cat no 69504  (Qiagen)


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    Qiagen cat no 69504
    Cat No 69504, supplied by Qiagen, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cat no 69504/product/Qiagen
    Average 77 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    cat no 69504 - by Bioz Stars, 2020-01
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    Related Articles

    Methylation Sequencing:

    Article Title: Dynamic Imprinting of the Treg Cell-Specific Epigenetic Signature in Developing Thymic Regulatory T Cells
    Article Snippet: DNA Methylation Analysis Genomic DNA was isolated from sorted CD4SP thymocyte subsets using the DNeasy® Blood & Tissue Kit (Qiagen) and concentrated using the DNA Clean & Concentrator Kit (Zymo Research), both following the manufacturers' protocols. .. Methylation analysis of the TSDR and other Treg cell-specific epigenetic signature genes was performed using bisulfite sequencing as described before ( ).

    Clone Assay:

    Article Title: A human expression system based on HEK293 for the stable production of recombinant erythropoietin
    Article Snippet: Sequencing of targeted GLUL genomic locus Genomic DNA was isolated from potential GLUL -KO cell lines using DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany). .. Cycling conditions were programmed as follows: 95 °C for 2 min, followed by 35 cycles of 95 °C for 20 s, 55 °C for 10 s, and 70 °C for 15 s. Further extension was carried out with Taq polymerase (Thermo Fisher Scientific) to add 3′ adenine overhangs for cloning into a T-vector containing complementary thymidine residues.

    Amplification:

    Article Title: HIF-1α protects against oxidative stress by directly targeting mitochondria
    Article Snippet: .. 2.6 Mitochondrial DNA amplification Total cellular DNA was isolated using DNeasy Blood & Tissue Kit (Qiagen, Dusseldorf, Germany) and quantified using a NanoDrop2000 spectrophotometer (Bio-Tek). .. Primers were designed using NCBI Primer-BLAST and synthesized by Life Technologies (Shanghai, China).

    Article Title: Immunodominant, protective response to the parasite Toxoplasma gondii requires antigen processing in the endoplasmic reticulum
    Article Snippet: Genomic DNA was extracted from less than 10 mg ground tissue with the DNeasy Blood & Tissue kit (Qiagen). .. A fragment of the T. gondii B1 gene was amplified by PCR along with increasing amounts of a competitive internal standard DNA added in the same reaction.

    Article Title: Diversity, Activity, and Evolution of CRISPR Loci in Streptococcus thermophilus ▿ ▿ †
    Article Snippet: S. thermophilus genomic DNA was extracted and purified by using a DNeasy Blood & Tissue kit (Qiagen). .. Prior to sequencing, each CRISPR locus was amplified by PCR with LA-Taq DNA polymerase (Takara/Lonza).

    Synthesized:

    Article Title: HIF-1α protects against oxidative stress by directly targeting mitochondria
    Article Snippet: 2.6 Mitochondrial DNA amplification Total cellular DNA was isolated using DNeasy Blood & Tissue Kit (Qiagen, Dusseldorf, Germany) and quantified using a NanoDrop2000 spectrophotometer (Bio-Tek). .. Primers were designed using NCBI Primer-BLAST and synthesized by Life Technologies (Shanghai, China).

    Article Title: Expression quantitative trait loci detected in cell lines are often present in primary tissues
    Article Snippet: We extracted DNA from each tissue using the Qiagen DNeasy Blood & Tissue Kit (Qiagen, Germantown, MD). .. RNA was extracted from the tissues using Trizol (Invitrogen, Carisbad, CA) and first strand cDNA was synthesized using a poly-T oligo and the superscript kit (Invitrogen, Carisbad, CA).

    Cytometry:

    Article Title: Immunodominant, protective response to the parasite Toxoplasma gondii requires antigen processing in the endoplasmic reticulum
    Article Snippet: The number of infected splenocytes and peritoneal macrophages was determined by measurement of the percentage of GFP+ cells by flow cytometry. .. Genomic DNA was extracted from less than 10 mg ground tissue with the DNeasy Blood & Tissue kit (Qiagen).

