qiazol  (Qiagen)


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    Name:
    QIAzol Lysis Reagent
    Description:
    For efficient lysis of fatty and standard tissues before RNA isolation Kit contents Qiagen QIAzol Lysis Reagent 200mL Silica Membrane Technology High Yield For Efficient Lysis of Fatty and Standard Tissues Before RNA Isolation Ideal for Downstream Application Benefits Optimized lysis conditions to purify RNA for gene expression analysis High yields of RNA from fatty tissues Easy to follow protocol for lysis and homogenization Integration with RNeasy cleanup to prevent phenol carryover Compatibility with a variety of tissue typ
    Catalog Number:
    79306
    Price:
    317
    Category:
    QIAzol Lysis Reagent
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    Structured Review

    Qiagen qiazol
    QIAzol Lysis Reagent
    For efficient lysis of fatty and standard tissues before RNA isolation Kit contents Qiagen QIAzol Lysis Reagent 200mL Silica Membrane Technology High Yield For Efficient Lysis of Fatty and Standard Tissues Before RNA Isolation Ideal for Downstream Application Benefits Optimized lysis conditions to purify RNA for gene expression analysis High yields of RNA from fatty tissues Easy to follow protocol for lysis and homogenization Integration with RNeasy cleanup to prevent phenol carryover Compatibility with a variety of tissue typ
    https://www.bioz.com/result/qiazol/product/Qiagen
    Average 90 stars, based on 749 article reviews
    Price from $9.99 to $1999.99
    qiazol - by Bioz Stars, 2020-01
    90/100 stars

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    Related Articles

    Amplification:

    Article Title: Myeloid Cell Responses to Contraction-induced Injury Differ in Muscles of Young and Old Mice
    Article Snippet: Muscle samples were homogenized in QIAzol lysis reagent (QIAGEN). .. RNA (2 µg) was reverse transcribed using a QuantiTect Reverse Transcription Kit (QIAGEN), and cDNA was diluted by fivefold and amplified in a CFX96 real time thermal cycler (Bio-Rad) using a QuantiTect SYBR Green I PCR Kit (QIAGEN) and primers specific for inducible nitric oxide synthase (iNOS) , tumor necrosis factor alpha (TNFα) , arginase-1 (Arg1) , interleukin-10 (IL-10) , and β2-microglobulin ( ).

    Article Title: Transcriptional Classification and Functional Characterization of Human Airway Macrophage and Dendritic Cell Subsets
    Article Snippet: Lung and monocyte-derived cell populations were sorted by FACS directly into QIAzol Lysis Reagent (QIAGEN) at ≤ 5 × 104 cells/600 μl and frozen at −80 °C. .. RNA was reverse transcribed to cDNA and amplified using Ovation Pico WTA Systems V2 (NuGEN) following manufacturer’s instructions.

    Quantitative RT-PCR:

    Article Title: Diagnostic Potential of Cell-Free and Exosomal MicroRNAs in the Identification of Patients with High-Risk Colorectal Adenomas
    Article Snippet: Paragraph title: Isolation of exosomes from serum, exosomal miRNA extraction and qRT-PCR ... Briefly, exosomes purified from 250 μL of serum were diluted with 500 μL of Qiazol Lysis Reagent (Qiagen).

    Article Title: Novel role and regulation of HDAC4 in cocaine-related behaviors
    Article Snippet: NAc punches were lysed in QIAzol lysis reagent (Qiagen Germantown, MD), and RNA was isolated using miRNAeasy collection kit (Qiagen). mRNA was converted to cDNA using SuperScriptIII-RT (Invitrogen Carlsbad, CA) and random hexamer primers. .. HDAC4 expression was quantified using RT-qPCR with probes Hdac4 5 ’ -CGAGCACAGAGGTGAAGATG-3 ’ (forward) and 5-GGTGGAGAGCTCTGGTCAA-3 ’ (reverse) and GAPDH 5 ’ -AGGTCGGTGTGAACGGATTTG-3 ’ (forward) and 5 ’ -TGTAGACCATGTAGTTGAGGTCA-3 ’ (reverse).

    Article Title: Oxidized phospholipids regulate amino acid metabolism through MTHFD2 to facilitate nucleotide release in endothelial cells
    Article Snippet: Paragraph title: Mouse partial carotid ligation and RNA isolation and qRT-PCR ... Briefly, LCA and RCA were quickly flushed with 150 μl of QIAzol lysis reagent (Qiagen) using 29G insulin syringe into a microfuge tube.

    Real-time Polymerase Chain Reaction:

    Article Title: Comparison of maternal isocaloric high carbohydrate and high fat diets on osteogenic and adipogenic genes expression in adolescent mice offspring
    Article Snippet: .. RNA isolation and real-time polymerase chain reaction (RT-PCR) Total RNA from the powdered samples were extracted using QIAzol Lysis Reagent (QIAGEN Inc., Valencia, CA 91355, USA) according to the manufacturer’s instructions, followed by DNase digestion and column cleanup using RNeasy mini columns (Qiagen, Valencia, CA, USA) [ ]. .. RNA quantification was assessed at 260 nm with a ND-1000 spectrophotometer (NanoDrop, Wilmington, DE, USA), and its quality (integrity) was checked by agarose gel electrophoresis.

    Article Title: Acute embryonic anoxia exposure favours the development of a dominant and aggressive phenotype in adult zebrafish
    Article Snippet: Total RNA was extracted from adult testis and ovary samples using QIAzol lysis reagent (Qiagen, Toronto, ON, Canada). .. Gonad mRNA levels of cyp19a were assessed using quantitative real-time PCR as previously described [ ].

