rneasy plus micro kit 74034  (Qiagen)


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    Name:
    RNeasy Plus Micro Kit
    Description:
    For purification of up to 45 µg total RNA from cells tissues using gDNA Eliminator columns Kit contents Qiagen RNeasy Plus Micro Kit 50 preps 14L Elution Volume 5mg Tissue Amount Tissue Cells Sample Total RNA Purification Spin Column Format Silica Technology Ideal for Northern Dot and Slot Blotting End point RT PCR Quantitative Real time RT PCR Includes RNeasy MinElute Spin Columns gDNA Eliminator Spin Columns Collection Tubes Carrier RNA RNase free Water and Buffers Benefits Unique gDNA Eliminator columns avoid the need for DNase Efficient removal of genomic DNA Highly reproducible yields of RNA in minutes High performance RNA for sensitive application
    Catalog Number:
    74034
    Price:
    436
    Category:
    RNeasy Plus Micro Kit
    Buy from Supplier


    Structured Review

    Qiagen rneasy plus micro kit 74034
    RNeasy Plus Micro Kit
    For purification of up to 45 µg total RNA from cells tissues using gDNA Eliminator columns Kit contents Qiagen RNeasy Plus Micro Kit 50 preps 14L Elution Volume 5mg Tissue Amount Tissue Cells Sample Total RNA Purification Spin Column Format Silica Technology Ideal for Northern Dot and Slot Blotting End point RT PCR Quantitative Real time RT PCR Includes RNeasy MinElute Spin Columns gDNA Eliminator Spin Columns Collection Tubes Carrier RNA RNase free Water and Buffers Benefits Unique gDNA Eliminator columns avoid the need for DNase Efficient removal of genomic DNA Highly reproducible yields of RNA in minutes High performance RNA for sensitive application
    https://www.bioz.com/result/rneasy plus micro kit 74034/product/Qiagen
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    rneasy plus micro kit 74034 - by Bioz Stars, 2020-01
    90/100 stars

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    Related Articles

    Amplification:

    Article Title: The OV-TL 12/30 Clone of Anti-cytokeratin 7 Antibody as a New Marker of Corneal Conjunctivalization in Patients with Limbal Stem Cell Deficiency
    Article Snippet: Total RNA was extracted from imprints of the central corneal epithelium and upper bulbar conjunctival epithelium of six cadaveric bulbi (RNeasy Plus Micro Kit; Qiagen, Hilden, Germany). .. Subsequently, individual samples were amplified with the following specific oligonucleotides for CK7 and the housekeeping gene glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ; synthesized by Generi Biotech, Hradec Králové, Czech Republic): human CK7, sense primer 5′-cag gac cct caa tga gacg-3′, antisense primer 5′-cca ggg agc gac tgt tgt-3′; and human GAPDH , sense primer 5′-AGC CAC ATC GCT CAG ACAC-3′, antisense primer 5′-GCC CAA TAC GAC CAA ATCC-3′.

    Article Title: Effects of Controlled Cortical Impact on the Mouse Brain Vasculome
    Article Snippet: Total RNA was extracted from the fresh preparation of brain microvasculature with the RNeasy Plus Micro kit (Qiagen, Hilden, Germany). .. Microarray hybridization and scanning was done by Expression Analysis Corporation with Affymetrix murine whole genome microarray M430 2.0, with 3-IVT ( in vitro transcription) and Encore Biotin Module for amplification, fragmentation, and labeling.

    Article Title: Stellate Cells, Hepatocytes, and Endothelial Cells Imprint the Kupffer Cell Identity on Monocytes Colonizing the Liver Macrophage Niche
    Article Snippet: Gene expression analysis by real-time RT-PCR RNA was purified from 2,5x104 sorted cells using a RNeasy Plus micro kit (QIAGEN). .. Gene expression was assayed by real-time RT-PCR using a SensiFast SYBR No-Rox kit (Bioline) on a PCR amplification and detection instrument (LightCycler 480; Roche) with the primers listed in the .

    Article Title: Obesity, Diabetes and Energy Homeostasis: Reduced uterine perfusion pressure causes loss of pancreatic β-cell area but normal function in fetal rat offspring
    Article Snippet: After an overnight incubation, fetal islets intended for RNA isolation were stored at −80°C in 20 μl RNAlater solution (Invitrogen) and subsequently isolated using the RNeasy Plus Micro Kit (Qiagen), according to the manufacturer’s protocol. .. Amplification was carried out using Power SYBR Green PCR Master Mix in QuantStudio Flex Real-Time PCR System for 40 cycles (Applied Biosystems).

    Article Title: Glutamine Stimulates Biosynthesis and Secretion of Insulin-like Growth Factor 2 (IGF2), an Autocrine Regulator of Beta Cell Mass and Function *
    Article Snippet: Total RNA was extracted using RNeasy Plus Micro Kit from Qiagen, and cDNAs were synthesized using SuperScript II RNase H reverse transcriptase (Invitrogen), and 50 pmol of random hexamers (Applied Biosystems, Basel, Switzerland). .. Amplification was performed in a 20-μl reaction mixture including 1× QuantiTectTM SYBR Green PCR Master Mix (Qiagen) and 10 pmol of each primer.