    Quantitative RT-PCR:

    Article Title: HIF-1α protects against oxidative stress by directly targeting mitochondria
    Article Snippet: 2.6 Mitochondrial DNA amplification Total cellular DNA was isolated using DNeasy Blood & Tissue Kit (Qiagen, Dusseldorf, Germany) and quantified using a NanoDrop2000 spectrophotometer (Bio-Tek). .. Sequences are listed in . qRT-PCR was performed using SsoFast EvaGreen Supermix (Bio-Rad) and a CFX96 Real-time PCR Detection System (Bio-Rad).

    Real-time Polymerase Chain Reaction:

    Article Title: Highly sensitive detection of sentinel lymph node metastasis of breast cancer by digital PCR for RASSF1A methylation
    Article Snippet: DNA and mRNA were extracted from 10E+6 cells from each cell line using the DNeasy Blood & Tissue Kit and the RNeasy Mini Kit (both Qiagen GmbH), respectively. .. Briefly, 1 µg total RNA was reverse-transcribed into cDNA using the ReverTra Ace® qPCR RT kit (Toyobo Life Science), and CK19 mRNA expression was assessed using the Light Cycler 480 Real-time PCR System (Roche Applied Science) with the following conditions: 95°C for 10 min, followed by 50 cycles at 95°C for 15 sec and 60°C for 60 sec, with a final cycle at 50°C for 10 sec. KRT19 (CK19) TaqMan® Gene Expression Assays (Hs01051611_gH; Applied Biosystems; Thermo Fisher Scientific, Inc.) were used to conduct real-time PCR.

    Article Title: Efficacy of three innovative bacterin vaccines against experimental infection with Mycoplasma hyopneumoniae
    Article Snippet: .. Deoxyribonucleic acid was extracted from the BAL fluid using a commercial kit (DNeasy® Blood & Tissue kit, Qiagen, Venlo, The Netherlands) and a quantitative PCR (qPCR) was performed according to Marois et al. [ ] to measure the number of M. hyopneumoniae organisms. ..

    Article Title: HIF-1α protects against oxidative stress by directly targeting mitochondria
    Article Snippet: 2.6 Mitochondrial DNA amplification Total cellular DNA was isolated using DNeasy Blood & Tissue Kit (Qiagen, Dusseldorf, Germany) and quantified using a NanoDrop2000 spectrophotometer (Bio-Tek). .. Sequences are listed in . qRT-PCR was performed using SsoFast EvaGreen Supermix (Bio-Rad) and a CFX96 Real-time PCR Detection System (Bio-Rad).

    Incubation:

    Article Title: Application of different DNA extraction procedures, library preparation protocols and sequencing platforms: impact on sequencing results
    Article Snippet: At the end of the incubation period, 500 μl of broth were centrifuged at 5000 x g for 10’. .. Pellets obtained from cultures BACT1 to BACT4 were suspended in 180 μl of ATL buffer and submitted to DNA extraction using the DNeasy Blood & Tissue Kit (Qiagen, Milan, Italy).

    Article Title: What drives phenotypic divergence among coral clonemates of Acropora palmata?. What drives phenotypic divergence among coral clonemates of Acropora palmata?
    Article Snippet: 2.4 Methylrad library preparation and bioinformatics Coral tissue samples were snap frozen in liquid nitrogen and kept at −80°C before extraction using the DNeasy Blood & Tissue Kit (Qiagen) with the following modifications. .. Time of incubation in extraction buffer was increased to 16–20 hr.

