paxgene blood rna kit qiagen 763134  (Qiagen)


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    Name:
    PAXgene Blood RNA Kit
    Description:
    For isolation and purification of intracellular RNA from blood stabilized in PAXgene Blood RNA Tubes Kit contents Qiagen PAXgene Blood RNA Kit 50 PAXgene Spin Columns 2 5mL Sample 40L Elution Volume Spin Column Format Silica Technology Manual Processing 3g Yield FDA Compatible For Isolation and Purification of Intracellular RNA from Blood Stabilized in PAXgene Blood RNA Tubes Includes 50 PAXgene Spin Columns 50 PAXgene Shredder Spin Columns Processing Tubes RNase free DNase I RNase free Reagents and Buffers Benefits For in vitro diagnostics with PAXgene Blood RNA Tubes Integrated system for collection stabilization and purification RNA stabilization for up to 3 days at 18 25°C Stabilization for at least 60 months at 20°C or 70°C Standardized sample processing prior to analysis
    Catalog Number:
    762164
    Price:
    554
    Category:
    PAXgene Blood RNA Kit IVD
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    Structured Review

    Qiagen paxgene blood rna kit qiagen 763134
    PAXgene Blood RNA Kit
    For isolation and purification of intracellular RNA from blood stabilized in PAXgene Blood RNA Tubes Kit contents Qiagen PAXgene Blood RNA Kit 50 PAXgene Spin Columns 2 5mL Sample 40L Elution Volume Spin Column Format Silica Technology Manual Processing 3g Yield FDA Compatible For Isolation and Purification of Intracellular RNA from Blood Stabilized in PAXgene Blood RNA Tubes Includes 50 PAXgene Spin Columns 50 PAXgene Shredder Spin Columns Processing Tubes RNase free DNase I RNase free Reagents and Buffers Benefits For in vitro diagnostics with PAXgene Blood RNA Tubes Integrated system for collection stabilization and purification RNA stabilization for up to 3 days at 18 25°C Stabilization for at least 60 months at 20°C or 70°C Standardized sample processing prior to analysis
    https://www.bioz.com/result/paxgene blood rna kit qiagen 763134/product/Qiagen
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    paxgene blood rna kit qiagen 763134 - by Bioz Stars, 2020-01
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    Clone Assay:

    Article Title: Absence of an Orphan Mitochondrial Protein, C19orf12, Causes a Distinct Clinical Subtype of Neurodegeneration with Brain Iron Accumulation
    Article Snippet: Paragraph title: Cloning of C19orf12 Transcripts ... Total RNA was isolated from human fibroblasts (NDHF-neo, Lonza, Basel, Switzerland) with the Trizol method (Invitrogen, Carlsbad, CA) or from human blood with PAXgene Blood RNA tubes (PreAnalytiX, Hombrechtikon, Switzerland) and the PAXgene Blood RNA kit (QIAGEN).

    Amplification:

    Article Title: Markers of circulating tumour cells in the peripheral blood of patients with melanoma correlate with disease recurrence and progression
    Article Snippet: Paragraph title: Blood collection, RNA isolation and amplification protocols ... Total RNA was isolated using the PAXgene Blood RNA Kit (Qiagen, Hilden, Germany) and DNase treated (DNA-free Kit, Ambion, Carlsbad, CA, U.S.A.).

    Article Title: Absence of an Orphan Mitochondrial Protein, C19orf12, Causes a Distinct Clinical Subtype of Neurodegeneration with Brain Iron Accumulation
    Article Snippet: Total RNA was isolated from human fibroblasts (NDHF-neo, Lonza, Basel, Switzerland) with the Trizol method (Invitrogen, Carlsbad, CA) or from human blood with PAXgene Blood RNA tubes (PreAnalytiX, Hombrechtikon, Switzerland) and the PAXgene Blood RNA kit (QIAGEN). .. C19orf12 coding variants 1 and 2 ( and , respectively) were amplified with Thermo-Start Taq DNA Polymerase (ABgene, Epsom, UK).