    Synthesized:

    Article Title: The OV-TL 12/30 Clone of Anti-cytokeratin 7 Antibody as a New Marker of Corneal Conjunctivalization in Patients with Limbal Stem Cell Deficiency
    Article Snippet: Total RNA was extracted from imprints of the central corneal epithelium and upper bulbar conjunctival epithelium of six cadaveric bulbi (RNeasy Plus Micro Kit; Qiagen, Hilden, Germany). .. Subsequently, individual samples were amplified with the following specific oligonucleotides for CK7 and the housekeeping gene glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ; synthesized by Generi Biotech, Hradec Králové, Czech Republic): human CK7, sense primer 5′-cag gac cct caa tga gacg-3′, antisense primer 5′-cca ggg agc gac tgt tgt-3′; and human GAPDH , sense primer 5′-AGC CAC ATC GCT CAG ACAC-3′, antisense primer 5′-GCC CAA TAC GAC CAA ATCC-3′.

    Article Title: Glutamine Stimulates Biosynthesis and Secretion of Insulin-like Growth Factor 2 (IGF2), an Autocrine Regulator of Beta Cell Mass and Function *
    Article Snippet: .. Total RNA was extracted using RNeasy Plus Micro Kit from Qiagen, and cDNAs were synthesized using SuperScript II RNase H reverse transcriptase (Invitrogen), and 50 pmol of random hexamers (Applied Biosystems, Basel, Switzerland). .. Real time quantitative PCR was performed using a 7500 Fast Light Cycler technology Roche Applied Science (Basel, Switzerland).

    Quantitative RT-PCR:

    Article Title: Intestinal microbiota sustains inflammation and autoimmunity induced by hypomorphic RAG defects
    Article Snippet: Quantitative RT-PCR was performed on a CFX384 system (Bio-Rad Laboratories) with the SYBR green master mix (Thermo Fisher Scientific). .. For sorted cells, total RNA was extracted using the RNeasy Plus Micro kit (QIAGEN).

    Article Title: Effects of Stress and Motivation on Performing a Spatial Task
    Article Snippet: .. Total RNA was extracted from the microdissected brain areas (Qiagen RNeasy Plus Micro Kit; Alameda, CA), cDNA synthesis was conducted (BioRad iScript cDNA; Alameda, CA), and quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to quantify mRNA expression in each region of the brain. .. The qRT-PCR protocol was based on procedures previously performed in our lab ( ; ).

    Article Title: Stellate Cells, Hepatocytes, and Endothelial Cells Imprint the Kupffer Cell Identity on Monocytes Colonizing the Liver Macrophage Niche
    Article Snippet: .. Gene expression analysis by real-time RT-PCR RNA was purified from 2,5x104 sorted cells using a RNeasy Plus micro kit (QIAGEN). .. RNA was reverse transcribed to cDNA with a Sensifast cDNA synthesis kit (Bioline).

    SYBR Green Assay:

    Article Title: Intestinal microbiota sustains inflammation and autoimmunity induced by hypomorphic RAG defects
    Article Snippet: Quantitative RT-PCR was performed on a CFX384 system (Bio-Rad Laboratories) with the SYBR green master mix (Thermo Fisher Scientific). .. For sorted cells, total RNA was extracted using the RNeasy Plus Micro kit (QIAGEN).

    Article Title: miR-219–5p Modulates Cell Growth of Papillary Thyroid Carcinoma by Targeting Estrogen Receptor α
    Article Snippet: Total RNA was isolated from tissue samples and cells using the RNeasy Plus Micro Kit (QIAGEN). .. For detection of the expression of ERα, mRNA was reverse transcribed using PrimeScript reverse transcriptase and random primers (TaKaRa), followed by qPCR with SYBR Green chemistry (Applied Biosystems) with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as an endogenous control.

    Article Title: Obesity, Diabetes and Energy Homeostasis: Reduced uterine perfusion pressure causes loss of pancreatic β-cell area but normal function in fetal rat offspring
    Article Snippet: After an overnight incubation, fetal islets intended for RNA isolation were stored at −80°C in 20 μl RNAlater solution (Invitrogen) and subsequently isolated using the RNeasy Plus Micro Kit (Qiagen), according to the manufacturer’s protocol. .. Amplification was carried out using Power SYBR Green PCR Master Mix in QuantStudio Flex Real-Time PCR System for 40 cycles (Applied Biosystems).

    Article Title: Glutamine Stimulates Biosynthesis and Secretion of Insulin-like Growth Factor 2 (IGF2), an Autocrine Regulator of Beta Cell Mass and Function *
    Article Snippet: Total RNA was extracted using RNeasy Plus Micro Kit from Qiagen, and cDNAs were synthesized using SuperScript II RNase H reverse transcriptase (Invitrogen), and 50 pmol of random hexamers (Applied Biosystems, Basel, Switzerland). .. Amplification was performed in a 20-μl reaction mixture including 1× QuantiTectTM SYBR Green PCR Master Mix (Qiagen) and 10 pmol of each primer.

