nhbe  (Qiagen)

 
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    Name:
    RNeasy 96 Kit
    Description:
    For 96 well purification of total RNA from cells Kit contents Qiagen RNeasy 96 Kit 4 preps 45 to 140L Elution Volume 5 x 10e5 Sample Cultured Cells Sample Total and Cytoplasmic RNA Purification Silica Technology Manual Processing 96 well Plate Format 30 to 40 min Time Run 1 3 to 3 1g Yield Ideal for PCR qPCR Real time PCR Includes 4 RNeasy 96 Plates Elution Microtubes CL Caps S Blocks AirPore Tape Sheets RNase free Reagents and Buffers Benefits High throughput RNA purification Fast and convenient sample processing Reproducible yields from 10 to 500 000 cells High quality RNA for any application No organic extraction or precipitation
    Catalog Number:
    74181
    Price:
    1590
    Category:
    RNeasy 96 Kit
    Buy from Supplier


    Structured Review

    Qiagen nhbe
    RNeasy 96 Kit
    For 96 well purification of total RNA from cells Kit contents Qiagen RNeasy 96 Kit 4 preps 45 to 140L Elution Volume 5 x 10e5 Sample Cultured Cells Sample Total and Cytoplasmic RNA Purification Silica Technology Manual Processing 96 well Plate Format 30 to 40 min Time Run 1 3 to 3 1g Yield Ideal for PCR qPCR Real time PCR Includes 4 RNeasy 96 Plates Elution Microtubes CL Caps S Blocks AirPore Tape Sheets RNase free Reagents and Buffers Benefits High throughput RNA purification Fast and convenient sample processing Reproducible yields from 10 to 500 000 cells High quality RNA for any application No organic extraction or precipitation
    https://www.bioz.com/result/nhbe/product/Qiagen
    Average 90 stars, based on 19160 article reviews
    Price from $9.99 to $1999.99
    nhbe - by Bioz Stars, 2020-05
    90/100 stars

    Images

    1) Product Images from "The species translation challenge—A systems biology perspective on human and rat bronchial epithelial cells"

    Article Title: The species translation challenge—A systems biology perspective on human and rat bronchial epithelial cells

    Journal: Scientific Data

    doi: 10.1038/sdata.2014.9

    The determination of optimal time points for phosphoproteomics measurements in NHBE and NRBE. Human and rat bronchial epithelial cells were treated with seven stimuli at five different time points (0, 5, 15, 20 and 25 min). The time course of the raw data (fluorescent intensity: FI) for each phosphoprotein was plotted in subplots using a modified version of DataRail. The solid fill colours (yellow, green, purple, grey/black) of the time course correspond to different signal behaviour over time according to the DataRail colouring scheme. Yellow colour corresponds to transient activity (FI increases and then decreases), green colour corresponds to sustained activity (FI increases and remains active), purple colour corresponds to late activity (FI starts stable and then increases) and grey/black to no change (FI increase/decrease compare to basal level at 0 time point less than 50% across all time points - the darker the grey colour the bigger the average FI). In the majority of experiments, maximum phosphoprotein activation in NHBE cells was found at 5 (red) and 25 (blue) minutes, whereas NRBE cells were maximally activated at 20 (green) and 25 (blue) minutes. Thus, 5 and 25 min were selected as the optimal time points for both cell types.
    Figure Legend Snippet: The determination of optimal time points for phosphoproteomics measurements in NHBE and NRBE. Human and rat bronchial epithelial cells were treated with seven stimuli at five different time points (0, 5, 15, 20 and 25 min). The time course of the raw data (fluorescent intensity: FI) for each phosphoprotein was plotted in subplots using a modified version of DataRail. The solid fill colours (yellow, green, purple, grey/black) of the time course correspond to different signal behaviour over time according to the DataRail colouring scheme. Yellow colour corresponds to transient activity (FI increases and then decreases), green colour corresponds to sustained activity (FI increases and remains active), purple colour corresponds to late activity (FI starts stable and then increases) and grey/black to no change (FI increase/decrease compare to basal level at 0 time point less than 50% across all time points - the darker the grey colour the bigger the average FI). In the majority of experiments, maximum phosphoprotein activation in NHBE cells was found at 5 (red) and 25 (blue) minutes, whereas NRBE cells were maximally activated at 20 (green) and 25 (blue) minutes. Thus, 5 and 25 min were selected as the optimal time points for both cell types.

