rneasy mini kit 74104  (Qiagen)


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    Name:
    RNeasy Mini Kit
    Description:
    For purification of up to 100 µg total RNA from cells tissues and yeast Kit contents Qiagen RNeasy Mini Kit 50 preps 0 5 to 30mg Sample 30 to 100L Elution Volume Tissue Cells Sample Total RNA Purification Silica Technology Spin Column Format Ideal for Northern Dot and Slot Blotting End point RT PCR Quantitative Real time RT PCR For Purification of Up to 100g Total RNA from Cells Tissues and Yeast Includes 250 RNeasy Mini Spin Columns Collection Tubes 1 5mL and 2mL RNase free Reagents and Buffers Benefits Fast procedure delivering high quality total RNA in minutes Ready to use RNA for high performance in any downstream application Consistent RNA yields from small amounts of starting material No phenol chloroform extraction no CsCl gradients no LiCl or ethanol precipitatio
    Catalog Number:
    74104
    Price:
    314
    Category:
    RNeasy Mini Kit
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    Structured Review

    Qiagen rneasy mini kit 74104
    RNeasy Mini Kit
    For purification of up to 100 µg total RNA from cells tissues and yeast Kit contents Qiagen RNeasy Mini Kit 50 preps 0 5 to 30mg Sample 30 to 100L Elution Volume Tissue Cells Sample Total RNA Purification Silica Technology Spin Column Format Ideal for Northern Dot and Slot Blotting End point RT PCR Quantitative Real time RT PCR For Purification of Up to 100g Total RNA from Cells Tissues and Yeast Includes 250 RNeasy Mini Spin Columns Collection Tubes 1 5mL and 2mL RNase free Reagents and Buffers Benefits Fast procedure delivering high quality total RNA in minutes Ready to use RNA for high performance in any downstream application Consistent RNA yields from small amounts of starting material No phenol chloroform extraction no CsCl gradients no LiCl or ethanol precipitatio
    https://www.bioz.com/result/rneasy mini kit 74104/product/Qiagen
    Average 90 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    rneasy mini kit 74104 - by Bioz Stars, 2020-01
    90/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Respiratory syncytial virus exhibits differential tropism for distinct human placental cell types with Hofbauer cells acting as a permissive reservoir for infection
    Article Snippet: .. Quantitative polymerase chain reaction (qPCR) Cells were scraped and homogenized in RLT buffer with 1% betamercaptoethanol by vortexing and centrifugation through QiaShredder tubes at 14,000 rpm for 2 min. Total RNA was extracted using the RNeasy Mini Kit automated on QiaCube (Qiagen, Hilden, Germany). .. RNA from the corresponding supernatants was isolated using the QiaAmp Viral RNA kit automated on QiaCube.

    Amplification:

    Article Title: Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture
    Article Snippet: RNA was isolated with RNeasy Mini Kit (Qiagen) following manufacturer’s instructions. .. The amplified fragmented cDNA of 300 bp in size were sequenced in single-end mode using the Nova Seq 6000 (Illumina) with a read length of 100 bp.

    Synthesized:

    Article Title: Simultaneous targeting of XPO1 and BCL2 as an effective treatment strategy for double-hit lymphoma
    Article Snippet: RNA binding and purification were performed using the RNeasy Mini Kit (Qiagen). .. Total RNA was extracted with the RNeasy Mini Kit (Qiagen). cDNA was synthesized using the Verso cDNA Synthesis Kit (Thermo Scientific) according to the manufacturer’s instructions. qPCR analysis was performed on the 7500 Real-Time PCR System using SYBR Green (Applied Biosystems).

    Quantitative RT-PCR:

    Article Title: Roxatidine inhibits fibrosis by inhibiting NF-κB and MAPK signaling in macrophages sensing breast implant surface materials
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) Total RNA was extracted from RAW 264.7 and L929 cells using a commercial kit (RNeasy Mini kit; Qiagen GmbH) following the manufacturer's instructions. ..

    Article Title: A Novel ‘Candidatus Liberibacter asiaticus’-Encoded Sec-Dependent Secretory Protein Suppresses Programmed Cell Death in Nicotiana benthamiana
    Article Snippet: Paragraph title: 4.6. RT-qPCR Analysis ... At 48 hpi, the infiltrated leaf tissues were collected and pooled for total RNA extraction using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions.