    Microarray:

    Article Title: Effects of Controlled Cortical Impact on the Mouse Brain Vasculome
    Article Snippet: Paragraph title: Microarray sample analysis ... Total RNA was extracted from the fresh preparation of brain microvasculature with the RNeasy Plus Micro kit (Qiagen, Hilden, Germany).

    Article Title: Ablation of Kcnj10 expression in retinal explants revealed pivotal roles for Kcnj10 in the proliferation and development of Müller glia
    Article Snippet: Paragraph title: RNA-sequencing and microarray ... Total RNA was extracted from the retinas of wild-type C57/BL/6J mice at P7 or P14 using the RNeasy Plus Micro Kit (Qiagen), and the RNA quality was confirmed using a 2100 Bioanalyzer (Agilent Technologies).

    Quantitation Assay:

    Article Title: miR-219–5p Modulates Cell Growth of Papillary Thyroid Carcinoma by Targeting Estrogen Receptor α
    Article Snippet: Total RNA was isolated from tissue samples and cells using the RNeasy Plus Micro Kit (QIAGEN). .. Quantitation of U6 was performed as an endogenous control.

    Expressing:

    Article Title: Effects of Controlled Cortical Impact on the Mouse Brain Vasculome
    Article Snippet: Total RNA was extracted from the fresh preparation of brain microvasculature with the RNeasy Plus Micro kit (Qiagen, Hilden, Germany). .. Microarray hybridization and scanning was done by Expression Analysis Corporation with Affymetrix murine whole genome microarray M430 2.0, with 3-IVT ( in vitro transcription) and Encore Biotin Module for amplification, fragmentation, and labeling.

    Article Title: Follicular regulatory T cells inhibit the development of granzyme B–expressing follicular helper T cells
    Article Snippet: Paragraph title: Cell sorting, RNA isolation, and gene expression analysis. ... Total RNA was collected from freshly sorted cells using the RNeasy Plus Micro kit (QIAGEN). qPCR reactions were performed as previously reported ( ).

    Article Title: The Transferrin Receptor CD71 Delineates Functionally Distinct Airway Macrophage Subsets during Idiopathic Pulmonary Fibrosis
    Article Snippet: Real-Time PCR RNA extraction was performed using the RNeasy Plus Micro Kit (Qiagen) according to the manufacturer’s protocol. .. Gene expression was analyzed using the change-in-threshold comparative cycle threshold method.

    Article Title: The Telomerase Complex Directly Controls Hematopoietic Stem Cell Differentiation and Senescence in an Induced Pluripotent Stem Cell Model of Telomeropathy
    Article Snippet: .. Gene Expression Assay Genomic DNA and RNA were isolated by column separation using a DNeasy Blood & Tissue Kit (Qiagen 69504) and RNeasy Plus Micro Kit with gDNA elimination (Qiagen 74034), respectively. .. All qPCR assays were run using TaqManprobes and TaqManTM Gene Expression Master Mix (Invitrogen 4369016) in a Roche Lightcycler 480.

    Article Title: Effects of Stress and Motivation on Performing a Spatial Task
    Article Snippet: .. Total RNA was extracted from the microdissected brain areas (Qiagen RNeasy Plus Micro Kit; Alameda, CA), cDNA synthesis was conducted (BioRad iScript cDNA; Alameda, CA), and quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to quantify mRNA expression in each region of the brain. .. The qRT-PCR protocol was based on procedures previously performed in our lab ( ; ).

    Article Title: Stellate Cells, Hepatocytes, and Endothelial Cells Imprint the Kupffer Cell Identity on Monocytes Colonizing the Liver Macrophage Niche
    Article Snippet: .. Gene expression analysis by real-time RT-PCR RNA was purified from 2,5x104 sorted cells using a RNeasy Plus micro kit (QIAGEN). .. RNA was reverse transcribed to cDNA with a Sensifast cDNA synthesis kit (Bioline).

    Article Title: CD38 Inhibits Prostate Cancer Metabolism and Proliferation by Reducing Cellular NAD+ Pools
    Article Snippet: LNCaP cells transfected with either an empty vector or CD38 expression construct were plated (5 × 105 cells/dish) in 10cm dishes and treated with doxycycline (1μg/ml) for 96Hrs (N = 5 per group). .. Total RNA was purified from cell lines using the RNeasy Plus Micro Kit (Qiagen, Germantown, MD, USA) with genomic DNA removal.

    Hybridization:

    Article Title: Effects of Controlled Cortical Impact on the Mouse Brain Vasculome
    Article Snippet: Total RNA was extracted from the fresh preparation of brain microvasculature with the RNeasy Plus Micro kit (Qiagen, Hilden, Germany). .. Microarray hybridization and scanning was done by Expression Analysis Corporation with Affymetrix murine whole genome microarray M430 2.0, with 3-IVT ( in vitro transcription) and Encore Biotin Module for amplification, fragmentation, and labeling.

