dneasy 96 plant kit  (Qiagen)


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    Name:
    DNeasy 96 Plant Kit
    Description:
    For isolation of up to 15 µg per well total cellular DNA from plant tissue Kit contents Qiagen DNeasy 96 Plant Kit 6 x 96 preps 50mg Sample 100 to 200L Elution Volume Plant Cells Tissues Sample DNA Purification Silica Technology Manual Processing 96 well Plate Format 1 to 15g Yield Ideal for PCR Real time PCR Blotting For Isolation of Up to 15g Well Total Cellular DNA from Plant Tissue Includes 6 DNeasy 96 Plates Buffers Reagents RNase A S Blocks 1 2mL Collection Microtubes Caps AirPore Tape Sheets Benefits Pure DNA free from contaminants and enzyme inhibitors Rapid purification of ready to use DNA No organic extraction no ethanol precipitatio
    Catalog Number:
    69181
    Price:
    1739
    Category:
    DNeasy 96 Plant Kit
    Buy from Supplier


    Structured Review

    Qiagen dneasy 96 plant kit
    DNeasy 96 Plant Kit
    For isolation of up to 15 µg per well total cellular DNA from plant tissue Kit contents Qiagen DNeasy 96 Plant Kit 6 x 96 preps 50mg Sample 100 to 200L Elution Volume Plant Cells Tissues Sample DNA Purification Silica Technology Manual Processing 96 well Plate Format 1 to 15g Yield Ideal for PCR Real time PCR Blotting For Isolation of Up to 15g Well Total Cellular DNA from Plant Tissue Includes 6 DNeasy 96 Plates Buffers Reagents RNase A S Blocks 1 2mL Collection Microtubes Caps AirPore Tape Sheets Benefits Pure DNA free from contaminants and enzyme inhibitors Rapid purification of ready to use DNA No organic extraction no ethanol precipitatio
    https://www.bioz.com/result/dneasy 96 plant kit/product/Qiagen
    Average 90 stars, based on 78 article reviews
    Price from $9.99 to $1999.99
    dneasy 96 plant kit - by Bioz Stars, 2020-01
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Local adaptation (mostly) remains local: reassessing environmental associations of climate-related candidate SNPs in Arabidopsis halleri
    Article Snippet: We collected 20 individuals per population and maintained a minimal distance of 2 m (preferably 4 m) among plants to avoid potential clones. .. Sampled leaf tissue was dried in silica gel, and DNA was extracted with the Qiagen DNeasy Plant Mini Kit or DNeasy 96 Plant Kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol.

    Amplification:

    Article Title: Spatial and temporal determinants of genetic structure in Gentianella bohemica
    Article Snippet: .. Amplified Fragment Length Polymorphisms Total genomic DNA from silica gel-dried leaf material was extracted using DNeasy 96 Plant Kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol. .. AFLP fingerprint profiles were generated for (exceptionally 8) 25–33 individuals per population, totaling 253 individuals, following the protocol described in .

    Article Title: Allantoin accumulation through overexpression of ureide permease1 improves rice growth under limited nitrogen conditions
    Article Snippet: Plant material, genotyping and rice transformation T‐DNA insertion line (PFG_2D‐4064.L) was provided by Prof. Gynheung An of Kyunghee University and amplified on a rice paddy field. .. For genotyping, genomic DNA extraction was performed using DNeasy 96 Plant Kit (QIAGEN, Valencia, CA), according to manufacturer's instructions.

    Article Title: Ethylmethanesulfonate Saturation Mutagenesis in Arabidopsis to Determine Frequency of Herbicide Resistance
    Article Snippet: DNA was extracted from leaf samples using a DNeasy 96 Plant Kit (Qiagen USA, Valencia, CA) according to the manufacturer's instructions. .. The DNA sequence of the coding region of the CSR1 gene and approximately 2,000 bp upstream and 500 bp downstream was amplified by PCR as 24 fragments of approximately 500 bp each, with an average overlap of approximately 150 bp between adjacent fragments.

