Structured Review

Difco 7h9 liquid growth medium
7h9 Liquid Growth Medium, supplied by Difco, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/7h9 liquid growth medium/product/Difco
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
7h9 liquid growth medium - by Bioz Stars, 2020-04
93/100 stars

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Related Articles

In Vitro:

Article Title: Susceptibility of Mycobacterium tuberculosis Cytochrome bd Oxidase Mutants to Compounds Targeting the Terminal Respiratory Oxidase, Cytochrome c
Article Snippet: In a key study published during the preparation of the manuscript, Berney, Pethe, and colleagues ( ) reported that targeted disruption of cydAB eliminated oxygen respiration in M. tuberculosis bacilli exposed to Q203, killing the cells and rendering the resulting Δ cydAB mutant strain hypersusceptible to Q203 treatment in vitro in both replicating and nonreplicating (tolerant) conditions as well as in a mouse model. .. In standard microplate-based alamarBlue assays (MABA) ( ) using Middlebrook 7H9 liquid growth medium (Difco) supplemented with 10% oleic acid-albumin-dextrose-catalase (OADC), 0.5% glycerol, and 0.05% Tween 80, the MIC90 values recorded for a representative panel of approved anti-TB agents from different antibiotic classes and with diverse mechanisms of action were the same for cydA and cydAB mutants and identical to those observed for wild-type M. tuberculosis H37Rv and the cydKO ( cydC :: aph ) mutant strain ( ).

Mutagenesis:

Article Title: Susceptibility of Mycobacterium tuberculosis Cytochrome bd Oxidase Mutants to Compounds Targeting the Terminal Respiratory Oxidase, Cytochrome c
Article Snippet: .. In standard microplate-based alamarBlue assays (MABA) ( ) using Middlebrook 7H9 liquid growth medium (Difco) supplemented with 10% oleic acid-albumin-dextrose-catalase (OADC), 0.5% glycerol, and 0.05% Tween 80, the MIC90 values recorded for a representative panel of approved anti-TB agents from different antibiotic classes and with diverse mechanisms of action were the same for cydA and cydAB mutants and identical to those observed for wild-type M. tuberculosis H37Rv and the cydKO ( cydC :: aph ) mutant strain ( ). ..

Construct:

Article Title: Susceptibility of Mycobacterium tuberculosis Cytochrome bd Oxidase Mutants to Compounds Targeting the Terminal Respiratory Oxidase, Cytochrome c
Article Snippet: In that case, Δ cydAB mutants were constructed using a phage-mediated unmarking ( ) system that leaves an approximately 130-bp “scar” at the deletion site following activity of the γδ resolvase. .. In standard microplate-based alamarBlue assays (MABA) ( ) using Middlebrook 7H9 liquid growth medium (Difco) supplemented with 10% oleic acid-albumin-dextrose-catalase (OADC), 0.5% glycerol, and 0.05% Tween 80, the MIC90 values recorded for a representative panel of approved anti-TB agents from different antibiotic classes and with diverse mechanisms of action were the same for cydA and cydAB mutants and identical to those observed for wild-type M. tuberculosis H37Rv and the cydKO ( cydC :: aph ) mutant strain ( ).

Generated:

Article Title: Susceptibility of Mycobacterium tuberculosis Cytochrome bd Oxidase Mutants to Compounds Targeting the Terminal Respiratory Oxidase, Cytochrome c
Article Snippet: Here, we generated targeted, in-frame deletion mutants of cydA and cydAB in M. tuberculosis H37RvMA ( ) using two-step allelic exchange mutagenesis ( ) in order to preserve the sequence integrity of the locus. .. In standard microplate-based alamarBlue assays (MABA) ( ) using Middlebrook 7H9 liquid growth medium (Difco) supplemented with 10% oleic acid-albumin-dextrose-catalase (OADC), 0.5% glycerol, and 0.05% Tween 80, the MIC90 values recorded for a representative panel of approved anti-TB agents from different antibiotic classes and with diverse mechanisms of action were the same for cydA and cydAB mutants and identical to those observed for wild-type M. tuberculosis H37Rv and the cydKO ( cydC :: aph ) mutant strain ( ).

Activity Assay:

Article Title: Susceptibility of Mycobacterium tuberculosis Cytochrome bd Oxidase Mutants to Compounds Targeting the Terminal Respiratory Oxidase, Cytochrome c
Article Snippet: In that case, Δ cydAB mutants were constructed using a phage-mediated unmarking ( ) system that leaves an approximately 130-bp “scar” at the deletion site following activity of the γδ resolvase. .. In standard microplate-based alamarBlue assays (MABA) ( ) using Middlebrook 7H9 liquid growth medium (Difco) supplemented with 10% oleic acid-albumin-dextrose-catalase (OADC), 0.5% glycerol, and 0.05% Tween 80, the MIC90 values recorded for a representative panel of approved anti-TB agents from different antibiotic classes and with diverse mechanisms of action were the same for cydA and cydAB mutants and identical to those observed for wild-type M. tuberculosis H37Rv and the cydKO ( cydC :: aph ) mutant strain ( ).

Inhibition:

Article Title: Susceptibility of Mycobacterium tuberculosis Cytochrome bd Oxidase Mutants to Compounds Targeting the Terminal Respiratory Oxidase, Cytochrome c
Article Snippet: It was not clear, therefore, when we initiated the current study whether disruption of the entire locus was phenotypically equivalent to targeted deletion (or, by implication, chemical inhibition) of the individual genes; moreover, no reports at the time had attempted to unlink the effects of disrupted cytochrome bd menaquinol oxidase function ( cydAB inactivation) from deficient ABC transport ( cydDC inactivation). .. In standard microplate-based alamarBlue assays (MABA) ( ) using Middlebrook 7H9 liquid growth medium (Difco) supplemented with 10% oleic acid-albumin-dextrose-catalase (OADC), 0.5% glycerol, and 0.05% Tween 80, the MIC90 values recorded for a representative panel of approved anti-TB agents from different antibiotic classes and with diverse mechanisms of action were the same for cydA and cydAB mutants and identical to those observed for wild-type M. tuberculosis H37Rv and the cydKO ( cydC :: aph ) mutant strain ( ).

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    Difco 7h9 liquid growth medium
    7h9 Liquid Growth Medium, supplied by Difco, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7h9 liquid growth medium/product/Difco
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    7h9 liquid growth medium - by Bioz Stars, 2020-04
    93/100 stars
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