    Article Title: Supplementing Ca salts of soybean oil after artificial insemination increases pregnancy success in Bos taurus beef cows
    Article Snippet: Responses from genes of interest were quantified based on the threshold cycle ( C T ), the number of PCR cycles required for target amplification to reach a predetermined threshold. .. Total RNA was extracted from whole-blood samples using the PAXgene Blood RNA Kit (Qiagen, Valencia, CA).

    Synthesized:

    Article Title: Complement C1q expression in Erythema nodosum leprosum
    Article Snippet: RNA isolation and reverse transcription Isolation of RNA from peripheral blood and skin biopsies stored in RNAlater (Ambion, Austin, Texas) was performed using PAXgene Blood RNA Kit and RNeasy Fibrous Tissue Kit (QIAGEN Crawley, West Sussex, United Kingdom) respectively according to the manufacturer’s protocol. .. For all samples Complementary DNA (cDNA was synthesized on the same day to avoid the risk of RNA degrades during storage. cDNA was synthesised from RNA (200 ng/reaction mixture) using High Capacity cDNA Reverse Transcriptase Kit (AB Applied Biosystems, UK).

    Cytometry:

    Article Title: Apoptotic cell–induced, TLR9-dependent AhR activity is required for immunological tolerance and suppression of systemic lupus erythematosus in mice and humans
    Article Snippet: PBMCs were isolated by Percoll gradient (GE Healthcare Life Sciences) and stained for flow cytometry sorting as described elsewhere in Methods. .. Total RNA was isolated from blood archived in PAXgene tubes, collected at the time of a regular follow-up visit, utilizing the PAXgene Blood RNA Kit (Qiagen) with modifications to improve RNA yield and quality; addition of RNAse inhibitor, an off-column DNAse I digestion and final ethanol precipitation.

    Electrophoresis:

    Article Title: Characterization of intragenic tandem duplication in the PAFAH1B1 gene leading to isolated lissencephaly sequence
    Article Snippet: RNA isolation and RT-PCR Total RNA was extracted from the peripheral blood leukocytes using the PAXgene Blood RNA Kit (Qiagen GmbH; Hilden, Germany). .. The PCR products were visualized by ethidium bromide staining, following electrophoresis on 2 % agarose gels.

    Microarray:

    Article Title: Assessment and diagnostic relevance of novel serum biomarkers for early decision of ST-elevation myocardial infarction
    Article Snippet: Total RNA from blood was extracted using the PAXgene blood RNA kit (Qiagen) and purified with RNeasy kit (Qiagen) according to the manufacturer's instruction. .. Microarray analysis was performed using Sentrix HumanRef-6 Expression BeadChip or HumanHT-12 v3 Expression BeadChip (Illumina, Inc., San Diego, CA).

    Article Title: Sarcoidosis Blood Transcriptome Reflects Lung Inflammation and Overlaps with Tuberculosis
    Article Snippet: For the blood gene expression derivation set, we isolated whole blood RNA using PAXgene RNA tubes (Becton Dickinson, Franklin Lakes, NJ) and isolation kits (PAXgene Blood RNA Kit; Qiagen, Valencia, CA). .. After RNA quality assessment (Agilent Bioanalyzer 2100; Agilent Technologies, Santa Clara, CA), hybridizations were performed at the Gladstone Institute Microarray Core Facility using Affymetrix U133 Plus 2.0 microarrays (Affymetrix Inc., Santa Clara, CA).

    Article Title: Evaluation of the immunobiological effects of a regenerative far-infrared heating system in pigs
    Article Snippet: RNA preparation, labeling and purification Total RNA was isolated from the whole blood of experimental pigs using a PAXgene Blood RNA Kit (Qiagen, Germany) according to the manufacturer's protocol. .. RNA were analyzed using the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology, V6.5 2010; Agilent Technologies, Inc., USA).

    Article Title: Genome-wide expression profiling of human blood reveals biomarkers for Huntington's disease
    Article Snippet: Total RNA from blood was extracted by using the PAXgene blood RNA kit (Qiagen, Valencia, CA), and the RNeasy lipid tissue kit (Qiagen) was used for the isolation of total RNA from striatum according to the manufacturer's protocol. .. Microarray analysis was performed by using U133A GeneChips (Affymetrix) and CodeLink Uniset Human I and II bioarrays (Amersham Biosciences).

