiagen) according to the manufacturer’s instructions. .. For bisulfite conversion controls, ΦX174 phage DNA (NEB) was in vitro methylated with M.SssI methylase (NEB).

    Article Title: Activation of p53 by Nutlin-3a Induces Apoptosis and Cellular Senescence in Human Glioblastoma Multiforme
    Article Snippet: .. Methylation-specific PCR Genomic DNA was isolated from frozen tumor using the AllPrep DNA/RNA Mini Kit (Quiagen). .. DNA methylation status of CpG islands at the enzyme O6 -methylguanine methyltransferase (MGMT) promoter was determined by methylation-specific PCR (MSP), as previously described , with some modifications.

    Article Title: Divergent phenotypes of human regulatory T cells expressing the receptors TIGIT and CD226
    Article Snippet: Nucleotides were isolated with AllPrep DNA/RNA Mini Kit (Qiagen) or DNeasy tissue kit (Qiagen), as appropriate. .. Real-time PCR was performed to determine the methylation status at the FOXP3-TSDR for each sample.

    Mutagenesis:

    Article Title: Transcriptome analysis reveals significant differences between primary plasma cell leukemia and multiple myeloma even when sharing a similar genetic background
    Article Snippet: .. Nucleic acid extraction, quantitative real-time PCR and TP53 mutation analysis RNA and DNA were extracted using AllPrep DNA/RNA Mini Kit (Qiagen). .. RNA integrity was assessed using Agilent 2100 Bioanalyzer.

    Isolation:

    Article Title: Genome-Wide Characterization of Pancreatic Adenocarcinoma Patients Using Next Generation Sequencing
    Article Snippet: Genomic DNA isolation Tissue was disrupted and homogenized in Buffer RLT plus (Qiagen AllPrep DNA/RNA Mini Kit), using the Bullet Blender™, Next Advance, and transferred to a microcentrifuge tube containing Buffer RLT plus and 1.6 mm stainless steel beads (patient 1), or 0.9 mm–2.0 mm RNase free stainless steel beads (patients 2 and 3). .. Blood leukocytes (buffy coat) were isolated from whole blood by centrifugation at room temperature and resuspended in Buffer RLT plus.

    Article Title: Fimepinostat, a novel dual inhibitor of HDAC and PI3K, effectively reverses HIV-1 latency ex vivo without T cell activation
    Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated using SepMate-50 (STEMCELL Technologies, Canada) with Ficoll-Paque (GE Healthcare, Denmark) density separation according to the manufacturers’ instructions. .. After 16 hours of incubation at 37°C with 5% CO2 , cells were harvested, lysed in RLT+ buffer and stored at −80°C until RNA was extracted using AllPrep DNA/RNA Mini Kit (Qiagen, Germany) according to the manufacturer's instructions.

    Article Title: Region-specific glucocorticoid receptor promoter methylation has both positive and negative prognostic value in patients with estrogen receptor-positive breast cancer
    Article Snippet: The isolation of DNA from MCF-7 and T47-D FFPE cell pellets was performed using the same methods as the MA.12 samples, outlined above. .. Briefly, tumor samples were completely homogenized, and DNA extraction was carried out using the AllPrep DNA/RNA Mini Kit (Qiagen).

    Article Title: Epigenetic programming underpins B cell dysfunction in human SLE
    Article Snippet: .. DNA was isolated from the same fraction as RNA using the AllPrep DNA/RNA Mini Kit (Qiagen) according to the manufacturer’s instructions. .. A dual restriction enzyme RRBS assay was performed by splitting the DNA into two aliquots and digesting over night with either MspI or TaqI (NEB).

    Article Title: Activation of p53 by Nutlin-3a Induces Apoptosis and Cellular Senescence in Human Glioblastoma Multiforme
    Article Snippet: .. Methylation-specific PCR Genomic DNA was isolated from frozen tumor using the AllPrep DNA/RNA Mini Kit (Quiagen). .. DNA methylation status of CpG islands at the enzyme O6 -methylguanine methyltransferase (MGMT) promoter was determined by methylation-specific PCR (MSP), as previously described , with some modifications.

