7900ht fast realtime system  (Thermo Fisher)


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    Name:
    7900HT Fast Real Time Service Upgrade
    Description:
    The 7900HT Fast Real Time Service Upgrade allows existing 7900HT customers to upgrade their system for use with Fast thermal cycling runs This item should only be ordered if the customer has a GX270 or higher computer Customers with older computers must order the 7900HT Fast Real Time Service Upgrade with Computer 4362078 The 7900HT Fast Real Time PCR System is the only real time quantitative PCR system that combines 96 and 384 well plate compatibility and the TaqMan Low Density Array with fully automated robotic loading and now also offers optional Fast real time PCR capability • Fast PCR option reduces run time to about 35 minutes in a standard 96 well format or about 55 minutes in a 384 well plate • Continuous wavelength detection from 500 660 nm allows the use of multiple fluorophores in a single reaction • Measure gene expression levels detect and quantitate pathogens perform allelic discrimination SNP genotyping assays as well as score the presence of gene sequences • 96 or 384 well plate compatibility including the TaqMan Low Density Array • Optional Enterprise edition software provides data analysis tools that support 21 CFR Part 11 guidelines providing data integrity and security • Hands free plate loading and unloading provides true walkaway automation allowing you to increase your lab s productivity • Proven assay development guidelines save time and moneyHigh ThroughputThe 7900HT System is a high throughput real time PCR system that detects and quantitates nucleic acid sequences An Automation Accessory combined with 384 well plate capability make the 7900HT system ideally suited to meet the high throughput requirements of today s drug discovery process Key applications include gene expression quantitation and the detection of single nucleotide polymorphisms SNPs using the fluorogenic 5 nuclease assay FlexibilityThe 7900HT system is a versatile research tool that can accommodate any real time PCR need User interchangeable thermal cycling block formats let you select the format that s right for your project using industry standard 96 and 384 well formats as well as a novel 384 well TaqMan Low Density Array and a new Fast 96 well block that reduces run times from 2 hours to about 30 minutes An easy automation upgrade path lets you add features to meet throughput demands A Fast PCR option reduces run times from 2 hours to about 30 minutes Powerful SoftwareUsing two automation software tools Plate Utility and Automation Controller most of the gene expression and SNP genotyping workflow is fully automated for high throughput applications and requires minimal user intervention You can now process 5 000 x 384 well plates 384 markers which equates to 1 92 million genotypes Using Relative Quantification RQ Manager you can also process 200 x 384 well plates 96 detectors with quadruplicate data points⁄multiplexed endogenous control which equates to 153 600 data points Fully integrated into one complete Enterprise system SNP Manager and RQ Manager eliminate data analysis bottlenecks in high throughput SNP and gene expression research by allowing significantly more plates to be analyzed simultaneously This reduces hands on analysis time and simplifies the data analysis workflow For Research Use Only Not for use in diagnostics procedures
    Catalog Number:
    4351412
    Price:
    None
    Applications:
    Fast Real-Time PCR|PCR & Real-Time PCR|Real Time PCR (qPCR)|Real Time PCR-Based Gene Expression Profiling|Real-Time PCR Instruments, Software & Calibration|Gene Expression Analysis & Genotyping
    Category:
    Instruments and Equipment
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    Structured Review

