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human notch1 pathway reporter kit  (AMS Biotechnology)


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    AMS Biotechnology human notch1 pathway reporter kit
    Human Notch1 Pathway Reporter Kit, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/79503/pm40240593-674-19-24?v=AMS+Biotechnology
    Average 93 stars, based on 11 article reviews
    human notch1 pathway reporter kit - by Bioz Stars, 2026-07
    93/100 stars

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    human notch1 pathway reporter kit  (AMS Biotechnology)
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    AMS Biotechnology human notch1 pathway reporter kit
    Human Notch1 Pathway Reporter Kit, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/79503/pm40240593-674-19-24?v=AMS+Biotechnology
    Average 93 stars, based on 1 article reviews
    human notch1 pathway reporter kit - by Bioz Stars, 2026-07
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    A Boyden chamber transwell migration and invasion assays in H1299 cells transfected with EGFP-ACBD3 or EGFP-ACBD3 lacking the NUMB-binding domain (ΔNUMB). B Correlation between ACBD3, NUMB, NOTCHs, or NOTCH target genes mRNA levels and EMT scores. C Schema: The NOTCH luciferase reporter. CBF1/RBPJκ/Suppressor of Hairless/Lag-1 (CSL) response element (RE) is fused upstream of the firefly luciferase gene. NICD Notch intracellular domain, Graph luciferase reporter assay in H441 cells co-transfected with luciferase reporter and shACBD3 or <t>NOTCH1</t> NICD. n = 4. D WB analysis of ACBD3 and EGFP-ACBD3 proteins in H1299 cells transfectants. E NOTCH luciferase reporter assay. F , G , I qPCR analysis of mRNA levels of ACBD3 and NOTCH target genes in shACBD3-tranfected H441 ( F ) and A549 ( G ), and ACBD3-transfected H1299 ( I ) cells. n = 4. H , J , K WB analysis of SNAI1 and ACBD3 protein levels in lung cancer cells transfected with shACBD3 ( H ), ACBD3 expression vector ( J ), or shACBD3 and SNAI1 siRNA ( K ). L , M Transwell migration ( L ) and apoptosis ( M ) assays of cell transfectants in ( K ). N Working model: ACDB3 inhibits the NOTCH-SNAI1 axis to suppress cancer cell motility and metastasis. Data indicate the mean ± SD from a single experiment incorporating biological replicate samples ( n = 3, unless otherwise indicated) and are representative of at least 2 independent experiments. P -values were determined using two-tailed Student’s t-test (for F , G , I ), one-way ANOVA test (for A , E , L , M ).
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    notch1 pathway  (BPS Bioscience)
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    A Boyden chamber transwell migration and invasion assays in H1299 cells transfected with EGFP-ACBD3 or EGFP-ACBD3 lacking the NUMB-binding domain (ΔNUMB). B Correlation between ACBD3, NUMB, NOTCHs, or NOTCH target genes mRNA levels and EMT scores. C Schema: The NOTCH luciferase reporter. CBF1/RBPJκ/Suppressor of Hairless/Lag-1 (CSL) response element (RE) is fused upstream of the firefly luciferase gene. NICD Notch intracellular domain, Graph luciferase reporter assay in H441 cells co-transfected with luciferase reporter and shACBD3 or <t>NOTCH1</t> NICD. n = 4. D WB analysis of ACBD3 and EGFP-ACBD3 proteins in H1299 cells transfectants. E NOTCH luciferase reporter assay. F , G , I qPCR analysis of mRNA levels of ACBD3 and NOTCH target genes in shACBD3-tranfected H441 ( F ) and A549 ( G ), and ACBD3-transfected H1299 ( I ) cells. n = 4. H , J , K WB analysis of SNAI1 and ACBD3 protein levels in lung cancer cells transfected with shACBD3 ( H ), ACBD3 expression vector ( J ), or shACBD3 and SNAI1 siRNA ( K ). L , M Transwell migration ( L ) and apoptosis ( M ) assays of cell transfectants in ( K ). N Working model: ACDB3 inhibits the NOTCH-SNAI1 axis to suppress cancer cell motility and metastasis. Data indicate the mean ± SD from a single experiment incorporating biological replicate samples ( n = 3, unless otherwise indicated) and are representative of at least 2 independent experiments. P -values were determined using two-tailed Student’s t-test (for F , G , I ), one-way ANOVA test (for A , E , L , M ).
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    notch1 pathway reporter kit  (AMS Biotechnology)
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    AMS Biotechnology notch1 pathway reporter kit
    A Boyden chamber transwell migration and invasion assays in H1299 cells transfected with EGFP-ACBD3 or EGFP-ACBD3 lacking the NUMB-binding domain (ΔNUMB). B Correlation between ACBD3, NUMB, NOTCHs, or NOTCH target genes mRNA levels and EMT scores. C Schema: The NOTCH luciferase reporter. CBF1/RBPJκ/Suppressor of Hairless/Lag-1 (CSL) response element (RE) is fused upstream of the firefly luciferase gene. NICD Notch intracellular domain, Graph luciferase reporter assay in H441 cells co-transfected with luciferase reporter and shACBD3 or <t>NOTCH1</t> NICD. n = 4. D WB analysis of ACBD3 and EGFP-ACBD3 proteins in H1299 cells transfectants. E NOTCH luciferase reporter assay. F , G , I qPCR analysis of mRNA levels of ACBD3 and NOTCH target genes in shACBD3-tranfected H441 ( F ) and A549 ( G ), and ACBD3-transfected H1299 ( I ) cells. n = 4. H , J , K WB analysis of SNAI1 and ACBD3 protein levels in lung cancer cells transfected with shACBD3 ( H ), ACBD3 expression vector ( J ), or shACBD3 and SNAI1 siRNA ( K ). L , M Transwell migration ( L ) and apoptosis ( M ) assays of cell transfectants in ( K ). N Working model: ACDB3 inhibits the NOTCH-SNAI1 axis to suppress cancer cell motility and metastasis. Data indicate the mean ± SD from a single experiment incorporating biological replicate samples ( n = 3, unless otherwise indicated) and are representative of at least 2 independent experiments. P -values were determined using two-tailed Student’s t-test (for F , G , I ), one-way ANOVA test (for A , E , L , M ).
    Notch1 Pathway Reporter Kit, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/79503/custom%4079503%4010%2E1101%2F2024%2E08%2E30%2E610426?v=AMS+Biotechnology
    Average 93 stars, based on 1 article reviews
    notch1 pathway reporter kit - by Bioz Stars, 2026-07
    93/100 stars
    		  Buy from Supplier