    Picogreen Assay:

    Article Title: High Density Single Nucleotide Polymorphism (SNP) Mapping and Quantitative Trait Loci (QTL) Analysis in a Biparental Spring Triticale Population Localized Major and Minor Effect Fusarium Head Blight Resistance and Associated Traits QTL
    Article Snippet: Leaf tissue samples were collected 10 days post-seeding followed by DNA isolation using DNeasy 96 Plant Kit (Qiagen Inc., Valencia, CA, USA) following the manufacturer’s instructions. .. DNA was quantified using Quant-iT™ PicoGreen® dsDNA Assay Kit (Thermo Fisher Scientific Inc., Bartlesville, OK, USA) and all DNA samples were diluted to 50 ng/µl.

    Real-time Polymerase Chain Reaction:

    Article Title: The Effect of Plant Genotype, Growth Stage, and Mycosphaerella graminicola Strains on the Efficiency and Durability of Wheat-Induced Resistance by Paenibacillus sp. Strain B2
    Article Snippet: External and internal colonization by PB2 was also determined at the 3-leaf (3-L) and flag-leaf (FL) growth stages (GS) on Alixan and Cellule, by the quantitative real-time PCR (qPCR). .. DNA was extracted from the plant roots using DNeasy 96 Plant kit (Qiagen, United States), according to the manufacturer’s protocol.

    Transplantation Assay:

    Article Title: Allantoin accumulation through overexpression of ureide permease1 improves rice growth under limited nitrogen conditions
    Article Snippet: Fertilizer was applied at 70N/40P/70K kg/ha after the last paddling and 45 days after transplantation. .. For genotyping, genomic DNA extraction was performed using DNeasy 96 Plant Kit (QIAGEN, Valencia, CA), according to manufacturer's instructions.

    Incubation:

    Article Title: Composition of fungal soil communities varies with plant abundance and geographic origin
    Article Snippet: To verify proper surface sterilization three 0.5-cm root pieces of each individual plant were excised and surface sterilized as described above and incubated at room temperature on potato dextrose agar plates. .. DNA was extracted using the DNeasy 96 Plant Kit (Qiagen, Toronto, ON, Canada) according to the manufacturer's protocol.

    Modification:

    Article Title: Narrowing down the single homoeologous FaPFRU locus controlling flowering in cultivated octoploid strawberry using a selective mapping strategy
    Article Snippet: .. DNA extraction Genomic DNA was extracted using the DNeasy™ Plant Mini Kit or DNeasy™ 96 Plant Kit (Qiagen Inc., Hilden, Germany) with 90 mg or 45 mg, respectively, of young, developed, leaf tissue according to the manufacturer's protocol and modified as previously described (Lerceteau‐Köhler et al ., ). gDNA was quantified visually using the DNA‐binding dye GelGreen™ (Biotium, Hayward, CA) and standards of known quantity. ..

    Transformation Assay:

    Article Title: Allantoin accumulation through overexpression of ureide permease1 improves rice growth under limited nitrogen conditions
    Article Snippet: Paragraph title: Plant material, genotyping and rice transformation ... For genotyping, genomic DNA extraction was performed using DNeasy 96 Plant Kit (QIAGEN, Valencia, CA), according to manufacturer's instructions.

    Over Expression:

    Article Title: Allantoin accumulation through overexpression of ureide permease1 improves rice growth under limited nitrogen conditions
    Article Snippet: For genotyping, genomic DNA extraction was performed using DNeasy 96 Plant Kit (QIAGEN, Valencia, CA), according to manufacturer's instructions. .. To generate GOS2:OsUPS1 overexpression plants (OsUPS1 GOS2 ), the coding sequence of OsUPS1 (Os12 g0503000) was amplified from rice cDNA (Oryza sativa L. ssp. japonica cv.