    Thermo Fisher 7900ht fast realtime system
    The 7900HT Fast Real Time Service Upgrade allows existing 7900HT customers to upgrade their system for use with Fast thermal cycling runs This item should only be ordered if the customer has a GX270 or higher computer Customers with older computers must order the 7900HT Fast Real Time Service Upgrade with Computer 4362078 The 7900HT Fast Real Time PCR System is the only real time quantitative PCR system that combines 96 and 384 well plate compatibility and the TaqMan Low Density Array with fully automated robotic loading and now also offers optional Fast real time PCR capability • Fast PCR option reduces run time to about 35 minutes in a standard 96 well format or about 55 minutes in a 384 well plate • Continuous wavelength detection from 500 660 nm allows the use of multiple fluorophores in a single reaction • Measure gene expression levels detect and quantitate pathogens perform allelic discrimination SNP genotyping assays as well as score the presence of gene sequences • 96 or 384 well plate compatibility including the TaqMan Low Density Array • Optional Enterprise edition software provides data analysis tools that support 21 CFR Part 11 guidelines providing data integrity and security • Hands free plate loading and unloading provides true walkaway automation allowing you to increase your lab s productivity • Proven assay development guidelines save time and moneyHigh ThroughputThe 7900HT System is a high throughput real time PCR system that detects and quantitates nucleic acid sequences An Automation Accessory combined with 384 well plate capability make the 7900HT system ideally suited to meet the high throughput requirements of today s drug discovery process Key applications include gene expression quantitation and the detection of single nucleotide polymorphisms SNPs using the fluorogenic 5 nuclease assay FlexibilityThe 7900HT system is a versatile research tool that can accommodate any real time PCR need User interchangeable thermal cycling block formats let you select the format that s right for your project using industry standard 96 and 384 well formats as well as a novel 384 well TaqMan Low Density Array and a new Fast 96 well block that reduces run times from 2 hours to about 30 minutes An easy automation upgrade path lets you add features to meet throughput demands A Fast PCR option reduces run times from 2 hours to about 30 minutes Powerful SoftwareUsing two automation software tools Plate Utility and Automation Controller most of the gene expression and SNP genotyping workflow is fully automated for high throughput applications and requires minimal user intervention You can now process 5 000 x 384 well plates 384 markers which equates to 1 92 million genotypes Using Relative Quantification RQ Manager you can also process 200 x 384 well plates 96 detectors with quadruplicate data points⁄multiplexed endogenous control which equates to 153 600 data points Fully integrated into one complete Enterprise system SNP Manager and RQ Manager eliminate data analysis bottlenecks in high throughput SNP and gene expression research by allowing significantly more plates to be analyzed simultaneously This reduces hands on analysis time and simplifies the data analysis workflow For Research Use Only Not for use in diagnostics procedures
    https://www.bioz.com/result/7900ht fast realtime system/product/Thermo Fisher
    Average 94 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    7900ht fast realtime system - by Bioz Stars, 2020-07
    94/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Brain tyrosinase overexpression implicates age-dependent neuromelanin production in Parkinson’s disease pathogenesis
    Article Snippet: .. Quantitative real-time PCR (qPCR) was performed with 45–50 ng of cDNA per well in technical duplicates mixed with Taqman Gene Expression Master Mix (Applied Biosystems) and Taqman gene expression assays (human tyrosinase (Tyr) Hs00165976_m1; rat Tyr Rn01511409_m1; mouse Tyr Mm00495818_m1; human tyrosine hydroxylase (TH) Hs00165941_m1; rat TH Rn00562500_m1; mouse TH Mm00447557_m1; Applied Biosystems) using standard procedures in a 7900HT Fast Real Time Instrument (Applied Biosystems). .. Thresholds cycles (Cts) for each target gene were normalized to an endogenous reference gene (Gapdh Mm99999915_g1 / Rn01775763_g1, Rpl19 Mm02601633_g1 / Rn00821265_g1 or Rplp0 Mm00725448_s1 / Rn03302271_gH for rodent samples, and HPRT1 Hs02800695_m1, GUSB Hs00939627_m1, or RPLP0 Hs04189669_g1 for human samples).

    TLDA Assay:

    Article Title: Reduced Mature MicroRNA Levels in Association with Dicer Loss in Human Temporal Lobe Epilepsy with Hippocampal Sclerosis
    Article Snippet: .. The miRNA screen was carried out on the 7900HT Fast Realtime System using TaqMan Low-Density Arrays (TLDA) (TaqMan TLDA MicroRNA Assays v1.0 containing 380 human microRNAs assays; Applied Biosystems). .. A relative fold change in expression of the target gene transcript was determined using the comparative cycle threshold method (2−ΔΔCT ). miRNAs were called present when detected under the cut-off of 35 cycles of amplification in 3 out of 4 samples (controls) or 2 out of 3 (TLE patients).

