dneasy blood and tissue kit (Qiagen)
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DNeasy Blood Tissue Kit
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For spin column or 96 well purification of total DNA from animal blood and tissues and from cells yeast bacteria or viruses Kit contents Qiagen DNeasy Blood Tissue Kit 50 preps 100L 25mg Sample 100 to 200L Elution Volume Blood Tissue Sample Total DNA Purification Silica Technology Spin Column Format 6g 30g Yield Manual Processing For Purification of Total DNA from Animal Blood and Tissues and from Cells Yeast Bacteria or Viruses Ideal for PCR Real time PCR Genotyping Includes 50 DNeasy Mini Spin Columns Proteinase K Buffers 2mL Collection Tubes Benefits Standardized method for a variety of sample types High yields even from specialized samples High quality DNA Optimized protocols for a range of starting materials Spin column and 96 well high throughput form
Catalog Number:
69504
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163
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DNeasy Blood Tissue Kits
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Structured Review
For spin column or 96 well purification of total DNA from animal blood and tissues and from cells yeast bacteria or viruses Kit contents Qiagen DNeasy Blood Tissue Kit 50 preps 100L 25mg Sample 100 to 200L Elution Volume Blood Tissue Sample Total DNA Purification Silica Technology Spin Column Format 6g 30g Yield Manual Processing For Purification of Total DNA from Animal Blood and Tissues and from Cells Yeast Bacteria or Viruses Ideal for PCR Real time PCR Genotyping Includes 50 DNeasy Mini Spin Columns Proteinase K Buffers 2mL Collection Tubes Benefits Standardized method for a variety of sample types High yields even from specialized samples High quality DNA Optimized protocols for a range of starting materials Spin column and 96 well high throughput form
https://www.bioz.com/result/dneasy blood and tissue kit/product/Qiagen
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Price from $9.99 to $1999.99
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1) Product Images from "Comparison of DNA extraction methods for non-marine molluscs: Is modified CTAB DNA extraction method more efficient than DNA extraction kits?"
Article Title: Comparison of DNA extraction methods for non-marine molluscs: Is modified CTAB DNA extraction method more efficient than DNA extraction kits?
Journal: bioRxiv
doi: 10.1101/863167
Figure Legend Snippet: Comparison of average sequence quality between CTAB and Qiagen® DNeasy Blood and Tissue Kit extracted amplified genomic DNA
Techniques Used: Sequencing, Amplification
Figure Legend Snippet: Chromatogram depicting 16S gene sequence quality in Qiagen ® DNeasy Blood and Tissue Kit extracted DNA.
Techniques Used: Sequencing
2) Product Images from "Development of real-time PCR for detection of Mycoplasma hominis"
Article Title: Development of real-time PCR for detection of Mycoplasma hominis
Journal: BMC Microbiology
doi: 10.1186/1471-2180-4-35
Figure Legend Snippet: DNeasy treated samples. (a) Two LightCycler PCR runs, first one with standard dilution series of human Hep2 DNA before (flat negative curves) and after DNeasy (indicated with squares and triangles), showed on the left and second with DNA free water before (marked with squares) and after DNeasy (triangles) on the right. (b) Melting curve analysis of DNeasy treated H 2 O (triangles), Hep2 DNA (squares) and clinical sample.
Techniques Used: Polymerase Chain Reaction
3) Product Images from "A Novel Molecular Test to Diagnose Canine Visceral Leishmaniasis at the Point of Care"
Article Title: A Novel Molecular Test to Diagnose Canine Visceral Leishmaniasis at the Point of Care
Journal: The American Journal of Tropical Medicine and Hygiene
doi: 10.4269/ajtmh.15-0145
Figure Legend Snippet: Sensitivity of recombinase polymerase amplification–lateral flow (RPA-LF) to detect Leishmania infantum compared with real-time polymerase chain reaction (PCR) used as gold standard. Tenfold serial dilutions of L. infantum promastigotes in dog blood were extracted using Qiagen ® DNeasy blood and tissue kit and detected by real-time quantitative PCR (SYBRgreen) or RPA-LF. Parasite dilutions: 1 = 10 5 , 2 = 10 4 , 3 = 10 3 , 4 = 10 2 , 5 = 10, 6 = 1, and 7 = 0.1 parasites and Bl = uninfected dog blood. The top band is the control band; the lower band is the test band. This is a representative figure of two similar assays.
Techniques Used: Recombinase Polymerase Amplification, Flow Cytometry, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction
4) Product Images from "Tissue Fractionation by Hydrostatic Pressure Cycling Technology: The Unified Sample Preparation Technique for Systems Biology Studies"
Article Title: Tissue Fractionation by Hydrostatic Pressure Cycling Technology: The Unified Sample Preparation Technique for Systems Biology Studies
Journal: Journal of Biomolecular Techniques : JBT
doi:
Figure Legend Snippet: A: DNA and protein recovery from the cell culture. one aliquot of cells was processed with PCT and ProteoSolve-SB (P). DNA was extracted from the solid phase using the DNeasy kit. The control aliquot of cells was processed directly with the qiagen DNeasy
Techniques Used: Cell Culture
5) Product Images from "The Anatomy to Genomics (ATG) Start Genetics medical school initiative: incorporating exome sequencing data from cadavers used for Anatomy instruction into the first year curriculum"
Article Title: The Anatomy to Genomics (ATG) Start Genetics medical school initiative: incorporating exome sequencing data from cadavers used for Anatomy instruction into the first year curriculum
Journal: BMC Medical Genomics
doi: 10.1186/s12920-016-0223-4
Figure Legend Snippet: Agarose gel electrophoresis of DNA isolated from cadaver tissues using the DNeasy Blood Tissue Kit. MW = Quick-load 1 kb DNA Ladder (New England BioLabs). Lane 1: Liver without RNAse treatment. Lane 2: Heart without RNAse treatment. Lane 3: Liver with RNAse treatment. Lane 4: Heart with RNAse treatment. Lane 5: Skeletal Muscle with RNAse treatment. Lane 6: Skin with RNAse treatment
Techniques Used: Agarose Gel Electrophoresis, Isolation
Figure Legend Snippet: Agarose gel electrophoresis of DNA isolated from cadaver tissues. MW = GeneRuler 1 kb Plus DNA Ladder (Thermo Fisher Scientific). Lane 1: Heart DNA extracted with DNeasy Blood Tissue Kit. Lane 2: Liver DNA extracted with DNeasy Blood Tissue Kit. Lane 3: Heart DNA extracted with FFPE kit. Lane 4: Liver DNA extracted with FFPE kit
Techniques Used: Agarose Gel Electrophoresis, Isolation, Formalin-fixed Paraffin-Embedded
6) Product Images from "Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows, et al. Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows"
Article Title: Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows, et al. Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows
Journal: Ecology and Evolution
doi: 10.1002/ece3.6540
Figure Legend Snippet: Detection rates of the twelve tested workflows for water samples (a) and sediment samples (b), shown is the percentage of positive samples among the 16 PCR reactions of each workflow (CB/ C = CTAB DNA extraction, DN = DNeasy Blood Tissue Kit, HS/ H = high salt DNA extraction, N = Nucleo Spin Soil Kit)
Techniques Used: Polymerase Chain Reaction, DNA Extraction
Figure Legend Snippet: Total DNA yield (a) and threshold cycle values (b) measured in water samples of the twelve tested workflows (CB = CTAB DNA extraction, DN = DNeasy Blood Tissue Kit, HS = high salt DNA extraction). C t values are inversely proportional to the target DNA amount of a sample; thus, the lower the C t value, the higher is the target DNA amount
Techniques Used: DNA Extraction
7) Product Images from "Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows, et al. Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows"
Article Title: Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows, et al. Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows
Journal: Ecology and Evolution
doi: 10.1002/ece3.6540
Figure Legend Snippet: Detection rates of the twelve tested workflows for water samples (a) and sediment samples (b), shown is the percentage of positive samples among the 16 PCR reactions of each workflow (CB/ C = CTAB DNA extraction, DN = DNeasy Blood Tissue Kit, HS/ H = high salt DNA extraction, N = Nucleo Spin Soil Kit)
Techniques Used: Polymerase Chain Reaction, DNA Extraction
Figure Legend Snippet: Total DNA yield (a) and threshold cycle values (b) measured in water samples of the twelve tested workflows (CB = CTAB DNA extraction, DN = DNeasy Blood Tissue Kit, HS = high salt DNA extraction). C t values are inversely proportional to the target DNA amount of a sample; thus, the lower the C t value, the higher is the target DNA amount
Techniques Used: DNA Extraction
8) Product Images from "Comparison of DNA extraction methods for non-marine molluscs: Is modified CTAB DNA extraction method more efficient than DNA extraction kits?"
