rneasy minelute cleanup kit  (Qiagen)


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    Name:
    MinElute Reaction Cleanup Kit
    Description:

    Catalog Number:
    28204
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    Score:
    85
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    Qiagen rneasy minelute cleanup kit

    https://www.bioz.com/result/rneasy minelute cleanup kit/product/Qiagen
    Average 99 stars, based on 907 article reviews
    Price from $9.99 to $1999.99
    rneasy minelute cleanup kit - by Bioz Stars, 2019-12
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    Related Articles

    Transduction:

    Article Title: DNA Methylation and Histone Modifications Are the Molecular Lock in Lentivirally Transduced Hematopoietic Progenitor Cells
    Article Snippet: Methylated pL/CMV/GFP (1 μ g) (served as the positive control) and the genomic DNA (1 μ g) from the transduced cells harvested at day 2 and day 7 after transduction were subjected to restriction enzyme digestion prior to bisulfite conversion. .. The restriction digested DNA was purified from the enzymatic reaction by using MinElute Reaction Cleanup Kit (Qiagen).

    Amplification:

    Article Title: Human non-small cell lung cancer expresses putative cancer stem cell markers and exhibits the transcriptomic profile of multipotent cells
    Article Snippet: Total RNA (1.5 μg) was amplified using the Applause™ WT-Amp ST System (Nugen Technologies, Inc., San Carlos, USA) following the manufacturer’s protocol. .. The seven step amplification process produced ST-cDNA, which was further purified using the MinElute Reaction Cleanup Kit (Qiagen). .. The yield and purity of the purified ST-cDNA were measured using the Nanodrop® ND1000 spectrophotometer.

    Article Title: Peripheral T cell cytotoxicity predicts T cell function in the tumor microenvironment
    Article Snippet: After removal of the adaptor and primer with the MinElute Reaction Cleanup kit (Qiagen), PCR was performed with KAPA HiFi DNA Polymerase (Kapa Biosystems) using TCR α-chain constant region-specific (CA1) or TCR β-chain constant region-specific primers (CB1) and P20EA (Supplementary Table ). .. Second PCR was performed with CA2 or CB2 and P20EA primers using the same PCR conditions.

    Article Title: Activation of defence pathways in Scots pine bark after feeding by pine weevil (Hylobius abietis)
    Article Snippet: The treated RNA was then purified using RNeasy MinElute Cleanup Kit according to the manufacturer's instruction (Qiagen, Finland) and eluted in 14 μl nuclease-free water. .. The treated RNA was then purified using RNeasy MinElute Cleanup Kit according to the manufacturer's instruction (Qiagen, Finland) and eluted in 14 μl nuclease-free water.

    Stable Transfection:

    Article Title: CircFAT1 sponges miR-375 to promote the expression of Yes-associated protein 1 in osteosarcoma cells
    Article Snippet: We first constructed the lentivirus vectors harbouring vector or shcircFAT1, and established two stable cell lines of HOS. .. After treating with proteinase K buffer, the immunoprecipitated RNAs were extracted by RNeasy MinElute Cleanup Kit (Qiagen) and reversely transcripted using Prime- Script RT Master Mix (TaKaRa).

    Synthesized:

    Article Title: Peripheral T cell cytotoxicity predicts T cell function in the tumor microenvironment
    Article Snippet: After cDNA synthesis, double-strand (ds)-cDNA was synthesized with E . coli DNA polymerase I (Invitrogen), E . coli DNA Ligase (Invitrogen), and RNase H (Invitrogen). ds-cDNAs were blunted with T4 DNA polymerase (Invitrogen). .. After removal of the adaptor and primer with the MinElute Reaction Cleanup kit (Qiagen), PCR was performed with KAPA HiFi DNA Polymerase (Kapa Biosystems) using TCR α-chain constant region-specific (CA1) or TCR β-chain constant region-specific primers (CB1) and P20EA (Supplementary Table ).

    Picogreen Assay:

    Article Title: Evolutionary diversity and novelty of DNA repair genes in asexual Bdelloid rotifers
    Article Snippet: TRIzol Reagent, TURBO DNA-free Kit, Quant-iT RiboGreen RNA Assay, and Quant-iT PicoGreen dsDNA Assay Kit were from Thermo Fisher Scientific. .. Linear acrylamide was from AMRESCO, Solon, OH and the RNeasy MinElute Cleanup Kit was from Qiagen, Germantown, MD.

    Construct:

    Article Title: CircFAT1 sponges miR-375 to promote the expression of Yes-associated protein 1 in osteosarcoma cells
    Article Snippet: We first constructed the lentivirus vectors harbouring vector or shcircFAT1, and established two stable cell lines of HOS. .. After treating with proteinase K buffer, the immunoprecipitated RNAs were extracted by RNeasy MinElute Cleanup Kit (Qiagen) and reversely transcripted using Prime- Script RT Master Mix (TaKaRa).

    Concentration Assay:

    Article Title: Human non-small cell lung cancer expresses putative cancer stem cell markers and exhibits the transcriptomic profile of multipotent cells
    Article Snippet: The seven step amplification process produced ST-cDNA, which was further purified using the MinElute Reaction Cleanup Kit (Qiagen). .. The yield and purity of the purified ST-cDNA were measured using the Nanodrop® ND1000 spectrophotometer.

    Article Title: DNA Methylation and Histone Modifications Are the Molecular Lock in Lentivirally Transduced Hematopoietic Progenitor Cells
    Article Snippet: The concentration and the purity of DNA were determined by Nanodrop 2000 (Thermo Scientific, Wilmington, DE). .. The restriction digested DNA was purified from the enzymatic reaction by using MinElute Reaction Cleanup Kit (Qiagen).

    Article Title: Innate immune activation in neonatal tracheal aspirates suggests endotoxin-driven inflammation
    Article Snippet: RNA was isolated by RNA Protect Cell Mini and RNeasy MinElute Cleanup Kits (QIAGEN) per the manufacturer's instructions. .. RNA was isolated by RNA Protect Cell Mini and RNeasy MinElute Cleanup Kits (QIAGEN) per the manufacturer's instructions.

    Real-time Polymerase Chain Reaction:

    Article Title: Brain region-specific gene expression profiles in freshly isolated rat microglia
    Article Snippet: Paragraph title: RNA Isolation, cDNA Synthesis and Quantitative Real-Time PCR ... Total RNA was further purified using the RNeasy MinElute Cleanup kit (Qiagen).