    Expressing:

    Article Title: Assessment and diagnostic relevance of novel serum biomarkers for early decision of ST-elevation myocardial infarction
    Article Snippet: Paragraph title: RNA isolation and blood whole genome expression analysis ... Total RNA from blood was extracted using the PAXgene blood RNA kit (Qiagen) and purified with RNeasy kit (Qiagen) according to the manufacturer's instruction.

    Article Title: Sarcoidosis Blood Transcriptome Reflects Lung Inflammation and Overlaps with Tuberculosis
    Article Snippet: .. For the blood gene expression derivation set, we isolated whole blood RNA using PAXgene RNA tubes (Becton Dickinson, Franklin Lakes, NJ) and isolation kits (PAXgene Blood RNA Kit; Qiagen, Valencia, CA). .. After RNA quality assessment (Agilent Bioanalyzer 2100; Agilent Technologies, Santa Clara, CA), hybridizations were performed at the Gladstone Institute Microarray Core Facility using Affymetrix U133 Plus 2.0 microarrays (Affymetrix Inc., Santa Clara, CA).

    Article Title: Identification and Characterization of an Inborn Error of Metabolism Caused by Dihydrofolate Reductase Deficiency
    Article Snippet: We assessed the expression profile of DHFR in human tissues by quantitative real-time PCR analysis (qPCR), using SYBR Green and Taqman (both from Applied Biosystems, Foster City, CA, USA) techniques. .. Whole-blood RNA was isolated from the proband, his parents, and three healthy control individuals with the use of the PAXgene Blood RNA Kit (QIAGEN, Venlo, The Netherlands).

    Article Title: Evaluation of the immunobiological effects of a regenerative far-infrared heating system in pigs
    Article Snippet: RNA preparation, labeling and purification Total RNA was isolated from the whole blood of experimental pigs using a PAXgene Blood RNA Kit (Qiagen, Germany) according to the manufacturer's protocol. .. RNA were analyzed using the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology, V6.5 2010; Agilent Technologies, Inc., USA).

    Article Title: Genome-wide expression profiling of human blood reveals biomarkers for Huntington's disease
    Article Snippet: In addition, gene expression levels of known markers of lymphocytes (IFN-induced genes), neutrophils (lactotransferrin), and reticulocytes (hemoglobin δ) exhibited no systematic differences between the HD and control samples. .. Total RNA from blood was extracted by using the PAXgene blood RNA kit (Qiagen, Valencia, CA), and the RNeasy lipid tissue kit (Qiagen) was used for the isolation of total RNA from striatum according to the manufacturer's protocol.

    Real-time Polymerase Chain Reaction:

    Article Title: Persistent Inflammatory Activity in Blood Cells and Artery Tissue from Patients with Previous Bare Metal Stent
    Article Snippet: .. RNA isolation, reverse transcription and real-time polymerase chain reaction (PCR) Total RNA was extracted from peripheral blood collected in PAXgene tubes (PreAnalytiX®, BD Company, UK) using PAXgene blood RNA kit (QIAGEN GmbH, Hilden, Germany), being then quantified using Qubit® 2.0 fluorometer (Life Technologies, Inc., Grand Island, NY). .. The RNA integrity was performed using Tapestation® 2200 and R6K Screen Tape (Agilent Technologies, Inc. UK).

    Article Title: Identification and Characterization of an Inborn Error of Metabolism Caused by Dihydrofolate Reductase Deficiency
    Article Snippet: Total RNA for qPCR analyses from different adult human brain areas was purchased from Stratagene (La Jolla, CA, USA), except for hippocampus, thalamus, and spinal cord total RNA, which was ordered from Biochain (Hayward, CA, USA). .. Whole-blood RNA was isolated from the proband, his parents, and three healthy control individuals with the use of the PAXgene Blood RNA Kit (QIAGEN, Venlo, The Netherlands).