Article Title: Comparison of DNA extraction methods for non-marine molluscs: Is modified CTAB DNA extraction method more efficient than DNA extraction kits?
Journal: bioRxiv
doi: 10.1101/863167
Figure Legend Snippet: Comparison of average sequence quality between CTAB and Qiagen® DNeasy Blood and Tissue Kit extracted amplified genomic DNA
Techniques Used: Sequencing, Amplification
Figure Legend Snippet: Chromatogram depicting 16S gene sequence quality in Qiagen ® DNeasy Blood and Tissue Kit extracted DNA.
Techniques Used: Sequencing
9) Product Images from "Absolute Enumeration of Probiotic Strains Lactobacillus acidophilus NCFM® and Bifidobacterium animalis subsp. lactis Bl-04® via Chip-Based Digital PCR"
Article Title: Absolute Enumeration of Probiotic Strains Lactobacillus acidophilus NCFM® and Bifidobacterium animalis subsp. lactis Bl-04® via Chip-Based Digital PCR
Journal: Frontiers in Microbiology
doi: 10.3389/fmicb.2018.00704
Figure Legend Snippet: Qiagen DNeasy replicate isolations of DNA from NCFM overnight cells not treated with PMA. Columns with differing letter are significantly different from each other.
Techniques Used:
10) Product Images from "Development of real-time PCR for detection of Mycoplasma hominis"
Article Title: Development of real-time PCR for detection of Mycoplasma hominis
Journal: BMC Microbiology
doi: 10.1186/1471-2180-4-35
Figure Legend Snippet: DNeasy treated samples. (a) Two LightCycler PCR runs, first one with standard dilution series of human Hep2 DNA before (flat negative curves) and after DNeasy (indicated with squares and triangles), showed on the left and second with DNA free water before (marked with squares) and after DNeasy (triangles) on the right. (b) Melting curve analysis of DNeasy treated H 2 O (triangles), Hep2 DNA (squares) and clinical sample.
Techniques Used: Polymerase Chain Reaction
11) Product Images from "Evaluation of Real-time PCR Based on SYBR Green I Fluorescent Dye for Detection of Bacillus Anthracis Strains in Biological Samples"
Article Title: Evaluation of Real-time PCR Based on SYBR Green I Fluorescent Dye for Detection of Bacillus Anthracis Strains in Biological Samples
Journal: Journal of Veterinary Research
doi: 10.2478/jvetres-2018-0075
Figure Legend Snippet: Determination of the sensitivity of SYBR Green I real-time PCR with rpoB-F/R primers for the detection of B. anthracis strain 1/47 in liver (a) and blood (b) samples using a DNeasy Blood and Tissue Kit for DNA isolation
Techniques Used: SYBR Green Assay, Real-time Polymerase Chain Reaction, DNA Extraction
Figure Legend Snippet: Determination of the sensitivity of SYBR Green I real-time PCR with capC-F/R primers for the detection of B. anthracis strain 1/47 in liver (a) and blood (b) samples using a DNeasy Blood and Tissue Kit for DNA isolation
Techniques Used: SYBR Green Assay, Real-time Polymerase Chain Reaction, DNA Extraction
12) Product Images from "Biocidal Efficacy of Copper Alloys against Pathogenic Enterococci Involves Degradation of Genomic and Plasmid DNAs ▿"
Article Title: Biocidal Efficacy of Copper Alloys against Pathogenic Enterococci Involves Degradation of Genomic and Plasmid DNAs ▿
Journal: Applied and Environmental Microbiology
doi: 10.1128/AEM.03050-09
Figure Legend Snippet: Agarose gel electrophoresis of purified enterococcal DNA. (A) Purified genomic DNA of E. faecium NCTC 12202. Lane 2, cells not exposed to metal surfaces; lane 3, cells exposed to stainless steel for 2 h; lane 4, cells exposed to copper for 2 h; lane 5, cells exposed to copper for 4 h. (B) Purified genomic DNA of clinical isolates E. faecium 5 (lanes 7 and 8) and E. faecalis 2 (lanes 9 and 10) exposed to stainless steel (lanes 7 and 9) or copper (lanes 8 and 10) for 2 h at 22°C. (C) Purified plasmid DNA of E. faecium NCTC 12202 not exposed to metal (lane 11) or exposed to stainless steel (lane 12) or copper (lane 13) for 2 h at 22°C. Control lanes are Bioline Hyperladder I (lanes 1) and Hyperladder II (lanes 6). Genomic DNA was purified using the Qiagen DNeasy Blood and Tissue kit (2% agarose) and the Qiaprep Spin Miniprep kit for plasmid DNA (0.9% agarose).
Techniques Used: Agarose Gel Electrophoresis, Purification, Plasmid Preparation
13) Product Images from "Methods to maximise recovery of environmental DNA from water samples"
Article Title: Methods to maximise recovery of environmental DNA from water samples
Journal: PLoS ONE
doi: 10.1371/journal.pone.0179251
Figure Legend Snippet: Experimental design for Experiment 2A to investigate which DNA extraction kit-filter paper combination would give the best DNA yield. N refers to number of technical replicates. CN, MCE, PES, PCTE and GF refer to the types of filter papers whereas DNeasy and PowerWater (PW) refer to the two DNA extraction kits used in the experiment.