    Article Title: Efficacy of three innovative bacterin vaccines against experimental infection with Mycoplasma hyopneumoniae
    Article Snippet: Deoxyribonucleic acid was extracted from the BAL fluid using a commercial kit (DNeasy® Blood & Tissue kit, Qiagen, Venlo, The Netherlands) and a quantitative PCR (qPCR) was performed according to Marois et al. [ ] to measure the number of M. hyopneumoniae organisms. .. The agar plates were incubated in a 5% CO2 -enriched atmosphere at 35 ± 2 °C for 48 h to detect the presence of other respiratory bacteria.

    Infection:

    Article Title: Efficacy of three innovative bacterin vaccines against experimental infection with Mycoplasma hyopneumoniae
    Article Snippet: Quantitative PCR for M. hyopneumoniae DNA and routine bacteriological culture on bronchoalveolar lavage fluid Two weeks after challenge infection (D42), bronchoalveolar lavage fluid was collected from each pig by inserting a catheter (Portex® Dog Catheter with Female Luer Mount, Smiths Medical International Ltd., Kent, UK) in the trachea and flushing the lungs with 20 mL sterile PBS [ ]. .. Deoxyribonucleic acid was extracted from the BAL fluid using a commercial kit (DNeasy® Blood & Tissue kit, Qiagen, Venlo, The Netherlands) and a quantitative PCR (qPCR) was performed according to Marois et al. [ ] to measure the number of M. hyopneumoniae organisms.

    Article Title: Immunodominant, protective response to the parasite Toxoplasma gondii requires antigen processing in the endoplasmic reticulum
    Article Snippet: The number of infected splenocytes and peritoneal macrophages was determined by measurement of the percentage of GFP+ cells by flow cytometry. .. Genomic DNA was extracted from less than 10 mg ground tissue with the DNeasy Blood & Tissue kit (Qiagen).

    Expressing:

    Article Title: Highly sensitive detection of sentinel lymph node metastasis of breast cancer by digital PCR for RASSF1A methylation
    Article Snippet: DNA and mRNA were extracted from 10E+6 cells from each cell line using the DNeasy Blood & Tissue Kit and the RNeasy Mini Kit (both Qiagen GmbH), respectively. .. Briefly, 1 µg total RNA was reverse-transcribed into cDNA using the ReverTra Ace® qPCR RT kit (Toyobo Life Science), and CK19 mRNA expression was assessed using the Light Cycler 480 Real-time PCR System (Roche Applied Science) with the following conditions: 95°C for 10 min, followed by 50 cycles at 95°C for 15 sec and 60°C for 60 sec, with a final cycle at 50°C for 10 sec. KRT19 (CK19) TaqMan® Gene Expression Assays (Hs01051611_gH; Applied Biosystems; Thermo Fisher Scientific, Inc.) were used to conduct real-time PCR.

    Article Title: Expression quantitative trait loci detected in cell lines are often present in primary tissues
    Article Snippet: Given we are primarily interested in the effect of cis regulatory variation, and not the genetic basis of expression covariation among tissues, having largely non-overlapping sets of individuals is preferable to having all tissues from each individual because non-overlapping sets will reduce the impact of individual-specific effects, such as environmental effects, which otherwise could confound the analysis. .. We extracted DNA from each tissue using the Qiagen DNeasy Blood & Tissue Kit (Qiagen, Germantown, MD).

    DNA Methylation Assay:

    Article Title: Dynamic Imprinting of the Treg Cell-Specific Epigenetic Signature in Developing Thymic Regulatory T Cells
    Article Snippet: .. DNA Methylation Analysis Genomic DNA was isolated from sorted CD4SP thymocyte subsets using the DNeasy® Blood & Tissue Kit (Qiagen) and concentrated using the DNA Clean & Concentrator Kit (Zymo Research), both following the manufacturers' protocols. .. The DNA concentration was quantified with a Nanodrop 1000 spectrophotometer (Peqlab).