    Transfection:

    Article Title: CD38 Inhibits Prostate Cancer Metabolism and Proliferation by Reducing Cellular NAD+ Pools
    Article Snippet: LNCaP cells transfected with either an empty vector or CD38 expression construct were plated (5 × 105 cells/dish) in 10cm dishes and treated with doxycycline (1μg/ml) for 96Hrs (N = 5 per group). .. Total RNA was purified from cell lines using the RNeasy Plus Micro Kit (Qiagen, Germantown, MD, USA) with genomic DNA removal.

    Concentration Assay:

    Article Title: Effects of Controlled Cortical Impact on the Mouse Brain Vasculome
    Article Snippet: Total RNA was extracted from the fresh preparation of brain microvasculature with the RNeasy Plus Micro kit (Qiagen, Hilden, Germany). .. Concentration of RNA was measured by Nanodrop, and integrity of RNA was tested with the RNA integrity number (RIN) score on the Agilent Bioanalyzer 2100 (Agilent Technologies, Inc., Santa Clara, CAQ).

    Article Title: Transcriptomic Profiling of Zebrafish Hair Cells Using RiboTag
    Article Snippet: After washing, 350 μL of buffer RLT from the RNeasy Plus Micro kit (Qiagen) was added to the bound beads or reserved input sample and vortexed for 30 s to dissociate bound RNA. .. RNA quality and concentration was assessed using the Agilent RNA Pico kit (Agilent Technologies) at the UMSOM Genomics Core Facility.

    Protease Inhibitor:

    Article Title: Transcriptomic Profiling of Zebrafish Hair Cells Using RiboTag
    Article Snippet: Larvae were resuspended and homogenized in 1 mL of supplemented homogenization buffer (50 mM Tris-HCl pH.7, 100 mM KCl, 12 mM MgCl2 , 1% Nonidet P-40, 1 mM 1,4-Dithiothreitol, 1X protease inhibitor cocktail, 200 U/mL RNAsin, 100 μg/mL cycloheximide, 1 mg/mL heparin) by douncing on ice. .. After washing, 350 μL of buffer RLT from the RNeasy Plus Micro kit (Qiagen) was added to the bound beads or reserved input sample and vortexed for 30 s to dissociate bound RNA.

    Dissection:

    Article Title: Expression profiles of stress-related genes in islets from donors with progressively impaired glucose metabolism
    Article Snippet: Paragraph title: Laser capture micro dissection and RNA extraction ... RNA extraction was performed using a Qiagen RNeasy Plus Micro kit (Qiagen) according to the manufacturer's instructions.

    TaqMan microRNA Assay:

    Article Title: miR-219–5p Modulates Cell Growth of Papillary Thyroid Carcinoma by Targeting Estrogen Receptor α
    Article Snippet: Total RNA was isolated from tissue samples and cells using the RNeasy Plus Micro Kit (QIAGEN). .. For the detection of mature miRNA, miRNA was reverse transcribed using the TaqMan miRNA Reverse Transcription Kit (Applied Biosystems). qPCR involved the appropriate TaqMan miRNA assay (Applied Biosystems) and a Prism 7500 instrument (Applied Biosystems).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: The OV-TL 12/30 Clone of Anti-cytokeratin 7 Antibody as a New Marker of Corneal Conjunctivalization in Patients with Limbal Stem Cell Deficiency
    Article Snippet: Paragraph title: Semiquantitative Reverse Transcription–Polymerase Chain Reaction ... Total RNA was extracted from imprints of the central corneal epithelium and upper bulbar conjunctival epithelium of six cadaveric bulbi (RNeasy Plus Micro Kit; Qiagen, Hilden, Germany).

    Article Title: Intestinal microbiota sustains inflammation and autoimmunity induced by hypomorphic RAG defects
    Article Snippet: Paragraph title: RT-PCR ... For sorted cells, total RNA was extracted using the RNeasy Plus Micro kit (QIAGEN).

    Article Title: Effects of Stress and Motivation on Performing a Spatial Task
    Article Snippet: .. Total RNA was extracted from the microdissected brain areas (Qiagen RNeasy Plus Micro Kit; Alameda, CA), cDNA synthesis was conducted (BioRad iScript cDNA; Alameda, CA), and quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to quantify mRNA expression in each region of the brain. .. The qRT-PCR protocol was based on procedures previously performed in our lab ( ; ).

    Cellular Antioxidant Activity Assay:

    Article Title: The OV-TL 12/30 Clone of Anti-cytokeratin 7 Antibody as a New Marker of Corneal Conjunctivalization in Patients with Limbal Stem Cell Deficiency
    Article Snippet: Total RNA was extracted from imprints of the central corneal epithelium and upper bulbar conjunctival epithelium of six cadaveric bulbi (RNeasy Plus Micro Kit; Qiagen, Hilden, Germany). .. Subsequently, individual samples were amplified with the following specific oligonucleotides for CK7 and the housekeeping gene glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ; synthesized by Generi Biotech, Hradec Králové, Czech Republic): human CK7, sense primer 5′-cag gac cct caa tga gacg-3′, antisense primer 5′-cca ggg agc gac tgt tgt-3′; and human GAPDH , sense primer 5′-AGC CAC ATC GCT CAG ACAC-3′, antisense primer 5′-GCC CAA TAC GAC CAA ATCC-3′.