    Article Title: Integration of Multi-omics Data from Mouse Diversity Panel Highlights Mitochondrial Dysfunction in Non-Alcoholic Fatty Liver Disease
    Article Snippet: Paragraph title: RNA isolation and quantitative polymerase chain reaction ... Total RNA was isolated from frozen mouse liver tissues using QIAzol Lysis Reagent (Qiagen, Germantown, MD) following manufacturer’s RNA isolation protocol.

    Article Title: Oxidized phospholipids regulate amino acid metabolism through MTHFD2 to facilitate nucleotide release in endothelial cells
    Article Snippet: Briefly, LCA and RCA were quickly flushed with 150 μl of QIAzol lysis reagent (Qiagen) using 29G insulin syringe into a microfuge tube. .. Total RNA was polyadenylated and reverse transcribed for use in a two-step qPCR setup using High-capacity cDNA synthesis kit (ABI) and using Brilliant II SYBR Green QPCR Master Mix (Stratagene) with custom-designed primers on a Real-Time PCR System (StepOne Plus, Applied Biosystems).

    Microarray:

    Article Title: Thrombin and interleukin-1? decrease HOX gene expression in human first trimester decidual cells: implications for pregnancy loss
    Article Snippet: Paragraph title: RNA isolation and microarray analysis ... Cells were harvested with QIAzol™ lysis reagent (Qiagen, Valencia, CA, USA) and used to prepare total RNA.

    Random Hexamer Labeling:

    Article Title: Novel role and regulation of HDAC4 in cocaine-related behaviors
    Article Snippet: .. NAc punches were lysed in QIAzol lysis reagent (Qiagen Germantown, MD), and RNA was isolated using miRNAeasy collection kit (Qiagen). mRNA was converted to cDNA using SuperScriptIII-RT (Invitrogen Carlsbad, CA) and random hexamer primers. .. HDAC4 expression was quantified using RT-qPCR with probes Hdac4 5 ’ -CGAGCACAGAGGTGAAGATG-3 ’ (forward) and 5-GGTGGAGAGCTCTGGTCAA-3 ’ (reverse) and GAPDH 5 ’ -AGGTCGGTGTGAACGGATTTG-3 ’ (forward) and 5 ’ -TGTAGACCATGTAGTTGAGGTCA-3 ’ (reverse).

    Expressing:

    Article Title: Fine mapping of a QTL on bovine chromosome 6 using imputed full sequence data suggests a key role for the group-specific component (GC) gene in clinical mastitis and milk production
    Article Snippet: Based on the genotypes for rs110675140, liver samples from ten unrelated bulls (five for each homozygous genotype) from randomly selected farms (in order to randomize the environmental effect) were selected and used for analysis of mRNA expression. .. RNA was isolated by using the QIAzol lysis reagent (Qiagen, Hilden, Germany) and on-column purification NucleoSpin RNA kit (Macherey–Nagel) with on-column DNase digestion according to the manufacturer’s instructions.

    Article Title: Acute embryonic anoxia exposure favours the development of a dominant and aggressive phenotype in adult zebrafish
    Article Snippet: Paragraph title: (i) Gene expression quantification ... Total RNA was extracted from adult testis and ovary samples using QIAzol lysis reagent (Qiagen, Toronto, ON, Canada).

    Article Title: Myeloid Cell Responses to Contraction-induced Injury Differ in Muscles of Young and Old Mice
    Article Snippet: Paragraph title: Gene Expression ... Muscle samples were homogenized in QIAzol lysis reagent (QIAGEN).

    Article Title: Cell death mechanisms of the anti-cancer drug etoposide on human cardiomyocytes isolated from pluripotent stem cells
    Article Snippet: .. To analyze the mRNA expression, cell samples were homogenized with QIAzol lysis reagent (QIAGEN, Hilden, Germany), and the total RNA was extracted using the miRNeasy Mini Kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions. .. Extracted RNA was assessed for purity and quantity using Nanodrop (ND-1000, Thermo Fisher, Germany).

    Article Title: LaeA regulation of secondary metabolism modulates virulence in Penicillium expansum and is mediated by sucrose
    Article Snippet: Paragraph title: Analysis of secondary metabolite backbone gene expression by semi‐quantitative RT‐PCR ... RNA from the strains grown in CY liquid medium was extracted using QIAzol® Lysis Reagent (Qiagen, Hilden, Germany) according to the manufacturer's instructions.

    Article Title: Novel role and regulation of HDAC4 in cocaine-related behaviors
    Article Snippet: NAc punches were lysed in QIAzol lysis reagent (Qiagen Germantown, MD), and RNA was isolated using miRNAeasy collection kit (Qiagen). mRNA was converted to cDNA using SuperScriptIII-RT (Invitrogen Carlsbad, CA) and random hexamer primers. .. HDAC4 expression was quantified using RT-qPCR with probes Hdac4 5 ’ -CGAGCACAGAGGTGAAGATG-3 ’ (forward) and 5-GGTGGAGAGCTCTGGTCAA-3 ’ (reverse) and GAPDH 5 ’ -AGGTCGGTGTGAACGGATTTG-3 ’ (forward) and 5 ’ -TGTAGACCATGTAGTTGAGGTCA-3 ’ (reverse).

    Article Title: Integration of Multi-omics Data from Mouse Diversity Panel Highlights Mitochondrial Dysfunction in Non-Alcoholic Fatty Liver Disease
    Article Snippet: Total RNA was isolated from frozen mouse liver tissues using QIAzol Lysis Reagent (Qiagen, Germantown, MD) following manufacturer’s RNA isolation protocol. .. Relative quantitative gene expression levels were measured by quantitative PCR using Kapa SYBR Fast qPCR kit (Kapa Biosystems, Inc., Wilmington, MA) on a LightCycler 480 II (Roche) and analyzed using the Roche LightCycler1.5.0 Software.

