paxgene blood rna kit qiagen 763134  (Qiagen)


Bioz Verified Symbol Qiagen is a verified supplier
Bioz Manufacturer Symbol Qiagen manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    PAXgene Blood miRNA Kit
    Description:
    For purification of miRNA and total RNA from whole blood. Kit contents: Qiagen PAXgene Blood miRNA Kit, 50 preps, 2.5mL Sample, 40L Elution Volume, Whole Blood Sample, Silica Technology, Spin Column Format, Manual Processing, Ideal for RT-PCR and Real-time RT-PCR, cDNA synthesis, Microarray Analysis, Primer Extension, RNase/S1 Nuclease Protection, For Purification of miRNA and Total RNA from Whole Blood, Includes PAXgene Spin Columns, PAXgene Shredder Spin Columns, Processing Tubes, Microcentrifuge Tubes, RNase-free DNase, RNase-free Reagents and Buffers. Benefits: Integrated system for collection, stabilization, and purification . Effective purification of miRNA and total RNA . RNA stabilization for up to 3 days at 18–25°C. Stabilization for at least 50 months at –20°C or –70°C
    Catalog Number:
    763134
    Price:
    None
    Category:
    PAXgene Blood miRNA Kit
    Score:
    85
    Buy from Supplier


    Structured Review

    Qiagen paxgene blood rna kit qiagen 763134
    PAXgene Blood miRNA Kit
    For purification of miRNA and total RNA from whole blood. Kit contents: Qiagen PAXgene Blood miRNA Kit, 50 preps, 2.5mL Sample, 40L Elution Volume, Whole Blood Sample, Silica Technology, Spin Column Format, Manual Processing, Ideal for RT-PCR and Real-time RT-PCR, cDNA synthesis, Microarray Analysis, Primer Extension, RNase/S1 Nuclease Protection, For Purification of miRNA and Total RNA from Whole Blood, Includes PAXgene Spin Columns, PAXgene Shredder Spin Columns, Processing Tubes, Microcentrifuge Tubes, RNase-free DNase, RNase-free Reagents and Buffers. Benefits: Integrated system for collection, stabilization, and purification . Effective purification of miRNA and total RNA . RNA stabilization for up to 3 days at 18–25°C. Stabilization for at least 50 months at –20°C or –70°C
    https://www.bioz.com/result/paxgene blood rna kit qiagen 763134/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    paxgene blood rna kit qiagen 763134 - by Bioz Stars, 2019-12
    99/100 stars

    Images

    Related Articles

    RNA Extraction:

    Article Title: A Two-Cohort RNA-seq Study Reveals Changes in Endometrial and Blood miRNome in Fertile and Infertile Women
    Article Snippet: Paragraph title: 2.4. RNA Extraction from Blood Samples ... In EST, RNA was extracted from the whole blood samples using PAXgene Blood miRNA Kit (Qiagen) according to manufacturer’s instructions.

    Article Title: Differences in miRNA differential expression in whole blood between horses with sarcoid regression and progression. Differences in miRNA differential expression in whole blood between horses with sarcoid regression and progression
    Article Snippet: Samples were handled according to a previously established protocol for long‐term stored equine whole blood. .. The blood was gently thawed on ice and transferred to a PAXgene blood RNA tube 16 hours before the start of RNA extraction with a PAXgene Blood miRNA Kit (Qiagen, Hombrechtikon, Switzerland). .. The RNA concentrations were measured with Qubit RNA BR assay kit (ThermoFisher, Basel, Switzerland).

    Amplification:

    Article Title: Expression of mitochondrial dysfunction-related genes and pathways in paclitaxel-induced peripheral neuropathy in breast cancer survivors
    Article Snippet: Total RNA was isolated using the PAXgene® Blood miRNA Kit (Qiagen, Inc.) using established procedures. .. Prior to library preparation, 600 ng of total RNA was treated with the Illumina Globin-Zero Gold rRNA Removal Kit (Illumina Inc., San Diego, CA) to deplete cytoplasmic ribosomal RNA and human globin mRNA., The globin/ribo depleted RNA was cleaned with Agencourt RNAClean XP (Beckman Coulter, Indianapolis, IN), and the sequencing libraries were prepared with KAPA RNA HyperPrep Kit (Roche Diagnostics Corp., Indianapolis, IN) according to the manufacturer’s protocol.

