p quinata  (Qiagen)


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    Name:
    DNeasy 96 Plant Kit
    Description:
    For isolation of up to 15 µg per well total cellular DNA from plant tissue Kit contents Qiagen DNeasy 96 Plant Kit 6 x 96 preps 50mg Sample 100 to 200L Elution Volume Plant Cells Tissues Sample DNA Purification Silica Technology Manual Processing 96 well Plate Format 1 to 15g Yield Ideal for PCR Real time PCR Blotting For Isolation of Up to 15g Well Total Cellular DNA from Plant Tissue Includes 6 DNeasy 96 Plates Buffers Reagents RNase A S Blocks 1 2mL Collection Microtubes Caps AirPore Tape Sheets Benefits Pure DNA free from contaminants and enzyme inhibitors Rapid purification of ready to use DNA No organic extraction no ethanol precipitatio
    Catalog Number:
    69181
    Price:
    1739
    Category:
    DNeasy 96 Plant Kit
    Buy from Supplier


    Structured Review

    Qiagen p quinata
    DNeasy 96 Plant Kit
    For isolation of up to 15 µg per well total cellular DNA from plant tissue Kit contents Qiagen DNeasy 96 Plant Kit 6 x 96 preps 50mg Sample 100 to 200L Elution Volume Plant Cells Tissues Sample DNA Purification Silica Technology Manual Processing 96 well Plate Format 1 to 15g Yield Ideal for PCR Real time PCR Blotting For Isolation of Up to 15g Well Total Cellular DNA from Plant Tissue Includes 6 DNeasy 96 Plates Buffers Reagents RNase A S Blocks 1 2mL Collection Microtubes Caps AirPore Tape Sheets Benefits Pure DNA free from contaminants and enzyme inhibitors Rapid purification of ready to use DNA No organic extraction no ethanol precipitatio
    https://www.bioz.com/result/p quinata/product/Qiagen
    Average 85 stars, based on 52 article reviews
    Price from $9.99 to $1999.99
    p quinata - by Bioz Stars, 2020-04
    85/100 stars

    Images

    1) Product Images from "Aquaporins in the wild: natural genetic diversity and selective pressure in the PIP gene family in five Neotropical tree species"

    Article Title: Aquaporins in the wild: natural genetic diversity and selective pressure in the PIP gene family in five Neotropical tree species

    Journal: BMC Evolutionary Biology

    doi: 10.1186/1471-2148-10-202

    Graphical representation of observed statistics and their neutral confidence intervals for each of the analysed genes . (a) Tajima's D statistic; (b) Fu and Li's F * statistic; (c) Fu and Li's D * statistic; (d) Fu's F S statistic; (e) Nei's genetic diversity H . A, Carapa guianensis population SE; B, Carapa guianensis population NW; C, Eperua falcata , population NW; D, Eperua falcata , population SW; E, Eperua grandiflora ; F, Virola sebifera ; G, Pachira quinata . For each species and population, genes are the same as in Table 3 . For C and D, genes appear in the order: EfaPIP1.1, EfaPIP1.2, EfaPIP2.1. Filled squares: observed values for each test (red, green = significant, P
    Figure Legend Snippet: Graphical representation of observed statistics and their neutral confidence intervals for each of the analysed genes . (a) Tajima's D statistic; (b) Fu and Li's F * statistic; (c) Fu and Li's D * statistic; (d) Fu's F S statistic; (e) Nei's genetic diversity H . A, Carapa guianensis population SE; B, Carapa guianensis population NW; C, Eperua falcata , population NW; D, Eperua falcata , population SW; E, Eperua grandiflora ; F, Virola sebifera ; G, Pachira quinata . For each species and population, genes are the same as in Table 3 . For C and D, genes appear in the order: EfaPIP1.1, EfaPIP1.2, EfaPIP2.1. Filled squares: observed values for each test (red, green = significant, P

    Techniques Used:

    Related Articles

    DNA Extraction:

    Article Title: Commercial Teas Highlight Plant DNA Barcode Identification Successes and Obstacles
    Article Snippet: .. DNA extraction and sequencing DNA was isolated from 5–15 mg dried tissue using a DNeasy96 Plant kit (Qiagen). .. The manufacturer's protocol was modified as follows: tissue was disrupted and then incubated for 12–18 h with gentle mixing at 42°C in 600 µL of the supplied AP1 buffer with 600 µg of protease K added (630 µL total volume).

