cat no 69504 (Qiagen)
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cat no 69504
Cat No 69504, supplied by Qiagen, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cat no 69504/product/Qiagen
Average 77 stars, based on 3 article reviews
Price from $9.99 to $1999.99
Cat No 69504, supplied by Qiagen, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cat no 69504/product/Qiagen
Average 77 stars, based on 3 article reviews
Price from $9.99 to $1999.99
cat no 69504 - by Bioz Stars,
2020-01
77/100 stars
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Methylation Sequencing:Article Title: Dynamic Imprinting of the Treg Cell-Specific Epigenetic Signature in Developing Thymic Regulatory T Cells Article Snippet: DNA Methylation Analysis Genomic DNA was isolated from sorted CD4SP thymocyte subsets using the Clone Assay:Article Title: A human expression system based on HEK293 for the stable production of recombinant erythropoietin Article Snippet: Sequencing of targeted GLUL genomic locus Genomic DNA was isolated from potential GLUL -KO cell lines using Amplification:Article Title: HIF-1α protects against oxidative stress by directly targeting mitochondria Article Snippet: .. 2.6 Mitochondrial DNA amplification Total cellular DNA was isolated using Article Title: Immunodominant, protective response to the parasite Toxoplasma gondii requires antigen processing in the endoplasmic reticulum Article Snippet: Genomic DNA was extracted from less than 10 mg ground tissue with the Article Title: Diversity, Activity, and Evolution of CRISPR Loci in Streptococcus thermophilus ▿ ▿ † Article Snippet: S. thermophilus genomic DNA was extracted and purified by using a Synthesized:Article Title: HIF-1α protects against oxidative stress by directly targeting mitochondria Article Snippet: 2.6 Mitochondrial DNA amplification Total cellular DNA was isolated using Article Title: Expression quantitative trait loci detected in cell lines are often present in primary tissues Article Snippet: We extracted DNA from each tissue using the Qiagen Cytometry:Article Title: Immunodominant, protective response to the parasite Toxoplasma gondii requires antigen processing in the endoplasmic reticulum Article Snippet: The number of infected splenocytes and peritoneal macrophages was determined by measurement of the percentage of GFP+ cells by flow cytometry. .. Genomic DNA was extracted from less than 10 mg ground tissue with the Quantitative RT-PCR:Article Title: HIF-1α protects against oxidative stress by directly targeting mitochondria Article Snippet: 2.6 Mitochondrial DNA amplification Total cellular DNA was isolated using Real-time Polymerase Chain Reaction:Article Title: Highly sensitive detection of sentinel lymph node metastasis of breast cancer by digital PCR for RASSF1A methylation Article Snippet: DNA and mRNA were extracted from 10E+6 cells from each cell line using the Article Title: Efficacy of three innovative bacterin vaccines against experimental infection with Mycoplasma hyopneumoniae Article Snippet: .. Deoxyribonucleic acid was extracted from the BAL fluid using a commercial kit ( Article Title: HIF-1α protects against oxidative stress by directly targeting mitochondria Article Snippet: 2.6 Mitochondrial DNA amplification Total cellular DNA was isolated using Incubation:Article Title: Application of different DNA extraction procedures, library preparation protocols and sequencing platforms: impact on sequencing results Article Snippet: At the end of the incubation period, 500 μl of broth were centrifuged at 5000 x g for 10’. .. Pellets obtained from cultures BACT1 to BACT4 were suspended in 180 μl of ATL buffer and submitted to DNA extraction using the Article Title: What drives phenotypic divergence among coral clonemates of Acropora palmata?. What drives phenotypic divergence among coral clonemates of Acropora palmata? Article Snippet: 2.4 Methylrad library preparation and bioinformatics Coral tissue samples were snap frozen in liquid nitrogen and kept at −80°C before extraction using the Article Title: Efficacy of three innovative bacterin vaccines against experimental infection with Mycoplasma hyopneumoniae Article Snippet: Deoxyribonucleic