    Magnetic Cell Separation:

    Article Title: Follicular regulatory T cells inhibit the development of granzyme B–expressing follicular helper T cells
    Article Snippet: On day 36 after PCT challenge, CD4+ T cells were isolated from the spleen from mice using a CD4 T cell Macs isolation kit (Miltenyi Biotec). .. Total RNA was collected from freshly sorted cells using the RNeasy Plus Micro kit (QIAGEN). qPCR reactions were performed as previously reported ( ).

    RNA Sequencing Assay:

    Article Title: Ablation of Kcnj10 expression in retinal explants revealed pivotal roles for Kcnj10 in the proliferation and development of Müller glia
    Article Snippet: Paragraph title: RNA-sequencing and microarray ... Total RNA was extracted from the retinas of wild-type C57/BL/6J mice at P7 or P14 using the RNeasy Plus Micro Kit (Qiagen), and the RNA quality was confirmed using a 2100 Bioanalyzer (Agilent Technologies).

    Magnetic Beads:

    Article Title: Transcriptomic Profiling of Zebrafish Hair Cells Using RiboTag
    Article Snippet: Remaining supernatant was incubated with 5 μg HA antibody (BioLegend) at 4°C under gentle rotation for 4–6 h. After incubation, supernatants were incubated with 300 μL of rinsed Invitrogen Dynabeads Protein G magnetic beads (Thermo Fisher) overnight, rotating. .. After washing, 350 μL of buffer RLT from the RNeasy Plus Micro kit (Qiagen) was added to the bound beads or reserved input sample and vortexed for 30 s to dissociate bound RNA.

    Isolation:

    Article Title: Follicular regulatory T cells inhibit the development of granzyme B–expressing follicular helper T cells
    Article Snippet: Paragraph title: Cell sorting, RNA isolation, and gene expression analysis. ... Total RNA was collected from freshly sorted cells using the RNeasy Plus Micro kit (QIAGEN). qPCR reactions were performed as previously reported ( ).

    Article Title: miR-219–5p Modulates Cell Growth of Papillary Thyroid Carcinoma by Targeting Estrogen Receptor α
    Article Snippet: .. Total RNA was isolated from tissue samples and cells using the RNeasy Plus Micro Kit (QIAGEN). .. For the detection of mature miRNA, miRNA was reverse transcribed using the TaqMan miRNA Reverse Transcription Kit (Applied Biosystems). qPCR involved the appropriate TaqMan miRNA assay (Applied Biosystems) and a Prism 7500 instrument (Applied Biosystems).

    Article Title: The Telomerase Complex Directly Controls Hematopoietic Stem Cell Differentiation and Senescence in an Induced Pluripotent Stem Cell Model of Telomeropathy
    Article Snippet: .. Gene Expression Assay Genomic DNA and RNA were isolated by column separation using a DNeasy Blood & Tissue Kit (Qiagen 69504) and RNeasy Plus Micro Kit with gDNA elimination (Qiagen 74034), respectively. .. All qPCR assays were run using TaqManprobes and TaqManTM Gene Expression Master Mix (Invitrogen 4369016) in a Roche Lightcycler 480.

    Article Title: Transcriptomic Profiling of Zebrafish Hair Cells Using RiboTag
    Article Snippet: Homogenates were spun at 10,000 g for 10 min at 4°C to remove particulates, and a small sample of clear supernatant was reserved for total RNA isolation (input control, IN). .. After washing, 350 μL of buffer RLT from the RNeasy Plus Micro kit (Qiagen) was added to the bound beads or reserved input sample and vortexed for 30 s to dissociate bound RNA.

    Article Title: Obesity, Diabetes and Energy Homeostasis: Reduced uterine perfusion pressure causes loss of pancreatic β-cell area but normal function in fetal rat offspring
    Article Snippet: .. After an overnight incubation, fetal islets intended for RNA isolation were stored at −80°C in 20 μl RNAlater solution (Invitrogen) and subsequently isolated using the RNeasy Plus Micro Kit (Qiagen), according to the manufacturer’s protocol. .. Isolated (eukaryotic) RNA was quantitated using the Agilent RNA 6000 Nano Kit with an Agilent 2100 Bioanalyzer.

    Labeling:

    Article Title: Effects of Controlled Cortical Impact on the Mouse Brain Vasculome
    Article Snippet: Total RNA was extracted from the fresh preparation of brain microvasculature with the RNeasy Plus Micro kit (Qiagen, Hilden, Germany). .. Microarray hybridization and scanning was done by Expression Analysis Corporation with Affymetrix murine whole genome microarray M430 2.0, with 3-IVT ( in vitro transcription) and Encore Biotin Module for amplification, fragmentation, and labeling.

    Mouse Assay:

    Article Title: Follicular regulatory T cells inhibit the development of granzyme B–expressing follicular helper T cells
    Article Snippet: For isolation of Klrg1+ cells, mice were immunized with SRBC and TFH cells sorted as above but anti-Klrg1 Ab (2F1/KLRG1, BioLegend) was also used for staining. .. Total RNA was collected from freshly sorted cells using the RNeasy Plus Micro kit (QIAGEN). qPCR reactions were performed as previously reported ( ).