    Article Title: Evolutionary diversity and novelty of DNA repair genes in asexual Bdelloid rotifers
    Article Snippet: Linear acrylamide was from AMRESCO, Solon, OH and the RNeasy MinElute Cleanup Kit was from Qiagen, Germantown, MD. .. Linear acrylamide was from AMRESCO, Solon, OH and the RNeasy MinElute Cleanup Kit was from Qiagen, Germantown, MD.

    Article Title: Maximum Diastolic Potential of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes Depends Critically on IKr
    Article Snippet: qPCR analysis was performed with the ABI Prism 7000 Sequence Detection System (Applied Biosystems, Foster City, CA, USA). .. Total RNA was extracted with RNAeasy MinElute Cleanup Kit (Qiagen, CA).

    Microarray:

    Article Title: Activation of defence pathways in Scots pine bark after feeding by pine weevil (Hylobius abietis)
    Article Snippet: Paragraph title: cDNA preparation for microarray experiments ... The treated RNA was then purified using RNeasy MinElute Cleanup Kit according to the manufacturer's instruction (Qiagen, Finland) and eluted in 14 μl nuclease-free water.

    Article Title: Developmental-stage-dependent transcriptional response to leukaemic oncogene expression
    Article Snippet: Paragraph title: Microarray and data analysis ... The mixture was loaded onto a RNeasy Minelute column (Qiagen, 74204) and purified as per the manufacturer's instructions.

    Incubation:

    Article Title: Ancient nuclear genomes enable repatriation of Indigenous human remains
    Article Snippet: The supernatant was combined with 10× volume of a modified binding buffer [500 ml of buffer PB (phosphate buffer; Qiagen), 1:250 pH indicator I, 15 ml of 3 M NaOAC (pH 5.2), and 1.25 ml of NaCl]. .. Extractions were purified using the MinElute Reaction Cleanup Kit (Qiagen) following the manufacturer’s protocol and eluted using 100 μl of buffer EB (elution buffer; Qiagen) after incubation for 10 min at 37°C. .. Double-stranded Illumina DNA libraries were built according to the methods previously described ( , , ), with some minor modifications in the Taq polymerase used for amplification.

    Article Title: DNA Methylation and Histone Modifications Are the Molecular Lock in Lentivirally Transduced Hematopoietic Progenitor Cells
    Article Snippet: All the samples were digested with 10 U of EcoR1 and AvaI in 1x NE buffer 4 in a total reaction volume of 50 μ L. The reaction mixture was incubated at 37°C for 1 hour. .. The restriction digested DNA was purified from the enzymatic reaction by using MinElute Reaction Cleanup Kit (Qiagen).

    Article Title: Synthetic RNA-based logic computation in mammalian cells
    Article Snippet: Reaction mixtures were incubated at 37°C for up to 6 h and then mixed with TURBO DNase (Ambion), and further incubated at 37°C for 30 min to remove the template DNA. .. The resulting mRNAs were purified using a FavorPrep Blood/Cultured Cells total RNA extraction column (Favorgen Biotech, Taiwan), incubated with Antarctic Phosphatase (New England Biolabs) at 37 °C for 30 min, and then purified again using an RNeasy MinElute Cleanup Kit (QIAGEN). .. MiRNA mimics are small, chemically modified double-stranded RNAs that mimic endogenous miRNAs.

    Article Title: CircFAT1 sponges miR-375 to promote the expression of Yes-associated protein 1 in osteosarcoma cells
    Article Snippet: The cell lysates (200 μl) were incubated with 5 μg of control rabbit IgG or antibody against Ago2 (Millipore) coated beads with rotation at 4 °C overnight, respectively. .. After treating with proteinase K buffer, the immunoprecipitated RNAs were extracted by RNeasy MinElute Cleanup Kit (Qiagen) and reversely transcripted using Prime- Script RT Master Mix (TaKaRa).

    Article Title: Activation of defence pathways in Scots pine bark after feeding by pine weevil (Hylobius abietis)
    Article Snippet: The RNA samples from each sample were processed as follows: 1 μg of total RNA was treated with DNase I (Promega, Finland), and incubated for 30 min at 37°C followed by the DNase I inactivation at 65°C for 10 min. .. The treated RNA was then purified using RNeasy MinElute Cleanup Kit according to the manufacturer's instruction (Qiagen, Finland) and eluted in 14 μl nuclease-free water.

    Formalin-fixed Paraffin-Embedded:

    Article Title: MicroRNA Profiling in Mucosal Biopsies of Eosinophilic Esophagitis Patients Pre and Post Treatment with Steroids and Relationship with mRNA Targets
    Article Snippet: RecoverAll Total Nucleic Acid Extraction Kits for FFPE tissues (Ambion, Austin, TX) were used for extraction. .. RNA was further purified and concentrated using the RNEasy Minelute Cleanup Kit (Qiagen, Valencia, CA), and then evaluated by the Agilent Bioanalyzer using an RNA 6000 Nano or Pico LabChip (Agilent Technologies Santa Clara, CA) as described previously .

    Article Title: Amphiregulin (AREG) and Epiregulin (EREG) Gene Expression as Predictor for Overall Survival (OS) in Oxaliplatin/Fluoropyrimidine Plus Bevacizumab Treated mCRC Patients—Analysis of the Phase III AIO KRK-0207 Trial
    Article Snippet: AREG and EREG mRNA expression was measured as published before ( ). .. Tumor tissue was microdissected and RNA was isolated using RNeasy FFPE tissue and RNeasy MinElute CleanUp Kits (both Qiagen, Hilden, Germany) according to the manufacturers' protocol. .. RNA concentration was determined using a NanoDrop ND-1000 device.

    Expressing:

    Article Title: CircFAT1 sponges miR-375 to promote the expression of Yes-associated protein 1 in osteosarcoma cells
    Article Snippet: The Ago-RIP assay was conducted in HOS cells stably expressing vector or shcircFAT1. .. After treating with proteinase K buffer, the immunoprecipitated RNAs were extracted by RNeasy MinElute Cleanup Kit (Qiagen) and reversely transcripted using Prime- Script RT Master Mix (TaKaRa).

    Article Title: Epidermal Growth Factor Receptor Signaling Promotes Pancreatic β-Cell Proliferation in Response to Nutrient Excess in Rats Through mTOR and FOXM1
    Article Snippet: Total RNA was extracted from 150 islets with TRIzol Reagent (Invitrogen, Carlsbad, CA) and purified with RNeasy MinElute cleanup kit (Qiagen, Valencia, CA) following the manufacturer's protocol. .. Microarray analysis was performed on total RNA using the GeneChip Rat Gene 1.0 ST microarrays (five arrays per group; Affymetrix, Santa Clara, CA).