    Article Title: Divergent phenotypes of human regulatory T cells expressing the receptors TIGIT and CD226
    Article Snippet: .. Nucleotides were isolated with AllPrep DNA/RNA Mini Kit (Qiagen) or DNeasy tissue kit (Qiagen), as appropriate. .. Bisulfite treatment of genomic DNA was performed on 500ng of DNA with the EZ DNA Methylation Kit (Zymo Research).

    Purification:

    Article Title: Genome-Wide Characterization of Pancreatic Adenocarcinoma Patients Using Next Generation Sequencing
    Article Snippet: Genomic DNA isolation Tissue was disrupted and homogenized in Buffer RLT plus (Qiagen AllPrep DNA/RNA Mini Kit), using the Bullet Blender™, Next Advance, and transferred to a microcentrifuge tube containing Buffer RLT plus and 1.6 mm stainless steel beads (patient 1), or 0.9 mm–2.0 mm RNase free stainless steel beads (patients 2 and 3). .. Genomic DNA was purified following the manufacturer's protocol.

    Article Title: Cell‐of‐origin determined by both gene expression profiling and immunohistochemistry is the strongest predictor of survival in patients with diffuse large B‐cell lymphoma, et al. Cell‐of‐origin determined by both gene expression profiling and immunohistochemistry is the strongest predictor of survival in patients with diffuse large B‐cell lymphoma
    Article Snippet: 2.2 RNA extraction Extraction of RNA from FFPE tissue was done according to the AllPrep DNA/RNA Mini Kit for FFPE protocol (Qiagen, Hilden, Germany). .. That protocol allows for the simultaneous purification of genomic DNA and total RNA from the same biological sample.

    Article Title: Epigenetic programming underpins B cell dysfunction in human SLE
    Article Snippet: DNA was isolated from the same fraction as RNA using the AllPrep DNA/RNA Mini Kit (Qiagen) according to the manufacturer’s instructions. .. The resulting DNA was purified, and digestion efficiency quantitated by qPCR using primers spanning sequences that contained one, both, or no restriction site.

    RNA Expression:

    Article Title: Aging and estradiol effects on gene expression in the medial preoptic area, bed nucleus of the stria terminalis, and posterodorsal medial amygdala of male rats
    Article Snippet: Paragraph title: 2.2. RNA expression ... RNA was extracted from frozen POA, BnST, and MePD punches using an Allprep DNA/RNA mini-kit (QIAGEN, Valencia, California), according to the manufacturer’s protocols.

    Real-time Polymerase Chain Reaction:

    Article Title: Transcriptome analysis reveals significant differences between primary plasma cell leukemia and multiple myeloma even when sharing a similar genetic background
    Article Snippet: .. Nucleic acid extraction, quantitative real-time PCR and TP53 mutation analysis RNA and DNA were extracted using AllPrep DNA/RNA Mini Kit (Qiagen). .. RNA integrity was assessed using Agilent 2100 Bioanalyzer.

    Article Title: Dissociable Deficits of Executive Function Caused by Gestational Adversity are Linked to Specific Transcriptional Changes in the Prefrontal Cortex
    Article Snippet: The PFC (consisting of anterior cingulate, prelimbic, and infralimbic) was dissected from a 2-mm coronal slice from bregma +2.3 to +0.3, and DNA and RNA was extracted using Qiagen AllPrep DNA/RNA Mini kit. .. Gene expression was assayed using Taqman primers on custom OpenArray real-time PCR plates assaying housekeeping genes (GAPDH, ACTB) and 18 genes of interest ( ).

    Article Title: Epigenetic programming underpins B cell dysfunction in human SLE
    Article Snippet: DNA was isolated from the same fraction as RNA using the AllPrep DNA/RNA Mini Kit (Qiagen) according to the manufacturer’s instructions. .. The resulting DNA was purified, and digestion efficiency quantitated by qPCR using primers spanning sequences that contained one, both, or no restriction site.

    Article Title: Transcription Driven Somatic DNA Methylation within the Imprinted Gnas Cluster
    Article Snippet: Total RNA was extracted from newborn brain with the Allprep DNA/RNA Mini Kit (Qiagen) and from Brown Adipose Tissue (BAT) with an RNAeasy lipid kit (Qiagen), followed by reverse transcription with the High Capacity cDNA Reverse Transcription kit (Applied Biosystems). .. The relative quantification of Gnas , Gnasxl and Exon1A transcripts was carried out by quantitative real time PCR (qPCR).