    Derivative Assay:

    Article Title: Apoptotic cell–induced, TLR9-dependent AhR activity is required for immunological tolerance and suppression of systemic lupus erythematosus in mice and humans
    Article Snippet: Active patients (defined as a clinical SLEDAI-2K > 0, ie. not including the complement and anti-dsDNA Ab components of the SLEDAI-2K) had SLEDAI-2K scores from 4 to 32 (mean 13.5, ), and quiescent patients had SLEDAI scores from 0 to 4 (derived from low serum complements and/or high anti-dsDNA Ab levels alone, mean 1.8, ). .. Total RNA was isolated from blood archived in PAXgene tubes, collected at the time of a regular follow-up visit, utilizing the PAXgene Blood RNA Kit (Qiagen) with modifications to improve RNA yield and quality; addition of RNAse inhibitor, an off-column DNAse I digestion and final ethanol precipitation.

    Flow Cytometry:

    Article Title: Apoptotic cell–induced, TLR9-dependent AhR activity is required for immunological tolerance and suppression of systemic lupus erythematosus in mice and humans
    Article Snippet: PBMCs were isolated by Percoll gradient (GE Healthcare Life Sciences) and stained for flow cytometry sorting as described elsewhere in Methods. .. Total RNA was isolated from blood archived in PAXgene tubes, collected at the time of a regular follow-up visit, utilizing the PAXgene Blood RNA Kit (Qiagen) with modifications to improve RNA yield and quality; addition of RNAse inhibitor, an off-column DNAse I digestion and final ethanol precipitation.

    other:

    Article Title: Inhibition of TFG function causes hereditary axon degeneration by impairing endoplasmic reticulum structure
    Article Snippet: Total RNA was prepared by applying the PAXgene blood RNA kit (Qiagen).

    Polymerase Chain Reaction:

    Article Title: Persistent Inflammatory Activity in Blood Cells and Artery Tissue from Patients with Previous Bare Metal Stent
    Article Snippet: .. RNA isolation, reverse transcription and real-time polymerase chain reaction (PCR) Total RNA was extracted from peripheral blood collected in PAXgene tubes (PreAnalytiX®, BD Company, UK) using PAXgene blood RNA kit (QIAGEN GmbH, Hilden, Germany), being then quantified using Qubit® 2.0 fluorometer (Life Technologies, Inc., Grand Island, NY). .. The RNA integrity was performed using Tapestation® 2200 and R6K Screen Tape (Agilent Technologies, Inc. UK).

    Article Title: Identification and Characterization of an Inborn Error of Metabolism Caused by Dihydrofolate Reductase Deficiency
    Article Snippet: Whole-blood RNA was isolated from the proband, his parents, and three healthy control individuals with the use of the PAXgene Blood RNA Kit (QIAGEN, Venlo, The Netherlands). .. SYBR Green-based QPCR expression analysis was performed on a 7500 Fast Real-Time PCR System with the use of Power SYBR Green PCR Master Mix (Applied Biosystems).

    Article Title: Characterization of intragenic tandem duplication in the PAFAH1B1 gene leading to isolated lissencephaly sequence
    Article Snippet: RNA isolation and RT-PCR Total RNA was extracted from the peripheral blood leukocytes using the PAXgene Blood RNA Kit (Qiagen GmbH; Hilden, Germany). .. The PCR products were visualized by ethidium bromide staining, following electrophoresis on 2 % agarose gels.

    Article Title: Supplementing Ca salts of soybean oil after artificial insemination increases pregnancy success in Bos taurus beef cows
    Article Snippet: Responses from genes of interest were quantified based on the threshold cycle ( C T ), the number of PCR cycles required for target amplification to reach a predetermined threshold. .. Total RNA was extracted from whole-blood samples using the PAXgene Blood RNA Kit (Qiagen, Valencia, CA).

