rneasy plant mini kit (Qiagen)

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RNeasy Plant Mini Kit

Description:

For purification of total RNA from plants and fungi Kit contents Qiagen RNeasy Plant Mini Kit 20 preps 10 to 100mg Sample 30 to 100L Elution Volume Plant Sample Total RNA Purification Spin Column Format Silica Technology Ideal for Northern Dot and Slot Blotting End point RT PCR Quantitative Real time RT PCR Array Analysis Includes 20 RNeasy Mini Spin Columns 20 QIAshredder Mini Spin Columns Collection Tubes 1 5mL and 2mL RNase free Reagents and Buffers Benefits High quality total RNA in 30 minutes No phenol chloroform extraction No CsCl gradients no LiCl or ethanol precipitation Excellent recovery of RNA Ready to use RNA for any downstream applicatio

Catalog Number:

74903

Price:

155

Category:

RNeasy Plant Mini Kit

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Structured Review

Qiagen rneasy plant mini kit

rneasy plant mini kit - by Bioz Stars, 2021-04

99/100 stars

Images

1) Product Images from "Multiplex Detection of Aspergillus fumigatus Mycoviruses"

Article Title: Multiplex Detection of Aspergillus fumigatus Mycoviruses

Journal: Viruses

doi: 10.3390/v10050247

Conventional PCR to check amplicon size prior to multiplex PCR. Amplicon sizes were checked on two percent agarose gel prior to performing multiplex PCR for A. fumigatus dsRNA mycoviruses. AfuCV, AfuPV-1 and AfuTmV-1 dsRNAs were extracted using LiCl extraction (Lanes 2, 4 and 6, respectively) and RNeasy Plant Mini kit (Lanes 3, 5 and 7, respectively) and used as templates for amplification with AfuCV, AfuPV-1 and AfuTmV-1 primers (2–3, 4–5, 6–7, respectively). Hyperladder-I was used as a marker to estimate the size of the amplicons (Lane 1).

Figure Legend Snippet: Conventional PCR to check amplicon size prior to multiplex PCR. Amplicon sizes were checked on two percent agarose gel prior to performing multiplex PCR for A. fumigatus dsRNA mycoviruses. AfuCV, AfuPV-1 and AfuTmV-1 dsRNAs were extracted using LiCl extraction (Lanes 2, 4 and 6, respectively) and RNeasy Plant Mini kit (Lanes 3, 5 and 7, respectively) and used as templates for amplification with AfuCV, AfuPV-1 and AfuTmV-1 primers (2–3, 4–5, 6–7, respectively). Hyperladder-I was used as a marker to estimate the size of the amplicons (Lane 1).

Techniques Used: Polymerase Chain Reaction, Amplification, Multiplex Assay, Agarose Gel Electrophoresis, Marker

Isolation:

Article Title: Identification of a putative polyketide synthase gene involved in usnic acid biosynthesis in the lichen Nephromopsis pallescens
Article Snippet: For lichen-forming fungi samples in which usnic acid production was confirmed through LCMS-IT-TOF analyses, the frozen mycelia were ground into powder for RNA extraction. .. Total RNA was isolated using the RNeasy Plant mini Kit (Qiagen, Hilden, Germany). .. The concentration and quality was examined using a NanoDrop 2000 spectrophotometer (Thermo Scientific, Hudson, NH, USA) and 0.8% agrose gel electrophoresis.

Article Title: A Near-Complete Haplotype-Phased Genome of the Dikaryotic Wheat Stripe Rust Fungus Puccinia striiformis f. sp. tritici Reveals High Interhaplotype Diversity
Article Snippet: RNA for all samples was isolated as follows. .. Total RNA was isolated using the Qiagen plant RNeasy kit following the manufacturer’s instructions. .. Initial RNA quality and purity checks were performed on a NanoDrop ND-1000 UV-vis spectrophotometer.

Article Title: Arabidopsis PCaP2 Plays an Important Role in Chilling Tolerance and ABA Response by Activating CBF- and SnRK2-Mediated Transcriptional Regulatory Network
Article Snippet: .. Total RNA was isolated using an RNeasy Plant Mini Kit (Qiagen), and treated with RNase-Free DNase (Qiagen) to remove DNA contamination by the manufacturer’s instructions. cDNA was synthesized using 1 μg total RNA by oligo- (dT) 20-primed reverse transcription using the Omniscript RT Kit (Qiagen). .. The total reaction volume was 20 and 1 μL of the reaction mixture was subjected to the quantitative real time PCR reaction.

Polymerase Chain Reaction:

Article Title: Multiplex Detection of Aspergillus fumigatus Mycoviruses
Article Snippet: The multiplex RT-PCR amplification assay was used to detect known and characterized A. fumigatus mycoviruses, namely AfuCV, AfuPV-1 and AfuTmV-1. .. The effect of the dsRNA extraction method on the efficiency of PCR was tested using LiCl extraction and the RNeasy Plant Mini kit (Qiagen). ..

Synthesized:

Incubation:

Article Title: Evidence of non-tandemly repeated rDNAs and their intragenomic heterogeneity in Rhizophagus irregularis
Article Snippet: The expression levels of the rDNA paralogs were examined with modified Illumina sequencing of R. irregularis spores and hyphae. .. Total RNA was extracted with an RNeasy Plant Mini kit (Qiagen) after the incubation of the MYCORISE® spores in a minimum nutrient medium for 7 days. .. An Illumina RNA-Seq library was constructed with a TruSeq Stranded mRNA Library prep kit (Illumina).