    Flow Cytometry:

    Article Title: Immunodominant, protective response to the parasite Toxoplasma gondii requires antigen processing in the endoplasmic reticulum
    Article Snippet: The number of infected splenocytes and peritoneal macrophages was determined by measurement of the percentage of GFP+ cells by flow cytometry. .. Genomic DNA was extracted from less than 10 mg ground tissue with the DNeasy Blood & Tissue kit (Qiagen).

    Cell Culture:

    Article Title: Highly sensitive detection of sentinel lymph node metastasis of breast cancer by digital PCR for RASSF1A methylation
    Article Snippet: Quantification of methylated RASSF1A and CK19 mRNA in breast cancer cell lines A total of six breast cancer cell lines (MCF7, MDA-MB-361, BT474, MDA-MB-453, MDA-MB-231 and BT20) were cultured in DMEM (Sigma-Aldrich; Merck KGaA), and five (ZR75-1, T47D, ZR75-30, SKBR3 and AU565) were cultured in RPMI-1640 (Sigma-Aldrich; Merck KGaA) at 37°C (5% CO2 ) in a humidified atmosphere. .. DNA and mRNA were extracted from 10E+6 cells from each cell line using the DNeasy Blood & Tissue Kit and the RNeasy Mini Kit (both Qiagen GmbH), respectively.

    Article Title: Establishment of a novel human CIC-DUX4 sarcoma cell line, Kitra-SRS, with autocrine IGF-1R activation and metastatic potential to the lungs
    Article Snippet: .. Authentication and quality control of the established cell line Genomic DNA was extracted from the tumour tissues or cultured cells using the DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany). .. Authentication of Kitra-SRS cells was achieved by examining STRs at 16 loci using STR multiplex assays (PowerPlex® 16 HS System; Promega, Madison, WI, USA).

    DNA Sequencing:

    Article Title: Diversity, Activity, and Evolution of CRISPR Loci in Streptococcus thermophilus ▿ ▿ †
    Article Snippet: Paragraph title: DNA sequencing of S. thermophilus CRISPR loci. ... S. thermophilus genomic DNA was extracted and purified by using a DNeasy Blood & Tissue kit (Qiagen).

    Sequencing:

    Article Title: A human expression system based on HEK293 for the stable production of recombinant erythropoietin
    Article Snippet: .. Sequencing of targeted GLUL genomic locus Genomic DNA was isolated from potential GLUL -KO cell lines using DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany). .. PCR using KOD Hot-start (Merck-Novagen, Darmstadt, Germany) was then performed to amplify GLUL with primers F: 5′-CTCCAGAACACCTTCCACCA-3′ and R: 5′-ACATTGCTGTCTCACCTTCC-3′.

    Article Title: Diversity, Activity, and Evolution of CRISPR Loci in Streptococcus thermophilus ▿ ▿ †
    Article Snippet: S. thermophilus genomic DNA was extracted and purified by using a DNeasy Blood & Tissue kit (Qiagen). .. Prior to sequencing, each CRISPR locus was amplified by PCR with LA-Taq DNA polymerase (Takara/Lonza).

    Sonication:

    Article Title: Prognostic implications of 5-hydroxymethylcytosines from circulating cell-free DNA in diffuse large B-cell lymphoma
    Article Snippet: .. Genomic DNA from cfDNA-paired tumor blocks for 7 patients was isolated (30-50 ng per sample) using a DNeasy Blood & Tissue Kit (Qiagen) and fragmented by sonication. .. We constructed 5hmC-Seal libraries according to an established protocol.

    DNA Extraction:

    Article Title: Application of different DNA extraction procedures, library preparation protocols and sequencing platforms: impact on sequencing results
    Article Snippet: .. Pellets obtained from cultures BACT1 to BACT4 were suspended in 180 μl of ATL buffer and submitted to DNA extraction using the DNeasy Blood & Tissue Kit (Qiagen, Milan, Italy). .. Pellets obtained from cultures BACT5 and BACT6 were suspended in 100 μl of R4 buffer and submitted to DNA extraction using the ChargeSwitch® gDNA Mini Bacteria Kit (Invitrogen, Milan, Italy).