    Real-time Polymerase Chain Reaction:

    Article Title: Respiratory syncytial virus exhibits differential tropism for distinct human placental cell types with Hofbauer cells acting as a permissive reservoir for infection
    Article Snippet: .. Quantitative polymerase chain reaction (qPCR) Cells were scraped and homogenized in RLT buffer with 1% betamercaptoethanol by vortexing and centrifugation through QiaShredder tubes at 14,000 rpm for 2 min. Total RNA was extracted using the RNeasy Mini Kit automated on QiaCube (Qiagen, Hilden, Germany). .. RNA from the corresponding supernatants was isolated using the QiaAmp Viral RNA kit automated on QiaCube.

    Article Title: Mechanosensitive transcriptional coactivators MRTF-A and YAP/TAZ regulate nucleus pulposus cell phenotype through cell shape
    Article Snippet: Paragraph title: mRNA extraction and real-time quantitative PCR ... Cells cultured on glass substrates with coatings were lysed by addition of RLT buffer with β-mercaptoethanol. mRNA extraction was performed on all samples using an RNeasy Mini Kit with DNase I Digestion (Qiagen).

    Article Title: Roxatidine inhibits fibrosis by inhibiting NF-κB and MAPK signaling in macrophages sensing breast implant surface materials
    Article Snippet: Reverse transcription-quantitative PCR (RT-qPCR) Total RNA was extracted from RAW 264.7 and L929 cells using a commercial kit (RNeasy Mini kit; Qiagen GmbH) following the manufacturer's instructions. .. Total RNA (2 µg) was reverse-transcribed to cDNA using an RT kit (High-Capacity cDNA Reverse Transcription kit, Thermo Fisher Scientific, Inc.), according to the manufacturer's manual. qPCR for target genes and the housekeeping gene GAPDH were performed using the SYBR® Green Master mix (Thermo Fisher Scientific, Inc.) following the manufacturer's instructions.

    Article Title: A Novel ‘Candidatus Liberibacter asiaticus’-Encoded Sec-Dependent Secretory Protein Suppresses Programmed Cell Death in Nicotiana benthamiana
    Article Snippet: At 48 hpi, the infiltrated leaf tissues were collected and pooled for total RNA extraction using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions. .. Immediately, total RNA was reverse-transcribed into first strand cDNA using the PrimeScript TM RT reagent Kit (TaKaRa, Kusatsu, Japan) with oligo (dT) as the primer. qPCR was performed on an ABI StepOne plus Real-time PCR instrument (Applied Biosystems, Foster City, CA, USA) with a 20 μL reaction system containing 5 μL of 2 X SYBR Premix Ex Taq (TaKaRa), 2 μmol/L of each gene-specific primers (listed in ), 0.5 μL of the cDNA sample, and 0.2 μL of Rox Reference Dye II (TaKaRa, Kusatsu, Japan).

    Article Title: A change in the zinc ion concentration reflects the maturation of insulin-producing cells generated from adipose-derived mesenchymal stem cells
    Article Snippet: Quantitative reverse transcription-polymerase chain reaction analyses Total RNA was extracted from cultured cells with an RNeasy Mini Kit (QIAGEN, Hilden, Germany), according to the manufacturer’s instructions. .. Quantitative reverse transcription-polymerase chain reaction analysis was performed using TaqMan Gene Expression Assays in 7500 real-time polymerase chain reaction system with StepOne Plus software (Applied Biosystems, Foster City, CA).

    Article Title: Simultaneous targeting of XPO1 and BCL2 as an effective treatment strategy for double-hit lymphoma
    Article Snippet: Paragraph title: Cellular fractionation and quantitative real-time PCR ... RNA binding and purification were performed using the RNeasy Mini Kit (Qiagen).

    Microarray:

    Article Title: Gene expression is associated with virulence in murine macrophages infected with Leptospira spp
    Article Snippet: RNA extraction and quantification Total RNA (n = 3/experimental group) was extracted from macrophages with RNeasy Mini Kit (Qiagen, USA) according to manufacturer’s instructions. .. All samples used for microarray analysis had a RIN of 10 (Quality data is available on our data descriptor)[ ].

    Article Title: Transcriptomic Analysis Links Eosinophilic Esophagitis and Atopic Dermatitis
    Article Snippet: RNA Extraction Total RNA was extracted from the skin with RNeasy mini kit (Qiagen) or from the primary cell culture RNAdvance Tissue kit (Agencourt), following the manufacturer's protocol. .. The extraction quality of RNA was assessed (RNA 6000 Nano kit, Agilent), and a minimum RNA integrity number (RIN) score of seven was the threshold for performing microarray analysis.