acid was extracted from the BAL fluid using a commercial kit ( Infection:Article Title: Efficacy of three innovative bacterin vaccines against experimental infection with Mycoplasma hyopneumoniae Article Snippet: Quantitative PCR for M. hyopneumoniae DNA and routine bacteriological culture on bronchoalveolar lavage fluid Two weeks after challenge infection (D42), bronchoalveolar lavage fluid was collected from each pig by inserting a catheter (Portex® Dog Catheter with Female Luer Mount, Smiths Medical International Ltd., Kent, UK) in the trachea and flushing the lungs with 20 mL sterile PBS [ ]. .. Deoxyribonucleic acid was extracted from the BAL fluid using a commercial kit ( Article Title: Immunodominant, protective response to the parasite Toxoplasma gondii requires antigen processing in the endoplasmic reticulum Article Snippet: The number of infected splenocytes and peritoneal macrophages was determined by measurement of the percentage of GFP+ cells by flow cytometry. .. Genomic DNA was extracted from less than 10 mg ground tissue with the Expressing:Article Title: Highly sensitive detection of sentinel lymph node metastasis of breast cancer by digital PCR for RASSF1A methylation Article Snippet: DNA and mRNA were extracted from 10E+6 cells from each cell line using the Article Title: Expression quantitative trait loci detected in cell lines are often present in primary tissues Article Snippet: Given we are primarily interested in the effect of cis regulatory variation, and not the genetic basis of expression covariation among tissues, having largely non-overlapping sets of individuals is preferable to having all tissues from each individual because non-overlapping sets will reduce the impact of individual-specific effects, such as environmental effects, which otherwise could confound the analysis. .. We extracted DNA from each tissue using the Qiagen DNA Methylation Assay:Article Title: Dynamic Imprinting of the Treg Cell-Specific Epigenetic Signature in Developing Thymic Regulatory T Cells Article Snippet: .. DNA Methylation Analysis Genomic DNA was isolated from sorted CD4SP thymocyte subsets using the Flow Cytometry:Article Title: Immunodominant, protective response to the parasite Toxoplasma gondii requires antigen processing in the endoplasmic reticulum Article Snippet: The number of infected splenocytes and peritoneal macrophages was determined by measurement of the percentage of GFP+ cells by flow cytometry. .. Genomic DNA was extracted from less than 10 mg ground tissue with the Cell Culture:Article Title: Highly sensitive detection of sentinel lymph node metastasis of breast cancer by digital PCR for RASSF1A methylation Article Snippet: Quantification of methylated RASSF1A and CK19 mRNA in breast cancer cell lines A total of six breast cancer cell lines (MCF7, MDA-MB-361, BT474, MDA-MB-453, MDA-MB-231 and BT20) were cultured in DMEM (Sigma-Aldrich; Merck KGaA), and five (ZR75-1, T47D, ZR75-30, SKBR3 and AU565) were cultured in RPMI-1640 (Sigma-Aldrich; Merck KGaA) at 37°C (5% CO2 ) in a humidified atmosphere. .. DNA and mRNA were extracted from 10E+6 cells from each cell line using the Article Title: Establishment of a novel human CIC-DUX4 sarcoma cell line, Kitra-SRS, with autocrine IGF-1R activation and metastatic potential to the lungs Article Snippet: .. Authentication and quality control of the established cell line Genomic DNA was extracted from the tumour tissues or cultured cells using the DNA Sequencing:Article Title: Diversity, Activity, and Evolution of CRISPR Loci in Streptococcus thermophilus ▿ ▿ † Article Snippet: Paragraph title: DNA sequencing of S. thermophilus CRISPR loci. ... S. thermophilus genomic DNA was extracted and purified by using a Sequencing:Article Title: A human expression system based on HEK293 for the stable production of recombinant erythropoietin Article Snippet: .. Sequencing of targeted GLUL genomic locus Genomic DNA was isolated from potential GLUL -KO cell lines using Article Title: Diversity, Activity, and Evolution of CRISPR Loci in Streptococcus thermophilus ▿ ▿ † Article Snippet: S. thermophilus genomic DNA was extracted and purified by using a Sonication:Article Title: Prognostic