    Article Title: Extensive alterations of the whole-blood transcriptome are associated with body mass index: results of an mRNA profiling study involving two large population-based cohorts
    Article Snippet: Subsequently, RNA was prepared using the PAXgene™ Blood miRNA Kit (QIAGEN, Hilden, Germany). .. Subsequently, RNA was prepared using the PAXgene™ Blood miRNA Kit (QIAGEN, Hilden, Germany).

    Sample Prep:

    Article Title: Expression of mitochondrial dysfunction-related genes and pathways in paclitaxel-induced peripheral neuropathy in breast cancer survivors
    Article Snippet: Paragraph title: RNA sample preparation ... Total RNA was isolated using the PAXgene® Blood miRNA Kit (Qiagen, Inc.) using established procedures.

    Article Title: Differences in miRNA differential expression in whole blood between horses with sarcoid regression and progression. Differences in miRNA differential expression in whole blood between horses with sarcoid regression and progression
    Article Snippet: Paragraph title: 2.2. RNA sample preparation and sequencing of multiplexed small RNA samples ... The blood was gently thawed on ice and transferred to a PAXgene blood RNA tube 16 hours before the start of RNA extraction with a PAXgene Blood miRNA Kit (Qiagen, Hombrechtikon, Switzerland).

    Article Title: A comprehensive, cell specific microRNA catalogue of human peripheral blood
    Article Snippet: For whole blood, we used the PAXgene Blood miRNA Kit (Qiagen). .. For whole blood, we used the PAXgene Blood miRNA Kit (Qiagen).

    RNA HS Assay:

    Article Title: Cellular and extracellular mi RNAs are blood‐compartment‐specific diagnostic targets in sepsis
    Article Snippet: For extraction of blood cell RNA, PAXgene blood tubes were processed with the PAXgene blood miRNA kit (Qiagen) according to the manufacturer's protocol. .. For extraction of blood cell RNA, PAXgene blood tubes were processed with the PAXgene blood miRNA kit (Qiagen) according to the manufacturer's protocol.

    Mutagenesis:

    Article Title: Recessive Mutations in the α3 (VI) Collagen Gene COL6A3 Cause Early-Onset Isolated Dystonia
    Article Snippet: The effect of the canonical COL6A3 exon 41 splice site mutation c.8966−1G > C on mRNA transcript was analyzed by cDNA sequencing. .. RNA was isolated from whole blood of three case subjects and a control individual via the PAXgene Blood miRNA kit from QIAGEN.

    Isolation:

    Article Title: Expression of mitochondrial dysfunction-related genes and pathways in paclitaxel-induced peripheral neuropathy in breast cancer survivors
    Article Snippet: A p value of < .05 was considered statistically significant. .. Total RNA was isolated using the PAXgene® Blood miRNA Kit (Qiagen, Inc.) using established procedures. .. Total RNA from the 50 survivors was sent to the UC Davis Genomics Core Facility for library preparation and for sequencing.

    Article Title: MicroRNA Expression Profiles of Whole Blood in Lung Adenocarcinoma
    Article Snippet: For controls, blood counts and hemoglobin values could be obtained for 17 (74%) subjects; for six of them, the values were determined > 90 days before blood had been collected for RNA isolation. .. Total RNA including small RNA was isolated from blood collected in PAXgene™ Blood RNA tubes using the PAXgene™ Blood miRNA kit (Qiagen®) as per the protocol supplied by the manufacturer. .. RNA was collected in 80 µl of the BR5 buffer provided with the kit.

    Article Title: High-throughput qRT-PCR validation of blood microRNAs in non-small cell lung cancer
    Article Snippet: Total RNA using miRNA from PAXgene RNA blood collection tubes was extracted either with the miRNeasy Mini Kit (Qiagen) or the PAXgene Blood miRNA Kit (Qiagen). .. Total RNA using miRNA from PAXgene RNA blood collection tubes was extracted either with the miRNeasy Mini Kit (Qiagen) or the PAXgene Blood miRNA Kit (Qiagen).