    Incubation:

    Article Title: Transcriptome analysis of human brain microvascular endothelial cells response to Neisseria meningitidis and its antigen MafA using RNA-seq
    Article Snippet: .. After incubation, mRNA from hBMECs was isolated using RNeasy Mini Kit (Qiagen, Germany) according to manufacturer’s instructions. .. Of note, DNaseI (Qiagen) treatment was essentially incorporated during RNA isolation.

    Expressing:

    Article Title: Targeting bromodomain-containing protein 4 (BRD4) inhibits MYC expression in colorectal cancer cells
    Article Snippet: .. RNA sequencing For global gene expression analysis, total RNA was isolated from cells using the RNeasy Mini Kit (Qiagen) with on-column digestion of DNA. .. Poly-A+ RNA was purified from total RNA with the NEBNextPoly(A) mRNA Magnetic Isolation Module (NEB).

    Article Title: Roxatidine inhibits fibrosis by inhibiting NF-κB and MAPK signaling in macrophages sensing breast implant surface materials
    Article Snippet: Reverse transcription-quantitative PCR (RT-qPCR) Total RNA was extracted from RAW 264.7 and L929 cells using a commercial kit (RNeasy Mini kit; Qiagen GmbH) following the manufacturer's instructions. .. Relative mRNA expression values were normalized according to levels of GAPDH.

    Article Title: Sarcosine promotes trafficking of dendritic cells and improves efficacy of anti-tumor dendritic cell vaccines via CXC chemokine family signaling
    Article Snippet: .. Gene expression RNA was extracted from murine myeloid DCs by RNeasy Mini Kit (QIAGEN). .. After reverse transcripting the extracted RNA, cDNA was implemented for gene expression analysis.

    Article Title: A Novel ‘Candidatus Liberibacter asiaticus’-Encoded Sec-Dependent Secretory Protein Suppresses Programmed Cell Death in Nicotiana benthamiana
    Article Snippet: At 48 hpi, the infiltrated leaf tissues were collected and pooled for total RNA extraction using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions. .. Finally, the 2-ΔΔCt method [ ] was utilized to calculate the relative gene expression values, which were subsequently transformed to fold-change and plotted in the figures.

    Article Title: A change in the zinc ion concentration reflects the maturation of insulin-producing cells generated from adipose-derived mesenchymal stem cells
    Article Snippet: Quantitative reverse transcription-polymerase chain reaction analyses Total RNA was extracted from cultured cells with an RNeasy Mini Kit (QIAGEN, Hilden, Germany), according to the manufacturer’s instructions. .. Quantitative reverse transcription-polymerase chain reaction analysis was performed using TaqMan Gene Expression Assays in 7500 real-time polymerase chain reaction system with StepOne Plus software (Applied Biosystems, Foster City, CA).

    Article Title: Simultaneous targeting of XPO1 and BCL2 as an effective treatment strategy for double-hit lymphoma
    Article Snippet: RNA binding and purification were performed using the RNeasy Mini Kit (Qiagen). .. Transcript levels were normalized to GAPDH, and relative gene expression was determined with ddCt method.

    Cell Fractionation:

    Article Title: Simultaneous targeting of XPO1 and BCL2 as an effective treatment strategy for double-hit lymphoma
    Article Snippet: Paragraph title: Cellular fractionation and quantitative real-time PCR ... RNA binding and purification were performed using the RNeasy Mini Kit (Qiagen).

    Transformation Assay:

    Article Title: A Novel ‘Candidatus Liberibacter asiaticus’-Encoded Sec-Dependent Secretory Protein Suppresses Programmed Cell Death in Nicotiana benthamiana
    Article Snippet: At 48 hpi, the infiltrated leaf tissues were collected and pooled for total RNA extraction using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions. .. Finally, the 2-ΔΔCt method [ ] was utilized to calculate the relative gene expression values, which were subsequently transformed to fold-change and plotted in the figures.

    RNA Binding Assay:

    Article Title: Simultaneous targeting of XPO1 and BCL2 as an effective treatment strategy for double-hit lymphoma
    Article Snippet: .. RNA binding and purification were performed using the RNeasy Mini Kit (Qiagen). .. Total RNA was extracted with the RNeasy Mini Kit (Qiagen). cDNA was synthesized using the Verso cDNA Synthesis Kit (Thermo Scientific) according to the manufacturer’s instructions. qPCR analysis was performed on the 7500 Real-Time PCR System using SYBR Green (Applied Biosystems).

    Cell Culture:

    Article Title: Transcriptomic Analysis Links Eosinophilic Esophagitis and Atopic Dermatitis
    Article Snippet: .. RNA Extraction Total RNA was extracted from the skin with RNeasy mini kit (Qiagen) or from the primary cell culture RNAdvance Tissue kit (Agencourt), following the manufacturer's protocol. .. The extraction quality of RNA was assessed (RNA 6000 Nano kit, Agilent), and a minimum RNA integrity number (RIN) score of seven was the threshold for performing microarray analysis.