    Article Title: Thrombin and interleukin-1? decrease HOX gene expression in human first trimester decidual cells: implications for pregnancy loss
    Article Snippet: Microarray analysis was used to assess the expression of all HOX genes in response to treatment. .. Cells were harvested with QIAzol™ lysis reagent (Qiagen, Valencia, CA, USA) and used to prepare total RNA.

    Derivative Assay:

    Article Title: Diagnostic Potential of Cell-Free and Exosomal MicroRNAs in the Identification of Patients with High-Risk Colorectal Adenomas
    Article Snippet: Isolated exosomes from all serum using ExoQuick™ kit were well characterized by exosomal protein markers (CD63, CD9 and CD81) and not specific exosomes derived from colonic tissues. .. Briefly, exosomes purified from 250 μL of serum were diluted with 500 μL of Qiazol Lysis Reagent (Qiagen).

    Flow Cytometry:

    Article Title: Oxidized phospholipids regulate amino acid metabolism through MTHFD2 to facilitate nucleotide release in endothelial cells
    Article Snippet: Post procedure, d -flow in the LCA of all animals was confirmed by ultrasonography to verify the success of partial ligation surgery. .. Briefly, LCA and RCA were quickly flushed with 150 μl of QIAzol lysis reagent (Qiagen) using 29G insulin syringe into a microfuge tube.

    Ligation:

    Article Title: Oxidized phospholipids regulate amino acid metabolism through MTHFD2 to facilitate nucleotide release in endothelial cells
    Article Snippet: Paragraph title: Mouse partial carotid ligation and RNA isolation and qRT-PCR ... Briefly, LCA and RCA were quickly flushed with 150 μl of QIAzol lysis reagent (Qiagen) using 29G insulin syringe into a microfuge tube.

    SYBR Green Assay:

    Article Title: Comparison of maternal isocaloric high carbohydrate and high fat diets on osteogenic and adipogenic genes expression in adolescent mice offspring
    Article Snippet: RNA isolation and real-time polymerase chain reaction (RT-PCR) Total RNA from the powdered samples were extracted using QIAzol Lysis Reagent (QIAGEN Inc., Valencia, CA 91355, USA) according to the manufacturer’s instructions, followed by DNase digestion and column cleanup using RNeasy mini columns (Qiagen, Valencia, CA, USA) [ ]. .. The RT-PCR was performed in an ABI StepOne sequence detection system (Applied Biosystems, California, USA) with 1 μl of cDNA and 10 pmol of each primer corresponding to the tested genes, using the SYBR Green I Master Mix (Roche).

    Article Title: Myeloid Cell Responses to Contraction-induced Injury Differ in Muscles of Young and Old Mice
    Article Snippet: Muscle samples were homogenized in QIAzol lysis reagent (QIAGEN). .. RNA (2 µg) was reverse transcribed using a QuantiTect Reverse Transcription Kit (QIAGEN), and cDNA was diluted by fivefold and amplified in a CFX96 real time thermal cycler (Bio-Rad) using a QuantiTect SYBR Green I PCR Kit (QIAGEN) and primers specific for inducible nitric oxide synthase (iNOS) , tumor necrosis factor alpha (TNFα) , arginase-1 (Arg1) , interleukin-10 (IL-10) , and β2-microglobulin ( ).

    Article Title: Oxidized phospholipids regulate amino acid metabolism through MTHFD2 to facilitate nucleotide release in endothelial cells
    Article Snippet: Briefly, LCA and RCA were quickly flushed with 150 μl of QIAzol lysis reagent (Qiagen) using 29G insulin syringe into a microfuge tube. .. Total RNA was polyadenylated and reverse transcribed for use in a two-step qPCR setup using High-capacity cDNA synthesis kit (ABI) and using Brilliant II SYBR Green QPCR Master Mix (Stratagene) with custom-designed primers on a Real-Time PCR System (StepOne Plus, Applied Biosystems).

    Cell Culture:

    Article Title: LaeA regulation of secondary metabolism modulates virulence in Penicillium expansum and is mediated by sucrose
    Article Snippet: Each cellophane sheet was floated on the medium and cultured without shaking in the dark. .. RNA from the strains grown in CY liquid medium was extracted using QIAzol® Lysis Reagent (Qiagen, Hilden, Germany) according to the manufacturer's instructions.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Comparison of maternal isocaloric high carbohydrate and high fat diets on osteogenic and adipogenic genes expression in adolescent mice offspring
    Article Snippet: .. RNA isolation and real-time polymerase chain reaction (RT-PCR) Total RNA from the powdered samples were extracted using QIAzol Lysis Reagent (QIAGEN Inc., Valencia, CA 91355, USA) according to the manufacturer’s instructions, followed by DNase digestion and column cleanup using RNeasy mini columns (Qiagen, Valencia, CA, USA) [ ]. .. RNA quantification was assessed at 260 nm with a ND-1000 spectrophotometer (NanoDrop, Wilmington, DE, USA), and its quality (integrity) was checked by agarose gel electrophoresis.

    Article Title: Diagnostic Potential of Cell-Free and Exosomal MicroRNAs in the Identification of Patients with High-Risk Colorectal Adenomas
    Article Snippet: Briefly, exosomes purified from 250 μL of serum were diluted with 500 μL of Qiazol Lysis Reagent (Qiagen). .. For miRNA-based RT-PCR assays, 2 μL of enriched small RNAs from serum were reverse transcribed using the TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystems, San Diego, CA, USA) in a total volume of 15 μL.