    Article Title: Genome-wide expression and response to exposure-based psychological therapy for anxiety disorders
    Article Snippet: Whole blood samples were drawn at pre-treatment (before exposure), immediately post treatment and follow-up (~6 months following the conclusion of treatment) using PAXgene blood RNA tubes. .. Blood RNA was isolated and purified using the PAXgene Blood miRNA Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol using the Qiagen Qiacube. .. RNA quality and integrity were measured using both the Nanodrop 1000 spectrophotometer (Nanodrop 1000, Nanodrop, Wilmington, DE, USA) and the Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA, USA).

    Article Title: Low variance RNAs identify Parkinson’s disease molecular signature in blood
    Article Snippet: Paragraph title: RNA isolation ... Total RNA was extracted using the PAXgene® blood miRNA kit, following the manufacturer’s instructions (Qiagen).

    Article Title: Impact of common regulatory single-nucleotide variants on gene expression profiles in whole blood
    Article Snippet: RNA was prepared from whole blood under fasting conditions in PAXgene tubes (PreAnalytiX) using the PAXgene Blood miRNA Kit (Qiagen) on a QIAcube (Qiagen). .. DNA was isolated using the Gentra Puregene Blood Kit (Qiagen).

    Article Title: The blood-borne miRNA signature of lung cancer patients is independent of histology but influenced by metastases
    Article Snippet: Informed consent was obtained from each study subject and the study was approved by the local ethics committee (Ärztekammer des Saarlandes; ID 01/08). .. Total RNA including miRNA was isolated using the PAXgene Blood miRNA Kit (Qiagen) following the manufacturers recommendations. .. Isolated RNA was stored at -80C.

    Article Title: Phase 1 Study of a Sulforaphane-Containing Broccoli Sprout Homogenate for Sickle Cell Disease
    Article Snippet: Paragraph title: RNA isolation ... Blood samples were drawn into PAXgene Blood RNA tubes (Qiagen), and RNA was purified from whole blood using the PAXgene Blood miRNA Kit (Qiagen) according to the manufacturer’s protocol.

    Article Title: MicroRNAs 146a/b-5 and 425-3p and 24-3p are markers of antidepressant response and regulate MAPK/Wnt-system genes
    Article Snippet: PAXgene tubes were frozen using a sequential freezing process: tubes were stored at room temperature for 3 h, transferred to 4 °C overnight, followed by 6–8 h at −20 °C and then final storage at −80 °C. .. Total RNA (including the miRNA fraction) was isolated from whole blood using the PAXgene Blood miRNA Kit (Qiagen, Canada) according to the manufacturer's instructions. .. Brain tissues were processed and dissected at 4 °C, then snap-frozen in liquid nitrogen before storage at −80 °C.

    Article Title: Cellular and extracellular mi RNAs are blood‐compartment‐specific diagnostic targets in sepsis
    Article Snippet: Serum of 600 μl were extracted with the miRCURY™ RNA Isolation Kit—Biofluids (Exiqon) according to the manufacturer's protocol. .. For extraction of blood cell RNA, PAXgene blood tubes were processed with the PAXgene blood miRNA kit (Qiagen) according to the manufacturer's protocol.

    Article Title: A comprehensive, cell specific microRNA catalogue of human peripheral blood
    Article Snippet: Paragraph title: Total RNA isolation and sRNA-Seq ... For whole blood, we used the PAXgene Blood miRNA Kit (Qiagen).

    Article Title: Recessive Mutations in the α3 (VI) Collagen Gene COL6A3 Cause Early-Onset Isolated Dystonia
    Article Snippet: The effect of the canonical COL6A3 exon 41 splice site mutation c.8966−1G > C on mRNA transcript was analyzed by cDNA sequencing. .. RNA was isolated from whole blood of three case subjects and a control individual via the PAXgene Blood miRNA kit from QIAGEN. .. Blood total RNA was reverse transcribed to cDNA with the SuperScript First-Strand Synthesis System for RT-PCR (Invitrogen), and the relevant cDNA fragment was amplified with specific primers ( ).

