qiazol  (Qiagen)


Bioz Verified Symbol Qiagen is a verified supplier
Bioz Manufacturer Symbol Qiagen manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    Name:
    QIAzol Lysis Reagent
    Description:
    For efficient lysis of fatty and standard tissues before RNA isolation Kit contents Qiagen QIAzol Lysis Reagent 200mL Silica Membrane Technology High Yield For Efficient Lysis of Fatty and Standard Tissues Before RNA Isolation Ideal for Downstream Application Benefits Optimized lysis conditions to purify RNA for gene expression analysis High yields of RNA from fatty tissues Easy to follow protocol for lysis and homogenization Integration with RNeasy cleanup to prevent phenol carryover Compatibility with a variety of tissue typ
    Catalog Number:
    79306
    Price:
    317
    Category:
    QIAzol Lysis Reagent
    Buy from Supplier


    Structured Review

    Qiagen qiazol
    QIAzol Lysis Reagent
    For efficient lysis of fatty and standard tissues before RNA isolation Kit contents Qiagen QIAzol Lysis Reagent 200mL Silica Membrane Technology High Yield For Efficient Lysis of Fatty and Standard Tissues Before RNA Isolation Ideal for Downstream Application Benefits Optimized lysis conditions to purify RNA for gene expression analysis High yields of RNA from fatty tissues Easy to follow protocol for lysis and homogenization Integration with RNeasy cleanup to prevent phenol carryover Compatibility with a variety of tissue typ
    https://www.bioz.com/result/qiazol/product/Qiagen
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    qiazol - by Bioz Stars, 2021-03
    97/100 stars

    Images

    1) Product Images from "Temporal Progression of Alzheimer’s disease in Brains and Intestines of Transgenic Mice"

    Article Title: Temporal Progression of Alzheimer’s disease in Brains and Intestines of Transgenic Mice

    Journal: Neurobiology of aging

    doi: 10.1016/j.neurobiolaging.2019.05.025

    APP/PS1 and App NL-G-F colons had elevated cytokine mRNA levels in the colons of male mice. Flash frozen colons from WT, APP/PS1 and App NL-G-F female ( A ) and male ( B ) mice were lysed in QIAzol and RNA isolated. Real time PCR for TNF-α, IL-6 and IL-1β from samples were performed and fold change in mRNA expression were calculated as 2^-ΔΔCt, averaged and plotted ±SEM (n=5). Two-way ANOVA multiple comparisons indicate F 4,36 =4.703 and p=0.0037 (female, TNF-α), F 4,34 =2.573, p=0.0553 (female, IL-6), F 4,36 =1.108, p=0.3679 (female, IL-1 β), F 4,34 =2.018, p=0.1139 (male, TNF-α), F 4,35 =1.044, p=0.3987 (male, IL-6), and F 4,36 =2.058, p=0.1068 (male, IL-1β) between all interactions of age and strain. Multiple comparisons correction was determined by the Holm-Sidak method, *p
    Figure Legend Snippet: APP/PS1 and App NL-G-F colons had elevated cytokine mRNA levels in the colons of male mice. Flash frozen colons from WT, APP/PS1 and App NL-G-F female ( A ) and male ( B ) mice were lysed in QIAzol and RNA isolated. Real time PCR for TNF-α, IL-6 and IL-1β from samples were performed and fold change in mRNA expression were calculated as 2^-ΔΔCt, averaged and plotted ±SEM (n=5). Two-way ANOVA multiple comparisons indicate F 4,36 =4.703 and p=0.0037 (female, TNF-α), F 4,34 =2.573, p=0.0553 (female, IL-6), F 4,36 =1.108, p=0.3679 (female, IL-1 β), F 4,34 =2.018, p=0.1139 (male, TNF-α), F 4,35 =1.044, p=0.3987 (male, IL-6), and F 4,36 =2.058, p=0.1068 (male, IL-1β) between all interactions of age and strain. Multiple comparisons correction was determined by the Holm-Sidak method, *p

    Techniques Used: Mouse Assay, Isolation, Real-time Polymerase Chain Reaction, Expressing

    APP/PS1 and App NL-G-F had elevated cytokine mRNA levels in temporal cortices compared to WT controls. Temporal cortices from WT, APP/PS1 and App NL-G-F female ( A ) and male ( B ) mice were lysed in QIAzol and RNA isolated. Real time PCR for TNF-α, IL-6 and IL-1β from samples were performed and fold change in mRNA expression were calculated as 2^-ΔΔCt, averaged and plotted ±SEM (n=5). Two-way ANOVA multiple comparisons indicate F 4,30 =2.484 and p=0.064 (female, TNF-α), F 4,31 =3.37, p=0.0211 (female, IL-6), F 4,31 =2.113, p=0.1029 (female, IL-1β), F 4,34 =3.446, p=0.0181 (male, TNF-α), F 4,34 =1.507, p=0.222 (male, IL-6), and F 4,34 =4.476, p=0.005 (male, IL-1β) between all interactions of age and strain. Multiple comparisons correction was determined by the Holm-Sidak method, *p
    Figure Legend Snippet: APP/PS1 and App NL-G-F had elevated cytokine mRNA levels in temporal cortices compared to WT controls. Temporal cortices from WT, APP/PS1 and App NL-G-F female ( A ) and male ( B ) mice were lysed in QIAzol and RNA isolated. Real time PCR for TNF-α, IL-6 and IL-1β from samples were performed and fold change in mRNA expression were calculated as 2^-ΔΔCt, averaged and plotted ±SEM (n=5). Two-way ANOVA multiple comparisons indicate F 4,30 =2.484 and p=0.064 (female, TNF-α), F 4,31 =3.37, p=0.0211 (female, IL-6), F 4,31 =2.113, p=0.1029 (female, IL-1β), F 4,34 =3.446, p=0.0181 (male, TNF-α), F 4,34 =1.507, p=0.222 (male, IL-6), and F 4,34 =4.476, p=0.005 (male, IL-1β) between all interactions of age and strain. Multiple comparisons correction was determined by the Holm-Sidak method, *p

    Techniques Used: Mouse Assay, Isolation, Real-time Polymerase Chain Reaction, Expressing

    2) Product Images from "Controlling technical variation amongst 6693 patient microarrays of the randomized MINDACT trial"

    Article Title: Controlling technical variation amongst 6693 patient microarrays of the randomized MINDACT trial