    Article Title: Ancient mitogenomics clarifies radiation of extinct Mascarene giant tortoises (Cylindraspis spp.)
    Article Snippet: Negative controls (water blanks) were included during DNA extraction and library preparation and screened for evidence of contamination. .. DNA of the historic tissue sample was extracted using the DNeasy Blood & Tissue Kit (Qiagen).

    Methylation:

    Article Title: Highly sensitive detection of sentinel lymph node metastasis of breast cancer by digital PCR for RASSF1A methylation
    Article Snippet: Paragraph title: Quantification of methylated RASSF1A and CK19 mRNA in breast cancer cell lines ... DNA and mRNA were extracted from 10E+6 cells from each cell line using the DNeasy Blood & Tissue Kit and the RNeasy Mini Kit (both Qiagen GmbH), respectively.

    Article Title: Dynamic Imprinting of the Treg Cell-Specific Epigenetic Signature in Developing Thymic Regulatory T Cells
    Article Snippet: DNA Methylation Analysis Genomic DNA was isolated from sorted CD4SP thymocyte subsets using the DNeasy® Blood & Tissue Kit (Qiagen) and concentrated using the DNA Clean & Concentrator Kit (Zymo Research), both following the manufacturers' protocols. .. Methylation analysis of the TSDR and other Treg cell-specific epigenetic signature genes was performed using bisulfite sequencing as described before ( ).

    Multiple Displacement Amplification:

    Article Title: Highly sensitive detection of sentinel lymph node metastasis of breast cancer by digital PCR for RASSF1A methylation
    Article Snippet: Quantification of methylated RASSF1A and CK19 mRNA in breast cancer cell lines A total of six breast cancer cell lines (MCF7, MDA-MB-361, BT474, MDA-MB-453, MDA-MB-231 and BT20) were cultured in DMEM (Sigma-Aldrich; Merck KGaA), and five (ZR75-1, T47D, ZR75-30, SKBR3 and AU565) were cultured in RPMI-1640 (Sigma-Aldrich; Merck KGaA) at 37°C (5% CO2 ) in a humidified atmosphere. .. DNA and mRNA were extracted from 10E+6 cells from each cell line using the DNeasy Blood & Tissue Kit and the RNeasy Mini Kit (both Qiagen GmbH), respectively.

    Isolation:

    Article Title: A human expression system based on HEK293 for the stable production of recombinant erythropoietin
    Article Snippet: .. Sequencing of targeted GLUL genomic locus Genomic DNA was isolated from potential GLUL -KO cell lines using DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany). .. PCR using KOD Hot-start (Merck-Novagen, Darmstadt, Germany) was then performed to amplify GLUL with primers F: 5′-CTCCAGAACACCTTCCACCA-3′ and R: 5′-ACATTGCTGTCTCACCTTCC-3′.

    Article Title: HIF-1α protects against oxidative stress by directly targeting mitochondria
    Article Snippet: .. 2.6 Mitochondrial DNA amplification Total cellular DNA was isolated using DNeasy Blood & Tissue Kit (Qiagen, Dusseldorf, Germany) and quantified using a NanoDrop2000 spectrophotometer (Bio-Tek). .. Primers were designed using NCBI Primer-BLAST and synthesized by Life Technologies (Shanghai, China).

    Article Title: Dynamic Imprinting of the Treg Cell-Specific Epigenetic Signature in Developing Thymic Regulatory T Cells
    Article Snippet: .. DNA Methylation Analysis Genomic DNA was isolated from sorted CD4SP thymocyte subsets using the DNeasy® Blood & Tissue Kit (Qiagen) and concentrated using the DNA Clean & Concentrator Kit (Zymo Research), both following the manufacturers' protocols. .. The DNA concentration was quantified with a Nanodrop 1000 spectrophotometer (Peqlab).