    Article Title: Amphiregulin (AREG) and Epiregulin (EREG) Gene Expression as Predictor for Overall Survival (OS) in Oxaliplatin/Fluoropyrimidine Plus Bevacizumab Treated mCRC Patients—Analysis of the Phase III AIO KRK-0207 Trial
    Article Snippet: Paragraph title: mRNA expression measurement ... Tumor tissue was microdissected and RNA was isolated using RNeasy FFPE tissue and RNeasy MinElute CleanUp Kits (both Qiagen, Hilden, Germany) according to the manufacturers' protocol.

    Modification:

    Article Title: Ancient nuclear genomes enable repatriation of Indigenous human remains
    Article Snippet: The supernatant was combined with 10× volume of a modified binding buffer [500 ml of buffer PB (phosphate buffer; Qiagen), 1:250 pH indicator I, 15 ml of 3 M NaOAC (pH 5.2), and 1.25 ml of NaCl]. .. Extractions were purified using the MinElute Reaction Cleanup Kit (Qiagen) following the manufacturer’s protocol and eluted using 100 μl of buffer EB (elution buffer; Qiagen) after incubation for 10 min at 37°C.

    Article Title: Synthetic RNA-based logic computation in mammalian cells
    Article Snippet: Paragraph title: Preparation of modified mRNA ... The resulting mRNAs were purified using a FavorPrep Blood/Cultured Cells total RNA extraction column (Favorgen Biotech, Taiwan), incubated with Antarctic Phosphatase (New England Biolabs) at 37 °C for 30 min, and then purified again using an RNeasy MinElute Cleanup Kit (QIAGEN).

    RNA Binding Assay:

    Article Title: CircFAT1 sponges miR-375 to promote the expression of Yes-associated protein 1 in osteosarcoma cells
    Article Snippet: RIP experiments were performed by using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, Bedford, MA). .. After treating with proteinase K buffer, the immunoprecipitated RNAs were extracted by RNeasy MinElute Cleanup Kit (Qiagen) and reversely transcripted using Prime- Script RT Master Mix (TaKaRa).

    Hybridization:

    Article Title: Dependence of Intracellular and Exosomal microRNAs on Viral E6/E7 Oncogene Expression in HPV-positive Tumor Cells
    Article Snippet: Due to the low RNA yield, exosome samples were further concentrated to a volume of 6 μl using the RNeasy MinElute Cleanup Kit (Qiagen). .. Small RNA libraries were prepared using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB, Frankfurt/M., Germany) with custom multiplex adaptors and primers (NEBNext Multiplex Oligos for Illumina, Index Primers Set 1).

    Positive Control:

    Article Title: DNA Methylation and Histone Modifications Are the Molecular Lock in Lentivirally Transduced Hematopoietic Progenitor Cells
    Article Snippet: Methylated pL/CMV/GFP (1 μ g) (served as the positive control) and the genomic DNA (1 μ g) from the transduced cells harvested at day 2 and day 7 after transduction were subjected to restriction enzyme digestion prior to bisulfite conversion. .. The restriction digested DNA was purified from the enzymatic reaction by using MinElute Reaction Cleanup Kit (Qiagen).

    Ancient DNA Assay:

    Article Title: Ancient nuclear genomes enable repatriation of Indigenous human remains
    Article Snippet: Paragraph title: Ancient DNA laboratory methods ... Extractions were purified using the MinElute Reaction Cleanup Kit (Qiagen) following the manufacturer’s protocol and eluted using 100 μl of buffer EB (elution buffer; Qiagen) after incubation for 10 min at 37°C.

    Cell Culture:

    Article Title: Different pro-angiogenic potential of γ-irradiated PBMC-derived secretome and its subfractions
    Article Snippet: EVs and the corresponding cultured PBMCs were obtained from 10 different donors, pooled, and lysed by peqGOLD TriFast reagent (VWR peqlab, International GmbH, Darmstadt, Germany). .. RNA cleanup was performed using the RNeasy MinElute Cleanup Kit (Qiagen, Hilden, Germany).

    Generated:

    Article Title: Synthetic RNA-based logic computation in mammalian cells
    Article Snippet: All mRNAs were generated using the above PCR products and MEGAscript T7 Kit (Ambion, USA). .. The resulting mRNAs were purified using a FavorPrep Blood/Cultured Cells total RNA extraction column (Favorgen Biotech, Taiwan), incubated with Antarctic Phosphatase (New England Biolabs) at 37 °C for 30 min, and then purified again using an RNeasy MinElute Cleanup Kit (QIAGEN).

    Article Title: Activation of defence pathways in Scots pine bark after feeding by pine weevil (Hylobius abietis)
    Article Snippet: The treated RNA was then purified using RNeasy MinElute Cleanup Kit according to the manufacturer's instruction (Qiagen, Finland) and eluted in 14 μl nuclease-free water. .. The treated RNA was then purified using RNeasy MinElute Cleanup Kit according to the manufacturer's instruction (Qiagen, Finland) and eluted in 14 μl nuclease-free water.

    other:

    Article Title: Important Role of the GLP-1 Axis for Glucose Homeostasis after Bariatric Surgery
    Article Snippet: Cells were sorted into 2% v/v RNAsin in PBS at 4°C.

    Sequencing:

    Article Title: DNA Methylation and Histone Modifications Are the Molecular Lock in Lentivirally Transduced Hematopoietic Progenitor Cells
    Article Snippet: Paragraph title: 2.5. Sodium Bisulfite Genome Sequencing ... The restriction digested DNA was purified from the enzymatic reaction by using MinElute Reaction Cleanup Kit (Qiagen).

    Article Title: Evolutionary diversity and novelty of DNA repair genes in asexual Bdelloid rotifers
    Article Snippet: Paragraph title: RNA-Seq library construction and sequencing ... Linear acrylamide was from AMRESCO, Solon, OH and the RNeasy MinElute Cleanup Kit was from Qiagen, Germantown, MD.

    Article Title: Dependence of Intracellular and Exosomal microRNAs on Viral E6/E7 Oncogene Expression in HPV-positive Tumor Cells
    Article Snippet: Paragraph title: Small RNA deep sequencing and analysis ... Due to the low RNA yield, exosome samples were further concentrated to a volume of 6 μl using the RNeasy MinElute Cleanup Kit (Qiagen).

