cdna library construction  (Qiagen)

 
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    Name:
    PAXgene Blood miRNA Kit
    Description:
    For purification of miRNA and total RNA from whole blood Kit contents Qiagen PAXgene Blood miRNA Kit 50 preps 2 5mL Sample 40L Elution Volume Whole Blood Sample Silica Technology Spin Column Format Manual Processing Ideal for RT PCR and Real time RT PCR cDNA synthesis Microarray Analysis Primer Extension RNase S1 Nuclease Protection For Purification of miRNA and Total RNA from Whole Blood Includes PAXgene Spin Columns PAXgene Shredder Spin Columns Processing Tubes Microcentrifuge Tubes RNase free DNase RNase free Reagents and Buffers Benefits Integrated system for collection stabilization and purification Effective purification of miRNA and total RNA RNA stabilization for up to 3 days at 18 25°C Stabilization for at least 50 months at 20°C or 70°C
    Catalog Number:
    763134
    Price:
    676
    Category:
    PAXgene Blood miRNA Kit
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    Structured Review

    Qiagen cdna library construction
    PAXgene Blood miRNA Kit
    For purification of miRNA and total RNA from whole blood Kit contents Qiagen PAXgene Blood miRNA Kit 50 preps 2 5mL Sample 40L Elution Volume Whole Blood Sample Silica Technology Spin Column Format Manual Processing Ideal for RT PCR and Real time RT PCR cDNA synthesis Microarray Analysis Primer Extension RNase S1 Nuclease Protection For Purification of miRNA and Total RNA from Whole Blood Includes PAXgene Spin Columns PAXgene Shredder Spin Columns Processing Tubes Microcentrifuge Tubes RNase free DNase RNase free Reagents and Buffers Benefits Integrated system for collection stabilization and purification Effective purification of miRNA and total RNA RNA stabilization for up to 3 days at 18 25°C Stabilization for at least 50 months at 20°C or 70°C
    https://www.bioz.com/result/cdna library construction/product/Qiagen
    Average 92 stars, based on 22652 article reviews
    Price from $9.99 to $1999.99
    cdna library construction - by Bioz Stars, 2020-08
    92/100 stars

    Images

    1) Product Images from "A benchmark of hemoglobin blocking during library preparation for mRNA-Sequencing of human blood samples"

    Article Title: A benchmark of hemoglobin blocking during library preparation for mRNA-Sequencing of human blood samples

    Journal: Scientific Reports

    doi: 10.1038/s41598-020-62637-0

    Study design to test the performance of Lexogen’s commercially available globin-cDNA blocking method that was applied during NGS RNA library preparation (thus preserving RNA quality of the original sample). Total RNA was isolated from whole blood samples of 91 healthy donors (see Methods). For data set 1, two libraries were prepared from all 91 RNA isolates, i.e. one with Lexogen’s Globin Block (GB) and one without (noGB), followed by sequencing on the HiSeq2500 instrument. After quality control (QC), 88 pairs of GB/noGB samples were available for benchmark purposes. For data set 2, 8 GB and noGB samples from Data Set 1 were re-sequenced on a HiSeq4000 to assess technical reproducibility for GB samples.
    Figure Legend Snippet: Study design to test the performance of Lexogen’s commercially available globin-cDNA blocking method that was applied during NGS RNA library preparation (thus preserving RNA quality of the original sample). Total RNA was isolated from whole blood samples of 91 healthy donors (see Methods). For data set 1, two libraries were prepared from all 91 RNA isolates, i.e. one with Lexogen’s Globin Block (GB) and one without (noGB), followed by sequencing on the HiSeq2500 instrument. After quality control (QC), 88 pairs of GB/noGB samples were available for benchmark purposes. For data set 2, 8 GB and noGB samples from Data Set 1 were re-sequenced on a HiSeq4000 to assess technical reproducibility for GB samples.