    Article Title: Ablation of Kcnj10 expression in retinal explants revealed pivotal roles for Kcnj10 in the proliferation and development of Müller glia
    Article Snippet: .. Total RNA was extracted from the retinas of wild-type C57/BL/6J mice at P7 or P14 using the RNeasy Plus Micro Kit (Qiagen), and the RNA quality was confirmed using a 2100 Bioanalyzer (Agilent Technologies). .. The DNA microarray was performed using cDNA prepared from the retinal explant from E15 (culture days 0, 5, and 14) using the Affymetrix mouse genome 430 2.0 array (2004).

    Polymerase Chain Reaction:

    Article Title: The OV-TL 12/30 Clone of Anti-cytokeratin 7 Antibody as a New Marker of Corneal Conjunctivalization in Patients with Limbal Stem Cell Deficiency
    Article Snippet: Total RNA was extracted from imprints of the central corneal epithelium and upper bulbar conjunctival epithelium of six cadaveric bulbi (RNeasy Plus Micro Kit; Qiagen, Hilden, Germany). .. Reverse transcription reactions (65°C for 5 minutes, 50°C for 50 minutes, and 85°C for 5 minutes), with oligo(dT)20 were followed by 30 PCR cycles (initial denaturation 94°C for 3 minutes, 94°C for 45 seconds, 63°C for 30 seconds, and 72°C for 30 seconds) and a final extension step (10 minutes at 70°C).

    Article Title: Intestinal microbiota sustains inflammation and autoimmunity induced by hypomorphic RAG defects
    Article Snippet: For sorted cells, total RNA was extracted using the RNeasy Plus Micro kit (QIAGEN). .. RNA was retrotranscribed using the High-Capacity cDNA Reverse Transcription kit followed by a preamplification PCR using TaqMan PreAmp Master Mix (Applied Biosystems).

    Article Title: Effects of Stress and Motivation on Performing a Spatial Task
    Article Snippet: Paragraph title: 2.6. Real Time Quantitative Reverse Transcriptase Polymerase Chain Reaction (qRT-PCR) ... Total RNA was extracted from the microdissected brain areas (Qiagen RNeasy Plus Micro Kit; Alameda, CA), cDNA synthesis was conducted (BioRad iScript cDNA; Alameda, CA), and quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to quantify mRNA expression in each region of the brain.

    Article Title: Stellate Cells, Hepatocytes, and Endothelial Cells Imprint the Kupffer Cell Identity on Monocytes Colonizing the Liver Macrophage Niche
    Article Snippet: Gene expression analysis by real-time RT-PCR RNA was purified from 2,5x104 sorted cells using a RNeasy Plus micro kit (QIAGEN). .. Gene expression was assayed by real-time RT-PCR using a SensiFast SYBR No-Rox kit (Bioline) on a PCR amplification and detection instrument (LightCycler 480; Roche) with the primers listed in the .

    Article Title: Obesity, Diabetes and Energy Homeostasis: Reduced uterine perfusion pressure causes loss of pancreatic β-cell area but normal function in fetal rat offspring
    Article Snippet: After an overnight incubation, fetal islets intended for RNA isolation were stored at −80°C in 20 μl RNAlater solution (Invitrogen) and subsequently isolated using the RNeasy Plus Micro Kit (Qiagen), according to the manufacturer’s protocol. .. Amplification was carried out using Power SYBR Green PCR Master Mix in QuantStudio Flex Real-Time PCR System for 40 cycles (Applied Biosystems).

    Article Title: Glutamine Stimulates Biosynthesis and Secretion of Insulin-like Growth Factor 2 (IGF2), an Autocrine Regulator of Beta Cell Mass and Function *
    Article Snippet: Total RNA was extracted using RNeasy Plus Micro Kit from Qiagen, and cDNAs were synthesized using SuperScript II RNase H reverse transcriptase (Invitrogen), and 50 pmol of random hexamers (Applied Biosystems, Basel, Switzerland). .. Amplification was performed in a 20-μl reaction mixture including 1× QuantiTectTM SYBR Green PCR Master Mix (Qiagen) and 10 pmol of each primer.

    Construct:

    Article Title: CD38 Inhibits Prostate Cancer Metabolism and Proliferation by Reducing Cellular NAD+ Pools
    Article Snippet: LNCaP cells transfected with either an empty vector or CD38 expression construct were plated (5 × 105 cells/dish) in 10cm dishes and treated with doxycycline (1μg/ml) for 96Hrs (N = 5 per group). .. Total RNA was purified from cell lines using the RNeasy Plus Micro Kit (Qiagen, Germantown, MD, USA) with genomic DNA removal.

    Staining:

    Article Title: Follicular regulatory T cells inhibit the development of granzyme B–expressing follicular helper T cells
    Article Snippet: For isolation of Klrg1+ cells, mice were immunized with SRBC and TFH cells sorted as above but anti-Klrg1 Ab (2F1/KLRG1, BioLegend) was also used for staining. .. Total RNA was collected from freshly sorted cells using the RNeasy Plus Micro kit (QIAGEN). qPCR reactions were performed as previously reported ( ).

