dneasy blood and tissue kit  (Qiagen)


Bioz Verified Symbol Qiagen is a verified supplier
Bioz Manufacturer Symbol Qiagen manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    Name:
    DNeasy Blood Tissue Kit
    Description:
    For spin column or 96 well purification of total DNA from animal blood and tissues and from cells yeast bacteria or viruses Kit contents Qiagen DNeasy Blood Tissue Kit 50 preps 100L 25mg Sample 100 to 200L Elution Volume Blood Tissue Sample Total DNA Purification Silica Technology Spin Column Format 6g 30g Yield Manual Processing For Purification of Total DNA from Animal Blood and Tissues and from Cells Yeast Bacteria or Viruses Ideal for PCR Real time PCR Genotyping Includes 50 DNeasy Mini Spin Columns Proteinase K Buffers 2mL Collection Tubes Benefits Standardized method for a variety of sample types High yields even from specialized samples High quality DNA Optimized protocols for a range of starting materials Spin column and 96 well high throughput form
    Catalog Number:
    69504
    Price:
    163
    Category:
    DNeasy Blood Tissue Kits
    Buy from Supplier


    Structured Review

    Qiagen dneasy blood and tissue kit
    DNeasy Blood Tissue Kit
    For spin column or 96 well purification of total DNA from animal blood and tissues and from cells yeast bacteria or viruses Kit contents Qiagen DNeasy Blood Tissue Kit 50 preps 100L 25mg Sample 100 to 200L Elution Volume Blood Tissue Sample Total DNA Purification Silica Technology Spin Column Format 6g 30g Yield Manual Processing For Purification of Total DNA from Animal Blood and Tissues and from Cells Yeast Bacteria or Viruses Ideal for PCR Real time PCR Genotyping Includes 50 DNeasy Mini Spin Columns Proteinase K Buffers 2mL Collection Tubes Benefits Standardized method for a variety of sample types High yields even from specialized samples High quality DNA Optimized protocols for a range of starting materials Spin column and 96 well high throughput form
    https://www.bioz.com/result/dneasy blood and tissue kit/product/Qiagen
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dneasy blood and tissue kit - by Bioz Stars, 2021-03
    97/100 stars

    Images

    1) Product Images from "Comparison of DNA extraction methods for non-marine molluscs: Is modified CTAB DNA extraction method more efficient than DNA extraction kits?"

    Article Title: Comparison of DNA extraction methods for non-marine molluscs: Is modified CTAB DNA extraction method more efficient than DNA extraction kits?

    Journal: bioRxiv

    doi: 10.1101/863167

    Comparison of average sequence quality between CTAB and Qiagen® DNeasy Blood and Tissue Kit extracted amplified genomic DNA
    Figure Legend Snippet: Comparison of average sequence quality between CTAB and Qiagen® DNeasy Blood and Tissue Kit extracted amplified genomic DNA

    Techniques Used: Sequencing, Amplification

    Chromatogram depicting 16S gene sequence quality in Qiagen ® DNeasy Blood and Tissue Kit extracted DNA.
    Figure Legend Snippet: Chromatogram depicting 16S gene sequence quality in Qiagen ® DNeasy Blood and Tissue Kit extracted DNA.

    Techniques Used: Sequencing

    2) Product Images from "Development of real-time PCR for detection of Mycoplasma hominis"

    Article Title: Development of real-time PCR for detection of Mycoplasma hominis

    Journal: BMC Microbiology

    doi: 10.1186/1471-2180-4-35

    DNeasy treated samples. (a) Two LightCycler PCR runs, first one with standard dilution series of human Hep2 DNA before (flat negative curves) and after DNeasy (indicated with squares and triangles), showed on the left and second with DNA free water before (marked with squares) and after DNeasy (triangles) on the right. (b) Melting curve analysis of DNeasy treated H 2 O (triangles), Hep2 DNA (squares) and clinical sample.
    Figure Legend Snippet: DNeasy treated samples. (a) Two LightCycler PCR runs, first one with standard dilution series of human Hep2 DNA before (flat negative curves) and after DNeasy (indicated with squares and triangles), showed on the left and second with DNA free water before (marked with squares) and after DNeasy (triangles) on the right. (b) Melting curve analysis of DNeasy treated H 2 O (triangles), Hep2 DNA (squares) and clinical sample.

    Techniques Used: Polymerase Chain Reaction

    3) Product Images from "A Novel Molecular Test to Diagnose Canine Visceral Leishmaniasis at the Point of Care"

    Article Title: A Novel Molecular Test to Diagnose Canine Visceral Leishmaniasis at the Point of Care

    Journal: The American Journal of Tropical Medicine and Hygiene

    doi: 10.4269/ajtmh.15-0145

    Sensitivity of recombinase polymerase amplification–lateral flow (RPA-LF) to detect Leishmania infantum compared with real-time polymerase chain reaction (PCR) used as gold standard. Tenfold serial dilutions of L. infantum promastigotes in dog blood were extracted using Qiagen ® DNeasy blood and tissue kit and detected by real-time quantitative PCR (SYBRgreen) or RPA-LF. Parasite dilutions: 1 = 10 5 , 2 = 10 4 , 3 = 10 3 , 4 = 10 2 , 5 = 10, 6 = 1, and 7 = 0.1 parasites and Bl = uninfected dog blood. The top band is the control band; the lower band is the test band. This is a representative figure of two similar assays.
    Figure Legend Snippet: Sensitivity of recombinase polymerase amplification–lateral flow (RPA-LF) to detect Leishmania infantum compared with real-time polymerase chain reaction (PCR) used as gold standard. Tenfold serial dilutions of L. infantum promastigotes in dog blood were extracted using Qiagen ® DNeasy blood and tissue kit and detected by real-time quantitative PCR (SYBRgreen) or RPA-LF. Parasite dilutions: 1 = 10 5 , 2 = 10 4 , 3 = 10 3 , 4 = 10 2 , 5 = 10, 6 = 1, and 7 = 0.1 parasites and Bl = uninfected dog blood. The top band is the control band; the lower band is the test band. This is a representative figure of two similar assays.

    Techniques Used: Recombinase Polymerase Amplification, Flow Cytometry, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction

    4) Product Images from "Tissue Fractionation by Hydrostatic Pressure Cycling Technology: The Unified Sample Preparation Technique for Systems Biology Studies"

    Article Title: Tissue Fractionation by Hydrostatic Pressure Cycling Technology: The Unified Sample Preparation Technique for Systems Biology Studies

    Journal: Journal of Biomolecular Techniques : JBT

    doi:

    A: DNA and protein recovery from the cell culture. one aliquot of cells was processed with PCT and ProteoSolve-SB (P). DNA was extracted from the solid phase using the DNeasy kit. The control aliquot of cells was processed directly with the qiagen DNeasy
    Figure Legend Snippet: A: DNA and protein recovery from the cell culture. one aliquot of cells was processed with PCT and ProteoSolve-SB (P). DNA was extracted from the solid phase using the DNeasy kit. The control aliquot of cells was processed directly with the qiagen DNeasy

    Techniques Used: Cell Culture

    5) Product Images from "The Anatomy to Genomics (ATG) Start Genetics medical school initiative: incorporating exome sequencing data from cadavers used for Anatomy instruction into the first year curriculum"

    Article Title: The Anatomy to Genomics (ATG) Start Genetics medical school initiative: incorporating exome sequencing data from cadavers used for Anatomy instruction into the first year curriculum

    Journal: BMC Medical Genomics

    doi: 10.1186/s12920-016-0223-4

    Agarose gel electrophoresis of DNA isolated from cadaver tissues using the DNeasy Blood Tissue Kit. MW = Quick-load 1 kb DNA Ladder (New England BioLabs). Lane 1: Liver without RNAse treatment. Lane 2: Heart without RNAse treatment. Lane 3: Liver with RNAse treatment. Lane 4: Heart with RNAse treatment. Lane 5: Skeletal Muscle with RNAse treatment. Lane 6: Skin with RNAse treatment
    Figure Legend Snippet: Agarose gel electrophoresis of DNA isolated from cadaver tissues using the DNeasy Blood Tissue Kit. MW = Quick-load 1 kb DNA Ladder (New England BioLabs). Lane 1: Liver without RNAse treatment. Lane 2: Heart without RNAse treatment. Lane 3: Liver with RNAse treatment. Lane 4: Heart with RNAse treatment. Lane 5: Skeletal Muscle with RNAse treatment. Lane 6: Skin with RNAse treatment

    Techniques Used: Agarose Gel Electrophoresis, Isolation

    Agarose gel electrophoresis of DNA isolated from cadaver tissues. MW = GeneRuler 1 kb Plus DNA Ladder (Thermo Fisher Scientific). Lane 1: Heart DNA extracted with DNeasy Blood Tissue Kit. Lane 2: Liver DNA extracted with DNeasy Blood Tissue Kit. Lane 3: Heart DNA extracted with FFPE kit. Lane 4: Liver DNA extracted with FFPE kit
    Figure Legend Snippet: Agarose gel electrophoresis of DNA isolated from cadaver tissues. MW = GeneRuler 1 kb Plus DNA Ladder (Thermo Fisher Scientific). Lane 1: Heart DNA extracted with DNeasy Blood Tissue Kit. Lane 2: Liver DNA extracted with DNeasy Blood Tissue Kit. Lane 3: Heart DNA extracted with FFPE kit. Lane 4: Liver DNA extracted with FFPE kit

    Techniques Used: Agarose Gel Electrophoresis, Isolation, Formalin-fixed Paraffin-Embedded

    6) Product Images from "Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows, et al. Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows"

    Article Title: Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows, et al. Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows

    Journal: Ecology and Evolution

    doi: 10.1002/ece3.6540

    Detection rates of the twelve tested workflows for water samples (a) and sediment samples (b), shown is the percentage of positive samples among the 16 PCR reactions of each workflow (CB/ C = CTAB DNA extraction, DN = DNeasy Blood Tissue Kit, HS/ H = high salt DNA extraction, N = Nucleo Spin Soil Kit)
    Figure Legend Snippet: Detection rates of the twelve tested workflows for water samples (a) and sediment samples (b), shown is the percentage of positive samples among the 16 PCR reactions of each workflow (CB/ C = CTAB DNA extraction, DN = DNeasy Blood Tissue Kit, HS/ H = high salt DNA extraction, N = Nucleo Spin Soil Kit)

    Techniques Used: Polymerase Chain Reaction, DNA Extraction

    Total DNA yield (a) and threshold cycle values (b) measured in water samples of the twelve tested workflows (CB = CTAB DNA extraction, DN = DNeasy Blood Tissue Kit, HS = high salt DNA extraction). C t values are inversely proportional to the target DNA amount of a sample; thus, the lower the C t value, the higher is the target DNA amount
    Figure Legend Snippet: Total DNA yield (a) and threshold cycle values (b) measured in water samples of the twelve tested workflows (CB = CTAB DNA extraction, DN = DNeasy Blood Tissue Kit, HS = high salt DNA extraction). C t values are inversely proportional to the target DNA amount of a sample; thus, the lower the C t value, the higher is the target DNA amount

    Techniques Used: DNA Extraction

    7) Product Images from "Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows, et al. Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows"

    Article Title: Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows, et al. Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows

    Journal: Ecology and Evolution

    doi: 10.1002/ece3.6540

    Detection rates of the twelve tested workflows for water samples (a) and sediment samples (b), shown is the percentage of positive samples among the 16 PCR reactions of each workflow (CB/ C = CTAB DNA extraction, DN = DNeasy Blood Tissue Kit, HS/ H = high salt DNA extraction, N = Nucleo Spin Soil Kit)
    Figure Legend Snippet: Detection rates of the twelve tested workflows for water samples (a) and sediment samples (b), shown is the percentage of positive samples among the 16 PCR reactions of each workflow (CB/ C = CTAB DNA extraction, DN = DNeasy Blood Tissue Kit, HS/ H = high salt DNA extraction, N = Nucleo Spin Soil Kit)

    Techniques Used: Polymerase Chain Reaction, DNA Extraction

    Total DNA yield (a) and threshold cycle values (b) measured in water samples of the twelve tested workflows (CB = CTAB DNA extraction, DN = DNeasy Blood Tissue Kit, HS = high salt DNA extraction). C t values are inversely proportional to the target DNA amount of a sample; thus, the lower the C t value, the higher is the target DNA amount
    Figure Legend Snippet: Total DNA yield (a) and threshold cycle values (b) measured in water samples of the twelve tested workflows (CB = CTAB DNA extraction, DN = DNeasy Blood Tissue Kit, HS = high salt DNA extraction). C t values are inversely proportional to the target DNA amount of a sample; thus, the lower the C t value, the higher is the target DNA amount

    Techniques Used: DNA Extraction

    8) Product Images from "Comparison of DNA extraction methods for non-marine molluscs: Is modified CTAB DNA extraction method more efficient than DNA extraction kits?"