    Article Title: Maximum Diastolic Potential of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes Depends Critically on IKr
    Article Snippet: qPCR analysis was performed with the ABI Prism 7000 Sequence Detection System (Applied Biosystems, Foster City, CA, USA). .. Total RNA was extracted with RNAeasy MinElute Cleanup Kit (Qiagen, CA).

    Binding Assay:

    Article Title: Ancient nuclear genomes enable repatriation of Indigenous human remains
    Article Snippet: The supernatant was combined with 10× volume of a modified binding buffer [500 ml of buffer PB (phosphate buffer; Qiagen), 1:250 pH indicator I, 15 ml of 3 M NaOAC (pH 5.2), and 1.25 ml of NaCl]. .. Extractions were purified using the MinElute Reaction Cleanup Kit (Qiagen) following the manufacturer’s protocol and eluted using 100 μl of buffer EB (elution buffer; Qiagen) after incubation for 10 min at 37°C.

    RNA Sequencing Assay:

    Article Title: Evolutionary diversity and novelty of DNA repair genes in asexual Bdelloid rotifers
    Article Snippet: Paragraph title: RNA-Seq library construction and sequencing ... Linear acrylamide was from AMRESCO, Solon, OH and the RNeasy MinElute Cleanup Kit was from Qiagen, Germantown, MD.

    Methylation:

    Article Title: DNA Methylation and Histone Modifications Are the Molecular Lock in Lentivirally Transduced Hematopoietic Progenitor Cells
    Article Snippet: Methylated pL/CMV/GFP (1 μ g) (served as the positive control) and the genomic DNA (1 μ g) from the transduced cells harvested at day 2 and day 7 after transduction were subjected to restriction enzyme digestion prior to bisulfite conversion. .. The restriction digested DNA was purified from the enzymatic reaction by using MinElute Reaction Cleanup Kit (Qiagen).

    Isolation:

    Article Title: Brain region-specific gene expression profiles in freshly isolated rat microglia
    Article Snippet: Paragraph title: RNA Isolation, cDNA Synthesis and Quantitative Real-Time PCR ... Total RNA was further purified using the RNeasy MinElute Cleanup kit (Qiagen).

    Article Title: Extracellular Vesicle-Mediated RNA Release in Histoplasma capsulatum
    Article Snippet: EVs were then isolated from fungal culture supernatants as previously described ( ). .. Small RNA-enriched fractions were isolated using a miRNeasy minikit (Qiagen) and were then treated with an RNeasy MinElute cleanup kit (Qiagen), according to the manufacturer’s protocol, to obtain small RNA-enriched fractions. .. The sRNA profile was assessed in an Agilent 2100 Bioanalyzer (Agilent Technologies).

    Article Title: A Distinct Expression Pattern of the Long 3?-Untranslated Region Dicer mRNA and Its Implications for Posttranscriptional Regulation in Colorectal Cancer
    Article Snippet: Paragraph title: RNA isolation ... Total RNA including miRNA and large RNAs ( > 200 nt) were extracted separately from biopsy materials using the RNeasy Plus Mini Kit and the RNeasy MinElute Cleanup Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions.

    Article Title: Epidermal Growth Factor Receptor Signaling Promotes Pancreatic β-Cell Proliferation in Response to Nutrient Excess in Rats Through mTOR and FOXM1
    Article Snippet: Rat islets were isolated by collagenase digestion and dextran density gradient centrifugation as described previously ( ). .. Total RNA was extracted from 150 islets with TRIzol Reagent (Invitrogen, Carlsbad, CA) and purified with RNeasy MinElute cleanup kit (Qiagen, Valencia, CA) following the manufacturer's protocol.

    Article Title: Different pro-angiogenic potential of γ-irradiated PBMC-derived secretome and its subfractions
    Article Snippet: Paragraph title: RNA isolation and transcriptome analysis ... RNA cleanup was performed using the RNeasy MinElute Cleanup Kit (Qiagen, Hilden, Germany).

    Article Title: Developmental-stage-dependent transcriptional response to leukaemic oncogene expression
    Article Snippet: Before isolation, Trizol was thawed and 200 μl chloroform was added to the Trizol. .. The mixture was loaded onto a RNeasy Minelute column (Qiagen, 74204) and purified as per the manufacturer's instructions.

    Article Title: Amphiregulin (AREG) and Epiregulin (EREG) Gene Expression as Predictor for Overall Survival (OS) in Oxaliplatin/Fluoropyrimidine Plus Bevacizumab Treated mCRC Patients—Analysis of the Phase III AIO KRK-0207 Trial
    Article Snippet: AREG and EREG mRNA expression was measured as published before ( ). .. Tumor tissue was microdissected and RNA was isolated using RNeasy FFPE tissue and RNeasy MinElute CleanUp Kits (both Qiagen, Hilden, Germany) according to the manufacturers' protocol. .. RNA concentration was determined using a NanoDrop ND-1000 device.

    Article Title: Innate immune activation in neonatal tracheal aspirates suggests endotoxin-driven inflammation
    Article Snippet: TA cell pellet RNA was processed immediately or stored at −80°C. .. RNA was isolated by RNA Protect Cell Mini and RNeasy MinElute Cleanup Kits (QIAGEN) per the manufacturer's instructions. .. TA mRNA yield positively correlated with TA cellularity as measured by hemocytometry (data not shown).

    RNA Extraction:

    Article Title: Synthetic RNA-based logic computation in mammalian cells
    Article Snippet: Reaction mixtures were incubated at 37°C for up to 6 h and then mixed with TURBO DNase (Ambion), and further incubated at 37°C for 30 min to remove the template DNA. .. The resulting mRNAs were purified using a FavorPrep Blood/Cultured Cells total RNA extraction column (Favorgen Biotech, Taiwan), incubated with Antarctic Phosphatase (New England Biolabs) at 37 °C for 30 min, and then purified again using an RNeasy MinElute Cleanup Kit (QIAGEN). .. MiRNA mimics are small, chemically modified double-stranded RNAs that mimic endogenous miRNAs.

    Article Title: Activation of defence pathways in Scots pine bark after feeding by pine weevil (Hylobius abietis)
    Article Snippet: The treated RNA was then purified using RNeasy MinElute Cleanup Kit according to the manufacturer's instruction (Qiagen, Finland) and eluted in 14 μl nuclease-free water. .. The treated RNA was then purified using RNeasy MinElute Cleanup Kit according to the manufacturer's instruction (Qiagen, Finland) and eluted in 14 μl nuclease-free water.