    Article Title: Multi-omics monitoring of drug response in rheumatoid arthritis in pursuit of molecular remission
    Article Snippet: For whole-blood transcriptome analysis, blood samples collected from healthy human donors and individuals with RA in PAXgene tubes were frozen and stored at −80 °C. .. Total RNA was isolated using the PAXgene Blood miRNA Kit (763134, Qiagen, Valencia, CA, USA). .. Globin-encoding transcripts were removed from the total RNA by using the Ambion GLOBINclear kit (AM1980, Ambion, Austin TX, USA).

    Sequencing:

    Article Title: Expression of mitochondrial dysfunction-related genes and pathways in paclitaxel-induced peripheral neuropathy in breast cancer survivors
    Article Snippet: Total RNA was isolated using the PAXgene® Blood miRNA Kit (Qiagen, Inc.) using established procedures. .. Total RNA from the 50 survivors was sent to the UC Davis Genomics Core Facility for library preparation and for sequencing.

    Article Title: Differences in miRNA differential expression in whole blood between horses with sarcoid regression and progression. Differences in miRNA differential expression in whole blood between horses with sarcoid regression and progression
    Article Snippet: Paragraph title: 2.2. RNA sample preparation and sequencing of multiplexed small RNA samples ... The blood was gently thawed on ice and transferred to a PAXgene blood RNA tube 16 hours before the start of RNA extraction with a PAXgene Blood miRNA Kit (Qiagen, Hombrechtikon, Switzerland).

    Article Title: Recessive Mutations in the α3 (VI) Collagen Gene COL6A3 Cause Early-Onset Isolated Dystonia
    Article Snippet: The effect of the canonical COL6A3 exon 41 splice site mutation c.8966−1G > C on mRNA transcript was analyzed by cDNA sequencing. .. RNA was isolated from whole blood of three case subjects and a control individual via the PAXgene Blood miRNA kit from QIAGEN.

    Multiplex Assay:

    Article Title: Differences in miRNA differential expression in whole blood between horses with sarcoid regression and progression. Differences in miRNA differential expression in whole blood between horses with sarcoid regression and progression
    Article Snippet: The blood was gently thawed on ice and transferred to a PAXgene blood RNA tube 16 hours before the start of RNA extraction with a PAXgene Blood miRNA Kit (Qiagen, Hombrechtikon, Switzerland). .. The blood was gently thawed on ice and transferred to a PAXgene blood RNA tube 16 hours before the start of RNA extraction with a PAXgene Blood miRNA Kit (Qiagen, Hombrechtikon, Switzerland).

    Spectrophotometry:

    Article Title: Impact of common regulatory single-nucleotide variants on gene expression profiles in whole blood
    Article Snippet: RNA was prepared from whole blood under fasting conditions in PAXgene tubes (PreAnalytiX) using the PAXgene Blood miRNA Kit (Qiagen) on a QIAcube (Qiagen). .. Isolation of both RNA and DNA was performed according to the Qiagen protocols.

    Purification:

    Article Title: Evaluation of circulating miRNAs during late pregnancy in the mare
    Article Snippet: All mares were confirmed to have normal pregnancies by ultrasonography at the sampling times and thorough pathologic placental evaluation postpartum. .. RNA was purified using the PAXgene blood miRNA kit (Qiagen, Germantown, MD, USA) per manufacturer’s instructions, then stored at -20°C until use. .. RNA purity and concentration were assessed using the NanoDrop 2000 spectrophotometer (ThermoScientific, Waltham, MA, USA), with 260/280 ratios above 1.8 and 260/230 ratios above 2.0 being considered acceptable.

    Article Title: Genome-wide expression and response to exposure-based psychological therapy for anxiety disorders
    Article Snippet: Whole blood samples were drawn at pre-treatment (before exposure), immediately post treatment and follow-up (~6 months following the conclusion of treatment) using PAXgene blood RNA tubes. .. Blood RNA was isolated and purified using the PAXgene Blood miRNA Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol using the Qiagen Qiacube. .. RNA quality and integrity were measured using both the Nanodrop 1000 spectrophotometer (Nanodrop 1000, Nanodrop, Wilmington, DE, USA) and the Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA, USA).