    Journal: Communications Biology

    doi: 10.1038/s42003-020-1111-1

    MINDACT timeline and full-transcriptome dataset analysis procedure. a contains a timeline overview of the MINDACT enrollment and gene expression microarray technical adjustments and improvements. The MINDACT trial enrolled 6693 patients from February 2007 until September 2011 and samples were processed at Agendia’s central laboratory. During this time, some standard operating procedures underwent FDA/QSR-compliant technical adjustments and improvements. Evaluation and calibration were performed to ensure accuracy of the MammaPrint test. The technical changes listed here were the main factors included in the assessment of variance on full-transcriptome data of the MINDACT samples, further detail and other factors evaluated are described in detail in the “Methods” section and Supplementary Table 1 . The x -axis on top represents the years and the number of days between January 1st of 2007 (reference date) and the isolation date of the sample. This reference date was chosen to make the plots easier to read. Indicated are the first day (55) and the last day (1719) of patient sample isolation. Isolation buffer: commercial solution to extract RNA from (tumor) tissue, RNAbee (Teltest, TX, USA), and Qiazol (Qiagen, Germany), respectively. Reference is a standard RNA sample hybridized to the microarray together with each tumor RNA sample. Three different ones were used: MRP, Mamma Reference Pool, and BP1, Breast Pool 1, both made by Agendia from RNA isolated from a custom series of breast cancer samples and UHR, Universal Human Reference, a commercial RNA from human tumor reference samples (Agilent, USA). b shows the step-wise procedure of the MINDACT analysis pipeline for evaluating and managing technical variation in the gene expression levels representing the full-transcriptome of the MINDACT patients. For more detail see “Methods” and Supplementary Table 1 . ER estrogen receptor, HER2 erb-b2 receptor tyrosine kinase 2, IQR interquartile range, knn k-nearest neighbor, n number, RUV remove unwanted variation, TIFF tagged image file format, QC quality control.
    Figure Legend Snippet: MINDACT timeline and full-transcriptome dataset analysis procedure. a contains a timeline overview of the MINDACT enrollment and gene expression microarray technical adjustments and improvements. The MINDACT trial enrolled 6693 patients from February 2007 until September 2011 and samples were processed at Agendia’s central laboratory. During this time, some standard operating procedures underwent FDA/QSR-compliant technical adjustments and improvements. Evaluation and calibration were performed to ensure accuracy of the MammaPrint test. The technical changes listed here were the main factors included in the assessment of variance on full-transcriptome data of the MINDACT samples, further detail and other factors evaluated are described in detail in the “Methods” section and Supplementary Table 1 . The x -axis on top represents the years and the number of days between January 1st of 2007 (reference date) and the isolation date of the sample. This reference date was chosen to make the plots easier to read. Indicated are the first day (55) and the last day (1719) of patient sample isolation. Isolation buffer: commercial solution to extract RNA from (tumor) tissue, RNAbee (Teltest, TX, USA), and Qiazol (Qiagen, Germany), respectively. Reference is a standard RNA sample hybridized to the microarray together with each tumor RNA sample. Three different ones were used: MRP, Mamma Reference Pool, and BP1, Breast Pool 1, both made by Agendia from RNA isolated from a custom series of breast cancer samples and UHR, Universal Human Reference, a commercial RNA from human tumor reference samples (Agilent, USA). b shows the step-wise procedure of the MINDACT analysis pipeline for evaluating and managing technical variation in the gene expression levels representing the full-transcriptome of the MINDACT patients. For more detail see “Methods” and Supplementary Table 1 . ER estrogen receptor, HER2 erb-b2 receptor tyrosine kinase 2, IQR interquartile range, knn k-nearest neighbor, n number, RUV remove unwanted variation, TIFF tagged image file format, QC quality control.

    Techniques Used: Expressing, Microarray, Isolation

    3) Product Images from "Transfer of extracellular vesicle‐micro RNA controls germinal center reaction and antibody production"

    Article Title: Transfer of extracellular vesicle‐micro RNA controls germinal center reaction and antibody production

    Journal: EMBO Reports

    doi: 10.15252/embr.201948925

    Mmu‐miR‐20a‐5p, mmu‐miR‐25‐3p, and mmu‐miR‐155‐3p are detected in OT‐II CD4+ T cell‐derived EVs Mean counts per million (CPM) analyzed by small RNA NGS of the 6 differentially expressed miRNAs after IS formation in cell and EV fractions of mouse lymphoblast cultures. Bar charts show the mean of three independent experiments ± SEM. Significance was assessed by unpaired Student's t test comparing the exosomal and cellular miRNA content; * P
    Figure Legend Snippet: Mmu‐miR‐20a‐5p, mmu‐miR‐25‐3p, and mmu‐miR‐155‐3p are detected in OT‐II CD4+ T cell‐derived EVs Mean counts per million (CPM) analyzed by small RNA NGS of the 6 differentially expressed miRNAs after IS formation in cell and EV fractions of mouse lymphoblast cultures. Bar charts show the mean of three independent experiments ± SEM. Significance was assessed by unpaired Student's t test comparing the exosomal and cellular miRNA content; * P

    Techniques Used: Derivative Assay, Next-Generation Sequencing

    4) Product Images from "Identification of the miRNA targetome in hippocampal neurons using RIP-seq"

    Article Title: Identification of the miRNA targetome in hippocampal neurons using RIP-seq

    Journal: Scientific Reports

    doi: 10.1038/srep12609

    Establishment of neuron-specific RIP in the mouse brain ( A ) Diagram of the AAV5-GFP-AGO2 vector. ( B ) Diagram of the procedure of RNA-interacting protein immunoprecipitation (RIP-seq) ( C ) The expression of GFP-AGO2 is shown in low (left panel) and high (right panel) magnification. Scale bars 100 μm (left panel) and 10 μm (right panel). hpc- hippocampus ( D–F ) RNA from the hippocampus of was analysed using qPCR after RIP. Data are represented as mean ± SEM. ( D ) miRNAs such as miR-124 and miR-103 were detected only in RIP samples where GFP-AGO2 was expressed (****p
    Figure Legend Snippet: Establishment of neuron-specific RIP in the mouse brain ( A ) Diagram of the AAV5-GFP-AGO2 vector. ( B ) Diagram of the procedure of RNA-interacting protein immunoprecipitation (RIP-seq) ( C ) The expression of GFP-AGO2 is shown in low (left panel) and high (right panel) magnification. Scale bars 100 μm (left panel) and 10 μm (right panel). hpc- hippocampus ( D–F ) RNA from the hippocampus of was analysed using qPCR after RIP. Data are represented as mean ± SEM. ( D ) miRNAs such as miR-124 and miR-103 were detected only in RIP samples where GFP-AGO2 was expressed (****p

    Techniques Used: Plasmid Preparation, Immunoprecipitation, Expressing, Real-time Polymerase Chain Reaction

    5) Product Images from "Generation and characterization of a mitotane-resistant adrenocortical cell line"

    Article Title: Generation and characterization of a mitotane-resistant adrenocortical cell line

    Journal: Endocrine Connections

    doi: 10.1530/EC-19-0510

    (A) Copy number changes compared to founder cell line. Whole-exome sequencing data of resistant and nonresistant cell lines were compared to the founder cell line in a tumor/normal matched pair fashion to analyze copy number changes. Log2 fold changes are shown across the genome. Resistant cell lines share CNV patterns that are distinct from the control, suggesting a common cell of origin of resistant clones. (B) Comparison of average CNV to gene expression changes (resistant vs nonresistant cells). Top panel shows CNV log2 fold change per gene across the genome. Bottom panel shows log2 fold change in RNA expression between resistant and nonresistant clones (without mitotane treatment). Selected genes with consistent log2 fold changes (|CNV| > 0.5, |RNA| > 1, CNV*RNA > 0) are highlighted. Orange lines indicate smooth moving average. The overall correlation is 0.28. Chr, chromosome.
    Figure Legend Snippet: (A) Copy number changes compared to founder cell line. Whole-exome sequencing data of resistant and nonresistant cell lines were compared to the founder cell line in a tumor/normal matched pair fashion to analyze copy number changes. Log2 fold changes are shown across the genome. Resistant cell lines share CNV patterns that are distinct from the control, suggesting a common cell of origin of resistant clones. (B) Comparison of average CNV to gene expression changes (resistant vs nonresistant cells). Top panel shows CNV log2 fold change per gene across the genome. Bottom panel shows log2 fold change in RNA expression between resistant and nonresistant clones (without mitotane treatment). Selected genes with consistent log2 fold changes (|CNV| > 0.5, |RNA| > 1, CNV*RNA > 0) are highlighted. Orange lines indicate smooth moving average. The overall correlation is 0.28. Chr, chromosome.