    Techniques Used: Modification, Activity Assay, Activation Assay

    2) Product Images from "Preclinical Profile of VX-950, a Potent, Selective, and Orally Bioavailable Inhibitor of Hepatitis C Virus NS3-4A Serine Protease"

    Article Title: Preclinical Profile of VX-950, a Potent, Selective, and Orally Bioavailable Inhibitor of Hepatitis C Virus NS3-4A Serine Protease

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.50.3.899-909.2006

    Concentration-dependent reduction of HCV replicon RNA levels by VX-950. HCV replicon cells were incubated with various concentrations of VX-950 for 48 h. At the end of the 48-h incubation, total cellular RNA was extracted by RNeasy-96. The level of HCV replicon RNA remaining was then determined by QRT-PCR, as described in Materials and Methods, and was shown as a percentage of replicon RNA levels in control cells incubated with 0.5% DMSO. Each bar represents the average of results from 5 cell culture replicates with the SD. Data for one representative IC 50 determination is shown.
    Figure Legend Snippet: Concentration-dependent reduction of HCV replicon RNA levels by VX-950. HCV replicon cells were incubated with various concentrations of VX-950 for 48 h. At the end of the 48-h incubation, total cellular RNA was extracted by RNeasy-96. The level of HCV replicon RNA remaining was then determined by QRT-PCR, as described in Materials and Methods, and was shown as a percentage of replicon RNA levels in control cells incubated with 0.5% DMSO. Each bar represents the average of results from 5 cell culture replicates with the SD. Data for one representative IC 50 determination is shown.

    Techniques Used: Concentration Assay, Incubation, Quantitative RT-PCR, Cell Culture

    3) Product Images from "The species translation challenge—A systems biology perspective on human and rat bronchial epithelial cells"

    Article Title: The species translation challenge—A systems biology perspective on human and rat bronchial epithelial cells

    Journal: Scientific Data

    doi: 10.1038/sdata.2014.9

    The determination of optimal time points for phosphoproteomics measurements in NHBE and NRBE. Human and rat bronchial epithelial cells were treated with seven stimuli at five different time points (0, 5, 15, 20 and 25 min). The time course of the raw data (fluorescent intensity: FI) for each phosphoprotein was plotted in subplots using a modified version of DataRail. The solid fill colours (yellow, green, purple, grey/black) of the time course correspond to different signal behaviour over time according to the DataRail colouring scheme. Yellow colour corresponds to transient activity (FI increases and then decreases), green colour corresponds to sustained activity (FI increases and remains active), purple colour corresponds to late activity (FI starts stable and then increases) and grey/black to no change (FI increase/decrease compare to basal level at 0 time point less than 50% across all time points - the darker the grey colour the bigger the average FI). In the majority of experiments, maximum phosphoprotein activation in NHBE cells was found at 5 (red) and 25 (blue) minutes, whereas NRBE cells were maximally activated at 20 (green) and 25 (blue) minutes. Thus, 5 and 25 min were selected as the optimal time points for both cell types.
    Figure Legend Snippet: The determination of optimal time points for phosphoproteomics measurements in NHBE and NRBE. Human and rat bronchial epithelial cells were treated with seven stimuli at five different time points (0, 5, 15, 20 and 25 min). The time course of the raw data (fluorescent intensity: FI) for each phosphoprotein was plotted in subplots using a modified version of DataRail. The solid fill colours (yellow, green, purple, grey/black) of the time course correspond to different signal behaviour over time according to the DataRail colouring scheme. Yellow colour corresponds to transient activity (FI increases and then decreases), green colour corresponds to sustained activity (FI increases and remains active), purple colour corresponds to late activity (FI starts stable and then increases) and grey/black to no change (FI increase/decrease compare to basal level at 0 time point less than 50% across all time points - the darker the grey colour the bigger the average FI). In the majority of experiments, maximum phosphoprotein activation in NHBE cells was found at 5 (red) and 25 (blue) minutes, whereas NRBE cells were maximally activated at 20 (green) and 25 (blue) minutes. Thus, 5 and 25 min were selected as the optimal time points for both cell types.