    Article Title: Prognostic implications of 5-hydroxymethylcytosines from circulating cell-free DNA in diffuse large B-cell lymphoma
    Article Snippet: .. Genomic DNA from cfDNA-paired tumor blocks for 7 patients was isolated (30-50 ng per sample) using a DNeasy Blood & Tissue Kit (Qiagen) and fragmented by sonication. .. We constructed 5hmC-Seal libraries according to an established protocol.

    Size-exclusion Chromatography:

    Article Title: Highly sensitive detection of sentinel lymph node metastasis of breast cancer by digital PCR for RASSF1A methylation
    Article Snippet: DNA and mRNA were extracted from 10E+6 cells from each cell line using the DNeasy Blood & Tissue Kit and the RNeasy Mini Kit (both Qiagen GmbH), respectively. .. Briefly, 1 µg total RNA was reverse-transcribed into cDNA using the ReverTra Ace® qPCR RT kit (Toyobo Life Science), and CK19 mRNA expression was assessed using the Light Cycler 480 Real-time PCR System (Roche Applied Science) with the following conditions: 95°C for 10 min, followed by 50 cycles at 95°C for 15 sec and 60°C for 60 sec, with a final cycle at 50°C for 10 sec. KRT19 (CK19) TaqMan® Gene Expression Assays (Hs01051611_gH; Applied Biosystems; Thermo Fisher Scientific, Inc.) were used to conduct real-time PCR.

    Article Title: Ancient mitogenomics clarifies radiation of extinct Mascarene giant tortoises (Cylindraspis spp.)
    Article Snippet: DNA of the historic tissue sample was extracted using the DNeasy Blood & Tissue Kit (Qiagen). .. Bone fragments were crushed in a bone mill (Retsch MM400) for 10 sec at 20× /min or in a porcelain mortar by hand.

    Mouse Assay:

    Article Title: Dynamic Imprinting of the Treg Cell-Specific Epigenetic Signature in Developing Thymic Regulatory T Cells
    Article Snippet: DNA Methylation Analysis Genomic DNA was isolated from sorted CD4SP thymocyte subsets using the DNeasy® Blood & Tissue Kit (Qiagen) and concentrated using the DNA Clean & Concentrator Kit (Zymo Research), both following the manufacturers' protocols. .. Exclusively, cells from male mice were used for the methylation analysis.

    Polymerase Chain Reaction:

    Article Title: Highly sensitive detection of sentinel lymph node metastasis of breast cancer by digital PCR for RASSF1A methylation
    Article Snippet: DNA and mRNA were extracted from 10E+6 cells from each cell line using the DNeasy Blood & Tissue Kit and the RNeasy Mini Kit (both Qiagen GmbH), respectively. .. The relative CK19 mRNA expression level per cell was obtained, and a PCR product was used as the standard.

    Article Title: A human expression system based on HEK293 for the stable production of recombinant erythropoietin
    Article Snippet: Sequencing of targeted GLUL genomic locus Genomic DNA was isolated from potential GLUL -KO cell lines using DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany). .. PCR using KOD Hot-start (Merck-Novagen, Darmstadt, Germany) was then performed to amplify GLUL with primers F: 5′-CTCCAGAACACCTTCCACCA-3′ and R: 5′-ACATTGCTGTCTCACCTTCC-3′.

    Article Title: HIF-1α protects against oxidative stress by directly targeting mitochondria
    Article Snippet: 2.6 Mitochondrial DNA amplification Total cellular DNA was isolated using DNeasy Blood & Tissue Kit (Qiagen, Dusseldorf, Germany) and quantified using a NanoDrop2000 spectrophotometer (Bio-Tek). .. For each primer pair, the annealing temperature was optimized by gradient PCR.