    Generated:

    Article Title: Spatial and temporal determinants of genetic structure in Gentianella bohemica
    Article Snippet: Amplified Fragment Length Polymorphisms Total genomic DNA from silica gel-dried leaf material was extracted using DNeasy 96 Plant Kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol. .. AFLP fingerprint profiles were generated for (exceptionally 8) 25–33 individuals per population, totaling 253 individuals, following the protocol described in .

    DNA Sequencing:

    Article Title: Ethylmethanesulfonate Saturation Mutagenesis in Arabidopsis to Determine Frequency of Herbicide Resistance
    Article Snippet: Paragraph title: DNA Sequencing ... DNA was extracted from leaf samples using a DNeasy 96 Plant Kit (Qiagen USA, Valencia, CA) according to the manufacturer's instructions.

    Polymerase Chain Reaction:

    Article Title: Environmental Heterogeneity Explains the Genetic Structure of Continental and Mediterranean Populations of Fraxinus angustifolia Vahl
    Article Snippet: Microsatellite analysis Total DNA was extracted using DNeasy 96 Plant Kit (Qiagen) from up to ten mg of dried leaf tissue following the manufacturer's protocols. .. The PCR conditions followed those used by Morand .

    Article Title: Evolutionary limits ameliorate the negative impact of an invasive plant
    Article Snippet: .. In brief, we extracted DNA using the DNeasy 96 Plant Kit (Qiagen) and performed two multiplexed PCR (five and three loci each) for each individual using identical conditions to ( ). .. PCR products were separated on an ABI Prism 3730xl Analyzer with a ROX 500 internal size standard.

    Article Title: Anthropogenic disturbance as a driver of microspatial and microhabitat segregation of cytotypes of Centaurea stoebe and cytotype interactions in secondary contact zones
    Article Snippet: DNA was extracted from 10–15 mg of silica-dried leaf tissue with a DNeasy 96 Plant Kit and DNeasy Plant Mini Kit (Qiagen) following the manufacturer's protocol. .. Amplifications were performed in a 25-μL volume containing 5 µL genomic DNA (4 ng μL−1 ), 2·5 µ m buffer (10× PCR buffer), 1 m m MgCl2 , 0·2 m m of each dNTP, 0·4 µ m of each primer, 0·25 µ m bovine serum albumin (BSA) and 1 U Taq polymerase (Qiagen).

    Article Title: Chromosome-End Knockoff Strategy to Reshape Alkaloid Profiles of a Fungal Endophyte
    Article Snippet: Molecular methods Fungal DNA was isolated from fresh mycelium using ZR Fungal/Bacterial DNA MiniPrep kit (Zymo Research, Irvine, CA), or using Geno/Grinder 2000 (SPEX CertiPrep, Metuchen, NJ) and DNeasy 96 Plant Kit (Qiagen, Valencia, CA). .. PCR screens were performed using AmpliTaq Gold, and AmpliTaq Gold PCR buffer provided by the manufacturer (Applied Biosystems, Foster City, CA).

    Article Title: The Effect of Plant Genotype, Growth Stage, and Mycosphaerella graminicola Strains on the Efficiency and Durability of Wheat-Induced Resistance by Paenibacillus sp. Strain B2
    Article Snippet: DNA was extracted from the plant roots using DNeasy 96 Plant kit (Qiagen, United States), according to the manufacturer’s protocol. .. SYBR Green qPCR assays were carried out in a reaction mixture of 25 μL that contained the following: 12.5 μl Universal Quantifast SYBR Green PCR master mix (Qiagen, United States), 0.3 μM of each primer, 50 ng of DNA, and water up to a volume of 25 μl.

    Article Title: Allantoin accumulation through overexpression of ureide permease1 improves rice growth under limited nitrogen conditions
    Article Snippet: For genotyping, genomic DNA extraction was performed using DNeasy 96 Plant Kit (QIAGEN, Valencia, CA), according to manufacturer's instructions. .. T‐DNA insertion was then confirmed through PCR using the primers listed in Table .

