Structured Review

Thermo Fisher 72o c
72o C, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/72o c/product/Thermo Fisher
Average 91 stars, based on 33 article reviews
Price from $9.99 to $1999.99
72o c - by Bioz Stars, 2020-09
91/100 stars

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Related Articles

Clone Assay:

Article Title: PCR-Activated Cell Sorting for Cultivation-Free Enrichment and Sequencing of Rare Microbes
Article Snippet: .. The mixture is then thermocycled with the following conditions: 10 min at 95o C, 35 cycles of 10 s at 95o C, 15 s at 55o C and 30 s at 72o C. The PCR product is then cloned into a pCR4-TOPO vector (Life Technologies) using a TOPO TA cloning kit for sequencing (Life Technologies), as following the manufacturer’s instructions. .. This is transformed into electrocompetent E. coli TOP10 bacteria and streaked onto LB plates with 50μgml-1 kanamycin for growth at 37o C overnight.

Amplification:

Article Title: The prevalence of Y chromosome microdeletions in Pakistani infertile men
Article Snippet: .. Amplification cycles were proceeded by initial denaturation for 5 min at 95o C and final extension at 72o C for 5 min. All reagents were purchased from Fermentas (USA) and primers were made from Operon, Germany. ..

Agarose Gel Electrophoresis:

Article Title: INSR gene variation is associated with decreased insulin sensitivity in Iraqi women with PCOs
Article Snippet: .. Cycling parameters were denaturated at 94o C for 5 minutes, 35 cycles with 94o C for 45 seconds, 55o C for 40 seconds, 72o C for 60 seconds, and 72o C for 10 min. PCR products (317-bp) digested with PmlI (Thermo Scientific, USA) for 16 hours at 37o C. Digested DNA fragments were electrophoresed on a 2% agarose gel containing ethidium bromide and visualized by UV trans-illuminator spectroline (USA). ..

Article Title: H1299R in coagulation Factor V and Glu429Ala in MTHFR genes in recurrent pregnancy loss in Sari, Mazandaran
Article Snippet: .. PCR conditions included one step initial denaturation (94o C for 3 min), 35 cycles (94o C for 45 sec, 63.6o C for 40 sec and 72o C for 40 sec) and a final extension at 72o C for 5 min. PCR products were then electrophoresed in a 1.5% agarose gel (Fermentas, Germany). .. Amplified PCR products were subjected to enzymatic digestion with RsaI restriction enzyme (Fermentas, Germany) for 16 hr at 37o C and visualized after separation by 3% agarose gel electrophoresis followed by staining with ethidium bromide ( ).

Article Title: Significant correlation of angiotensin converting enzyme and glycoprotein IIIa genes polymorphisms with unexplained recurrent pregnancy loss in north of Iran
Article Snippet: .. PCR conditions for ACE genotyping included one step initial denaturation (94o C for 3 min), 35 cycles (94o C for 45 sec, 60.5o C for 50 sec, and 72o C for 40 sec) and a final extension at 72o C for 5 min. PCR conditions for GPIIIa genotyping also included one step initial denaturation (94o C for 3 min), 35 cycles (94o C for 35 sec, 58o C for 40 sec, and 72o C for 40 sec) and a final extension at 72o C for 5 min. Then, PCR products were electrophoresed in a 1.5% agarose gel (Fermentas, Sankt Leon-Rot, Germany). ..

Article Title: Mutation analysis of exon1 of bone morphogenetic protein-15 gene in Iranian patients with polycystic ovarian syndrome
Article Snippet: .. The initial step for denaturation was 5 minutes of 95o C and the final segment of the PCR (final extension) was 5 min of 72o C. The resulted PCR products were tested on agarose gel ( ) for ideal concentration and sequenced by an automated sequencer (ABI 3730; Applied Biosystems) in order to perform mutation analysis ( ). ..

Polymerase Chain Reaction:

Article Title: INSR gene variation is associated with decreased insulin sensitivity in Iraqi women with PCOs
Article Snippet: .. Cycling parameters were denaturated at 94o C for 5 minutes, 35 cycles with 94o C for 45 seconds, 55o C for 40 seconds, 72o C for 60 seconds, and 72o C for 10 min. PCR products (317-bp) digested with PmlI (Thermo Scientific, USA) for 16 hours at 37o C. Digested DNA fragments were electrophoresed on a 2% agarose gel containing ethidium bromide and visualized by UV trans-illuminator spectroline (USA). ..