    Techniques Used: Sequencing, Expressing, RNA Expression, Clone Assay

    6) Product Images from "Global Transcriptomic Profiling Using Small Volumes of Whole Blood: A Cost-Effective Method for Translational Genomic Biomarker Identification in Small Animals"

    Article Title: Global Transcriptomic Profiling Using Small Volumes of Whole Blood: A Cost-Effective Method for Translational Genomic Biomarker Identification in Small Animals

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms12042502

    Flow diagram of the study design. Varying volumes of rat whole blood (25–500 μL) were collected and immediately lysed in QIAzol (25–200 μL of blood in 1 mL QIAzol, 500 μL in 2 mL QIAzol) from rats ( n = 3 per time point per treatment) treated with either saline or LPS. Blood (2.5 mL) was also collected from each rat into PAXgene tubes. Automated RNA isolation for blood collected directly into QIAzol was performed using the Qiagen 3000 BioRobot RNeasy 96 protocol. RNA isolation for PAXgene tubes was performed manually according to manufacturer’s instructions.
    Figure Legend Snippet: Flow diagram of the study design. Varying volumes of rat whole blood (25–500 μL) were collected and immediately lysed in QIAzol (25–200 μL of blood in 1 mL QIAzol, 500 μL in 2 mL QIAzol) from rats ( n = 3 per time point per treatment) treated with either saline or LPS. Blood (2.5 mL) was also collected from each rat into PAXgene tubes. Automated RNA isolation for blood collected directly into QIAzol was performed using the Qiagen 3000 BioRobot RNeasy 96 protocol. RNA isolation for PAXgene tubes was performed manually according to manufacturer’s instructions.

    Techniques Used: Flow Cytometry, Isolation

    7) Product Images from "Transcriptional Pathways in cPGI2-Induced Adipocyte Progenitor Activation for Browning"

    Article Title: Transcriptional Pathways in cPGI2-Induced Adipocyte Progenitor Activation for Browning

    Journal: Frontiers in Endocrinology

    doi: 10.3389/fendo.2015.00129

    Synergism of cPGI 2 signaling during progenitor activation and adipocyte maturation for the induction of thermogenic marker genes . Lin − CD29 + CD34 + Sca-1 + cells were cultured in adipogenic media for 8 days. cPGI 2 was included in the media as indicated by the gray bars. Three hours before harvest, cells were cultured ± NE. RNA expression of Ucp1 (A) and Cidea (B) was analyzed by qRT-PCR [* and # indicate Tukey p
    Figure Legend Snippet: Synergism of cPGI 2 signaling during progenitor activation and adipocyte maturation for the induction of thermogenic marker genes . Lin − CD29 + CD34 + Sca-1 + cells were cultured in adipogenic media for 8 days. cPGI 2 was included in the media as indicated by the gray bars. Three hours before harvest, cells were cultured ± NE. RNA expression of Ucp1 (A) and Cidea (B) was analyzed by qRT-PCR [* and # indicate Tukey p

    Techniques Used: Activation Assay, Marker, Cell Culture, RNA Expression, Quantitative RT-PCR

    A MACS selection procedure for the prospective isolation of progenitor cells for beige/brite differentiation . (A–F) Single cell suspensions were obtained by collagenase digestion of subcutaneous fat, and stained for six-color FACS with the indicated antibodies after removal of adipocytes by centrifugation and erythrocytes by TER119-MACS ® depletion. Debris and singlets were excluded and selected through FSC/SSC and FSC-A/H, respectively. (A–C) and (D–F) represent independent gating schemes. Values (%) indicate cells % of parent plot. Representative plots from multiple independent experiments are shown. (G) Erythrocytes, leukocytes, and endothelial cells were removed from single cell suspensions in a first MACS step with biotinylated Ter119, CD45, and CD31 antibodies and streptavidin-conjugated microbeads. Sca-1 + cells were enriched in a second MACS step with Sca-1-PE-Cy7 antibody and anti-PE-Cy7 microbeads. The resulting cell population (Lin − Sca-1 + eluate) as well as the Lin − flow-through were subjected to flow cytometry. Comparable purities were obtained with directly conjugated antibody-bead combinations (data not shown). (H) MACS- and FACS-purified cells were cultured and differentiated for 8 days in the presence or absence of cPGI 2 (see Materials and Methods ) before subjected to RNA expression analysis by qRT-PCR (* indicates t -test p
    Figure Legend Snippet: A MACS selection procedure for the prospective isolation of progenitor cells for beige/brite differentiation . (A–F) Single cell suspensions were obtained by collagenase digestion of subcutaneous fat, and stained for six-color FACS with the indicated antibodies after removal of adipocytes by centrifugation and erythrocytes by TER119-MACS ® depletion. Debris and singlets were excluded and selected through FSC/SSC and FSC-A/H, respectively. (A–C) and (D–F) represent independent gating schemes. Values (%) indicate cells % of parent plot. Representative plots from multiple independent experiments are shown. (G) Erythrocytes, leukocytes, and endothelial cells were removed from single cell suspensions in a first MACS step with biotinylated Ter119, CD45, and CD31 antibodies and streptavidin-conjugated microbeads. Sca-1 + cells were enriched in a second MACS step with Sca-1-PE-Cy7 antibody and anti-PE-Cy7 microbeads. The resulting cell population (Lin − Sca-1 + eluate) as well as the Lin − flow-through were subjected to flow cytometry. Comparable purities were obtained with directly conjugated antibody-bead combinations (data not shown). (H) MACS- and FACS-purified cells were cultured and differentiated for 8 days in the presence or absence of cPGI 2 (see Materials and Methods ) before subjected to RNA expression analysis by qRT-PCR (* indicates t -test p

    Techniques Used: Magnetic Cell Separation, Selection, Isolation, Staining, FACS, Centrifugation, Flow Cytometry, Cytometry, Purification, Cell Culture, RNA Expression, Quantitative RT-PCR

    8) Product Images from "Temporal Progression of Alzheimer’s disease in Brains and Intestines of Transgenic Mice"

    Article Title: Temporal Progression of Alzheimer’s disease in Brains and Intestines of Transgenic Mice

    Journal: Neurobiology of aging

    doi: 10.1016/j.neurobiolaging.2019.05.025

    APP/PS1 and App NL-G-F colons had elevated cytokine mRNA levels in the colons of male mice. Flash frozen colons from WT, APP/PS1 and App NL-G-F female ( A ) and male ( B ) mice were lysed in QIAzol and RNA isolated. Real time PCR for TNF-α, IL-6 and IL-1β from samples were performed and fold change in mRNA expression were calculated as 2^-ΔΔCt, averaged and plotted ±SEM (n=5). Two-way ANOVA multiple comparisons indicate F 4,36 =4.703 and p=0.0037 (female, TNF-α), F 4,34 =2.573, p=0.0553 (female, IL-6), F 4,36 =1.108, p=0.3679 (female, IL-1 β), F 4,34 =2.018, p=0.1139 (male, TNF-α), F 4,35 =1.044, p=0.3987 (male, IL-6), and F 4,36 =2.058, p=0.1068 (male, IL-1β) between all interactions of age and strain. Multiple comparisons correction was determined by the Holm-Sidak method, *p
    Figure Legend Snippet: APP/PS1 and App NL-G-F colons had elevated cytokine mRNA levels in the colons of male mice. Flash frozen colons from WT, APP/PS1 and App NL-G-F female ( A ) and male ( B ) mice were lysed in QIAzol and RNA isolated. Real time PCR for TNF-α, IL-6 and IL-1β from samples were performed and fold change in mRNA expression were calculated as 2^-ΔΔCt, averaged and plotted ±SEM (n=5). Two-way ANOVA multiple comparisons indicate F 4,36 =4.703 and p=0.0037 (female, TNF-α), F 4,34 =2.573, p=0.0553 (female, IL-6), F 4,36 =1.108, p=0.3679 (female, IL-1 β), F 4,34 =2.018, p=0.1139 (male, TNF-α), F 4,35 =1.044, p=0.3987 (male, IL-6), and F 4,36 =2.058, p=0.1068 (male, IL-1β) between all interactions of age and strain. Multiple comparisons correction was determined by the Holm-Sidak method, *p

    Techniques Used: Mouse Assay, Isolation, Real-time Polymerase Chain Reaction, Expressing