    Article Title: Ethylmethanesulfonate Saturation Mutagenesis in Arabidopsis to Determine Frequency of Herbicide Resistance
    Article Snippet: DNA was extracted from leaf samples using a DNeasy 96 Plant Kit (Qiagen USA, Valencia, CA) according to the manufacturer's instructions. .. The DNA sequence of the coding region of the CSR1 gene and approximately 2,000 bp upstream and 500 bp downstream was amplified by PCR as 24 fragments of approximately 500 bp each, with an average overlap of approximately 150 bp between adjacent fragments.

    DNA Extraction:

    Article Title: Local adaptation (mostly) remains local: reassessing environmental associations of climate-related candidate SNPs in Arabidopsis halleri
    Article Snippet: Paragraph title: Sampling and DNA extraction ... Sampled leaf tissue was dried in silica gel, and DNA was extracted with the Qiagen DNeasy Plant Mini Kit or DNeasy 96 Plant Kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol.

    Article Title: A TILLING Platform for Functional Genomics in Brachypodium distachyon
    Article Snippet: Paragraph title: Genomic DNA Extraction and Pooling ... Genomic DNAs were isolated using the DNeasy 96 Plant Kit (Qiagen, Hilden, Germany).

    Article Title: Narrowing down the single homoeologous FaPFRU locus controlling flowering in cultivated octoploid strawberry using a selective mapping strategy
    Article Snippet: .. DNA extraction Genomic DNA was extracted using the DNeasy™ Plant Mini Kit or DNeasy™ 96 Plant Kit (Qiagen Inc., Hilden, Germany) with 90 mg or 45 mg, respectively, of young, developed, leaf tissue according to the manufacturer's protocol and modified as previously described (Lerceteau‐Köhler et al ., ). gDNA was quantified visually using the DNA‐binding dye GelGreen™ (Biotium, Hayward, CA) and standards of known quantity. ..

    Article Title: Anthropogenic disturbance as a driver of microspatial and microhabitat segregation of cytotypes of Centaurea stoebe and cytotype interactions in secondary contact zones
    Article Snippet: Paragraph title: DNA extraction and molecular analyses ... DNA was extracted from 10–15 mg of silica-dried leaf tissue with a DNeasy 96 Plant Kit and DNeasy Plant Mini Kit (Qiagen) following the manufacturer's protocol.

    Article Title: High Density Single Nucleotide Polymorphism (SNP) Mapping and Quantitative Trait Loci (QTL) Analysis in a Biparental Spring Triticale Population Localized Major and Minor Effect Fusarium Head Blight Resistance and Associated Traits QTL
    Article Snippet: .. Leaf tissue samples were collected 10 days post-seeding followed by DNA isolation using DNeasy 96 Plant Kit (Qiagen Inc., Valencia, CA, USA) following the manufacturer’s instructions. .. DNA was quantified using Quant-iT™ PicoGreen® dsDNA Assay Kit (Thermo Fisher Scientific Inc., Bartlesville, OK, USA) and all DNA samples were diluted to 50 ng/µl.

    Article Title: Composition of fungal soil communities varies with plant abundance and geographic origin
    Article Snippet: For very thick roots, the amount of root material was adjusted accordingly to avoid overloading the DNA extraction column. .. DNA was extracted using the DNeasy 96 Plant Kit (Qiagen, Toronto, ON, Canada) according to the manufacturer's protocol.

    Article Title: Allantoin accumulation through overexpression of ureide permease1 improves rice growth under limited nitrogen conditions
    Article Snippet: .. For genotyping, genomic DNA extraction was performed using DNeasy 96 Plant Kit (QIAGEN, Valencia, CA), according to manufacturer's instructions. .. T‐DNA insertion was then confirmed through PCR using the primers listed in Table .

    Isolation:

    Article Title: A TILLING Platform for Functional Genomics in Brachypodium distachyon
    Article Snippet: .. Genomic DNAs were isolated using the DNeasy 96 Plant Kit (Qiagen, Hilden, Germany). .. All genomic DNAs were both quantified on a 1% agarose gel with λ DNA (Invitrogen, Carlsbad, CA, USA) as a concentration reference using a NanoDrop spectrophotometer 2000 c (Thermo Fisher Scientific, MA, USA).