    Techniques Used: Blocking Assay, Next-Generation Sequencing, Preserving, Isolation, Sequencing

    2) Product Images from "Next generation MicroRNA sequencing to identify coronary artery disease patients at risk of recurrent myocardial infarction"

    Article Title: Next generation MicroRNA sequencing to identify coronary artery disease patients at risk of recurrent myocardial infarction

    Journal: Atherosclerosis

    doi: 10.1016/j.atherosclerosis.2018.09.021

    Heat map of the expression profiles of the miRNA array analysis of 22 coronary artery disease patients with recurrent coronary events (blue) and 26 patients with no recurrent thrombotic coronary events (red). Subjects with stent thrombosis during follow up are identified. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Figure Legend Snippet: Heat map of the expression profiles of the miRNA array analysis of 22 coronary artery disease patients with recurrent coronary events (blue) and 26 patients with no recurrent thrombotic coronary events (red). Subjects with stent thrombosis during follow up are identified. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Techniques Used: Expressing

    Expression pattern of circulating distinct miRNA levels in blood samples of patients undergoing cardiac catheterization. Next generation miRNA sequence analysis of miRNA of whole blood samples of subjects with coronary disease and recurrent events and subjects with coronary disease and no recurrent events with false discovery rate of
    Figure Legend Snippet: Expression pattern of circulating distinct miRNA levels in blood samples of patients undergoing cardiac catheterization. Next generation miRNA sequence analysis of miRNA of whole blood samples of subjects with coronary disease and recurrent events and subjects with coronary disease and no recurrent events with false discovery rate of

    Techniques Used: Expressing, Sequencing

    Heat map of the expression profiles of the miRNA array analysis of 48 coronary artery disease patients (blue) and 24 controls without coronary disease on angiography (yellow). Differences in miRNA expression in CAD patients with recurrent thrombotic events vs. CAD patients with no recurrent events. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Figure Legend Snippet: Heat map of the expression profiles of the miRNA array analysis of 48 coronary artery disease patients (blue) and 24 controls without coronary disease on angiography (yellow). Differences in miRNA expression in CAD patients with recurrent thrombotic events vs. CAD patients with no recurrent events. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Techniques Used: Expressing

    3) Product Images from "A benchmark of hemoglobin blocking during library preparation for mRNA-Sequencing of human blood samples"

    Article Title: A benchmark of hemoglobin blocking during library preparation for mRNA-Sequencing of human blood samples

    Journal: Scientific Reports

    doi: 10.1038/s41598-020-62637-0

    Study design to test the performance of Lexogen’s commercially available globin-cDNA blocking method that was applied during NGS RNA library preparation (thus preserving RNA quality of the original sample). Total RNA was isolated from whole blood samples of 91 healthy donors (see Methods). For data set 1, two libraries were prepared from all 91 RNA isolates, i.e. one with Lexogen’s Globin Block (GB) and one without (noGB), followed by sequencing on the HiSeq2500 instrument. After quality control (QC), 88 pairs of GB/noGB samples were available for benchmark purposes. For data set 2, 8 GB and noGB samples from Data Set 1 were re-sequenced on a HiSeq4000 to assess technical reproducibility for GB samples.
    Figure Legend Snippet: Study design to test the performance of Lexogen’s commercially available globin-cDNA blocking method that was applied during NGS RNA library preparation (thus preserving RNA quality of the original sample). Total RNA was isolated from whole blood samples of 91 healthy donors (see Methods). For data set 1, two libraries were prepared from all 91 RNA isolates, i.e. one with Lexogen’s Globin Block (GB) and one without (noGB), followed by sequencing on the HiSeq2500 instrument. After quality control (QC), 88 pairs of GB/noGB samples were available for benchmark purposes. For data set 2, 8 GB and noGB samples from Data Set 1 were re-sequenced on a HiSeq4000 to assess technical reproducibility for GB samples.

    Techniques Used: Blocking Assay, Next-Generation Sequencing, Preserving, Isolation, Sequencing

    4) Product Images from "Towards Clinical Applications of Blood-Borne miRNA Signatures: The Influence of the Anticoagulant EDTA on miRNA Abundance"

    Article Title: Towards Clinical Applications of Blood-Borne miRNA Signatures: The Influence of the Anticoagulant EDTA on miRNA Abundance

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0143321

    Heatmap of 321 miRNAs detected in at least 25% of samples. The X-axis differentiates between the four different blood sampling conditions. The light blue shading indicates blood that is directly (0 min) transferred into PAXgene TM tubes, the middle blue shading blood that is transferred 10min after phlebotomy and the dark blue shading blood that is transferred after 2 h. Blood that was directly collected in PAXgene TM blood RNA tubes is indicated in orange. The 6 individuals are indicated by symbols on top of the dendrogram. While the four samples belonging to one of the six individuals mostly cluster together there was no comparable clustering for any of the four tested blood sampling conditions.
    Figure Legend Snippet: Heatmap of 321 miRNAs detected in at least 25% of samples. The X-axis differentiates between the four different blood sampling conditions. The light blue shading indicates blood that is directly (0 min) transferred into PAXgene TM tubes, the middle blue shading blood that is transferred 10min after phlebotomy and the dark blue shading blood that is transferred after 2 h. Blood that was directly collected in PAXgene TM blood RNA tubes is indicated in orange. The 6 individuals are indicated by symbols on top of the dendrogram. While the four samples belonging to one of the six individuals mostly cluster together there was no comparable clustering for any of the four tested blood sampling conditions.