    Article Title: Comparison of DNA extraction methods for non-marine molluscs: Is modified CTAB DNA extraction method more efficient than DNA extraction kits?

    Journal: bioRxiv

    doi: 10.1101/863167

    Comparison of average sequence quality between CTAB and Qiagen® DNeasy Blood and Tissue Kit extracted amplified genomic DNA
    Figure Legend Snippet: Comparison of average sequence quality between CTAB and Qiagen® DNeasy Blood and Tissue Kit extracted amplified genomic DNA

    Techniques Used: Sequencing, Amplification

    Chromatogram depicting 16S gene sequence quality in Qiagen ® DNeasy Blood and Tissue Kit extracted DNA.
    Figure Legend Snippet: Chromatogram depicting 16S gene sequence quality in Qiagen ® DNeasy Blood and Tissue Kit extracted DNA.

    Techniques Used: Sequencing

    9) Product Images from "Absolute Enumeration of Probiotic Strains Lactobacillus acidophilus NCFM® and Bifidobacterium animalis subsp. lactis Bl-04® via Chip-Based Digital PCR"

    Article Title: Absolute Enumeration of Probiotic Strains Lactobacillus acidophilus NCFM® and Bifidobacterium animalis subsp. lactis Bl-04® via Chip-Based Digital PCR

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2018.00704

    Qiagen DNeasy replicate isolations of DNA from NCFM overnight cells not treated with PMA. Columns with differing letter are significantly different from each other.
    Figure Legend Snippet: Qiagen DNeasy replicate isolations of DNA from NCFM overnight cells not treated with PMA. Columns with differing letter are significantly different from each other.

    Techniques Used:

    10) Product Images from "Development of real-time PCR for detection of Mycoplasma hominis"

    Article Title: Development of real-time PCR for detection of Mycoplasma hominis

    Journal: BMC Microbiology

    doi: 10.1186/1471-2180-4-35

    DNeasy treated samples. (a) Two LightCycler PCR runs, first one with standard dilution series of human Hep2 DNA before (flat negative curves) and after DNeasy (indicated with squares and triangles), showed on the left and second with DNA free water before (marked with squares) and after DNeasy (triangles) on the right. (b) Melting curve analysis of DNeasy treated H 2 O (triangles), Hep2 DNA (squares) and clinical sample.
    Figure Legend Snippet: DNeasy treated samples. (a) Two LightCycler PCR runs, first one with standard dilution series of human Hep2 DNA before (flat negative curves) and after DNeasy (indicated with squares and triangles), showed on the left and second with DNA free water before (marked with squares) and after DNeasy (triangles) on the right. (b) Melting curve analysis of DNeasy treated H 2 O (triangles), Hep2 DNA (squares) and clinical sample.

    Techniques Used: Polymerase Chain Reaction

    11) Product Images from "Evaluation of Real-time PCR Based on SYBR Green I Fluorescent Dye for Detection of Bacillus Anthracis Strains in Biological Samples"

    Article Title: Evaluation of Real-time PCR Based on SYBR Green I Fluorescent Dye for Detection of Bacillus Anthracis Strains in Biological Samples

    Journal: Journal of Veterinary Research

    doi: 10.2478/jvetres-2018-0075

    Determination of the sensitivity of SYBR Green I real-time PCR with rpoB-F/R primers for the detection of B. anthracis strain 1/47 in liver (a) and blood (b) samples using a DNeasy Blood and Tissue Kit for DNA isolation
    Figure Legend Snippet: Determination of the sensitivity of SYBR Green I real-time PCR with rpoB-F/R primers for the detection of B. anthracis strain 1/47 in liver (a) and blood (b) samples using a DNeasy Blood and Tissue Kit for DNA isolation

    Techniques Used: SYBR Green Assay, Real-time Polymerase Chain Reaction, DNA Extraction

    Determination of the sensitivity of SYBR Green I real-time PCR with capC-F/R primers for the detection of B. anthracis strain 1/47 in liver (a) and blood (b) samples using a DNeasy Blood and Tissue Kit for DNA isolation
    Figure Legend Snippet: Determination of the sensitivity of SYBR Green I real-time PCR with capC-F/R primers for the detection of B. anthracis strain 1/47 in liver (a) and blood (b) samples using a DNeasy Blood and Tissue Kit for DNA isolation

    Techniques Used: SYBR Green Assay, Real-time Polymerase Chain Reaction, DNA Extraction

    12) Product Images from "Biocidal Efficacy of Copper Alloys against Pathogenic Enterococci Involves Degradation of Genomic and Plasmid DNAs ▿"

    Article Title: Biocidal Efficacy of Copper Alloys against Pathogenic Enterococci Involves Degradation of Genomic and Plasmid DNAs ▿

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.03050-09

    Agarose gel electrophoresis of purified enterococcal DNA. (A) Purified genomic DNA of E. faecium NCTC 12202. Lane 2, cells not exposed to metal surfaces; lane 3, cells exposed to stainless steel for 2 h; lane 4, cells exposed to copper for 2 h; lane 5, cells exposed to copper for 4 h. (B) Purified genomic DNA of clinical isolates E. faecium 5 (lanes 7 and 8) and E. faecalis 2 (lanes 9 and 10) exposed to stainless steel (lanes 7 and 9) or copper (lanes 8 and 10) for 2 h at 22°C. (C) Purified plasmid DNA of E. faecium NCTC 12202 not exposed to metal (lane 11) or exposed to stainless steel (lane 12) or copper (lane 13) for 2 h at 22°C. Control lanes are Bioline Hyperladder I (lanes 1) and Hyperladder II (lanes 6). Genomic DNA was purified using the Qiagen DNeasy Blood and Tissue kit (2% agarose) and the Qiaprep Spin Miniprep kit for plasmid DNA (0.9% agarose).
    Figure Legend Snippet: Agarose gel electrophoresis of purified enterococcal DNA. (A) Purified genomic DNA of E. faecium NCTC 12202. Lane 2, cells not exposed to metal surfaces; lane 3, cells exposed to stainless steel for 2 h; lane 4, cells exposed to copper for 2 h; lane 5, cells exposed to copper for 4 h. (B) Purified genomic DNA of clinical isolates E. faecium 5 (lanes 7 and 8) and E. faecalis 2 (lanes 9 and 10) exposed to stainless steel (lanes 7 and 9) or copper (lanes 8 and 10) for 2 h at 22°C. (C) Purified plasmid DNA of E. faecium NCTC 12202 not exposed to metal (lane 11) or exposed to stainless steel (lane 12) or copper (lane 13) for 2 h at 22°C. Control lanes are Bioline Hyperladder I (lanes 1) and Hyperladder II (lanes 6). Genomic DNA was purified using the Qiagen DNeasy Blood and Tissue kit (2% agarose) and the Qiaprep Spin Miniprep kit for plasmid DNA (0.9% agarose).

    Techniques Used: Agarose Gel Electrophoresis, Purification, Plasmid Preparation

    13) Product Images from "Methods to maximise recovery of environmental DNA from water samples"

    Article Title: Methods to maximise recovery of environmental DNA from water samples

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0179251

    Experimental design for Experiment 2A to investigate which DNA extraction kit-filter paper combination would give the best DNA yield. N refers to number of technical replicates. CN, MCE, PES, PCTE and GF refer to the types of filter papers whereas DNeasy and PowerWater (PW) refer to the two DNA extraction kits used in the experiment.
    Figure Legend Snippet: Experimental design for Experiment 2A to investigate which DNA extraction kit-filter paper combination would give the best DNA yield. N refers to number of technical replicates. CN, MCE, PES, PCTE and GF refer to the types of filter papers whereas DNeasy and PowerWater (PW) refer to the two DNA extraction kits used in the experiment.

    Techniques Used: DNA Extraction

    DNA yield from eight DNA extraction kit-filter paper combinations from samples in aquaria with UV-sterilized water. Clear bars used DNeasy extraction kit while shaded grey bars used the PowerWater kit. CN = Cellulose Nitrate, MCE = Mixed Cellulose Ester, PES = Polyethersulfone, PCTE = Polycarbonate track-etched. Error bars show the ±2 standard deviation of the mean.
    Figure Legend Snippet: DNA yield from eight DNA extraction kit-filter paper combinations from samples in aquaria with UV-sterilized water. Clear bars used DNeasy extraction kit while shaded grey bars used the PowerWater kit. CN = Cellulose Nitrate, MCE = Mixed Cellulose Ester, PES = Polyethersulfone, PCTE = Polycarbonate track-etched. Error bars show the ±2 standard deviation of the mean.

    Techniques Used: DNA Extraction, Standard Deviation

    Experimental design for Experiment 2B investigating DNA yields in five different DNA extraction kit-filter paper combination from stream water. N refers to number of technical replicates. CN, MCE, PES, PCTE and GF refer to the types of filter papers whereas DNeasy and PowerWater (PW) refer to the two DNA extraction kits used in the experiment.
    Figure Legend Snippet: Experimental design for Experiment 2B investigating DNA yields in five different DNA extraction kit-filter paper combination from stream water. N refers to number of technical replicates. CN, MCE, PES, PCTE and GF refer to the types of filter papers whereas DNeasy and PowerWater (PW) refer to the two DNA extraction kits used in the experiment.

    Techniques Used: DNA Extraction

    Experimental design used to compare five different combinations of capture, preservation and DNA extraction methods. N is the number of technical replicates and each replicate represents one 250 ml water sample. DNeasy and PowerWater (PW) refer to the two DNA extraction kits used in the experiment.
    Figure Legend Snippet: Experimental design used to compare five different combinations of capture, preservation and DNA extraction methods. N is the number of technical replicates and each replicate represents one 250 ml water sample. DNeasy and PowerWater (PW) refer to the two DNA extraction kits used in the experiment.

    Techniques Used: Preserving, DNA Extraction

    DNA yield from five different extraction kit-filter paper combinations from stream water samples. Clear bars used DNeasy extraction kit while shaded grey bars used the PowerWater kit. Error bars show the ±2 standard deviation of the mean.
    Figure Legend Snippet: DNA yield from five different extraction kit-filter paper combinations from stream water samples. Clear bars used DNeasy extraction kit while shaded grey bars used the PowerWater kit. Error bars show the ±2 standard deviation of the mean.