    Article Title: Chromosome-End Knockoff Strategy to Reshape Alkaloid Profiles of a Fungal Endophyte
    Article Snippet: .. Molecular methods Fungal DNA was isolated from fresh mycelium using ZR Fungal/Bacterial DNA MiniPrep kit (Zymo Research, Irvine, CA), or using Geno/Grinder 2000 (SPEX CertiPrep, Metuchen, NJ) and DNeasy 96 Plant Kit (Qiagen, Valencia, CA). .. Plasmid DNA was isolated from bacterial cultures using the ZR Plasmid Miniprep-Classic kit (Zymo Research, Irvine, CA).

    Purification:

    Article Title: Composition of fungal soil communities varies with plant abundance and geographic origin
    Article Snippet: DNA was extracted using the DNeasy 96 Plant Kit (Qiagen, Toronto, ON, Canada) according to the manufacturer's protocol. .. The extracted DNA was cleaned and purified using the Genomic DNA Clean & Concentrator™ Kit (Zymo Research, USA).

    Sequencing:

    Article Title: Allantoin accumulation through overexpression of ureide permease1 improves rice growth under limited nitrogen conditions
    Article Snippet: For genotyping, genomic DNA extraction was performed using DNeasy 96 Plant Kit (QIAGEN, Valencia, CA), according to manufacturer's instructions. .. To generate GOS2:OsUPS1 overexpression plants (OsUPS1 GOS2 ), the coding sequence of OsUPS1 (Os12 g0503000) was amplified from rice cDNA (Oryza sativa L. ssp. japonica cv.

    Article Title: Ethylmethanesulfonate Saturation Mutagenesis in Arabidopsis to Determine Frequency of Herbicide Resistance
    Article Snippet: DNA was extracted from leaf samples using a DNeasy 96 Plant Kit (Qiagen USA, Valencia, CA) according to the manufacturer's instructions. .. The DNA sequence of the coding region of the CSR1 gene and approximately 2,000 bp upstream and 500 bp downstream was amplified by PCR as 24 fragments of approximately 500 bp each, with an average overlap of approximately 150 bp between adjacent fragments.

    Plasmid Preparation:

    Article Title: Chromosome-End Knockoff Strategy to Reshape Alkaloid Profiles of a Fungal Endophyte
    Article Snippet: Molecular methods Fungal DNA was isolated from fresh mycelium using ZR Fungal/Bacterial DNA MiniPrep kit (Zymo Research, Irvine, CA), or using Geno/Grinder 2000 (SPEX CertiPrep, Metuchen, NJ) and DNeasy 96 Plant Kit (Qiagen, Valencia, CA). .. Plasmid DNA was isolated from bacterial cultures using the ZR Plasmid Miniprep-Classic kit (Zymo Research, Irvine, CA).

    Software:

    Article Title: Evolutionary limits ameliorate the negative impact of an invasive plant
    Article Snippet: In brief, we extracted DNA using the DNeasy 96 Plant Kit (Qiagen) and performed two multiplexed PCR (five and three loci each) for each individual using identical conditions to ( ). .. Individuals were genotyped with GeneMapper TM software (Applied Biosystems).

    Article Title: High Density Single Nucleotide Polymorphism (SNP) Mapping and Quantitative Trait Loci (QTL) Analysis in a Biparental Spring Triticale Population Localized Major and Minor Effect Fusarium Head Blight Resistance and Associated Traits QTL
    Article Snippet: Leaf tissue samples were collected 10 days post-seeding followed by DNA isolation using DNeasy 96 Plant Kit (Qiagen Inc., Valencia, CA, USA) following the manufacturer’s instructions. .. SNP genotyping data was analyzed using Genotyping module of GenomeStudio software package (Illumina Inc., San Diego, CA, USA).