    Article Title: MicroRNA Profiling in Mucosal Biopsies of Eosinophilic Esophagitis Patients Pre and Post Treatment with Steroids and Relationship with mRNA Targets
    Article Snippet: Paragraph title: RNA Extraction and Quality Assessment ... RNA was further purified and concentrated using the RNEasy Minelute Cleanup Kit (Qiagen, Valencia, CA), and then evaluated by the Agilent Bioanalyzer using an RNA 6000 Nano or Pico LabChip (Agilent Technologies Santa Clara, CA) as described previously .

    Article Title: Developmental-stage-dependent transcriptional response to leukaemic oncogene expression
    Article Snippet: For RNA extraction, the cell pellet was resuspended in 800 μl Trizol and stored at −80 °C until used. .. The mixture was loaded onto a RNeasy Minelute column (Qiagen, 74204) and purified as per the manufacturer's instructions.

    Labeling:

    Article Title: Epidermal Growth Factor Receptor Signaling Promotes Pancreatic β-Cell Proliferation in Response to Nutrient Excess in Rats Through mTOR and FOXM1
    Article Snippet: Total RNA was extracted from 150 islets with TRIzol Reagent (Invitrogen, Carlsbad, CA) and purified with RNeasy MinElute cleanup kit (Qiagen, Valencia, CA) following the manufacturer's protocol. .. One hundred nanograms of total RNA was processed using the Ambion WT Expression Kit (Invitrogen).

    Purification:

    Article Title: Human non-small cell lung cancer expresses putative cancer stem cell markers and exhibits the transcriptomic profile of multipotent cells
    Article Snippet: Total RNA (1.5 μg) was amplified using the Applause™ WT-Amp ST System (Nugen Technologies, Inc., San Carlos, USA) following the manufacturer’s protocol. .. The seven step amplification process produced ST-cDNA, which was further purified using the MinElute Reaction Cleanup Kit (Qiagen). .. The yield and purity of the purified ST-cDNA were measured using the Nanodrop® ND1000 spectrophotometer.

    Article Title: Ancient nuclear genomes enable repatriation of Indigenous human remains
    Article Snippet: The supernatant was combined with 10× volume of a modified binding buffer [500 ml of buffer PB (phosphate buffer; Qiagen), 1:250 pH indicator I, 15 ml of 3 M NaOAC (pH 5.2), and 1.25 ml of NaCl]. .. Extractions were purified using the MinElute Reaction Cleanup Kit (Qiagen) following the manufacturer’s protocol and eluted using 100 μl of buffer EB (elution buffer; Qiagen) after incubation for 10 min at 37°C. .. Double-stranded Illumina DNA libraries were built according to the methods previously described ( , , ), with some minor modifications in the Taq polymerase used for amplification.

    Article Title: DNA Methylation and Histone Modifications Are the Molecular Lock in Lentivirally Transduced Hematopoietic Progenitor Cells
    Article Snippet: All the samples were digested with 10 U of EcoR1 and AvaI in 1x NE buffer 4 in a total reaction volume of 50 μ L. The reaction mixture was incubated at 37°C for 1 hour. .. The restriction digested DNA was purified from the enzymatic reaction by using MinElute Reaction Cleanup Kit (Qiagen). .. The DNA fragments between 70 bp and 4 kb were purified.

    Article Title: Synthetic RNA-based logic computation in mammalian cells
    Article Snippet: Reaction mixtures were incubated at 37°C for up to 6 h and then mixed with TURBO DNase (Ambion), and further incubated at 37°C for 30 min to remove the template DNA. .. The resulting mRNAs were purified using a FavorPrep Blood/Cultured Cells total RNA extraction column (Favorgen Biotech, Taiwan), incubated with Antarctic Phosphatase (New England Biolabs) at 37 °C for 30 min, and then purified again using an RNeasy MinElute Cleanup Kit (QIAGEN). .. MiRNA mimics are small, chemically modified double-stranded RNAs that mimic endogenous miRNAs.

    Article Title: Brain region-specific gene expression profiles in freshly isolated rat microglia
    Article Snippet: Total RNA (n = 3/brain region) was isolated from sorted microglia per brain region using Trizol Reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. .. Total RNA was further purified using the RNeasy MinElute Cleanup kit (Qiagen). .. RNA was reverse-transcribed into cDNA using the High-Capacity cDNA Reverse Transcription kit (Life Technologies), using 0.5 μg oligo-dT primers and according to the manufacturer’s instructions.

    Article Title: Activation of defence pathways in Scots pine bark after feeding by pine weevil (Hylobius abietis)
    Article Snippet: The RNA samples from each sample were processed as follows: 1 μg of total RNA was treated with DNase I (Promega, Finland), and incubated for 30 min at 37°C followed by the DNase I inactivation at 65°C for 10 min. .. The treated RNA was then purified using RNeasy MinElute Cleanup Kit according to the manufacturer's instruction (Qiagen, Finland) and eluted in 14 μl nuclease-free water. .. RNA quality and integrity were assessed using Agilent RNA 6000 Nano Kit and Agilent 2100 Bioanalyzer following the manufacturer's instruction (Agilent Technologies, Germany).

    Article Title: Epidermal Growth Factor Receptor Signaling Promotes Pancreatic β-Cell Proliferation in Response to Nutrient Excess in Rats Through mTOR and FOXM1
    Article Snippet: Rat islets were isolated by collagenase digestion and dextran density gradient centrifugation as described previously ( ). .. Total RNA was extracted from 150 islets with TRIzol Reagent (Invitrogen, Carlsbad, CA) and purified with RNeasy MinElute cleanup kit (Qiagen, Valencia, CA) following the manufacturer's protocol. .. Microarray analysis was performed on total RNA using the GeneChip Rat Gene 1.0 ST microarrays (five arrays per group; Affymetrix, Santa Clara, CA).

    Article Title: MicroRNA Profiling in Mucosal Biopsies of Eosinophilic Esophagitis Patients Pre and Post Treatment with Steroids and Relationship with mRNA Targets
    Article Snippet: RecoverAll Total Nucleic Acid Extraction Kits for FFPE tissues (Ambion, Austin, TX) were used for extraction. .. RNA was further purified and concentrated using the RNEasy Minelute Cleanup Kit (Qiagen, Valencia, CA), and then evaluated by the Agilent Bioanalyzer using an RNA 6000 Nano or Pico LabChip (Agilent Technologies Santa Clara, CA) as described previously . .. Five nanograms of total RNA was reverse-transcribed using the Taqman MicroRNA Reverse Transcription Kit and the Megaplex RT primer Human Pool A (Applied Biosystems).