Techniques Used: DNA Extraction
Figure Legend Snippet: DNA yield from eight DNA extraction kit-filter paper combinations from samples in aquaria with UV-sterilized water. Clear bars used DNeasy extraction kit while shaded grey bars used the PowerWater kit. CN = Cellulose Nitrate, MCE = Mixed Cellulose Ester, PES = Polyethersulfone, PCTE = Polycarbonate track-etched. Error bars show the ±2 standard deviation of the mean.
Techniques Used: DNA Extraction, Standard Deviation
Figure Legend Snippet: Experimental design for Experiment 2B investigating DNA yields in five different DNA extraction kit-filter paper combination from stream water. N refers to number of technical replicates. CN, MCE, PES, PCTE and GF refer to the types of filter papers whereas DNeasy and PowerWater (PW) refer to the two DNA extraction kits used in the experiment.
Techniques Used: DNA Extraction
Figure Legend Snippet: Experimental design used to compare five different combinations of capture, preservation and DNA extraction methods. N is the number of technical replicates and each replicate represents one 250 ml water sample. DNeasy and PowerWater (PW) refer to the two DNA extraction kits used in the experiment.
Techniques Used: Preserving, DNA Extraction
Figure Legend Snippet: DNA yield from five different extraction kit-filter paper combinations from stream water samples. Clear bars used DNeasy extraction kit while shaded grey bars used the PowerWater kit. Error bars show the ±2 standard deviation of the mean.
Techniques Used: Standard Deviation
14) Product Images from "Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows, et al. Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows"
Article Title: Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows, et al. Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows
Journal: Ecology and Evolution
doi: 10.1002/ece3.6540
Figure Legend Snippet: Detection rates of the twelve tested workflows for water samples (a) and sediment samples (b), shown is the percentage of positive samples among the 16 PCR reactions of each workflow (CB/ C = CTAB DNA extraction, DN = DNeasy Blood Tissue Kit, HS/ H = high salt DNA extraction, N = Nucleo Spin Soil Kit)
Techniques Used: Polymerase Chain Reaction, DNA Extraction
Figure Legend Snippet: Total DNA yield (a) and threshold cycle values (b) measured in water samples of the twelve tested workflows (CB = CTAB DNA extraction, DN = DNeasy Blood Tissue Kit, HS = high salt DNA extraction). C t values are inversely proportional to the target DNA amount of a sample; thus, the lower the C t value, the higher is the target DNA amount
Techniques Used: DNA Extraction
15) Product Images from "Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows, et al. Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows"
Article Title: Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows, et al. Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows
Journal: Ecology and Evolution
doi: 10.1002/ece3.6540
Figure Legend Snippet: Detection rates of the twelve tested workflows for water samples (a) and sediment samples (b), shown is the percentage of positive samples among the 16 PCR reactions of each workflow (CB/ C = CTAB DNA extraction, DN = DNeasy Blood Tissue Kit, HS/ H = high salt DNA extraction, N = Nucleo Spin Soil Kit)
Techniques Used: Polymerase Chain Reaction, DNA Extraction
Figure Legend Snippet: Total DNA yield (a) and threshold cycle values (b) measured in water samples of the twelve tested workflows (CB = CTAB DNA extraction, DN = DNeasy Blood Tissue Kit, HS = high salt DNA extraction). C t values are inversely proportional to the target DNA amount of a sample; thus, the lower the C t value, the higher is the target DNA amount
Techniques Used: DNA Extraction
16) Product Images from "16S rRNA gene pyrosequencing of reference and clinical samples and investigation of the temperature stability of microbiome profiles"
Article Title: 16S rRNA gene pyrosequencing of reference and clinical samples and investigation of the temperature stability of microbiome profiles
Journal: Microbiome
doi: 10.1186/2049-2618-2-31
Figure Legend Snippet: Variability of oropharyngeal microbiome profiles with storage temperatures. (A) Variability of oropharyngeal microbiome profiles with storage temperatures for the MO BIO PowerSoil extraction method. Four specimens were collected from each subject and stored for 4 weeks at different temperatures. Microbiome profiles at the genus level were estimated using QIIME. The figure presents the principal coordinate analysis based on the weighted UniFrac distances between these microbiome profiles. Each coordinate axis explains the specified percent of the total community variability. (B) Variability of oropharyngeal microbiome profiles with storage temperatures for the Qiagen DNeasy extraction method. Four specimens were collected from each subject and stored for four weeks at different temperatures. Microbiome profiles at the genus level were estimated using QIIME. The figure presents the principal coordinate analysis based on the weighted UniFrac distances between these microbiome profiles. Each coordinate axis explains the specified percent of the total community variability.
Techniques Used:
Figure Legend Snippet: Normalization of PCR amplification by choosing PCR cycle number close to the Ct value. Throat swabs from healthy volunteers were extracted with Qiagen DNeasy (D) or MO BIO PowerSoil (M). DNA extracts were subjected to 16S gene TaqMan qPCR and subsequent PCR using a cycle number of 20, 25, or 30 based on individual sample's qPCR Ct value. PCR amplicons were quantified by PicoGreen dsDNA assay. DNA concentrations for DNA extracts ( blue markers and axis ) and PCR amplicons ( red markers and axis ) are shown in 16S gene copies/μl in the same scale. DNA sample name, 08S1M as an example, depicts the subject number (08), swab number (S1), and extraction method (M).
Techniques Used: Polymerase Chain Reaction, Amplification, Real-time Polymerase Chain Reaction, Picogreen Assay
Figure Legend Snippet: Storage temperature comparisons for Qiagen DNeasy and MO BIO PowerSoil DNA extraction methods for the mock bacterial community HM-280. Identical samples from the mock community were stored at four different temperatures and extracted after 4 weeks using the two methods, Qiagen DNeasy and MO BIO PowerSoil. Microbial profiles at the genus level were estimated using QIIME. Overall, samples were well preserved at lower temperatures for both methods, whereas significant differences were observed at 37°C.
Techniques Used: DNA Extraction
17) Product Images from "16S rRNA gene pyrosequencing of reference and clinical samples and investigation of the temperature stability of microbiome profiles"
Article Title: 16S rRNA gene pyrosequencing of reference and clinical samples and investigation of the temperature stability of microbiome profiles
Journal: Microbiome
doi: 10.1186/2049-2618-2-31
Figure Legend Snippet: Normalization of PCR amplification by choosing PCR cycle number close to the Ct value. Throat swabs from healthy volunteers were extracted with Qiagen DNeasy (D) or MO BIO PowerSoil (M). DNA extracts were subjected to 16S gene TaqMan qPCR and subsequent PCR using a cycle number of 20, 25, or 30 based on individual sample's qPCR Ct value. PCR amplicons were quantified by PicoGreen dsDNA assay. DNA concentrations for DNA extracts ( blue markers and axis ) and PCR amplicons ( red markers and axis ) are shown in 16S gene copies/μl in the same scale. DNA sample name, 08S1M as an example, depicts the subject number (08), swab number (S1), and extraction method (M).