    APP/PS1 and App NL-G-F had elevated cytokine mRNA levels in temporal cortices compared to WT controls. Temporal cortices from WT, APP/PS1 and App NL-G-F female ( A ) and male ( B ) mice were lysed in QIAzol and RNA isolated. Real time PCR for TNF-α, IL-6 and IL-1β from samples were performed and fold change in mRNA expression were calculated as 2^-ΔΔCt, averaged and plotted ±SEM (n=5). Two-way ANOVA multiple comparisons indicate F 4,30 =2.484 and p=0.064 (female, TNF-α), F 4,31 =3.37, p=0.0211 (female, IL-6), F 4,31 =2.113, p=0.1029 (female, IL-1β), F 4,34 =3.446, p=0.0181 (male, TNF-α), F 4,34 =1.507, p=0.222 (male, IL-6), and F 4,34 =4.476, p=0.005 (male, IL-1β) between all interactions of age and strain. Multiple comparisons correction was determined by the Holm-Sidak method, *p
    Figure Legend Snippet: APP/PS1 and App NL-G-F had elevated cytokine mRNA levels in temporal cortices compared to WT controls. Temporal cortices from WT, APP/PS1 and App NL-G-F female ( A ) and male ( B ) mice were lysed in QIAzol and RNA isolated. Real time PCR for TNF-α, IL-6 and IL-1β from samples were performed and fold change in mRNA expression were calculated as 2^-ΔΔCt, averaged and plotted ±SEM (n=5). Two-way ANOVA multiple comparisons indicate F 4,30 =2.484 and p=0.064 (female, TNF-α), F 4,31 =3.37, p=0.0211 (female, IL-6), F 4,31 =2.113, p=0.1029 (female, IL-1β), F 4,34 =3.446, p=0.0181 (male, TNF-α), F 4,34 =1.507, p=0.222 (male, IL-6), and F 4,34 =4.476, p=0.005 (male, IL-1β) between all interactions of age and strain. Multiple comparisons correction was determined by the Holm-Sidak method, *p

    Techniques Used: Mouse Assay, Isolation, Real-time Polymerase Chain Reaction, Expressing

    Related Articles

    Quantitative RT-PCR:

    Article Title: Dendrobium moniliforme Exerts Inhibitory Effects on Both Receptor Activator of Nuclear Factor Kappa-B Ligand-Mediated Osteoclast Differentiation in Vitro and Lipopolysaccharide-Induced Bone Erosion in Vivo
    Article Snippet: After the membrane was treated with primary and secondary antibodies (horseradish peroxidase [HRP]-conjugated sheep anti-mouse or donkey anti-rabbit immunoglobulin), the expression of specific protein signals was measured using a chemiluminescence detection system (Millipore). .. Quantitative Real-Time RT-PCR Analysis Total RNA was extracted using the QIAzol lysis reagent (Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions. .. Equal amounts of cDNA were synthesized from 1 μg of total RNA using SuperScript II Reverse Transcriptase (Invitrogen, San Diego, CA, USA).

    Lysis:

    Article Title: Dendrobium moniliforme Exerts Inhibitory Effects on Both Receptor Activator of Nuclear Factor Kappa-B Ligand-Mediated Osteoclast Differentiation in Vitro and Lipopolysaccharide-Induced Bone Erosion in Vivo
    Article Snippet: After the membrane was treated with primary and secondary antibodies (horseradish peroxidase [HRP]-conjugated sheep anti-mouse or donkey anti-rabbit immunoglobulin), the expression of specific protein signals was measured using a chemiluminescence detection system (Millipore). .. Quantitative Real-Time RT-PCR Analysis Total RNA was extracted using the QIAzol lysis reagent (Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions. .. Equal amounts of cDNA were synthesized from 1 μg of total RNA using SuperScript II Reverse Transcriptase (Invitrogen, San Diego, CA, USA).

    Article Title: Dual Effect of Chrysanthemum indicum Extract to Stimulate Osteoblast Differentiation and Inhibit Osteoclast Formation and Resorption In Vitro
    Article Snippet: After the membrane was treated with primary and secondary antibodies (horseradish peroxidase-conjugated sheep anti-mouse or donkey anti-rabbit immunoglobulin), expression of specific protein signals was measured using a chemiluminescence detection system (Millipore). .. Quantitative Real-Time Polymerase Chain Reaction (PCR) Analysis Total RNA was extracted using QIAzol lysis reagent (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions, and equal amounts of cDNA from the RNA samples were synthesized using 1 μ g of total RNA and SuperScript II Reverse Transcriptase (Invitrogen, San Diego, CA, USA). .. Real-time PCR was performed using an Exicycler 96 Real-Time Quantitative Thermal Block (Bioneer Co., Daejeon, Korea) with a 20 μ L reaction mixture containing 10 μ L SYBR Green Premix (Bioneer Co.), 10 pmol forward primer, 10 pmol reverse primer, and 1 μ g cDNA.

    Article Title: Aconitum pseudo-laeve var. erectum Inhibits Receptor Activator of Nuclear Factor Kappa-B Ligand-Induced Osteoclastogenesis via the c-Fos/nuclear Factor of Activated T-Cells, Cytoplasmic 1 Signaling Pathway and Prevents Lipopolysaccharide-Induced Bone Loss in Mice
    Article Snippet: After the membrane was treated with primary and secondary antibodies (horseradish peroxidase [HRP]-conjugated sheep anti-mouse or donkey anti-rabbit immunoglobulin), the expression of specific protein signals was measured using a chemiluminescence detection system (Millipore). .. Quantitative Real-time PCR Analysis Total RNA was extracted using QIAzol lysis reagent (Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions and equal amounts of the cDNA of RNA were synthesized from 1 µg of total RNA using SuperScript II Reverse Transcriptase (Invitrogen, San Diego, CA, USA). .. Real-time PCR was performed using a Exicycler™ 96 Real-Time Quantitative Thermal Block (Bioneer Co., Daejeon, Korea) in a 20 µL reaction mixture containing 10 µL SYBR Green Premix (Bioneer Co.), 10 pmol forward primer, 10 pmol reverse primer, and 1 µg cDNA.

    Article Title: The methyltransferase Setdb2 mediates virus-induced susceptibility to bacterial superinfection
    Article Snippet: .. Real-time PCR For the measurement of gene expression by real-time PCR, total RNA was isolated using QIAzol lysis reagent (Qiagen) and reverse-transcribed with the First Strand cDNA Synthesis Kit (Fermentas). .. Subsequently gene expression was analyzed using Taqman Fast Universal PCR Mastermix and Taqman Gene Expression assays (Setdb2 : Mm01318748_m1, Cxcl1 : Mm00433859_m1, Il6 : Mm00446190_m1) (Life Technologies) as well as an assay for the M gene of influenza virus A/PR/8/34 using the oligonucleotides F 5’- CATGGAATGGCTAAAGACAAGACC-3’, R 5’- CCATTAAGGGCATTTTGGACA-3` and taqman probe 5’-FAM- TTTGTGTTCACGCTCACCGTGCCCA-BHQ1-3’.

    Article Title: Nutritional Conditions Modulate C. neoformans Extracellular Vesicles’ Capacity to Elicit Host Immune Response
    Article Snippet: .. RNA Extraction and Reverse-Transcription Polymerase Chain Reaction (RT-PCR) For RNA extraction, lung fragments were macerated using 1 mL of the QIAzol Lysis Reagent buffer (Qiagen—Hilden, Germany). .. The subsequent steps were done using the RNeasy mini-kit (Qiagen), following the manufacturer’s instructions.