    Nucleic Acid Electrophoresis:

    Article Title: Dynamic tracking of functional gene modules in treated juvenile idiopathic arthritis
    Article Snippet: RNA processing RNA was purified from whole blood PAXgene specimens using a PAXgene Blood RNA kit (Qiagen, Valencia, CA, USA) as recommended by the manufacturer, including a DNAse (Qiagen) step to remove genomic DNA. .. Final RNA preparations were suspended in RNase-free water, quantified spectrophotometrically, and analyzed for RNA integrity by capillary gel electrophoresis (Agilent 2100 Bioanalyzer; Agilent Technologies, Palo Alto, CA, USA).

    Isolation:

    Article Title: Assessment and diagnostic relevance of novel serum biomarkers for early decision of ST-elevation myocardial infarction
    Article Snippet: Paragraph title: RNA isolation and blood whole genome expression analysis ... Total RNA from blood was extracted using the PAXgene blood RNA kit (Qiagen) and purified with RNeasy kit (Qiagen) according to the manufacturer's instruction.

    Article Title: Persistent Inflammatory Activity in Blood Cells and Artery Tissue from Patients with Previous Bare Metal Stent
    Article Snippet: .. RNA isolation, reverse transcription and real-time polymerase chain reaction (PCR) Total RNA was extracted from peripheral blood collected in PAXgene tubes (PreAnalytiX®, BD Company, UK) using PAXgene blood RNA kit (QIAGEN GmbH, Hilden, Germany), being then quantified using Qubit® 2.0 fluorometer (Life Technologies, Inc., Grand Island, NY). .. The RNA integrity was performed using Tapestation® 2200 and R6K Screen Tape (Agilent Technologies, Inc. UK).

    Article Title: Sarcoidosis Blood Transcriptome Reflects Lung Inflammation and Overlaps with Tuberculosis
    Article Snippet: .. For the blood gene expression derivation set, we isolated whole blood RNA using PAXgene RNA tubes (Becton Dickinson, Franklin Lakes, NJ) and isolation kits (PAXgene Blood RNA Kit; Qiagen, Valencia, CA). .. After RNA quality assessment (Agilent Bioanalyzer 2100; Agilent Technologies, Santa Clara, CA), hybridizations were performed at the Gladstone Institute Microarray Core Facility using Affymetrix U133 Plus 2.0 microarrays (Affymetrix Inc., Santa Clara, CA).

    Article Title: Apoptotic cell–induced, TLR9-dependent AhR activity is required for immunological tolerance and suppression of systemic lupus erythematosus in mice and humans
    Article Snippet: .. Total RNA was isolated from blood archived in PAXgene tubes, collected at the time of a regular follow-up visit, utilizing the PAXgene Blood RNA Kit (Qiagen) with modifications to improve RNA yield and quality; addition of RNAse inhibitor, an off-column DNAse I digestion and final ethanol precipitation. .. Culture of Mϕ and DC Resident Mϕ were obtained from 6–8 week old female mice by peritoneal lavage with 3ml ice cold PBS and were cultured in RPMI 1640 + 10% fetal bovine serum (FBS), 100U/ml penicillin and streptomycin (ThermoFisher).

    Article Title: Identification and Characterization of an Inborn Error of Metabolism Caused by Dihydrofolate Reductase Deficiency
    Article Snippet: .. Whole-blood RNA was isolated from the proband, his parents, and three healthy control individuals with the use of the PAXgene Blood RNA Kit (QIAGEN, Venlo, The Netherlands). .. Whole-blood and cochlear RNA was treated with DNase I (Invitrogen, Leek, The Netherlands), and the concentration and purity were determined by optical densitometry.

    Article Title: Markers of circulating tumour cells in the peripheral blood of patients with melanoma correlate with disease recurrence and progression
    Article Snippet: .. Total RNA was isolated using the PAXgene Blood RNA Kit (Qiagen, Hilden, Germany) and DNase treated (DNA-free Kit, Ambion, Carlsbad, CA, U.S.A.). .. RNA was quantified and the integrity was qualified (Agilent 2100 Bioanalyser, Agilent Technologies, Santa Clara, CA, U.S.A.).

    Article Title: Evaluation of the immunobiological effects of a regenerative far-infrared heating system in pigs
    Article Snippet: .. RNA preparation, labeling and purification Total RNA was isolated from the whole blood of experimental pigs using a PAXgene Blood RNA Kit (Qiagen, Germany) according to the manufacturer's protocol. ..