    Techniques Used: Standard Deviation

    14) Product Images from "Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows, et al. Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows"

    Article Title: Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows, et al. Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows

    Journal: Ecology and Evolution

    doi: 10.1002/ece3.6540

    Detection rates of the twelve tested workflows for water samples (a) and sediment samples (b), shown is the percentage of positive samples among the 16 PCR reactions of each workflow (CB/ C = CTAB DNA extraction, DN = DNeasy Blood Tissue Kit, HS/ H = high salt DNA extraction, N = Nucleo Spin Soil Kit)
    Figure Legend Snippet: Detection rates of the twelve tested workflows for water samples (a) and sediment samples (b), shown is the percentage of positive samples among the 16 PCR reactions of each workflow (CB/ C = CTAB DNA extraction, DN = DNeasy Blood Tissue Kit, HS/ H = high salt DNA extraction, N = Nucleo Spin Soil Kit)

    Techniques Used: Polymerase Chain Reaction, DNA Extraction

    Total DNA yield (a) and threshold cycle values (b) measured in water samples of the twelve tested workflows (CB = CTAB DNA extraction, DN = DNeasy Blood Tissue Kit, HS = high salt DNA extraction). C t values are inversely proportional to the target DNA amount of a sample; thus, the lower the C t value, the higher is the target DNA amount
    Figure Legend Snippet: Total DNA yield (a) and threshold cycle values (b) measured in water samples of the twelve tested workflows (CB = CTAB DNA extraction, DN = DNeasy Blood Tissue Kit, HS = high salt DNA extraction). C t values are inversely proportional to the target DNA amount of a sample; thus, the lower the C t value, the higher is the target DNA amount

    Techniques Used: DNA Extraction

    15) Product Images from "Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows, et al. Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows"

    Article Title: Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows, et al. Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows

    Journal: Ecology and Evolution

    doi: 10.1002/ece3.6540

    Detection rates of the twelve tested workflows for water samples (a) and sediment samples (b), shown is the percentage of positive samples among the 16 PCR reactions of each workflow (CB/ C = CTAB DNA extraction, DN = DNeasy Blood Tissue Kit, HS/ H = high salt DNA extraction, N = Nucleo Spin Soil Kit)
    Figure Legend Snippet: Detection rates of the twelve tested workflows for water samples (a) and sediment samples (b), shown is the percentage of positive samples among the 16 PCR reactions of each workflow (CB/ C = CTAB DNA extraction, DN = DNeasy Blood Tissue Kit, HS/ H = high salt DNA extraction, N = Nucleo Spin Soil Kit)

    Techniques Used: Polymerase Chain Reaction, DNA Extraction

    Total DNA yield (a) and threshold cycle values (b) measured in water samples of the twelve tested workflows (CB = CTAB DNA extraction, DN = DNeasy Blood Tissue Kit, HS = high salt DNA extraction). C t values are inversely proportional to the target DNA amount of a sample; thus, the lower the C t value, the higher is the target DNA amount
    Figure Legend Snippet: Total DNA yield (a) and threshold cycle values (b) measured in water samples of the twelve tested workflows (CB = CTAB DNA extraction, DN = DNeasy Blood Tissue Kit, HS = high salt DNA extraction). C t values are inversely proportional to the target DNA amount of a sample; thus, the lower the C t value, the higher is the target DNA amount

    Techniques Used: DNA Extraction

    16) Product Images from "16S rRNA gene pyrosequencing of reference and clinical samples and investigation of the temperature stability of microbiome profiles"

    Article Title: 16S rRNA gene pyrosequencing of reference and clinical samples and investigation of the temperature stability of microbiome profiles

    Journal: Microbiome

    doi: 10.1186/2049-2618-2-31

    Variability of oropharyngeal microbiome profiles with storage temperatures. (A) Variability of oropharyngeal microbiome profiles with storage temperatures for the MO BIO PowerSoil extraction method. Four specimens were collected from each subject and stored for 4 weeks at different temperatures. Microbiome profiles at the genus level were estimated using QIIME. The figure presents the principal coordinate analysis based on the weighted UniFrac distances between these microbiome profiles. Each coordinate axis explains the specified percent of the total community variability. (B) Variability of oropharyngeal microbiome profiles with storage temperatures for the Qiagen DNeasy extraction method. Four specimens were collected from each subject and stored for four weeks at different temperatures. Microbiome profiles at the genus level were estimated using QIIME. The figure presents the principal coordinate analysis based on the weighted UniFrac distances between these microbiome profiles. Each coordinate axis explains the specified percent of the total community variability.
    Figure Legend Snippet: Variability of oropharyngeal microbiome profiles with storage temperatures. (A) Variability of oropharyngeal microbiome profiles with storage temperatures for the MO BIO PowerSoil extraction method. Four specimens were collected from each subject and stored for 4 weeks at different temperatures. Microbiome profiles at the genus level were estimated using QIIME. The figure presents the principal coordinate analysis based on the weighted UniFrac distances between these microbiome profiles. Each coordinate axis explains the specified percent of the total community variability. (B) Variability of oropharyngeal microbiome profiles with storage temperatures for the Qiagen DNeasy extraction method. Four specimens were collected from each subject and stored for four weeks at different temperatures. Microbiome profiles at the genus level were estimated using QIIME. The figure presents the principal coordinate analysis based on the weighted UniFrac distances between these microbiome profiles. Each coordinate axis explains the specified percent of the total community variability.

    Techniques Used:

    Normalization of PCR amplification by choosing PCR cycle number close to the Ct value. Throat swabs from healthy volunteers were extracted with Qiagen DNeasy (D) or MO BIO PowerSoil (M). DNA extracts were subjected to 16S gene TaqMan qPCR and subsequent PCR using a cycle number of 20, 25, or 30 based on individual sample's qPCR Ct value. PCR amplicons were quantified by PicoGreen dsDNA assay. DNA concentrations for DNA extracts ( blue markers and axis ) and PCR amplicons ( red markers and axis ) are shown in 16S gene copies/μl in the same scale. DNA sample name, 08S1M as an example, depicts the subject number (08), swab number (S1), and extraction method (M).
    Figure Legend Snippet: Normalization of PCR amplification by choosing PCR cycle number close to the Ct value. Throat swabs from healthy volunteers were extracted with Qiagen DNeasy (D) or MO BIO PowerSoil (M). DNA extracts were subjected to 16S gene TaqMan qPCR and subsequent PCR using a cycle number of 20, 25, or 30 based on individual sample's qPCR Ct value. PCR amplicons were quantified by PicoGreen dsDNA assay. DNA concentrations for DNA extracts ( blue markers and axis ) and PCR amplicons ( red markers and axis ) are shown in 16S gene copies/μl in the same scale. DNA sample name, 08S1M as an example, depicts the subject number (08), swab number (S1), and extraction method (M).

    Techniques Used: Polymerase Chain Reaction, Amplification, Real-time Polymerase Chain Reaction, Picogreen Assay

    Storage temperature comparisons for Qiagen DNeasy and MO BIO PowerSoil DNA extraction methods for the mock bacterial community HM-280. Identical samples from the mock community were stored at four different temperatures and extracted after 4 weeks using the two methods, Qiagen DNeasy and MO BIO PowerSoil. Microbial profiles at the genus level were estimated using QIIME. Overall, samples were well preserved at lower temperatures for both methods, whereas significant differences were observed at 37°C.
    Figure Legend Snippet: Storage temperature comparisons for Qiagen DNeasy and MO BIO PowerSoil DNA extraction methods for the mock bacterial community HM-280. Identical samples from the mock community were stored at four different temperatures and extracted after 4 weeks using the two methods, Qiagen DNeasy and MO BIO PowerSoil. Microbial profiles at the genus level were estimated using QIIME. Overall, samples were well preserved at lower temperatures for both methods, whereas significant differences were observed at 37°C.

    Techniques Used: DNA Extraction

    17) Product Images from "16S rRNA gene pyrosequencing of reference and clinical samples and investigation of the temperature stability of microbiome profiles"

    Article Title: 16S rRNA gene pyrosequencing of reference and clinical samples and investigation of the temperature stability of microbiome profiles

    Journal: Microbiome

    doi: 10.1186/2049-2618-2-31

    Normalization of PCR amplification by choosing PCR cycle number close to the Ct value. Throat swabs from healthy volunteers were extracted with Qiagen DNeasy (D) or MO BIO PowerSoil (M). DNA extracts were subjected to 16S gene TaqMan qPCR and subsequent PCR using a cycle number of 20, 25, or 30 based on individual sample's qPCR Ct value. PCR amplicons were quantified by PicoGreen dsDNA assay. DNA concentrations for DNA extracts ( blue markers and axis ) and PCR amplicons ( red markers and axis ) are shown in 16S gene copies/μl in the same scale. DNA sample name, 08S1M as an example, depicts the subject number (08), swab number (S1), and extraction method (M).
    Figure Legend Snippet: Normalization of PCR amplification by choosing PCR cycle number close to the Ct value. Throat swabs from healthy volunteers were extracted with Qiagen DNeasy (D) or MO BIO PowerSoil (M). DNA extracts were subjected to 16S gene TaqMan qPCR and subsequent PCR using a cycle number of 20, 25, or 30 based on individual sample's qPCR Ct value. PCR amplicons were quantified by PicoGreen dsDNA assay. DNA concentrations for DNA extracts ( blue markers and axis ) and PCR amplicons ( red markers and axis ) are shown in 16S gene copies/μl in the same scale. DNA sample name, 08S1M as an example, depicts the subject number (08), swab number (S1), and extraction method (M).

    Techniques Used: Polymerase Chain Reaction, Amplification, Real-time Polymerase Chain Reaction, Picogreen Assay

    Storage temperature comparisons for Qiagen DNeasy and MO BIO PowerSoil DNA extraction methods for the mock bacterial community HM-280. Identical samples from the mock community were stored at four different temperatures and extracted after 4 weeks using the two methods, Qiagen DNeasy and MO BIO PowerSoil. Microbial profiles at the genus level were estimated using QIIME. Overall, samples were well preserved at lower temperatures for both methods, whereas significant differences were observed at 37°C.
    Figure Legend Snippet: Storage temperature comparisons for Qiagen DNeasy and MO BIO PowerSoil DNA extraction methods for the mock bacterial community HM-280. Identical samples from the mock community were stored at four different temperatures and extracted after 4 weeks using the two methods, Qiagen DNeasy and MO BIO PowerSoil. Microbial profiles at the genus level were estimated using QIIME. Overall, samples were well preserved at lower temperatures for both methods, whereas significant differences were observed at 37°C.