    Real-time Polymerase Chain Reaction:

    Article Title: Dual Effect of Chrysanthemum indicum Extract to Stimulate Osteoblast Differentiation and Inhibit Osteoclast Formation and Resorption In Vitro
    Article Snippet: After the membrane was treated with primary and secondary antibodies (horseradish peroxidase-conjugated sheep anti-mouse or donkey anti-rabbit immunoglobulin), expression of specific protein signals was measured using a chemiluminescence detection system (Millipore). .. Quantitative Real-Time Polymerase Chain Reaction (PCR) Analysis Total RNA was extracted using QIAzol lysis reagent (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions, and equal amounts of cDNA from the RNA samples were synthesized using 1 μ g of total RNA and SuperScript II Reverse Transcriptase (Invitrogen, San Diego, CA, USA). .. Real-time PCR was performed using an Exicycler 96 Real-Time Quantitative Thermal Block (Bioneer Co., Daejeon, Korea) with a 20 μ L reaction mixture containing 10 μ L SYBR Green Premix (Bioneer Co.), 10 pmol forward primer, 10 pmol reverse primer, and 1 μ g cDNA.

    Article Title: Aconitum pseudo-laeve var. erectum Inhibits Receptor Activator of Nuclear Factor Kappa-B Ligand-Induced Osteoclastogenesis via the c-Fos/nuclear Factor of Activated T-Cells, Cytoplasmic 1 Signaling Pathway and Prevents Lipopolysaccharide-Induced Bone Loss in Mice
    Article Snippet: After the membrane was treated with primary and secondary antibodies (horseradish peroxidase [HRP]-conjugated sheep anti-mouse or donkey anti-rabbit immunoglobulin), the expression of specific protein signals was measured using a chemiluminescence detection system (Millipore). .. Quantitative Real-time PCR Analysis Total RNA was extracted using QIAzol lysis reagent (Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions and equal amounts of the cDNA of RNA were synthesized from 1 µg of total RNA using SuperScript II Reverse Transcriptase (Invitrogen, San Diego, CA, USA). .. Real-time PCR was performed using a Exicycler™ 96 Real-Time Quantitative Thermal Block (Bioneer Co., Daejeon, Korea) in a 20 µL reaction mixture containing 10 µL SYBR Green Premix (Bioneer Co.), 10 pmol forward primer, 10 pmol reverse primer, and 1 µg cDNA.

    Article Title: The methyltransferase Setdb2 mediates virus-induced susceptibility to bacterial superinfection
    Article Snippet: .. Real-time PCR For the measurement of gene expression by real-time PCR, total RNA was isolated using QIAzol lysis reagent (Qiagen) and reverse-transcribed with the First Strand cDNA Synthesis Kit (Fermentas). .. Subsequently gene expression was analyzed using Taqman Fast Universal PCR Mastermix and Taqman Gene Expression assays (Setdb2 : Mm01318748_m1, Cxcl1 : Mm00433859_m1, Il6 : Mm00446190_m1) (Life Technologies) as well as an assay for the M gene of influenza virus A/PR/8/34 using the oligonucleotides F 5’- CATGGAATGGCTAAAGACAAGACC-3’, R 5’- CCATTAAGGGCATTTTGGACA-3` and taqman probe 5’-FAM- TTTGTGTTCACGCTCACCGTGCCCA-BHQ1-3’.

    Polymerase Chain Reaction:

    Article Title: Dual Effect of Chrysanthemum indicum Extract to Stimulate Osteoblast Differentiation and Inhibit Osteoclast Formation and Resorption In Vitro
    Article Snippet: After the membrane was treated with primary and secondary antibodies (horseradish peroxidase-conjugated sheep anti-mouse or donkey anti-rabbit immunoglobulin), expression of specific protein signals was measured using a chemiluminescence detection system (Millipore). .. Quantitative Real-Time Polymerase Chain Reaction (PCR) Analysis Total RNA was extracted using QIAzol lysis reagent (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions, and equal amounts of cDNA from the RNA samples were synthesized using 1 μ g of total RNA and SuperScript II Reverse Transcriptase (Invitrogen, San Diego, CA, USA). .. Real-time PCR was performed using an Exicycler 96 Real-Time Quantitative Thermal Block (Bioneer Co., Daejeon, Korea) with a 20 μ L reaction mixture containing 10 μ L SYBR Green Premix (Bioneer Co.), 10 pmol forward primer, 10 pmol reverse primer, and 1 μ g cDNA.

    Synthesized:

    Article Title: Dual Effect of Chrysanthemum indicum Extract to Stimulate Osteoblast Differentiation and Inhibit Osteoclast Formation and Resorption In Vitro
    Article Snippet: After the membrane was treated with primary and secondary antibodies (horseradish peroxidase-conjugated sheep anti-mouse or donkey anti-rabbit immunoglobulin), expression of specific protein signals was measured using a chemiluminescence detection system (Millipore). .. Quantitative Real-Time Polymerase Chain Reaction (PCR) Analysis Total RNA was extracted using QIAzol lysis reagent (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions, and equal amounts of cDNA from the RNA samples were synthesized using 1 μ g of total RNA and SuperScript II Reverse Transcriptase (Invitrogen, San Diego, CA, USA). .. Real-time PCR was performed using an Exicycler 96 Real-Time Quantitative Thermal Block (Bioneer Co., Daejeon, Korea) with a 20 μ L reaction mixture containing 10 μ L SYBR Green Premix (Bioneer Co.), 10 pmol forward primer, 10 pmol reverse primer, and 1 μ g cDNA.

    Article Title: Aconitum pseudo-laeve var. erectum Inhibits Receptor Activator of Nuclear Factor Kappa-B Ligand-Induced Osteoclastogenesis via the c-Fos/nuclear Factor of Activated T-Cells, Cytoplasmic 1 Signaling Pathway and Prevents Lipopolysaccharide-Induced Bone Loss in Mice
    Article Snippet: After the membrane was treated with primary and secondary antibodies (horseradish peroxidase [HRP]-conjugated sheep anti-mouse or donkey anti-rabbit immunoglobulin), the expression of specific protein signals was measured using a chemiluminescence detection system (Millipore). .. Quantitative Real-time PCR Analysis Total RNA was extracted using QIAzol lysis reagent (Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions and equal amounts of the cDNA of RNA were synthesized from 1 µg of total RNA using SuperScript II Reverse Transcriptase (Invitrogen, San Diego, CA, USA). .. Real-time PCR was performed using a Exicycler™ 96 Real-Time Quantitative Thermal Block (Bioneer Co., Daejeon, Korea) in a 20 µL reaction mixture containing 10 µL SYBR Green Premix (Bioneer Co.), 10 pmol forward primer, 10 pmol reverse primer, and 1 µg cDNA.

    Isolation:

    Article Title: Temporal Progression of Alzheimer’s disease in Brains and Intestines of Transgenic Mice
    Article Snippet: The Aβ 1-40 and Aβ 1-42 ELISA kits were obtained from EMD Millipore (Burlington, MA). .. QIAzol and RNeasy mini kit for RNA isolation were purchased from Qiagen (Germantown, MA) and iTaq Universal SYBR Green One-Step kit was from Biorad (Hercules, CA). .. FITC-dextran was purchased from Sigma Aldrich (St. Louis, MO).

    Article Title: The methyltransferase Setdb2 mediates virus-induced susceptibility to bacterial superinfection
    Article Snippet: .. Real-time PCR For the measurement of gene expression by real-time PCR, total RNA was isolated using QIAzol lysis reagent (Qiagen) and reverse-transcribed with the First Strand cDNA Synthesis Kit (Fermentas). .. Subsequently gene expression was analyzed using Taqman Fast Universal PCR Mastermix and Taqman Gene Expression assays (Setdb2 : Mm01318748_m1, Cxcl1 : Mm00433859_m1, Il6 : Mm00446190_m1) (Life Technologies) as well as an assay for the M gene of influenza virus A/PR/8/34 using the oligonucleotides F 5’- CATGGAATGGCTAAAGACAAGACC-3’, R 5’- CCATTAAGGGCATTTTGGACA-3` and taqman probe 5’-FAM- TTTGTGTTCACGCTCACCGTGCCCA-BHQ1-3’.