    Techniques Used: Modification, Activity Assay, Activation Assay

    4) Product Images from "Preclinical Profile of VX-950, a Potent, Selective, and Orally Bioavailable Inhibitor of Hepatitis C Virus NS3-4A Serine Protease"

    Article Title: Preclinical Profile of VX-950, a Potent, Selective, and Orally Bioavailable Inhibitor of Hepatitis C Virus NS3-4A Serine Protease

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.50.3.899-909.2006

    Concentration-dependent reduction of HCV replicon RNA levels by VX-950. HCV replicon cells were incubated with various concentrations of VX-950 for 48 h. At the end of the 48-h incubation, total cellular RNA was extracted by RNeasy-96. The level of HCV replicon RNA remaining was then determined by QRT-PCR, as described in Materials and Methods, and was shown as a percentage of replicon RNA levels in control cells incubated with 0.5% DMSO. Each bar represents the average of results from 5 cell culture replicates with the SD. Data for one representative IC 50 determination is shown.
    Figure Legend Snippet: Concentration-dependent reduction of HCV replicon RNA levels by VX-950. HCV replicon cells were incubated with various concentrations of VX-950 for 48 h. At the end of the 48-h incubation, total cellular RNA was extracted by RNeasy-96. The level of HCV replicon RNA remaining was then determined by QRT-PCR, as described in Materials and Methods, and was shown as a percentage of replicon RNA levels in control cells incubated with 0.5% DMSO. Each bar represents the average of results from 5 cell culture replicates with the SD. Data for one representative IC 50 determination is shown.

    Techniques Used: Concentration Assay, Incubation, Quantitative RT-PCR, Cell Culture

    5) Product Images from "Combination of a Hepatitis C Virus NS3-NS4A Protease Inhibitor and Alpha Interferon Synergistically Inhibits Viral RNA Replication and Facilitates Viral RNA Clearance in Replicon Cells"

    Article Title: Combination of a Hepatitis C Virus NS3-NS4A Protease Inhibitor and Alpha Interferon Synergistically Inhibits Viral RNA Replication and Facilitates Viral RNA Clearance in Replicon Cells

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.48.12.4784-4792.2004

    Dose-dependent inhibition of HCV replicon by PI-1 or IFN-α alone. HCV replicon cells were treated with PI-1 (A) or IFN-α (B) for 48 h. At the end of the 48-h treatment, total cellular RNA was extracted with the RNeasy-96 kit. The levels
    Figure Legend Snippet: Dose-dependent inhibition of HCV replicon by PI-1 or IFN-α alone. HCV replicon cells were treated with PI-1 (A) or IFN-α (B) for 48 h. At the end of the 48-h treatment, total cellular RNA was extracted with the RNeasy-96 kit. The levels

    Techniques Used: Inhibition

    Related Articles

    Multiplex Assay:

    Article Title: Preclinical Profile of VX-950, a Potent, Selective, and Orally Bioavailable Inhibitor of Hepatitis C Virus NS3-4A Serine Protease
    Article Snippet: .. Total cellular RNA was extracted using an RNeasy-96 kit (QIAGEN, Valencia, CA), and the copy number of the HCV RNA was determined in a quantitative, real-time, multiplex reverse transcription-PCR (QRT-PCR, or Taqman) assay ( ). .. The cytotoxicity of compounds in the HCV replicon cells was measured under the same experimental settings using the tetrazolium-based cell viability assay as described before ( , ).