implications of 5-hydroxymethylcytosines from circulating cell-free DNA in diffuse large B-cell lymphoma Article Snippet: .. Genomic DNA from cfDNA-paired tumor blocks for 7 patients was isolated (30-50 ng per sample) using a DNA Extraction:Article Title: Application of different DNA extraction procedures, library preparation protocols and sequencing platforms: impact on sequencing results Article Snippet: .. Pellets obtained from cultures BACT1 to BACT4 were suspended in 180 μl of ATL buffer and submitted to DNA extraction using the Article Title: Ancient mitogenomics clarifies radiation of extinct Mascarene giant tortoises (Cylindraspis spp.) Article Snippet: Negative controls (water blanks) were included during DNA extraction and library preparation and screened for evidence of contamination. .. DNA of the historic tissue sample was extracted using the Methylation:Article Title: Highly sensitive detection of sentinel lymph node metastasis of breast cancer by digital PCR for RASSF1A methylation Article Snippet: Paragraph title: Quantification of methylated RASSF1A and CK19 mRNA in breast cancer cell lines ... DNA and mRNA were extracted from 10E+6 cells from each cell line using the Article Title: Dynamic Imprinting of the Treg Cell-Specific Epigenetic Signature in Developing Thymic Regulatory T Cells Article Snippet: DNA Methylation Analysis Genomic DNA was isolated from sorted CD4SP thymocyte subsets using the Multiple Displacement Amplification:Article Title: Highly sensitive detection of sentinel lymph node metastasis of breast cancer by digital PCR for RASSF1A methylation Article Snippet: Quantification of methylated RASSF1A and CK19 mRNA in breast cancer cell lines A total of six breast cancer cell lines (MCF7, MDA-MB-361, BT474, MDA-MB-453, MDA-MB-231 and BT20) were cultured in DMEM (Sigma-Aldrich; Merck KGaA), and five (ZR75-1, T47D, ZR75-30, SKBR3 and AU565) were cultured in RPMI-1640 (Sigma-Aldrich; Merck KGaA) at 37°C (5% CO2 ) in a humidified atmosphere. .. DNA and mRNA were extracted from 10E+6 cells from each cell line using the Isolation:Article Title: A human expression system based on HEK293 for the stable production of recombinant erythropoietin Article Snippet: .. Sequencing of targeted GLUL genomic locus Genomic DNA was isolated from potential GLUL -KO cell lines using Article Title: HIF-1α protects against oxidative stress by directly targeting mitochondria Article Snippet: .. 2.6 Mitochondrial DNA amplification Total cellular DNA was isolated using Article Title: Dynamic Imprinting of the Treg Cell-Specific Epigenetic Signature in Developing Thymic Regulatory T Cells Article Snippet: .. DNA Methylation Analysis Genomic DNA was isolated from sorted CD4SP thymocyte subsets using the Article Title: Prognostic implications of 5-hydroxymethylcytosines from circulating cell-free DNA in diffuse large B-cell lymphoma Article Snippet: .. Genomic DNA from cfDNA-paired tumor blocks for 7 patients was isolated (30-50 ng per sample) using a Size-exclusion Chromatography:Article Title: Highly sensitive detection of sentinel lymph node metastasis of breast cancer by digital PCR for RASSF1A methylation Article Snippet: DNA and mRNA were extracted from 10E+6 cells from each cell line using the Article Title: Ancient mitogenomics clarifies radiation of extinct Mascarene giant tortoises (Cylindraspis spp.) Article Snippet: DNA of the historic tissue sample was extracted using the Mouse Assay:Article Title: Dynamic Imprinting of the Treg Cell-Specific Epigenetic Signature in Developing Thymic Regulatory T Cells Article Snippet: DNA Methylation Analysis Genomic DNA was isolated from sorted CD4SP thymocyte subsets using the Polymerase Chain Reaction:Article Title: Highly sensitive detection of sentinel lymph node metastasis of breast cancer by digital PCR for RASSF1A methylation Article Snippet: DNA and mRNA were extracted from 10E+6 cells from each cell line using the Article Title: A human expression system based on HEK293 for the stable production of recombinant erythropoietin Article Snippet: Sequencing of targeted GLUL genomic locus Genomic DNA was isolated from potential GLUL -KO cell lines using Article Title: HIF-1α protects