    Techniques Used: DNA Extraction

    18) Product Images from "Impact of Hydrodynamic Injection and phiC31 Integrase on Tumor Latency in a Mouse Model of MYC-Induced Hepatocellular Carcinoma"

    Article Title: Impact of Hydrodynamic Injection and phiC31 Integrase on Tumor Latency in a Mouse Model of MYC-Induced Hepatocellular Carcinoma

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0011367

    Luciferase activity and PCR analysis of tumors from mice in the pCSI/pLiLucB group provide no evidence of ϕC31 integrase activity. ( a ) Protein extracts were prepared and the luciferase activity was measured in absolute counts per second (CPS). Controls included HeLa cells given FuGene 6 alone [HeLa (-)] or the CMV-luciferase plasmid pNBL2 via FuGene 6 [HeLa (+)], the normal-appearing part of the tumor-ridden liver taken from either a saline-injected mouse [Liver (-)] and pCSI/pLiLucB-injected mouse [Liver (+)]. Eight tumor samples (Tumor 1 through 8) and one metastasis (Met 1) that were obtained from four animals were also analyzed. The error bars give standard error of the mean for four replicates of each sample. ( b ) PCR analysis to detect the pLiLucB plasmid by amplification of the luciferase transgene. Plasmid DNA (20 ng pLiLucB) and no DNA controls show specific amplification of luciferase only in the reaction containing plasmid. One mouse each from the saline-only and pCSmI/pLiLucB groups was analyzed for transgene presence in normal-appearing (N) and tumor (T) tissues (none found). Three mice in the pCSI/pLiLucB group were analyzed for transgene presence in normal-appearing (N) and tumor (T) tissues. Luciferase could be detected in 2/3 normal-appearing liver samples and none of the tumors. ( c ) PCR analysis for integration at the mpsL1 pseudo attP site was done on 18 tumors (lanes 5 through 22) and one metastasis (lane 23) taken from nine mice given pCSI/pLiLucB by hydrodynamic injection. Controls included no DNA (1 st round, lane 1 and 2 nd round, lane 25), and a DNeasy performed on no tissue (lane 2) to show no contamination from the DNA isolation procedure. Normal-appearing liver from a mouse in the pCSI/pLiLucB group (lane 3) served as the positive control. DNA isolated from a tumor in the saline-only group served as the negative control (lane 4). PCR for the GAPDH gene showed that sufficient DNA was added to all reactions. Seven tumors and one metastasis were subjected to the analysis in both a and c . Six tumors were analyzed by all assays ( a , b and c ).
    Figure Legend Snippet: Luciferase activity and PCR analysis of tumors from mice in the pCSI/pLiLucB group provide no evidence of ϕC31 integrase activity. ( a ) Protein extracts were prepared and the luciferase activity was measured in absolute counts per second (CPS). Controls included HeLa cells given FuGene 6 alone [HeLa (-)] or the CMV-luciferase plasmid pNBL2 via FuGene 6 [HeLa (+)], the normal-appearing part of the tumor-ridden liver taken from either a saline-injected mouse [Liver (-)] and pCSI/pLiLucB-injected mouse [Liver (+)]. Eight tumor samples (Tumor 1 through 8) and one metastasis (Met 1) that were obtained from four animals were also analyzed. The error bars give standard error of the mean for four replicates of each sample. ( b ) PCR analysis to detect the pLiLucB plasmid by amplification of the luciferase transgene. Plasmid DNA (20 ng pLiLucB) and no DNA controls show specific amplification of luciferase only in the reaction containing plasmid. One mouse each from the saline-only and pCSmI/pLiLucB groups was analyzed for transgene presence in normal-appearing (N) and tumor (T) tissues (none found). Three mice in the pCSI/pLiLucB group were analyzed for transgene presence in normal-appearing (N) and tumor (T) tissues. Luciferase could be detected in 2/3 normal-appearing liver samples and none of the tumors. ( c ) PCR analysis for integration at the mpsL1 pseudo attP site was done on 18 tumors (lanes 5 through 22) and one metastasis (lane 23) taken from nine mice given pCSI/pLiLucB by hydrodynamic injection. Controls included no DNA (1 st round, lane 1 and 2 nd round, lane 25), and a DNeasy performed on no tissue (lane 2) to show no contamination from the DNA isolation procedure. Normal-appearing liver from a mouse in the pCSI/pLiLucB group (lane 3) served as the positive control. DNA isolated from a tumor in the saline-only group served as the negative control (lane 4). PCR for the GAPDH gene showed that sufficient DNA was added to all reactions. Seven tumors and one metastasis were subjected to the analysis in both a and c . Six tumors were analyzed by all assays ( a , b and c ).

    Techniques Used: Luciferase, Activity Assay, Polymerase Chain Reaction, Mouse Assay, Plasmid Preparation, Injection, Amplification, DNA Extraction, Positive Control, Isolation, Negative Control

    19) Product Images from "Optimizing an eDNA protocol for monitoring endangered Chinook Salmon in the San Francisco Estuary: balancing sensitivity, cost and time"

    Article Title: Optimizing an eDNA protocol for monitoring endangered Chinook Salmon in the San Francisco Estuary: balancing sensitivity, cost and time

    Journal: bioRxiv

    doi: 10.1101/871368

    Modelled distribution of percentage yield for each extraction protocol. The width of each of the curves shows the variability in modelled yield. The peak of the distribution is the mean yield per extraction type. QIagen DNeasy yields the best yield with little overlap with other methods. Meanwhile magnetic beads and NaOH have shown similar distributions with significant overlap, while both dipstick methods underperform the other methods. It is important to note that this plot does not take into account PCR inhibitor carryover, which might vary significantly between methods.
    Figure Legend Snippet: Modelled distribution of percentage yield for each extraction protocol. The width of each of the curves shows the variability in modelled yield. The peak of the distribution is the mean yield per extraction type. QIagen DNeasy yields the best yield with little overlap with other methods. Meanwhile magnetic beads and NaOH have shown similar distributions with significant overlap, while both dipstick methods underperform the other methods. It is important to note that this plot does not take into account PCR inhibitor carryover, which might vary significantly between methods.

    Techniques Used: Magnetic Beads, Polymerase Chain Reaction

    20) Product Images from "Design of a titering assay for lentiviral vectors utilizing direct extraction of DNA from transduced cells in microtiter plates"

    Article Title: Design of a titering assay for lentiviral vectors utilizing direct extraction of DNA from transduced cells in microtiter plates

    Journal: Molecular Therapy. Methods & Clinical Development

    doi: 10.1038/mtm.2016.5

    Comparison of vector transduction titers measured following in situ versus spin column (DNeasy) DNA extraction. 293T-MLV-DCSIGN assay cells were transduced with equivalent amounts of integration-competent or -deficient vector particles (as measured by vector genomes). Following an overnight incubation, DNA was isolated, and samples were analyzed by qPCR to detect vector sequence. ( a ) Extraction performed using in situ method. Each bar represents the mean ± SD of nine transductions performed in parallel ( n = 3 qPCR replicates/transduction). ( b ) Extraction performed using DNeasy column (5 × 10 4 cells loaded per column). Each bar represents the mean ± SD of qPCR triplicates for a single transduction; two transductions were performed in parallel for each vector tested (replicates 1, 2). Particle-to-infectivity (P:I) ratios were calculated by dividing the physical particle titer (measured by RNA genome content per vector) by the measured transduction titer. Results in both panels are representative of two independent experiments. TU, transduction units.
    Figure Legend Snippet: Comparison of vector transduction titers measured following in situ versus spin column (DNeasy) DNA extraction. 293T-MLV-DCSIGN assay cells were transduced with equivalent amounts of integration-competent or -deficient vector particles (as measured by vector genomes). Following an overnight incubation, DNA was isolated, and samples were analyzed by qPCR to detect vector sequence. ( a ) Extraction performed using in situ method. Each bar represents the mean ± SD of nine transductions performed in parallel ( n = 3 qPCR replicates/transduction). ( b ) Extraction performed using DNeasy column (5 × 10 4 cells loaded per column). Each bar represents the mean ± SD of qPCR triplicates for a single transduction; two transductions were performed in parallel for each vector tested (replicates 1, 2). Particle-to-infectivity (P:I) ratios were calculated by dividing the physical particle titer (measured by RNA genome content per vector) by the measured transduction titer. Results in both panels are representative of two independent experiments. TU, transduction units.

    Techniques Used: Plasmid Preparation, Transduction, In Situ, DNA Extraction, Incubation, Isolation, Real-time Polymerase Chain Reaction, Sequencing, Infection

    21) Product Images from "Development of Loop-Mediated Isothermal Amplification Targeting 18S Ribosomal DNA for Rapid Detection of Azumiobodo hoyamushi (Kinetoplastea)"

    Article Title: Development of Loop-Mediated Isothermal Amplification Targeting 18S Ribosomal DNA for Rapid Detection of Azumiobodo hoyamushi (Kinetoplastea)

    Journal: The Korean Journal of Parasitology

    doi: 10.3347/kjp.2014.52.3.305

    Detection limit of Azumiobodo hoyamushi 18S rDNA LAMP assays (A). LAMP assays were performed using serial dilutions of A. hoyamushi genomic DNA (1 ng to 1 fg per reaction). Distilled water was used as a negative control. LAMP products were visualized by gel electrophoresis (B) and using Loopamp® fluorescent detection reagent (FD) (C). (B, C) Lane M, 100-bp DNA marker; lane 1, 1 ng; lane 2, 100 pg; lane 3, 10 pg; lane 4, 1 pg; lane 5, 100 fg; lane 6, 10 fg; lane 7, 1 fg of A. hoyamushi genomic DNA; lane 8, distilled water; and lane 9, LAMP product after Mbo I digestion. (D-E) A. hoyamushi at a density of 1×10 3 parasites/µl was serially diluted and tested (D) using the LAMP assay (D) and by PCR (E) using F3 and B3 primers. Lane M, 100-bp DNA marker; lane 1, 1,000; lane 2, 100; lane 3, 10; lane 4, 1; lane 5, 0.1; lane 6, 0.01 of parasites per reaction; lane 7, distilled water. A. hoyamushi genomic DNA was prepared using DNeasy tissue kits (Qiagen) from in vitro cultured A. hoyamushi species [ 9 ] which were kindly provided by Dr. Kyung Il Park (Kunsan National University, Gunsan, Korea).
    Figure Legend Snippet: Detection limit of Azumiobodo hoyamushi 18S rDNA LAMP assays (A). LAMP assays were performed using serial dilutions of A. hoyamushi genomic DNA (1 ng to 1 fg per reaction). Distilled water was used as a negative control. LAMP products were visualized by gel electrophoresis (B) and using Loopamp® fluorescent detection reagent (FD) (C). (B, C) Lane M, 100-bp DNA marker; lane 1, 1 ng; lane 2, 100 pg; lane 3, 10 pg; lane 4, 1 pg; lane 5, 100 fg; lane 6, 10 fg; lane 7, 1 fg of A. hoyamushi genomic DNA; lane 8, distilled water; and lane 9, LAMP product after Mbo I digestion. (D-E) A. hoyamushi at a density of 1×10 3 parasites/µl was serially diluted and tested (D) using the LAMP assay (D) and by PCR (E) using F3 and B3 primers. Lane M, 100-bp DNA marker; lane 1, 1,000; lane 2, 100; lane 3, 10; lane 4, 1; lane 5, 0.1; lane 6, 0.01 of parasites per reaction; lane 7, distilled water. A. hoyamushi genomic DNA was prepared using DNeasy tissue kits (Qiagen) from in vitro cultured A. hoyamushi species [ 9 ] which were kindly provided by Dr. Kyung Il Park (Kunsan National University, Gunsan, Korea).