    SYBR Green Assay:

    Article Title: The Effect of Plant Genotype, Growth Stage, and Mycosphaerella graminicola Strains on the Efficiency and Durability of Wheat-Induced Resistance by Paenibacillus sp. Strain B2
    Article Snippet: DNA was extracted from the plant roots using DNeasy 96 Plant kit (Qiagen, United States), according to the manufacturer’s protocol. .. SYBR Green qPCR assays were carried out in a reaction mixture of 25 μL that contained the following: 12.5 μl Universal Quantifast SYBR Green PCR master mix (Qiagen, United States), 0.3 μM of each primer, 50 ng of DNA, and water up to a volume of 25 μl.

    Agarose Gel Electrophoresis:

    Article Title: A TILLING Platform for Functional Genomics in Brachypodium distachyon
    Article Snippet: Genomic DNAs were isolated using the DNeasy 96 Plant Kit (Qiagen, Hilden, Germany). .. All genomic DNAs were both quantified on a 1% agarose gel with λ DNA (Invitrogen, Carlsbad, CA, USA) as a concentration reference using a NanoDrop spectrophotometer 2000 c (Thermo Fisher Scientific, MA, USA).

    Spectrophotometry:

    Article Title: A TILLING Platform for Functional Genomics in Brachypodium distachyon
    Article Snippet: Genomic DNAs were isolated using the DNeasy 96 Plant Kit (Qiagen, Hilden, Germany). .. All genomic DNAs were both quantified on a 1% agarose gel with λ DNA (Invitrogen, Carlsbad, CA, USA) as a concentration reference using a NanoDrop spectrophotometer 2000 c (Thermo Fisher Scientific, MA, USA).

    Article Title: Composition of fungal soil communities varies with plant abundance and geographic origin
    Article Snippet: DNA was extracted using the DNeasy 96 Plant Kit (Qiagen, Toronto, ON, Canada) according to the manufacturer's protocol. .. The amount of DNA was measured using a BioTek™ Epoch™ Microplate Spectrophotometer (Winooski, VT, USA).

    Sampling:

    Article Title: Local adaptation (mostly) remains local: reassessing environmental associations of climate-related candidate SNPs in Arabidopsis halleri
    Article Snippet: Paragraph title: Sampling and DNA extraction ... Sampled leaf tissue was dried in silica gel, and DNA was extracted with the Qiagen DNeasy Plant Mini Kit or DNeasy 96 Plant Kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol.

    Concentration Assay:

    Article Title: A TILLING Platform for Functional Genomics in Brachypodium distachyon
    Article Snippet: Genomic DNAs were isolated using the DNeasy 96 Plant Kit (Qiagen, Hilden, Germany). .. All genomic DNAs were both quantified on a 1% agarose gel with λ DNA (Invitrogen, Carlsbad, CA, USA) as a concentration reference using a NanoDrop spectrophotometer 2000 c (Thermo Fisher Scientific, MA, USA).

    High Throughput Screening Assay:

    Article Title: High Density Single Nucleotide Polymorphism (SNP) Mapping and Quantitative Trait Loci (QTL) Analysis in a Biparental Spring Triticale Population Localized Major and Minor Effect Fusarium Head Blight Resistance and Associated Traits QTL
    Article Snippet: Leaf tissue samples were collected 10 days post-seeding followed by DNA isolation using DNeasy 96 Plant Kit (Qiagen Inc., Valencia, CA, USA) following the manufacturer’s instructions. .. High-throughput SNP genotyping was carried out using wheat 90K Infinium iSelect SNP Assay and rye 10K SNP Assay (provided by KWS LOCHOW GMBH, Bergen, Germany).

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  • 90
    Qiagen dneasy 96 plant kit
    Dneasy 96 Plant Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dneasy 96 plant kit/product/Qiagen
    Average 90 stars, based on 59 article reviews
    Price from $9.99 to $1999.99
    dneasy 96 plant kit - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

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