    Article Title: Phase 1 Study of a Sulforaphane-Containing Broccoli Sprout Homogenate for Sickle Cell Disease
    Article Snippet: Cells were treated with SFN (Calbiochem) or tert-Butylhydroquinone (TBHQ) (Sigma) in DMSO. .. Blood samples were drawn into PAXgene Blood RNA tubes (Qiagen), and RNA was purified from whole blood using the PAXgene Blood miRNA Kit (Qiagen) according to the manufacturer’s protocol. .. RNA from in vitro cultured erythroid progenitors was isolated using the miRVana miRNA isolation kit (Applied Biosystems), according to the manufacturer’s protocol.

    Article Title: MicroRNAs 146a/b-5 and 425-3p and 24-3p are markers of antidepressant response and regulate MAPK/Wnt-system genes
    Article Snippet: Total RNA (including the miRNA fraction) was isolated from whole blood using the PAXgene Blood miRNA Kit (Qiagen, Canada) according to the manufacturer's instructions. .. Total RNA was isolated from 40 mg of frozen PFC tissue using the miRNeasy Mini Kit protocol in combination with the RNeasy MinElute Cleanup Kit (Qiagen, Canada), with no modifications.

    Article Title: Multi-omics monitoring of drug response in rheumatoid arthritis in pursuit of molecular remission
    Article Snippet: Total RNA was isolated using the PAXgene Blood miRNA Kit (763134, Qiagen, Valencia, CA, USA). .. Total RNA was isolated using the PAXgene Blood miRNA Kit (763134, Qiagen, Valencia, CA, USA).

    Real-time Polymerase Chain Reaction:

    Article Title: Expression of mitochondrial dysfunction-related genes and pathways in paclitaxel-induced peripheral neuropathy in breast cancer survivors
    Article Snippet: Total RNA was isolated using the PAXgene® Blood miRNA Kit (Qiagen, Inc.) using established procedures. .. Fourteen cycles of polymerase chain reaction (PCR) amplification were used for double six base pair index addition and library fragment enrichment.

    Concentration Assay:

    Article Title: Particulate Air Pollution Exposure and Expression of Viral and Human MicroRNAs in Blood: The Beijing Truck Driver Air Pollution Study
    Article Snippet: For each element, we calculated enrichment factors (EF) for truck drivers (TD) relative to office workers (OW) as follows: EF(x)t = [x(TD) /PM2.5(TD) ]/[x(OW) /PM2.5(OW) ], where [x(TD) / PM2.5(TD) ] is the ratio of the mean concentration of element x over the mean PM2.5 mass in truck drivers, and [x(OW) /PM2.5(OW) ] is the ratio of the mean concentration of the same element x over the mean PM2.5 mass in office workers. .. Total RNA was extracted using the PAXgene Blood-RNA Kit Qiagen-763134 (Qiagen).

    Article Title: MicroRNA Expression Profiles of Whole Blood in Lung Adenocarcinoma
    Article Snippet: Total RNA including small RNA was isolated from blood collected in PAXgene™ Blood RNA tubes using the PAXgene™ Blood miRNA kit (Qiagen®) as per the protocol supplied by the manufacturer. .. Total RNA including small RNA was isolated from blood collected in PAXgene™ Blood RNA tubes using the PAXgene™ Blood miRNA kit (Qiagen®) as per the protocol supplied by the manufacturer.

    Article Title: Impact of common regulatory single-nucleotide variants on gene expression profiles in whole blood
    Article Snippet: RNA was prepared from whole blood under fasting conditions in PAXgene tubes (PreAnalytiX) using the PAXgene Blood miRNA Kit (Qiagen) on a QIAcube (Qiagen). .. Isolation of both RNA and DNA was performed according to the Qiagen protocols.