    Article Title: Controlling technical variation amongst 6693 patient microarrays of the randomized MINDACT trial
    Article Snippet: Sample preparation and microarray hybridization RNA was isolated from a patient’s fresh frozen tumor sample and was amplified as described previously . .. As shown in Fig. , one of the FDA/QSR-compliant technical changes involved the use of two different batches of RNA-Bee isolation reagent (Tel Test; RNA-Bee batch 1 and RNA-Bee batch 2) and one batch of Qiazol (Qiagen). .. A manufacturer’s change in the RNA-extraction solution RNA-Bee (that was not communicated by the manufacturer) caused a temporary shift in the MammaPrint risk calculation (for more information see Cardoso et al. ) from May 24, 2009, to January 30, 2010, at which time the issue rectified with the use of a new reference RNA (see below).

    SYBR Green Assay:

    Article Title: Temporal Progression of Alzheimer’s disease in Brains and Intestines of Transgenic Mice
    Article Snippet: The Aβ 1-40 and Aβ 1-42 ELISA kits were obtained from EMD Millipore (Burlington, MA). .. QIAzol and RNeasy mini kit for RNA isolation were purchased from Qiagen (Germantown, MA) and iTaq Universal SYBR Green One-Step kit was from Biorad (Hercules, CA). .. FITC-dextran was purchased from Sigma Aldrich (St. Louis, MO).

    Expressing:

    Article Title: The methyltransferase Setdb2 mediates virus-induced susceptibility to bacterial superinfection
    Article Snippet: .. Real-time PCR For the measurement of gene expression by real-time PCR, total RNA was isolated using QIAzol lysis reagent (Qiagen) and reverse-transcribed with the First Strand cDNA Synthesis Kit (Fermentas). .. Subsequently gene expression was analyzed using Taqman Fast Universal PCR Mastermix and Taqman Gene Expression assays (Setdb2 : Mm01318748_m1, Cxcl1 : Mm00433859_m1, Il6 : Mm00446190_m1) (Life Technologies) as well as an assay for the M gene of influenza virus A/PR/8/34 using the oligonucleotides F 5’- CATGGAATGGCTAAAGACAAGACC-3’, R 5’- CCATTAAGGGCATTTTGGACA-3` and taqman probe 5’-FAM- TTTGTGTTCACGCTCACCGTGCCCA-BHQ1-3’.

    RNA Extraction:

    Article Title: Nutritional Conditions Modulate C. neoformans Extracellular Vesicles’ Capacity to Elicit Host Immune Response
    Article Snippet: .. RNA Extraction and Reverse-Transcription Polymerase Chain Reaction (RT-PCR) For RNA extraction, lung fragments were macerated using 1 mL of the QIAzol Lysis Reagent buffer (Qiagen—Hilden, Germany). .. The subsequent steps were done using the RNeasy mini-kit (Qiagen), following the manufacturer’s instructions.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Nutritional Conditions Modulate C. neoformans Extracellular Vesicles’ Capacity to Elicit Host Immune Response
    Article Snippet: .. RNA Extraction and Reverse-Transcription Polymerase Chain Reaction (RT-PCR) For RNA extraction, lung fragments were macerated using 1 mL of the QIAzol Lysis Reagent buffer (Qiagen—Hilden, Germany). .. The subsequent steps were done using the RNeasy mini-kit (Qiagen), following the manufacturer’s instructions.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    Qiagen qiazol lysis reagent
    DM suppresses the expressions of c-Fos, NFATc1, and other osteoclast marker genes. ( A ) BMMs were stimulated with RANKL (50 ng/mL) and M-CSF (30 ng/mL) in the presence or absence of DM (50 ng/mL) for the indicated times. Total RNA was isolated from cells using <t>QIAzol</t> reagent; mRNA expression levels of c-Fos and NFATc1 were evaluated using quantitative real-time <t>RT-PCR.</t> *** p
    Qiazol Lysis Reagent, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiazol lysis reagent/product/Qiagen
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    qiazol lysis reagent - by Bioz Stars, 2021-03
    97/100 stars
      Buy from Supplier

    Image Search Results


    DM suppresses the expressions of c-Fos, NFATc1, and other osteoclast marker genes. ( A ) BMMs were stimulated with RANKL (50 ng/mL) and M-CSF (30 ng/mL) in the presence or absence of DM (50 ng/mL) for the indicated times. Total RNA was isolated from cells using QIAzol reagent; mRNA expression levels of c-Fos and NFATc1 were evaluated using quantitative real-time RT-PCR. *** p

    Journal: Molecules

    Article Title: Dendrobium moniliforme Exerts Inhibitory Effects on Both Receptor Activator of Nuclear Factor Kappa-B Ligand-Mediated Osteoclast Differentiation in Vitro and Lipopolysaccharide-Induced Bone Erosion in Vivo

    doi: 10.3390/molecules21030295

    Figure Lengend Snippet: DM suppresses the expressions of c-Fos, NFATc1, and other osteoclast marker genes. ( A ) BMMs were stimulated with RANKL (50 ng/mL) and M-CSF (30 ng/mL) in the presence or absence of DM (50 ng/mL) for the indicated times. Total RNA was isolated from cells using QIAzol reagent; mRNA expression levels of c-Fos and NFATc1 were evaluated using quantitative real-time RT-PCR. *** p

    Article Snippet: Quantitative Real-Time RT-PCR Analysis Total RNA was extracted using the QIAzol lysis reagent (Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions.

    Techniques: Marker, Isolation, Expressing, Quantitative RT-PCR

    CIE downregulates RANKL-induced early signals and marker genes during osteoclastogenesis. (a) BMMs were pretreated with DMSO (control) or CIE (50 μ g/mL) for 1 h in the presence of M-CSF (30 ng/mL) and were stimulated with RANKL (100 ng/mL) for the indicated times. Whole-cell lysates were used for western blot analysis with the specified antibodies. β -Actin served as the internal control. (b) BMMs were stimulated with RANKL (100 ng/mL) and M-CSF (30 ng/mL) in the presence or absence of CIE (50 μ g/mL) for the indicated times. Total RNA was isolated from cells using QIAzol reagent and the mRNA expression levels of OSCAR, TRAP, integrin α v, β 3, DC-STAMP, OC-STAMP, cathepsin K, and ICAM-1 were evaluated by real-time PCR. * P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Dual Effect of Chrysanthemum indicum Extract to Stimulate Osteoblast Differentiation and Inhibit Osteoclast Formation and Resorption In Vitro

    doi: 10.1155/2014/176049

    Figure Lengend Snippet: CIE downregulates RANKL-induced early signals and marker genes during osteoclastogenesis. (a) BMMs were pretreated with DMSO (control) or CIE (50 μ g/mL) for 1 h in the presence of M-CSF (30 ng/mL) and were stimulated with RANKL (100 ng/mL) for the indicated times. Whole-cell lysates were used for western blot analysis with the specified antibodies. β -Actin served as the internal control. (b) BMMs were stimulated with RANKL (100 ng/mL) and M-CSF (30 ng/mL) in the presence or absence of CIE (50 μ g/mL) for the indicated times. Total RNA was isolated from cells using QIAzol reagent and the mRNA expression levels of OSCAR, TRAP, integrin α v, β 3, DC-STAMP, OC-STAMP, cathepsin K, and ICAM-1 were evaluated by real-time PCR. * P

    Article Snippet: Quantitative Real-Time Polymerase Chain Reaction (PCR) Analysis Total RNA was extracted using QIAzol lysis reagent (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions, and equal amounts of cDNA from the RNA samples were synthesized using 1 μ g of total RNA and SuperScript II Reverse Transcriptase (Invitrogen, San Diego, CA, USA).