    Article Title: Local adaptation (mostly) remains local: reassessing environmental associations of climate-related candidate SNPs in Arabidopsis halleri
    Article Snippet: Paragraph title: Sampling and DNA extraction ... Sampled leaf tissue was dried in silica gel, and DNA was extracted with the Qiagen DNeasy Plant Mini Kit or DNeasy 96 Plant Kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol.

    Article Title: Multiple Multilocus DNA Barcodes from the Plastid Genome Discriminate Plant Species Equally Well
    Article Snippet: .. DNA isolation and amplification For each sample, we isolated total genomic DNA from approximately 10 mg of dried leaf material using DNeasy 96 Plant Kits (QIAGEN) following manufacturer instructions. .. We performed DNA amplification with various annealing temperatures depending on the primers used ( ).

    Article Title: Anthropogenic disturbance as a driver of microspatial and microhabitat segregation of cytotypes of Centaurea stoebe and cytotype interactions in secondary contact zones
    Article Snippet: Paragraph title: DNA extraction and molecular analyses ... DNA was extracted from 10–15 mg of silica-dried leaf tissue with a DNeasy 96 Plant Kit and DNeasy Plant Mini Kit (Qiagen) following the manufacturer's protocol.

    Article Title: Allantoin accumulation through overexpression of ureide permease1 improves rice growth under limited nitrogen conditions
    Article Snippet: .. For genotyping, genomic DNA extraction was performed using DNeasy 96 Plant Kit (QIAGEN, Valencia, CA), according to manufacturer's instructions. .. T‐DNA insertion was then confirmed through PCR using the primers listed in Table .

    Clone Assay:

    Article Title: Local adaptation (mostly) remains local: reassessing environmental associations of climate-related candidate SNPs in Arabidopsis halleri
    Article Snippet: We collected 20 individuals per population and maintained a minimal distance of 2 m (preferably 4 m) among plants to avoid potential clones. .. Sampled leaf tissue was dried in silica gel, and DNA was extracted with the Qiagen DNeasy Plant Mini Kit or DNeasy 96 Plant Kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol.

    Amplification:

    Article Title: Host ecotype generates evolutionary and epidemiological divergence across a pathogen metapopulation
    Article Snippet: Genomic DNA was extracted from 100 mg of spores per isolate using a DNeasy 96 plant kit (QIAGEN). .. For AvrP4 , a 568 bp PCR product was amplified using 5′-CATCAAAATCTAACCCGTAC (forward) and 5′-GCACTATCTATCCTGAACAA (reverse) primer pair.

    Article Title: Multiple Multilocus DNA Barcodes from the Plastid Genome Discriminate Plant Species Equally Well
    Article Snippet: .. DNA isolation and amplification For each sample, we isolated total genomic DNA from approximately 10 mg of dried leaf material using DNeasy 96 Plant Kits (QIAGEN) following manufacturer instructions. .. We performed DNA amplification with various annealing temperatures depending on the primers used ( ).

    Article Title: Allantoin accumulation through overexpression of ureide permease1 improves rice growth under limited nitrogen conditions
    Article Snippet: Plant material, genotyping and rice transformation T‐DNA insertion line (PFG_2D‐4064.L) was provided by Prof. Gynheung An of Kyunghee University and amplified on a rice paddy field. .. For genotyping, genomic DNA extraction was performed using DNeasy 96 Plant Kit (QIAGEN, Valencia, CA), according to manufacturer's instructions.

    Polymerase Chain Reaction:

    Article Title: Host ecotype generates evolutionary and epidemiological divergence across a pathogen metapopulation
    Article Snippet: Genomic DNA was extracted from 100 mg of spores per isolate using a DNeasy 96 plant kit (QIAGEN). .. For AvrP4 , a 568 bp PCR product was amplified using 5′-CATCAAAATCTAACCCGTAC (forward) and 5′-GCACTATCTATCCTGAACAA (reverse) primer pair.

    Article Title: Anthropogenic disturbance as a driver of microspatial and microhabitat segregation of cytotypes of Centaurea stoebe and cytotype interactions in secondary contact zones
    Article Snippet: DNA was extracted from 10–15 mg of silica-dried leaf tissue with a DNeasy 96 Plant Kit and DNeasy Plant Mini Kit (Qiagen) following the manufacturer's protocol. .. Amplifications were performed in a 25-μL volume containing 5 µL genomic DNA (4 ng μL−1 ), 2·5 µ m buffer (10× PCR buffer), 1 m m MgCl2 , 0·2 m m of each dNTP, 0·4 µ m of each primer, 0·25 µ m bovine serum albumin (BSA) and 1 U Taq polymerase (Qiagen).