    Article Title: Mechanosensitive transcriptional coactivators MRTF-A and YAP/TAZ regulate nucleus pulposus cell phenotype through cell shape
    Article Snippet: .. Cells cultured on glass substrates with coatings were lysed by addition of RLT buffer with β-mercaptoethanol. mRNA extraction was performed on all samples using an RNeasy Mini Kit with DNase I Digestion (Qiagen). .. Quality and concentration of mRNA was determined using a NanoDrop One Spectrophotometer (Thermo Fisher Scientific) to verify absorbance at 260 and 280 nm. mRNA was reverse transcribed into cDNA using an iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA).

    Article Title: Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture
    Article Snippet: Bulk 3′ RNA-sequencing For human pediatric SI organoids, RNA was isolated from cultured organoids in ECM gel and Matrigel with 20 min treatment of the droplets with Cell Recovery Solution (Corning) at 4 °C. .. RNA was isolated with RNeasy Mini Kit (Qiagen) following manufacturer’s instructions.

    Article Title: Transcriptome analysis of human brain microvascular endothelial cells response to Neisseria meningitidis and its antigen MafA using RNA-seq
    Article Snippet: Challenge of hBMECs hBMECs cultured in 6-well plates were incubated either with live NM (MOI 0.5, bacterial cells were washed with minimal essential medium, Sigma) or recombinant MafA (approximately 1 nMol, 27 μg/well) or non-related ligand of Streptococcus pneumoniae (as non-related protein control, approximately 1 nMol/well) or just the culture medium (non-induced control) for 6 hours at 37 °C in the presence of 5% CO2 . .. After incubation, mRNA from hBMECs was isolated using RNeasy Mini Kit (Qiagen, Germany) according to manufacturer’s instructions.

    Article Title: A change in the zinc ion concentration reflects the maturation of insulin-producing cells generated from adipose-derived mesenchymal stem cells
    Article Snippet: .. Quantitative reverse transcription-polymerase chain reaction analyses Total RNA was extracted from cultured cells with an RNeasy Mini Kit (QIAGEN, Hilden, Germany), according to the manufacturer’s instructions. .. RNA purity was assessed using a Nano Drop ND-1000 spectrometer (Thermo Scientific). cDNA was synthesised from total RNA using a reverse transcription kit (QIAGEN).

    Polymerase Chain Reaction:

    Article Title: Roxatidine inhibits fibrosis by inhibiting NF-κB and MAPK signaling in macrophages sensing breast implant surface materials
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) Total RNA was extracted from RAW 264.7 and L929 cells using a commercial kit (RNeasy Mini kit; Qiagen GmbH) following the manufacturer's instructions. ..

    Article Title: Sarcosine promotes trafficking of dendritic cells and improves efficacy of anti-tumor dendritic cell vaccines via CXC chemokine family signaling
    Article Snippet: Gene expression RNA was extracted from murine myeloid DCs by RNeasy Mini Kit (QIAGEN). .. PrimePCR Selections 96 well array plates were analyzed using Bio-Rad Reverse Transcription PCR (RT-PCR) machine (CFX96 Touch).

    Injection:

    Article Title: Inactivation of Interferon Regulatory Factor 1 Causes Susceptibility to Colitis-Associated Colorectal Cancer
    Article Snippet: RNA-sequencing Mice received one injection of AOM (D1) followed by one 4-day cycle of DSS (2%; D8-D11) and were sacrificed at D26. .. RNA was isolated from homogenized tissue with TRIzol reagent (Life Technologies) and purified using an RNeasy Mini Kit (Qiagen).

    Recombinant:

    Article Title: Transcriptome analysis of human brain microvascular endothelial cells response to Neisseria meningitidis and its antigen MafA using RNA-seq
    Article Snippet: Please note that recombinant non-related ligand of Streptococcus pneumoniae used here was produced using same conditions described for recombinant MafA (unpublished data). .. After incubation, mRNA from hBMECs was isolated using RNeasy Mini Kit (Qiagen, Germany) according to manufacturer’s instructions.

    RNA Sequencing Assay:

    Article Title: Targeting bromodomain-containing protein 4 (BRD4) inhibits MYC expression in colorectal cancer cells
    Article Snippet: .. RNA sequencing For global gene expression analysis, total RNA was isolated from cells using the RNeasy Mini Kit (Qiagen) with on-column digestion of DNA. .. Poly-A+ RNA was purified from total RNA with the NEBNextPoly(A) mRNA Magnetic Isolation Module (NEB).