    Article Title: Differential processing of amyloid precursor protein in brain and in peripheral blood leukocytes
    Article Snippet: 2.5 mL of fresh blood was drawn into PAXgene ™ Blood RNA Tubes (Qiagen, Valencia, CA, USA) and total RNA extracted using PAXgene™ Blood miRNA Kit (Qiagen). .. 2.5 mL of fresh blood was drawn into PAXgene ™ Blood RNA Tubes (Qiagen, Valencia, CA, USA) and total RNA extracted using PAXgene™ Blood miRNA Kit (Qiagen).

    Article Title: The blood-borne miRNA signature of lung cancer patients is independent of histology but influenced by metastases
    Article Snippet: Total RNA including miRNA was isolated using the PAXgene Blood miRNA Kit (Qiagen) following the manufacturers recommendations. .. Isolated RNA was stored at -80C.

    Imaging:

    Article Title: MicroRNA Expression Profiles of Whole Blood in Lung Adenocarcinoma
    Article Snippet: Total RNA including small RNA was isolated from blood collected in PAXgene™ Blood RNA tubes using the PAXgene™ Blood miRNA kit (Qiagen®) as per the protocol supplied by the manufacturer. .. Total RNA including small RNA was isolated from blood collected in PAXgene™ Blood RNA tubes using the PAXgene™ Blood miRNA kit (Qiagen®) as per the protocol supplied by the manufacturer.

    Expressing:

    Article Title: Particulate Air Pollution Exposure and Expression of Viral and Human MicroRNAs in Blood: The Beijing Truck Driver Air Pollution Study
    Article Snippet: Total RNA was extracted using the PAXgene Blood-RNA Kit Qiagen-763134 (Qiagen). .. Total RNA was extracted using the PAXgene Blood-RNA Kit Qiagen-763134 (Qiagen).

    Article Title: Genome-wide expression and response to exposure-based psychological therapy for anxiety disorders
    Article Snippet: Blood RNA was isolated and purified using the PAXgene Blood miRNA Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol using the Qiagen Qiacube. .. RNA quality and integrity were measured using both the Nanodrop 1000 spectrophotometer (Nanodrop 1000, Nanodrop, Wilmington, DE, USA) and the Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA, USA).

    Article Title: Differential processing of amyloid precursor protein in brain and in peripheral blood leukocytes
    Article Snippet: 2.5 mL of fresh blood was drawn into PAXgene ™ Blood RNA Tubes (Qiagen, Valencia, CA, USA) and total RNA extracted using PAXgene™ Blood miRNA Kit (Qiagen). .. The quantitative RT-PCR procedure was as follows: 500 ng of total RNA was reverse transcribed with the High Capacity RNA-to-cDNA Kit (Applied Biosystems, Foster City, CA, USA).

    Genome Wide:

    Article Title: Genome-wide expression and response to exposure-based psychological therapy for anxiety disorders
    Article Snippet: Blood RNA was isolated and purified using the PAXgene Blood miRNA Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol using the Qiagen Qiacube. .. RNA quality and integrity were measured using both the Nanodrop 1000 spectrophotometer (Nanodrop 1000, Nanodrop, Wilmington, DE, USA) and the Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA, USA).

    Polymerase Chain Reaction:

    Article Title: Expression of mitochondrial dysfunction-related genes and pathways in paclitaxel-induced peripheral neuropathy in breast cancer survivors
    Article Snippet: Total RNA was isolated using the PAXgene® Blood miRNA Kit (Qiagen, Inc.) using established procedures. .. Prior to library preparation, 600 ng of total RNA was treated with the Illumina Globin-Zero Gold rRNA Removal Kit (Illumina Inc., San Diego, CA) to deplete cytoplasmic ribosomal RNA and human globin mRNA., The globin/ribo depleted RNA was cleaned with Agencourt RNAClean XP (Beckman Coulter, Indianapolis, IN), and the sequencing libraries were prepared with KAPA RNA HyperPrep Kit (Roche Diagnostics Corp., Indianapolis, IN) according to the manufacturer’s protocol.

    Article Title: Recessive Mutations in the α3 (VI) Collagen Gene COL6A3 Cause Early-Onset Isolated Dystonia
    Article Snippet: RNA was isolated from whole blood of three case subjects and a control individual via the PAXgene Blood miRNA kit from QIAGEN. .. Blood total RNA was reverse transcribed to cDNA with the SuperScript First-Strand Synthesis System for RT-PCR (Invitrogen), and the relevant cDNA fragment was amplified with specific primers ( ).