    Article Title: The Effect of Plant Genotype, Growth Stage, and Mycosphaerella graminicola Strains on the Efficiency and Durability of Wheat-Induced Resistance by Paenibacillus sp. Strain B2
    Article Snippet: DNA was extracted from the plant roots using DNeasy 96 Plant kit (Qiagen, United States), according to the manufacturer’s protocol. .. SYBR Green qPCR assays were carried out in a reaction mixture of 25 μL that contained the following: 12.5 μl Universal Quantifast SYBR Green PCR master mix (Qiagen, United States), 0.3 μM of each primer, 50 ng of DNA, and water up to a volume of 25 μl.

    Article Title: Allantoin accumulation through overexpression of ureide permease1 improves rice growth under limited nitrogen conditions
    Article Snippet: For genotyping, genomic DNA extraction was performed using DNeasy 96 Plant Kit (QIAGEN, Valencia, CA), according to manufacturer's instructions. .. T‐DNA insertion was then confirmed through PCR using the primers listed in Table .

    Isolation:

    Article Title: Commercial Teas Highlight Plant DNA Barcode Identification Successes and Obstacles
    Article Snippet: .. DNA extraction and sequencing DNA was isolated from 5–15 mg dried tissue using a DNeasy96 Plant kit (Qiagen). .. The manufacturer's protocol was modified as follows: tissue was disrupted and then incubated for 12–18 h with gentle mixing at 42°C in 600 µL of the supplied AP1 buffer with 600 µg of protease K added (630 µL total volume).

    Article Title: Multiple Multilocus DNA Barcodes from the Plastid Genome Discriminate Plant Species Equally Well
    Article Snippet: .. DNA isolation and amplification For each sample, we isolated total genomic DNA from approximately 10 mg of dried leaf material using DNeasy 96 Plant Kits (QIAGEN) following manufacturer instructions. .. We performed DNA amplification with various annealing temperatures depending on the primers used ( ).

    Sequencing:

    Article Title: Commercial Teas Highlight Plant DNA Barcode Identification Successes and Obstacles
    Article Snippet: .. DNA extraction and sequencing DNA was isolated from 5–15 mg dried tissue using a DNeasy96 Plant kit (Qiagen). .. The manufacturer's protocol was modified as follows: tissue was disrupted and then incubated for 12–18 h with gentle mixing at 42°C in 600 µL of the supplied AP1 buffer with 600 µg of protease K added (630 µL total volume).

    Article Title: Host ecotype generates evolutionary and epidemiological divergence across a pathogen metapopulation
    Article Snippet: Genomic DNA was extracted from 100 mg of spores per isolate using a DNeasy 96 plant kit (QIAGEN). .. Methods for PCR and sequencing were as previously described in Barrett et al .

    Article Title: Genotyping-by-sequencing markers facilitate the identification of quantitative trait loci controlling resistance to Penicillium expansum in Malus sieversii
    Article Snippet: GBS markers DNA from 50 mg leaf tissue was extracted using DNeasy 96 Plant Kits (Qiagen, Valencia CA, USA) from ‘Royal Gala’ (PI651008), PI613981 and population GMAL4593 (N = 187). .. Library construction using Ape KI [ ] and Illumina HiSeq 2000 (Illumina Inc., San Diego CA, USA) sequencing were completed at the Institute of Biotechnology, Cornell University, Ithaca, NY, USA.

    Article Title: Allantoin accumulation through overexpression of ureide permease1 improves rice growth under limited nitrogen conditions
    Article Snippet: For genotyping, genomic DNA extraction was performed using DNeasy 96 Plant Kit (QIAGEN, Valencia, CA), according to manufacturer's instructions. .. To generate GOS2:OsUPS1 overexpression plants (OsUPS1 GOS2 ), the coding sequence of OsUPS1 (Os12 g0503000) was amplified from rice cDNA (Oryza sativa L. ssp. japonica cv.

    Sampling:

    Article Title: Local adaptation (mostly) remains local: reassessing environmental associations of climate-related candidate SNPs in Arabidopsis halleri
    Article Snippet: Paragraph title: Sampling and DNA extraction ... Sampled leaf tissue was dried in silica gel, and DNA was extracted with the Qiagen DNeasy Plant Mini Kit or DNeasy 96 Plant Kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol.