    Article Title: Absence of an Orphan Mitochondrial Protein, C19orf12, Causes a Distinct Clinical Subtype of Neurodegeneration with Brain Iron Accumulation
    Article Snippet: .. Total RNA was isolated from human fibroblasts (NDHF-neo, Lonza, Basel, Switzerland) with the Trizol method (Invitrogen, Carlsbad, CA) or from human blood with PAXgene Blood RNA tubes (PreAnalytiX, Hombrechtikon, Switzerland) and the PAXgene Blood RNA kit (QIAGEN). .. One microgram of RNA was reverse transcribed with M-MLV reverse transcriptase (Promega, Madison, WI) and oligo dT.

    Article Title: Genome-wide expression profiling of human blood reveals biomarkers for Huntington's disease
    Article Snippet: .. Total RNA from blood was extracted by using the PAXgene blood RNA kit (Qiagen, Valencia, CA), and the RNeasy lipid tissue kit (Qiagen) was used for the isolation of total RNA from striatum according to the manufacturer's protocol. .. All samples were treated with the RNase-free DNase set (Qiagen).

    Article Title: Characterization of intragenic tandem duplication in the PAFAH1B1 gene leading to isolated lissencephaly sequence
    Article Snippet: .. RNA isolation and RT-PCR Total RNA was extracted from the peripheral blood leukocytes using the PAXgene Blood RNA Kit (Qiagen GmbH; Hilden, Germany). .. The RT step was performed using the SuperScript First-Strand Synthesis System (Invitrogen Corporation; Carlsbad, CA) to generate cDNA by using 1 μg of total RNA in a 20-μl reaction.

    Article Title: Supplementing Ca salts of soybean oil after artificial insemination increases pregnancy success in Bos taurus beef cows
    Article Snippet: Total RNA was extracted from whole-blood samples using the PAXgene Blood RNA Kit (Qiagen, Valencia, CA). .. Assessment of quantity and quality of isolated RNA, reverse transcription, and real-time reverse transcription–PCR with gene-specific primers (20 pM each; ) was performed as in .

    Article Title: Complement C1q expression in Erythema nodosum leprosum
    Article Snippet: .. RNA isolation and reverse transcription Isolation of RNA from peripheral blood and skin biopsies stored in RNAlater (Ambion, Austin, Texas) was performed using PAXgene Blood RNA Kit and RNeasy Fibrous Tissue Kit (QIAGEN Crawley, West Sussex, United Kingdom) respectively according to the manufacturer’s protocol. .. DNase I (QIAGEN) was included for all RNA preparations for DNA digestion.

    Labeling:

    Article Title: Assessment and diagnostic relevance of novel serum biomarkers for early decision of ST-elevation myocardial infarction
    Article Snippet: Briefly, whole blood (2.5 mL per patient) was collected directly into PAXgene Blood RNA tube, labeled with a unique identification number, stored at room temperature, and transferred to the laboratory within 4 hours for blood processing. .. Total RNA from blood was extracted using the PAXgene blood RNA kit (Qiagen) and purified with RNeasy kit (Qiagen) according to the manufacturer's instruction.

    Article Title: Evaluation of the immunobiological effects of a regenerative far-infrared heating system in pigs
    Article Snippet: .. RNA preparation, labeling and purification Total RNA was isolated from the whole blood of experimental pigs using a PAXgene Blood RNA Kit (Qiagen, Germany) according to the manufacturer's protocol. ..

    Article Title: Dynamic tracking of functional gene modules in treated juvenile idiopathic arthritis
    Article Snippet: RNA processing RNA was purified from whole blood PAXgene specimens using a PAXgene Blood RNA kit (Qiagen, Valencia, CA, USA) as recommended by the manufacturer, including a DNAse (Qiagen) step to remove genomic DNA. .. Globin transcripts, which reduce labeling efficiency of whole blood cell RNA and decrease signal-to-noise ratios on microarrays [ ] were reduced using GLOBINclear-Human (Ambion, Austin, TX, USA).

