qiashredder  (Qiagen)


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    Name:
    QIAshredder
    Description:
    For simple and rapid homogenization of cell and tissue lysates Kit contents Qiagen QIAshredder 50 Disposable Cell lysate Homogenizers for use in Nucleic acid preps Caps For Simple and Rapid Homogenization of Cell and Tissue Lysates Replaces Syringe and needle Homogenization Reduces Loss of Sample Material Eliminates Cross contamination Between Samples Filters out Insoluble Debris and Reduces Viscosity Spin column Format QIAshredder Homogenizer Placed in a Collection Tube and Centrifuged Benefits Replaces syringe and needle homogenization Reduces loss of sample material Eliminates cross contamination between samples Filters out insoluble debris and reduces viscosity
    Catalog Number:
    79654
    Price:
    88
    Category:
    QIAshredder
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    Structured Review

    Qiagen qiashredder
    QIAshredder
    For simple and rapid homogenization of cell and tissue lysates Kit contents Qiagen QIAshredder 50 Disposable Cell lysate Homogenizers for use in Nucleic acid preps Caps For Simple and Rapid Homogenization of Cell and Tissue Lysates Replaces Syringe and needle Homogenization Reduces Loss of Sample Material Eliminates Cross contamination Between Samples Filters out Insoluble Debris and Reduces Viscosity Spin column Format QIAshredder Homogenizer Placed in a Collection Tube and Centrifuged Benefits Replaces syringe and needle homogenization Reduces loss of sample material Eliminates cross contamination between samples Filters out insoluble debris and reduces viscosity
    https://www.bioz.com/result/qiashredder/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    qiashredder - by Bioz Stars, 2021-06
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Hydroponic Treatment of Nicotiana benthamiana with Kifunensine Modifies the N-glycans of Recombinant Glycoprotein Antigens to Predominantly Man9 High-Mannose Type upon Transient Overexpression
    Article Snippet: .. The samples were prepared by QIAShredder (Qiagen) and RNAqueous Phenol-free total RNA Isolation Kits (Thermo Fisher Scientific, Waltham, MA), following manufacturer's protocol. .. After total RNA isolation, TURBO DNA free kit (Thermo Fisher Scientific) was used to eliminate genomic DNA.

    Article Title: Cross-species transcriptional analysis reveals conserved and host-specific neoplastic processes in mammalian glioma
    Article Snippet: .. RNA was isolated from tumor cells using the RNAeasy Mini Kit and QIAshredder (QIAGEN, Valencia, CA, USA), according to the manufacturer’s protocol. .. The RNA concentration was determined using a NanoDrop ND-1000 UV-Vis spectrophotometer (NanoDrop Technologies, Wilmington, DE) and quality was measured using a 2100 Bioanalyzer (Agilent, Santa Clara, CA, USA) with the 6000 chip. ) was used to generate, amplify, and label biotinylated cRNA.

    Homogenization:

    Article Title: Potential of Tyrosine Kinase Receptor TIE-1 as Novel Therapeutic Target in High-PI3K-Expressing Ovarian Cancer
    Article Snippet: Cell viability was determined using a CellTiter-Glo luminescent cell-viability assay (Promega) according to the manufacturer’s protocol. .. Quantitative Real-Time PCRTotal RNA was extracted after the homogenization of cell samples using the QIAshredder and RNeasy mini kits (Qiagen, Hilden, Germany). .. Extracted RNAs were reverse-transcribed with SuperScript III (Thermo Fisher Scientific) for cDNA synthesis.

    Purification:

    Article Title: Enhanced ZnR/GPR39 Activity in Breast Cancer, an Alternative Trigger of Signaling Leading to Cell Growth
    Article Snippet: To determine GPR39 mRNA level, cells were seeded on 60-mm plates and scraped after 48 h using RNeasy Mini Kit lysis buffer (QIAGEN, Germany). .. Cell lysates were homogenized using QIAshredder and total RNA was purified using RNeasy Mini Kit as described by the manufacturer (QIAGEN, Germany). .. 1 μg RNA was converted to cDNA using qScript cDNA synthesis kit (Quanta Biosciences, MA, USA).

    Article Title: Discovery of a Novel, Monocationic, Small-Molecule Inhibitor of Scrapie Prion Accumulation in Cultured Sheep Microglia and Rov Cells
    Article Snippet: For RT-PCR, the washed cell pellet was lysed in buffer QLT (Qiagen, Valencia, CA, USA). .. Cell lysates were shredded (QiaShredder, Qiagen) and total RNA was purified using RNeasy mini spin columns (Qiagen) following manufacturer's directions. .. Total RNA quantity and quality was determined by spectrophotometry (Thermo Scientific, ND-1000).

    Article Title: Immunogene therapy with fusogenic nanoparticles modulates macrophage response to Staphylococcus aureus
    Article Snippet: The in vivo knockdown of Irf5 was quantified using two-step qRT-PCR (Roche LightCycler 96). .. The cell pellets or the lung homogenates were lysed and RNA was purified using the QIAshredder and RNeasy Mini Kit (Qiagen). cDNA was transcribed from the purified RNA using the BIORAD iScript cDNA Synthesis Kit and heat-treated in the Eppendorf Vapo.protect Mastercycler thermal cycler. cDNA was mixed with Irf5 primers, or the control HPRT primers ( Irf5 forward: 5′-AATACCCCACCACCTTTTGA-3′; Irf5 reverse: 5′-TTGAGATCCGGGTTTGAGAT-3′; HPRT forward: 5′-GTCAACGGGGGACATAAAAG-3′; HPRT reverse: 5′-CAACAATCAAGACATT-CTTTCCA-3′) and iQ SYBR Green Supermix according to the manufacturer’s instructions. qRT-PCR analysis was performed in the BIORAD 96-well white Multiplate PCR Plates using the Roche LightCycler 96. .. The quantification was performed at n = 6 and in RNAse- and DNAse-free laminar flow hood dedicated to RNA work.