    Article Title: Developmental-stage-dependent transcriptional response to leukaemic oncogene expression
    Article Snippet: The aqueous phase was collected and an equal volume of isopropanol was added and mixed. .. The mixture was loaded onto a RNeasy Minelute column (Qiagen, 74204) and purified as per the manufacturer's instructions. .. RNA concentration was determined by a nanodrop and its integrity checked using an Agilent Bioanalyzer.

    Article Title: Innate immune activation in neonatal tracheal aspirates suggests endotoxin-driven inflammation
    Article Snippet: Paragraph title: Purification of TA Pellet RNA ... RNA was isolated by RNA Protect Cell Mini and RNeasy MinElute Cleanup Kits (QIAGEN) per the manufacturer's instructions.

    Polymerase Chain Reaction:

    Article Title: Peripheral T cell cytotoxicity predicts T cell function in the tumor microenvironment
    Article Snippet: A P10EA/P20EA adaptor was ligated to the 5′ end of ds-cDNA and then cut with the NotI restriction enzyme. .. After removal of the adaptor and primer with the MinElute Reaction Cleanup kit (Qiagen), PCR was performed with KAPA HiFi DNA Polymerase (Kapa Biosystems) using TCR α-chain constant region-specific (CA1) or TCR β-chain constant region-specific primers (CB1) and P20EA (Supplementary Table ). .. PCR conditions were as follows: 98 °C (20 sec), 65 °C (30 sec), and 72 °C (1 min) for 20 cycles.

    Article Title: DNA Methylation and Histone Modifications Are the Molecular Lock in Lentivirally Transduced Hematopoietic Progenitor Cells
    Article Snippet: The restriction digested DNA was purified from the enzymatic reaction by using MinElute Reaction Cleanup Kit (Qiagen). .. The restriction digested DNA was purified from the enzymatic reaction by using MinElute Reaction Cleanup Kit (Qiagen).

    Article Title: Synthetic RNA-based logic computation in mammalian cells
    Article Snippet: All mRNAs were generated using the above PCR products and MEGAscript T7 Kit (Ambion, USA). .. The resulting mRNAs were purified using a FavorPrep Blood/Cultured Cells total RNA extraction column (Favorgen Biotech, Taiwan), incubated with Antarctic Phosphatase (New England Biolabs) at 37 °C for 30 min, and then purified again using an RNeasy MinElute Cleanup Kit (QIAGEN).

    Article Title: Activation of defence pathways in Scots pine bark after feeding by pine weevil (Hylobius abietis)
    Article Snippet: The treated RNA was then purified using RNeasy MinElute Cleanup Kit according to the manufacturer's instruction (Qiagen, Finland) and eluted in 14 μl nuclease-free water. .. The treated RNA was then purified using RNeasy MinElute Cleanup Kit according to the manufacturer's instruction (Qiagen, Finland) and eluted in 14 μl nuclease-free water.

    Quantitative RT-PCR:

    Article Title: Epidermal Growth Factor Receptor Signaling Promotes Pancreatic β-Cell Proliferation in Response to Nutrient Excess in Rats Through mTOR and FOXM1
    Article Snippet: Paragraph title: Transcriptomic Profiling and Quantitative RT-PCR Validation ... Total RNA was extracted from 150 islets with TRIzol Reagent (Invitrogen, Carlsbad, CA) and purified with RNeasy MinElute cleanup kit (Qiagen, Valencia, CA) following the manufacturer's protocol.

    Lysis:

    Article Title: CircFAT1 sponges miR-375 to promote the expression of Yes-associated protein 1 in osteosarcoma cells
    Article Snippet: Approximately 1×107 cells were pelleted and re-suspended with an equal pellet volume of RIP Lysis Buffer (about 100 ml) plus protease inhibitors cocktail and RNase inhibitors. .. After treating with proteinase K buffer, the immunoprecipitated RNAs were extracted by RNeasy MinElute Cleanup Kit (Qiagen) and reversely transcripted using Prime- Script RT Master Mix (TaKaRa).

    IA:

    Article Title: Evolutionary diversity and novelty of DNA repair genes in asexual Bdelloid rotifers
    Article Snippet: Linear acrylamide was from AMRESCO, Solon, OH and the RNeasy MinElute Cleanup Kit was from Qiagen, Germantown, MD. .. KAPA Stranded mRNA-Seq Kit and Library Quantification (qPCR) Kit for Illumina platforms were from Kapa Biosystems, Wilmington, MA.

    Plasmid Preparation:

    Article Title: CircFAT1 sponges miR-375 to promote the expression of Yes-associated protein 1 in osteosarcoma cells
    Article Snippet: We first constructed the lentivirus vectors harbouring vector or shcircFAT1, and established two stable cell lines of HOS. .. After treating with proteinase K buffer, the immunoprecipitated RNAs were extracted by RNeasy MinElute Cleanup Kit (Qiagen) and reversely transcripted using Prime- Script RT Master Mix (TaKaRa).

    Software:

    Article Title: Different pro-angiogenic potential of γ-irradiated PBMC-derived secretome and its subfractions
    Article Snippet: RNA cleanup was performed using the RNeasy MinElute Cleanup Kit (Qiagen, Hilden, Germany). .. RNA cleanup was performed using the RNeasy MinElute Cleanup Kit (Qiagen, Hilden, Germany).

    SYBR Green Assay:

    Article Title: Brain region-specific gene expression profiles in freshly isolated rat microglia
    Article Snippet: Total RNA was further purified using the RNeasy MinElute Cleanup kit (Qiagen). .. Total RNA was further purified using the RNeasy MinElute Cleanup kit (Qiagen).

    Article Title: Maximum Diastolic Potential of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes Depends Critically on IKr
    Article Snippet: Total RNA was extracted with RNAeasy MinElute Cleanup Kit (Qiagen, CA). .. 100 ng total RNA from each of the pooled clusters ranging from 10–119 days beating clusters were reverse transcribed with SuperScript™ First Strand Synthesis System for RT-PCR (Invitrogen, CA).