    Article Title: Taxonomic revision of the endemic Cameroonian freshwater crab genus Louisea Cumberlidge, 1994 ( Crustacea, Decapoda, Brachyura, Potamonautidae), with descriptions of two new species from Nkongsamba and Yabassi
    Article Snippet: Genomic DNA was extracted from a tissue sample of up to 25 mg cut from the pereiopod muscle of 70% ethanol-preserved specimens using the Qiagen DNeasy Blood & Tissue kit. .. Polymerase chain reaction (PCR) was used to amplify three mitochondrial gene fragments, a ~ 638 bp region of the 16S ribosomal RNA gene (16S) using primers 16L29 and 16HLeu or 16H10 , a ~ 594 bp region of the 12S ribosomal RNA gene (12S) using primers 12L4 and 12H2 , and a 648 bp fragment of the Cytochrome Oxidase subunit I gene (COI) using primers COL6a ( ) and COH1b , COH6 , or CO1a ( ).

    Article Title: Immunodominant, protective response to the parasite Toxoplasma gondii requires antigen processing in the endoplasmic reticulum
    Article Snippet: Parasite burden in spleen, liver and brain was assessed by semiquantitative PCR as described . .. Genomic DNA was extracted from less than 10 mg ground tissue with the DNeasy Blood & Tissue kit (Qiagen).

    Article Title: Diversity, Activity, and Evolution of CRISPR Loci in Streptococcus thermophilus ▿ ▿ †
    Article Snippet: S. thermophilus genomic DNA was extracted and purified by using a DNeasy Blood & Tissue kit (Qiagen). .. Prior to sequencing, each CRISPR locus was amplified by PCR with LA-Taq DNA polymerase (Takara/Lonza).

    Construct:

    Article Title: Prognostic implications of 5-hydroxymethylcytosines from circulating cell-free DNA in diffuse large B-cell lymphoma
    Article Snippet: Genomic DNA from cfDNA-paired tumor blocks for 7 patients was isolated (30-50 ng per sample) using a DNeasy Blood & Tissue Kit (Qiagen) and fragmented by sonication. .. We constructed 5hmC-Seal libraries according to an established protocol.

    CRISPR:

    Article Title: Diversity, Activity, and Evolution of CRISPR Loci in Streptococcus thermophilus ▿ ▿ †
    Article Snippet: Paragraph title: DNA sequencing of S. thermophilus CRISPR loci. ... S. thermophilus genomic DNA was extracted and purified by using a DNeasy Blood & Tissue kit (Qiagen).

    Purification:

    Article Title: Diversity, Activity, and Evolution of CRISPR Loci in Streptococcus thermophilus ▿ ▿ †
    Article Snippet: .. S. thermophilus genomic DNA was extracted and purified by using a DNeasy Blood & Tissue kit (Qiagen). .. Prior to sequencing, each CRISPR locus was amplified by PCR with LA-Taq DNA polymerase (Takara/Lonza).

    Chromatin Immunoprecipitation:

    Article Title: The Genetic Architecture of Bovine Telomere Length in Early Life and Association With Animal Fitness
    Article Snippet: Genotyping DNA was extracted from whole blood using the DNeasy Blood & Tissue kit (QIAGEN) as described in . .. Animals used in the presented study had been previously genotyped under the following platforms: - Illumina BovineSNP50 BeadChip (Illumina Inc., San Diego, CA)—246 animals - GGP Bovine 50K (Neogen/GeneSeek)—161 animals - GGP Bovine HD 150K (Neogen/GeneSeek)—295 animals The genotypes obtained from the GGP chips 50K and 150K were subsequently imputed to the SNPs found on the 50K Illumina chip using the method of and the software FindHap.