    Techniques Used: Sampling

    Microarray present call analysis. A) Average number (Y-axis) and standard deviation of miRNA detected in blood samples of six healthy individuals under four different blood sampling conditions (X-axis). Blood was collected in EDTA blood collection tubes and subsequently transferred into PAXgene TM tubes at three different time points, i.e. directly (0 min), 10 min, and 2 h after phlebotomy. As control blood was also directly collected in PAXgene TM blood RNA tubes. B) Venn diagram of miRNAs that were present in all six individuals under different blood sampling conditions. There were 201 miRNAs detected in all individuals under all conditions. No miRNA was detected both in blood that was directly collected in PAXgene TM blood RNA tubes and in blood that was transferred into PAXgene TM tubes 2 h after phlebotomy without also being detected in blood directly transferred or transferred 10 min after phlebotomy. C) Balloon plot of miRNAs that show a difference in frequency under the different blood sampling conditions. The X-axis shows the minimal number of individuals that are positive for a miRNA under one condition. The Y-axis shows the maximum number of individuals that are positive for a miRNA under one condition. The top left balloon denotes the 806 miRNAs that were not detected in any of the 24 samples (minimum and maximum of 0). The lower right balloon denotes the 201 miRNAs that were detected in all samples (minimum and maximum of 6). The orange shaded area presents 12 miRNAs that show a difference in frequency of at least three under one of the four conditions as compared to the other conditions. The largest difference was found for miR-769-3p indicated in the lower left corner that was positive in 6 samples under the 2h EDTA condition and not in any sample of the PAXgene condition.
    Figure Legend Snippet: Microarray present call analysis. A) Average number (Y-axis) and standard deviation of miRNA detected in blood samples of six healthy individuals under four different blood sampling conditions (X-axis). Blood was collected in EDTA blood collection tubes and subsequently transferred into PAXgene TM tubes at three different time points, i.e. directly (0 min), 10 min, and 2 h after phlebotomy. As control blood was also directly collected in PAXgene TM blood RNA tubes. B) Venn diagram of miRNAs that were present in all six individuals under different blood sampling conditions. There were 201 miRNAs detected in all individuals under all conditions. No miRNA was detected both in blood that was directly collected in PAXgene TM blood RNA tubes and in blood that was transferred into PAXgene TM tubes 2 h after phlebotomy without also being detected in blood directly transferred or transferred 10 min after phlebotomy. C) Balloon plot of miRNAs that show a difference in frequency under the different blood sampling conditions. The X-axis shows the minimal number of individuals that are positive for a miRNA under one condition. The Y-axis shows the maximum number of individuals that are positive for a miRNA under one condition. The top left balloon denotes the 806 miRNAs that were not detected in any of the 24 samples (minimum and maximum of 0). The lower right balloon denotes the 201 miRNAs that were detected in all samples (minimum and maximum of 6). The orange shaded area presents 12 miRNAs that show a difference in frequency of at least three under one of the four conditions as compared to the other conditions. The largest difference was found for miR-769-3p indicated in the lower left corner that was positive in 6 samples under the 2h EDTA condition and not in any sample of the PAXgene condition.

    Techniques Used: Microarray, Standard Deviation, Sampling

    5) Product Images from "Next generation MicroRNA sequencing to identify coronary artery disease patients at risk of recurrent myocardial infarction"

    Article Title: Next generation MicroRNA sequencing to identify coronary artery disease patients at risk of recurrent myocardial infarction

    Journal: Atherosclerosis

    doi: 10.1016/j.atherosclerosis.2018.09.021

    Heat map of the expression profiles of the miRNA array analysis of 22 coronary artery disease patients with recurrent coronary events (blue) and 26 patients with no recurrent thrombotic coronary events (red). Subjects with stent thrombosis during follow up are identified. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Figure Legend Snippet: Heat map of the expression profiles of the miRNA array analysis of 22 coronary artery disease patients with recurrent coronary events (blue) and 26 patients with no recurrent thrombotic coronary events (red). Subjects with stent thrombosis during follow up are identified. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Techniques Used: Expressing