    Article Title: Expression profiles of stress-related genes in islets from donors with progressively impaired glucose metabolism
    Article Snippet: The Islets of Langerhans were localized through islet auto-fluorescence and their localization was confirmed using the corresponding object glasses stained for CD45 and insulin in order to avoid islets with an ongoing intra-insular infiltration. .. RNA extraction was performed using a Qiagen RNeasy Plus Micro kit (Qiagen) according to the manufacturer's instructions.

    Purification:

    Article Title: Stellate Cells, Hepatocytes, and Endothelial Cells Imprint the Kupffer Cell Identity on Monocytes Colonizing the Liver Macrophage Niche
    Article Snippet: .. Gene expression analysis by real-time RT-PCR RNA was purified from 2,5x104 sorted cells using a RNeasy Plus micro kit (QIAGEN). .. RNA was reverse transcribed to cDNA with a Sensifast cDNA synthesis kit (Bioline).

    Article Title: CD38 Inhibits Prostate Cancer Metabolism and Proliferation by Reducing Cellular NAD+ Pools
    Article Snippet: .. Total RNA was purified from cell lines using the RNeasy Plus Micro Kit (Qiagen, Germantown, MD, USA) with genomic DNA removal. .. RNA integrity (RIN) was assessed via electrophoretic tracing on an Agilent Bioanalyzer.

    Plasmid Preparation:

    Article Title: CD38 Inhibits Prostate Cancer Metabolism and Proliferation by Reducing Cellular NAD+ Pools
    Article Snippet: LNCaP cells transfected with either an empty vector or CD38 expression construct were plated (5 × 105 cells/dish) in 10cm dishes and treated with doxycycline (1μg/ml) for 96Hrs (N = 5 per group). .. Total RNA was purified from cell lines using the RNeasy Plus Micro Kit (Qiagen, Germantown, MD, USA) with genomic DNA removal.

    Real-time Polymerase Chain Reaction:

    Article Title: Follicular regulatory T cells inhibit the development of granzyme B–expressing follicular helper T cells
    Article Snippet: .. Total RNA was collected from freshly sorted cells using the RNeasy Plus Micro kit (QIAGEN). qPCR reactions were performed as previously reported ( ). ..

    Article Title: The Transferrin Receptor CD71 Delineates Functionally Distinct Airway Macrophage Subsets during Idiopathic Pulmonary Fibrosis
    Article Snippet: .. Real-Time PCR RNA extraction was performed using the RNeasy Plus Micro Kit (Qiagen) according to the manufacturer’s protocol. .. Total RNA was reverse transcribed into cDNA using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific) as per the manufacturer’s instructions.

    Article Title: miR-219–5p Modulates Cell Growth of Papillary Thyroid Carcinoma by Targeting Estrogen Receptor α
    Article Snippet: Paragraph title: RNA extraction and quantitative PCR (qPCR) ... Total RNA was isolated from tissue samples and cells using the RNeasy Plus Micro Kit (QIAGEN).

    Article Title: The Telomerase Complex Directly Controls Hematopoietic Stem Cell Differentiation and Senescence in an Induced Pluripotent Stem Cell Model of Telomeropathy
    Article Snippet: Gene Expression Assay Genomic DNA and RNA were isolated by column separation using a DNeasy Blood & Tissue Kit (Qiagen 69504) and RNeasy Plus Micro Kit with gDNA elimination (Qiagen 74034), respectively. .. All qPCR assays were run using TaqManprobes and TaqManTM Gene Expression Master Mix (Invitrogen 4369016) in a Roche Lightcycler 480.

    Article Title: Obesity, Diabetes and Energy Homeostasis: Reduced uterine perfusion pressure causes loss of pancreatic β-cell area but normal function in fetal rat offspring
    Article Snippet: Paragraph title: RNA isolation and quantitative PCR. ... After an overnight incubation, fetal islets intended for RNA isolation were stored at −80°C in 20 μl RNAlater solution (Invitrogen) and subsequently isolated using the RNeasy Plus Micro Kit (Qiagen), according to the manufacturer’s protocol.

    Article Title: Glutamine Stimulates Biosynthesis and Secretion of Insulin-like Growth Factor 2 (IGF2), an Autocrine Regulator of Beta Cell Mass and Function *
    Article Snippet: Paragraph title: Quantitative PCR Analysis ... Total RNA was extracted using RNeasy Plus Micro Kit from Qiagen, and cDNAs were synthesized using SuperScript II RNase H reverse transcriptase (Invitrogen), and 50 pmol of random hexamers (Applied Biosystems, Basel, Switzerland).

    RNA Extraction:

    Article Title: The Transferrin Receptor CD71 Delineates Functionally Distinct Airway Macrophage Subsets during Idiopathic Pulmonary Fibrosis
    Article Snippet: .. Real-Time PCR RNA extraction was performed using the RNeasy Plus Micro Kit (Qiagen) according to the manufacturer’s protocol. .. Total RNA was reverse transcribed into cDNA using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific) as per the manufacturer’s instructions.