    Article Title: LaeA regulation of secondary metabolism modulates virulence in Penicillium expansum and is mediated by sucrose
    Article Snippet: Paragraph title: Analysis of secondary metabolite backbone gene expression by semi‐quantitative RT‐PCR ... RNA from the strains grown in CY liquid medium was extracted using QIAzol® Lysis Reagent (Qiagen, Hilden, Germany) according to the manufacturer's instructions.

    Sequencing:

    Article Title: Comparison of maternal isocaloric high carbohydrate and high fat diets on osteogenic and adipogenic genes expression in adolescent mice offspring
    Article Snippet: RNA isolation and real-time polymerase chain reaction (RT-PCR) Total RNA from the powdered samples were extracted using QIAzol Lysis Reagent (QIAGEN Inc., Valencia, CA 91355, USA) according to the manufacturer’s instructions, followed by DNase digestion and column cleanup using RNeasy mini columns (Qiagen, Valencia, CA, USA) [ ]. .. The RT-PCR was performed in an ABI StepOne sequence detection system (Applied Biosystems, California, USA) with 1 μl of cDNA and 10 pmol of each primer corresponding to the tested genes, using the SYBR Green I Master Mix (Roche).

    Multiple Displacement Amplification:

    Article Title: mirRICH, a simple method to enrich the small RNA fraction from over-dried RNA pellets
    Article Snippet: .. Small RNA extraction method Culture the target cells (here, 5× 106 cells of the breast cancer cell line MDA-MB-231 and MCF7 for 3 days) and treat them with 1 mL QIAzol lysis reagent. ..

    Isolation:

    Article Title: Comparison of maternal isocaloric high carbohydrate and high fat diets on osteogenic and adipogenic genes expression in adolescent mice offspring
    Article Snippet: .. RNA isolation and real-time polymerase chain reaction (RT-PCR) Total RNA from the powdered samples were extracted using QIAzol Lysis Reagent (QIAGEN Inc., Valencia, CA 91355, USA) according to the manufacturer’s instructions, followed by DNase digestion and column cleanup using RNeasy mini columns (Qiagen, Valencia, CA, USA) [ ]. .. RNA quantification was assessed at 260 nm with a ND-1000 spectrophotometer (NanoDrop, Wilmington, DE, USA), and its quality (integrity) was checked by agarose gel electrophoresis.

    Article Title: Fine mapping of a QTL on bovine chromosome 6 using imputed full sequence data suggests a key role for the group-specific component (GC) gene in clinical mastitis and milk production
    Article Snippet: .. RNA was isolated by using the QIAzol lysis reagent (Qiagen, Hilden, Germany) and on-column purification NucleoSpin RNA kit (Macherey–Nagel) with on-column DNase digestion according to the manufacturer’s instructions. .. RNA concentration and quality were measured with a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, Wilmington) and Agilent RNA 6000 Nano LabChip on the 2100 Agilent BioAnalyzer (Agilent Technologies, Palo Alto), respectively.

    Article Title: Myeloid Cell Responses to Contraction-induced Injury Differ in Muscles of Young and Old Mice
    Article Snippet: Muscle samples were homogenized in QIAzol lysis reagent (QIAGEN). .. RNA was isolated using a miRNeasy Mini Kit and treated with DNase I (QIAGEN).

    Article Title: Diagnostic Potential of Cell-Free and Exosomal MicroRNAs in the Identification of Patients with High-Risk Colorectal Adenomas
    Article Snippet: Paragraph title: Isolation of exosomes from serum, exosomal miRNA extraction and qRT-PCR ... Briefly, exosomes purified from 250 μL of serum were diluted with 500 μL of Qiazol Lysis Reagent (Qiagen).

    Article Title: Signaling network between the dysregulated expression of microRNAs and mRNAs in propofol-induced developmental neurotoxicity in mice
    Article Snippet: Total RNA was isolated using the phenol-chloroform method as previously described . .. Briefly, after mouse hippocampal tissues were lysed in the QIAzol lysis reagent (Qiagen Inc., Valencia, CA), chloroform was thoroughly mixed with the samples, set for 5 minutes before centrifuged.

    Article Title: Novel role and regulation of HDAC4 in cocaine-related behaviors
    Article Snippet: .. NAc punches were lysed in QIAzol lysis reagent (Qiagen Germantown, MD), and RNA was isolated using miRNAeasy collection kit (Qiagen). mRNA was converted to cDNA using SuperScriptIII-RT (Invitrogen Carlsbad, CA) and random hexamer primers. .. HDAC4 expression was quantified using RT-qPCR with probes Hdac4 5 ’ -CGAGCACAGAGGTGAAGATG-3 ’ (forward) and 5-GGTGGAGAGCTCTGGTCAA-3 ’ (reverse) and GAPDH 5 ’ -AGGTCGGTGTGAACGGATTTG-3 ’ (forward) and 5 ’ -TGTAGACCATGTAGTTGAGGTCA-3 ’ (reverse).

    Article Title: Integration of Multi-omics Data from Mouse Diversity Panel Highlights Mitochondrial Dysfunction in Non-Alcoholic Fatty Liver Disease
    Article Snippet: .. Total RNA was isolated from frozen mouse liver tissues using QIAzol Lysis Reagent (Qiagen, Germantown, MD) following manufacturer’s RNA isolation protocol. .. First-strand complementary DNA (cDNA) was made from 2µg total RNA of each mouse according to the manufacturer’s protocol using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Waltham, MA).

    Article Title: Thrombin and interleukin-1? decrease HOX gene expression in human first trimester decidual cells: implications for pregnancy loss
    Article Snippet: Paragraph title: RNA isolation and microarray analysis ... Cells were harvested with QIAzol™ lysis reagent (Qiagen, Valencia, CA, USA) and used to prepare total RNA.