    Article Title: Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture
    Article Snippet: Paragraph title: Bulk 3′ RNA-sequencing ... RNA was isolated with RNeasy Mini Kit (Qiagen) following manufacturer’s instructions.

    Article Title: Inactivation of Interferon Regulatory Factor 1 Causes Susceptibility to Colitis-Associated Colorectal Cancer
    Article Snippet: Paragraph title: RNA-sequencing ... RNA was isolated from homogenized tissue with TRIzol reagent (Life Technologies) and purified using an RNeasy Mini Kit (Qiagen).

    Isolation:

    Article Title: Targeting bromodomain-containing protein 4 (BRD4) inhibits MYC expression in colorectal cancer cells
    Article Snippet: .. RNA sequencing For global gene expression analysis, total RNA was isolated from cells using the RNeasy Mini Kit (Qiagen) with on-column digestion of DNA. .. Poly-A+ RNA was purified from total RNA with the NEBNextPoly(A) mRNA Magnetic Isolation Module (NEB).

    Article Title: Respiratory syncytial virus exhibits differential tropism for distinct human placental cell types with Hofbauer cells acting as a permissive reservoir for infection
    Article Snippet: Quantitative polymerase chain reaction (qPCR) Cells were scraped and homogenized in RLT buffer with 1% betamercaptoethanol by vortexing and centrifugation through QiaShredder tubes at 14,000 rpm for 2 min. Total RNA was extracted using the RNeasy Mini Kit automated on QiaCube (Qiagen, Hilden, Germany). .. RNA from the corresponding supernatants was isolated using the QiaAmp Viral RNA kit automated on QiaCube.

    Article Title: Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture
    Article Snippet: .. RNA was isolated with RNeasy Mini Kit (Qiagen) following manufacturer’s instructions. .. Total RNA (100 ng) from each sample was prepared using QuantSeq 3′ mRNA-Seq Library prep kit (Lexogen GmbH) according to manufacturer’s instructions.

    Article Title: Transcriptome analysis of human brain microvascular endothelial cells response to Neisseria meningitidis and its antigen MafA using RNA-seq
    Article Snippet: .. After incubation, mRNA from hBMECs was isolated using RNeasy Mini Kit (Qiagen, Germany) according to manufacturer’s instructions. .. Of note, DNaseI (Qiagen) treatment was essentially incorporated during RNA isolation.

    Article Title: Inactivation of Interferon Regulatory Factor 1 Causes Susceptibility to Colitis-Associated Colorectal Cancer
    Article Snippet: .. RNA was isolated from homogenized tissue with TRIzol reagent (Life Technologies) and purified using an RNeasy Mini Kit (Qiagen). .. RNA integrity was ascertained using a Bioanalyzer Total RNA Pico assay (Agilent). rRNA-depleted libraries were prepared using Kapa-stranded RNA-seq kit (Kapa Biosystems), sequenced as previously described and analyzed using publicly available bioinformatics work tools and software packages – .

    Article Title: Simultaneous targeting of XPO1 and BCL2 as an effective treatment strategy for double-hit lymphoma
    Article Snippet: Cellular fractionation and quantitative real-time PCR Cytoplasmic and nuclear RNA were isolated and purified using the RNA Subcellular Isolation Kit (Active Motif) following the manufacturer’s protocol. .. RNA binding and purification were performed using the RNeasy Mini Kit (Qiagen).

    Purification:

    Article Title: Targeting bromodomain-containing protein 4 (BRD4) inhibits MYC expression in colorectal cancer cells
    Article Snippet: RNA sequencing For global gene expression analysis, total RNA was isolated from cells using the RNeasy Mini Kit (Qiagen) with on-column digestion of DNA. .. Poly-A+ RNA was purified from total RNA with the NEBNextPoly(A) mRNA Magnetic Isolation Module (NEB).

    Article Title: Inactivation of Interferon Regulatory Factor 1 Causes Susceptibility to Colitis-Associated Colorectal Cancer
    Article Snippet: .. RNA was isolated from homogenized tissue with TRIzol reagent (Life Technologies) and purified using an RNeasy Mini Kit (Qiagen). .. RNA integrity was ascertained using a Bioanalyzer Total RNA Pico assay (Agilent). rRNA-depleted libraries were prepared using Kapa-stranded RNA-seq kit (Kapa Biosystems), sequenced as previously described and analyzed using publicly available bioinformatics work tools and software packages – .