    Expression pattern of circulating distinct miRNA levels in blood samples of patients undergoing cardiac catheterization. Next generation miRNA sequence analysis of miRNA of whole blood samples of subjects with coronary disease and recurrent events and subjects with coronary disease and no recurrent events with false discovery rate of
    Figure Legend Snippet: Expression pattern of circulating distinct miRNA levels in blood samples of patients undergoing cardiac catheterization. Next generation miRNA sequence analysis of miRNA of whole blood samples of subjects with coronary disease and recurrent events and subjects with coronary disease and no recurrent events with false discovery rate of

    Techniques Used: Expressing, Sequencing

    Heat map of the expression profiles of the miRNA array analysis of 48 coronary artery disease patients (blue) and 24 controls without coronary disease on angiography (yellow). Differences in miRNA expression in CAD patients with recurrent thrombotic events vs. CAD patients with no recurrent events. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Figure Legend Snippet: Heat map of the expression profiles of the miRNA array analysis of 48 coronary artery disease patients (blue) and 24 controls without coronary disease on angiography (yellow). Differences in miRNA expression in CAD patients with recurrent thrombotic events vs. CAD patients with no recurrent events. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Techniques Used: Expressing

    Related Articles

    Isolation:

    Article Title: Towards Clinical Applications of Blood-Borne miRNA Signatures: The Influence of the Anticoagulant EDTA on miRNA Abundance
    Article Snippet: .. RNA isolation Total RNA of all samples was isolated using the PAXgene miRNA blood Kit (Qiagen) and under standardized conditions using the QIAcube (Qiagen). ..

    Article Title: The sbv IMPROVER Systems Toxicology Computational Challenge: Identification of Human and Species-Independent Blood Response Markers as Predictors of Smoking Exposure and Cessation Status
    Article Snippet: .. For the clinical studies, total RNA was isolated using a PAXgene™ Blood miRNA Kit (catalog number 763134; Qiagen, Venlo, The Netherlands) according to the manufacturer’s instructions. .. For the in vivo mouse studies, total RNA was isolated using a RNeasy Protect Animal Blood Kit (catalog number 73224; Qiagen, Venlo, The Netherlands) according to the manufacturer’s instructions.

    Article Title: Minority Stress and Leukocyte Gene Expression in Sexual Minority Men Living with Treated HIV Infection
    Article Snippet: .. Total RNA was isolated from samples in PAXgene tubes using the PAXgene blood miRNA kit (Qiagen). .. Library preparation and sequencing was done by a Core Facility, the Vincent J. Coates Genomics Sequencing Laboratory at the University of California, Berkeley.

    Spectrophotometry:

    Article Title: Using Domestic and Free-Ranging Arctic Canid Models for Environmental Molecular Toxicology Research
    Article Snippet: .. RNA was extracted using PaxGene Blood miRNA Kit (Qiagen) and quantity and integrity determined using an Agilent 2100 Bioanalyzer (Agilent Technologies) or a Nano-Drop ND-1000 Spectrophotometer (Thermo Fisher Scientific). .. RNA integrity within the cell is dependent on a complex series of responses that are set in motion in response to insult (prior to or concurrent with sampling), including the critical interval between the time of collection and time of tissue preservation (e.g., stabilization of mRNA).

    Purification:

    Article Title: Circulating ADAM17 Level Reflects Disease Activity in Proteinase-3 ANCA-Associated Vasculitis
    Article Snippet: .. Total RNA and miRNA from whole blood stabilized in PAXgene Blood RNA tubes were purified using the PAXgene Blood miRNA Kit according to the manufacturer’s instructions (Qiagen). .. Gene-specific oligonucleotides for ADAM17, ADAM10, and TIMP3 and miScript primers for miRNA-643 were obtained from Qiagen (QuantiTect Primer Assay) with the following corresponding ordering numbers: ADAM17 (QT00055580), ADAM10 (QT00032641), TIMP3 (QT00046382), and miRNA-634 (MS00005201).