    Techniques: Marker, Western Blot, Isolation, Expressing, Real-time Polymerase Chain Reaction

    CIE promotes ascorbic acid and β -glycerol phosphate mediated osteoblast differentiation. (a) Primary osteoblasts were treated with various concentrations of CIE for 7 days in the presence of 50 μ g/mL ascorbic acid and 10 mM β -glycerol phosphate. ALP-positive cells were stained with ALP solution. (b) Primary osteoblasts were treated with various concentrations of CIE for 21 days. Calcium accumulation within the osteoblasts was stained with ARS solution. Stained calcium deposits were dissolved by 10% CPC buffer to measure the level of staining. (c) Primary osteoblasts were stimulated with ascorbic acid (50 μ g/mL) and β -glycerol phosphate in the presence of CIE (50 μ g/mL) or DMSO. Total RNA was isolated from cells using QIAzol reagent and mRNA expression levels of Runx2, ALP, Col1 α , and OPN were evaluated by real-time PCR. * P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Dual Effect of Chrysanthemum indicum Extract to Stimulate Osteoblast Differentiation and Inhibit Osteoclast Formation and Resorption In Vitro

    doi: 10.1155/2014/176049

    Figure Lengend Snippet: CIE promotes ascorbic acid and β -glycerol phosphate mediated osteoblast differentiation. (a) Primary osteoblasts were treated with various concentrations of CIE for 7 days in the presence of 50 μ g/mL ascorbic acid and 10 mM β -glycerol phosphate. ALP-positive cells were stained with ALP solution. (b) Primary osteoblasts were treated with various concentrations of CIE for 21 days. Calcium accumulation within the osteoblasts was stained with ARS solution. Stained calcium deposits were dissolved by 10% CPC buffer to measure the level of staining. (c) Primary osteoblasts were stimulated with ascorbic acid (50 μ g/mL) and β -glycerol phosphate in the presence of CIE (50 μ g/mL) or DMSO. Total RNA was isolated from cells using QIAzol reagent and mRNA expression levels of Runx2, ALP, Col1 α , and OPN were evaluated by real-time PCR. * P

    Article Snippet: Quantitative Real-Time Polymerase Chain Reaction (PCR) Analysis Total RNA was extracted using QIAzol lysis reagent (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions, and equal amounts of cDNA from the RNA samples were synthesized using 1 μ g of total RNA and SuperScript II Reverse Transcriptase (Invitrogen, San Diego, CA, USA).

    Techniques: ALP Assay, Staining, Isolation, Expressing, Real-time Polymerase Chain Reaction

    CIE suppresses RANKL-induced c-Fos and NFATc1 expression. (a) Effects of CIE on levels of c-Fos and NFATc1 protein expression were evaluated using western blot analysis. β -Actin was used as the internal control. (b) BMMs were stimulated with RANKL (100 ng/mL) and M-CSF (30 ng/mL) in the presence or absence of CIE (50 μ g/mL) for the specified times. Total RNA was isolated from cells using QIAzol reagent and mRNA expression levels of c-Fos and NFATc1 were evaluated using real-time PCR. * P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Dual Effect of Chrysanthemum indicum Extract to Stimulate Osteoblast Differentiation and Inhibit Osteoclast Formation and Resorption In Vitro

    doi: 10.1155/2014/176049

    Figure Lengend Snippet: CIE suppresses RANKL-induced c-Fos and NFATc1 expression. (a) Effects of CIE on levels of c-Fos and NFATc1 protein expression were evaluated using western blot analysis. β -Actin was used as the internal control. (b) BMMs were stimulated with RANKL (100 ng/mL) and M-CSF (30 ng/mL) in the presence or absence of CIE (50 μ g/mL) for the specified times. Total RNA was isolated from cells using QIAzol reagent and mRNA expression levels of c-Fos and NFATc1 were evaluated using real-time PCR. * P

    Article Snippet: Quantitative Real-Time Polymerase Chain Reaction (PCR) Analysis Total RNA was extracted using QIAzol lysis reagent (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions, and equal amounts of cDNA from the RNA samples were synthesized using 1 μ g of total RNA and SuperScript II Reverse Transcriptase (Invitrogen, San Diego, CA, USA).

    Techniques: Expressing, Western Blot, Isolation, Real-time Polymerase Chain Reaction

    APP/PS1 and App NL-G-F colons had elevated cytokine mRNA levels in the colons of male mice. Flash frozen colons from WT, APP/PS1 and App NL-G-F female ( A ) and male ( B ) mice were lysed in QIAzol and RNA isolated. Real time PCR for TNF-α, IL-6 and IL-1β from samples were performed and fold change in mRNA expression were calculated as 2^-ΔΔCt, averaged and plotted ±SEM (n=5). Two-way ANOVA multiple comparisons indicate F 4,36 =4.703 and p=0.0037 (female, TNF-α), F 4,34 =2.573, p=0.0553 (female, IL-6), F 4,36 =1.108, p=0.3679 (female, IL-1 β), F 4,34 =2.018, p=0.1139 (male, TNF-α), F 4,35 =1.044, p=0.3987 (male, IL-6), and F 4,36 =2.058, p=0.1068 (male, IL-1β) between all interactions of age and strain. Multiple comparisons correction was determined by the Holm-Sidak method, *p

    Journal: Neurobiology of aging

    Article Title: Temporal Progression of Alzheimer’s disease in Brains and Intestines of Transgenic Mice

    doi: 10.1016/j.neurobiolaging.2019.05.025

    Figure Lengend Snippet: APP/PS1 and App NL-G-F colons had elevated cytokine mRNA levels in the colons of male mice. Flash frozen colons from WT, APP/PS1 and App NL-G-F female ( A ) and male ( B ) mice were lysed in QIAzol and RNA isolated. Real time PCR for TNF-α, IL-6 and IL-1β from samples were performed and fold change in mRNA expression were calculated as 2^-ΔΔCt, averaged and plotted ±SEM (n=5). Two-way ANOVA multiple comparisons indicate F 4,36 =4.703 and p=0.0037 (female, TNF-α), F 4,34 =2.573, p=0.0553 (female, IL-6), F 4,36 =1.108, p=0.3679 (female, IL-1 β), F 4,34 =2.018, p=0.1139 (male, TNF-α), F 4,35 =1.044, p=0.3987 (male, IL-6), and F 4,36 =2.058, p=0.1068 (male, IL-1β) between all interactions of age and strain. Multiple comparisons correction was determined by the Holm-Sidak method, *p

    Article Snippet: QIAzol and RNeasy mini kit for RNA isolation were purchased from Qiagen (Germantown, MA) and iTaq Universal SYBR Green One-Step kit was from Biorad (Hercules, CA).

    Techniques: Mouse Assay, Isolation, Real-time Polymerase Chain Reaction, Expressing

    APP/PS1 and App NL-G-F had elevated cytokine mRNA levels in temporal cortices compared to WT controls. Temporal cortices from WT, APP/PS1 and App NL-G-F female ( A ) and male ( B ) mice were lysed in QIAzol and RNA isolated. Real time PCR for TNF-α, IL-6 and IL-1β from samples were performed and fold change in mRNA expression were calculated as 2^-ΔΔCt, averaged and plotted ±SEM (n=5). Two-way ANOVA multiple comparisons indicate F 4,30 =2.484 and p=0.064 (female, TNF-α), F 4,31 =3.37, p=0.0211 (female, IL-6), F 4,31 =2.113, p=0.1029 (female, IL-1β), F 4,34 =3.446, p=0.0181 (male, TNF-α), F 4,34 =1.507, p=0.222 (male, IL-6), and F 4,34 =4.476, p=0.005 (male, IL-1β) between all interactions of age and strain. Multiple comparisons correction was determined by the Holm-Sidak method, *p