Techniques Used: Polymerase Chain Reaction, Amplification, Real-time Polymerase Chain Reaction, Picogreen Assay
Figure Legend Snippet: Storage temperature comparisons for Qiagen DNeasy and MO BIO PowerSoil DNA extraction methods for the mock bacterial community HM-280. Identical samples from the mock community were stored at four different temperatures and extracted after 4 weeks using the two methods, Qiagen DNeasy and MO BIO PowerSoil. Microbial profiles at the genus level were estimated using QIIME. Overall, samples were well preserved at lower temperatures for both methods, whereas significant differences were observed at 37°C.
Techniques Used: DNA Extraction
18) Product Images from "Impact of Hydrodynamic Injection and phiC31 Integrase on Tumor Latency in a Mouse Model of MYC-Induced Hepatocellular Carcinoma"
Article Title: Impact of Hydrodynamic Injection and phiC31 Integrase on Tumor Latency in a Mouse Model of MYC-Induced Hepatocellular Carcinoma
Journal: PLoS ONE
doi: 10.1371/journal.pone.0011367
![Luciferase activity and PCR analysis of tumors from mice in the pCSI/pLiLucB group provide no evidence of ϕC31 integrase activity. ( a ) Protein extracts were prepared and the luciferase activity was measured in absolute counts per second (CPS). Controls included HeLa cells given FuGene 6 alone [HeLa (-)] or the CMV-luciferase plasmid pNBL2 via FuGene 6 [HeLa (+)], the normal-appearing part of the tumor-ridden liver taken from either a saline-injected mouse [Liver (-)] and pCSI/pLiLucB-injected mouse [Liver (+)]. Eight tumor samples (Tumor 1 through 8) and one metastasis (Met 1) that were obtained from four animals were also analyzed. The error bars give standard error of the mean for four replicates of each sample. ( b ) PCR analysis to detect the pLiLucB plasmid by amplification of the luciferase transgene. Plasmid DNA (20 ng pLiLucB) and no DNA controls show specific amplification of luciferase only in the reaction containing plasmid. One mouse each from the saline-only and pCSmI/pLiLucB groups was analyzed for transgene presence in normal-appearing (N) and tumor (T) tissues (none found). Three mice in the pCSI/pLiLucB group were analyzed for transgene presence in normal-appearing (N) and tumor (T) tissues. Luciferase could be detected in 2/3 normal-appearing liver samples and none of the tumors. ( c ) PCR analysis for integration at the mpsL1 pseudo attP site was done on 18 tumors (lanes 5 through 22) and one metastasis (lane 23) taken from nine mice given pCSI/pLiLucB by hydrodynamic injection. Controls included no DNA (1 st round, lane 1 and 2 nd round, lane 25), and a DNeasy performed on no tissue (lane 2) to show no contamination from the DNA isolation procedure. Normal-appearing liver from a mouse in the pCSI/pLiLucB group (lane 3) served as the positive control. DNA isolated from a tumor in the saline-only group served as the negative control (lane 4). PCR for the GAPDH gene showed that sufficient DNA was added to all reactions. Seven tumors and one metastasis were subjected to the analysis in both a and c . Six tumors were analyzed by all assays ( a , b and c ).](https://storage.googleapis.com/bioz_article_images/PMC2894073/pone.0011367.g003.jpg "... and 2 nd round, lane 25), and a DNeasy performed on no tissue (lane 2) to show ...")
Figure Legend Snippet: Luciferase activity and PCR analysis of tumors from mice in the pCSI/pLiLucB group provide no evidence of ϕC31 integrase activity. ( a ) Protein extracts were prepared and the luciferase activity was measured in absolute counts per second (CPS). Controls included HeLa cells given FuGene 6 alone [HeLa (-)] or the CMV-luciferase plasmid pNBL2 via FuGene 6 [HeLa (+)], the normal-appearing part of the tumor-ridden liver taken from either a saline-injected mouse [Liver (-)] and pCSI/pLiLucB-injected mouse [Liver (+)]. Eight tumor samples (Tumor 1 through 8) and one metastasis (Met 1) that were obtained from four animals were also analyzed. The error bars give standard error of the mean for four replicates of each sample. ( b ) PCR analysis to detect the pLiLucB plasmid by amplification of the luciferase transgene. Plasmid DNA (20 ng pLiLucB) and no DNA controls show specific amplification of luciferase only in the reaction containing plasmid. One mouse each from the saline-only and pCSmI/pLiLucB groups was analyzed for transgene presence in normal-appearing (N) and tumor (T) tissues (none found). Three mice in the pCSI/pLiLucB group were analyzed for transgene presence in normal-appearing (N) and tumor (T) tissues. Luciferase could be detected in 2/3 normal-appearing liver samples and none of the tumors. ( c ) PCR analysis for integration at the mpsL1 pseudo attP site was done on 18 tumors (lanes 5 through 22) and one metastasis (lane 23) taken from nine mice given pCSI/pLiLucB by hydrodynamic injection. Controls included no DNA (1 st round, lane 1 and 2 nd round, lane 25), and a DNeasy performed on no tissue (lane 2) to show no contamination from the DNA isolation procedure. Normal-appearing liver from a mouse in the pCSI/pLiLucB group (lane 3) served as the positive control. DNA isolated from a tumor in the saline-only group served as the negative control (lane 4). PCR for the GAPDH gene showed that sufficient DNA was added to all reactions. Seven tumors and one metastasis were subjected to the analysis in both a and c . Six tumors were analyzed by all assays ( a , b and c ).
Techniques Used: Luciferase, Activity Assay, Polymerase Chain Reaction, Mouse Assay, Plasmid Preparation, Injection, Amplification, DNA Extraction, Positive Control, Isolation, Negative Control
19) Product Images from "Optimizing an eDNA protocol for monitoring endangered Chinook Salmon in the San Francisco Estuary: balancing sensitivity, cost and time"
Article Title: Optimizing an eDNA protocol for monitoring endangered Chinook Salmon in the San Francisco Estuary: balancing sensitivity, cost and time
Journal: bioRxiv
doi: 10.1101/871368
Figure Legend Snippet: Modelled distribution of percentage yield for each extraction protocol. The width of each of the curves shows the variability in modelled yield. The peak of the distribution is the mean yield per extraction type. QIagen DNeasy yields the best yield with little overlap with other methods. Meanwhile magnetic beads and NaOH have shown similar distributions with significant overlap, while both dipstick methods underperform the other methods. It is important to note that this plot does not take into account PCR inhibitor carryover, which might vary significantly between methods.