    Article Title: Simultaneous targeting of XPO1 and BCL2 as an effective treatment strategy for double-hit lymphoma
    Article Snippet: .. RNA binding and purification were performed using the RNeasy Mini Kit (Qiagen). .. Total RNA was extracted with the RNeasy Mini Kit (Qiagen). cDNA was synthesized using the Verso cDNA Synthesis Kit (Thermo Scientific) according to the manufacturer’s instructions. qPCR analysis was performed on the 7500 Real-Time PCR System using SYBR Green (Applied Biosystems).

    Article Title: Expression of serotonin 2A, 2C, 6 and 7 receptor and IL-6 mRNA in experimental toxoplasmic encephalitis in mice
    Article Snippet: .. The total RNA was purified using RNeasy Mini Kit Qiagen according to the manufacturer's instructions in Qiaqube (Qiagen). .. The RNA samples were reverse-transcribed into complementary DNA by the High Capacity cDNA Reverse Transcription Kit (Applied Biosystem).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Sarcosine promotes trafficking of dendritic cells and improves efficacy of anti-tumor dendritic cell vaccines via CXC chemokine family signaling
    Article Snippet: Gene expression RNA was extracted from murine myeloid DCs by RNeasy Mini Kit (QIAGEN). .. PrimePCR Selections 96 well array plates were analyzed using Bio-Rad Reverse Transcription PCR (RT-PCR) machine (CFX96 Touch).

    Article Title: A change in the zinc ion concentration reflects the maturation of insulin-producing cells generated from adipose-derived mesenchymal stem cells
    Article Snippet: .. Quantitative reverse transcription-polymerase chain reaction analyses Total RNA was extracted from cultured cells with an RNeasy Mini Kit (QIAGEN, Hilden, Germany), according to the manufacturer’s instructions. .. RNA purity was assessed using a Nano Drop ND-1000 spectrometer (Thermo Scientific). cDNA was synthesised from total RNA using a reverse transcription kit (QIAGEN).

    Mouse Assay:

    Article Title: Inactivation of Interferon Regulatory Factor 1 Causes Susceptibility to Colitis-Associated Colorectal Cancer
    Article Snippet: RNA-sequencing Mice received one injection of AOM (D1) followed by one 4-day cycle of DSS (2%; D8-D11) and were sacrificed at D26. .. RNA was isolated from homogenized tissue with TRIzol reagent (Life Technologies) and purified using an RNeasy Mini Kit (Qiagen).

    Chromatin Immunoprecipitation:

    Article Title: Targeting bromodomain-containing protein 4 (BRD4) inhibits MYC expression in colorectal cancer cells
    Article Snippet: RNA sequencing For global gene expression analysis, total RNA was isolated from cells using the RNeasy Mini Kit (Qiagen) with on-column digestion of DNA. .. Library quality and concentration were determined using the Experion chip electrophoresis system (Bio-Rad).

    Software:

    Article Title: Inactivation of Interferon Regulatory Factor 1 Causes Susceptibility to Colitis-Associated Colorectal Cancer
    Article Snippet: RNA was isolated from homogenized tissue with TRIzol reagent (Life Technologies) and purified using an RNeasy Mini Kit (Qiagen). .. RNA integrity was ascertained using a Bioanalyzer Total RNA Pico assay (Agilent). rRNA-depleted libraries were prepared using Kapa-stranded RNA-seq kit (Kapa Biosystems), sequenced as previously described and analyzed using publicly available bioinformatics work tools and software packages – .

    Article Title: A change in the zinc ion concentration reflects the maturation of insulin-producing cells generated from adipose-derived mesenchymal stem cells
    Article Snippet: Quantitative reverse transcription-polymerase chain reaction analyses Total RNA was extracted from cultured cells with an RNeasy Mini Kit (QIAGEN, Hilden, Germany), according to the manufacturer’s instructions. .. Quantitative reverse transcription-polymerase chain reaction analysis was performed using TaqMan Gene Expression Assays in 7500 real-time polymerase chain reaction system with StepOne Plus software (Applied Biosystems, Foster City, CA).

    SYBR Green Assay:

    Article Title: Roxatidine inhibits fibrosis by inhibiting NF-κB and MAPK signaling in macrophages sensing breast implant surface materials
    Article Snippet: Reverse transcription-quantitative PCR (RT-qPCR) Total RNA was extracted from RAW 264.7 and L929 cells using a commercial kit (RNeasy Mini kit; Qiagen GmbH) following the manufacturer's instructions. .. Total RNA (2 µg) was reverse-transcribed to cDNA using an RT kit (High-Capacity cDNA Reverse Transcription kit, Thermo Fisher Scientific, Inc.), according to the manufacturer's manual. qPCR for target genes and the housekeeping gene GAPDH were performed using the SYBR® Green Master mix (Thermo Fisher Scientific, Inc.) following the manufacturer's instructions.

