normal tissue types  (AMS Biotechnology)

Journal: mAbs

Article Title: Novel human monoclonal antibodies specific to the alternatively spliced domain D of Tenascin C efficiently target tumors in vivo

doi: 10.1080/19420862.2020.1836713

Figure Lengend Snippet: Therapy in BALB/c nude mice bearing SKRC52 human renal cell carcinoma and in VM/Dk mice bearing SMA-497 glioma

Article Snippet: Antigen expression was confirmed on ice-cold acetone-fixed 10-µm cryostat sections of A431, U87, SKRC52, A375, Colon 26, C51, SMA-540, SMA-497, of frozen tumor and normal tissue specimens in microarray (Amsbio, T2635700) and of patient-derived glioblastoma stained with R6N IgG1-FITC (protein was FITC labeled according to manufacturer protocol; Sigma) (final concentration 10 µg/mL) and detected with rabbit anti-FITC (Bio-Rad, 4510–7804) and anti-rabbit AlexaFluor488 (Invitrogen; A11008).

Techniques:

Journal: Molecular cancer therapeutics

Article Title: Potency-matched dual cytokine-antibody fusion proteins for cancer therapy

doi: 10.1158/1535-7163.MCT-17-0211

Figure Lengend Snippet: A microarray containing normal tissue specimens (left) and their tumoral counterpart (right) was stained with a biotinylated preparation of the fully human IL2-F8-TNFmut (green, Alexa Fluor 488), cell nuclei were counterstained with DAPI (blue), 20x magnification, scale bars = 100µm.

Article Snippet: Frozen tumor and normal tissue specimens in microarray format were obtained from Amsbio and stained with a biotinylated preparation of the fully human IL2-F8-TNF mut fusion protein and detected with Streptavidin-AlexaFluor488 (Invitrogen {"type":"entrez-protein","attrs":{"text":"S11223","term_id":"112468","term_text":"pir||S11223"}} S11223 ).

Techniques: Microarray, Staining