Article Title: H1299R in coagulation Factor V and Glu429Ala in MTHFR genes in recurrent pregnancy loss in Sari, Mazandaran
Article Snippet: .. PCR conditions included one step initial denaturation (94o C for 3 min), 35 cycles (94o C for 45 sec, 63.6o C for 40 sec and 72o C for 40 sec) and a final extension at 72o C for 5 min. PCR products were then electrophoresed in a 1.5% agarose gel (Fermentas, Germany). .. Amplified PCR products were subjected to enzymatic digestion with RsaI restriction enzyme (Fermentas, Germany) for 16 hr at 37o C and visualized after separation by 3% agarose gel electrophoresis followed by staining with ethidium bromide ( ).

Article Title: Significant correlation of angiotensin converting enzyme and glycoprotein IIIa genes polymorphisms with unexplained recurrent pregnancy loss in north of Iran
Article Snippet: .. PCR conditions for ACE genotyping included one step initial denaturation (94o C for 3 min), 35 cycles (94o C for 45 sec, 60.5o C for 50 sec, and 72o C for 40 sec) and a final extension at 72o C for 5 min. PCR conditions for GPIIIa genotyping also included one step initial denaturation (94o C for 3 min), 35 cycles (94o C for 35 sec, 58o C for 40 sec, and 72o C for 40 sec) and a final extension at 72o C for 5 min. Then, PCR products were electrophoresed in a 1.5% agarose gel (Fermentas, Sankt Leon-Rot, Germany). ..

Article Title: Mutation analysis of exon1 of bone morphogenetic protein-15 gene in Iranian patients with polycystic ovarian syndrome
Article Snippet: .. The initial step for denaturation was 5 minutes of 95o C and the final segment of the PCR (final extension) was 5 min of 72o C. The resulted PCR products were tested on agarose gel ( ) for ideal concentration and sequenced by an automated sequencer (ABI 3730; Applied Biosystems) in order to perform mutation analysis ( ). ..

Article Title: Whole-Exome Sequencing in the Differential Diagnosis of Primary Adrenal Insufficiency in Children
Article Snippet: .. This was then followed by 25 cycles of 95o C for 30 s, 55o C for 30 s and 72o C for 1 min. After the last cycle, there is an extension step at 72o C for 5 min. PCR products were sequenced using ABI Prism Big Dye Sequencing kit and an ABI 377 automated DNA sequencer (Applied Biosystems) as described in the manufacturer’s instructions. ..

Article Title: Development of a Sensitive and Specific Enzyme-Linked Immunosorbent Assay for Detecting and Quantifying CMY-2 and SHV ?-Lactamases
Article Snippet: .. This step was followed by a final extension at 72o C for 10 min. PCR products were run on 1% agarose gels, stained with ethidium bromide, and photographed with UV illumination. φX174 replicative-form DNA Hae III fragments (Gibco BRL Life Technologies) were used to assess PCR product sizes. ..

Article Title: PCR-Activated Cell Sorting for Cultivation-Free Enrichment and Sequencing of Rare Microbes
Article Snippet: .. The mixture is then thermocycled with the following conditions: 10 min at 95o C, 35 cycles of 10 s at 95o C, 15 s at 55o C and 30 s at 72o C. The PCR product is then cloned into a pCR4-TOPO vector (Life Technologies) using a TOPO TA cloning kit for sequencing (Life Technologies), as following the manufacturer’s instructions. .. This is transformed into electrocompetent E. coli TOP10 bacteria and streaked onto LB plates with 50μgml-1 kanamycin for growth at 37o C overnight.

Mutagenesis:

Article Title: Mutation analysis of exon1 of bone morphogenetic protein-15 gene in Iranian patients with polycystic ovarian syndrome
Article Snippet: .. The initial step for denaturation was 5 minutes of 95o C and the final segment of the PCR (final extension) was 5 min of 72o C. The resulted PCR products were tested on agarose gel ( ) for ideal concentration and sequenced by an automated sequencer (ABI 3730; Applied Biosystems) in order to perform mutation analysis ( ). ..

Size-exclusion Chromatography:

Article Title: H1299R in coagulation Factor V and Glu429Ala in MTHFR genes in recurrent pregnancy loss in Sari, Mazandaran
Article Snippet: .. PCR conditions included one step initial denaturation (94o C for 3 min), 35 cycles (94o C for 45 sec, 63.6o C for 40 sec and 72o C for 40 sec) and a final extension at 72o C for 5 min. PCR products were then electrophoresed in a 1.5% agarose gel (Fermentas, Germany). .. Amplified PCR products were subjected to enzymatic digestion with RsaI restriction enzyme (Fermentas, Germany) for 16 hr at 37o C and visualized after separation by 3% agarose gel electrophoresis followed by staining with ethidium bromide ( ).

Article Title: Significant correlation of angiotensin converting enzyme and glycoprotein IIIa genes polymorphisms with unexplained recurrent pregnancy loss in north of Iran
Article Snippet: .. PCR conditions for ACE genotyping included one step initial denaturation (94o C for 3 min), 35 cycles (94o C for 45 sec, 60.5o C for 50 sec, and 72o C for 40 sec) and a final extension at 72o C for 5 min. PCR conditions for GPIIIa genotyping also included one step initial denaturation (94o C for 3 min), 35 cycles (94o C for 35 sec, 58o C for 40 sec, and 72o C for 40 sec) and a final extension at 72o C for 5 min. Then, PCR products were electrophoresed in a 1.5% agarose gel (Fermentas, Sankt Leon-Rot, Germany). ..

TA Cloning:

Article Title: PCR-Activated Cell Sorting for Cultivation-Free Enrichment and Sequencing of Rare Microbes
Article Snippet: .. The mixture is then thermocycled with the following conditions: 10 min at 95o C, 35 cycles of 10 s at 95o C, 15 s at 55o C and 30 s at 72o C. The PCR product is then cloned into a pCR4-TOPO vector (Life Technologies) using a TOPO TA cloning kit for sequencing (Life Technologies), as following the manufacturer’s instructions. .. This is transformed into electrocompetent E. coli TOP10 bacteria and streaked onto LB plates with 50μgml-1 kanamycin for growth at 37o C overnight.

Concentration Assay:

Article Title: Mutation analysis of exon1 of bone morphogenetic protein-15 gene in Iranian patients with polycystic ovarian syndrome
Article Snippet: .. The initial step for denaturation was 5 minutes of 95o C and the final segment of the PCR (final extension) was 5 min of 72o C. The resulted PCR products were tested on agarose gel ( ) for ideal concentration and sequenced by an automated sequencer (ABI 3730; Applied Biosystems) in order to perform mutation analysis ( ). ..

Sequencing:

Article Title: Whole-Exome Sequencing in the Differential Diagnosis of Primary Adrenal Insufficiency in Children
Article Snippet: .. This was then followed by 25 cycles of 95o C for 30 s, 55o C for 30 s and 72o C for 1 min. After the last cycle, there is an extension step at 72o C for 5 min. PCR products were sequenced using ABI Prism Big Dye Sequencing kit and an ABI 377 automated DNA sequencer (Applied Biosystems) as described in the manufacturer’s instructions. ..

Article Title: PCR-Activated Cell Sorting for Cultivation-Free Enrichment and Sequencing of Rare Microbes
Article Snippet: .. The mixture is then thermocycled with the following conditions: 10 min at 95o C, 35 cycles of 10 s at 95o C, 15 s at 55o C and 30 s at 72o C. The PCR product is then cloned into a pCR4-TOPO vector (Life Technologies) using a TOPO TA cloning kit for sequencing (Life Technologies), as following the manufacturer’s instructions. .. This is transformed into electrocompetent E. coli TOP10 bacteria and streaked onto LB plates with 50μgml-1 kanamycin for growth at 37o C overnight.

Staining:

Article Title: Development of a Sensitive and Specific Enzyme-Linked Immunosorbent Assay for Detecting and Quantifying CMY-2 and SHV ?-Lactamases
Article Snippet: .. This step was followed by a final extension at 72o C for 10 min. PCR products were run on 1% agarose gels, stained with ethidium bromide, and photographed with UV illumination. φX174 replicative-form DNA Hae III fragments (Gibco BRL Life Technologies) were used to assess PCR product sizes. ..

Plasmid Preparation:

Article Title: PCR-Activated Cell Sorting for Cultivation-Free Enrichment and Sequencing of Rare Microbes
Article Snippet: .. The mixture is then thermocycled with the following conditions: 10 min at 95o C, 35 cycles of 10 s at 95o C, 15 s at 55o C and 30 s at 72o C. The PCR product is then cloned into a pCR4-TOPO vector (Life Technologies) using a TOPO TA cloning kit for sequencing (Life Technologies), as following the manufacturer’s instructions. .. This is transformed into electrocompetent E. coli TOP10 bacteria and streaked onto LB plates with 50μgml-1 kanamycin for growth at 37o C overnight.

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    Thermo Fisher 72o c
    72o C, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/72o c/product/Thermo Fisher
    Average 91 stars, based on 33 article reviews
    Price from $9.99 to $1999.99
    72o c - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

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