    Agarose Gel Electrophoresis:

    Article Title: MicroRNA Expression Profiles of Whole Blood in Lung Adenocarcinoma
    Article Snippet: Total RNA including small RNA was isolated from blood collected in PAXgene™ Blood RNA tubes using the PAXgene™ Blood miRNA kit (Qiagen®) as per the protocol supplied by the manufacturer. .. Total RNA including small RNA was isolated from blood collected in PAXgene™ Blood RNA tubes using the PAXgene™ Blood miRNA kit (Qiagen®) as per the protocol supplied by the manufacturer.

    Quantitative RT-PCR:

    Article Title: Differential processing of amyloid precursor protein in brain and in peripheral blood leukocytes
    Article Snippet: Paragraph title: 2.6 Quantitative RT-PCR ... 2.5 mL of fresh blood was drawn into PAXgene ™ Blood RNA Tubes (Qiagen, Valencia, CA, USA) and total RNA extracted using PAXgene™ Blood miRNA Kit (Qiagen).

    Variant Assay:

    Article Title: Recessive Mutations in the α3 (VI) Collagen Gene COL6A3 Cause Early-Onset Isolated Dystonia
    Article Snippet: Paragraph title: Splice Variant Analysis ... RNA was isolated from whole blood of three case subjects and a control individual via the PAXgene Blood miRNA kit from QIAGEN.

    Cell Isolation:

    Article Title: Multi-omics monitoring of drug response in rheumatoid arthritis in pursuit of molecular remission
    Article Snippet: Total RNA was isolated using the PAXgene Blood miRNA Kit (763134, Qiagen, Valencia, CA, USA). .. For transcriptome profiling in immune subsets, human whole blood was collected using heparin blood collection tubes (TERUMO, Shibuya, Tokyo, Japan), and immune cell subsets were purified as follows.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Qiagen paxgene mirna blood kit
    Heatmap of 321 <t>miRNAs</t> detected in at least 25% of samples. The X-axis differentiates between the four different blood sampling conditions. The light blue shading indicates blood that is directly (0 min) transferred into <t>PAXgene</t> TM tubes, the middle blue shading blood that is transferred 10min after phlebotomy and the dark blue shading blood that is transferred after 2 h. Blood that was directly collected in PAXgene TM blood RNA tubes is indicated in orange. The 6 individuals are indicated by symbols on top of the dendrogram. While the four samples belonging to one of the six individuals mostly cluster together there was no comparable clustering for any of the four tested blood sampling conditions.
    Paxgene Mirna Blood Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/paxgene mirna blood kit/product/Qiagen
    Average 99 stars, based on 66 article reviews
    Price from $9.99 to $1999.99
    paxgene mirna blood kit - by Bioz Stars, 2019-12
    99/100 stars
      Buy from Supplier

    Image Search Results


    Heatmap of 321 miRNAs detected in at least 25% of samples. The X-axis differentiates between the four different blood sampling conditions. The light blue shading indicates blood that is directly (0 min) transferred into PAXgene TM tubes, the middle blue shading blood that is transferred 10min after phlebotomy and the dark blue shading blood that is transferred after 2 h. Blood that was directly collected in PAXgene TM blood RNA tubes is indicated in orange. The 6 individuals are indicated by symbols on top of the dendrogram. While the four samples belonging to one of the six individuals mostly cluster together there was no comparable clustering for any of the four tested blood sampling conditions.

    Journal: PLoS ONE

    Article Title: Towards Clinical Applications of Blood-Borne miRNA Signatures: The Influence of the Anticoagulant EDTA on miRNA Abundance

    doi: 10.1371/journal.pone.0143321

    Figure Lengend Snippet: Heatmap of 321 miRNAs detected in at least 25% of samples. The X-axis differentiates between the four different blood sampling conditions. The light blue shading indicates blood that is directly (0 min) transferred into PAXgene TM tubes, the middle blue shading blood that is transferred 10min after phlebotomy and the dark blue shading blood that is transferred after 2 h. Blood that was directly collected in PAXgene TM blood RNA tubes is indicated in orange. The 6 individuals are indicated by symbols on top of the dendrogram. While the four samples belonging to one of the six individuals mostly cluster together there was no comparable clustering for any of the four tested blood sampling conditions.