    Article Title: miR-219–5p Modulates Cell Growth of Papillary Thyroid Carcinoma by Targeting Estrogen Receptor α
    Article Snippet: Paragraph title: RNA extraction and quantitative PCR (qPCR) ... Total RNA was isolated from tissue samples and cells using the RNeasy Plus Micro Kit (QIAGEN).

    Article Title: Expression profiles of stress-related genes in islets from donors with progressively impaired glucose metabolism
    Article Snippet: .. RNA extraction was performed using a Qiagen RNeasy Plus Micro kit (Qiagen) according to the manufacturer's instructions. .. qPCR analysis cDNA synthesis from the RNA extracted from the islet tissue and subsequent pre-amplification and expression analysis were performed using kits from Qiagen according to the manufacturer's instructions as described previously.

    In Vitro:

    Article Title: Effects of Controlled Cortical Impact on the Mouse Brain Vasculome
    Article Snippet: Total RNA was extracted from the fresh preparation of brain microvasculature with the RNeasy Plus Micro kit (Qiagen, Hilden, Germany). .. Microarray hybridization and scanning was done by Expression Analysis Corporation with Affymetrix murine whole genome microarray M430 2.0, with 3-IVT ( in vitro transcription) and Encore Biotin Module for amplification, fragmentation, and labeling.

    Homogenization:

    Article Title: Transcriptomic Profiling of Zebrafish Hair Cells Using RiboTag
    Article Snippet: Larvae were resuspended and homogenized in 1 mL of supplemented homogenization buffer (50 mM Tris-HCl pH.7, 100 mM KCl, 12 mM MgCl2 , 1% Nonidet P-40, 1 mM 1,4-Dithiothreitol, 1X protease inhibitor cocktail, 200 U/mL RNAsin, 100 μg/mL cycloheximide, 1 mg/mL heparin) by douncing on ice. .. After washing, 350 μL of buffer RLT from the RNeasy Plus Micro kit (Qiagen) was added to the bound beads or reserved input sample and vortexed for 30 s to dissociate bound RNA.

    Laser Capture Microdissection:

    Article Title: Expression profiles of stress-related genes in islets from donors with progressively impaired glucose metabolism
    Article Snippet: The islets were captured on Arcturus CapSure HS LCM Caps (Thermo Fisher Scientific) and the caps were subsequently incubated for 30 min at 42°C in 20 μl RLT Buffer Plus (Qiagen, Hilden, Germany) containing 1% beta-mercaptoethanol. .. RNA extraction was performed using a Qiagen RNeasy Plus Micro kit (Qiagen) according to the manufacturer's instructions.

    Incubation:

    Article Title: Expression profiles of stress-related genes in islets from donors with progressively impaired glucose metabolism
    Article Snippet: The islets were captured on Arcturus CapSure HS LCM Caps (Thermo Fisher Scientific) and the caps were subsequently incubated for 30 min at 42°C in 20 μl RLT Buffer Plus (Qiagen, Hilden, Germany) containing 1% beta-mercaptoethanol. .. RNA extraction was performed using a Qiagen RNeasy Plus Micro kit (Qiagen) according to the manufacturer's instructions.

    Article Title: Transcriptomic Profiling of Zebrafish Hair Cells Using RiboTag
    Article Snippet: Following incubation, bound beads were rinsed three times with 800 μL high salt buffer (50 mM Tris-HCl pH.7, 300 mM KCl, 12 mM MgCl2 , 1% Nonidet P-40, 1 mM 1,4-Dithiothreitol, 100 μg/mL cycloheximide) at 4°C for 10 min, rotating. .. After washing, 350 μL of buffer RLT from the RNeasy Plus Micro kit (Qiagen) was added to the bound beads or reserved input sample and vortexed for 30 s to dissociate bound RNA.

    Article Title: Obesity, Diabetes and Energy Homeostasis: Reduced uterine perfusion pressure causes loss of pancreatic β-cell area but normal function in fetal rat offspring
    Article Snippet: .. After an overnight incubation, fetal islets intended for RNA isolation were stored at −80°C in 20 μl RNAlater solution (Invitrogen) and subsequently isolated using the RNeasy Plus Micro Kit (Qiagen), according to the manufacturer’s protocol. .. Isolated (eukaryotic) RNA was quantitated using the Agilent RNA 6000 Nano Kit with an Agilent 2100 Bioanalyzer.

    Immunoprecipitation:

    Article Title: Transcriptomic Profiling of Zebrafish Hair Cells Using RiboTag
    Article Snippet: Paragraph title: Tg(myo6b:RiboTag) translatome immunoprecipitation ... After washing, 350 μL of buffer RLT from the RNeasy Plus Micro kit (Qiagen) was added to the bound beads or reserved input sample and vortexed for 30 s to dissociate bound RNA.

    FACS:

    Article Title: Follicular regulatory T cells inhibit the development of granzyme B–expressing follicular helper T cells
    Article Snippet: Paragraph title: Cell sorting, RNA isolation, and gene expression analysis. ... Total RNA was collected from freshly sorted cells using the RNeasy Plus Micro kit (QIAGEN). qPCR reactions were performed as previously reported ( ).

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    Qiagen rneasy plus micro kit
    Rneasy Plus Micro Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 426 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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