Techniques Used: Magnetic Beads, Polymerase Chain Reaction
20) Product Images from "Design of a titering assay for lentiviral vectors utilizing direct extraction of DNA from transduced cells in microtiter plates"
Article Title: Design of a titering assay for lentiviral vectors utilizing direct extraction of DNA from transduced cells in microtiter plates
Journal: Molecular Therapy. Methods & Clinical Development
doi: 10.1038/mtm.2016.5
Figure Legend Snippet: Comparison of vector transduction titers measured following in situ versus spin column (DNeasy) DNA extraction. 293T-MLV-DCSIGN assay cells were transduced with equivalent amounts of integration-competent or -deficient vector particles (as measured by vector genomes). Following an overnight incubation, DNA was isolated, and samples were analyzed by qPCR to detect vector sequence. ( a ) Extraction performed using in situ method. Each bar represents the mean ± SD of nine transductions performed in parallel ( n = 3 qPCR replicates/transduction). ( b ) Extraction performed using DNeasy column (5 × 10 4 cells loaded per column). Each bar represents the mean ± SD of qPCR triplicates for a single transduction; two transductions were performed in parallel for each vector tested (replicates 1, 2). Particle-to-infectivity (P:I) ratios were calculated by dividing the physical particle titer (measured by RNA genome content per vector) by the measured transduction titer. Results in both panels are representative of two independent experiments. TU, transduction units.
Techniques Used: Plasmid Preparation, Transduction, In Situ, DNA Extraction, Incubation, Isolation, Real-time Polymerase Chain Reaction, Sequencing, Infection
21) Product Images from "Development of Loop-Mediated Isothermal Amplification Targeting 18S Ribosomal DNA for Rapid Detection of Azumiobodo hoyamushi (Kinetoplastea)"
Article Title: Development of Loop-Mediated Isothermal Amplification Targeting 18S Ribosomal DNA for Rapid Detection of Azumiobodo hoyamushi (Kinetoplastea)
Journal: The Korean Journal of Parasitology
doi: 10.3347/kjp.2014.52.3.305
![Detection limit of Azumiobodo hoyamushi 18S rDNA LAMP assays (A). LAMP assays were performed using serial dilutions of A. hoyamushi genomic DNA (1 ng to 1 fg per reaction). Distilled water was used as a negative control. LAMP products were visualized by gel electrophoresis (B) and using Loopamp® fluorescent detection reagent (FD) (C). (B, C) Lane M, 100-bp DNA marker; lane 1, 1 ng; lane 2, 100 pg; lane 3, 10 pg; lane 4, 1 pg; lane 5, 100 fg; lane 6, 10 fg; lane 7, 1 fg of A. hoyamushi genomic DNA; lane 8, distilled water; and lane 9, LAMP product after Mbo I digestion. (D-E) A. hoyamushi at a density of 1×10 3 parasites/µl was serially diluted and tested (D) using the LAMP assay (D) and by PCR (E) using F3 and B3 primers. Lane M, 100-bp DNA marker; lane 1, 1,000; lane 2, 100; lane 3, 10; lane 4, 1; lane 5, 0.1; lane 6, 0.01 of parasites per reaction; lane 7, distilled water. A. hoyamushi genomic DNA was prepared using DNeasy tissue kits (Qiagen) from in vitro cultured A. hoyamushi species [ 9 ] which were kindly provided by Dr. Kyung Il Park (Kunsan National University, Gunsan, Korea).](https://storage.googleapis.com/bioz_article_images/PMC4096644/kjp-52-305-g001.jpg "... water. A. hoyamushi genomic DNA was prepared using DNeasy tissue kits (Qiagen) from in vitro cultured A. ...")
Figure Legend Snippet: Detection limit of Azumiobodo hoyamushi 18S rDNA LAMP assays (A). LAMP assays were performed using serial dilutions of A. hoyamushi genomic DNA (1 ng to 1 fg per reaction). Distilled water was used as a negative control. LAMP products were visualized by gel electrophoresis (B) and using Loopamp® fluorescent detection reagent (FD) (C). (B, C) Lane M, 100-bp DNA marker; lane 1, 1 ng; lane 2, 100 pg; lane 3, 10 pg; lane 4, 1 pg; lane 5, 100 fg; lane 6, 10 fg; lane 7, 1 fg of A. hoyamushi genomic DNA; lane 8, distilled water; and lane 9, LAMP product after Mbo I digestion. (D-E) A. hoyamushi at a density of 1×10 3 parasites/µl was serially diluted and tested (D) using the LAMP assay (D) and by PCR (E) using F3 and B3 primers. Lane M, 100-bp DNA marker; lane 1, 1,000; lane 2, 100; lane 3, 10; lane 4, 1; lane 5, 0.1; lane 6, 0.01 of parasites per reaction; lane 7, distilled water. A. hoyamushi genomic DNA was prepared using DNeasy tissue kits (Qiagen) from in vitro cultured A. hoyamushi species [ 9 ] which were kindly provided by Dr. Kyung Il Park (Kunsan National University, Gunsan, Korea).
Techniques Used: Negative Control, Nucleic Acid Electrophoresis, Marker, Lamp Assay, Polymerase Chain Reaction, In Vitro, Cell Culture
22) Product Images from "Conventional and PCR Detection of Aphelenchoides fragariae in Diverse Ornamental Host Plant Species"
Article Title: Conventional and PCR Detection of Aphelenchoides fragariae in Diverse Ornamental Host Plant Species
Journal:
doi:
Figure Legend Snippet: Comparison of DNA extraction methods for detection of Aphelenchoides fragariae in naturally infected Asplenium nidus (Bird's nest fern) plant tissue using species-specific PCR primers. Lane 1: healthy A. nidus extracted with Qiagen Dneasy Plant Mini Kit;
Techniques Used: DNA Extraction, Infection, Polymerase Chain Reaction
23) Product Images from "Loop-mediated isothermal amplification (LAMP) assay—A rapid detection tool for identifying red fox (Vulpes vulpes) DNA in the carcasses of harbour porpoises (Phocoena phocoena)"
Article Title: Loop-mediated isothermal amplification (LAMP) assay—A rapid detection tool for identifying red fox (Vulpes vulpes) DNA in the carcasses of harbour porpoises (Phocoena phocoena)
Journal: PLoS ONE
doi: 10.1371/journal.pone.0184349
Figure Legend Snippet: Application of the LAMP assay on a stranded harbour porpoise. Gel electrophoresis of all LAMP products of the stranded harbour porpoise. Directly tested MSwab ™ samples (without DNA isolation) and DNA isolation of the MSwab ™ medium using DNeasy ® blood and tissue kit.
Techniques Used: Lamp Assay, Nucleic Acid Electrophoresis, DNA Extraction
24) Product Images from "Isolation and preservation of schistosome eggs and larvae in RNAlater® facilitates genetic profiling of individuals"
Article Title: Isolation and preservation of schistosome eggs and larvae in RNAlater® facilitates genetic profiling of individuals
Journal: Parasites & Vectors
doi: 10.1186/1756-3305-2-50
Figure Legend Snippet: Schematic of the RNA later ® preservation and gDNA extraction of individual schistosome larval stages and eggs . ATL and AL are lysis buffers supplied in the Qiagen DNeasy Blood and Tissue Kit. *These were the longest times and highest temperatures tested but it is expected that the samples can be preserved for much longer.