    Journal: Neurobiology of aging

    Article Title: Temporal Progression of Alzheimer’s disease in Brains and Intestines of Transgenic Mice

    doi: 10.1016/j.neurobiolaging.2019.05.025

    Figure Lengend Snippet: APP/PS1 and App NL-G-F had elevated cytokine mRNA levels in temporal cortices compared to WT controls. Temporal cortices from WT, APP/PS1 and App NL-G-F female ( A ) and male ( B ) mice were lysed in QIAzol and RNA isolated. Real time PCR for TNF-α, IL-6 and IL-1β from samples were performed and fold change in mRNA expression were calculated as 2^-ΔΔCt, averaged and plotted ±SEM (n=5). Two-way ANOVA multiple comparisons indicate F 4,30 =2.484 and p=0.064 (female, TNF-α), F 4,31 =3.37, p=0.0211 (female, IL-6), F 4,31 =2.113, p=0.1029 (female, IL-1β), F 4,34 =3.446, p=0.0181 (male, TNF-α), F 4,34 =1.507, p=0.222 (male, IL-6), and F 4,34 =4.476, p=0.005 (male, IL-1β) between all interactions of age and strain. Multiple comparisons correction was determined by the Holm-Sidak method, *p

    Article Snippet: QIAzol and RNeasy mini kit for RNA isolation were purchased from Qiagen (Germantown, MA) and iTaq Universal SYBR Green One-Step kit was from Biorad (Hercules, CA).

    Techniques: Mouse Assay, Isolation, Real-time Polymerase Chain Reaction, Expressing

    Aconitum pseudo-laeve var. erectum (APE) suppresses receptor activator of nuclear factor kappa-B ligand (RANKL)-induced c-Fos and nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1) expression without stimulating early signal pathways. ( A ) Bone marrow macrophages (BMMs) were pretreated with DMSO (control) or APE (200 µg/mL) for 1 h in the presence of macrophage colony-stimulating factor (M-CSF; 30 ng/mL) and were stimulated with RANKL (100 ng/mL) for the indicated times. Whole-cell lysates underwent western blot analysis with the various indicated antibodies. β-actin served as the internal control; ( B ) BMMs were stimulated with RANKL (100 ng/mL) and M-CSF (30 ng/mL) in the presence or absence of APE (200 µg/mL) for the indicated times. Total RNA was isolated from cells using QIAzol reagent and mRNA expression levels of c-Fos and NFATc1 were evaluated using real-time PCR; ( C ) Effects of APE on protein expression levels of c-Fos and NFATc1 were evaluated using western blot analysis. β-actin was used as the internal control; ( D ) BMMs were infected with retroviruses expressing pMX-IRES-EGFP (pMX), pMX-NFATc1-EGFP, and pMX-cFos-EGFP. Infected BMMs were cultured with or without APE (200 µg/mL) in the presence of M-CSF (30 ng/mL) and RANKL (100 ng/mL) for 4 days. After culturing, the cells were fixed and stained for tartrate-resistant acid phosphatase (TRAP) (left). The TRAP-positive multinucleated osteoclasts were counted (right).

    Journal: Molecules

    Article Title: Aconitum pseudo-laeve var. erectum Inhibits Receptor Activator of Nuclear Factor Kappa-B Ligand-Induced Osteoclastogenesis via the c-Fos/nuclear Factor of Activated T-Cells, Cytoplasmic 1 Signaling Pathway and Prevents Lipopolysaccharide-Induced Bone Loss in Mice

    doi: 10.3390/molecules190811628

    Figure Lengend Snippet: Aconitum pseudo-laeve var. erectum (APE) suppresses receptor activator of nuclear factor kappa-B ligand (RANKL)-induced c-Fos and nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1) expression without stimulating early signal pathways. ( A ) Bone marrow macrophages (BMMs) were pretreated with DMSO (control) or APE (200 µg/mL) for 1 h in the presence of macrophage colony-stimulating factor (M-CSF; 30 ng/mL) and were stimulated with RANKL (100 ng/mL) for the indicated times. Whole-cell lysates underwent western blot analysis with the various indicated antibodies. β-actin served as the internal control; ( B ) BMMs were stimulated with RANKL (100 ng/mL) and M-CSF (30 ng/mL) in the presence or absence of APE (200 µg/mL) for the indicated times. Total RNA was isolated from cells using QIAzol reagent and mRNA expression levels of c-Fos and NFATc1 were evaluated using real-time PCR; ( C ) Effects of APE on protein expression levels of c-Fos and NFATc1 were evaluated using western blot analysis. β-actin was used as the internal control; ( D ) BMMs were infected with retroviruses expressing pMX-IRES-EGFP (pMX), pMX-NFATc1-EGFP, and pMX-cFos-EGFP. Infected BMMs were cultured with or without APE (200 µg/mL) in the presence of M-CSF (30 ng/mL) and RANKL (100 ng/mL) for 4 days. After culturing, the cells were fixed and stained for tartrate-resistant acid phosphatase (TRAP) (left). The TRAP-positive multinucleated osteoclasts were counted (right).

    Article Snippet: Quantitative Real-time PCR Analysis Total RNA was extracted using QIAzol lysis reagent (Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions and equal amounts of the cDNA of RNA were synthesized from 1 µg of total RNA using SuperScript II Reverse Transcriptase (Invitrogen, San Diego, CA, USA).

    Techniques: Expressing, Western Blot, Isolation, Real-time Polymerase Chain Reaction, Infection, Cell Culture, Staining

    Aconitum pseudo-laeve var. erectum (APE) down-regulates the expression of osteoclast-specific marker genes. Bone marrow macrophages (BMMs) were stimulated with receptor activator of nuclear factor kappa-B ligand (RANKL; 100 ng/mL) and macrophage colony-stimulating factor (M-CSF; 30 ng/mL) in the presence or absence of APE (200 µg/mL) for the indicated times. Total RNA was isolated from cells using QIAzol reagent and mRNA expression levels of osteoclast-associated receptor (OSCAR), tartrate-resistant acid phosphatase (TRAP), Atp6v0d2, Cathepsin K, dendritic cell-specific transmembrane protein (DC-STAMP), osteoclast stimulatory transmembrane protein (OC-STAMP), calcitonin receptor, and matrix metallopeptidase 9 (MMP-9) were evaluated by real-time PCR.

    Journal: Molecules

    Article Title: Aconitum pseudo-laeve var. erectum Inhibits Receptor Activator of Nuclear Factor Kappa-B Ligand-Induced Osteoclastogenesis via the c-Fos/nuclear Factor of Activated T-Cells, Cytoplasmic 1 Signaling Pathway and Prevents Lipopolysaccharide-Induced Bone Loss in Mice

    doi: 10.3390/molecules190811628

    Figure Lengend Snippet: Aconitum pseudo-laeve var. erectum (APE) down-regulates the expression of osteoclast-specific marker genes. Bone marrow macrophages (BMMs) were stimulated with receptor activator of nuclear factor kappa-B ligand (RANKL; 100 ng/mL) and macrophage colony-stimulating factor (M-CSF; 30 ng/mL) in the presence or absence of APE (200 µg/mL) for the indicated times. Total RNA was isolated from cells using QIAzol reagent and mRNA expression levels of osteoclast-associated receptor (OSCAR), tartrate-resistant acid phosphatase (TRAP), Atp6v0d2, Cathepsin K, dendritic cell-specific transmembrane protein (DC-STAMP), osteoclast stimulatory transmembrane protein (OC-STAMP), calcitonin receptor, and matrix metallopeptidase 9 (MMP-9) were evaluated by real-time PCR.

    Article Snippet: Quantitative Real-time PCR Analysis Total RNA was extracted using QIAzol lysis reagent (Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions and equal amounts of the cDNA of RNA were synthesized from 1 µg of total RNA using SuperScript II Reverse Transcriptase (Invitrogen, San Diego, CA, USA).

    Techniques: Expressing, Marker, Isolation, Real-time Polymerase Chain Reaction