    Negative Control:

    Article Title: Whole-genome sequencing reveals complex mechanisms of intrinsic resistance to BRAF inhibition
    Article Snippet: .. All samples were tested in triplicates and H2 O was used as a negative control. qRT-PCR was carried out on an Applied Biosystems 7900HT Fast Real Time Machine (Applied Biosystems). .. Relative expression was calculated using the ΔΔC t method with GAPDH as an internal control.

    Quantitative RT-PCR:

    Article Title: Whole-genome sequencing reveals complex mechanisms of intrinsic resistance to BRAF inhibition
    Article Snippet: .. All samples were tested in triplicates and H2 O was used as a negative control. qRT-PCR was carried out on an Applied Biosystems 7900HT Fast Real Time Machine (Applied Biosystems). .. Relative expression was calculated using the ΔΔC t method with GAPDH as an internal control.

    Article Title: Transcription factors FOXA1 and FOXA2 maintain dopaminergic neuronal properties and control feeding behavior in adult mice
    Article Snippet: .. Transcript levels were quantified by rt-qPCR performed with KAPA Sybr Fast rt-qPCR (Kapa Biosystems) mix in a total volume of 20 μL on a 7900HT fast real-time machine (Applied Biosystems). .. The threshold amplification cycles (Ct) were determined by using SDS2.3 software, and transcript levels, normalized to endogenous Gapdh levels, were quantified by the 2−ΔΔCt method.

    Expressing:

    Article Title: MicroRNAs 10a and 10b are potent inducers of neuroblastoma cell differentiation through targeting of nuclear receptor corepressor 2
    Article Snippet: .. Individual qPCRs were carried out on the 7900HT Fast Realtime System (Applied Biosystems) using GAP43-, β III Tubulin-, MYCN- and NCOR2-specific Taqman gene expression assays (Applied Biosystems) and miR-10a- and -10b-specific Taqman microRNA assays (Applied Biosystems). .. RPLPO and RNU66 were used for normalization in gene and microRNA expression studies, respectively.

    Article Title: Brain tyrosinase overexpression implicates age-dependent neuromelanin production in Parkinson’s disease pathogenesis
    Article Snippet: .. Quantitative real-time PCR (qPCR) was performed with 45–50 ng of cDNA per well in technical duplicates mixed with Taqman Gene Expression Master Mix (Applied Biosystems) and Taqman gene expression assays (human tyrosinase (Tyr) Hs00165976_m1; rat Tyr Rn01511409_m1; mouse Tyr Mm00495818_m1; human tyrosine hydroxylase (TH) Hs00165941_m1; rat TH Rn00562500_m1; mouse TH Mm00447557_m1; Applied Biosystems) using standard procedures in a 7900HT Fast Real Time Instrument (Applied Biosystems). .. Thresholds cycles (Cts) for each target gene were normalized to an endogenous reference gene (Gapdh Mm99999915_g1 / Rn01775763_g1, Rpl19 Mm02601633_g1 / Rn00821265_g1 or Rplp0 Mm00725448_s1 / Rn03302271_gH for rodent samples, and HPRT1 Hs02800695_m1, GUSB Hs00939627_m1, or RPLP0 Hs04189669_g1 for human samples).

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  • 99
    Thermo Fisher 7900ht fast realtime system
    7900ht Fast Realtime System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 246 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7900ht fast realtime system/product/Thermo Fisher
    Average 99 stars, based on 246 article reviews
    Price from $9.99 to $1999.99
    7900ht fast realtime system - by Bioz Stars, 2020-07
    99/100 stars
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    Thermo Fisher 7900ht fast realtime pcr system
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