    Article Snippet: Total RNA of all samples was isolated using the PAXgene miRNA blood Kit (Qiagen) and under standardized conditions using the QIAcube (Qiagen).

    Techniques: Sampling

    Microarray present call analysis. A) Average number (Y-axis) and standard deviation of miRNA detected in blood samples of six healthy individuals under four different blood sampling conditions (X-axis). Blood was collected in EDTA blood collection tubes and subsequently transferred into PAXgene TM tubes at three different time points, i.e. directly (0 min), 10 min, and 2 h after phlebotomy. As control blood was also directly collected in PAXgene TM blood RNA tubes. B) Venn diagram of miRNAs that were present in all six individuals under different blood sampling conditions. There were 201 miRNAs detected in all individuals under all conditions. No miRNA was detected both in blood that was directly collected in PAXgene TM blood RNA tubes and in blood that was transferred into PAXgene TM tubes 2 h after phlebotomy without also being detected in blood directly transferred or transferred 10 min after phlebotomy. C) Balloon plot of miRNAs that show a difference in frequency under the different blood sampling conditions. The X-axis shows the minimal number of individuals that are positive for a miRNA under one condition. The Y-axis shows the maximum number of individuals that are positive for a miRNA under one condition. The top left balloon denotes the 806 miRNAs that were not detected in any of the 24 samples (minimum and maximum of 0). The lower right balloon denotes the 201 miRNAs that were detected in all samples (minimum and maximum of 6). The orange shaded area presents 12 miRNAs that show a difference in frequency of at least three under one of the four conditions as compared to the other conditions. The largest difference was found for miR-769-3p indicated in the lower left corner that was positive in 6 samples under the 2h EDTA condition and not in any sample of the PAXgene condition.

    Journal: PLoS ONE

    Article Title: Towards Clinical Applications of Blood-Borne miRNA Signatures: The Influence of the Anticoagulant EDTA on miRNA Abundance

    doi: 10.1371/journal.pone.0143321

    Figure Lengend Snippet: Microarray present call analysis. A) Average number (Y-axis) and standard deviation of miRNA detected in blood samples of six healthy individuals under four different blood sampling conditions (X-axis). Blood was collected in EDTA blood collection tubes and subsequently transferred into PAXgene TM tubes at three different time points, i.e. directly (0 min), 10 min, and 2 h after phlebotomy. As control blood was also directly collected in PAXgene TM blood RNA tubes. B) Venn diagram of miRNAs that were present in all six individuals under different blood sampling conditions. There were 201 miRNAs detected in all individuals under all conditions. No miRNA was detected both in blood that was directly collected in PAXgene TM blood RNA tubes and in blood that was transferred into PAXgene TM tubes 2 h after phlebotomy without also being detected in blood directly transferred or transferred 10 min after phlebotomy. C) Balloon plot of miRNAs that show a difference in frequency under the different blood sampling conditions. The X-axis shows the minimal number of individuals that are positive for a miRNA under one condition. The Y-axis shows the maximum number of individuals that are positive for a miRNA under one condition. The top left balloon denotes the 806 miRNAs that were not detected in any of the 24 samples (minimum and maximum of 0). The lower right balloon denotes the 201 miRNAs that were detected in all samples (minimum and maximum of 6). The orange shaded area presents 12 miRNAs that show a difference in frequency of at least three under one of the four conditions as compared to the other conditions. The largest difference was found for miR-769-3p indicated in the lower left corner that was positive in 6 samples under the 2h EDTA condition and not in any sample of the PAXgene condition.

    Article Snippet: Total RNA of all samples was isolated using the PAXgene miRNA blood Kit (Qiagen) and under standardized conditions using the QIAcube (Qiagen).

    Techniques: Microarray, Standard Deviation, Sampling