Techniques Used: Preserving, Lysis
25) Product Images from "Species‐level biodiversity assessment using marine environmental DNA metabarcoding requires protocol optimization and standardization, et al. Species‐level biodiversity assessment using marine environmental DNA metabarcoding requires protocol optimization and standardization"
Article Title: Species‐level biodiversity assessment using marine environmental DNA metabarcoding requires protocol optimization and standardization, et al. Species‐level biodiversity assessment using marine environmental DNA metabarcoding requires protocol optimization and standardization
Journal: Ecology and Evolution
doi: 10.1002/ece3.4843
Figure Legend Snippet: DNA yield from seven extraction protocols (DNeasy: Qiagen DNeasy Blood Tissue Kit; MO BIO PW: MO BIO PowerWater DNA Isolation Kit; MO BIO PMS: MO BIO PowerMax Soil DNA Isolation Kit; Dnature: Presto™ Mini gDNA Bacteria Kit; PCI: phenol‐chloroform‐isoamyl extraction procedure; Silica: Silica extraction procedure; and Magnetic beads: Magnetic beads extraction procedure) obtained from 1,000 ml technical replicates filtered through 1.2‐µm cellulose‐nitrate filters. Error bars show 95% confidence intervals. Letters indicate significant differences among groups based on Tukey–Kramer's test (α = 0.05)
Techniques Used: DNA Extraction, Magnetic Beads
Figure Legend Snippet: The average (a) OTU and (b) taxon richness obtained per replicate for each of the four assays between the optimal (blue; 1.2‐µm cellulose‐nitrate filter and Qiagen's DNeasy Blood Tissue Kit) and low‐performance (gold; 1.2‐µm polycarbonate filter and MO BIO's PowerMax Soil) protocol. Error bars show 95% confidence intervals. T test significance is depicted by: *** p
Techniques Used:
Figure Legend Snippet: Observed taxa in each of the four assays. Filled and unfilled rectangles indicate taxon presence or absence. Taxa show a pair of rectangles per assay, representing the low‐performance (PCPMS; 1.2‐µm polycarbonate filter and MO BIO's PowerMax Soil) and the optimal (CNQ; 1.2‐µm cellulose‐nitrate filter and Qiagen's DNeasy Blood Tissue Kit) treatment. Rectangular presence indicates the amplification range of each assay
Techniques Used: Amplification
26) Product Images from "Comparison of DNA extraction methods for non-marine molluscs: is modified CTAB DNA extraction method more efficient than DNA extraction kits?"
Article Title: Comparison of DNA extraction methods for non-marine molluscs: is modified CTAB DNA extraction method more efficient than DNA extraction kits?
Journal: 3 Biotech
doi: 10.1007/s13205-020-2051-7
Figure Legend Snippet: Chromatogram depicting 16S gene sequence quality in Qiagen ® DNeasy Blood and Tissue Kit extracted DNA
Techniques Used: Sequencing
27) Product Images from "Comparing Two Common DNA Extraction Kits for the Characterization of Symbiotic Microbial Communities from Ascidian Tissue"
Article Title: Comparing Two Common DNA Extraction Kits for the Characterization of Symbiotic Microbial Communities from Ascidian Tissue
Journal: Microbes and Environments
doi: 10.1264/jsme2.ME18031
Figure Legend Snippet: Non-metric multi-dimensional scaling plot based on the Bray-Curtis similarity of microbial communities obtained using DNeasy (black triangles) and PowerSoil (gray circles) kits. Circles encompass samples clustering at > 30% similarity and correspond to replicates of Clavelina oblonga (dashed line) and Polyandrocarpa anguinea (solid line), indicating a high degree of host-specificity and low technical variation across DNA extraction methods.
Techniques Used: DNA Extraction
Figure Legend Snippet: Microbial community composition averaged by source and kit (a), and for each replicate sample within each source: Clavelina oblonga , DNeasy (b), C. oblonga , PowerSoil (c), Polyandrocarpa anguinea , DNeasy (d), and P. anguinea , PowerSoil (e). Phylum-level classifications are shown, except for Proteobacteria , which were divided into four classes: Alpha -, Gamma -, Delta -, and Epsilonproteobacteria . Each library represents 20,007 sequence reads.
Techniques Used: Sequencing
28) Product Images from "Demonstration of the Use of Environmental DNA for the Non-Invasive Genotyping of a Bivalve Mollusk, the European Flat Oyster (Ostrea edulis)"
Article Title: Demonstration of the Use of Environmental DNA for the Non-Invasive Genotyping of a Bivalve Mollusk, the European Flat Oyster (Ostrea edulis)
Journal: Frontiers in Genetics
doi: 10.3389/fgene.2019.01159
Figure Legend Snippet: Diagram (top) detailing dilutions of DNA template from different DNA extraction techniques used for SNP genotyping of environmental DNA for Ostrea edulis . The two DNA extraction methods, the Qiagen DNeasy Blood and Tissue Kit and the crude Chelex extraction, are shown at the top. DNA samples are subject to dilution step before and after the Specific Target Amplification PCR (STA PCR), here shown as a blue bar. All dilutions were with PCR grade water and samples that were not diluted are labelled ‘neat’. A scatter plot (bottom) shows the percentage of correct environmental DNA derived genotype calls in comparison to the tissue extractions for the eight dilutions. Each point represents the total result derived from a random sample of three replicates per individual oyster genotype. The black line indicates the average percentage correct eDNA genotypes across the 100 random samples. The red line indicates the average percentage correct eDNA genotypes across the 100 samples if only a single replicate is used for each individual oyster genotype.
Techniques Used: DNA Extraction, Amplification, Polymerase Chain Reaction, Derivative Assay
29) Product Images from "Comparison of four DNA extraction and three preservation protocols for the molecular detection and quantification of soil-transmitted helminths in stool"
Article Title: Comparison of four DNA extraction and three preservation protocols for the molecular detection and quantification of soil-transmitted helminths in stool
Journal: PLoS Neglected Tropical Diseases
doi: 10.1371/journal.pntd.0007778
Figure Legend Snippet: The sensitivity and DNA concentration across four DNA extraction protocols for Trichuris and Necator . The left panel represents the sensitivity (sen) and geometric mean of DNA concentration expressed as genome equivalents per ml of DNA extract (mean; GE/ml) for 20 Necator americanus samples preserved in 96% ethanol and extracted by four DNA extraction protocols. The DNA extraction protocols include the QIAamp DNA Stool Mini kit without (SK) and with bead beating (SK + BB), DNeasy Blood Tissue kit without (TK) and without bead beating (TK + BB). The right panel represents the same parameters across 36 Trichuris trichiura samples preserved in 96% ethanol. Each line represents a sample.