    Microarray:

    Article Title: A Genome-Wide Integrative Association Study of DNA Methylation and Gene Expression Data and Later Life Cognitive Functioning in Monozygotic Twins
    Article Snippet: .. Genome-Wide Transcriptome Data Whole blood was collected in PAXgene Blood RNA Tubes (PreAnalytiX GmbH, Hombrechtikon, Switzerland), total RNA was extracted by the PAXgene Blood miRNA kit (QIAGEN), and gene expression was examined by the Agilent SurePrint G3 Human GE 8 × 60K Microarray (Agilent Technologies). .. Sample labeling and array hybridization were performed by the ‘Two-Color Microarray-Based Gene Expression Analysis – Low Input Quick Amp Labeling’ protocol (Agilent Technologies); samples were labeled with Cy5 and the reference (a pool of 16 samples) was labeled with Cy3.

    Expressing:

    Article Title: Associations between Serum Sex Hormone Concentrations and Whole Blood Gene Expression Profiles in the General Population
    Article Snippet: .. Gene expression data RNA was prepared from whole blood collected and stored in PAXgene Blood RNA Tubes (BD, Heidelberg, Germany) using the PAXgene Blood miRNA Kit (Qiagen, Hilden, Germany). .. Isolation of RNA was performed using a QIAcube according to protocols provided by the manufacturer Qiagen.

    Article Title: A Genome-Wide Integrative Association Study of DNA Methylation and Gene Expression Data and Later Life Cognitive Functioning in Monozygotic Twins
    Article Snippet: .. Genome-Wide Transcriptome Data Whole blood was collected in PAXgene Blood RNA Tubes (PreAnalytiX GmbH, Hombrechtikon, Switzerland), total RNA was extracted by the PAXgene Blood miRNA kit (QIAGEN), and gene expression was examined by the Agilent SurePrint G3 Human GE 8 × 60K Microarray (Agilent Technologies). .. Sample labeling and array hybridization were performed by the ‘Two-Color Microarray-Based Gene Expression Analysis – Low Input Quick Amp Labeling’ protocol (Agilent Technologies); samples were labeled with Cy5 and the reference (a pool of 16 samples) was labeled with Cy3.

    Genome Wide:

    Article Title: A Genome-Wide Integrative Association Study of DNA Methylation and Gene Expression Data and Later Life Cognitive Functioning in Monozygotic Twins
    Article Snippet: .. Genome-Wide Transcriptome Data Whole blood was collected in PAXgene Blood RNA Tubes (PreAnalytiX GmbH, Hombrechtikon, Switzerland), total RNA was extracted by the PAXgene Blood miRNA kit (QIAGEN), and gene expression was examined by the Agilent SurePrint G3 Human GE 8 × 60K Microarray (Agilent Technologies). .. Sample labeling and array hybridization were performed by the ‘Two-Color Microarray-Based Gene Expression Analysis – Low Input Quick Amp Labeling’ protocol (Agilent Technologies); samples were labeled with Cy5 and the reference (a pool of 16 samples) was labeled with Cy3.

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    Qiagen paxgene mirna blood kit
    Heatmap of 321 <t>miRNAs</t> detected in at least 25% of samples. The X-axis differentiates between the four different blood sampling conditions. The light blue shading indicates blood that is directly (0 min) transferred into <t>PAXgene</t> TM tubes, the middle blue shading blood that is transferred 10min after phlebotomy and the dark blue shading blood that is transferred after 2 h. Blood that was directly collected in PAXgene TM blood RNA tubes is indicated in orange. The 6 individuals are indicated by symbols on top of the dendrogram. While the four samples belonging to one of the six individuals mostly cluster together there was no comparable clustering for any of the four tested blood sampling conditions.
    Paxgene Mirna Blood Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/paxgene mirna blood kit/product/Qiagen
    Average 99 stars, based on 131 article reviews
    Price from $9.99 to $1999.99
    paxgene mirna blood kit - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    Heatmap of 321 miRNAs detected in at least 25% of samples. The X-axis differentiates between the four different blood sampling conditions. The light blue shading indicates blood that is directly (0 min) transferred into PAXgene TM tubes, the middle blue shading blood that is transferred 10min after phlebotomy and the dark blue shading blood that is transferred after 2 h. Blood that was directly collected in PAXgene TM blood RNA tubes is indicated in orange. The 6 individuals are indicated by symbols on top of the dendrogram. While the four samples belonging to one of the six individuals mostly cluster together there was no comparable clustering for any of the four tested blood sampling conditions.