    Multiplex Assay:

    Article Title: Dependence of Intracellular and Exosomal microRNAs on Viral E6/E7 Oncogene Expression in HPV-positive Tumor Cells
    Article Snippet: Due to the low RNA yield, exosome samples were further concentrated to a volume of 6 μl using the RNeasy MinElute Cleanup Kit (Qiagen). .. Due to the low RNA yield, exosome samples were further concentrated to a volume of 6 μl using the RNeasy MinElute Cleanup Kit (Qiagen).

    Agarose Gel Electrophoresis:

    Article Title: Evolutionary diversity and novelty of DNA repair genes in asexual Bdelloid rotifers
    Article Snippet: Linear acrylamide was from AMRESCO, Solon, OH and the RNeasy MinElute Cleanup Kit was from Qiagen, Germantown, MD. .. Illumina TruSeq indexed adapters were from Integrated DNA Technologies (IDT), Coralville, IA.

    Next-Generation Sequencing:

    Article Title: Peripheral T cell cytotoxicity predicts T cell function in the tumor microenvironment
    Article Snippet: A next-generation sequencing analysis was performed with unbiased TCR repertoire analysis technology developed by Repertoire Genesis Inc.. Unbiased adaptor-ligation PCR was performed according to a previous study . .. After removal of the adaptor and primer with the MinElute Reaction Cleanup kit (Qiagen), PCR was performed with KAPA HiFi DNA Polymerase (Kapa Biosystems) using TCR α-chain constant region-specific (CA1) or TCR β-chain constant region-specific primers (CB1) and P20EA (Supplementary Table ).

    Laser Capture Microdissection:

    Article Title: MicroRNA Profiling in Mucosal Biopsies of Eosinophilic Esophagitis Patients Pre and Post Treatment with Steroids and Relationship with mRNA Targets
    Article Snippet: About 20,000 epithelial cells were captured on LCM Macro CapSure caps (Applied Biosystems) using the Arcturus XT LCM instrument (Applied Biosystems) and the captured cells in the caps were used to extract total RNA. .. RNA was further purified and concentrated using the RNEasy Minelute Cleanup Kit (Qiagen, Valencia, CA), and then evaluated by the Agilent Bioanalyzer using an RNA 6000 Nano or Pico LabChip (Agilent Technologies Santa Clara, CA) as described previously .

    Random Hexamer Labeling:

    Article Title: Amphiregulin (AREG) and Epiregulin (EREG) Gene Expression as Predictor for Overall Survival (OS) in Oxaliplatin/Fluoropyrimidine Plus Bevacizumab Treated mCRC Patients—Analysis of the Phase III AIO KRK-0207 Trial
    Article Snippet: Tumor tissue was microdissected and RNA was isolated using RNeasy FFPE tissue and RNeasy MinElute CleanUp Kits (both Qiagen, Hilden, Germany) according to the manufacturers' protocol. .. Tumor tissue was microdissected and RNA was isolated using RNeasy FFPE tissue and RNeasy MinElute CleanUp Kits (both Qiagen, Hilden, Germany) according to the manufacturers' protocol.

    Spectrophotometry:

    Article Title: Innate immune activation in neonatal tracheal aspirates suggests endotoxin-driven inflammation
    Article Snippet: RNA was isolated by RNA Protect Cell Mini and RNeasy MinElute Cleanup Kits (QIAGEN) per the manufacturer's instructions. .. RNA was isolated by RNA Protect Cell Mini and RNeasy MinElute Cleanup Kits (QIAGEN) per the manufacturer's instructions.

    Produced:

    Article Title: Human non-small cell lung cancer expresses putative cancer stem cell markers and exhibits the transcriptomic profile of multipotent cells
    Article Snippet: Total RNA (1.5 μg) was amplified using the Applause™ WT-Amp ST System (Nugen Technologies, Inc., San Carlos, USA) following the manufacturer’s protocol. .. The seven step amplification process produced ST-cDNA, which was further purified using the MinElute Reaction Cleanup Kit (Qiagen). .. The yield and purity of the purified ST-cDNA were measured using the Nanodrop® ND1000 spectrophotometer.

    Immunoprecipitation:

    Article Title: CircFAT1 sponges miR-375 to promote the expression of Yes-associated protein 1 in osteosarcoma cells
    Article Snippet: The cell lysates (200 μl) were incubated with 5 μg of control rabbit IgG or antibody against Ago2 (Millipore) coated beads with rotation at 4 °C overnight, respectively. .. After treating with proteinase K buffer, the immunoprecipitated RNAs were extracted by RNeasy MinElute Cleanup Kit (Qiagen) and reversely transcripted using Prime- Script RT Master Mix (TaKaRa). .. The abundance of circFAT1 level was detected by qRT–PCR assay.

    Staining:

    Article Title: MicroRNA Profiling in Mucosal Biopsies of Eosinophilic Esophagitis Patients Pre and Post Treatment with Steroids and Relationship with mRNA Targets
    Article Snippet: If biopsy sections contained sub-epithelium, the sections were deparaffinized, stained, dehydrated through graded alcohols using the Paradise FFPE reagent System (Applied Biosystems, Foster City, CA) and subjected to LCM within 2 hours of deparaffinization. .. RNA was further purified and concentrated using the RNEasy Minelute Cleanup Kit (Qiagen, Valencia, CA), and then evaluated by the Agilent Bioanalyzer using an RNA 6000 Nano or Pico LabChip (Agilent Technologies Santa Clara, CA) as described previously .

    Gradient Centrifugation:

    Article Title: Epidermal Growth Factor Receptor Signaling Promotes Pancreatic β-Cell Proliferation in Response to Nutrient Excess in Rats Through mTOR and FOXM1
    Article Snippet: Rat islets were isolated by collagenase digestion and dextran density gradient centrifugation as described previously ( ). .. Total RNA was extracted from 150 islets with TRIzol Reagent (Invitrogen, Carlsbad, CA) and purified with RNeasy MinElute cleanup kit (Qiagen, Valencia, CA) following the manufacturer's protocol.