    Article Title: Oxidized phospholipids regulate amino acid metabolism through MTHFD2 to facilitate nucleotide release in endothelial cells
    Article Snippet: Paragraph title: Mouse partial carotid ligation and RNA isolation and qRT-PCR ... Briefly, LCA and RCA were quickly flushed with 150 μl of QIAzol lysis reagent (Qiagen) using 29G insulin syringe into a microfuge tube.

    Labeling:

    Article Title: Thrombin and interleukin-1? decrease HOX gene expression in human first trimester decidual cells: implications for pregnancy loss
    Article Snippet: Cells were harvested with QIAzol™ lysis reagent (Qiagen, Valencia, CA, USA) and used to prepare total RNA. .. Then GeneChip IVT Labeling Kit (Affymetrix Inc., Santa Clara, CA, USA) was used to generate biotinylated cRNA.

    Purification:

    Article Title: Fine mapping of a QTL on bovine chromosome 6 using imputed full sequence data suggests a key role for the group-specific component (GC) gene in clinical mastitis and milk production
    Article Snippet: .. RNA was isolated by using the QIAzol lysis reagent (Qiagen, Hilden, Germany) and on-column purification NucleoSpin RNA kit (Macherey–Nagel) with on-column DNase digestion according to the manufacturer’s instructions. .. RNA concentration and quality were measured with a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, Wilmington) and Agilent RNA 6000 Nano LabChip on the 2100 Agilent BioAnalyzer (Agilent Technologies, Palo Alto), respectively.

    Article Title: Diagnostic Potential of Cell-Free and Exosomal MicroRNAs in the Identification of Patients with High-Risk Colorectal Adenomas
    Article Snippet: .. Briefly, exosomes purified from 250 μL of serum were diluted with 500 μL of Qiazol Lysis Reagent (Qiagen). .. After 5 min of incubation at room temperature, 5 μL of 5 nM syn-cel-miR-39 miScript miRNA Mimic and 100μL chloroform were added to each sample followed by vortexing for 15 s. Subsequent extraction and cartridge work were carried out according to the manufacturer’s protocol, with the exception that the final RNA elution was performed with 40 μL of RNase-free water.

    Article Title: Transcriptional Classification and Functional Characterization of Human Airway Macrophage and Dendritic Cell Subsets
    Article Snippet: Lung and monocyte-derived cell populations were sorted by FACS directly into QIAzol Lysis Reagent (QIAGEN) at ≤ 5 × 104 cells/600 μl and frozen at −80 °C. .. When thawed, samples were purified using a QIAcube system in conjunction with miRNeasy Mini Kits (both from QIAGEN) per manufacturer’s instructions.

    Article Title: Thrombin and interleukin-1? decrease HOX gene expression in human first trimester decidual cells: implications for pregnancy loss
    Article Snippet: Cells were harvested with QIAzol™ lysis reagent (Qiagen, Valencia, CA, USA) and used to prepare total RNA. .. A T7-(dT)24 oligo-primer was used to synthesize double-stranded cDNA by the One-Cycle cDNA Synthesis Kit (Affymetrix Inc., Santa Clara, CA, USA) which was subsequently purified using Sample Cleanup Module (Affymetrix Inc., Santa Clara, CA, USA) and ethanol precipitation.

    Polymerase Chain Reaction:

    Article Title: Myeloid Cell Responses to Contraction-induced Injury Differ in Muscles of Young and Old Mice
    Article Snippet: Muscle samples were homogenized in QIAzol lysis reagent (QIAGEN). .. RNA (2 µg) was reverse transcribed using a QuantiTect Reverse Transcription Kit (QIAGEN), and cDNA was diluted by fivefold and amplified in a CFX96 real time thermal cycler (Bio-Rad) using a QuantiTect SYBR Green I PCR Kit (QIAGEN) and primers specific for inducible nitric oxide synthase (iNOS) , tumor necrosis factor alpha (TNFα) , arginase-1 (Arg1) , interleukin-10 (IL-10) , and β2-microglobulin ( ).

    Article Title: Diagnostic Potential of Cell-Free and Exosomal MicroRNAs in the Identification of Patients with High-Risk Colorectal Adenomas
    Article Snippet: Briefly, exosomes purified from 250 μL of serum were diluted with 500 μL of Qiazol Lysis Reagent (Qiagen). .. PCR reactions for quantification of miR-21, miR-29a, miR-92a, miR-135b, and cel-miR-39 were performed in duplicate using TaqMan 2× Universal PCR Master Mix according to the manufacturer’s protocol.

    Article Title: Cell death mechanisms of the anti-cancer drug etoposide on human cardiomyocytes isolated from pluripotent stem cells
    Article Snippet: To analyze the mRNA expression, cell samples were homogenized with QIAzol lysis reagent (QIAGEN, Hilden, Germany), and the total RNA was extracted using the miRNeasy Mini Kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions. .. Quantitative comparison of mRNA levels was performed using custom made RT2 Profiler PCR array (96-well plate) (Qiagen, Arch Toxicol Hilden, Germany) containing 84 target genes, 5 housekeeping genes, 1 genomic DNA control, 3 reverse transcription controls, and 3 positive PCR controls (all 84 target genes are shown in Supplementary Table No. 1).