    Article Title: Divergent phenotypes of human regulatory T cells expressing the receptors TIGIT and CD226
    Article Snippet: Nucleotides were isolated with AllPrep DNA/RNA Mini Kit (Qiagen) or DNeasy tissue kit (Qiagen), as appropriate. .. Real-time PCR was performed to determine the methylation status at the FOXP3-TSDR for each sample.

    RNA Extraction:

    Article Title: Cell‐of‐origin determined by both gene expression profiling and immunohistochemistry is the strongest predictor of survival in patients with diffuse large B‐cell lymphoma, et al. Cell‐of‐origin determined by both gene expression profiling and immunohistochemistry is the strongest predictor of survival in patients with diffuse large B‐cell lymphoma
    Article Snippet: .. 2.2 RNA extraction Extraction of RNA from FFPE tissue was done according to the AllPrep DNA/RNA Mini Kit for FFPE protocol (Qiagen, Hilden, Germany). .. That protocol allows for the simultaneous purification of genomic DNA and total RNA from the same biological sample.

    Selection:

    Article Title: Fimepinostat, a novel dual inhibitor of HDAC and PI3K, effectively reverses HIV-1 latency ex vivo without T cell activation
    Article Snippet: CD4+ T cells were enriched from thawed PBMCs from donors living with HIV-1 using a CD4+ T cell isolation kit and magnetic-activated-cell-sorting (MACS) columns using negative selection (catalogue no. 130-096-533, Miltenyi Biotec, Germany) according to the manufacturer's instructions. .. After 16 hours of incubation at 37°C with 5% CO2 , cells were harvested, lysed in RLT+ buffer and stored at −80°C until RNA was extracted using AllPrep DNA/RNA Mini Kit (Qiagen, Germany) according to the manufacturer's instructions.

    In Vitro:

    Article Title: Epigenetic programming underpins B cell dysfunction in human SLE
    Article Snippet: DNA was isolated from the same fraction as RNA using the AllPrep DNA/RNA Mini Kit (Qiagen) according to the manufacturer’s instructions. .. For bisulfite conversion controls, ΦX174 phage DNA (NEB) was in vitro methylated with M.SssI methylase (NEB).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Comprehensive analysis of genome-wide DNA methylation across human polycystic ovary syndrome ovary granulosa cell
    Article Snippet: Granulosa cell DNA/RNA extraction and global methylation/hydroxymethylation quantification The GCs DNA and RNA were extracted using the AllPrep DNA/RNA Mini Kit (Qiagen, Hilden, Germany). .. Global DNA methylation and hydroxymethylation status were detected using MethylFlashTM Methylated and Hydroxymethylated DNA Quantification Kit (Epigentek, Epigentek Group Inc., USA), both of which are ELISA-like reactions.

    Article Title: HIV Impairs Lung Epithelial Integrity and Enters the Epithelium to Promote Chronic Lung Inflammation
    Article Snippet: Cell-associated RNA and DNA were extracted from these frozen cells using AllPrep DNA/RNA Mini Kit (Qiagen, Valencia, CA) and quantified by droplet digital PCR [ ]. .. Media p24 antigen levels were quantified using Alliance HIV ELISA Kit (PerkinElmer).

    Ethanol Precipitation:

    Article Title: Aging and estradiol effects on gene expression in the medial preoptic area, bed nucleus of the stria terminalis, and posterodorsal medial amygdala of male rats
    Article Snippet: RNA was extracted from frozen POA, BnST, and MePD punches using an Allprep DNA/RNA mini-kit (QIAGEN, Valencia, California), according to the manufacturer’s protocols. .. RNA samples were eluted with nuclease-free water and treated with 1 U of TURBO deoxyribonuclease (Applied Biosystems Inc, Foster City, California) to rid samples of genomic DNA before ethanol precipitation.

    Quantitation Assay:

    Article Title: Region-specific glucocorticoid receptor promoter methylation has both positive and negative prognostic value in patients with estrogen receptor-positive breast cancer
    Article Snippet: Quantitation of DNA yield was performed with a Qiaxpert spectrophotometer (Qiagen), and samples with a minimum DNA concentration of 5 ng/μL (140 ng total yield) were subjected to bisulfite conversion. .. Briefly, tumor samples were completely homogenized, and DNA extraction was carried out using the AllPrep DNA/RNA Mini Kit (Qiagen).