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    Qiagen purification kit rneasy minelute
    Fibroblasts from bleomycin-injured mice are associated with decreased histone acetylation. ChIP using antibodies against ( a ) acetylated H3 ( n =6 for saline, n =5 for bleomycin) and ( b ) acetylated H4 ( n =6 for saline, n =4 for bleomycin) was performed in fibroblasts from saline- and bleomycin-treated mice. Relative enrichment of the Fas promoter, normalized to saline control, was assayed by real-time PCR from <t>immunoprecipitated</t> <t>DNA.</t> ( c ) Expression of HDAC2 and HDAC4 were assayed by immunoblot in fibroblasts from bleomycin- and saline-treated mice, with mean densitometry values shown ( n =4). * P
    Purification Kit Rneasy Minelute, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Fibroblasts from bleomycin-injured mice are associated with decreased histone acetylation. ChIP using antibodies against ( a ) acetylated H3 ( n =6 for saline, n =5 for bleomycin) and ( b ) acetylated H4 ( n =6 for saline, n =4 for bleomycin) was performed in fibroblasts from saline- and bleomycin-treated mice. Relative enrichment of the Fas promoter, normalized to saline control, was assayed by real-time PCR from immunoprecipitated DNA. ( c ) Expression of HDAC2 and HDAC4 were assayed by immunoblot in fibroblasts from bleomycin- and saline-treated mice, with mean densitometry values shown ( n =4). * P

    Journal: Cell Death & Disease

    Article Title: Histone modifications are responsible for decreased Fas expression and apoptosis resistance in fibrotic lung fibroblasts

    doi: 10.1038/cddis.2013.146

    Figure Lengend Snippet: Fibroblasts from bleomycin-injured mice are associated with decreased histone acetylation. ChIP using antibodies against ( a ) acetylated H3 ( n =6 for saline, n =5 for bleomycin) and ( b ) acetylated H4 ( n =6 for saline, n =4 for bleomycin) was performed in fibroblasts from saline- and bleomycin-treated mice. Relative enrichment of the Fas promoter, normalized to saline control, was assayed by real-time PCR from immunoprecipitated DNA. ( c ) Expression of HDAC2 and HDAC4 were assayed by immunoblot in fibroblasts from bleomycin- and saline-treated mice, with mean densitometry values shown ( n =4). * P

    Article Snippet: Immunoprecipitated chromatin was reverse cross-linked and DNA was cleaned using the DNA cleanup kit (Qiagen).

    Techniques: Mouse Assay, Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction, Immunoprecipitation, Expressing

    The Fas gene promoter in IPF fibroblasts was also associated with decreased histone acetylation and increased H3K9 trimethylation. ChIP using antibodies against ( b ) acetylated H3 and ( c ) H3K9Me3 was performed in fibroblasts from IPF patients and nonfibrotic controls. ( a ) Immunoprecipitated DNA was PCR amplified using primer pairs from two separate regions of the Fas gene promoter. * P

    Journal: Cell Death & Disease

    Article Title: Histone modifications are responsible for decreased Fas expression and apoptosis resistance in fibrotic lung fibroblasts

    doi: 10.1038/cddis.2013.146

    Figure Lengend Snippet: The Fas gene promoter in IPF fibroblasts was also associated with decreased histone acetylation and increased H3K9 trimethylation. ChIP using antibodies against ( b ) acetylated H3 and ( c ) H3K9Me3 was performed in fibroblasts from IPF patients and nonfibrotic controls. ( a ) Immunoprecipitated DNA was PCR amplified using primer pairs from two separate regions of the Fas gene promoter. * P

    Article Snippet: Immunoprecipitated chromatin was reverse cross-linked and DNA was cleaned using the DNA cleanup kit (Qiagen).

    Techniques: Chromatin Immunoprecipitation, Immunoprecipitation, Polymerase Chain Reaction, Amplification

    Lowest p -values of clusters established by the pipeline. The p -values are arranged by feature of the strongest significant association of each of the 6165 clusters. The 50 features involved as strongest associations have been coloured by type: biological (red), methodological (green), and technical (blue). The boxes span the first and third quartiles. The dark band inside each box represents the median. The whiskers of the boxes extend to the lowest and highest values within a distance of 1.5 times the interquartile range. As can be seen, most p -values were above 1e-24, but a few methodological features have associated clusters with very low p -values, such as f056, f068, f069, f076, f079, and f084. The library preparation kit ScriptSeq v2 RNA-Seq, Illumina (f084) displays strongly associated clusters with p -values as low as 3.04e-89 that mapped as species Avian myeloblastosis-associated virus . Clusters that were annotated as NCBI species Parvovirus NIH/CQV were associated to laboratory-kit RNeasy MinElute, Qiagen (f076) with minimal p -value 5.48e-38. Finally, a cluster annotated as Acanthocystis turfacea chlorella virus MN0810.1 (ATCV) was associated to DNase/RNase: Promega DNase stop solution (f069) with p -value = 4.19e-12.

    Journal: Viruses

    Article Title: Identification of Known and Novel Recurrent Viral Sequences in Data from Multiple Patients and Multiple Cancers

    doi: 10.3390/v8020053

    Figure Lengend Snippet: Lowest p -values of clusters established by the pipeline. The p -values are arranged by feature of the strongest significant association of each of the 6165 clusters. The 50 features involved as strongest associations have been coloured by type: biological (red), methodological (green), and technical (blue). The boxes span the first and third quartiles. The dark band inside each box represents the median. The whiskers of the boxes extend to the lowest and highest values within a distance of 1.5 times the interquartile range. As can be seen, most p -values were above 1e-24, but a few methodological features have associated clusters with very low p -values, such as f056, f068, f069, f076, f079, and f084. The library preparation kit ScriptSeq v2 RNA-Seq, Illumina (f084) displays strongly associated clusters with p -values as low as 3.04e-89 that mapped as species Avian myeloblastosis-associated virus . Clusters that were annotated as NCBI species Parvovirus NIH/CQV were associated to laboratory-kit RNeasy MinElute, Qiagen (f076) with minimal p -value 5.48e-38. Finally, a cluster annotated as Acanthocystis turfacea chlorella virus MN0810.1 (ATCV) was associated to DNase/RNase: Promega DNase stop solution (f069) with p -value = 4.19e-12.

    Article Snippet: Most p -values were above 1e-24 and associations with lower p -values were to a few methodological features annotated as extraction kits: QIAamp DNA mini kit (f056) (Qiagen, Hilden, Germany), DNase/RNase: Promega DNase (f068) (Promega, Madison, WI, USA), and DNase/RNase: Promega DNase stop solution (f069), purification kit: RNeasy MinElute, Qiagen (f076), library build: NEBNext, New England BioLabs (f079) (New England Biolabs, Ipswitch, MA, USA), and ScriptSeq v2 RNA-Seq, Illumina (f084); the latter with a minimum p -value of 3.04e-89.

    Techniques: RNA Sequencing Assay