Techniques Used: Concentration Assay, DNA Extraction
30) Product Images from "Comparison of four DNA extraction and three preservation protocols for the molecular detection and quantification of soil-transmitted helminths in stool"
Article Title: Comparison of four DNA extraction and three preservation protocols for the molecular detection and quantification of soil-transmitted helminths in stool
Journal: PLoS Neglected Tropical Diseases
doi: 10.1371/journal.pntd.0007778
Figure Legend Snippet: The sensitivity and DNA concentration across four DNA extraction protocols for Trichuris and Necator . The left panel represents the sensitivity (sen) and geometric mean of DNA concentration expressed as genome equivalents per ml of DNA extract (mean; GE/ml) for 20 Necator americanus samples preserved in 96% ethanol and extracted by four DNA extraction protocols. The DNA extraction protocols include the QIAamp DNA Stool Mini kit without (SK) and with bead beating (SK + BB), DNeasy Blood Tissue kit without (TK) and without bead beating (TK + BB). The right panel represents the same parameters across 36 Trichuris trichiura samples preserved in 96% ethanol. Each line represents a sample.
Techniques Used: Concentration Assay, DNA Extraction
31) Product Images from "Evaluation of rapid extraction and isothermal amplification techniques for the detection of Leishmania donovani DNA from skin lesions of suspected cases at the point of need in Sri Lanka"
Article Title: Evaluation of rapid extraction and isothermal amplification techniques for the detection of Leishmania donovani DNA from skin lesions of suspected cases at the point of need in Sri Lanka
Journal: Parasites & Vectors
doi: 10.1186/s13071-018-3238-1
Figure Legend Snippet: Comparison between the sample types and test methods. The slit skin smears were examined with microscopy. The skin scrapping and aspirate were tested at the point of need with SpeedXtract (SE) and recombinase polymerase amplification (RPA) assay. The punch biopsies were screened with SE and RPA as well as DNeasy blood and tissue kit (mini) and polymerase chain reaction (PCR). Abbreviations : ID, sample identification code; POS, Leishmania donovani was detected; NEG, negative test results
Techniques Used: Microscopy, Recombinase Polymerase Amplification, Polymerase Chain Reaction
32) Product Images from "A vicious loop of fatty acid-binding protein 4 and DNA methyltransferase 1 promotes acute myeloid leukemia and acts as a therapeutic target"
Article Title: A vicious loop of fatty acid-binding protein 4 and DNA methyltransferase 1 promotes acute myeloid leukemia and acts as a therapeutic target
Journal: Leukemia
doi: 10.1038/leu.2017.307
Figure Legend Snippet: Pharmacological inhibition of FABP4 induces DNA hypomethylation and restores the epigenetically silenced p15 INK4N . ( a and b ) Western blotting of C1498, MV4-11 or Kasumi-1 cells exposed to BMS. ( c ) C1498, MV4-11 or Kasumi-1 cells were treated with BMS and the genomic DNA was subjected to Dotblotting. Left, representative images of dots; right, graph shows quantification of dot intensity. ( d ) qPCR of C1498, MV4-11 or Kasumi-1 cells treated with BMS. ( e ) Bisulfite sequencing of the p15 INK4B promoter in MV4-11 or Kasumi-1 cells treated with BMS. Results of 10 clones are presented. Vertical bars indicate CpG locations, arrows indicate the bisulfite sequencing region, open circles show unmethylated CpG, and solid circles show methylated CpGs. ( f ) qPCR of human patient (n = 8) or mouse (n = 3) AML primary cells treated with BMS. The experiments were performed three times independently and data are shown as mean values ± SD, * P
Techniques Used: Inhibition, Western Blot, Real-time Polymerase Chain Reaction, Methylation Sequencing, Clone Assay, Methylation
33) Product Images from "Development of real-time PCR for detection of Mycoplasma hominis"
Article Title: Development of real-time PCR for detection of Mycoplasma hominis
Journal: BMC Microbiology
doi: 10.1186/1471-2180-4-35
Figure Legend Snippet: DNeasy treated samples. (a) Two LightCycler PCR runs, first one with standard dilution series of human Hep2 DNA before (flat negative curves) and after DNeasy (indicated with squares and triangles), showed on the left and second with DNA free water before (marked with squares) and after DNeasy (triangles) on the right. (b) Melting curve analysis of DNeasy treated H 2 O (triangles), Hep2 DNA (squares) and clinical sample.
Techniques Used: Polymerase Chain Reaction
34) Product Images from "Tissue Fractionation by Hydrostatic Pressure Cycling Technology: The Unified Sample Preparation Technique for Systems Biology Studies"
Article Title: Tissue Fractionation by Hydrostatic Pressure Cycling Technology: The Unified Sample Preparation Technique for Systems Biology Studies
Journal: Journal of Biomolecular Techniques : JBT
doi:
Figure Legend Snippet: A: DNA and protein recovery from the cell culture. one aliquot of cells was processed with PCT and ProteoSolve-SB (P). DNA was extracted from the solid phase using the DNeasy kit. The control aliquot of cells was processed directly with the qiagen DNeasy
Techniques Used: Cell Culture
35) Product Images from "Dissemination of Orientia tsutsugamushi, a Causative Agent of Scrub Typhus, and Immunological Responses in the Humanized DRAGA Mouse"
Article Title: Dissemination of Orientia tsutsugamushi, a Causative Agent of Scrub Typhus, and Immunological Responses in the Humanized DRAGA Mouse
Journal: Frontiers in Immunology
doi: 10.3389/fimmu.2018.00816
Figure Legend Snippet: Live Orientia tsutsugamushi disseminates into organs of infected humanized DRAGA mice. (A) DNA was extracted from various organs of humanized DRAGA mice 2 weeks post infection at 6 × 10 4 mLD 50 and O. tsutsugamushi was quantified by quantitative PCR (qPCR). (B) Immunofluorescence staining showing O. tsutsugamushi (red) in lung tissue frozen sections from humanized DRAGA mice 3 weeks post infection at 6 × 10 2 mLD 50 (cell nuclei were stained blue with DAPI, magnification, 400×). (C) Tissue homogenates were prepared from lung tissue and used to inoculate L929 cells. Immunofluorescence staining was performed to detect the presence of O. tsutsugamushi (magnification, 400×) at day 7 and day 14 post inoculation. (D) qPCR was used to quantify number of Orientia in L929 cells collected in (C) .