    Techniques Used: Negative Control, Nucleic Acid Electrophoresis, Marker, Lamp Assay, Polymerase Chain Reaction, In Vitro, Cell Culture

    22) Product Images from "Conventional and PCR Detection of Aphelenchoides fragariae in Diverse Ornamental Host Plant Species"

    Article Title: Conventional and PCR Detection of Aphelenchoides fragariae in Diverse Ornamental Host Plant Species

    Journal:

    doi:

    Comparison of DNA extraction methods for detection of Aphelenchoides fragariae in naturally infected Asplenium nidus (Bird's nest fern) plant tissue using species-specific PCR primers. Lane 1: healthy A. nidus extracted with Qiagen Dneasy Plant Mini Kit;
    Figure Legend Snippet: Comparison of DNA extraction methods for detection of Aphelenchoides fragariae in naturally infected Asplenium nidus (Bird's nest fern) plant tissue using species-specific PCR primers. Lane 1: healthy A. nidus extracted with Qiagen Dneasy Plant Mini Kit;

    Techniques Used: DNA Extraction, Infection, Polymerase Chain Reaction

    23) Product Images from "Loop-mediated isothermal amplification (LAMP) assay—A rapid detection tool for identifying red fox (Vulpes vulpes) DNA in the carcasses of harbour porpoises (Phocoena phocoena)"

    Article Title: Loop-mediated isothermal amplification (LAMP) assay—A rapid detection tool for identifying red fox (Vulpes vulpes) DNA in the carcasses of harbour porpoises (Phocoena phocoena)

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0184349

    Application of the LAMP assay on a stranded harbour porpoise. Gel electrophoresis of all LAMP products of the stranded harbour porpoise. Directly tested MSwab ™ samples (without DNA isolation) and DNA isolation of the MSwab ™ medium using DNeasy ® blood and tissue kit.
    Figure Legend Snippet: Application of the LAMP assay on a stranded harbour porpoise. Gel electrophoresis of all LAMP products of the stranded harbour porpoise. Directly tested MSwab ™ samples (without DNA isolation) and DNA isolation of the MSwab ™ medium using DNeasy ® blood and tissue kit.

    Techniques Used: Lamp Assay, Nucleic Acid Electrophoresis, DNA Extraction

    24) Product Images from "Isolation and preservation of schistosome eggs and larvae in RNAlater® facilitates genetic profiling of individuals"

    Article Title: Isolation and preservation of schistosome eggs and larvae in RNAlater® facilitates genetic profiling of individuals

    Journal: Parasites & Vectors

    doi: 10.1186/1756-3305-2-50

    Schematic of the RNA later ® preservation and gDNA extraction of individual schistosome larval stages and eggs . ATL and AL are lysis buffers supplied in the Qiagen DNeasy Blood and Tissue Kit. *These were the longest times and highest temperatures tested but it is expected that the samples can be preserved for much longer.
    Figure Legend Snippet: Schematic of the RNA later ® preservation and gDNA extraction of individual schistosome larval stages and eggs . ATL and AL are lysis buffers supplied in the Qiagen DNeasy Blood and Tissue Kit. *These were the longest times and highest temperatures tested but it is expected that the samples can be preserved for much longer.

    Techniques Used: Preserving, Lysis

    25) Product Images from "Species‐level biodiversity assessment using marine environmental DNA metabarcoding requires protocol optimization and standardization, et al. Species‐level biodiversity assessment using marine environmental DNA metabarcoding requires protocol optimization and standardization"

    Article Title: Species‐level biodiversity assessment using marine environmental DNA metabarcoding requires protocol optimization and standardization, et al. Species‐level biodiversity assessment using marine environmental DNA metabarcoding requires protocol optimization and standardization

    Journal: Ecology and Evolution

    doi: 10.1002/ece3.4843

    DNA yield from seven extraction protocols (DNeasy: Qiagen DNeasy Blood Tissue Kit; MO BIO PW: MO BIO PowerWater DNA Isolation Kit; MO BIO PMS: MO BIO PowerMax Soil DNA Isolation Kit; Dnature: Presto™ Mini gDNA Bacteria Kit; PCI: phenol‐chloroform‐isoamyl extraction procedure; Silica: Silica extraction procedure; and Magnetic beads: Magnetic beads extraction procedure) obtained from 1,000 ml technical replicates filtered through 1.2‐µm cellulose‐nitrate filters. Error bars show 95% confidence intervals. Letters indicate significant differences among groups based on Tukey–Kramer's test (α = 0.05)
    Figure Legend Snippet: DNA yield from seven extraction protocols (DNeasy: Qiagen DNeasy Blood Tissue Kit; MO BIO PW: MO BIO PowerWater DNA Isolation Kit; MO BIO PMS: MO BIO PowerMax Soil DNA Isolation Kit; Dnature: Presto™ Mini gDNA Bacteria Kit; PCI: phenol‐chloroform‐isoamyl extraction procedure; Silica: Silica extraction procedure; and Magnetic beads: Magnetic beads extraction procedure) obtained from 1,000 ml technical replicates filtered through 1.2‐µm cellulose‐nitrate filters. Error bars show 95% confidence intervals. Letters indicate significant differences among groups based on Tukey–Kramer's test (α = 0.05)

    Techniques Used: DNA Extraction, Magnetic Beads

    The average (a) OTU and (b) taxon richness obtained per replicate for each of the four assays between the optimal (blue; 1.2‐µm cellulose‐nitrate filter and Qiagen's DNeasy Blood Tissue Kit) and low‐performance (gold; 1.2‐µm polycarbonate filter and MO BIO's PowerMax Soil) protocol. Error bars show 95% confidence intervals. T test significance is depicted by: *** p
    Figure Legend Snippet: The average (a) OTU and (b) taxon richness obtained per replicate for each of the four assays between the optimal (blue; 1.2‐µm cellulose‐nitrate filter and Qiagen's DNeasy Blood Tissue Kit) and low‐performance (gold; 1.2‐µm polycarbonate filter and MO BIO's PowerMax Soil) protocol. Error bars show 95% confidence intervals. T test significance is depicted by: *** p

    Techniques Used:

    Observed taxa in each of the four assays. Filled and unfilled rectangles indicate taxon presence or absence. Taxa show a pair of rectangles per assay, representing the low‐performance (PCPMS; 1.2‐µm polycarbonate filter and MO BIO's PowerMax Soil) and the optimal (CNQ; 1.2‐µm cellulose‐nitrate filter and Qiagen's DNeasy Blood Tissue Kit) treatment. Rectangular presence indicates the amplification range of each assay
    Figure Legend Snippet: Observed taxa in each of the four assays. Filled and unfilled rectangles indicate taxon presence or absence. Taxa show a pair of rectangles per assay, representing the low‐performance (PCPMS; 1.2‐µm polycarbonate filter and MO BIO's PowerMax Soil) and the optimal (CNQ; 1.2‐µm cellulose‐nitrate filter and Qiagen's DNeasy Blood Tissue Kit) treatment. Rectangular presence indicates the amplification range of each assay

    Techniques Used: Amplification

    26) Product Images from "Comparison of DNA extraction methods for non-marine molluscs: is modified CTAB DNA extraction method more efficient than DNA extraction kits?"

    Article Title: Comparison of DNA extraction methods for non-marine molluscs: is modified CTAB DNA extraction method more efficient than DNA extraction kits?

    Journal: 3 Biotech

    doi: 10.1007/s13205-020-2051-7

    Chromatogram depicting 16S gene sequence quality in Qiagen ® DNeasy Blood and Tissue Kit extracted DNA
    Figure Legend Snippet: Chromatogram depicting 16S gene sequence quality in Qiagen ® DNeasy Blood and Tissue Kit extracted DNA

    Techniques Used: Sequencing

    27) Product Images from "Comparing Two Common DNA Extraction Kits for the Characterization of Symbiotic Microbial Communities from Ascidian Tissue"

    Article Title: Comparing Two Common DNA Extraction Kits for the Characterization of Symbiotic Microbial Communities from Ascidian Tissue

    Journal: Microbes and Environments

    doi: 10.1264/jsme2.ME18031

    Non-metric multi-dimensional scaling plot based on the Bray-Curtis similarity of microbial communities obtained using DNeasy (black triangles) and PowerSoil (gray circles) kits. Circles encompass samples clustering at > 30% similarity and correspond to replicates of Clavelina oblonga (dashed line) and Polyandrocarpa anguinea (solid line), indicating a high degree of host-specificity and low technical variation across DNA extraction methods.
    Figure Legend Snippet: Non-metric multi-dimensional scaling plot based on the Bray-Curtis similarity of microbial communities obtained using DNeasy (black triangles) and PowerSoil (gray circles) kits. Circles encompass samples clustering at > 30% similarity and correspond to replicates of Clavelina oblonga (dashed line) and Polyandrocarpa anguinea (solid line), indicating a high degree of host-specificity and low technical variation across DNA extraction methods.

    Techniques Used: DNA Extraction

    Microbial community composition averaged by source and kit (a), and for each replicate sample within each source: Clavelina oblonga , DNeasy (b), C. oblonga , PowerSoil (c), Polyandrocarpa anguinea , DNeasy (d), and P. anguinea , PowerSoil (e). Phylum-level classifications are shown, except for Proteobacteria , which were divided into four classes: Alpha -, Gamma -, Delta -, and Epsilonproteobacteria . Each library represents 20,007 sequence reads.
    Figure Legend Snippet: Microbial community composition averaged by source and kit (a), and for each replicate sample within each source: Clavelina oblonga , DNeasy (b), C. oblonga , PowerSoil (c), Polyandrocarpa anguinea , DNeasy (d), and P. anguinea , PowerSoil (e). Phylum-level classifications are shown, except for Proteobacteria , which were divided into four classes: Alpha -, Gamma -, Delta -, and Epsilonproteobacteria . Each library represents 20,007 sequence reads.

    Techniques Used: Sequencing

    28) Product Images from "Demonstration of the Use of Environmental DNA for the Non-Invasive Genotyping of a Bivalve Mollusk, the European Flat Oyster (Ostrea edulis)"

    Article Title: Demonstration of the Use of Environmental DNA for the Non-Invasive Genotyping of a Bivalve Mollusk, the European Flat Oyster (Ostrea edulis)

    Journal: Frontiers in Genetics

    doi: 10.3389/fgene.2019.01159

    Diagram (top) detailing dilutions of DNA template from different DNA extraction techniques used for SNP genotyping of environmental DNA for Ostrea edulis . The two DNA extraction methods, the Qiagen DNeasy Blood and Tissue Kit and the crude Chelex extraction, are shown at the top. DNA samples are subject to dilution step before and after the Specific Target Amplification PCR (STA PCR), here shown as a blue bar. All dilutions were with PCR grade water and samples that were not diluted are labelled ‘neat’. A scatter plot (bottom) shows the percentage of correct environmental DNA derived genotype calls in comparison to the tissue extractions for the eight dilutions. Each point represents the total result derived from a random sample of three replicates per individual oyster genotype. The black line indicates the average percentage correct eDNA genotypes across the 100 random samples. The red line indicates the average percentage correct eDNA genotypes across the 100 samples if only a single replicate is used for each individual oyster genotype.
    Figure Legend Snippet: Diagram (top) detailing dilutions of DNA template from different DNA extraction techniques used for SNP genotyping of environmental DNA for Ostrea edulis . The two DNA extraction methods, the Qiagen DNeasy Blood and Tissue Kit and the crude Chelex extraction, are shown at the top. DNA samples are subject to dilution step before and after the Specific Target Amplification PCR (STA PCR), here shown as a blue bar. All dilutions were with PCR grade water and samples that were not diluted are labelled ‘neat’. A scatter plot (bottom) shows the percentage of correct environmental DNA derived genotype calls in comparison to the tissue extractions for the eight dilutions. Each point represents the total result derived from a random sample of three replicates per individual oyster genotype. The black line indicates the average percentage correct eDNA genotypes across the 100 random samples. The red line indicates the average percentage correct eDNA genotypes across the 100 samples if only a single replicate is used for each individual oyster genotype.