    RNA Extraction:

    Article Title: The drought-tolerant Solanum pennellii regulates leaf water loss and induces genes involved in amino acid and ethylene/jasmonate metabolism under dehydration
    Article Snippet: RNA isolated from leaflets coming from three individual pooled plants (biological replicates) was used in hybridization to one chip, resulting in total 12 chips (three replicates per each species and treatment). .. RNA extraction was performed with RNeasy Mini kit and QIAshredder (Qiagen) following the manufacturer’s instructions, and the amount and quality of the RNA checked by Bioanalyzer and spectrophotometrically by Nanodrop. .. Biotinylated cRNA was synthetized from 200 ng of each sample using the GeneChip 3′ IVT Express kit (Affymetrix), according to the protocol supplied by the manufacturer.

    SYBR Green Assay:

    Article Title: Immunogene therapy with fusogenic nanoparticles modulates macrophage response to Staphylococcus aureus
    Article Snippet: The in vivo knockdown of Irf5 was quantified using two-step qRT-PCR (Roche LightCycler 96). .. The cell pellets or the lung homogenates were lysed and RNA was purified using the QIAshredder and RNeasy Mini Kit (Qiagen). cDNA was transcribed from the purified RNA using the BIORAD iScript cDNA Synthesis Kit and heat-treated in the Eppendorf Vapo.protect Mastercycler thermal cycler. cDNA was mixed with Irf5 primers, or the control HPRT primers ( Irf5 forward: 5′-AATACCCCACCACCTTTTGA-3′; Irf5 reverse: 5′-TTGAGATCCGGGTTTGAGAT-3′; HPRT forward: 5′-GTCAACGGGGGACATAAAAG-3′; HPRT reverse: 5′-CAACAATCAAGACATT-CTTTCCA-3′) and iQ SYBR Green Supermix according to the manufacturer’s instructions. qRT-PCR analysis was performed in the BIORAD 96-well white Multiplate PCR Plates using the Roche LightCycler 96. .. The quantification was performed at n = 6 and in RNAse- and DNAse-free laminar flow hood dedicated to RNA work.

    Quantitative RT-PCR:

    Article Title: Immunogene therapy with fusogenic nanoparticles modulates macrophage response to Staphylococcus aureus
    Article Snippet: The in vivo knockdown of Irf5 was quantified using two-step qRT-PCR (Roche LightCycler 96). .. The cell pellets or the lung homogenates were lysed and RNA was purified using the QIAshredder and RNeasy Mini Kit (Qiagen). cDNA was transcribed from the purified RNA using the BIORAD iScript cDNA Synthesis Kit and heat-treated in the Eppendorf Vapo.protect Mastercycler thermal cycler. cDNA was mixed with Irf5 primers, or the control HPRT primers ( Irf5 forward: 5′-AATACCCCACCACCTTTTGA-3′; Irf5 reverse: 5′-TTGAGATCCGGGTTTGAGAT-3′; HPRT forward: 5′-GTCAACGGGGGACATAAAAG-3′; HPRT reverse: 5′-CAACAATCAAGACATT-CTTTCCA-3′) and iQ SYBR Green Supermix according to the manufacturer’s instructions. qRT-PCR analysis was performed in the BIORAD 96-well white Multiplate PCR Plates using the Roche LightCycler 96. .. The quantification was performed at n = 6 and in RNAse- and DNAse-free laminar flow hood dedicated to RNA work.

    Polymerase Chain Reaction:

    Article Title: Immunogene therapy with fusogenic nanoparticles modulates macrophage response to Staphylococcus aureus
    Article Snippet: The in vivo knockdown of Irf5 was quantified using two-step qRT-PCR (Roche LightCycler 96). .. The cell pellets or the lung homogenates were lysed and RNA was purified using the QIAshredder and RNeasy Mini Kit (Qiagen). cDNA was transcribed from the purified RNA using the BIORAD iScript cDNA Synthesis Kit and heat-treated in the Eppendorf Vapo.protect Mastercycler thermal cycler. cDNA was mixed with Irf5 primers, or the control HPRT primers ( Irf5 forward: 5′-AATACCCCACCACCTTTTGA-3′; Irf5 reverse: 5′-TTGAGATCCGGGTTTGAGAT-3′; HPRT forward: 5′-GTCAACGGGGGACATAAAAG-3′; HPRT reverse: 5′-CAACAATCAAGACATT-CTTTCCA-3′) and iQ SYBR Green Supermix according to the manufacturer’s instructions. qRT-PCR analysis was performed in the BIORAD 96-well white Multiplate PCR Plates using the Roche LightCycler 96. .. The quantification was performed at n = 6 and in RNAse- and DNAse-free laminar flow hood dedicated to RNA work.

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  • 99
    Qiagen qiashredder
    Qiashredder, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiashredder/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    qiashredder - by Bioz Stars, 2021-06
    99/100 stars
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