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    A Boyden chamber transwell migration and invasion assays in H1299 cells transfected with EGFP-ACBD3 or EGFP-ACBD3 lacking the NUMB-binding domain (ΔNUMB). B Correlation between ACBD3, NUMB, NOTCHs, or NOTCH target genes mRNA levels and EMT scores. C Schema: The NOTCH luciferase reporter. CBF1/RBPJκ/Suppressor of Hairless/Lag-1 (CSL) response element (RE) is fused upstream of the firefly luciferase gene. NICD Notch intracellular domain, Graph luciferase reporter assay in H441 cells co-transfected with luciferase reporter and shACBD3 or NOTCH1 NICD. n = 4. D WB analysis of ACBD3 and EGFP-ACBD3 proteins in H1299 cells transfectants. E NOTCH luciferase reporter assay. F , G , I qPCR analysis of mRNA levels of ACBD3 and NOTCH target genes in shACBD3-tranfected H441 ( F ) and A549 ( G ), and ACBD3-transfected H1299 ( I ) cells. n = 4. H , J , K WB analysis of SNAI1 and ACBD3 protein levels in lung cancer cells transfected with shACBD3 ( H ), ACBD3 expression vector ( J ), or shACBD3 and SNAI1 siRNA ( K ). L , M Transwell migration ( L ) and apoptosis ( M ) assays of cell transfectants in ( K ). N Working model: ACDB3 inhibits the NOTCH-SNAI1 axis to suppress cancer cell motility and metastasis. Data indicate the mean ± SD from a single experiment incorporating biological replicate samples ( n = 3, unless otherwise indicated) and are representative of at least 2 independent experiments. P -values were determined using two-tailed Student’s t-test (for F , G , I ), one-way ANOVA test (for A , E , L , M ).

    Journal: Oncogene

    Article Title: Dichotomous roles of ACBD3 in NSCLC growth and metastasis

    doi: 10.1038/s41388-025-03360-w

    Figure Lengend Snippet: A Boyden chamber transwell migration and invasion assays in H1299 cells transfected with EGFP-ACBD3 or EGFP-ACBD3 lacking the NUMB-binding domain (ΔNUMB). B Correlation between ACBD3, NUMB, NOTCHs, or NOTCH target genes mRNA levels and EMT scores. C Schema: The NOTCH luciferase reporter. CBF1/RBPJκ/Suppressor of Hairless/Lag-1 (CSL) response element (RE) is fused upstream of the firefly luciferase gene. NICD Notch intracellular domain, Graph luciferase reporter assay in H441 cells co-transfected with luciferase reporter and shACBD3 or NOTCH1 NICD. n = 4. D WB analysis of ACBD3 and EGFP-ACBD3 proteins in H1299 cells transfectants. E NOTCH luciferase reporter assay. F , G , I qPCR analysis of mRNA levels of ACBD3 and NOTCH target genes in shACBD3-tranfected H441 ( F ) and A549 ( G ), and ACBD3-transfected H1299 ( I ) cells. n = 4. H , J , K WB analysis of SNAI1 and ACBD3 protein levels in lung cancer cells transfected with shACBD3 ( H ), ACBD3 expression vector ( J ), or shACBD3 and SNAI1 siRNA ( K ). L , M Transwell migration ( L ) and apoptosis ( M ) assays of cell transfectants in ( K ). N Working model: ACDB3 inhibits the NOTCH-SNAI1 axis to suppress cancer cell motility and metastasis. Data indicate the mean ± SD from a single experiment incorporating biological replicate samples ( n = 3, unless otherwise indicated) and are representative of at least 2 independent experiments. P -values were determined using two-tailed Student’s t-test (for F , G , I ), one-way ANOVA test (for A , E , L , M ).

    Article Snippet: NOTCH activity was assessed using the Notch1 Pathway Reporter Kit (#79503, BPS Bioscience) following the manufacturer’s instructions.

    Techniques: Migration, Transfection, Binding Assay, Luciferase, Reporter Assay, Expressing, Plasmid Preparation, Two Tailed Test