    SYBR Green Assay:

    Article Title: The Effect of Plant Genotype, Growth Stage, and Mycosphaerella graminicola Strains on the Efficiency and Durability of Wheat-Induced Resistance by Paenibacillus sp. Strain B2
    Article Snippet: DNA was extracted from the plant roots using DNeasy 96 Plant kit (Qiagen, United States), according to the manufacturer’s protocol. .. SYBR Green qPCR assays were carried out in a reaction mixture of 25 μL that contained the following: 12.5 μl Universal Quantifast SYBR Green PCR master mix (Qiagen, United States), 0.3 μM of each primer, 50 ng of DNA, and water up to a volume of 25 μl.

    Marker:

    Article Title: Extensive contemporary pollen-mediated gene flow in two herb species, Ranunculus bulbosus and Trifolium montanum, along an altitudinal gradient in a meadow landscape
    Article Snippet: 10 mg dried leaf tissue with DNeasy 96 Plant Kits (Qiagen, Hombrechtikon Switzerland). .. In T. montanum , repeatability was assessed on 18–41 individuals per marker.

    Incubation:

    Article Title: Commercial Teas Highlight Plant DNA Barcode Identification Successes and Obstacles
    Article Snippet: DNA extraction and sequencing DNA was isolated from 5–15 mg dried tissue using a DNeasy96 Plant kit (Qiagen). .. The manufacturer's protocol was modified as follows: tissue was disrupted and then incubated for 12–18 h with gentle mixing at 42°C in 600 µL of the supplied AP1 buffer with 600 µg of protease K added (630 µL total volume).

    other:

    Article Title: Imaging spectroscopy links aspen genotype with below-ground processes at landscape scales
    Article Snippet: Genomic DNA was extracted from dried leaf tissue using a Qiagen DNeasy 96 Plant kit.

    Article Title: Natural variation in cross-talk between glucosinolates and onset of flowering in Arabidopsis
    Article Snippet: Genotyping by MassARRAY For genotyping, genomic DNA was extracted using the Qiagen DNeasy 96 Plant Kit (Qiagen) according to manufacturer's protocol.

    Transplantation Assay:

    Article Title: Allantoin accumulation through overexpression of ureide permease1 improves rice growth under limited nitrogen conditions
    Article Snippet: Fertilizer was applied at 70N/40P/70K kg/ha after the last paddling and 45 days after transplantation. .. For genotyping, genomic DNA extraction was performed using DNeasy 96 Plant Kit (QIAGEN, Valencia, CA), according to manufacturer's instructions.

    Modification:

    Article Title: Commercial Teas Highlight Plant DNA Barcode Identification Successes and Obstacles
    Article Snippet: DNA extraction and sequencing DNA was isolated from 5–15 mg dried tissue using a DNeasy96 Plant kit (Qiagen). .. The manufacturer's protocol was modified as follows: tissue was disrupted and then incubated for 12–18 h with gentle mixing at 42°C in 600 µL of the supplied AP1 buffer with 600 µg of protease K added (630 µL total volume).

    Real-time Polymerase Chain Reaction:

    Article Title: The Effect of Plant Genotype, Growth Stage, and Mycosphaerella graminicola Strains on the Efficiency and Durability of Wheat-Induced Resistance by Paenibacillus sp. Strain B2
    Article Snippet: External and internal colonization by PB2 was also determined at the 3-leaf (3-L) and flag-leaf (FL) growth stages (GS) on Alixan and Cellule, by the quantitative real-time PCR (qPCR). .. DNA was extracted from the plant roots using DNeasy 96 Plant kit (Qiagen, United States), according to the manufacturer’s protocol.

    Over Expression:

    Article Title: Allantoin accumulation through overexpression of ureide permease1 improves rice growth under limited nitrogen conditions
    Article Snippet: For genotyping, genomic DNA extraction was performed using DNeasy 96 Plant Kit (QIAGEN, Valencia, CA), according to manufacturer's instructions. .. To generate GOS2:OsUPS1 overexpression plants (OsUPS1 GOS2 ), the coding sequence of OsUPS1 (Os12 g0503000) was amplified from rice cDNA (Oryza sativa L. ssp. japonica cv.

    Transformation Assay:

    Article Title: Allantoin accumulation through overexpression of ureide permease1 improves rice growth under limited nitrogen conditions
    Article Snippet: Paragraph title: Plant material, genotyping and rice transformation ... For genotyping, genomic DNA extraction was performed using DNeasy 96 Plant Kit (QIAGEN, Valencia, CA), according to manufacturer's instructions.

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  • 99
    Qiagen dneasy96 plant kit
    Dneasy96 Plant Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dneasy96 plant kit/product/Qiagen
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    dneasy96 plant kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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