    Article Title: Simultaneous targeting of XPO1 and BCL2 as an effective treatment strategy for double-hit lymphoma
    Article Snippet: RNA binding and purification were performed using the RNeasy Mini Kit (Qiagen). .. Total RNA was extracted with the RNeasy Mini Kit (Qiagen). cDNA was synthesized using the Verso cDNA Synthesis Kit (Thermo Scientific) according to the manufacturer’s instructions. qPCR analysis was performed on the 7500 Real-Time PCR System using SYBR Green (Applied Biosystems).

    RNA Extraction:

    Article Title: Gene expression is associated with virulence in murine macrophages infected with Leptospira spp
    Article Snippet: .. RNA extraction and quantification Total RNA (n = 3/experimental group) was extracted from macrophages with RNeasy Mini Kit (Qiagen, USA) according to manufacturer’s instructions. ..

    Article Title: Transcriptomic Analysis Links Eosinophilic Esophagitis and Atopic Dermatitis
    Article Snippet: .. RNA Extraction Total RNA was extracted from the skin with RNeasy mini kit (Qiagen) or from the primary cell culture RNAdvance Tissue kit (Agencourt), following the manufacturer's protocol. .. The extraction quality of RNA was assessed (RNA 6000 Nano kit, Agilent), and a minimum RNA integrity number (RIN) score of seven was the threshold for performing microarray analysis.

    Article Title: A Novel ‘Candidatus Liberibacter asiaticus’-Encoded Sec-Dependent Secretory Protein Suppresses Programmed Cell Death in Nicotiana benthamiana
    Article Snippet: .. At 48 hpi, the infiltrated leaf tissues were collected and pooled for total RNA extraction using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions. .. Immediately, total RNA was reverse-transcribed into first strand cDNA using the PrimeScript TM RT reagent Kit (TaKaRa, Kusatsu, Japan) with oligo (dT) as the primer. qPCR was performed on an ABI StepOne plus Real-time PCR instrument (Applied Biosystems, Foster City, CA, USA) with a 20 μL reaction system containing 5 μL of 2 X SYBR Premix Ex Taq (TaKaRa), 2 μmol/L of each gene-specific primers (listed in ), 0.5 μL of the cDNA sample, and 0.2 μL of Rox Reference Dye II (TaKaRa, Kusatsu, Japan).

    Article Title: Expression Quantitative Trait Loci in Equine Skeletal Muscle Reveals Heritable Variation in Metabolism and the Training Responsive Transcriptome
    Article Snippet: .. RNA Extraction and Quality Control Total RNA was extracted from approximately 70 mg tissue using a protocol combining TRIzol reagent (Thermo Fisher), DNase I treatment (Qiagen, Hilden, Germany) and an RNeasy Mini-Kit (Qiagen). .. RNA was quantified using a Nano Drop ND1000 spectrophotometer V 3.5.27 (Thermo Fisher).

    Article Title: Expression of serotonin 2A, 2C, 6 and 7 receptor and IL-6 mRNA in experimental toxoplasmic encephalitis in mice
    Article Snippet: Paragraph title: Total RNA extraction and cDNA synthesis ... The total RNA was purified using RNeasy Mini Kit Qiagen according to the manufacturer's instructions in Qiaqube (Qiagen).

    Binding Assay:

    Article Title: Transcriptome analysis of human brain microvascular endothelial cells response to Neisseria meningitidis and its antigen MafA using RNA-seq
    Article Snippet: This non-related ligand is one of the endothelial cell binding proteins of S. pneumoniae described in our previous publication . .. After incubation, mRNA from hBMECs was isolated using RNeasy Mini Kit (Qiagen, Germany) according to manufacturer’s instructions.

    Electrophoresis:

    Article Title: Targeting bromodomain-containing protein 4 (BRD4) inhibits MYC expression in colorectal cancer cells
    Article Snippet: RNA sequencing For global gene expression analysis, total RNA was isolated from cells using the RNeasy Mini Kit (Qiagen) with on-column digestion of DNA. .. Library quality and concentration were determined using the Experion chip electrophoresis system (Bio-Rad).