    Article Title: Mutational landscape in genetically engineered, carcinogen-induced, and radiation-induced mouse sarcoma
    Article Snippet: DNA extraction was performed using DNeasy Blood and Tissue Kit or AllPrep DNA/RNA Mini Kit (Qiagen). .. Genomic DNA samples were quantified using fluorometric quantitation on the Qubit 2.0 (Thermo Fisher Scientific).

    Spectrophotometry:

    Article Title: Region-specific glucocorticoid receptor promoter methylation has both positive and negative prognostic value in patients with estrogen receptor-positive breast cancer
    Article Snippet: Quantitation of DNA yield was performed with a Qiaxpert spectrophotometer (Qiagen), and samples with a minimum DNA concentration of 5 ng/μL (140 ng total yield) were subjected to bisulfite conversion. .. Briefly, tumor samples were completely homogenized, and DNA extraction was carried out using the AllPrep DNA/RNA Mini Kit (Qiagen).

    DNA Methylation Assay:

    Article Title: Comprehensive analysis of genome-wide DNA methylation across human polycystic ovary syndrome ovary granulosa cell
    Article Snippet: Granulosa cell DNA/RNA extraction and global methylation/hydroxymethylation quantification The GCs DNA and RNA were extracted using the AllPrep DNA/RNA Mini Kit (Qiagen, Hilden, Germany). .. Global DNA methylation and hydroxymethylation status were detected using MethylFlashTM Methylated and Hydroxymethylated DNA Quantification Kit (Epigentek, Epigentek Group Inc., USA), both of which are ELISA-like reactions.

    Article Title: Activation of p53 by Nutlin-3a Induces Apoptosis and Cellular Senescence in Human Glioblastoma Multiforme
    Article Snippet: Methylation-specific PCR Genomic DNA was isolated from frozen tumor using the AllPrep DNA/RNA Mini Kit (Quiagen). .. DNA methylation status of CpG islands at the enzyme O6 -methylguanine methyltransferase (MGMT) promoter was determined by methylation-specific PCR (MSP), as previously described , with some modifications.

    Article Title: Divergent phenotypes of human regulatory T cells expressing the receptors TIGIT and CD226
    Article Snippet: Nucleotides were isolated with AllPrep DNA/RNA Mini Kit (Qiagen) or DNeasy tissue kit (Qiagen), as appropriate. .. Bisulfite treatment of genomic DNA was performed on 500ng of DNA with the EZ DNA Methylation Kit (Zymo Research).

    Concentration Assay:

    Article Title: Aging and estradiol effects on gene expression in the medial preoptic area, bed nucleus of the stria terminalis, and posterodorsal medial amygdala of male rats
    Article Snippet: RNA was extracted from frozen POA, BnST, and MePD punches using an Allprep DNA/RNA mini-kit (QIAGEN, Valencia, California), according to the manufacturer’s protocols. .. The purity, integrity and concentration of all samples were verified by running them on a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, California) and a nanodrop.

    Article Title: Longitudinal study of the bacterial and fungal microbiota in the human sinuses reveals seasonal and annual changes in diversity
    Article Snippet: DNA collection and target gene amplification Samples were thawed on ice, and DNA was extracted from pairs of swabs using the Qiagen® AllPrep DNA/RNA Mini Kit (Bio-Strategy Ltd, Auckland, New Zealand) as previously described . .. Elution Buffer EB (55 µL) was added to the spin column filter and incubated for 5 min before DNA was eluted by centrifuging for 1 min at 11,200 x g. The eluate was centrifuged through the spin column filter a second time to increase DNA concentration.

    Article Title: Region-specific glucocorticoid receptor promoter methylation has both positive and negative prognostic value in patients with estrogen receptor-positive breast cancer
    Article Snippet: Quantitation of DNA yield was performed with a Qiaxpert spectrophotometer (Qiagen), and samples with a minimum DNA concentration of 5 ng/μL (140 ng total yield) were subjected to bisulfite conversion. .. Briefly, tumor samples were completely homogenized, and DNA extraction was carried out using the AllPrep DNA/RNA Mini Kit (Qiagen).