    TaqMan Assay:

    Article Title: Preclinical Profile of VX-950, a Potent, Selective, and Orally Bioavailable Inhibitor of Hepatitis C Virus NS3-4A Serine Protease
    Article Snippet: .. Total cellular RNA was extracted using an RNeasy-96 kit (QIAGEN, Valencia, CA), and the copy number of the HCV RNA was determined in a quantitative, real-time, multiplex reverse transcription-PCR (QRT-PCR, or Taqman) assay ( ). .. The cytotoxicity of compounds in the HCV replicon cells was measured under the same experimental settings using the tetrazolium-based cell viability assay as described before ( , ).

    Incubation:

    Article Title: Combination of a Hepatitis C Virus NS3-NS4A Protease Inhibitor and Alpha Interferon Synergistically Inhibits Viral RNA Replication and Facilitates Viral RNA Clearance in Replicon Cells
    Article Snippet: .. After the cells were incubated with the compounds for 48 h, the intracellular RNA was extracted with an RNeasy 96 kit (Qiagen, Valencia, Calif.). .. The level of HCV RNA, both the positive strand and the negative strand, was determined by a real-time multiplex quantitative reverse transcription-PCR (RT-PCR) assay (the Taqman assay) with a pair of HCV-specific primers (5′-CCA TGA ATC ACT CCC CTG TG-3′ and 5′-CCG GTC GTC CTG GCA ATT C-3′), an HCV-specific probe (5′-6-FAM-CCT GGA GGC TGC ACG ACA CTC A-TAMRA-3′, where FAM is 6-carboxyfluorescein and TAMRA is 6-carboxytetramethylrhodamine), and an ABI Prism 7700 sequence detection system (Applied Biosystems, Foster City, Calif.).

    Article Title: Preclinical Profile of VX-950, a Potent, Selective, and Orally Bioavailable Inhibitor of Hepatitis C Virus NS3-4A Serine Protease
    Article Snippet: .. Various concentrations of VX-950 diluted in IM-HDM were added to the cell culture and incubated for 48 h. The medium was removed and replaced in its entirety with IM-HDM containing the same concentrations of VX-950 and incubated for an additional 72 h. The cell monolayer was rinsed three times and lysed, and the total cellular RNA was extracted with the RNeasy-96 kit according to the manufacturer's instructions (QIAGEN). .. HCV RNA levels were determined using the QRT-PCR method as described above.

    Cell Culture:

    Article Title: Preclinical Profile of VX-950, a Potent, Selective, and Orally Bioavailable Inhibitor of Hepatitis C Virus NS3-4A Serine Protease
    Article Snippet: .. Various concentrations of VX-950 diluted in IM-HDM were added to the cell culture and incubated for 48 h. The medium was removed and replaced in its entirety with IM-HDM containing the same concentrations of VX-950 and incubated for an additional 72 h. The cell monolayer was rinsed three times and lysed, and the total cellular RNA was extracted with the RNeasy-96 kit according to the manufacturer's instructions (QIAGEN). .. HCV RNA levels were determined using the QRT-PCR method as described above.

    Quantitative RT-PCR:

    Article Title: Preclinical Profile of VX-950, a Potent, Selective, and Orally Bioavailable Inhibitor of Hepatitis C Virus NS3-4A Serine Protease
    Article Snippet: .. Total cellular RNA was extracted using an RNeasy-96 kit (QIAGEN, Valencia, CA), and the copy number of the HCV RNA was determined in a quantitative, real-time, multiplex reverse transcription-PCR (QRT-PCR, or Taqman) assay ( ). .. The cytotoxicity of compounds in the HCV replicon cells was measured under the same experimental settings using the tetrazolium-based cell viability assay as described before ( , ).