    Article Title: Gene expression is associated with virulence in murine macrophages infected with Leptospira spp
    Article Snippet: RNA extraction and quantification Total RNA (n = 3/experimental group) was extracted from macrophages with RNeasy Mini Kit (Qiagen, USA) according to manufacturer’s instructions. .. The quantification was performed using a NanoDrop (ND-2000 spectrophotometer, Thermo Scientific, Wilmington, DE, USA) and the samples quality was assessed using capillary electrophoresis (Bioanalyzer 2100 Agilent, Santa Clara, CA, USA).

    Article Title: Transcriptome analysis of human brain microvascular endothelial cells response to Neisseria meningitidis and its antigen MafA using RNA-seq
    Article Snippet: After incubation, mRNA from hBMECs was isolated using RNeasy Mini Kit (Qiagen, Germany) according to manufacturer’s instructions. .. Integrity of RNA was monitored using a capillary electrophoresis (Fragment analyzer, Advanced Analytical Technologies, Inc, USA).

    Spectrophotometry:

    Article Title: Gene expression is associated with virulence in murine macrophages infected with Leptospira spp
    Article Snippet: RNA extraction and quantification Total RNA (n = 3/experimental group) was extracted from macrophages with RNeasy Mini Kit (Qiagen, USA) according to manufacturer’s instructions. .. The quantification was performed using a NanoDrop (ND-2000 spectrophotometer, Thermo Scientific, Wilmington, DE, USA) and the samples quality was assessed using capillary electrophoresis (Bioanalyzer 2100 Agilent, Santa Clara, CA, USA).

    Article Title: Mechanosensitive transcriptional coactivators MRTF-A and YAP/TAZ regulate nucleus pulposus cell phenotype through cell shape
    Article Snippet: Cells cultured on glass substrates with coatings were lysed by addition of RLT buffer with β-mercaptoethanol. mRNA extraction was performed on all samples using an RNeasy Mini Kit with DNase I Digestion (Qiagen). .. Quality and concentration of mRNA was determined using a NanoDrop One Spectrophotometer (Thermo Fisher Scientific) to verify absorbance at 260 and 280 nm. mRNA was reverse transcribed into cDNA using an iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA).

    Article Title: Expression Quantitative Trait Loci in Equine Skeletal Muscle Reveals Heritable Variation in Metabolism and the Training Responsive Transcriptome
    Article Snippet: RNA Extraction and Quality Control Total RNA was extracted from approximately 70 mg tissue using a protocol combining TRIzol reagent (Thermo Fisher), DNase I treatment (Qiagen, Hilden, Germany) and an RNeasy Mini-Kit (Qiagen). .. RNA was quantified using a Nano Drop ND1000 spectrophotometer V 3.5.27 (Thermo Fisher).

    Produced:

    Article Title: Transcriptome analysis of human brain microvascular endothelial cells response to Neisseria meningitidis and its antigen MafA using RNA-seq
    Article Snippet: Please note that recombinant non-related ligand of Streptococcus pneumoniae used here was produced using same conditions described for recombinant MafA (unpublished data). .. After incubation, mRNA from hBMECs was isolated using RNeasy Mini Kit (Qiagen, Germany) according to manufacturer’s instructions.

    Concentration Assay:

    Article Title: Targeting bromodomain-containing protein 4 (BRD4) inhibits MYC expression in colorectal cancer cells
    Article Snippet: RNA sequencing For global gene expression analysis, total RNA was isolated from cells using the RNeasy Mini Kit (Qiagen) with on-column digestion of DNA. .. Library quality and concentration were determined using the Experion chip electrophoresis system (Bio-Rad).

    Article Title: Mechanosensitive transcriptional coactivators MRTF-A and YAP/TAZ regulate nucleus pulposus cell phenotype through cell shape
    Article Snippet: Cells cultured on glass substrates with coatings were lysed by addition of RLT buffer with β-mercaptoethanol. mRNA extraction was performed on all samples using an RNeasy Mini Kit with DNase I Digestion (Qiagen). .. Quality and concentration of mRNA was determined using a NanoDrop One Spectrophotometer (Thermo Fisher Scientific) to verify absorbance at 260 and 280 nm. mRNA was reverse transcribed into cDNA using an iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA).

    Article Title: Transcriptome analysis of human brain microvascular endothelial cells response to Neisseria meningitidis and its antigen MafA using RNA-seq
    Article Snippet: After incubation, mRNA from hBMECs was isolated using RNeasy Mini Kit (Qiagen, Germany) according to manufacturer’s instructions. .. The RNA concentration was measured on nanodrop (Thermo scientific) and it was stored at −80 °C in aliquots.

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    Qiagen rneasy mini kit
    Rneasy Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 8038 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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