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    Qiagen allprep dna rna mini kit
    Comparison of sera exosomal <t>RNA</t> using four different RNA extraction methods. (A) Total RNA yield from ultracentrifugation (UC) and ExoQuick (EQ) treated samples using the RNeasy Mini Kit combined with TRIzol LS, the RNeasy Mini Kit alone, conventional RNA precipitation, and <t>AllPrep</t> <t>DNA/RNA</t> Mini Kit. (B) Demonstration of RNA quality measured by OD 260 /OD 280 in EQ and UC treated samples. Data are shown as the mean ± SD from six independent patient samples. *** P
    Allprep Dna Rna Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 174 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/allprep dna rna mini kit/product/Qiagen
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    Comparison of sera exosomal RNA using four different RNA extraction methods. (A) Total RNA yield from ultracentrifugation (UC) and ExoQuick (EQ) treated samples using the RNeasy Mini Kit combined with TRIzol LS, the RNeasy Mini Kit alone, conventional RNA precipitation, and AllPrep DNA/RNA Mini Kit. (B) Demonstration of RNA quality measured by OD 260 /OD 280 in EQ and UC treated samples. Data are shown as the mean ± SD from six independent patient samples. *** P

    Journal: PLoS ONE

    Article Title: Optimizing exosomal RNA isolation for RNA-Seq analyses of archival sera specimens

    doi: 10.1371/journal.pone.0196913

    Figure Lengend Snippet: Comparison of sera exosomal RNA using four different RNA extraction methods. (A) Total RNA yield from ultracentrifugation (UC) and ExoQuick (EQ) treated samples using the RNeasy Mini Kit combined with TRIzol LS, the RNeasy Mini Kit alone, conventional RNA precipitation, and AllPrep DNA/RNA Mini Kit. (B) Demonstration of RNA quality measured by OD 260 /OD 280 in EQ and UC treated samples. Data are shown as the mean ± SD from six independent patient samples. *** P

    Article Snippet: Exosomal RNA isolation via AllPrep DNA/RNA Mini Kit The AllPrep DNA/RNA Mini kit (Qiagen, catalogue number: 80204) was used to extract exosomal RNAs following the manufacturer’s protocol.