    Purification:

    Article Title: Assessment and diagnostic relevance of novel serum biomarkers for early decision of ST-elevation myocardial infarction
    Article Snippet: .. Total RNA from blood was extracted using the PAXgene blood RNA kit (Qiagen) and purified with RNeasy kit (Qiagen) according to the manufacturer's instruction. .. The quality of total RNA was analyzed with the RNA StdSens Chips on the Experion™ system (BioRad, Hercules, CA).

    Article Title: Evaluation of the immunobiological effects of a regenerative far-infrared heating system in pigs
    Article Snippet: .. RNA preparation, labeling and purification Total RNA was isolated from the whole blood of experimental pigs using a PAXgene Blood RNA Kit (Qiagen, Germany) according to the manufacturer's protocol. ..

    Article Title: Dynamic tracking of functional gene modules in treated juvenile idiopathic arthritis
    Article Snippet: .. RNA processing RNA was purified from whole blood PAXgene specimens using a PAXgene Blood RNA kit (Qiagen, Valencia, CA, USA) as recommended by the manufacturer, including a DNAse (Qiagen) step to remove genomic DNA. .. Globin transcripts, which reduce labeling efficiency of whole blood cell RNA and decrease signal-to-noise ratios on microarrays [ ] were reduced using GLOBINclear-Human (Ambion, Austin, TX, USA).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Characterization of intragenic tandem duplication in the PAFAH1B1 gene leading to isolated lissencephaly sequence
    Article Snippet: .. RNA isolation and RT-PCR Total RNA was extracted from the peripheral blood leukocytes using the PAXgene Blood RNA Kit (Qiagen GmbH; Hilden, Germany). .. The RT step was performed using the SuperScript First-Strand Synthesis System (Invitrogen Corporation; Carlsbad, CA) to generate cDNA by using 1 μg of total RNA in a 20-μl reaction.

    Mouse Assay:

    Article Title: Transcriptomics of the Vaccine Immune Response: Priming With Adjuvant Modulates Recall Innate Responses After Boosting
    Article Snippet: For transcriptomic analysis, blood samples were collected from individual mice on days 1, 2, 7, 28, 29, 30, and 35 after priming. .. Total RNA was extracted using the PaxGene blood RNA kit (Qiagen, Germany) following manufacturer’s instructions and resuspended in 60 µl of BR5 buffer.

    SYBR Green Assay:

    Article Title: Identification and Characterization of an Inborn Error of Metabolism Caused by Dihydrofolate Reductase Deficiency
    Article Snippet: We assessed the expression profile of DHFR in human tissues by quantitative real-time PCR analysis (qPCR), using SYBR Green and Taqman (both from Applied Biosystems, Foster City, CA, USA) techniques. .. Whole-blood RNA was isolated from the proband, his parents, and three healthy control individuals with the use of the PAXgene Blood RNA Kit (QIAGEN, Venlo, The Netherlands).

    Multiplex Assay:

    Article Title: Persistent Inflammatory Activity in Blood Cells and Artery Tissue from Patients with Previous Bare Metal Stent
    Article Snippet: RNA isolation, reverse transcription and real-time polymerase chain reaction (PCR) Total RNA was extracted from peripheral blood collected in PAXgene tubes (PreAnalytiX®, BD Company, UK) using PAXgene blood RNA kit (QIAGEN GmbH, Hilden, Germany), being then quantified using Qubit® 2.0 fluorometer (Life Technologies, Inc., Grand Island, NY). .. Real-time qPCR was carried out in Rotor-Gene® detection system using the TaqMan® Fast Multiplex PCR kit (QIAGEN GmbH, Hilden, Germany) and primers from Applied Biosystem commercially designed for TaqMan® qPCR (Applied Biosystems, Foster, CA, USA).

    Agarose Gel Electrophoresis:

    Article Title: Complement C1q expression in Erythema nodosum leprosum
    Article Snippet: RNA isolation and reverse transcription Isolation of RNA from peripheral blood and skin biopsies stored in RNAlater (Ambion, Austin, Texas) was performed using PAXgene Blood RNA Kit and RNeasy Fibrous Tissue Kit (QIAGEN Crawley, West Sussex, United Kingdom) respectively according to the manufacturer’s protocol. .. RNA yield was determined using a NanoDrop 2000, spectrophotometer (Thermo Scientific, Epsom, UK) and integrity was checked by agarose gel electrophoresis.