    Software:

    Article Title: The Genetic Architecture of Bovine Telomere Length in Early Life and Association With Animal Fitness
    Article Snippet: Genotyping DNA was extracted from whole blood using the DNeasy Blood & Tissue kit (QIAGEN) as described in . .. Animals used in the presented study had been previously genotyped under the following platforms: - Illumina BovineSNP50 BeadChip (Illumina Inc., San Diego, CA)—246 animals - GGP Bovine 50K (Neogen/GeneSeek)—161 animals - GGP Bovine HD 150K (Neogen/GeneSeek)—295 animals The genotypes obtained from the GGP chips 50K and 150K were subsequently imputed to the SNPs found on the 50K Illumina chip using the method of and the software FindHap.

    Multiplex Assay:

    Article Title: Establishment of a novel human CIC-DUX4 sarcoma cell line, Kitra-SRS, with autocrine IGF-1R activation and metastatic potential to the lungs
    Article Snippet: Authentication and quality control of the established cell line Genomic DNA was extracted from the tumour tissues or cultured cells using the DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany). .. Authentication of Kitra-SRS cells was achieved by examining STRs at 16 loci using STR multiplex assays (PowerPlex® 16 HS System; Promega, Madison, WI, USA).

    Sample Prep:

    Article Title: Prognostic implications of 5-hydroxymethylcytosines from circulating cell-free DNA in diffuse large B-cell lymphoma
    Article Snippet: Paragraph title: Sample preparation and the 5hmC-Seal profiling ... Genomic DNA from cfDNA-paired tumor blocks for 7 patients was isolated (30-50 ng per sample) using a DNeasy Blood & Tissue Kit (Qiagen) and fragmented by sonication.

    Spectrophotometry:

    Article Title: HIF-1α protects against oxidative stress by directly targeting mitochondria
    Article Snippet: .. 2.6 Mitochondrial DNA amplification Total cellular DNA was isolated using DNeasy Blood & Tissue Kit (Qiagen, Dusseldorf, Germany) and quantified using a NanoDrop2000 spectrophotometer (Bio-Tek). .. Primers were designed using NCBI Primer-BLAST and synthesized by Life Technologies (Shanghai, China).

    Article Title: Dynamic Imprinting of the Treg Cell-Specific Epigenetic Signature in Developing Thymic Regulatory T Cells
    Article Snippet: DNA Methylation Analysis Genomic DNA was isolated from sorted CD4SP thymocyte subsets using the DNeasy® Blood & Tissue Kit (Qiagen) and concentrated using the DNA Clean & Concentrator Kit (Zymo Research), both following the manufacturers' protocols. .. The DNA concentration was quantified with a Nanodrop 1000 spectrophotometer (Peqlab).

    Sampling:

    Article Title: Ancient mitogenomics clarifies radiation of extinct Mascarene giant tortoises (Cylindraspis spp.)
    Article Snippet: Processing of historic and subfossil Cylindraspis samples The 18 subfossil bone specimens were sampled using a Dremel drill equipped either with a core sampling bit or diamond circular saw bit, depending on the specimen. .. DNA of the historic tissue sample was extracted using the DNeasy Blood & Tissue Kit (Qiagen).

    Concentration Assay:

    Article Title: Dynamic Imprinting of the Treg Cell-Specific Epigenetic Signature in Developing Thymic Regulatory T Cells
    Article Snippet: DNA Methylation Analysis Genomic DNA was isolated from sorted CD4SP thymocyte subsets using the DNeasy® Blood & Tissue Kit (Qiagen) and concentrated using the DNA Clean & Concentrator Kit (Zymo Research), both following the manufacturers' protocols. .. The DNA concentration was quantified with a Nanodrop 1000 spectrophotometer (Peqlab).

    Gel Extraction:

    Article Title: A human expression system based on HEK293 for the stable production of recombinant erythropoietin
    Article Snippet: Sequencing of targeted GLUL genomic locus Genomic DNA was isolated from potential GLUL -KO cell lines using DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany). .. PCR products were run on a gel where single bands obtained were extracted using Gel Extraction Kit (Machery-Nagel, Düren, Germany).

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    Qiagen dneasy blood tissue kit
    Dneasy Blood Tissue Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1747 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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