    Article Title: LaeA regulation of secondary metabolism modulates virulence in Penicillium expansum and is mediated by sucrose
    Article Snippet: RNA from the strains grown in CY liquid medium was extracted using QIAzol® Lysis Reagent (Qiagen, Hilden, Germany) according to the manufacturer's instructions. .. RNA (10 μg) was treated with DNase I (New England BioLabs, Ipswich, MA, USA) and employed as template for cDNA synthesis using the iScriptTM cDNA Synthesis Kit (Bio‐Rad, Hercules, CA, USA). cDNA (50 ng) was used as the template DNA for semi‐quantitative PCR.

    FACS:

    Article Title: Transcriptional Classification and Functional Characterization of Human Airway Macrophage and Dendritic Cell Subsets
    Article Snippet: .. Lung and monocyte-derived cell populations were sorted by FACS directly into QIAzol Lysis Reagent (QIAGEN) at ≤ 5 × 104 cells/600 μl and frozen at −80 °C. .. When thawed, samples were purified using a QIAcube system in conjunction with miRNeasy Mini Kits (both from QIAGEN) per manufacturer’s instructions.

    Mouse Assay:

    Article Title: Oxidized phospholipids regulate amino acid metabolism through MTHFD2 to facilitate nucleotide release in endothelial cells
    Article Snippet: Mice were monitored post-surgery and were allowed to recover. .. Briefly, LCA and RCA were quickly flushed with 150 μl of QIAzol lysis reagent (Qiagen) using 29G insulin syringe into a microfuge tube.

    Software:

    Article Title: Integration of Multi-omics Data from Mouse Diversity Panel Highlights Mitochondrial Dysfunction in Non-Alcoholic Fatty Liver Disease
    Article Snippet: Total RNA was isolated from frozen mouse liver tissues using QIAzol Lysis Reagent (Qiagen, Germantown, MD) following manufacturer’s RNA isolation protocol. .. Relative quantitative gene expression levels were measured by quantitative PCR using Kapa SYBR Fast qPCR kit (Kapa Biosystems, Inc., Wilmington, MA) on a LightCycler 480 II (Roche) and analyzed using the Roche LightCycler1.5.0 Software.

    Electrophoresis:

    Article Title: Acute embryonic anoxia exposure favours the development of a dominant and aggressive phenotype in adult zebrafish
    Article Snippet: Total RNA was extracted from adult testis and ovary samples using QIAzol lysis reagent (Qiagen, Toronto, ON, Canada). .. The concentration of total RNA was quantified, using a NanoDrop 8000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA), and RNA quality was assessed via electrophoresis using the 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA).

    RNA Extraction:

    Article Title: Signaling network between the dysregulated expression of microRNAs and mRNAs in propofol-induced developmental neurotoxicity in mice
    Article Snippet: Paragraph title: RNA extraction ... Briefly, after mouse hippocampal tissues were lysed in the QIAzol lysis reagent (Qiagen Inc., Valencia, CA), chloroform was thoroughly mixed with the samples, set for 5 minutes before centrifuged.

    Article Title: Novel role and regulation of HDAC4 in cocaine-related behaviors
    Article Snippet: Bilateral punches were taken using a 1-mm stainless steel punch tool and placed in cold microcentrifuge tubes for later RNA extraction. .. NAc punches were lysed in QIAzol lysis reagent (Qiagen Germantown, MD), and RNA was isolated using miRNAeasy collection kit (Qiagen). mRNA was converted to cDNA using SuperScriptIII-RT (Invitrogen Carlsbad, CA) and random hexamer primers.

    Article Title: mirRICH, a simple method to enrich the small RNA fraction from over-dried RNA pellets
    Article Snippet: .. Small RNA extraction method Culture the target cells (here, 5× 106 cells of the breast cancer cell line MDA-MB-231 and MCF7 for 3 days) and treat them with 1 mL QIAzol lysis reagent. ..

    Agarose Gel Electrophoresis:

    Article Title: Comparison of maternal isocaloric high carbohydrate and high fat diets on osteogenic and adipogenic genes expression in adolescent mice offspring
    Article Snippet: RNA isolation and real-time polymerase chain reaction (RT-PCR) Total RNA from the powdered samples were extracted using QIAzol Lysis Reagent (QIAGEN Inc., Valencia, CA 91355, USA) according to the manufacturer’s instructions, followed by DNase digestion and column cleanup using RNeasy mini columns (Qiagen, Valencia, CA, USA) [ ]. .. RNA quantification was assessed at 260 nm with a ND-1000 spectrophotometer (NanoDrop, Wilmington, DE, USA), and its quality (integrity) was checked by agarose gel electrophoresis.

    In Vitro:

    Article Title: Thrombin and interleukin-1? decrease HOX gene expression in human first trimester decidual cells: implications for pregnancy loss
    Article Snippet: Each in vitro experimental condition was repeated three times and in triplicate; each repetition used cells obtained from separate individuals. .. Cells were harvested with QIAzol™ lysis reagent (Qiagen, Valencia, CA, USA) and used to prepare total RNA.

    Ethanol Precipitation:

    Article Title: Thrombin and interleukin-1? decrease HOX gene expression in human first trimester decidual cells: implications for pregnancy loss
    Article Snippet: Cells were harvested with QIAzol™ lysis reagent (Qiagen, Valencia, CA, USA) and used to prepare total RNA. .. A T7-(dT)24 oligo-primer was used to synthesize double-stranded cDNA by the One-Cycle cDNA Synthesis Kit (Affymetrix Inc., Santa Clara, CA, USA) which was subsequently purified using Sample Cleanup Module (Affymetrix Inc., Santa Clara, CA, USA) and ethanol precipitation.

    Incubation:

    Article Title: Diagnostic Potential of Cell-Free and Exosomal MicroRNAs in the Identification of Patients with High-Risk Colorectal Adenomas
    Article Snippet: Next, 250 μL of the supernatant was mixed with 63 μL of ExoQuick™ solution and incubated at 4°C for 30 minutes. .. Briefly, exosomes purified from 250 μL of serum were diluted with 500 μL of Qiazol Lysis Reagent (Qiagen).