    Ethanol Precipitation:

    Article Title: Apoptotic cell–induced, TLR9-dependent AhR activity is required for immunological tolerance and suppression of systemic lupus erythematosus in mice and humans
    Article Snippet: .. Total RNA was isolated from blood archived in PAXgene tubes, collected at the time of a regular follow-up visit, utilizing the PAXgene Blood RNA Kit (Qiagen) with modifications to improve RNA yield and quality; addition of RNAse inhibitor, an off-column DNAse I digestion and final ethanol precipitation. .. Culture of Mϕ and DC Resident Mϕ were obtained from 6–8 week old female mice by peritoneal lavage with 3ml ice cold PBS and were cultured in RPMI 1640 + 10% fetal bovine serum (FBS), 100U/ml penicillin and streptomycin (ThermoFisher).

    Spectrophotometry:

    Article Title: Evaluation of the immunobiological effects of a regenerative far-infrared heating system in pigs
    Article Snippet: RNA preparation, labeling and purification Total RNA was isolated from the whole blood of experimental pigs using a PAXgene Blood RNA Kit (Qiagen, Germany) according to the manufacturer's protocol. .. RNA purity and integrity were measured by the OD 260/280 ratio of ND-1000 spectrophotometer (NanoDrop, USA) for quality control.

    Article Title: Complement C1q expression in Erythema nodosum leprosum
    Article Snippet: RNA isolation and reverse transcription Isolation of RNA from peripheral blood and skin biopsies stored in RNAlater (Ambion, Austin, Texas) was performed using PAXgene Blood RNA Kit and RNeasy Fibrous Tissue Kit (QIAGEN Crawley, West Sussex, United Kingdom) respectively according to the manufacturer’s protocol. .. RNA yield was determined using a NanoDrop 2000, spectrophotometer (Thermo Scientific, Epsom, UK) and integrity was checked by agarose gel electrophoresis.

    Concentration Assay:

    Article Title: Identification and Characterization of an Inborn Error of Metabolism Caused by Dihydrofolate Reductase Deficiency
    Article Snippet: Whole-blood RNA was isolated from the proband, his parents, and three healthy control individuals with the use of the PAXgene Blood RNA Kit (QIAGEN, Venlo, The Netherlands). .. Whole-blood and cochlear RNA was treated with DNase I (Invitrogen, Leek, The Netherlands), and the concentration and purity were determined by optical densitometry.

    Staining:

    Article Title: Apoptotic cell–induced, TLR9-dependent AhR activity is required for immunological tolerance and suppression of systemic lupus erythematosus in mice and humans
    Article Snippet: PBMCs were isolated by Percoll gradient (GE Healthcare Life Sciences) and stained for flow cytometry sorting as described elsewhere in Methods. .. Total RNA was isolated from blood archived in PAXgene tubes, collected at the time of a regular follow-up visit, utilizing the PAXgene Blood RNA Kit (Qiagen) with modifications to improve RNA yield and quality; addition of RNAse inhibitor, an off-column DNAse I digestion and final ethanol precipitation.

    Article Title: Characterization of intragenic tandem duplication in the PAFAH1B1 gene leading to isolated lissencephaly sequence
    Article Snippet: RNA isolation and RT-PCR Total RNA was extracted from the peripheral blood leukocytes using the PAXgene Blood RNA Kit (Qiagen GmbH; Hilden, Germany). .. The PCR products were visualized by ethidium bromide staining, following electrophoresis on 2 % agarose gels.

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    Qiagen paxgene blood mirna kit
    Paxgene Blood Mirna Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/paxgene blood mirna kit/product/Qiagen
    Average 90 stars, based on 89 article reviews
    Price from $9.99 to $1999.99
    paxgene blood mirna kit - by Bioz Stars, 2020-01
    90/100 stars
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