    Article Title: Synthetic oligonucleotides recruit ILF2/3 to RNA transcripts to modulate splicing
    Article Snippet: .. Ten microliters of lysate were used to isolate RNA with the RNeasy 96 Kit (Qiagen), and real-time RT-PCR was performed as described above. .. The SMN2 expression level was normalized to that of the mouse gene Gapdh, and this was further normalized to the level in PBS-treated mice.

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  • 99
    Qiagen rneasy 96 kit
    Concentration-dependent reduction of HCV replicon RNA levels by VX-950. HCV replicon cells were incubated with various concentrations of VX-950 for 48 h. At the end of the 48-h incubation, total cellular RNA was extracted by <t>RNeasy-96.</t> The level of HCV replicon RNA remaining was then determined by QRT-PCR, as described in Materials and Methods, and was shown as a percentage of replicon RNA levels in control cells incubated with 0.5% DMSO. Each bar represents the average of results from 5 cell culture replicates with the SD. Data for one representative IC 50 determination is shown.
    Rneasy 96 Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy 96 kit/product/Qiagen
    Average 99 stars, based on 45 article reviews
    Price from $9.99 to $1999.99
    rneasy 96 kit - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

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    Concentration-dependent reduction of HCV replicon RNA levels by VX-950. HCV replicon cells were incubated with various concentrations of VX-950 for 48 h. At the end of the 48-h incubation, total cellular RNA was extracted by RNeasy-96. The level of HCV replicon RNA remaining was then determined by QRT-PCR, as described in Materials and Methods, and was shown as a percentage of replicon RNA levels in control cells incubated with 0.5% DMSO. Each bar represents the average of results from 5 cell culture replicates with the SD. Data for one representative IC 50 determination is shown.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Preclinical Profile of VX-950, a Potent, Selective, and Orally Bioavailable Inhibitor of Hepatitis C Virus NS3-4A Serine Protease

    doi: 10.1128/AAC.50.3.899-909.2006

    Figure Lengend Snippet: Concentration-dependent reduction of HCV replicon RNA levels by VX-950. HCV replicon cells were incubated with various concentrations of VX-950 for 48 h. At the end of the 48-h incubation, total cellular RNA was extracted by RNeasy-96. The level of HCV replicon RNA remaining was then determined by QRT-PCR, as described in Materials and Methods, and was shown as a percentage of replicon RNA levels in control cells incubated with 0.5% DMSO. Each bar represents the average of results from 5 cell culture replicates with the SD. Data for one representative IC 50 determination is shown.

    Article Snippet: Total cellular RNA was extracted using an RNeasy-96 kit (QIAGEN, Valencia, CA), and the copy number of the HCV RNA was determined in a quantitative, real-time, multiplex reverse transcription-PCR (QRT-PCR, or Taqman) assay ( ).

    Techniques: Concentration Assay, Incubation, Quantitative RT-PCR, Cell Culture

    Dose-dependent inhibition of HCV replicon by PI-1 or IFN-α alone. HCV replicon cells were treated with PI-1 (A) or IFN-α (B) for 48 h. At the end of the 48-h treatment, total cellular RNA was extracted with the RNeasy-96 kit. The levels

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Combination of a Hepatitis C Virus NS3-NS4A Protease Inhibitor and Alpha Interferon Synergistically Inhibits Viral RNA Replication and Facilitates Viral RNA Clearance in Replicon Cells

    doi: 10.1128/AAC.48.12.4784-4792.2004

    Figure Lengend Snippet: Dose-dependent inhibition of HCV replicon by PI-1 or IFN-α alone. HCV replicon cells were treated with PI-1 (A) or IFN-α (B) for 48 h. At the end of the 48-h treatment, total cellular RNA was extracted with the RNeasy-96 kit. The levels

    Article Snippet: After the cells were incubated with the compounds for 48 h, the intracellular RNA was extracted with an RNeasy 96 kit (Qiagen, Valencia, Calif.).

    Techniques: Inhibition