    Spectrophotometry:

    Article Title: Comparison of maternal isocaloric high carbohydrate and high fat diets on osteogenic and adipogenic genes expression in adolescent mice offspring
    Article Snippet: RNA isolation and real-time polymerase chain reaction (RT-PCR) Total RNA from the powdered samples were extracted using QIAzol Lysis Reagent (QIAGEN Inc., Valencia, CA 91355, USA) according to the manufacturer’s instructions, followed by DNase digestion and column cleanup using RNeasy mini columns (Qiagen, Valencia, CA, USA) [ ]. .. RNA quantification was assessed at 260 nm with a ND-1000 spectrophotometer (NanoDrop, Wilmington, DE, USA), and its quality (integrity) was checked by agarose gel electrophoresis.

    Article Title: Fine mapping of a QTL on bovine chromosome 6 using imputed full sequence data suggests a key role for the group-specific component (GC) gene in clinical mastitis and milk production
    Article Snippet: RNA was isolated by using the QIAzol lysis reagent (Qiagen, Hilden, Germany) and on-column purification NucleoSpin RNA kit (Macherey–Nagel) with on-column DNase digestion according to the manufacturer’s instructions. .. RNA concentration and quality were measured with a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, Wilmington) and Agilent RNA 6000 Nano LabChip on the 2100 Agilent BioAnalyzer (Agilent Technologies, Palo Alto), respectively.

    Article Title: Acute embryonic anoxia exposure favours the development of a dominant and aggressive phenotype in adult zebrafish
    Article Snippet: Total RNA was extracted from adult testis and ovary samples using QIAzol lysis reagent (Qiagen, Toronto, ON, Canada). .. The concentration of total RNA was quantified, using a NanoDrop 8000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA), and RNA quality was assessed via electrophoresis using the 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA).

    Article Title: Myeloid Cell Responses to Contraction-induced Injury Differ in Muscles of Young and Old Mice
    Article Snippet: Muscle samples were homogenized in QIAzol lysis reagent (QIAGEN). .. RNA concentration was measured with a NanoDrop 2000 Spectrophotometer (Thermo Scientific).

    Article Title: Signaling network between the dysregulated expression of microRNAs and mRNAs in propofol-induced developmental neurotoxicity in mice
    Article Snippet: Briefly, after mouse hippocampal tissues were lysed in the QIAzol lysis reagent (Qiagen Inc., Valencia, CA), chloroform was thoroughly mixed with the samples, set for 5 minutes before centrifuged. .. The quantity and purity of RNA were validated using a Nanodrop spectrophotometer (Thermo scientific, Waltham, MA, US).

    Concentration Assay:

    Article Title: Fine mapping of a QTL on bovine chromosome 6 using imputed full sequence data suggests a key role for the group-specific component (GC) gene in clinical mastitis and milk production
    Article Snippet: RNA was isolated by using the QIAzol lysis reagent (Qiagen, Hilden, Germany) and on-column purification NucleoSpin RNA kit (Macherey–Nagel) with on-column DNase digestion according to the manufacturer’s instructions. .. RNA concentration and quality were measured with a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, Wilmington) and Agilent RNA 6000 Nano LabChip on the 2100 Agilent BioAnalyzer (Agilent Technologies, Palo Alto), respectively.

    Article Title: Acute embryonic anoxia exposure favours the development of a dominant and aggressive phenotype in adult zebrafish
    Article Snippet: Total RNA was extracted from adult testis and ovary samples using QIAzol lysis reagent (Qiagen, Toronto, ON, Canada). .. The concentration of total RNA was quantified, using a NanoDrop 8000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA), and RNA quality was assessed via electrophoresis using the 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA).

    Article Title: Myeloid Cell Responses to Contraction-induced Injury Differ in Muscles of Young and Old Mice
    Article Snippet: Muscle samples were homogenized in QIAzol lysis reagent (QIAGEN). .. RNA concentration was measured with a NanoDrop 2000 Spectrophotometer (Thermo Scientific).

    Article Title: LaeA regulation of secondary metabolism modulates virulence in Penicillium expansum and is mediated by sucrose
    Article Snippet: RNA from the strains grown in CY liquid medium was extracted using QIAzol® Lysis Reagent (Qiagen, Hilden, Germany) according to the manufacturer's instructions. .. RNA purity and concentration were determined with an Epoch 2 microplate reader (Biotek, Winooski, VT, USA).

    Lysis:

    Article Title: Comparison of maternal isocaloric high carbohydrate and high fat diets on osteogenic and adipogenic genes expression in adolescent mice offspring
    Article Snippet: .. RNA isolation and real-time polymerase chain reaction (RT-PCR) Total RNA from the powdered samples were extracted using QIAzol Lysis Reagent (QIAGEN Inc., Valencia, CA 91355, USA) according to the manufacturer’s instructions, followed by DNase digestion and column cleanup using RNeasy mini columns (Qiagen, Valencia, CA, USA) [ ]. .. RNA quantification was assessed at 260 nm with a ND-1000 spectrophotometer (NanoDrop, Wilmington, DE, USA), and its quality (integrity) was checked by agarose gel electrophoresis.

    Article Title: Fine mapping of a QTL on bovine chromosome 6 using imputed full sequence data suggests a key role for the group-specific component (GC) gene in clinical mastitis and milk production
    Article Snippet: .. RNA was isolated by using the QIAzol lysis reagent (Qiagen, Hilden, Germany) and on-column purification NucleoSpin RNA kit (Macherey–Nagel) with on-column DNase digestion according to the manufacturer’s instructions. .. RNA concentration and quality were measured with a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, Wilmington) and Agilent RNA 6000 Nano LabChip on the 2100 Agilent BioAnalyzer (Agilent Technologies, Palo Alto), respectively.