against oxidative stress by directly targeting mitochondria Article Snippet: 2.6 Mitochondrial DNA amplification Total cellular DNA was isolated using Article Title: Taxonomic revision of the endemic Cameroonian freshwater crab genus Louisea Cumberlidge, 1994 ( Crustacea, Decapoda, Brachyura, Potamonautidae), with descriptions of two new species from Nkongsamba and Yabassi Article Snippet: Genomic DNA was extracted from a tissue sample of up to 25 mg cut from the pereiopod muscle of 70% ethanol-preserved specimens using the Qiagen Article Title: Immunodominant, protective response to the parasite Toxoplasma gondii requires antigen processing in the endoplasmic reticulum Article Snippet: Parasite burden in spleen, liver and brain was assessed by semiquantitative PCR as described . .. Genomic DNA was extracted from less than 10 mg ground tissue with the Article Title: Diversity, Activity, and Evolution of CRISPR Loci in Streptococcus thermophilus ▿ ▿ † Article Snippet: S. thermophilus genomic DNA was extracted and purified by using a Construct:Article Title: Prognostic implications of 5-hydroxymethylcytosines from circulating cell-free DNA in diffuse large B-cell lymphoma Article Snippet: Genomic DNA from cfDNA-paired tumor blocks for 7 patients was isolated (30-50 ng per sample) using a CRISPR:Article Title: Diversity, Activity, and Evolution of CRISPR Loci in Streptococcus thermophilus ▿ ▿ † Article Snippet: Paragraph title: DNA sequencing of S. thermophilus CRISPR loci. ... S. thermophilus genomic DNA was extracted and purified by using a Purification:Article Title: Diversity, Activity, and Evolution of CRISPR Loci in Streptococcus thermophilus ▿ ▿ † Article Snippet: .. S. thermophilus genomic DNA was extracted and purified by using a Chromatin Immunoprecipitation:Article Title: The Genetic Architecture of Bovine Telomere Length in Early Life and Association With Animal Fitness Article Snippet: Genotyping DNA was extracted from whole blood using the Software:Article Title: The Genetic Architecture of Bovine Telomere Length in Early Life and Association With Animal Fitness Article Snippet: Genotyping DNA was extracted from whole blood using the Multiplex Assay:Article Title: Establishment of a novel human CIC-DUX4 sarcoma cell line, Kitra-SRS, with autocrine IGF-1R activation and metastatic potential to the lungs Article Snippet: Authentication and quality control of the established cell line Genomic DNA was extracted from the tumour tissues or cultured cells using the Sample Prep:Article Title: Prognostic implications of 5-hydroxymethylcytosines from circulating cell-free DNA in diffuse large B-cell lymphoma Article Snippet: Paragraph title: Sample preparation and the 5hmC-Seal profiling ... Genomic DNA from cfDNA-paired tumor blocks for 7 patients was isolated (30-50 ng per sample) using a Spectrophotometry:Article Title: HIF-1α protects against oxidative stress by directly targeting mitochondria Article Snippet: .. 2.6 Mitochondrial DNA amplification Total cellular DNA was isolated using Article Title: Dynamic Imprinting of the Treg Cell-Specific Epigenetic Signature in Developing Thymic Regulatory T Cells Article Snippet: DNA Methylation Analysis Genomic DNA was isolated from sorted CD4SP thymocyte subsets using the Sampling:Article Title: Ancient mitogenomics clarifies radiation of extinct Mascarene giant tortoises (Cylindraspis spp.) Article Snippet: Processing of historic and subfossil Cylindraspis samples The 18 subfossil bone specimens were sampled using a Dremel drill equipped either with a core sampling bit or diamond circular saw bit, depending on the specimen. .. DNA of the historic tissue sample was extracted using the Concentration Assay:Article Title: Dynamic Imprinting of the Treg Cell-Specific Epigenetic Signature in Developing Thymic Regulatory T Cells Article Snippet: DNA Methylation Analysis Genomic DNA was isolated from sorted CD4SP thymocyte subsets using the Gel Extraction:Article Title: A human expression system based on HEK293 for the stable production of recombinant erythropoietin Article Snippet: Sequencing of targeted GLUL genomic locus Genomic DNA was isolated from potential GLUL -KO cell lines using |