    Journal: PLoS ONE

    Article Title: Towards Clinical Applications of Blood-Borne miRNA Signatures: The Influence of the Anticoagulant EDTA on miRNA Abundance

    doi: 10.1371/journal.pone.0143321

    Figure Lengend Snippet: Heatmap of 321 miRNAs detected in at least 25% of samples. The X-axis differentiates between the four different blood sampling conditions. The light blue shading indicates blood that is directly (0 min) transferred into PAXgene TM tubes, the middle blue shading blood that is transferred 10min after phlebotomy and the dark blue shading blood that is transferred after 2 h. Blood that was directly collected in PAXgene TM blood RNA tubes is indicated in orange. The 6 individuals are indicated by symbols on top of the dendrogram. While the four samples belonging to one of the six individuals mostly cluster together there was no comparable clustering for any of the four tested blood sampling conditions.

    Article Snippet: RNA isolation Total RNA of all samples was isolated using the PAXgene miRNA blood Kit (Qiagen) and under standardized conditions using the QIAcube (Qiagen).

    Techniques: Sampling

    Microarray present call analysis. A) Average number (Y-axis) and standard deviation of miRNA detected in blood samples of six healthy individuals under four different blood sampling conditions (X-axis). Blood was collected in EDTA blood collection tubes and subsequently transferred into PAXgene TM tubes at three different time points, i.e. directly (0 min), 10 min, and 2 h after phlebotomy. As control blood was also directly collected in PAXgene TM blood RNA tubes. B) Venn diagram of miRNAs that were present in all six individuals under different blood sampling conditions. There were 201 miRNAs detected in all individuals under all conditions. No miRNA was detected both in blood that was directly collected in PAXgene TM blood RNA tubes and in blood that was transferred into PAXgene TM tubes 2 h after phlebotomy without also being detected in blood directly transferred or transferred 10 min after phlebotomy. C) Balloon plot of miRNAs that show a difference in frequency under the different blood sampling conditions. The X-axis shows the minimal number of individuals that are positive for a miRNA under one condition. The Y-axis shows the maximum number of individuals that are positive for a miRNA under one condition. The top left balloon denotes the 806 miRNAs that were not detected in any of the 24 samples (minimum and maximum of 0). The lower right balloon denotes the 201 miRNAs that were detected in all samples (minimum and maximum of 6). The orange shaded area presents 12 miRNAs that show a difference in frequency of at least three under one of the four conditions as compared to the other conditions. The largest difference was found for miR-769-3p indicated in the lower left corner that was positive in 6 samples under the 2h EDTA condition and not in any sample of the PAXgene condition.

    Journal: PLoS ONE

    Article Title: Towards Clinical Applications of Blood-Borne miRNA Signatures: The Influence of the Anticoagulant EDTA on miRNA Abundance

    doi: 10.1371/journal.pone.0143321

    Figure Lengend Snippet: Microarray present call analysis. A) Average number (Y-axis) and standard deviation of miRNA detected in blood samples of six healthy individuals under four different blood sampling conditions (X-axis). Blood was collected in EDTA blood collection tubes and subsequently transferred into PAXgene TM tubes at three different time points, i.e. directly (0 min), 10 min, and 2 h after phlebotomy. As control blood was also directly collected in PAXgene TM blood RNA tubes. B) Venn diagram of miRNAs that were present in all six individuals under different blood sampling conditions. There were 201 miRNAs detected in all individuals under all conditions. No miRNA was detected both in blood that was directly collected in PAXgene TM blood RNA tubes and in blood that was transferred into PAXgene TM tubes 2 h after phlebotomy without also being detected in blood directly transferred or transferred 10 min after phlebotomy. C) Balloon plot of miRNAs that show a difference in frequency under the different blood sampling conditions. The X-axis shows the minimal number of individuals that are positive for a miRNA under one condition. The Y-axis shows the maximum number of individuals that are positive for a miRNA under one condition. The top left balloon denotes the 806 miRNAs that were not detected in any of the 24 samples (minimum and maximum of 0). The lower right balloon denotes the 201 miRNAs that were detected in all samples (minimum and maximum of 6). The orange shaded area presents 12 miRNAs that show a difference in frequency of at least three under one of the four conditions as compared to the other conditions. The largest difference was found for miR-769-3p indicated in the lower left corner that was positive in 6 samples under the 2h EDTA condition and not in any sample of the PAXgene condition.

    Article Snippet: RNA isolation Total RNA of all samples was isolated using the PAXgene miRNA blood Kit (Qiagen) and under standardized conditions using the QIAcube (Qiagen).

    Techniques: Microarray, Standard Deviation, Sampling