    Techniques: RNA Extraction

    IgA ablation inhibits, while CD8 deficiency accelerates HCC development a , b , Total DNA was extracted from HCC nodules of 11-month-old mice ( n = 16) of the indicated genotypes with or without anti-PD-L1 treatment, and subjected to exome sequencing. Shown are the number of point mutations identified per sample ( a ) and mutational signatures ( b ). Horizontal axis shows the 96-substitution patterns with substitution subtypes on top, and vertical axis indicates the probability of each pattern in b. c , Top 20 hallmark gene sets sorted by normalized enrichment score (NES) are shown to depict HCC progression in MUP-uPA and MUP-uPA/Iga −/− for human HCC are marked with a red asterisk. d , Total RNA was extracted from livers of 6-month-old mice and from HCC nodules of 11-month-old mice of the indicated genotypes and subjected to RNA-seq analysis. Hierarchical clustering of gene expression profiles comparing HCC and non-HCC mouse samples according to the RNA-seq. The top three enriched pathways/functional categories from Metascape are reported for major clusters of genes. e , Representative images of liver histology at different time points and indicated strains are shown with detailed n values. Scale bars, 100 μm (3 months), 250 μm (6, 11 months). f , Total tumour numbers in 3-month-old MUP-uPA mice ( n = 11, 7, 8). g , Heat map depicting differential expression of 17 liver-specific genes and 33 HCC-related genes in the indicated strains, illustrating the upregulation of some HCC-related genes in MUP-uPA/Cd8a −/− livers at 6 months of age (total mice number 29; n = 3 or 4 per group). h – j , BL6 mice of the indicated phenotype were subjected to the STAM protocol and their tumour volumes ( n = 14, 6, 6, 4, 3, 9, 3) ( h ) and histopathology ( i , j ) were evaluated at 25 weeks of age. The data were validated at least in two or three experiments. Paraffin-embedded and frozen liver sections from these mice were stained with haematoxylin and eosin, Sirius Red, or Oil Red O, as indicated. Shown are typical images of tumour-containing and tumour-free areas, the borders between which are marked by the black lines. Scale bars: haematoxylin and eosin, 100 μm; Sirius Red, 100, 250, or 500 μm; Oil Red O, 50 μm. Oil Red O-positive areas were quantitated and are shown on the right. The Sirius Red-stained areas for each mouse were calculated by image analysis of the whole-tissue scan and normalized to the haematoxylin and eosin stain ( n = 3 or 4 per group). k , Tumour volumes are shown for STAM-WT ( n = 10), STAM- Iga −/− ( n = 13), and STAM- Iga −/− after CD8 depletion ( n = 3). l , MUP-uPA/Iga −/− -HFD mice were injected weekly with anti-CD8 for 6 weeks and tumour multiplicity was determined ( n = 3). k , l , CD8 depletion experiments were repeated using two different HCC models (MUP and STAM). m , Heat map depicting the differential expression of 59 genes involved in allograft rejection, IFNγ response, and inflammation (total mouse number 41; 6 months: n = 3 and 11 months: 3 or 4 per group). n , o , Paraffin-embedded and frozen liver sections from 11-month-old mice ( n ) and adoptively transferred mice ( o ) were stained with haematoxylin and eosin, Sirius Red, or Oil Red O as indicated and analysed (Sirius Red: n = 9, 16, 14, 6; Oil Red O: n = 8, 5, 5, 8 for n ). Shown are typical images of tumourcontaining and tumour-free areas, the borders between which are marked by the black lines. p , Liver cells from MUP-uPA/Rag1 −/− mice 1 week after being adoptively transferred with CFSE-labelled T cells with or without B cells as indicated ( n = 3 in each group) were stained and analysed by flow cytometry. Shown are the percentage of CD8 + T cells among CD45 + cells (left), and histogram of proliferating CFSE-labelled T cells with the corresponding mean fluorescence intensity (right). q , Liver sections for MUP-uPA/Rag1 −/− and the corresponding adoptive lymphocyte transfer mice (4 weeks after adoptive transfer (AT)) were stained with alpha-SMA, IgA, CD3, and CD8 antibodies, counterstained with DAPI and examined by fluorescent microscopy (scale bars, 50 μm). For CD3/CD8 staining, images with higher magnification are shown (scale bars, 20 μm). Single-cell suspensions were prepared from the corresponding liver, stained with antibodies to CD45, IgA, CD19, B220, CD8, and CD4, and analysed by flow cytometry. Shown are representative scatter plots. MUP;Rag1 −/− mouse livers have been used for validation of CD4, CD8, IgA, and CD19, both for flow cytometry and for immunofluorescence analyses. The data were validated in at least two experiments. r , STAM-BL6 mice of the indicated phenotypes were analysed for IgA serum amounts by ELISA ( n = 11, 4, 4, 4, 4). s , Absolute IgA + cell number in livers of indicated STAM-BL6 mice ( n = 13, 4, 4, 9, 8). Each dot represents a mouse. Two-sided t -test (means ± s.e.m.; f , j , l , n , r ) and Mann–Whitney test (median; h , k , n , s ) were used to determine significance. * P

    Journal: Nature

    Article Title: Inflammation-induced IgA+ cells dismantle anti-liver cancer immunity