    Article Title: Acute embryonic anoxia exposure favours the development of a dominant and aggressive phenotype in adult zebrafish
    Article Snippet: .. Total RNA was extracted from adult testis and ovary samples using QIAzol lysis reagent (Qiagen, Toronto, ON, Canada). .. The concentration of total RNA was quantified, using a NanoDrop 8000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA), and RNA quality was assessed via electrophoresis using the 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA).

    Article Title: Myeloid Cell Responses to Contraction-induced Injury Differ in Muscles of Young and Old Mice
    Article Snippet: .. Muscle samples were homogenized in QIAzol lysis reagent (QIAGEN). .. RNA was isolated using a miRNeasy Mini Kit and treated with DNase I (QIAGEN).

    Article Title: Diagnostic Potential of Cell-Free and Exosomal MicroRNAs in the Identification of Patients with High-Risk Colorectal Adenomas
    Article Snippet: .. Briefly, exosomes purified from 250 μL of serum were diluted with 500 μL of Qiazol Lysis Reagent (Qiagen). .. After 5 min of incubation at room temperature, 5 μL of 5 nM syn-cel-miR-39 miScript miRNA Mimic and 100μL chloroform were added to each sample followed by vortexing for 15 s. Subsequent extraction and cartridge work were carried out according to the manufacturer’s protocol, with the exception that the final RNA elution was performed with 40 μL of RNase-free water.

    Article Title: Cell death mechanisms of the anti-cancer drug etoposide on human cardiomyocytes isolated from pluripotent stem cells
    Article Snippet: .. To analyze the mRNA expression, cell samples were homogenized with QIAzol lysis reagent (QIAGEN, Hilden, Germany), and the total RNA was extracted using the miRNeasy Mini Kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions. .. Extracted RNA was assessed for purity and quantity using Nanodrop (ND-1000, Thermo Fisher, Germany).

    Article Title: Signaling network between the dysregulated expression of microRNAs and mRNAs in propofol-induced developmental neurotoxicity in mice
    Article Snippet: .. Briefly, after mouse hippocampal tissues were lysed in the QIAzol lysis reagent (Qiagen Inc., Valencia, CA), chloroform was thoroughly mixed with the samples, set for 5 minutes before centrifuged. ..

    Article Title: Transcriptional Classification and Functional Characterization of Human Airway Macrophage and Dendritic Cell Subsets
    Article Snippet: .. Lung and monocyte-derived cell populations were sorted by FACS directly into QIAzol Lysis Reagent (QIAGEN) at ≤ 5 × 104 cells/600 μl and frozen at −80 °C. .. When thawed, samples were purified using a QIAcube system in conjunction with miRNeasy Mini Kits (both from QIAGEN) per manufacturer’s instructions.

    Article Title: Mutual enhancement of differentiation of osteoblasts and osteocytes occurs through direct cell-cell contact
    Article Snippet: .. The cells on the apical surface of the membrane were then harvested in 700 μL QIAzol Lysis Reagent. .. The total cellular RNA was isolated using the RNeasy Mini Kit (Qiagen).

    Article Title: LaeA regulation of secondary metabolism modulates virulence in Penicillium expansum and is mediated by sucrose
    Article Snippet: .. RNA from the strains grown in CY liquid medium was extracted using QIAzol® Lysis Reagent (Qiagen, Hilden, Germany) according to the manufacturer's instructions. .. RNA from the strains grown on APAM was prepared with the RNeasy Mini Kit (Qiagen) as described by Tannous et al . ( ).

    Article Title: Integration of Multi-omics Data from Mouse Diversity Panel Highlights Mitochondrial Dysfunction in Non-Alcoholic Fatty Liver Disease
    Article Snippet: .. Total RNA was isolated from frozen mouse liver tissues using QIAzol Lysis Reagent (Qiagen, Germantown, MD) following manufacturer’s RNA isolation protocol. .. First-strand complementary DNA (cDNA) was made from 2µg total RNA of each mouse according to the manufacturer’s protocol using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Waltham, MA).

    Article Title: Thrombin and interleukin-1? decrease HOX gene expression in human first trimester decidual cells: implications for pregnancy loss
    Article Snippet: .. Cells were harvested with QIAzol™ lysis reagent (Qiagen, Valencia, CA, USA) and used to prepare total RNA. .. According to the manufacturer's instruction, 100 µg of total RNA was precipitated using RNeasy Mini Kit (Qiagen, Valencia, CA, USA) and used as a template for cDNA synthesis.

    Article Title: mirRICH, a simple method to enrich the small RNA fraction from over-dried RNA pellets
    Article Snippet: .. Small RNA extraction method Culture the target cells (here, 5× 106 cells of the breast cancer cell line MDA-MB-231 and MCF7 for 3 days) and treat them with 1 mL QIAzol lysis reagent. ..

    Article Title: Oxidized phospholipids regulate amino acid metabolism through MTHFD2 to facilitate nucleotide release in endothelial cells
    Article Snippet: .. Briefly, LCA and RCA were quickly flushed with 150 μl of QIAzol lysis reagent (Qiagen) using 29G insulin syringe into a microfuge tube. .. The eluate was then used for total intimal RNA isolation using the miRNeasy mini kit (Qiagen) following the manufacturer’s protocol.

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    Qiagen qiazol lysis reagent
    Qiazol Lysis Reagent, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 872 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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