    Techniques Used: DNA Extraction, Amplification, Polymerase Chain Reaction, Derivative Assay

    29) Product Images from "Comparison of four DNA extraction and three preservation protocols for the molecular detection and quantification of soil-transmitted helminths in stool"

    Article Title: Comparison of four DNA extraction and three preservation protocols for the molecular detection and quantification of soil-transmitted helminths in stool

    Journal: PLoS Neglected Tropical Diseases

    doi: 10.1371/journal.pntd.0007778

    The sensitivity and DNA concentration across four DNA extraction protocols for Trichuris and Necator . The left panel represents the sensitivity (sen) and geometric mean of DNA concentration expressed as genome equivalents per ml of DNA extract (mean; GE/ml) for 20 Necator americanus samples preserved in 96% ethanol and extracted by four DNA extraction protocols. The DNA extraction protocols include the QIAamp DNA Stool Mini kit without (SK) and with bead beating (SK + BB), DNeasy Blood Tissue kit without (TK) and without bead beating (TK + BB). The right panel represents the same parameters across 36 Trichuris trichiura samples preserved in 96% ethanol. Each line represents a sample.
    Figure Legend Snippet: The sensitivity and DNA concentration across four DNA extraction protocols for Trichuris and Necator . The left panel represents the sensitivity (sen) and geometric mean of DNA concentration expressed as genome equivalents per ml of DNA extract (mean; GE/ml) for 20 Necator americanus samples preserved in 96% ethanol and extracted by four DNA extraction protocols. The DNA extraction protocols include the QIAamp DNA Stool Mini kit without (SK) and with bead beating (SK + BB), DNeasy Blood Tissue kit without (TK) and without bead beating (TK + BB). The right panel represents the same parameters across 36 Trichuris trichiura samples preserved in 96% ethanol. Each line represents a sample.

    Techniques Used: Concentration Assay, DNA Extraction

    30) Product Images from "Comparison of four DNA extraction and three preservation protocols for the molecular detection and quantification of soil-transmitted helminths in stool"

    Article Title: Comparison of four DNA extraction and three preservation protocols for the molecular detection and quantification of soil-transmitted helminths in stool

    Journal: PLoS Neglected Tropical Diseases

    doi: 10.1371/journal.pntd.0007778

    The sensitivity and DNA concentration across four DNA extraction protocols for Trichuris and Necator . The left panel represents the sensitivity (sen) and geometric mean of DNA concentration expressed as genome equivalents per ml of DNA extract (mean; GE/ml) for 20 Necator americanus samples preserved in 96% ethanol and extracted by four DNA extraction protocols. The DNA extraction protocols include the QIAamp DNA Stool Mini kit without (SK) and with bead beating (SK + BB), DNeasy Blood Tissue kit without (TK) and without bead beating (TK + BB). The right panel represents the same parameters across 36 Trichuris trichiura samples preserved in 96% ethanol. Each line represents a sample.
    Figure Legend Snippet: The sensitivity and DNA concentration across four DNA extraction protocols for Trichuris and Necator . The left panel represents the sensitivity (sen) and geometric mean of DNA concentration expressed as genome equivalents per ml of DNA extract (mean; GE/ml) for 20 Necator americanus samples preserved in 96% ethanol and extracted by four DNA extraction protocols. The DNA extraction protocols include the QIAamp DNA Stool Mini kit without (SK) and with bead beating (SK + BB), DNeasy Blood Tissue kit without (TK) and without bead beating (TK + BB). The right panel represents the same parameters across 36 Trichuris trichiura samples preserved in 96% ethanol. Each line represents a sample.

    Techniques Used: Concentration Assay, DNA Extraction

    31) Product Images from "Evaluation of rapid extraction and isothermal amplification techniques for the detection of Leishmania donovani DNA from skin lesions of suspected cases at the point of need in Sri Lanka"

    Article Title: Evaluation of rapid extraction and isothermal amplification techniques for the detection of Leishmania donovani DNA from skin lesions of suspected cases at the point of need in Sri Lanka

    Journal: Parasites & Vectors

    doi: 10.1186/s13071-018-3238-1

    Comparison between the sample types and test methods. The slit skin smears were examined with microscopy. The skin scrapping and aspirate were tested at the point of need with SpeedXtract (SE) and recombinase polymerase amplification (RPA) assay. The punch biopsies were screened with SE and RPA as well as DNeasy blood and tissue kit (mini) and polymerase chain reaction (PCR). Abbreviations : ID, sample identification code; POS, Leishmania donovani was detected; NEG, negative test results
    Figure Legend Snippet: Comparison between the sample types and test methods. The slit skin smears were examined with microscopy. The skin scrapping and aspirate were tested at the point of need with SpeedXtract (SE) and recombinase polymerase amplification (RPA) assay. The punch biopsies were screened with SE and RPA as well as DNeasy blood and tissue kit (mini) and polymerase chain reaction (PCR). Abbreviations : ID, sample identification code; POS, Leishmania donovani was detected; NEG, negative test results

    Techniques Used: Microscopy, Recombinase Polymerase Amplification, Polymerase Chain Reaction

    32) Product Images from "A vicious loop of fatty acid-binding protein 4 and DNA methyltransferase 1 promotes acute myeloid leukemia and acts as a therapeutic target"

    Article Title: A vicious loop of fatty acid-binding protein 4 and DNA methyltransferase 1 promotes acute myeloid leukemia and acts as a therapeutic target

    Journal: Leukemia

    doi: 10.1038/leu.2017.307

    Pharmacological inhibition of FABP4 induces DNA hypomethylation and restores the epigenetically silenced p15 INK4N . ( a and b ) Western blotting of C1498, MV4-11 or Kasumi-1 cells exposed to BMS. ( c ) C1498, MV4-11 or Kasumi-1 cells were treated with BMS and the genomic DNA was subjected to Dotblotting. Left, representative images of dots; right, graph shows quantification of dot intensity. ( d ) qPCR of C1498, MV4-11 or Kasumi-1 cells treated with BMS. ( e ) Bisulfite sequencing of the p15 INK4B promoter in MV4-11 or Kasumi-1 cells treated with BMS. Results of 10 clones are presented. Vertical bars indicate CpG locations, arrows indicate the bisulfite sequencing region, open circles show unmethylated CpG, and solid circles show methylated CpGs. ( f ) qPCR of human patient (n = 8) or mouse (n = 3) AML primary cells treated with BMS. The experiments were performed three times independently and data are shown as mean values ± SD, * P
    Figure Legend Snippet: Pharmacological inhibition of FABP4 induces DNA hypomethylation and restores the epigenetically silenced p15 INK4N . ( a and b ) Western blotting of C1498, MV4-11 or Kasumi-1 cells exposed to BMS. ( c ) C1498, MV4-11 or Kasumi-1 cells were treated with BMS and the genomic DNA was subjected to Dotblotting. Left, representative images of dots; right, graph shows quantification of dot intensity. ( d ) qPCR of C1498, MV4-11 or Kasumi-1 cells treated with BMS. ( e ) Bisulfite sequencing of the p15 INK4B promoter in MV4-11 or Kasumi-1 cells treated with BMS. Results of 10 clones are presented. Vertical bars indicate CpG locations, arrows indicate the bisulfite sequencing region, open circles show unmethylated CpG, and solid circles show methylated CpGs. ( f ) qPCR of human patient (n = 8) or mouse (n = 3) AML primary cells treated with BMS. The experiments were performed three times independently and data are shown as mean values ± SD, * P

    Techniques Used: Inhibition, Western Blot, Real-time Polymerase Chain Reaction, Methylation Sequencing, Clone Assay, Methylation

    33) Product Images from "Development of real-time PCR for detection of Mycoplasma hominis"

    Article Title: Development of real-time PCR for detection of Mycoplasma hominis

    Journal: BMC Microbiology

    doi: 10.1186/1471-2180-4-35

    DNeasy treated samples. (a) Two LightCycler PCR runs, first one with standard dilution series of human Hep2 DNA before (flat negative curves) and after DNeasy (indicated with squares and triangles), showed on the left and second with DNA free water before (marked with squares) and after DNeasy (triangles) on the right. (b) Melting curve analysis of DNeasy treated H 2 O (triangles), Hep2 DNA (squares) and clinical sample.
    Figure Legend Snippet: DNeasy treated samples. (a) Two LightCycler PCR runs, first one with standard dilution series of human Hep2 DNA before (flat negative curves) and after DNeasy (indicated with squares and triangles), showed on the left and second with DNA free water before (marked with squares) and after DNeasy (triangles) on the right. (b) Melting curve analysis of DNeasy treated H 2 O (triangles), Hep2 DNA (squares) and clinical sample.

    Techniques Used: Polymerase Chain Reaction

    34) Product Images from "Tissue Fractionation by Hydrostatic Pressure Cycling Technology: The Unified Sample Preparation Technique for Systems Biology Studies"

    Article Title: Tissue Fractionation by Hydrostatic Pressure Cycling Technology: The Unified Sample Preparation Technique for Systems Biology Studies

    Journal: Journal of Biomolecular Techniques : JBT

    doi:

    A: DNA and protein recovery from the cell culture. one aliquot of cells was processed with PCT and ProteoSolve-SB (P). DNA was extracted from the solid phase using the DNeasy kit. The control aliquot of cells was processed directly with the qiagen DNeasy
    Figure Legend Snippet: A: DNA and protein recovery from the cell culture. one aliquot of cells was processed with PCT and ProteoSolve-SB (P). DNA was extracted from the solid phase using the DNeasy kit. The control aliquot of cells was processed directly with the qiagen DNeasy

    Techniques Used: Cell Culture

    35) Product Images from "Dissemination of Orientia tsutsugamushi, a Causative Agent of Scrub Typhus, and Immunological Responses in the Humanized DRAGA Mouse"

    Article Title: Dissemination of Orientia tsutsugamushi, a Causative Agent of Scrub Typhus, and Immunological Responses in the Humanized DRAGA Mouse

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2018.00816

    Live Orientia tsutsugamushi disseminates into organs of infected humanized DRAGA mice. (A) DNA was extracted from various organs of humanized DRAGA mice 2 weeks post infection at 6 × 10 4 mLD 50 and O. tsutsugamushi was quantified by quantitative PCR (qPCR). (B) Immunofluorescence staining showing O. tsutsugamushi (red) in lung tissue frozen sections from humanized DRAGA mice 3 weeks post infection at 6 × 10 2 mLD 50 (cell nuclei were stained blue with DAPI, magnification, 400×). (C) Tissue homogenates were prepared from lung tissue and used to inoculate L929 cells. Immunofluorescence staining was performed to detect the presence of O. tsutsugamushi (magnification, 400×) at day 7 and day 14 post inoculation. (D) qPCR was used to quantify number of Orientia in L929 cells collected in (C) .
    Figure Legend Snippet: Live Orientia tsutsugamushi disseminates into organs of infected humanized DRAGA mice. (A) DNA was extracted from various organs of humanized DRAGA mice 2 weeks post infection at 6 × 10 4 mLD 50 and O. tsutsugamushi was quantified by quantitative PCR (qPCR). (B) Immunofluorescence staining showing O. tsutsugamushi (red) in lung tissue frozen sections from humanized DRAGA mice 3 weeks post infection at 6 × 10 2 mLD 50 (cell nuclei were stained blue with DAPI, magnification, 400×). (C) Tissue homogenates were prepared from lung tissue and used to inoculate L929 cells. Immunofluorescence staining was performed to detect the presence of O. tsutsugamushi (magnification, 400×) at day 7 and day 14 post inoculation. (D) qPCR was used to quantify number of Orientia in L929 cells collected in (C) .