Techniques Used: Infection, Mouse Assay, Real-time Polymerase Chain Reaction, Immunofluorescence, Staining
36) Product Images from "Silica particles contribute to the procoagulant activity of DNA and polyphosphate isolated using commercial kits"
Article Title: Silica particles contribute to the procoagulant activity of DNA and polyphosphate isolated using commercial kits
Journal: Blood
doi: 10.1182/blood-2017-03-772848
Figure Legend Snippet: Procoagulant activities of DNA, polyP, and silica particles. (A-D) Shown are plasma clot times versus concentration, with horizontal dashed lines showing the mean clot time without activator (± standard error of the mean as horizontal dotted lines). (A) Clot times with: HEK 293 cell DNA isolated with DNeasy Blood Tissue (open diamond); HEK 293 cell DNA isolated with phenol/chloroform (solid diamond); NET-derived DNA isolated with DNeasy Blood Tissue kit (open inverted triangle); or NET-derived DNA isolated with phenol/chloroform (solid inverted triangle). Each data set represents the mean clot time for 3 separate purifications as detailed in supplemental Figure 2A (HEK293 cell DNA) and supplemental Figure 2B (NET DNA). (B) Clot times with silica particles: glass milk (solid triangle) or homogenized DNeasy column matrix (open triangle). (C) Clot times with water elutions from 3 different lots of DNeasy columns (open triangle) or 2 different lots of Econospin columns (open circle). On the x-axis, fold concentration refers to the concentration relative to the volume eluted from the Qiagen column, with 1 equaling the original elution volume. (D) Clot times of λ phage DNA before (blue square) or after (open square) repurification on DNeasy columns; or of short-chain polyP before (blue circle) or after (open circle) repurification on DNeasy columns. The purification data sets represent mean clot times for 2 different purifications as detailed in supplemental Figure 4. (E) Clot times of various samples before (red bars) or after (blue bars) digestion with a combination of Benzonase and calf intestine alkaline phosphatase. Samples include buffer control (no activator), 20 µg/mL HEK 293 cell DNA purified using DNeasy, 20 µg/mL NET DNA purified using DNeasy, water applied to DNeasy column as described in panel C (water elution), or 2 µg/mL long-chain polyP. Digestion of DNA by Benzonase and polyP by phosphatase was confirmed by gel electrophoresis (supplemental Figure 1). (F) Clot times of various samples before (red bars) or after (blue bars) acid hydrolysis. Samples include buffer control (no activator), 20 µg/mL HEK 293 cell DNA purified using DNeasy, 20 µg/mL NET DNA purified using DNeasy, water applied to DNeasy column (water elution), 2 µg/mL long-chain polyP, or 5 µg/mL polyguanylate (polyG). (G) Clot times in the presence of varying concentrations of histones with (open triangle) or without (solid triangle) a 10-fold concentrated water elution (prepared as described for panel C). (H) Tissue factor–triggered clot times in the presence of varying concentrations of histones.
Techniques Used: Concentration Assay, Isolation, Derivative Assay, Purification, Nucleic Acid Electrophoresis
37) Product Images from "Development of Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid and Sensitive Identification of Ostrich Meat"
Article Title: Development of Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid and Sensitive Identification of Ostrich Meat
Journal: PLoS ONE
doi: 10.1371/journal.pone.0100717
Figure Legend Snippet: Amplification signal of ostrich meat DNA using the direct swab and HYPLEX buffer test method. The red curve is the signal of ostrich DNA isolated with DNeasy tissue Isolation Kit (detection time of 6∶18 min). The other positive curves in the oval shap are the direct swab samples with a detection time of 11∶03–12∶18 min. The pink curve is the negative control (see table 3 ).
Techniques Used: Amplification, Isolation, Negative Control
38) Product Images from "Cholestyramine as a Promising, Strong Anion Exchange Resin for Direct Capture of Genetic Biomarkers from Raw Pancreatic Fluids"
Article Title: Cholestyramine as a Promising, Strong Anion Exchange Resin for Direct Capture of Genetic Biomarkers from Raw Pancreatic Fluids
Journal: Biotechnology and bioengineering
doi: 10.1002/bit.26207
Figure Legend Snippet: (a) Recovered DNA quantities using resin-based capture from endoscopically collected pancreatic fluid samples from 6 patients with suspected chronic pancreatitis at three different time points. (b) Parity plot comparing the amount of DNA recovered using the resin capture approach and DNeasy spin columns. Cholestyramine resin demonstrates a similar performance to the DNeasy spin columns, which represent the state-of-the-art technology for in vitro DNA capture from biological fluids. Samples were processed in duplicate, and error bars represent +/− 1 SD. DNA concentrations were evaluated using the Quantifiler Human DNA Quantification Kit.
Techniques Used: In Vitro
39) Product Images from "Species‐level biodiversity assessment using marine environmental DNA metabarcoding requires protocol optimization and standardization, et al. Species‐level biodiversity assessment using marine environmental DNA metabarcoding requires protocol optimization and standardization"
Article Title: Species‐level biodiversity assessment using marine environmental DNA metabarcoding requires protocol optimization and standardization, et al. Species‐level biodiversity assessment using marine environmental DNA metabarcoding requires protocol optimization and standardization
Journal: Ecology and Evolution
doi: 10.1002/ece3.4843
Figure Legend Snippet: DNA yield from seven extraction protocols (DNeasy: Qiagen DNeasy Blood Tissue Kit; MO BIO PW: MO BIO PowerWater DNA Isolation Kit; MO BIO PMS: MO BIO PowerMax Soil DNA Isolation Kit; Dnature: Presto™ Mini gDNA Bacteria Kit; PCI: phenol‐chloroform‐isoamyl extraction procedure; Silica: Silica extraction procedure; and Magnetic beads: Magnetic beads extraction procedure) obtained from 1,000 ml technical replicates filtered through 1.2‐µm cellulose‐nitrate filters. Error bars show 95% confidence intervals. Letters indicate significant differences among groups based on Tukey–Kramer's test (α = 0.05)
Techniques Used: DNA Extraction, Magnetic Beads
Figure Legend Snippet: The average (a) OTU and (b) taxon richness obtained per replicate for each of the four assays between the optimal (blue; 1.2‐µm cellulose‐nitrate filter and Qiagen's DNeasy Blood Tissue Kit) and low‐performance (gold; 1.2‐µm polycarbonate filter and MO BIO's PowerMax Soil) protocol. Error bars show 95% confidence intervals. T test significance is depicted by: *** p
Techniques Used:
Figure Legend Snippet: Observed taxa in each of the four assays. Filled and unfilled rectangles indicate taxon presence or absence. Taxa show a pair of rectangles per assay, representing the low‐performance (PCPMS; 1.2‐µm polycarbonate filter and MO BIO's PowerMax Soil) and the optimal (CNQ; 1.2‐µm cellulose‐nitrate filter and Qiagen's DNeasy Blood Tissue Kit) treatment. Rectangular presence indicates the amplification range of each assay
Techniques Used: Amplification
40) Product Images from "Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows, et al. Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows"
Article Title: Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows, et al. Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows
Journal: Ecology and Evolution
doi: 10.1002/ece3.6540
Figure Legend Snippet: Detection rates of the twelve tested workflows for water samples (a) and sediment samples (b), shown is the percentage of positive samples among the 16 PCR reactions of each workflow (CB/ C = CTAB DNA extraction, DN = DNeasy Blood Tissue Kit, HS/ H = high salt DNA extraction, N = Nucleo Spin Soil Kit)
Techniques Used: Polymerase Chain Reaction, DNA Extraction
Figure Legend Snippet: Total DNA yield (a) and threshold cycle values (b) measured in water samples of the twelve tested workflows (CB = CTAB DNA extraction, DN = DNeasy Blood Tissue Kit, HS = high salt DNA extraction). C t values are inversely proportional to the target DNA amount of a sample; thus, the lower the C t value, the higher is the target DNA amount
Techniques Used: DNA Extraction
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