Structured Review

Promega 800uml 1 rnasin
800uml 1 Rnasin, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/800uml 1 rnasin/product/Promega
Average 78 stars, based on 1 article reviews
Price from $9.99 to $1999.99
800uml 1 rnasin - by Bioz Stars, 2020-03
78/100 stars

Related Products / Commonly Used Together

dntps
50ul reaction
oligo-dt
rna
96oc
42oc
dtt

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Related Articles

Polymerase Chain Reaction:

Article Title: Recruitment of a distinct but related set of VH sequences into the murine CD21hi/CD23- marginal zone B-cell repertoire to that seen in the class switched antibody response
Article Snippet: 20% of the RNA obtained from a whole cell-sort sample was denatured at 96oC for 3min with 0.5ug oligo-dT and placed on ice before reverse transcription in a 50ul reaction for 1hr at 42oC with 1x RT buffer, 0.2mM dNTPs, 1mM DTT, 800Uml-1 RNAsin (Promega) and 200U Superscript II reverse transcriptase. .. First round PCR was done with 2ul of cDNA reaction with standard reaction conditions using manufacturers supplied buffer with Hotstart Pfu turbo polymerase (Agilent Stratagene, UK) and 25pmol of the appropriate primer in a 50ul reaction for 34 cycles with the following cycling: 96oC, 30s; 59oC, 30s; 72oC, 2min; preceded by an incubation at 96oC for 3min.

RNA Extraction:

Article Title: Recruitment of a distinct but related set of VH sequences into the murine CD21hi/CD23- marginal zone B-cell repertoire to that seen in the class switched antibody response
Article Snippet: Briefly: Total RNA extraction was performed using the RNA Bee reagent (amsbio, U.K.) according to the manufacturers instructions. .. 20% of the RNA obtained from a whole cell-sort sample was denatured at 96oC for 3min with 0.5ug oligo-dT and placed on ice before reverse transcription in a 50ul reaction for 1hr at 42oC with 1x RT buffer, 0.2mM dNTPs, 1mM DTT, 800Uml-1 RNAsin (Promega) and 200U Superscript II reverse transcriptase.

Incubation:

Article Title: Recruitment of a distinct but related set of VH sequences into the murine CD21hi/CD23- marginal zone B-cell repertoire to that seen in the class switched antibody response
Article Snippet: 20% of the RNA obtained from a whole cell-sort sample was denatured at 96oC for 3min with 0.5ug oligo-dT and placed on ice before reverse transcription in a 50ul reaction for 1hr at 42oC with 1x RT buffer, 0.2mM dNTPs, 1mM DTT, 800Uml-1 RNAsin (Promega) and 200U Superscript II reverse transcriptase. .. First round PCR was done with 2ul of cDNA reaction with standard reaction conditions using manufacturers supplied buffer with Hotstart Pfu turbo polymerase (Agilent Stratagene, UK) and 25pmol of the appropriate primer in a 50ul reaction for 34 cycles with the following cycling: 96oC, 30s; 59oC, 30s; 72oC, 2min; preceded by an incubation at 96oC for 3min.

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    Promega 800uml 1 rnasin
    800uml 1 Rnasin, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/800uml 1 rnasin/product/Promega
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    800uml 1 rnasin - by Bioz Stars, 2020-03
    78/100 stars
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