    doi: 10.1038/nature24302

    Figure Lengend Snippet: IgA ablation inhibits, while CD8 deficiency accelerates HCC development a , b , Total DNA was extracted from HCC nodules of 11-month-old mice ( n = 16) of the indicated genotypes with or without anti-PD-L1 treatment, and subjected to exome sequencing. Shown are the number of point mutations identified per sample ( a ) and mutational signatures ( b ). Horizontal axis shows the 96-substitution patterns with substitution subtypes on top, and vertical axis indicates the probability of each pattern in b. c , Top 20 hallmark gene sets sorted by normalized enrichment score (NES) are shown to depict HCC progression in MUP-uPA and MUP-uPA/Iga −/− for human HCC are marked with a red asterisk. d , Total RNA was extracted from livers of 6-month-old mice and from HCC nodules of 11-month-old mice of the indicated genotypes and subjected to RNA-seq analysis. Hierarchical clustering of gene expression profiles comparing HCC and non-HCC mouse samples according to the RNA-seq. The top three enriched pathways/functional categories from Metascape are reported for major clusters of genes. e , Representative images of liver histology at different time points and indicated strains are shown with detailed n values. Scale bars, 100 μm (3 months), 250 μm (6, 11 months). f , Total tumour numbers in 3-month-old MUP-uPA mice ( n = 11, 7, 8). g , Heat map depicting differential expression of 17 liver-specific genes and 33 HCC-related genes in the indicated strains, illustrating the upregulation of some HCC-related genes in MUP-uPA/Cd8a −/− livers at 6 months of age (total mice number 29; n = 3 or 4 per group). h – j , BL6 mice of the indicated phenotype were subjected to the STAM protocol and their tumour volumes ( n = 14, 6, 6, 4, 3, 9, 3) ( h ) and histopathology ( i , j ) were evaluated at 25 weeks of age. The data were validated at least in two or three experiments. Paraffin-embedded and frozen liver sections from these mice were stained with haematoxylin and eosin, Sirius Red, or Oil Red O, as indicated. Shown are typical images of tumour-containing and tumour-free areas, the borders between which are marked by the black lines. Scale bars: haematoxylin and eosin, 100 μm; Sirius Red, 100, 250, or 500 μm; Oil Red O, 50 μm. Oil Red O-positive areas were quantitated and are shown on the right. The Sirius Red-stained areas for each mouse were calculated by image analysis of the whole-tissue scan and normalized to the haematoxylin and eosin stain ( n = 3 or 4 per group). k , Tumour volumes are shown for STAM-WT ( n = 10), STAM- Iga −/− ( n = 13), and STAM- Iga −/− after CD8 depletion ( n = 3). l , MUP-uPA/Iga −/− -HFD mice were injected weekly with anti-CD8 for 6 weeks and tumour multiplicity was determined ( n = 3). k , l , CD8 depletion experiments were repeated using two different HCC models (MUP and STAM). m , Heat map depicting the differential expression of 59 genes involved in allograft rejection, IFNγ response, and inflammation (total mouse number 41; 6 months: n = 3 and 11 months: 3 or 4 per group). n , o , Paraffin-embedded and frozen liver sections from 11-month-old mice ( n ) and adoptively transferred mice ( o ) were stained with haematoxylin and eosin, Sirius Red, or Oil Red O as indicated and analysed (Sirius Red: n = 9, 16, 14, 6; Oil Red O: n = 8, 5, 5, 8 for n ). Shown are typical images of tumourcontaining and tumour-free areas, the borders between which are marked by the black lines. p , Liver cells from MUP-uPA/Rag1 −/− mice 1 week after being adoptively transferred with CFSE-labelled T cells with or without B cells as indicated ( n = 3 in each group) were stained and analysed by flow cytometry. Shown are the percentage of CD8 + T cells among CD45 + cells (left), and histogram of proliferating CFSE-labelled T cells with the corresponding mean fluorescence intensity (right). q , Liver sections for MUP-uPA/Rag1 −/− and the corresponding adoptive lymphocyte transfer mice (4 weeks after adoptive transfer (AT)) were stained with alpha-SMA, IgA, CD3, and CD8 antibodies, counterstained with DAPI and examined by fluorescent microscopy (scale bars, 50 μm). For CD3/CD8 staining, images with higher magnification are shown (scale bars, 20 μm). Single-cell suspensions were prepared from the corresponding liver, stained with antibodies to CD45, IgA, CD19, B220, CD8, and CD4, and analysed by flow cytometry. Shown are representative scatter plots. MUP;Rag1 −/− mouse livers have been used for validation of CD4, CD8, IgA, and CD19, both for flow cytometry and for immunofluorescence analyses. The data were validated in at least two experiments. r , STAM-BL6 mice of the indicated phenotypes were analysed for IgA serum amounts by ELISA ( n = 11, 4, 4, 4, 4). s , Absolute IgA + cell number in livers of indicated STAM-BL6 mice ( n = 13, 4, 4, 9, 8). Each dot represents a mouse. Two-sided t -test (means ± s.e.m.; f , j , l , n , r ) and Mann–Whitney test (median; h , k , n , s ) were used to determine significance. * P

    Article Snippet: Total RNA and DNA was extracted using an AllPrep DNA/RNA Mini Kit (Qiagen).

    Techniques: Mouse Assay, Sequencing, RNA Sequencing Assay, Expressing, Functional Assay, Histopathology, Staining, H&E Stain, Injection, Flow Cytometry, Cytometry, Fluorescence, Adoptive Transfer Assay, Microscopy, Immunofluorescence, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY