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The pDNAs indicate probe DNAs that are complementary to the respective target miRNAs. <t>IRDye800</t> is an acceptor. a TG-LRET using NIR long-lived UCNP as a donor exhibits higher sensitivity than ( b ) SS-LRET using NIR short-lived UCNP as a donor. UCNP, upconversion nanoparticle; LRET, luminescence resonance energy transfer; EM, energy migration; ET, energy transfer. In a , b dark blue and light blue spheres represent Yb 3+ ions in the excited and ground states, respectively. Part of the figure was created in BioRender. https://BioRender.com/7185k6m .
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The pDNAs indicate probe DNAs that are complementary to the respective target miRNAs. IRDye800 is an acceptor. a TG-LRET using NIR long-lived UCNP as a donor exhibits higher sensitivity than ( b ) SS-LRET using NIR short-lived UCNP as a donor. UCNP, upconversion nanoparticle; LRET, luminescence resonance energy transfer; EM, energy migration; ET, energy transfer. In a , b dark blue and light blue spheres represent Yb 3+ ions in the excited and ground states, respectively. Part of the figure was created in BioRender. https://BioRender.com/7185k6m .

Journal: Nature Communications

Article Title: Near-infrared long lifetime upconversion nanoparticles for ultrasensitive microRNA detection via time-gated luminescence resonance energy transfer

doi: 10.1038/s41467-025-62802-x

Figure Lengend Snippet: The pDNAs indicate probe DNAs that are complementary to the respective target miRNAs. IRDye800 is an acceptor. a TG-LRET using NIR long-lived UCNP as a donor exhibits higher sensitivity than ( b ) SS-LRET using NIR short-lived UCNP as a donor. UCNP, upconversion nanoparticle; LRET, luminescence resonance energy transfer; EM, energy migration; ET, energy transfer. In a , b dark blue and light blue spheres represent Yb 3+ ions in the excited and ground states, respectively. Part of the figure was created in BioRender. https://BioRender.com/7185k6m .

Article Snippet: Amine-modified donor (50 μg) was mixed with IRDye800 NHS ester (LI-COR Biosciences, Lincoln, NE, USA) at various concentrations (0, 10 −0.5 , 10 0 , 10 0.5 , 10 1 μM) in HEPES buffer (10 mM, pH 7.4).

Techniques: Förster Resonance Energy Transfer, Migration

a Schematic diagram illustrating the energy transfer of L-donor compared to S-donor, both with acceptor (IRDye800) bound to the surface. UCL decay curves of the ( b ) L-donor, ( c ) M-donor, and ( d ) S-donor as a function of acceptor concentration under a 980 nm short pulse (5 ns) excitation. UCL decay curves of the ( e ) L-donor, ( f ) M-donor, and ( g ) S-donor as a function of acceptor concentration under a 980 nm long pulse (5 ms). The changes in luminescence decay time values (-Δτ D ) were compared for ( h ) the L-donor samples in ( b ) and ( e ), for ( i ) the M-donor samples in ( c ) and ( f ), and for ( j ) the S-donor samples in ( d ) and ( g ). L-donor, NIR long-lived luminescent UCNP donor; M-donor, NIR intermediate-lived luminescent UCNP donor; S-donor, NIR short-lived luminescent UCNP donor; TG-LRET, time-gated LRET; SS-LRET, LRET under steady-state excitation. Source data are provided as a file.

Journal: Nature Communications

Article Title: Near-infrared long lifetime upconversion nanoparticles for ultrasensitive microRNA detection via time-gated luminescence resonance energy transfer

doi: 10.1038/s41467-025-62802-x

Figure Lengend Snippet: a Schematic diagram illustrating the energy transfer of L-donor compared to S-donor, both with acceptor (IRDye800) bound to the surface. UCL decay curves of the ( b ) L-donor, ( c ) M-donor, and ( d ) S-donor as a function of acceptor concentration under a 980 nm short pulse (5 ns) excitation. UCL decay curves of the ( e ) L-donor, ( f ) M-donor, and ( g ) S-donor as a function of acceptor concentration under a 980 nm long pulse (5 ms). The changes in luminescence decay time values (-Δτ D ) were compared for ( h ) the L-donor samples in ( b ) and ( e ), for ( i ) the M-donor samples in ( c ) and ( f ), and for ( j ) the S-donor samples in ( d ) and ( g ). L-donor, NIR long-lived luminescent UCNP donor; M-donor, NIR intermediate-lived luminescent UCNP donor; S-donor, NIR short-lived luminescent UCNP donor; TG-LRET, time-gated LRET; SS-LRET, LRET under steady-state excitation. Source data are provided as a file.

Article Snippet: Amine-modified donor (50 μg) was mixed with IRDye800 NHS ester (LI-COR Biosciences, Lincoln, NE, USA) at various concentrations (0, 10 −0.5 , 10 0 , 10 0.5 , 10 1 μM) in HEPES buffer (10 mM, pH 7.4).

Techniques: Concentration Assay