    Techniques Used: Infection, Mouse Assay, Real-time Polymerase Chain Reaction, Immunofluorescence, Staining

    36) Product Images from "Silica particles contribute to the procoagulant activity of DNA and polyphosphate isolated using commercial kits"

    Article Title: Silica particles contribute to the procoagulant activity of DNA and polyphosphate isolated using commercial kits

    Journal: Blood

    doi: 10.1182/blood-2017-03-772848

    Procoagulant activities of DNA, polyP, and silica particles. (A-D) Shown are plasma clot times versus concentration, with horizontal dashed lines showing the mean clot time without activator (± standard error of the mean as horizontal dotted lines). (A) Clot times with: HEK 293 cell DNA isolated with DNeasy Blood Tissue (open diamond); HEK 293 cell DNA isolated with phenol/chloroform (solid diamond); NET-derived DNA isolated with DNeasy Blood Tissue kit (open inverted triangle); or NET-derived DNA isolated with phenol/chloroform (solid inverted triangle). Each data set represents the mean clot time for 3 separate purifications as detailed in supplemental Figure 2A (HEK293 cell DNA) and supplemental Figure 2B (NET DNA). (B) Clot times with silica particles: glass milk (solid triangle) or homogenized DNeasy column matrix (open triangle). (C) Clot times with water elutions from 3 different lots of DNeasy columns (open triangle) or 2 different lots of Econospin columns (open circle). On the x-axis, fold concentration refers to the concentration relative to the volume eluted from the Qiagen column, with 1 equaling the original elution volume. (D) Clot times of λ phage DNA before (blue square) or after (open square) repurification on DNeasy columns; or of short-chain polyP before (blue circle) or after (open circle) repurification on DNeasy columns. The purification data sets represent mean clot times for 2 different purifications as detailed in supplemental Figure 4. (E) Clot times of various samples before (red bars) or after (blue bars) digestion with a combination of Benzonase and calf intestine alkaline phosphatase. Samples include buffer control (no activator), 20 µg/mL HEK 293 cell DNA purified using DNeasy, 20 µg/mL NET DNA purified using DNeasy, water applied to DNeasy column as described in panel C (water elution), or 2 µg/mL long-chain polyP. Digestion of DNA by Benzonase and polyP by phosphatase was confirmed by gel electrophoresis (supplemental Figure 1). (F) Clot times of various samples before (red bars) or after (blue bars) acid hydrolysis. Samples include buffer control (no activator), 20 µg/mL HEK 293 cell DNA purified using DNeasy, 20 µg/mL NET DNA purified using DNeasy, water applied to DNeasy column (water elution), 2 µg/mL long-chain polyP, or 5 µg/mL polyguanylate (polyG). (G) Clot times in the presence of varying concentrations of histones with (open triangle) or without (solid triangle) a 10-fold concentrated water elution (prepared as described for panel C). (H) Tissue factor–triggered clot times in the presence of varying concentrations of histones.
    Figure Legend Snippet: Procoagulant activities of DNA, polyP, and silica particles. (A-D) Shown are plasma clot times versus concentration, with horizontal dashed lines showing the mean clot time without activator (± standard error of the mean as horizontal dotted lines). (A) Clot times with: HEK 293 cell DNA isolated with DNeasy Blood Tissue (open diamond); HEK 293 cell DNA isolated with phenol/chloroform (solid diamond); NET-derived DNA isolated with DNeasy Blood Tissue kit (open inverted triangle); or NET-derived DNA isolated with phenol/chloroform (solid inverted triangle). Each data set represents the mean clot time for 3 separate purifications as detailed in supplemental Figure 2A (HEK293 cell DNA) and supplemental Figure 2B (NET DNA). (B) Clot times with silica particles: glass milk (solid triangle) or homogenized DNeasy column matrix (open triangle). (C) Clot times with water elutions from 3 different lots of DNeasy columns (open triangle) or 2 different lots of Econospin columns (open circle). On the x-axis, fold concentration refers to the concentration relative to the volume eluted from the Qiagen column, with 1 equaling the original elution volume. (D) Clot times of λ phage DNA before (blue square) or after (open square) repurification on DNeasy columns; or of short-chain polyP before (blue circle) or after (open circle) repurification on DNeasy columns. The purification data sets represent mean clot times for 2 different purifications as detailed in supplemental Figure 4. (E) Clot times of various samples before (red bars) or after (blue bars) digestion with a combination of Benzonase and calf intestine alkaline phosphatase. Samples include buffer control (no activator), 20 µg/mL HEK 293 cell DNA purified using DNeasy, 20 µg/mL NET DNA purified using DNeasy, water applied to DNeasy column as described in panel C (water elution), or 2 µg/mL long-chain polyP. Digestion of DNA by Benzonase and polyP by phosphatase was confirmed by gel electrophoresis (supplemental Figure 1). (F) Clot times of various samples before (red bars) or after (blue bars) acid hydrolysis. Samples include buffer control (no activator), 20 µg/mL HEK 293 cell DNA purified using DNeasy, 20 µg/mL NET DNA purified using DNeasy, water applied to DNeasy column (water elution), 2 µg/mL long-chain polyP, or 5 µg/mL polyguanylate (polyG). (G) Clot times in the presence of varying concentrations of histones with (open triangle) or without (solid triangle) a 10-fold concentrated water elution (prepared as described for panel C). (H) Tissue factor–triggered clot times in the presence of varying concentrations of histones.

    Techniques Used: Concentration Assay, Isolation, Derivative Assay, Purification, Nucleic Acid Electrophoresis

    37) Product Images from "Development of Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid and Sensitive Identification of Ostrich Meat"

    Article Title: Development of Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid and Sensitive Identification of Ostrich Meat

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0100717

    Amplification signal of ostrich meat DNA using the direct swab and HYPLEX buffer test method. The red curve is the signal of ostrich DNA isolated with DNeasy tissue Isolation Kit (detection time of 6∶18 min). The other positive curves in the oval shap are the direct swab samples with a detection time of 11∶03–12∶18 min. The pink curve is the negative control (see table 3 ).
    Figure Legend Snippet: Amplification signal of ostrich meat DNA using the direct swab and HYPLEX buffer test method. The red curve is the signal of ostrich DNA isolated with DNeasy tissue Isolation Kit (detection time of 6∶18 min). The other positive curves in the oval shap are the direct swab samples with a detection time of 11∶03–12∶18 min. The pink curve is the negative control (see table 3 ).

    Techniques Used: Amplification, Isolation, Negative Control

    38) Product Images from "Cholestyramine as a Promising, Strong Anion Exchange Resin for Direct Capture of Genetic Biomarkers from Raw Pancreatic Fluids"

    Article Title: Cholestyramine as a Promising, Strong Anion Exchange Resin for Direct Capture of Genetic Biomarkers from Raw Pancreatic Fluids

    Journal: Biotechnology and bioengineering

    doi: 10.1002/bit.26207

    (a) Recovered DNA quantities using resin-based capture from endoscopically collected pancreatic fluid samples from 6 patients with suspected chronic pancreatitis at three different time points. (b) Parity plot comparing the amount of DNA recovered using the resin capture approach and DNeasy spin columns. Cholestyramine resin demonstrates a similar performance to the DNeasy spin columns, which represent the state-of-the-art technology for in vitro DNA capture from biological fluids. Samples were processed in duplicate, and error bars represent +/− 1 SD. DNA concentrations were evaluated using the Quantifiler Human DNA Quantification Kit.
    Figure Legend Snippet: (a) Recovered DNA quantities using resin-based capture from endoscopically collected pancreatic fluid samples from 6 patients with suspected chronic pancreatitis at three different time points. (b) Parity plot comparing the amount of DNA recovered using the resin capture approach and DNeasy spin columns. Cholestyramine resin demonstrates a similar performance to the DNeasy spin columns, which represent the state-of-the-art technology for in vitro DNA capture from biological fluids. Samples were processed in duplicate, and error bars represent +/− 1 SD. DNA concentrations were evaluated using the Quantifiler Human DNA Quantification Kit.

    Techniques Used: In Vitro

    39) Product Images from "Species‐level biodiversity assessment using marine environmental DNA metabarcoding requires protocol optimization and standardization, et al. Species‐level biodiversity assessment using marine environmental DNA metabarcoding requires protocol optimization and standardization"

    Article Title: Species‐level biodiversity assessment using marine environmental DNA metabarcoding requires protocol optimization and standardization, et al. Species‐level biodiversity assessment using marine environmental DNA metabarcoding requires protocol optimization and standardization

    Journal: Ecology and Evolution

    doi: 10.1002/ece3.4843

    DNA yield from seven extraction protocols (DNeasy: Qiagen DNeasy Blood Tissue Kit; MO BIO PW: MO BIO PowerWater DNA Isolation Kit; MO BIO PMS: MO BIO PowerMax Soil DNA Isolation Kit; Dnature: Presto™ Mini gDNA Bacteria Kit; PCI: phenol‐chloroform‐isoamyl extraction procedure; Silica: Silica extraction procedure; and Magnetic beads: Magnetic beads extraction procedure) obtained from 1,000 ml technical replicates filtered through 1.2‐µm cellulose‐nitrate filters. Error bars show 95% confidence intervals. Letters indicate significant differences among groups based on Tukey–Kramer's test (α = 0.05)
    Figure Legend Snippet: DNA yield from seven extraction protocols (DNeasy: Qiagen DNeasy Blood Tissue Kit; MO BIO PW: MO BIO PowerWater DNA Isolation Kit; MO BIO PMS: MO BIO PowerMax Soil DNA Isolation Kit; Dnature: Presto™ Mini gDNA Bacteria Kit; PCI: phenol‐chloroform‐isoamyl extraction procedure; Silica: Silica extraction procedure; and Magnetic beads: Magnetic beads extraction procedure) obtained from 1,000 ml technical replicates filtered through 1.2‐µm cellulose‐nitrate filters. Error bars show 95% confidence intervals. Letters indicate significant differences among groups based on Tukey–Kramer's test (α = 0.05)

    Techniques Used: DNA Extraction, Magnetic Beads

    The average (a) OTU and (b) taxon richness obtained per replicate for each of the four assays between the optimal (blue; 1.2‐µm cellulose‐nitrate filter and Qiagen's DNeasy Blood Tissue Kit) and low‐performance (gold; 1.2‐µm polycarbonate filter and MO BIO's PowerMax Soil) protocol. Error bars show 95% confidence intervals. T test significance is depicted by: *** p
    Figure Legend Snippet: The average (a) OTU and (b) taxon richness obtained per replicate for each of the four assays between the optimal (blue; 1.2‐µm cellulose‐nitrate filter and Qiagen's DNeasy Blood Tissue Kit) and low‐performance (gold; 1.2‐µm polycarbonate filter and MO BIO's PowerMax Soil) protocol. Error bars show 95% confidence intervals. T test significance is depicted by: *** p

    Techniques Used:

    Observed taxa in each of the four assays. Filled and unfilled rectangles indicate taxon presence or absence. Taxa show a pair of rectangles per assay, representing the low‐performance (PCPMS; 1.2‐µm polycarbonate filter and MO BIO's PowerMax Soil) and the optimal (CNQ; 1.2‐µm cellulose‐nitrate filter and Qiagen's DNeasy Blood Tissue Kit) treatment. Rectangular presence indicates the amplification range of each assay
    Figure Legend Snippet: Observed taxa in each of the four assays. Filled and unfilled rectangles indicate taxon presence or absence. Taxa show a pair of rectangles per assay, representing the low‐performance (PCPMS; 1.2‐µm polycarbonate filter and MO BIO's PowerMax Soil) and the optimal (CNQ; 1.2‐µm cellulose‐nitrate filter and Qiagen's DNeasy Blood Tissue Kit) treatment. Rectangular presence indicates the amplification range of each assay

    Techniques Used: Amplification

    40) Product Images from "Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows, et al. Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows"

    Article Title: Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows, et al. Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows

    Journal: Ecology and Evolution

    doi: 10.1002/ece3.6540

    Detection rates of the twelve tested workflows for water samples (a) and sediment samples (b), shown is the percentage of positive samples among the 16 PCR reactions of each workflow (CB/ C = CTAB DNA extraction, DN = DNeasy Blood Tissue Kit, HS/ H = high salt DNA extraction, N = Nucleo Spin Soil Kit)
    Figure Legend Snippet: Detection rates of the twelve tested workflows for water samples (a) and sediment samples (b), shown is the percentage of positive samples among the 16 PCR reactions of each workflow (CB/ C = CTAB DNA extraction, DN = DNeasy Blood Tissue Kit, HS/ H = high salt DNA extraction, N = Nucleo Spin Soil Kit)

    Techniques Used: Polymerase Chain Reaction, DNA Extraction

    Total DNA yield (a) and threshold cycle values (b) measured in water samples of the twelve tested workflows (CB = CTAB DNA extraction, DN = DNeasy Blood Tissue Kit, HS = high salt DNA extraction). C t values are inversely proportional to the target DNA amount of a sample; thus, the lower the C t value, the higher is the target DNA amount
    Figure Legend Snippet: Total DNA yield (a) and threshold cycle values (b) measured in water samples of the twelve tested workflows (CB = CTAB DNA extraction, DN = DNeasy Blood Tissue Kit, HS = high salt DNA extraction). C t values are inversely proportional to the target DNA amount of a sample; thus, the lower the C t value, the higher is the target DNA amount

    Techniques Used: DNA Extraction

    Related Articles

    DNA Extraction:

    Article Title: The Anatomy to Genomics (ATG) Start Genetics medical school initiative: incorporating exome sequencing data from cadavers used for Anatomy instruction into the first year curriculum
    Article Snippet: .. Samples of tissue (1 cm3 ) were finely minced using a scalpel blade and then subjected to DNA isolation using either the QIAamp DNA FFPE Tissue Kit or the Qiagen DNeasy Blood & Tissue Kit (Qiagen, Inc.). .. For the FFPE procedure, extraction with xylene was omitted but incubation at 90 °C to reverse formalin crosslinking was performed.

    Article Title: Comparison of DNA extraction methods for non-marine molluscs: Is modified CTAB DNA extraction method more efficient than DNA extraction kits?
    Article Snippet: .. We have also compared its performance with Qiagen DNeasy® Blood and Tissue Kit since it is one of the most widely used commercially available kits for DNA extraction. .. This study included 23 representative individuals belonging to 13 families, 18 genera and 23 species collected from the Western Ghats of India ( ).

    Formalin-fixed Paraffin-Embedded:

    Article Title: The Anatomy to Genomics (ATG) Start Genetics medical school initiative: incorporating exome sequencing data from cadavers used for Anatomy instruction into the first year curriculum
    Article Snippet: .. Samples of tissue (1 cm3 ) were finely minced using a scalpel blade and then subjected to DNA isolation using either the QIAamp DNA FFPE Tissue Kit or the Qiagen DNeasy Blood & Tissue Kit (Qiagen, Inc.). .. For the FFPE procedure, extraction with xylene was omitted but incubation at 90 °C to reverse formalin crosslinking was performed.

    Isolation:

    Article Title: A Novel Molecular Test to Diagnose Canine Visceral Leishmaniasis at the Point of Care
    Article Snippet: To estimate the analytical sensitivity of quantitative real-time PCR (qPCR) and RPA-LF, DNA was extracted from 1 × 105 cultured promastigotes spiked in dog blood and then serially diluted to the equivalent of 0.01 parasites per reaction. .. DNA was isolated from blood or tissue samples using the QIAGEN DNeasy blood and tissue extraction kit (Qiagen, Valencia, CA) following the instructions of the vendor. .. In brief, DNA was isolated from Whatman FTA paper as follows: two 6-mm disposable skin biopsy punches were used to cut the absorbed/dried samples and the filter disc was resuspended in 200 μL water and placed in a heat block at 96°C for 2 minutes.

    Protein Extraction:

    Article Title: Tissue Fractionation by Hydrostatic Pressure Cycling Technology: The Unified Sample Preparation Technique for Systems Biology Studies
    Article Snippet: .. We found that the combination of the PCT/ProteoSolve-SB method for cell disruption and protein extraction, together with the DNeasy kit for DNA purification, resulted in very good simultaneous recovery of DNA and protein from cells ( ). .. In addition, the same sequential protocol was successfully applied to protein and DNA extraction from liver tissue ( ).

    DNA Purification:

    Article Title: Tissue Fractionation by Hydrostatic Pressure Cycling Technology: The Unified Sample Preparation Technique for Systems Biology Studies
    Article Snippet: .. We found that the combination of the PCT/ProteoSolve-SB method for cell disruption and protein extraction, together with the DNeasy kit for DNA purification, resulted in very good simultaneous recovery of DNA and protein from cells ( ). .. In addition, the same sequential protocol was successfully applied to protein and DNA extraction from liver tissue ( ).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    Qiagen dneasy blood and tissue kit
    Comparison of average sequence quality between CTAB and <t>Qiagen®</t> <t>DNeasy</t> Blood and Tissue Kit extracted amplified genomic DNA
    Dneasy Blood And Tissue Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dneasy blood and tissue kit/product/Qiagen
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dneasy blood and tissue kit - by Bioz Stars, 2021-03
    97/100 stars
      Buy from Supplier

    Image Search Results


    Comparison of average sequence quality between CTAB and Qiagen® DNeasy Blood and Tissue Kit extracted amplified genomic DNA

    Journal: bioRxiv

    Article Title: Comparison of DNA extraction methods for non-marine molluscs: Is modified CTAB DNA extraction method more efficient than DNA extraction kits?

    doi: 10.1101/863167

    Figure Lengend Snippet: Comparison of average sequence quality between CTAB and Qiagen® DNeasy Blood and Tissue Kit extracted amplified genomic DNA

    Article Snippet: We have also compared its performance with Qiagen DNeasy® Blood and Tissue Kit since it is one of the most widely used commercially available kits for DNA extraction.

    Techniques: Sequencing, Amplification

    Chromatogram depicting 16S gene sequence quality in Qiagen ® DNeasy Blood and Tissue Kit extracted DNA.

    Journal: bioRxiv

    Article Title: Comparison of DNA extraction methods for non-marine molluscs: Is modified CTAB DNA extraction method more efficient than DNA extraction kits?

    doi: 10.1101/863167

    Figure Lengend Snippet: Chromatogram depicting 16S gene sequence quality in Qiagen ® DNeasy Blood and Tissue Kit extracted DNA.

    Article Snippet: We have also compared its performance with Qiagen DNeasy® Blood and Tissue Kit since it is one of the most widely used commercially available kits for DNA extraction.

    Techniques: Sequencing

    DNeasy treated samples. (a) Two LightCycler PCR runs, first one with standard dilution series of human Hep2 DNA before (flat negative curves) and after DNeasy (indicated with squares and triangles), showed on the left and second with DNA free water before (marked with squares) and after DNeasy (triangles) on the right. (b) Melting curve analysis of DNeasy treated H 2 O (triangles), Hep2 DNA (squares) and clinical sample.

    Journal: BMC Microbiology

    Article Title: Development of real-time PCR for detection of Mycoplasma hominis

    doi: 10.1186/1471-2180-4-35

    Figure Lengend Snippet: DNeasy treated samples. (a) Two LightCycler PCR runs, first one with standard dilution series of human Hep2 DNA before (flat negative curves) and after DNeasy (indicated with squares and triangles), showed on the left and second with DNA free water before (marked with squares) and after DNeasy (triangles) on the right. (b) Melting curve analysis of DNeasy treated H 2 O (triangles), Hep2 DNA (squares) and clinical sample.

    Article Snippet: Twenty-five μl of each dilution and DNA free double distilled water were then treated with DNeasy™ Tissue Kit (QIAGEN GmbH, Hilden, Germany) procedure.

    Techniques: Polymerase Chain Reaction

    Sensitivity of recombinase polymerase amplification–lateral flow (RPA-LF) to detect Leishmania infantum compared with real-time polymerase chain reaction (PCR) used as gold standard. Tenfold serial dilutions of L. infantum promastigotes in dog blood were extracted using Qiagen ® DNeasy blood and tissue kit and detected by real-time quantitative PCR (SYBRgreen) or RPA-LF. Parasite dilutions: 1 = 10 5 , 2 = 10 4 , 3 = 10 3 , 4 = 10 2 , 5 = 10, 6 = 1, and 7 = 0.1 parasites and Bl = uninfected dog blood. The top band is the control band; the lower band is the test band. This is a representative figure of two similar assays.

    Journal: The American Journal of Tropical Medicine and Hygiene

    Article Title: A Novel Molecular Test to Diagnose Canine Visceral Leishmaniasis at the Point of Care

    doi: 10.4269/ajtmh.15-0145

    Figure Lengend Snippet: Sensitivity of recombinase polymerase amplification–lateral flow (RPA-LF) to detect Leishmania infantum compared with real-time polymerase chain reaction (PCR) used as gold standard. Tenfold serial dilutions of L. infantum promastigotes in dog blood were extracted using Qiagen ® DNeasy blood and tissue kit and detected by real-time quantitative PCR (SYBRgreen) or RPA-LF. Parasite dilutions: 1 = 10 5 , 2 = 10 4 , 3 = 10 3 , 4 = 10 2 , 5 = 10, 6 = 1, and 7 = 0.1 parasites and Bl = uninfected dog blood. The top band is the control band; the lower band is the test band. This is a representative figure of two similar assays.

    Article Snippet: DNA was isolated from blood or tissue samples using the QIAGEN DNeasy blood and tissue extraction kit (Qiagen, Valencia, CA) following the instructions of the vendor.

    Techniques: Recombinase Polymerase Amplification, Flow Cytometry, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction

    A: DNA and protein recovery from the cell culture. one aliquot of cells was processed with PCT and ProteoSolve-SB (P). DNA was extracted from the solid phase using the DNeasy kit. The control aliquot of cells was processed directly with the qiagen DNeasy

    Journal: Journal of Biomolecular Techniques : JBT

    Article Title: Tissue Fractionation by Hydrostatic Pressure Cycling Technology: The Unified Sample Preparation Technique for Systems Biology Studies

    doi:

    Figure Lengend Snippet: A: DNA and protein recovery from the cell culture. one aliquot of cells was processed with PCT and ProteoSolve-SB (P). DNA was extracted from the solid phase using the DNeasy kit. The control aliquot of cells was processed directly with the qiagen DNeasy

    Article Snippet: We found that the combination of the PCT/ProteoSolve-SB method for cell disruption and protein extraction, together with the DNeasy kit for DNA purification, resulted in very good simultaneous recovery of DNA and protein from cells ( ).

    Techniques: Cell Culture